Document jyqOqzMzd5qZoBM8wZ6arjNnO
ORUNOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE
1-Decanol, 3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluoroDaphnia magna Reproduction Test (21 d) Limit Test `according to OEC(Da-dGoputieddeSlenpet2e1m1befror1T9e9s8t)ing of Chemicals
Sponsor TelomeArsRaenseGauracshsProject OKCIuNorursTiRnIES LTD
Dupont
Author Martina Noack
Testing Facility
DFRU.RUANNOGAECWKA-NLDaTBEOBRIAOTLOOGRIUEM K3at1h1e-5Pa7uiSuasr.sStre.dt1
Laboratory Project ID
SPrtoujdecytN-oN.o.
0D2R0E7B1A9101KT
Study-No.of theStudyPlan: DREB411-
Page 1010
Study Completed on 2003-10-17
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Statement of GLP Compliance
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'OR.UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
1Re-pDoerctanol, 3.344.5.5,6,6.7,7,8,8.9.9,10,10,10-hoptadecafluoro-
Page dof 50
a`cDcapohrndiiangmtaogOnaECReDprGoudiudecltiinoen2T1e1st (21 a, Limit Test _--
PSrtoujdoycNtoN.o. D0R2E0B7A1191C1K CuoreDRE
Personnel Involved
Study Director S(cAineanityitsitc:al part) Technical taf:
Quality Assurance Unit Deputy. TDiersetcitnogrFoafcil:
Marina Noack. (Biologist) T(ChhoemmaicsalGefEfnkgei:neer) AGuneknedGoelhilnerKtraetzig Jens Lange (GuBdirougnisM)ohrmann-Kalabokidis (`BSiuosoagninset)Becker
Or. Udo Noack (Biologist)
------------------------
S 'DR.UNOACK-LABORATORIUM e FUR ANGEWANDTE BIOLOGIE
Report 1-Decanol, 3.34,4,5.56.6.7,7,8,8.9.9,1010,10-heptadecafluoro-
Page 5.50
a`cDcaoprhndiiangmtaogOnaECReDprGoudiudecltiinoen2T1e1st (21 d, Limit Test _--
PrSotjuedcytNoN.o. D0R2E0B7A1191C1K
Sudo Rem
Table of Content
Page
To Page Statement of GLP Compliance
1 2
PSetrastoenmneenlt oIfnvQoulavleidty Assurance Unit
+3
Table of Content Listof Tabios
5
UstotFigwes
1
`Summary
000000000000 6 7
2 3
MeChtahraocdterisation Data ofthe Tost lem
" 2
4 a
Results Protminary Tests.
19 19
a2 421
RReepsruoldsucotfitvheeODuetfpiuntitve Tost
2 2
422 423
NiummmobbeireofJuAvbeorntieeds Eggs Producing no Juvenies
2 2
424 425
iFimrmsotbAiplipseaatrieonncoef tohfeJuPvaerneiniteasl
2 2
426 427
PGrreoswetnhc(eTootfalMaLleensgth and Dry Weigh) of the Parent Animals
2 2
428 429
OWcactuerrrPeanrcaemoefteErpshippia (Winter Eggs)
2 2
4210 4211
CAocnuctleuIsmimoonbsilsation Test of the Reference ltem
2 2
5 6
VLitaerlatuCrre|iteRreifaerences
2 2
7 8
WObasteerrvaPtairaomnestaetrsT.est and Control Groups.
2 2
o
hGepCta--deAncaalfyisviosroo-f 1-Decancl, 3.3.4.4.5,5.6.6.7.7.88.99, 10.10,10-
EY
91 Metho 92 Method Validation
ES 3
921 Lineariy
922 03
RoQsuaunlttifsication Limit and Detection Limit
3 33
04 95
ChCraloimraattioognrCaumrsves
3 " a
10 Cheerpttiafdieccataefioufo1r-oD-eAcnaanolly,sis3.3.4.4.5.56.6.77.8.8.99,1010.10-
a
1" `GLPCertiicats of DR UNOACK-LABORATORUM
50
eee------e-- e -- --
_--
ORUNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
Re1p-oDerctanol, 3,344,5,5.6.6.7,7,8,8.99,10.10,10-heptadecafluoro-
Page6.0 50
--a`Dc_ac--pohrndiiangm1aogOnaECReDprGouidduecltiinoen2T1e1st (21, Limt Test
SPrtoujdeycNtoN.o. Suse
D0R2E0B7A1191C1K Rem
Table1:
Table 2:
Tai 3:
Table 4:
TTaabbllee 56::
TTaabblloe 87:.
Tables:
Table Tavie
10: 11:
Table 12:
Table 13:
TTaabbllee 1154::
Table Table
16: 17:
Table 18:
Table 19:
Table 20:
Figure 1: Figure 2: FFiigguurree 43.. FFiigguurree 65:: FFiigguurree 78: FFiigguurree 19:0:
List of Tables
Page
SImumombmiiasraotyfioTnesRtatleesmofRtehleaPtreedlEifmfiencatrsyiAnctuhteeLiImmimtbCioinscaetntiroantTioensst (%)
19
Number Number
of of
Juvenies Immobie
in the Control, Juveniles
Solvent
Conirol
and
Test
Groups
after
21
Days
21 2
FNiursmtbAeprpeofarAabnorctoeodfEJgugvesniles in the Individual Groups.
2 2
OTobtsaelrvLaotnigoinhsaantdthDeryCoWnieroil Ggorfohtuhtpe Parent Animals
2 2
OObbsseerrvvaattiioonnss aatt tthhee LSiomlivteCnotnCcoennttrroaltiGroonup.
27 2
pH-Valuos Dissolved Oxygen Concentration [mgiL]
2 2
TTeomtpaeHraartdunrees[sC[)mg/L] as CaCO3
2 2
CDointcieonntroaftiSoanmsplofesthoefAScttoicvke
Solution Ingredient
1-Decandl,
33.44.5.5,66.7,7.8899,10,
31 34
CCoonncceennttrraattiioonnss ooff tthhee AAccttiivvee IInnggrreeddiieenntt 11--DDeeccaannoall,, 3333..44.4..45..55..65,6..676.77.878.986.9901.100,.
36 38
G33e4o.m4e5t5r6i,c6M7,e7a.n8.E8x9p.o9s,u1r0e,1C0o,n1ce0n-threapttiaodneocfat1i-uDoercoan-ci,
"0
3T3i4m,e4.W5e5i6g,h6t.7e.d7.M8e,a8n.9E.x9,p1o0s.u1r0e,1C0o-nhceepnttardaetciaofniovfor1o--Decandl,
a
List of Figures
Page
CCoouurrssee oofftThoteaTleNstumlbleemrCoofnHcaetntcrhaetdioJnuvdeurniinegs3afRtern2e1waDlayIsn.tervals:
8 0
CCaallbbrraattioonn CCuurrvvee ooff tthhe TTeesstt tleamm
" 2
CChhrroommaattooggrraamm ooff SStaamnpdlaerdatofOhT,esdtalye1m4
a3 "
CChhvormaotmogaratmoooffgSCaornmtaprlomle,
al 8 h, day 16
day
16
Pe 6
CChhrroommaattooggrraamm ooff SSotlovceknStolCounttiroonl,,ddaayy1166
a Ps
- DR.UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
Report 1-Decanol, 3,3,44,55,6,67,7,8,8.9,9.10,10,10-heptadecafiuoro-
Pago 7 of 50
`a--Dc_acpo--hrnciiangmtaogOnEaCReDe prGouidduece ltiinoen2-- T1e1st (-- 21 d,-- Limit Test
PSrSotjuuedcytNeoN.o.DD0RR2E0EB741191C1K
1 Summary
hTohpetaDdaepchanfiiauormoa-gn(aBatRcehprNood.ucPtOiOoInODTTe)stwa(2s1 c4o)ndoufci1e-dDoaccacnoorld,in3g.3t,o6t4h,e5,O5.E6C.6D.7,G7u,i8d8e,i9n9e.10N,o.10,21101B(SIoOpLtOeGmEoienrSa1r9s9t8e)dt,frGoomrm2a0n0y3.-06-30 (0 2003-07-21 al DR.U.NOACK-LABORATORIUM FUR ANGEWANOTE
sfTtoearstttichseeylxsiptmioetsmcuorwneacsemnetDrtaahptohidnoni.waictmohantgrrenolnaeawSnaTdlRsoAoflUvtSehnet(Ctcleosontntremole5)d.AiLa10teestvteesrsttyaorr4tg8tahnheiyswwmaess,recian2rdrifvoiiedd2u4aoluhlt.youhrTeshlidosldw.eexrApoosusesumeride oumfetdtih7fo5icdualthtaisedurbebsdetaaepnncpercsoh.aocsOhepnaesbnarasenecddomcolmnoestnehddeesfdyinsidtnietnmhgses,OowfEitpChrDealnigdmuiiwndiaatrnhyociuentvdehosetcaiugdmastepinaontcse.o.nThahaqeduyabtheiaodntotbxeosiottneydafuronsidioondrg pG`rseeevlmiiiamsiitnnagatritycesaitnnvldeesmftilgocawot-nitcohenrnsot.urgafhtaiicoenodslwtooenrgsei.vceaDurisfeefpedrroebdnuytctifhbleloewh-ittgehhsrtovouilglahetmitteysctoonfdceehsnoitgrnatste,sitonwtseh.mi.cDhuTrhhieanrdgefbtoehraeesnaefsufenemsitis.n rsteactiocmmmeentdhaotdiownassoffotuhnedOtoECbeD mgourideansucietadbloec.umTehnettoenstadqeusaiigcntohxiacditybteeen schotosfeidnifnfiincgualtccsourbdstaanncceesw.ith SfTeohsutebeisitmtuydyltehwveeal:sstnucdoaymriiwneaadslocu0at.r1rw3iietmdhgoountteeswtliilmfhietamcos.onlcveWennitttr.hatriMooegntahroafdntotholetwthaeesstouewsme,sdolfcuohrboittshyeenapnriednpeahlriaagtthiioovnnolofaoftittthhyee owsfattotcehker ssoollvieinotncoanntdrolthreeptiecsattetsewmasconce0.1ntmrLaItiLon. The methanol concentration in the test roplicates and tho
ocTfahpteahceaiitpmyaroaefnntdiahloe,thDieamrpehtneosiftapirReoemdp-urrcoetdliuaoctnteoidoenffirfTsetecstbtsroooovdn,epraa2br1oarmdteaetydesrwesgagsssutcaohndaassismbemosodsbyitlwheeeijfgufhve,ecnitllseensog,nth,thiemrmeopbrioidsuacttiioonn 0fT.uh6oe.roc-1o2nw. cae2sn4tdraeanttdeior4nm8ionfhe. dtThdheueraicmntegiavtsehuierniegnr4gep8deirhenitloads1st-iDwnegepcreaenrdilbo,edts3ww3ei.e4tn,h4t5h.et5h.es6t,su6td.uyd7y.d.7.aT8.h06ey.9-ss.2a9m..p18l0i-.n1g01,0i1n0at-enhrgveap1ltsa4-dweec1r6a.eThe concentrations in the corresponding stock solutions were aiso analysed.
fA3o3sl.l4oe.wx4ip5neg.c5ia.o6d,s6e7tch.oe7n.d8an.oa6lr9yd.te9ir.ca1pl0o.lf1yi0nn,do1im0ni-gahslewpretelagldreedcseasmfioionunsoircovu-arvtteehdewitithhantcaodleufofoicutionendtthsceor*nhci>egnh0t.rv9ao8tl5ioa(ntssyeGeorffoig1pu-erDedac1adnoaow)n,dn table 19). Detoafithse analytical results are given in part 9.
Using the calculated
analytically to be 45.3
pvgeri1fi-eDdecacnoonlc,ent3r.a3t.i4o4n.s5,t5h6e6.7g.e7o,m8e.t8r,i9c.9m.1e0a.n101e0x-phoespulraedeccaofnlcueonrtora-tLioanndwaasl.
e(escuts)l.evelAss
are an
based on atemative,
fitghuereti1me(fwoeidgehtaelds
please refer to mean measured
page 32 (equation concentration was
1) and page calculated (0
40 ba
54.519 211 (fr deta please refer o page32 (equation 2.3) and page40 (sult),
e eee OR. NOACKLAe BORATORIA FUR ANGEWANOTn E B0LOGIE
ODReapopohrcntiaa nmaognla,R3pr3ad.u4ci4on.T5es5t,(2616)7L7i1kb,hTp8et8ead9sc1at0i.uo1re0-
Pagesorso Projecto. 0207130K
scoring 1 OECD Gideine211
Supt. OREBHTI1
5 2220
1" Measuring Period
iSzz2?e
88141a v=00009. oozes 2s
vl mee
6 mow x wn w we wm
Time [h]
5 222
2" Measuring Period
io 2
HE
85S8
.
3 8 2| oer y=00003 -0.02+280855x3
oo
os wow om ow x
Time (h]
2 ow
2220
3" Measuring Period
fo
$ 8% 22,
8 1p Tl =0w00e0o2s7m-0s022.61x63+%
os wow om ow
Time 1)
) % ow ow
Figure 1: Course fh Test Har Concentration during3 Renewal nerves
ee
-- Fs ANTSh
Prorat on aazocac
SLi rtshri wieis
T he confit of vara rn uns he mn umber of ing fin rocked sr sre
+ Gg E
TRA AA 9
eR TR JRE
TATED
ml m i--L e --
apean com otcrc
A Drone --
+ RTon a tKO1RSA STI U5 IRT. 0 eo gn
NET--------------".
Hts SATE IST
eoSo] ra rIe a--Sr -- Sotvent Conta centration F[inmeaedoreness tomo|vmromo|io oyun||omnme|]
[oirmrmmeecatiesswoneso| r| ortoom||omnroo o|| snwoaa]|
ip npn
ee
ni trom
OR UNOACK-LABORATORIUM FOR ANGEWANOTE BIOLOGIE
Re1p-oDerctanol, 33.44,55,6,67.7,8,89.9,10,10,10-heptadecauoro`aDcacpohrndiiangmtaogOnEaCReDprGouidduecltiinoen2T1e1st (21 d), Limit Test
Page 100150 PStruodjye-Ncot.o.D0R2E0B741191C1K
(SAtaNtOsViAc)a:l Bsoingfneirfricoannicetesoft (tnheumabcehrieofvejduvednaiteas)were controlled by One Way Analysis of Variance Iinmmoorbdieirsattoiopnrotveesttahcecovrdinag otofiOtEheCdlDos2t0is2y(s1y t9e99m, aDnrdaf}tewstascocnadririieodnsouattwitthhe ptoetsatisnsgiufmacditiyc,hraonmaatceuatse ftheervenaceytreamngoenocfe1p.e0r10m2o.nt5h.mgTlhLeacEcoCrdionfgtfhoeDoIfNer3e8n4c1e2fLe3m0afto1rd.e49tamg.seafetepra2r4th4o.2u.1r0s).was wiki Wdeatteerrmiqnueadliftoy bpearwiatmheitnetrhse aacscoppH-tvaablluef,isd.issolved oxygen. water hardness and temperature were
------------------------------_
LL
OR.UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
Report `1D-aDpehcnainaoml,ag3n3a.4R4e,p5r5o,d6u,c6t7i,o7n.8T,es8t9.(.2110d,)1, 0L,i1m0i-thTeepsttadecafluoroaccording to OECD Guideline 211
Page 110150 PStruodjye-cNto.o. D0R2E0B7A1491C11K
2 Characterisation Data of the Test Item
TEST ITEM
1-Decancl, 3,3.4.4.5,56.6,7.7.88.9.9.10.10, 10heptadecaforo-
Batch Number
Pooioot
CASRN
678:30.7
Chemical characterization 3.34.4.55.6,6.7.7.8,89.9,10.10.10-heptadecafluorodecane-1-ol (EINECS)
purty
`Cs8u-pmepreturofrloualokryolaetkhyalnectt:ha9n9i.93.9%% ((aaf/)a.)
Appearance
Yolowish sod wax
Water solubity
113347 ggiLlaatt 2152ddeeggrreeeCeC., SSiidd 5293 ggLL,. nn==187(D(uDPuoPnot,nt2020020)2)
Stabity in water
Stable at test conditions
Density
Ca. 17 glem
Expiry date Recommended storage Storage attesting facilty Retention Identification parameter attesting faciity
Unlimited if stored under the recommended conditions Room temperature (20 C). protected from moisture. Room temperature, protected fom moisture and light Atleast 1g has boon retains,
Name, batch number and colour.
The fast tem and the information concerning the test tem were provided by the sponsor.
DR UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
Report 1-Decanol, 33.4,455,6,67,7.8,89,3,10,10,10-heptadecafluoroa`DcacpohrnciiangmatogOnaECReDprGoudiudecltiinoen2T1e1st (21d), Limit Test
Page 120150 PrSotjudeyc-tNoN.o. D0R2E0B741191C1K
3 Method GUIDELINE
TYPE AND PURPOSE OF THE TEST
TEST sysTEM Tost organism Reason for the selection of the test organism origin Culure
Culture medium Foeding
OECD Guideline 211 or Testing of Chemicals adopted September 1998
Refefpercotdsuocftion test on Daphnia magna STRAUS to determing the 1-Decanol, 3.3.4.4.6,56.6.77,8.8.99.10,10,10-heptadecafluoronrleevperlodoufcti0o.1n3 magnldLiamsmolbiimlitiscaotnicoenntorfataiponh.ids at the water salubilly 0.13mg/L were chosen in relat0itohne water solubity level and according o the sponsor's request,
`Daphnia magna STRAUS (Clone 5) Daphnia magna STRAUS belongs, in accordance with the study guideline, 10 the suiable Daphnia types and are bred nthe test faci. Institut for Wasser. Boden- und Lufthygiene dos Bundesgesundheltsamtes, Correnspiatz 1, D-14195 Berlin 21012:C3L(tgolmapsesravteusrseeslsof2wi0th-a2p5proxCiamraotetloyler1a.t8eLdc)u,ltinuraen minecduibuamto,rat16 h Humination, umination srengh max. 20 ymol mi" E1l6e0n1d0t M1i8.0 macgcoCradiCnOgMtLo Elendt (1990), modified o a total hardness of 5-7 x weekly ad I6itum with a mix of Desmodesmus subspicalus and Chlorella vugars, with an aigao col density of>10collsimL.
DR UNOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE
Re1p-oDerctanol. 3.344.55,6,67,7.8,89.9.10,10.10-hoptadecafluoro-
Daphnia magna Reproduction according to OECD Guideline
Test 211
(21
d,
Limit
Test
Page 13.0150 PrSotujdeyc-tNNo.o. D0R20E7B1161C1K
TEST ITEM Pro-reatment Stock solution Dispersion treatment Limit concentration
coNTROL
SOLVENT CONTROL
TEST METHOD Test duration Test vessels Volume of the test medium Divion water Number of study organisms Number of rpicates. Aatgteesotfbheegisntudy organisms
1-Decancl 3.3,4,4.5,56.6.7.788.9.9.10.10.10-heptadscaflvoro"hTohmeogteentitsaetmonw.as heated 10 50- 70 C before weighing for TAshteosctkocskolsuotliuotnioonfw1.3asgnottestloengemr usweadstphraenp2a1re.d in methanol, Agtation h0.e1p3tamdegca1i-iDueocraon-cL,di3s3s.o4l.v4e.d5,i5n60..61.7m.L7.8m8o.t9h.a9n.1o0i,l10(,01.10-mL. of the su.tock solution were mixed with dition water to give a final volume of 1
c1o0nrdeiptliiocnasteasswtihtehtSesuttgornouwp.ater without test em tested under same.
t1e0stregprloiucpa.teMsewtihhaonuotl e(0s.t1 tMeULm;teHstPeLdCunGdreardiseantmeGrcaodned,it>io9n9s.a8s%t,heJ.T. Baker) was used. Semi-statc, renewaloftest mediumovery48 h 210 Glass beakers (5 (IDx) 8 (4) cm), 100 mL. covered ith parafi
somL E1l6e0n1i 1Ms8,0magccoCradiCngOt.o ELENOT (1890), moilefoda otalhardnessof
10 animals, individually held 10 per concentration, solvent control and control 2-24
J
DR UNOACK-LABORATORIUM FUR ANGEWANOTE BIOLOGIE
Report D1-aDpehcnainaolm,ag3n.3a4.R4e,p5r5o,d6u,c6t.i7o,n7,T8e,s8t9(.291.d1)0,,1L0i,mi1t0-Theespttadecafluoroaccording to OECD Guideline 211
Page 14 of 50 PSrtoujdeycNtoN.o. D0R2E0S7A1T91C1K
Foecing
Tost temperature mination Photoperiod
-a0i2ywmigthCaiDgaaphDneisamaonddedsamyu.s subspicatus and Chiorolla vigaris, 0.1 a"Tnhde Cfholoodreqlulaanvtuligawraiss (a0D.a2s-4m0o.d93esmmLu)ssussupbsepnisciaotnuspe(r0.f2es4t v0es.s6e8l.mLIi). avalrtieesdt agcrocuoprsdoinntgoantyhepagrrtoicwutlharofdatyh.e Odanpdhnaiydss,bobtuwteweanswathteerseaxmcehafnogre. `tahnedaclogvaecriwnegrwiithntnreodwucpeadrbafyimp.rickinag patie ip through parafim 18-22C. 2 C constant Max. 16-20 yom 5 16/8 hours light dark cycle
REFERENCE TEST
Reference tom Puity Batch No. Storage stablty Test concentrations, Rangeofvalty Tost duration Test vessels Volume of the stuoy medium Dituion water pNourmcboenrceonftrsattuisoyniocrognatnrioslms. Number of animals per tostvossel Number of parallel samplos
(Aacrce.fteoDreIncNe t3e8st41w2as21c1o)nwdituhctaetdeasst dSurtataioAncoufte24ImhmoonbcilespaetriomnoTnetsht in toerstdiengr0faprcoiv.e thevay of the test system and test conditon at the. Potassium dichromapt.ea.(MeRck) 100% K 26074764 2006.02.28 058-10-18-32-m5ol8l 1.0 mg < ECa (24 hours) < 2.5 moll 20,2003.07-08 10 2003-07-09 Glass beakers (5 cm 1D x 8c H), 50 mL. volumewil be usec. 20 mL AccortdoiEnECg 92/69 L383AC.2. Annex 20 animals, dided into 2 paralel samples, each with 10 animals
10
2
SE
DR.UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
Re1p-oDerctanol. 33,44,55,6,67,7.8,89.9.10,10,10-heptadecafivoro-
Page 15.0150
`a_Dc--ac_po--hrndiiangmtaogOnEaCReDprGouidduecltiinoen2T1e1st (21 , Limit Test
PStruodjyeNcot.o. D0R2E0B741191C1K
Sue DRemm
TEST DESCRIPTION
OTFYPMEEAANSDURFERMEEQNUTESNCY AND OBSERVATIONS. Measurements Observations
"tThheetresetprtoedmuctofio0n.s1t3udmywLasatpteherfwoartmeerdswoiltuhbialtnyolmeivneall concentation of o2b-l2a4inehdoubrysroeimdoavpinhgitdhse mwoetrhearusaendimfaolrsthtewitecset.wiTthhieny2h2adh. been f1o0rdtahpehsroildvsenitncdoivnitdruoalllaynhd ceonwtreorleugrsoeupdsf.orFetahdeiinmgiascosnpceecnitfriaetdioanboavne.d Adlalrtkecstycvlees1s6el/s8we)reatp1la8c-e2d2inCa,n a.c2cliCmcaotnesdtainntc,ubation room (ight EvSoelrvyen4t8chonttrhoe saonldutcioonntrsolw,ere renewed atth limit concentration, dOebsscerrviabteidonbse,lowm,easurements and evaluation were carrid out as DAIrNef3e8r4e1n2ce2t1e1stiwnoarsdeprer0foprrmoevdeotnhcevealpiedritmyoonftthheactceostrdsiynsgtteom andi test conditions at the testing fac.
otDniesmesporeelrpvaletiducraotexeywogefertnehecmoecnoacnsetnurtrir,eadttihooenn,cseoHl-avvewanetleuckeo,nst,irnotloftraaelnsdhhaahrneddnteoeslssdt mafeneddmiw,atienr Tcohneceonxtyagteinonconcentration shouldbe > 3 mg at the beginning and Tcuhreintgottalhohafersdtness should be above 140 mglL CaCO "InTihtiealpaHn-dvfainealsphHouvladlbueesisnhtohuekadnbgeo< o1f.5 6 -un9i.ts.Normally deviations of tThheertmeomnpyegrraotgurraephindtuhreinignctuhbeatteosrtwas recorded with a M`orMtaoloroffppaaarreelnnttiDDaaptphhnnyiiaa was observed and recorded at least at w`veerreyuwanterar1eb0nmelwoavle.e Dafatpehrngioanweeraegictoantsioindewrietdahtolabbeordaetaodr,yfpint.heDyead specimen were removed.
------------------
DR UNOACK-LABORATORIUM FURANGEWANDTE BIOLOGIE
Re1p-oDerctanol, 33.44.5,5.6,6.7.1,88.99,10,10.10-heptadecafluoroa`DcacpohrndiiangmtaogOnEaCReDprGouidduecltiinoen2T1e1st (21d), Limit Test
Page 16.0150 SPrtoujdeyctN-oN.o. D0R2E0S7A1T91C1K
FHierswtbapopmearance of juvenies was checked daly. The number of young fDiarpshdnaiyaof(mporboidulcatinodn iomf myooubnig) Dwaeprhencioauunntetdhaendenrdecoofrtdehd tfersotmatthoenly perveevreynwtavlaelratrielnyeowfalthaentdesntottedma.ilyAfatserrceocuonmtimnegntdheedyionutnhgegDuaipdhelniinae to were discarded. Aobnonrmadioieipsa(ree.lgn,tDsoawpihmnnmiiang behaviour, number of males and winter gs) were observed and recordedalthe observation days.
`tAhteiahieveendDaopfhtnhiataetstthtotatleltenogtmh ocfonecaecnthraDtaipohnn,isaoavnednbtocdoynwtreoilgahntdof control were determined
Measuring instruments
OpxHyvgaelvne
pOxHi-mMeeetre,riOnxoiLa5b30p,HWLTevWe)l 1 (WTW)
WTeamtpeerrhaatrudrneess +HSpyegcrtoo-pThhoelrommoegtrearp,hC(aLdUaFsF)100 LPG 158
Fluorescence 5. Lave)
of algae
Chiorophyi-Flucrometer (KLeFeLo)
EVALUATION
eOleEctCo(aNdoinogb)served LOEC (Lowest effect ove)
rTehsueltNs.OEThCefLorOtEhCe rceopurlodduncottibonewdaetsedremtienremdidnueed dtoirnecotlsyigfnrfoimcatnhte afnedctthseastohlveenttesctoendtoilmiincdoinccaetnetsraatisoingn.ifTihcaentcfomepraerniscoenboefttwheeecnontthreol vcoenrtsruolss.thThmeitsoclovnecnetnotrnaitriooln.waTsheurseewdafsonrostsattaitsitisctailcaclolmypsairginsiofincsa.nt liifmiftrceonncceentartatthieone.ndTphoeinNtObEetCweweanstrheepsoorltveednatscohnetoclalacnudattehed tested geometric mean exposure concentration. oTbhseertevsetdedtocohnacveentnroatsiiognnioffichanet eofsftecftewmheant wchoimcphatrheedtetsotttheemsoilsvent control oTbhseertevsetdedtocohnacveentarastiginoinfoicanttheeftfeesctt fwehmenatcwohmicpharthee otestthte osmolvisent control
rr --------OR--UNO--ACK---LA--BOR--ATO--RI--UM F--UR --ANG--EWA--NOT--E B--IOL--OGIE----------
Report 1-Decanol, 3.34,4,55,6,6.77,8,8,99,10.10.10-heptadecafluoro-
Page 17 0150
e a`cDcapohrndiiangmtaogOnEaCReDprGoudiudecltiinoen2T1e1st (21d), Limit Teso t BSPrtuoujdteycNteoN.o. cD0R2rE0Se741w191C1K
Statistical significance
Body length Biomass Reference test Software. DETERMINATION OF THE TEST ITEM
dSoatmoprlmiinnagtifoon anaslaymtipclael preparation
sRteapnrdoadrudctdieovniaatitoensaonfdthtehetrcoeeaftfmiecnitengtorouvpasriwaittihonmwoearnevdaeltueersm,ined. rTehleatirvees1u0lttshweesroelveexnptrceosnsterdolaasnpdecrocnetrnotla,ge reduction of reproduction STihgenicfoiecfafnitcideenvtiaotfivoanrsiactoinocnesrnhionugdthheavreepbroeduc<eti2no5n r%,ae were duestinegrmtihneeOdnien cWoamypaArniasloynsiwsitohf tVhaerisaonlcveen(tAcNonOtVroAl)anMudltciopnlterol group. `Comparison Method using BONFERRONI test (Table 1 and 3). m"Tehaesbuordeydl(Teanblgoeft7a).lhl aphids of treatment and controlgroupswas dTehteermmeianneddr(yTawbeliegh7)t,s of al reatment and control groups were: cEaClc:ulaatnedd EoCn-yfvoarltuheesrweiftehre9n5c%e tceomn.fiPdraonbictevianltuerevsalwserweerteaken `farcocmoWrdeiangEr10a(1s98t6a)n,daCrodnfpriodceendcuerient(ervBalRs wEe&rTIeOcTNalPcILulIGaGted1962) ECiuzvalue was determined directly from the results obtained. o"fThJeacnadleclulSactiieontnisfwicerVeercsairornieNdo.ou2t.u0s(iWnigntdhoewsso)ftawnadreSiSgimgamaPlSottat el. 2000 (2000), SPs CORPORATION. `"aTnhaelytteicsaltlyevmerciofniecdebnytrgaatisonchofrotmhaetforgersahpahnydwoiitdh mmeadsisa swpeercterometric: 0de.t6e.c1ti2o.n2(4ma/znd=448613. aTnedst39l5e)mtcroenecetnitmreastiwoinsnwetrheestanuadlyypseerdioind after atinpailcyasteeds.afTehre0caonntrdol4, 8solhvinenotnecornotprioclataen.dTshtoecokvseorlaultiroencwoevreery rates `wesreed<o8n0t%he rgeeolmaettertodictmheenaonmienxaploscuornceenctornacteinotnr.atAillonre(sfuorltdsetaariels apllteearnsaetirve,ettfhoepeiamgree w3e2ig(hetqeuadtimoena1n) maenadspuargeed4c0onrceesnutlrtat)i.onAwsasa.n 4ca0lc(urleastutesd)(,for deta please refer 0 page 32 (equation 2.3) and page
A2t4sinxdwa4l8ehr, renewal intervals samples wore prepared ater 0,6, 12, wFiotrhosuatmpdlaipnhgniacfstwee6r,re12p,re2p4aarnedd a48ndhivnceuebaatdeddiItkoenatlhetotesstbebaekaekresrs sAedldeicttoendabyl,usbiefnograe trhaensdtoumdygesntaerrtast3orre(pElXicCaEtLe)s wfoerresarmapnldiongmloyf od (48 p1)retpeasrtastoilountitohnessoeustaomfptlh estwesetrcehsatmobreodrsin(aviftrheedzaeprh,ric). After
_--
OR.UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
1Re-pDoerctanol. 3.344,55,6,6.7,7.8,89.9.10,10,10-hoptadecafluoro-
Page 18 of 50
aD--cac_po--hrndiiangmatogOnaECRDeprGoudiudecltiinoen2T1e1st (21 d, Limit Test
SSPtruoudjoyc-tNe:oN.o. D0RR2Ee0B7s4119i1C1Km
VALIDITY CRITERIA
TDEESVITAGTUIIODNESLFINREOM THE SDETVUIDAYTIPOLNASNFROM THE REPORTING ARCHIVING
pAlalnssa.mples were mainainod as separate replicates. For details see p`Sraemppalrees wfoerrtehdeiarnecatllyytigciavlednetienrtmifnoartti-omnetihmym-ebduitaytloyth.er (MTBE) and iAtmhmreedeiaotfteholryssitxosreadmp(cilnogseidn,teinravalfsresezear) umnwiepraneaallnyaseelsy.sseAdt the other itfhnreeceasinstaerryv.als the samples were stored ina freezer and only analysed, For details soo part, + Meoxrctealeidty2o0f%thealptahreenotnadnoifmathleintestth.e control group should not + sTuhrevimvienagnatntuhmeebenrdooffvthe otfefsstprsihnogulpdrobdouc=e6d0pienrtphaerceonntlraonlimgarloups. + Tofhfespcriongepfrofduiocfcevdairpieeartnipotanreanrtouinndthtehceomnteraolngnrouupmshobofuledinrbge
25%,
None
None.
Trehceorfdin(a1 roeripgoirntas)issuedas paper copy (5 originals) and an electronic
"paTelhrleiofrdoalwsopdewaciitnfagiewdilinbtehreeotpaeirnaetdiivnetnhaetiaorncahlivGeLoPf rtehgeuleasttiofnasc:ility for the. fsitnuadlyrepploarnt((OOrriiggiinna1l1 1125)) ailncrleucdionrgdsmapsetrefrorsmcahdedbuyitehse quay assurance programme + samples of test and reference toms. VMioclrkosfbiamnskwSialrsbteedtr,etaDi-n3e1d1i5n7aSsaarfstee-ddte.posit by
LA oeOLnner3e34e4r5o56m8v7170o8hm,8ata3mto0caAt1un0ms- moeonoPmsaerotsee remeoROGuesheeast ewn
4 Results
ot swing e esl tsing OSEy CoD2m0a2r1t5h8,,T frtecaE ralreesaishteseontsn
Tee. rmmtn test reAritstnonson
|
caEnnonE|
IMMOBILISATION [%)
oHpEer AAC |p CO EeEaIES]
| conta
of o
rw constr
artesdsrwi anton eon earssinebnao ek yg
S ermmt----e --------------a--S--mat--ep _t) A 8
ts dn nr ghconto ws aat,LSerCsargomascotfbhntwsafmemise
Tm Treoewetrmet sptaong eraescesamhaoeatntpte8t gcesrny rs etevoesntstsbtoeneensnees
Ji meteors nt
woa otes orooeressiSamaardt eavseistt 0osevonensotrveestsrmoter
enoneet 8 rss eisss coco rnsoe
DRUNOACK-LABORATORIUM FUR ANGEWANDTE SI0LOGIE
DDReapapohrantina ,mag3n.a34R4e,p5r.o5d6u.c6i1o.7n,T8e8s.t9(.2110).,10.L1i0m-ihTeepsttadecafluoro-
Page 200150 Projecto, 020715K
R ace cordB ing to OECD Guideline 211 ee SStEudyE-No,. ODRREES4M111
42 Results ofthe Definitive Test 421 ReproductiveOutput tvTehasteryGnauwymabtaerercrrsoohfnoejwwvanle.inniRtesasluielnstas8c-r1oe0np.cleirciansgohfeconnturoml,besrovofenktvecnoinetsolofanadl toesst garnodupcsownansrroecvoprsdefdoratavery fbTeehmteunecueomnnbcteehrneot1fa0tuovnaen(diFie2g1surpear2o)y.duwceerdo ian d1d0edreopitcahletsotoafl tthuemcovoenrt,(Tsoalv3eb)ntlacnoodntrpollotaendd vteesrtsgeromunpsfost
i7T2n.h4tehemjeuGvaMennEilnCeus.imntb(hTeaeobrflceoonf3tf)rsoplrignrgouppr,od0u.c6e0d5pearvpaeroanntdainmimmaabseu)rviinvitnhgeastotlhvenetncdonotfrotlhegrtoesutpcaanme16fo67.3
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ol | cons sontcol | 452 lL 1 geometic mean exposursconcntaion [gai]
Flue: Course of Total NuofmHatcbhedJouvenrile after 21 Days
-- -------- ------------
T OR.UNOACKLT ASORATORIUM FUR ANGEWANDT TE B0LOGIE
CRDoapoperci emag1n3a,4R4p.i8o6d6ic6i1o7n88T5o.k1(03.1190,,1L0k-mpTtoaucecaluoroaccordinga OECDGuan211
Page 21 or50 StPuryoNco,. D0R2E0R7A1I01C1K
idiotic..ic. o
Tie3 Mumbr of ovens in th Gol, Sent Contl and est Groups er 21 Days
I
mec| Momr n no epcat oto. |ra
No.
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[3Js - 1 2 oo 70 0os sofsos]
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|
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[oorimey oon nw woo elvl e [ool
0L3E0 -- emnoet Sar tn Tr r m Stiotnusn sttosimee AOOUWBBsomrtron<2005 rcToihnntocalosrhfituonhtccoafmroaoiufo0ni7o.rfeo1vu5ann%diattonde nmeehancsnoounemscobhfooenidtnrbgroofur5i160n4g1r0tu(ebTsaeor3,paTrehntvhyi cr0Thh0aea0ncmWen:aayncTAhnhronowuaamoosamoffVasiaaarbtaisilastneececaslo(yarAmshNocOnVgasAhnu)eitannvdageBroscnaeaanEnpRuRiopno<sfah0.w0e8cr)eoahgrhesatcesorstrarrvawcnwolasodslobcoeronoompcuaoarmsaedhtobyTbhye 1Co0mp1ar0edcohneccaonnvr.aNoonS4t5s31i3c..l.swhceaconmpaarrese1n0hwecsosntoe aonthdSi oevoenmt cronoe
--
LCwooorteAnAAeScATsoSAwP onaasRtaacaweEsop0sve weoI tg| eOiGOGsfnwt--eom-- ter
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---- OFLU NOACKLABORATORIM FUR ANGEWAeNDrTE BOLOGIE
DRDaepopoicrratinaolm,ag3n,a44R5e56p6r7.o7d.8T.o9t9.2110.)10L10theTpetmadecafvoro-
Page zits Projecto. 0207150
A e Scorn 1 OECDA Gude 211ee SSaRye, EORHEBHA11)1
424FirstAppeaorfaovnecnes Tho fst day of appsrance o venisinchial group s given in ttle.
Tibi Fast Appearanceofdenies nh nis Groups [Goamro mrem]| Omar pReepasrarsidwn ropesnce TeGsoal r 1 2 3eae e5 s6 e7e eooetn s wee
sovcenoiGoonra [989 98 93 55 9 9 9 9 5 50 9o]f5oo
425 mmobiksaoifohne Parent Nroamoyraolfltohyfshpeaprareenctsaamce0c1u0%at ns col and sovont contol A te i concenaton
42:6 Growth (Total Lanth and ry Weightofthe Parent Animals iSAonpnipeisectaahnretGaeonvficahellnpsawnrerortfeaoglaoaclhiddlsscwpoamspaavnridicsanolnaoccoyhmopacrgon f0ol iahnd)scoenntGotoavcnoednrsvlocgnrreacssooAmoih:gervoeupne.!No Con and mk concen wore dekrmined (Te 5) Tab Toa Length and Dry Weight of he Pre Avie
| GmEC TotaLonginoRfephsecPaaireetnot.Animals fr] W | | On Wa| ge)
[moins] + 2 8 4587 ss nom sw
Sso5on5rt |[es]455
a~[ss0[s0[]4vass] a45|as4as5[sa[04ss]]]a0s0s]]]aa400s2s[0645]| oa4s7s |
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WRCet c ~CReotn onnitneordeerrcan
-
DR UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
1Re-pDoerctanol, 3.34,4,556,6.7,7,8,89.9,10,10,10-hoptadecafluoroa`cDcapohrndiiangmtaogOnEaCReDpGruoidduecltiinoen2T1e1st (21 d, Limit Test
Page 24 of 50 SPrtoujdeycNtoN.o.D0R2E0B7A1191C1K
427 Presence of Males. No males were present neither in the control nor in the fost groups duringth test.
428 Occurrence of Ephippia (Winter Eggs) No ephippia occurred in control o tos groups.
423 Water Parameters
cToanbcleent1r1atsihoonwns tohneemreeaplsiucrateedpiernatlreaatnmefnt igproHnupv.aalules of the control, solvent control and the mit
"DTehveiantiiaolnspo-fv>al1u.5esunviatrsiiendnftoaml a8n.d i0tnoa08.l13pfHovralutehse caornetrroelg,afrrdoemd7a.s96betio8ng.1si2gnfiofrictahnet,solvent control "aTnhde ffionaml p7-.9v3a1l0ue8s.0v4afroiedthreommit7.c3o3nc10en8t.r6a2tifoornthe conirl, fom 6.97 107.66 for the solvent control Aalnlddefvoiamti6on.s9o12f0 n7i.a7l0faonrdthfienamliptH-cvoancleunetsrwaetiroen< 1.6 units. Tho pH-values wero alin a neutral range,
cTohnetofilnaalnodxyligmeitnccoonncceennttrraattiioonnwseorfethfeoumnedatsourbeed=r7e.p6l3icmagte1sLin(Ttahbeleto1s2t).meTdiha tooftatlhehacrocnnters,s wsoalsvent
1do0t2o2r.m0inCed(T1abbloe
> 140 14)
mL
(Table
13).
The
temperature
in
the
test
vessels
was
within
the
range
20.1
4210 Acute immobilisation Testofthe Reference tem dTehteerpmeirnceedntdaigreectilmymforboimlttyhefotrestthererseuflesr.enTcheefEtCom1;w-aasnddEeCtermivnaeldueastweirh2495h. %ThceonEfiCd-evnaceluinetewravasl. (C1) EwCe-revdaeltueesrmaifnteerdi2n4ha porfotbhaebirletfyerneentcweortkebmyiinnmteLrp:olation accordingto standard procedures.
EECC; 0:81489(CI118-187) EC 1580 cTehnetrEatCion vraalnugeeooffr1e.f0e.re2n.c5emlolalm. opfotqausasliiumcrditiecrhiaroamcactoerdaifntget2o4AQiSs: wDitIhNinGuthiedspirnesec3ri6b4e1d2cLon3-0.
4211 Conclusions cTohnecennotraotbisoenrv45e.3dyefgfecat.c.o.ncentration (NOEC) was determined to be the geomalric mean exposure
DR UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
Report 1`D-aDpehcnainaolm,ag3,n3a4.R4e,p5r5o6d,u6c.t7i,o7n,8T,e8s.t9(.291,100},,1L0i,mi1t0-Theospttadecafluoroaccorting to OECD Guideline 211
Page 25 of 50 PSrtoujdeycNtoN.o.D0R2E0B7A1T9IC1K
5 Validity Criteria + Mortalty in the control and solvent control group did not excoed 20 % at the end of the tes. + The mean number of ing offspring produced pr parent animal surviving at the end of the test was> 60 in the control group. + The coefcient of variation around the mean number of ing offspring produced per parent in the control groups was < 25 %.
6 Literature / References
AVeQrSd-uMnenruknbglsastt:ufBeenst(iDmINmu3n8g41de2r-nLic0h)t,akut gifigen Wirkung von Abwassar gegeniber Daphien ber VBeRrElaTgI.GJGo.n8.TOUPLING W. (1982): Mathoden dor Wassoruniersuchungen Bl, VEB Gustav Fischer uDnIdN i3n8e4a0r2eTKeaiilb5r1ie(f1u8n8i6)t:ioKnaflobrriGoeruBnegstvionmmAunnalgysvaonnvVeerrffaharhevno,nsAkuesnwnegrrtoufinagnvon Anaysenergebrissen DIN 38.412Teil 11 (1982): Bestimmung der Wirkung von Wasserinhalsstofen auf Kieinkrabse ELENOT B.P(1990): Selenium Deficiency n Crustacea, Protoplasma 154, 25 - 30 OECD Guideline 211 for Testing of Chamicals (1998): Daphnia magna STRAUS, Reproduction Test ``GOrEoCwDthGiunihdibeiltiinoen 2te2s1t for Testing of Chemicals (revised draft document October 2000) Lomna sp. `TeOsEtCiDngSoefriDeisfofincultTeSsutbisntgaanncdesAsansdesMisxmtuernes#t. 23 (2000): Guidance Documenotn Aquaic Toxicty WEBER. (1986): Grund, der biclogischen Statist. 0. Aufage
---- ---- -- EE------------
7 Observations at Test and Control Groups
|= BEREEERERLLLELEEEERE oa II Repieaoto. |
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OR UTNOATGK-TLABORATORILM FUR ANGEWANDTEeBIe0LeOGIE
oDReoppcoaritnolm,ag3n3a,4R4e5p5r6o6d1u.c7i.o8n8T.e9s8h.(130,11d0.L1i0nhkeTpotsatdecafluoro-
Pagez7or50 Prjecttio. 0207100K
according lo OECD Gudeina 211 eo
VSto udy No.mDRESAT11
Take. ObsasttvheSoalventtCointnGrsoup
seasonJalaofoa[o[of0oofesa[0ooo]ooaf] so| IL TolTeo Ld Tr [owenm Day [repcaono
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_
DRUNOACK-LASORATORIUM FUR ANGEWANDTE SI0LOGIE
`-RDeDapepochrantinaolm,ag3n3.a4R4e,p5r,o5d6u,c6t7io,n7,T8e8st99(.21104.,10L,i1m0hTeepsttadecafluoro-
Page28.0150 Projecto. 020719CK
a--c_co--rdingto OEe CD Guidee line211
sStuudyNeo.oDRReEBs1e11n
Table 10. Observations atthe Limit Concentration
| eatye rJotflJozorp
a aloo. a10.11.a/12]1E3A] 14.15] 16.
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RC eport OR UNa OACKLABORATORIUM Fe OR ANGEWANDIE 810Lr OGIE Page0cts0
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DR UNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
Re1p-Doerctanol, 3.344,55,6,6.7,7.8,89.9.10,10,10-hoptadecafluoro-
Page 30.0150
`aDcacpohrndiiangmtaogOnEaCRDeprGoudiduecltiinoen2T1e1st (21 a) Limi Test _--
SStPuruodjyecNt-oN.o.DD0RR2E0ES741191C1K
9 GC - Analysis of 1-Decanol, 3,3,4,4,5,5,6,6,7,7,8,8,9,9, 10,10,10heptadecafluoro-
9.1 Method DEMTEETRHMOIDNOAFTION
Equipment
A1-Dnecaanollo.yf3vs.a3re.i4o4su.s5c.o5n6c.e6n.t7r7a,t8i.o8n.s9.o9f,10,10,10-heptadecafluoro-were cacparirtiaerdyocuotlvuimangaussicnhgroemxtaetrongarlaspthaynodanrdas.FuDseeldocSiioincawCaPs-SpIeLrf8ormed with a mass selective detector (MS).
A`uGtaossachmrpolmeartograph Detector Softare
CCPT-C38C0o0m,bViarPiAaLn, CTC AnaLyTcs MS, Saturn 2000, Varun Satur GC/MS Workstation 5.51, Varian
Reagents
ort-butyl methyl ether 99.8%, Batch 42337011 31101, Fuca
CONDITIONS OF ANALYSIS Column 1 (Co2 lforsuampmlinngs started 2003-07-14) InIjnejcetcotror temperature TIenjmopceFotrsriosane tvuorleupmreogprogramme
Gari gas Deltoneicstatoiron mode Runtime Retention time.
`1dC.hv0o2mp5amcmk,CLP-OSWI8BLL(VEaERiDa/N)M.S,BaFtcihm: t9h0i1ck6n5e3s1s:,03.02m5,ym 0Fa0ct.o2rF5oumrm,VFF-i5mmsth(icVkaneRss):,0B.a2t5chy: m90(24e08q5,u3i0fvmo .aColluemnn1t)
Spills 250C
(30
psi
pressure
pus)
for
1
min
--Heating rate | Temperature | Holding ime
| rcimin
rc)
min
=10 1 ae 0 Too10
30
20
50
MHeslium 1 mL/min column flow E1l5e.c3t3rmoinnionisation (EI) approx. 4.9 min
-- --------------------
OF UNOAGK LABORATORIFUUMRANGEWANDTE BOOGIE
RDDeopaoprctpa,mog3n3,a4R4.e5p5r,o6d.c6i.1o.n7.T6e8s,0(92.1190),1L0i.n1k0hTeopxtadecatiuoro-
Page 31 of50 Projecto, 020710CK
r accortinlogOECDGudle ne 211 re SStuLdy N,o. OORREBEA11)1
StanoaRD
Testtom vasusedas sander.
STPARENPDAARRADTISON OF PTERSETPASRaAwTpILOeNSOF
PSRAEMPPALREASTOIFONSTOOFCK Soturion
iAnsttoocuk tsoylumioan oif hiehrt,estuttomdof(51c0o0nmcenLtrawtaiosnsp)reapnadreudsed for cabraton For caltraton ange sespart 92.1
sShmuidteert-bbouttyl wmioh ltasestesrtpwpeerro. g4i0vemnL.ionfth5e0 msla.mcpolneiwcaere file no hleosbeota.ndAvtigeoraoduslyonshoafkaenpoar.at6a.ssodt2iumimncorhixctathciSonllehweas tteosot.GAMSa.phTahseeesxetpaacrattoinonprtohceeduerre pinhcalsuedwedaasnfoovcitcehdmeontct factor. wSiahmpeltesuodm tiheystochk srourtiotnsiwianhal1ys3i0s0.mDoiltownersetedpsiaered ste inthfolowianbeg.
Tal 5: luton of Samples of Stock Scution
Sampo0vatume| Ortonotod
oe soon
er Too]
Tr sno io[ n onToo]
SAMPLE STORAGE aI nneceassary. all samples were stood at 20 + 2 C ul start of
METHOD VALIDATION
mGuaasnsiefsic6a9to+n3o8f5+t4h t6et(t2o)m wbaassecdalocnuhlaetextbyrpnelskstranedaarodfs.heThe coatbcrtaotnonanprdoacuacaunrtoatainodntmheisprwienrceiafdoarpnteadfeotmamDiInNat3o2n64o5f. The methodvas previously validated as cescroadi n report DAIBH113
(CCOANLCCEUNLTATRIAOTNIOOFNSMEAN. Atoscaanlscorumatmievaentmweoadsiufrereedntcomnactahnevmaattiiocnasl. procours were chosen
ORUNOACK-LABORATORIUM FUR ANGEWANDTE BI0LOGI
CRDeeapcopritmiamla3g.n4a4R5p5r6o6s7i7c.i8o5n.T1o0t.1(201,61,0-LienpktTaodtecatluoroSecor to ORCD ulnn 211
Page 20150 PSraoyscNo,.o2O0RE7G1A0IC1K1
coGonocmoentaitcomnosm exposure Gceuoamieotni(c1)moa5angaeunesahnanevxb3ofnhcaOtEeCdD cacacorndisngdocument ps
veanconcatoionnt=|a1tLiSlopne2lostclfoogtnco,ne) togteontogecne. tata l-] ]) (1
Tino woghiasmenn
`concentration
oe con 0 okern cFarocmuthaedes messing rvs man values have een
For renewalparads areas and me-weghtmeaadn
`acsondcoenstrrantisonns wOeEreCDcal-cguluattiednfaolNloo.wi2ng21equations (2) and (3)
arog C LnCioConnccO o)--Lcao(nCcoC nc1)
@
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TW oan.TT1oe0taoO0paAernss
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ton Times corn
-_--
ORUNOACK-LABORATORIUM FUR ANGEWANDTE BIOLOGIE
Report 1-Decanol. 3.3.44,55.6,67,7.8,89,9.10,10,10-heptadecafluoro-
Page 330150
a`D_ac--phcnioarmtadogOinEanCRDgeprGoudiduecltiinoen
Test 211
(21
a),
Limit
Tes!
SSPutruoddjyeNcot.o. RD0R2Ee0B74m1191C1K
9.2 Mothod Validation The methoidnweaarstyvalidated and tested with satisfactory rosulswithrespectto
+ determination mit and detection mit
92.1 Linearity 3T3h4e.4a.n5al5y6t.i6c.a7l7s.y8s.t8,e9m.g9a.1v0e,1n0e.a1r0-rheespptaodnesceaiflutohreoa-n. gTehoef c0a.l2ib-r2atmiongciuoLrfve1s-Dacnadncdhlr,omatograms. obtained for standard and test fom are given in Figure 2 - Figure 9.
9.22 QuantificationLimitand Detection Limit "mTehtehloimditdoescqruiabnteidfiicnaDtiIoNn 3(2L6O4G5)"aNnadchiwietiosf- duentdecBteisonti(mLmOuDn)gswgerreenczael-culated using the bind valu. LThoeo)LOD was determined to be 0.009 mg. and therefor the LOQ was 0.027 mg (threefold the
fLr eoeconno 1e244r.5O6R6e.U6N7O.mA7G.K8o8L9A9B.nO1R0A1Tm8O0RhImpUtMaFdmuUcRaAteNiGoErsWsA:NDmTE oB10LsOGIEooPesoostosr0 fri Csatipboo inmcoissti iesain tmiingoo omOenEDGusSPn waorartet:ieoom0e2snR 0e7rgr0t0
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[ry Projecto c207190K
accotroiOnCgO Guana 211
Sty. OREasTT
men on | Tite 17: Gooscuanaattonasdof hae Ac(seaamtgrudaoonseDa.10c7a, 33448567784.
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-- ORU NOACKLABORATORIUM FUR ANGEWANDTE SIOLOGIE
1-`RDDeapapochrantinaolm,ag3n3.a4R4e,p5r.o5d6u,c6t,i7o,n88T.es9t.9(.2110).,10L,i1m0it-hTeopttadecafluoro-
Page 37.150 Projecto. 020719CK
sl accordi ingtod OECDeGuidels ine211NNSotsud.yNo. DRES111
Til17 con: 1C0o.n1c0ehnptrtaatdieocnastolfuohreo-Ac(tsiavmepIlnignrgeddiaeynst1.1D0sc)ancl 33.44.5.56.67.7889.1,
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Page 0150 Projecto, 0207100K
according 0OECDGudelng211
SSwtauyrNto.oOmRenES11
Tae 15 1Go0nc1e0nthoneopf the aAc(tdsiavmaepIcngardcoiuynto1Dr4e1a-6no, 35.445.6677889.0,
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Page 390150 Projecto. c20718CK
N according 10 OECD Giudeii na 21 N Stu.dysNo. i DRESAdTI1
Tale. 10 cont)1C010oeptnaduccoafuahoeeA-cistaimvpaeliinngtgrdcioaoyt11D4n-e1c6a)sne,33.4.4.58.877.888.1,
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OU NOACK-LABORATORIUM FUR ANGEWANOTE SI0LOGIE
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Page 400150 StPurodjyeNcot.o. D0R2E0B7H1T91C1K
Taio 19. GPeepoimadtaiccatMueoraon-Exposurs Concaiaton of 108cano, 33,44.556.677.0895.10.10.10-
----
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Geometric moan exposure concanraton
53
Tas 20: STeipmteaWdeeicagnhitoerdeM:ean Exposure Cancentation of 1-00 33,44.85.6.67.7.6899.101010-
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1R-oDpoecranol 3344.5.667.7,88,99.10.10.10-hoptadecafiuoroacOcaoprhdiinagmtaogOnEaCRDepGruoaddueciinoon2T1e1st (21. Limit Tost
Page 410150 PSrtoujdeycNtoN.o.O0R2E0B7A111910
94 Gatbrtion Curves
250000
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Report 1-Decanol, 3.3,4.4,5,5,6,67.7.8,8,9.9.1010,10-heptadecafluoro`Daphnia magna Reproduction Test (21 d), Limit Test `according to OECD Guideline 211
Page 42.0150 Project:No. 020719CK. `Study-No. DRES4111
120000
-
100000 |
T8000
6000
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=54539.70x - 5614.87 R=09084
18Fest res ------------------
05
1
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Figure 4: Calibration Curve of the Test item (date acquired 2003-07-17)
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Pageddof 50 Projecto. 020719CK
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Page 400150 ProceNo. 0207100
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Introduction
cDOoennpdaMuracirtmceehdnta2,3s,tphe2ec0i0Sau0lr,vwaeaitltelhraenqrcueeaqlauinetsydtsSoatfhmapneldairnDdgespefSufetocyrttioSoefncroSefhtautrhmeyowSfauytrfhAaercerNoeWyawotMuerepxsQitucaeloaimtEynovfBiufroronemnent NcoenwflMueenxciecowiththe San Juan River in the vicinityofthe RG. Hunt aheep rane near Waterflow,
New Mexico Water Quality Standards
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`Sampling Stations
.
Two sampling sites were selected for this sampling effort.
oSfittehe1. HGS.huHmwnaysAheretporyaonjcuh.s upsiscam fiom County Road 6800 (PNM road), located upsveam
Site 2. Shumway Arroyoa State Highway 550 upstream of the San Juan River and downsizeam qoufaltihteyH.aGs.suHraunnctesrheepleipcartaenschfo(rntohtoe:sesasmapmlpelseslacboellleecdteads a"tWastieer2flionwacacboorvdeaSncheuwmiwtahys"heaGreAtPhe)
AWatseurmmqauarlyiotyfdthaetrceoslullesctiesdadsurfoilnlgowhs.e one-day sampling effort are appended to this document. cwSioatnteceer1n.twraSastticrooenoaslo,mffvdeliraoystwshotalhrvides,dshicitognhwsltayistouxeeysngttesi.nmaatMteosds.ttolbnieogthaatbpllpyertowuxeribrmieadtsaecnldyoct1.0oncattua4bi.ni0ec6d0fhoriogtehptreoramnsesocoodnrdes.rThe
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DIAGNOSIS ANDCOMMENTS 3.15.076150
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RabiesRests liom tha Cantre or saps Control and Prevention:
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