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MICROBICS MICROTOX TOXICITY TEST
TEST SUBSTANCE____________________________________________
Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-203FC. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-203FC. Current information indicates it is a mixture of 0.85% PFOS, 17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7% alkyl ether sulfate, 2.55% sodium octyl sulfate, 0.12% sodium nitrite, 0.09% sodium molybdate, 0.85% triethanolamine, and 0.04% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:______________________________________________ _ _ _
Method: Microbics Microtox "BASIC" Procedure GLP: No Year Completed: 1997 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions:
Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A 2 g/L primary stock solution was prepared bj^ dissolving 200 mg of test substance in 100 ml_ of Millipore Milli-Q water. The pH of the stock solution was 6.6. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL of stock solution. Appearance of test solutions was noted as "clear". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss
RESULTS_________________________ :___________________________
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L
001339
Element value:
5-minute EC50 = 604 (572 - 637) mg/L 15-minute EC50 = 444 (423 - 466) mg/L 30-minute EC50 = 363 (349 - 376) mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS____________________________________________
The FC-203FC 30-minute EC50 for Photobacterium phosphoreum was determined to be 360 mg/L with a 95% confidence interval of 350 to 380 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
DATA QUALITY_______________________________________________
Reliability: Klimisch ranking = 2. This study meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES_____________________________________ __________
This study was conducted by the 3M Environmental Laboratory, St. Paul, MN, Lab Request number R1335,1997.
OTHER______________________________________________________
Last changed: 6/26/00.
001340
ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET
TEST SUBSTANCE: f c - Z O f f\F F F
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1. Preparation ofStock Solution
* M ass/Vol TestSubstance:
*
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FinalDilution Volume: Diluent: IVnWW-G)
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* Final Concentration ofStock Solution: 2-<^ \
2. pH Adjustment ofStock Solution
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3. Osmotic Adjustment of Stock Solution
* Osmotic Adjuster:
_____
* Comments: lO r M r ^
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aAcVed
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4. Appearance ofpH and OsmodcaUy Adjusted Stock Solution l^jCLEA^y?r OPAQUE (circle one)
* Color:
C kar
* Comments:
5. Comments
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MCROTBOaXsicD: ATTAestR.EPORT 3 - 17- 97 TRI
Test. Time 5 minut.es
Osinoti o Ad,ius tm ent : NaCi
NUMBER
ru/TT
GONO . CE/GAMMA % EFFECT AV % Q DtFF
CCuotn'st.rr*o'i1 99 86 .. 33 18 // 88 64 .. 36 93
fft).. 0ft (01..88996050 tt
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99899998 93648374 ........ 8693444145672410//////// 66477243 34.U68637........4863202075662925
11225501105500002200000505........0000000U0000000Q0000000ft0000000ft
00000011 ........113783888041560817323500
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9
22446611 64542b23 ........74355983
13.9 25.3
q
1 s-# 1P
43.7 q 0.8
64.8 q 0.5
CttPU=sedCoCf oORr ReEaCLTIoOuNl a EtAi oCTnOsR = 0 . 8 9 3 3
EC50 6 0 3 . 5 mg/L ( 9 b% CONFIDENCE RANCE:5 7 1 . 5 TO 6 3 7 . 3 1
S ig n a tu re CONTINUED TO NEXT PAGE...
TEST TDAIMTEE::_3_^_J____Z__l_fL__?
001342
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! 50 1000 CONCENTRATION 10E4 1OK.5
ESTIMATING EQUATION: LOG C = 0.8450 x LOG b +2.7807 95% CONFIDENCE FACTOR: 1.05599 FOR EC50 COEFFICIENT OF DETERMINATION: F'P. = 0.99676
001343
FILE: R1335.KI5 FC-2U3FC AFFF
MICKOTOX DATA REPORT
B a s ie Tftst
3-17-97 TKS
Test Time : 35 mi nof.-::;
Osmotic Adjustment :NaCl
NUMBER
10/IT
CONG.
CR/GAMMA % EFFECT AV % Q DI FF
Ci;n !,rol Contro]
95.3!/ 79.00 96.36/ 79.3 7
0. 0.
0.8289 tt 0.8289 tt
I
98.44/ 65.75
128.OOOU
0.241
9
93.87/ 65.77
129.0000
0.183
19.4 15.5
17.4 <? 2.0
3
93.44/ 54.00
250.0000
0.4.34 tt
30.3
4
97.31/ 53.96
250.0000
0.495 tt
33.1
31.7 1.4
5
95.16/ 35.55
500.0000
1.219 It
54.9
6
89.96/ 35.23
500.0000
1.117 tt
52.8
53.8 <2 1.1
7
94.42/ 19.73
1000.0000
2.967 tt
74.8
8
84.65/ 17.36
1000.0000
3.042 tt
75.3
75.0 Q 0.2
CR - CoCORRECTION FACTOR = 0.8289 tt Used for calculations
EC50 443. 7 mg/L (95% CONFIDENCE RANGE:422.7 TO 465.71
Signature CONTINUED TO NEXT PAGE...
TEST DATE:
~ f 1
T I M E : __________
001344
FILE:RI 335.Kl6
PAGE 2
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100 1000 CONCENTRATION 10E4. 10E5
bESTIMATING EQUATION: LOG C = 0.7391 x LOG
+2.6471
95% CONFIDENCE FACTOR: 1.04968 FOR EC50
COEFFICIENT OF DETERMINATION : F "2 = 0.99635
00134
F TLE: F1335.K3U FC-203FC AFFF
MICROTOX DATA REPORT Basic Test
3-17 97 TKK
Test Time;: 30 minutes
NUMBER
IO/IT
CONC .
Osmotic Adjus tment:NaCl
CR/GAMMA
Q% EFFECT AV % DIFF.
Control Control
95.31/ 73.01 96.36/ 7.3.74
. . 0 0.0
0.7660 1 0.7653 #
1
98.44/ 60.31
125.0000
0.250 #
20.0
Q2
93.87/ 58.48
125.0000
0.229 #
18.6
19.3 0.7
3
93.44/ 45.74
250.0000
0.564 #
.36.1
i
4
97.31/ 47.30
250.0000
0.575 #
36.5
36.3 9 0.2
5
95.16/ 26.99
500.0000
1.699 #
63.0
6
89.96/ 27.71
500.0000
1.486"#
59.8
61.4 d 1.6
7
94.42/ 14.33
1000.0000
4.045 #
8 0.2
6
84.65/ 12.96
1000.0000
4.001 #
80.0
80.1 <? 0.1
CD - CoCORREOTTON FACTOR = 0.7656 # Used for calculations
EC50 362.6 rng/I. (05% CONFIDENCE RANGE: 349.1 TO 376.6 )
Signature CONTINUED TO NEXT PAGE...
EOT DATE : 3
? ~ *f 7
T I M E : ____________
001346
3 > ;
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FILE:R1335.K30
PAGE 2
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100 CONCENTRATION 1000
10E4
bESTIMATING EQUATION: LOG C = 0.7283 x LOG
+2.5594
95% CONFIDENCE FACTOR: 1.03851 FOR EC50
COEFFICIENT OF DETERMINATION: R '2 = 0.99762
001347
