Document jyBm2O0vNRkJZ8axjYy5jmZq2

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 101. The testsample isFC203CF. Currentinformationindicatesitisa mixtureof 1% PFOS, 20% diethyleneglycolmonobutyl ether,70.2% water,2.75% Acrylicfoamer, 3% sodium octylsulfateI,% triethanolamine2,% sulfonatedalkylethersalts,and 0.05% tolyltriazole. The followingsummaty applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline201 Type: Acute Static GLP: No Year study performed: 1991 Species: Selenastrumcapricomutum Source: In-houseculture.Originallfyrom the CultureCollectioonfAlgae at the UniversitoyfTexas atAustin,Texas. Element basis: Algalcellcounts (cells/mig)r,owth rate Exposure period: 96 hours StatisticaMlethods: Statisticanlalyseswere performed usingthe computer program ofStephan, 1983. Analyticalmonitoring: pH, conductivitya,nd temperature Test Conditions: Algal NutrientMedium: Nutrientmedium per U.S. EPA, 1978. This nutrient medium providedallmineralnutrientsessentialforalgalgrowth and also served as the diluentforallalgaloperations.The pH ofthissyntheticalgal medium was 8.0. Stock and testsolution preparation: A primary 1000 mg/L stock solution was prepared inalgalmedium, and added directltyothe exposure vessels. Exposure vessels: 250 mL Edenmeyer flaskscontaining100 mL test solutionand capped withinvertedglass beakers. Agitation: Continuous Number of replicates:3. Initiaclellloading: 1.0 X 104 cells/mL. Number of concentrations: fiveplusa blank control Lighting:Continuoulsightiantg44 pEin/seC/Mu2sing cool-whitefluorescent lamps. Water Chemistry: pH range (0- 96 hours): 8.0 - 10.8 (controlexposure) 8.2- 8.3(1000 mg/L exposure) Test temperature range (0- 96 hours: 21.9- 22.3'C (incubator) RESULTS Nominal concentrations: Blank control,62.5,125,250, 500, and 1000 mg/L 96-Hr.Algal Growth Response values Nominal Exposure Conc. (mg/L) Control 62.5 125 250 500 1000 Cell-Count (cells/mL) 602,000 451,000 207,000 12,000 0 0 Avg. Specific Growth rate 0.043 0.040 0.032 0.002 0.000 0.000 % Change from Control -7 23 95 >100 >100 Element values: 96-hour ErC50 = 143 (130 - 156) mg/L 96-hour NOEC = 62.5 mg/L Element values are based on nominal concentrations. Reversibilityof Growth Inhibition:Not conducted. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance exhibitsa 96-hourE,C50of 143 mg/L witha 95% confidenceintervaolf 130 to 156 mg/L. The 96-hour No Observed Effect Concentration(NOEC) was 62.5 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY I Reliability: Klimisch ranking = 2. The study meets the criteria for quality testing.However, the sample puritwyas notpropedycharacterizeadnd the study lacksanalyticaclonfirmatioonfthe amount offluorochemicaplroportion inthe solution. REFERENCES Thistestwas conducted by EnviroSystemsDivisionR,esource Analysts, Incorporatedo,f Hampton, NH attherequestofthe3M Company, St.Paul, MN, Lab Request number J1884-3,1991. OTHER Last changed: 6/28/00 Study Title Static Acute Toxicity of YC-203CY to the Alga, Solonamo, aWAcornut= AMIlk2U Timothy J. Ward Robert L. ft&rl Study ...@l.ted August, Performi;@ Resource Analystsi,-,Xxncorpora One lafayfttii-Rmd sampton. xw Rai4ohLre 3"3 -7-' I summty The acute toxicity of FC-203CT to the alga, solonastxlm capricornatum, Le described in this flnal r"mrt. The tout was conducted for 3H Oompany for 96 hours during July IS to 22, 1991, at the ZnvLroSystma Division of Resource Analysts, Inc. in Hampton, new Hampshix,e. It was conducted by Joamn Magazu, Peter Kowalski, Zll*n Stanford, Robert BoorL, and Timothy Ward. The test was performed under statle conditions with five concentrations of test substance and a dilution water control at a man temperature of 22.1*C. The dUutLan water was synthetic @dL&.Preparod with sterile doLonL&od water. Flasks were incubated on a rotary shaker. Light was adjusted to 44 uZLn/sec/a2 with a phatoperlod of 24 hours light and 0 hours dark. Nominal concentrations of rC-203CF war*: 0 mg/L (control), 62.S mg/L, 12S mg/L. 2SO mg/L, 500 mg/L, and 1,000 mg/L. Nominal concentrations were used for all calculations. Algae us" in the test was from an in-house culture that was maintained under test conditions. txposure of alga* to the test substance resulted in a 72 hour SCSO of 230 mg/L FC-203cr and a no observed effect concentration (WORC) of 62.5 mg/L. The 96 hour MCSO and ROBC are 143 mg/L and 62.S mg/L, respectively. r 4-, "ai,@Page 2-of IS, It. Tua or cm&=,L summary Tabls Of COntOnts Index of Tables IV. Introduction V. Methods and Materials Vi. Results viz. References appendix A.',water Quality Data frm TeoiLcityTest PAGE 2 3 4 5 5 8 12, 13 3 ommuce Ill. INDZX Of TABLU Table 1. Chemical characterization of a representative sample of doLonLzod water used to prepare test media for toxicity test Table 2. Growth data from toxicity test Table 3. Percent change from control and average specific growth rate from toxicity test Table 4. Median effective concentrations (RCSO&I from toxicity test Table A.I. Temperature measured during toxicity test Table A.2. pH measured during toxicity test PAGZ 6 9 20 11 14 is Xr, @ke @4,'4 t IV. irrYA)Ducnm This study was sponsored by 3M Company* St. Paul, Minnesota. The objective of the study was to dateralm the &cuts toxicity of rc-203CY to a freshwater alga. The v-y-rt contalne sections that describe the @thods and materials employed in the, *tuft, and the results of the investigation. The report also aantalue an appendix that presents the water quality data collected during the test. V. XBTHMS AND XRTMIJA TEST SUBSTANCZ: FC-203CF (ZnvLroSyst@ Sample Numimr 2937Z) was delivered to ZnvLroSysteme on May 23, 1991. It was contained in a 4 as. plastic bottle that was labelled with the following Lnformationt "JI884-3# FC-203CF"o FC-203CP (an orange-brown liquid) was -Vyliod by 3H Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to mm the test material was stored in the dark at room temperature. A reserve sample (approximately 2.0 gr=) will be retained at gnvLroSysteme for a -Ini-- of 10 years. DILUTION WATZRI Water used for acclimation of test organl@ and testing was sterLIe enriched media (U.S. ZPA, 1978) approximately 8.0. Results of ch@4c&l analysis of sample of deLonLzed water used to prepare the media Table 1. for all toxicity with a pH of a representative are presented in TEST ORGAMISK: Algae' used for the test was frm a culture originally procured from the Culture Collection of Algae at,the University of Tozan at Austin and delivered to MwLrosystems on May 14,'1992'.",The identity of the culturs, was verified using., an, appropclato taxonomic @ )my. The culture was:*'*'' transferred to sterLIe. enriched media' identical to media used for this test and maintained at,,,tint'conditLcmm Tmxci TY TZSTINGs BCC"N 2,o8r JL9 1, 000 conc*sit'rat greater og;(L the 1%71@l,@.Lth,th-etamt-,substance ip-- conduct' 24' -'was 1*88 7nii6@er;,@of ter 96: bmro the@:iii6e@-a -COIL a pe thazi;,t the controgand at COX" cot o'lipea54--ow,-,W''@. so a f;.thgi'ccithi or,-* 199i Tho," defLnLtL"@'@'.toxLaLty@! t' -It Le based on procedures of,,. was Porformd dur '01=@ (21)84). July,15.,to,, PAK 5 of Is Table 1. Chemical charActOCLIation of a rVreoentatLve sample of doLonLzed water used to prepare test media for toxicitytoot Paramter OrganochlorLne pesticides Unit of Heasuremnt ug/L Reporting Limit 2 Measured Value RD Organophosphorus ug/L 0.5 ND pesticides PolychlorLnated ug/L 0.5 ND biphonyls Note: ND not detected at or above the reporting 11-it. ce The test was conducted at a target t ature of 22 1 2*C with five concentrations of test substance.and a dilution water control. A 1,000 mg/L stock solution was prepared Ln dilution water. The stock solution was added directly to dilution water contained in the toot vessels without the use of a solvent. goolnal concentrations of the test material worst 0 mg/L (control), 62.5 mg/L, 125 mg/L, 250 mg/L, SOO mg/L, and 1,000 mg/L. Algae was randomly and equally distributed among three replicates of each treatment at the rate of 10,000 cells/ml. The toot was performed in 250 ml glass Zrl*nm"r flasks that conta!.ned 100 ml of test solution. The flasks were capped with inverted glass beakers. Test vessels were randomly arranged on a rotary shaker in an incubator during the 96 hour test (a random numbers table was us" to select the location of each vessel). A 24 hour light and 0 hour dark photoperlod was maintained with cool-whlto fluorescent light@ that provided a light intensity of 44 ule-Im-2 The number of algal callo/=I in each test vessel was determined visually every 24 hours by mans of direct microscopic counts with a hemacytometer. The pH (Beckman model pEl 51 motor) was determined in each test flask at the beginning and and of the test, and temperature (ASTH mercury thermometer) was measured and recorded daily in the incubator. STATISTICRL METHODST Results of the toxicity test ware interpreted by standard statistical techniques (Stephan, 1983). The binomial or moving average method was used by the author to calculate ECSO values using nominal concentrations of test substance.@@.''@T,he.@,-'N' was calculated using Dunnott*s test, which includes a. parametric ora-tmy analysis of .,'.varLanco (ANOV-IL). The average specLfi6'growtb rat* was calculated as the' natural log of the number --of@colls'per od at 24, 48, 72,-,cc 964 hours minus the divided by, the natural sure log of tliol"iimb@r@ofcells per al at 0 hour s ............ period -4f The@ percent 'change from control 'i calculated I by subtracting t" -&-v'e'ra"s-pecific growth raLt@4!fz;ca ,,t" control' average speeLfLc,,, rit@i,'.dLvLdLng that..value ',"average specific growth rate' thi@:-control &ad multiplying 100. The percent change:from contro -its&u,s" to@ to Z, values.., 4. Pave.,7 of ipm, Vi. RIMMTS Biological and water quality data- generated by the acute toxicity test are presented in Table 2 and Appendix A, respectively, and the percent of control and average specific growth rate information is prommt&d in Table 3. The 24, 48, 72, and 96 hour BC50a for alga* exposed to FC-203CP are presented in Table 4. The 72 boar ECSO in 130 mg/L, and the 72 hour MORC is 62.5 mg/L PC-203C]F. The 96 hour ZC50 in 143 mg/L and the 96 hour IIOZC in 62.5 mg/L. ID Tal@le 2. Oxcwth data from toxLcLty test NooLnal concentr&tlon img/L) rep. 0.0 (control) 62.S 125 250 500 irooo 1 2 3 mean 1 2 3 mean. 1 2 3 mean 1 2 3 mean 1 2 3 mean I 2 3 me an I mu mber of calls per MilILILter x 103 (hour) 0 24 48 72 96 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 lo:. 10,. 10 28 28 38 31 20 22 22 21 20 is 22 20 16 22 is 19 12 20 16 26 10 10 10 -'.10 128 116 146 130 68 44 66 66 44 so so 61 14 20 20 is 6 26 4 9 4 22 4 7 310246 288 282 218 162 156 179 S2 so 74 61 12 12 10 11 4 2@ .4 .3 .4 620 512 674 602 470 386 494 451 220 198 202 207 26 12 a 12 0 0 0 0, o, 0 0 pa" 9@of --A* Table 3. Percent change from control and average op*cLfLe grow b rate fi@om taxLcLty test noolnal Concentration of Test Substance (mg/L) Average spec!!Lc growth rate 24hr 48hr 72hr 96hr 0.0 (control) 0.048 0.053 0.046 0.043 62.5 0.031 0.040 0.040 0.040 125 0.029 0.038 0.025 0.032 250 0.027 0.012 0.001 0.002 Soo 0.020 -0.002 -0.016 0.000 1,000 0.000 -0.009 -0.009 0.000 Percent change from control 24hr 48hr 72hr 96hr 0 0 0 0 35 24 13 7 40 28 46 23 44 77 98 95 58 >100 >100 >100 100 >100 >200 >100 Table 4.1 M*dLan of feftive 00roftatc&tLOns (BCSCB)tem toxicity test axposure period Rcso 95 percent confido" limits Calculation nothod 24 hours. 48 hour@ 72 hours 96 hours 212 mg/L 156 mg/L 130 mg/L 143 mg/L 182 - 244 mg/L 140 - 174 mg/L 120 141 mg/L 130 156 mg/L Moving Average Having Average Moving Average moving Average 7 'of is Viz. 08=. 1984. Guidelinesfor Testing of Chmicals. Section 2 t If feats on Biotic Systems. Method 201, Al@a, Grow h Inhibition Test. Adopted Ji, 4, 1984. Stephan, C.S. 1983. Comput*r program for calculation of LCSO values. Personal communication. U.S. RPA. 1978. The Solonastrm caprlco=u.-un Bottle Test. ZPA-600/9-78-028. July, 1978. PrLntz Algal Assay '7 IP 12'oo Aippendix A. WATER OUALITY DATA FROM TOXICRTY-M= Table'A.l. -T- Day of gxpoouro 2 3 4 toxielty t*st Temperature of Incubator,*C 21.9 22.1 22.1 22.0 22.3 '4@ Table A.2. pH wasured during toxicity test W=Lnal concentration (mg/L) 0 (control) 62.5 125 2SO 500 1, 000 Replicate 1 2 3 1 2 3 1 2 3 I 2 3 1 2 3 1 2 3 PH initial final 8.0 10.8 8.0 10.8 8.1 10.6 8.2 10.4 8.2 10.2 8.3 20.2 8.3 9.0 8.3 9.0 8.3 9.0 a.3 B.S 8.3 8.5 8.3 8.5 8.3 8.4 8.3 8.4 8.3 8.4 8.2 -8.3 e.2 8.3 6.2 8.3 14