Document jy7knpOMJrBrdaReJLwEMdMB5
AR 26-0537
TOXICITY TO AQUATIC PLANTS (E.G., ALGAE)
TT ESTE SUBSs TANCT E Sue0s 0y 00 ANCE Identity: Perfluorooctylsulfonate, didecyldimethylammonium salt; may
also be referred to as Fluoroalkyl ammonium derivative. Decaminium, N-decyl-N,N-dimethyl-, salt with
1,1,2.2,33,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-1octanesulfonic acid (1:1), CAS # 251099-16-8)
Remarks: The 3M production lot number was Lot 1. The test sample is
L-14394 referred to by the test laboratory as P3025. The sample was
labeled F-11615, Lot 1. The test sample is a mixture of the test substance
ionf wraestiedrua(lappperrofxiuiomraotcehleymi3c0a-ls4)0.% tAellstvsaulbusetsarnecpeo,rt6e0d-r7e0l%atewa1t0ert,hisanmidx0tu-r5e%.
oTphaeqtueestssolaimdp)lwehiacphperaarpisdltyo were made to adjust for the
sbeepaar2a-tpeshaasfetedriasgpietrastiioonn. (cNloearcallicquulidatwiiotnhs actual concentrationof the test substance
in
the test sample.
METHOD
Method: OECD 201 Test: Static acute GLP: No
Date exposure completed: 1/11/97 (study repeated on 1/13/97; second study exposure completed 1/17/97)
SSpoeucricees:: OrSiegliennalalsytfrruommcTaphreicAomrenruitcuamn Type Culture Collection (ATCC),
Strain 22662. Analytical monitoring: Nominal concentrations. PH, conductivity, and
tEelmepmeernattubraesiwse:reGmroonwitthorreadt.e and number of cells/mL.
Exposure period: 96-hours
Start date: 1/7/97
TEensdt doartge:ani1/s1m1s/9l7aboratory culture: Algae cultures were growing in 4OEdCaDys-rbeefcoormemteesntdienditicautilotnu.re medium at AScl Corporation, Duluth, MN for
Test Conditions:
Test temperature range: 25.6 -- 26.0C
04267
rDielcuotmiomnewnadteedrcsoonucrecnet:ratTihoensalbgyalspmiekidnigudmeiwoansizperdewpaatreerd wtiotOh ECD
nviuatr0i.en2t2 sptmocfkislt(raitnicolnupdirnig oNtroa;uEsDeTAin).theTtheset.meTdhieumpHwaofstshteersiylnitzhedetic
aSltgoalckmaenddiutmesattstoelsuttiinoinlaptiroenpawraasti7o.n8:9.Water accommodated
fbryacmtiaosnss. adTdeisttiosnoloufttieosntsswuebrsetapnrceepairne5d00formeLacofhatlegsatlcmoendcieuntmr.atTihoen
tweasst sduebtsetramninceedw.aTshtehosrooluugtiholnyshwoemroegevingiozreodusbleyftorieeedacfhorm2a0s-hsours
(pvhoarsteexw1a/s2 --pi1p/e3ttseodluftrioomn tdheeptvhe)s.seTlhseafutpepretrhelasyoelrutofiotnhsehaaqduesoeutstl.ed
for 1-hour. The concentrations,
test solution in the highest two 24 and 40 mg/L were a cloudy
exposure milkish color,
while
tmhge/L14c.o4ncmegn/tLraatnidon5.w2asmgs/liLghctolnyccelnoturdayt.ioAnswwhietree
clear, and precipitate
the 8.6 was.
also observed on the concentrations during
bottom of the flasks WAF preparation.
a
all
loading
Eplxupgogseudrweitvhesfsoealms:stSotpepreirlse 2co5n0tamiLnibnogro1s0i0limcaLteofErtlesetnmsoelyuetriofnl.asks
Agitation: Number of
Shaken continuously at 100 replicates: Six for control,
rpm three
per
test
substance
loading
Initial algal
Number of
ccoenllcelnotardaitnigo:ns1:.0
X 10 cells/mL
Five plus a negative
control
Water chemistry:
PH range (0 - 96 hours)
7.89-9.84 813-932
(control exposure) (40 mg/L exposure)
Conductivity range (0 - 96 131- 150 imhos/cm
hours) (control
exposure)
Test te1m3p4er- a1t5u6reumrhaonsg/ec(m0 (--4096mgho/uLres)xposure)
25.6 -26.0C
Light levels: (0 - 96 hours)
730-750 lighting
ft-c
from
continuous
cool-white
fluorescent
cRoenmcaerntkrsa:tioInnscownasisstseenetn,grpoawrttihcuilnahrilbyitaitontahte i8n.c6rmegaisLi_ngexepxopsousruere
concentration. were less than
As the
alorweessultt,extpheos4u8reancdon7c2e-nhtorautriEonL5(05.2bamsge/Ld),onwhciellle
growth the 48,
7e2x,poasnudre96c-ohnocuerntErLa5ti0onba(s4e0dmgo/nL)g.roTwhthe rNaOteEwLer(ceelglrdeeantseirtty)hawnatshealhsioghest
lower than the lowest exposure concentration.
04288
RESULTS
Nominal concentrations: Element value:
Bk
control,
5.2,
8.6,
14.4,
24,
40
mgiL.
2244--hhoouurr EErLLso((cgelrlowdetnhsriattye))== 1155..89((1134..24-~171.86.)8)mgm/gL/.L
48-hour EL (cell density) = <5.2 mg/L (CI not calculable)
4782--hhoouurr EE/LLso ((ceglrlodwetnhsirattye))==<>54.02 mmgg//LL ((CCII nnoott ccaallccuullaabbllee))
72-hour 96-hour
E.Lsy EL
((cgelrlowdetnhsirtayte))==2>04.08
mg/L (16.4
(Cl not -- 26.4)
calculable) mg/L
96-hour 96-hour
E-Lsy (growth rate) = NOEL (cell density):
>40 1.9
mg/L. (1.6 --
(CI not calculable) 2.5) mg/L"
"96N-OhEouLrvNalOuEesCa(rgeraowptphrorxaitem)a:ti1o8n.s9
(14.6 using
-- 22.1) the IC
mg/L" as the
endpoint.
All element values based on nominal concentrations.
Svtaaltuiesstiwcearlemecatlhcoudlast:edCuelslindgenasnitiEexsc,elgrsopwrtehadrsahteeest.anTdhpeerTcreinmtmiendhibition S(Vpeerasrimoann2-.K01a,rbUeSrEmPeAt-hEoRdL,anDdultuhteh,InhMiNbi)tiwoenrCeonucseendtrfaotriEoLn a(InCdp)NaOpEpLroach determinations,
Control response: satisfactory
Biological observations after 96-hours:
CancaoTneutsstion, [o[fmlCalls par |iDbennyvia GnroowthnRavtiea
[s5 cm [oomon|.| | [oe Tiwm|m 5| [ee Tomo | ww | & | [[ooee T To o||mwm oo0o ||o w||
C Tommo [e | 0]
Observations: meansof direct
Algal cell counts in microscopic counts
each test vessel were determined with an Improved Newbauer
by
hemacytometer.
047269
CONCLUSIONS Tdehteertemsitnsedamupslieng96t-whoocuarlcEulLatiionntemrevtalhofdors.SelBeynacesltlrduemnsciatyp,riitcowmansut2u0m.8was
mg/L with a 85% confidence interval of 16.4 -- 26.4 mg/L, and by growth rate >40 mg/L (CI not calculable). The 96-hour NOEL was determined to be 1.9 mg/L using cell density and 18.9 mg/L. using growth rate.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DDaAtTAAQUuAALMIYTY
0000 00 000 _
not sufficiently characterized. Accurate ELsy's could not be calculated at Reliability: Klimisch ranking 3. The study lacks analytical measurement
of test substance concentrations in the test solutions and sample purity is
each observation period due to the inconsistentdose-response relationship and low inhibition observed at the exposure concentration loadings. Additionally, data is foar mixture and toxicity cannot be positively attributed to didecyldimethylammoniumPerfluorooctylsulfonate salt alone.
preparation. These are: Report quality: Two definitive studies were conducted on this test
substance at the same laboratory, in the same time period. A careful examination of the raw data revealed several errors. during report
1. The study completed" dates printed on the report covers
(eJaacnhuaotrhyer2.9,T1h9e97seacntidonFseburnudaerry"211.,0 1G9e9n7e)raalreInsfwoirtmcahteidonw"iftohr
"Definitive Test Dates" are correct for each report, however. 2. The discussion under section "3.0 Test Methods" for "Test
loadings" are switched. However, the test concentrations listed in this section are correct in each report. 3. The algicidal/algistatic determination test was only conducted after the second study. The discussion under "4.0 Results" in the report with the cover reading January 29, 1997 should not have been included. No evidence was seen in the raw data that
ftihrest asltguidcyi.dalT/haelgirsatawtidcatdaetferrommintahteiosnecwoansdcsotnudduycrteevdeadluerdintghatthe
the test substance was, in fact, algicidal.
REFERENCES
_
This study was conducted at AScl Corporation, EnvironmentalTesting Division, Duluth, MN at the request of the 3M Company.
94270
OTHER Last changed: 5/24/00
04271
EAnSveilr"oCAnSmoeenrSttspudTyoes1rtDAianSge0tl1DDii0vu.iol0sia6nos8n
STUDY TITLE
GROWTH INHIBITION OF _P?<$
TO
GREEN ALGA (Selenastrumcapricornutum)
DATASTANDARD OECD GUIDELINE 201
STUDY COMPLETED February 21, 1997
TESTINGFACILITY.
AScl Corporation/AScl-Duluth
Environmental Testing Division
444D4uluAtihr,paMrkNBou5l5e8v1a1rd
TFealx. NNoo.. ((221188)) 772222--24500420
STUDYIDENTIFICATION NUMBERS ASC Study ID# 5010-068
3M Company Lab Request# P3025
SSppoansnosr: Su3dMy C1o0m4pPa3n0y25
1
0a272
EAnSveiloACnSomrepnSraalyiTona1/n0A7SgeSD0Dou0na0gn
1.0 GENERAL INFORMATION
Study Title
| Sponsor
i
Growth Inhibition of p3e<
Ny
to Green Alga (Selenastrum capricornutum).
OECD Guideline 201
3M Environmental Technology and Safety
Services, Building 2-3-09, 935 Bush Avenue,
St. Paul, MN 55133; Tel No. (612) 778-7452. |
Sponsor's Representative
1 Sponsor's Lab Request?
Susan Beach, 3M EnvironmentalTechnology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133;
[pss Tel No. (612) 778-7452.
i
[T ro bres oone] `Testing Facility Principal Investigator
| Testing Facility Director
AScl Corporation/AScI-Duluth Environmental "Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. "Todd McGuire
Donald Mount
--
Retention of Raw Data and Final Report
L1I Location of Raw Data and Final Report
zy |
Two years from the date study is completed.
TAeSsctlinCgorDpivoirsaitoino,n/4A4S4c4I-ADiurlpuatrhk EBnovuilreovnamred,ntal
Duluth, MN SS811; Tel. No. (218) 722-4040. |
SSppoonmsoarr. Su3dMsCDoompFaancyes
2
04273
EAnSveilroCnmoernpsoarlaiToens/tiAnSgelD-iDviuslitonn 'ASel Su IDF 5010-068
2.0 OBJECTIVE(S)
To determine the 4-d median effect loading (ELse) and the 4-d no observable effect loading
| (NOEL) of the test substance, p3c7v
, for green alga (Selenastrum |
| capricornutum) under static test conditions.
i
This study {preparation
was was
conducted according to OECD water-accommodated fraction
(GWuiAdFe)linpeer2G0i1rl(iOngEC&DWh19a9l3e).19T9e4s.t
solution
Test Substance
|
|
3.0 TEST METHODS
|
gPrraovpietryticeas.:
#3:7< 1.2 (water
=
1),
stable,
and
, two insoluble in
phase water.
liquid,
specific
Test loadings: 0 (algal medium control), 5.2, 8.6, 14,4, 24, and 40 mg/L.
r`Teessutltlsoaodfipnrgevriaonugse dweafsinditeitveermteisntesdinfrwohmicrhanignea-pfpirnodpirnigatteesctornecseunlttsr,atainodn
ranges were substance to
used. Each test 500-ml aliquots
loading of algal
wmaesdipurme.parTeedstbysumbastsasncaeddiwtaison
of
test
thoroughly homogenized before each was vigorously stirred (vortex 1/2 of
mass was determined. Each solution flasks' depth) for 20 hours then
allowed a white
to sete for one hour to precipitate was observed
obtain WAF's. at all loadings,
During WAF preparation, except the 14.4 mg/L.
loading. A second flask loaded at 14.4 mg/L was also set up, and this
second tested,
solution and both
produced produced
the precipitate. essentially the
Both same
14.4 mg/L loadings were results. The presence or
|
tleasctksoufbsptraencciepittaoxtiicointyd.uriTnegstWsAolFutipornespawrearteioonbdtiadinneodtfarpopmeafrlatsoksafbfyect the
pipetting off upper layer aqueous phase.
SSppoonnssoorr: SiIuMdy CIoDmApPa3n0y25
3
C0474
EAnSveilroCnmeontralpToesrtSiancgtDDiiuviolsainosn "ASel Stuy 104 010.048
Test Organisms
|
I Algal Medium
| Exposure Chambers i
I
Observations
| Endpoint
| Cateutations
| Source: ATCC 22662
1
rIneoccoumlmuemnpdreedpamraetdiioun:m fAolrg4aldastyosckbewfaorsestuebsctuilntiutrieatdioinn. OECD
Use in obtain
atnesti:nitSiatloccekllcudletnusrietywaosf
diluted 1.0 X
1a0n%dceslpliskpederinmtlo
test (1.0
solutions to X 10 actual
concentration).
PNrae,paErDaTtiAo)n:to DOeEioCnDizerdecwoamtemrenwdaesdscpoinkceedntwriatthionnust.rieTnhtestmoocskst (rienccelnutding chemical analysis for deionized water is in Raw Data Package.
0C.ha2r2acftielrtirasttiiocns:priAotr ttoesutsteimien,thtehetemset.dium had a pH of 7.89. Sterilized via
Type: foam
Sterilized stoppers.
250-ml
borosilicate-glass
Erlenmeyer
flasks
sealed
with
rUespeliicantteessta:nd1t0h0remelroefplailcgaatlesmpeedrituemst osrubtsetstanscoelultoiaodni/ncghawmebreer.empSilxoyceodn.trol
During the test, were made.
kept
stoppered,
except
when
experimental
observations
Duration: 4 days.
Daily photoperiod: fluorescent lamps.
Continuous at Test chambers
740 held
ft-candles on shaker
using table
cool-white set at 100 rpm.
Temperature: 25.0 + 2C.
IBimoplroogivceadl:NeDuabialuyercelhlemcaocuynttsomuestienrg.direct microscopic enumeration via
aWnadtetremcpheermaitsutrrey,: ((2)1)daAitlyt-estteimnpietriaattiuorne.andAltlerdmetienramtiinoantipoHn,s cwoenrdeucmtiavdiety, |
according to APHA methods (1995).
|
I+, 2-, 3-, and 4-d ELgy's, and 4-d NOEL based on both cell counts and |
average specific growth rates (x).
|
SSppoonnssoorr: St3uMdy CIoOmNpPa2n0y25
4
C4273
EAnsveilroCnomrepnosrlstToens/tAiSncgl-DiDvuilsuitosn Ase Study 104 5010.06
4.0 RESULTS
ET Test
Substance
ToT xicity (Pertinen-- tdataare -- Cell Count 1-d-- ELgy: 15.8 mg/-- L (13.2-18.8)
]
in Tables 1, 2, and 3)
wd Egg: 15.9 mg/L (14.4-17.6).
I
|
Cell Count 2-d ELyy: <5.2 mg/L.
# 2d ELgy: >40 mg/L.
|
1Ce3lldCEouLnt;3-d>4EL0gym:g/L<.5.2 mg/L.
|
|
|
|
{
| |
I
Test Conditions
pCkel4ldCoEuLnt:4-d>4EL0gym:g/L2.0.8 mg/L (16.4-26.4).
Cell Count 4-d NOEL: 1.9 mg/L (1.62.5).
1 4-d NOEL: 18.9 mg/L (14.6-22.1).
1NCOyqECas vtahleueesndaproeinatp.proximations using the Ealfgfieccitdaolfbtaesstedsuobnsitahnecerewsualstsdoeftearmpionste-dtetsot be d10et2er4m0i0namtigo/nLwlhoeardeincgelfasilperdevtioouisnlcyreeasxepoisned nreusmulbtesrweafrteern9otdaaypsp.lieTdhteosededteefrimniitnievethteest EreLsguyl'tsedasintghreeaftinearltchealln c5o0u%ntsinfhoibritailolnl.oadings Temperature (C): 25.6-26.0.
I
L
_
Light intensityfi-cdls: 730-750.
|
PH: 784-1026,
|
Specific conductivity (umhos/cm): 131-160.
SSppoonnssoorr: Su3dMy CIoDmFpPa3n0y25
5
94276
EAnSvCilcoCnomrpeonraaTieosni/nAgSeDli-vDisuiloun "ASel Swdy 1DY 010.068
5.0 TEST QA/QC
os co oe] lAelagsatlacofuacnttosr ionfc1o6ntdruorlinmgusttheinfcirrsetas3e-dbyofatthe | cDouurnitnsgitnhcerefaisrsetd3b-yd aoffatchteortesotfcaopnptrroolxiamlagtaelly ||
exposure.
388.
I
Testduration mustbe 4d.
| Thetest durationwas 4-d.
7]
6.0 REFERENCES
=
rr----------------------
American Public Health Association (APHA). Water and Wastewater. APHA, Washington,
1995. D.C.
Standard
Methods
for
the
Examination
of
| OTersgtainnigzaotfioCnhefmoircEalcso.noOmEicCDCooPpueblriactaitoinonanIdnfDoervmaetlioopnmeCnentte(rO,ECWaDs)h.in1g9t9o3n.,ODE.CC.D Guidelines for
| GDiertleirnmg,inAi.nEg.,theG.AFq.uaWthiaclTeo,xiacnidtyDo.fM.PoMo.rlAydWeatme,r-S1o9l9u4b.leACGoumidpelleixneMiSxutpuprleesmeunstinfgorWaterAccommodated Fractions. Chemosphere, Vol. 29, No. 12, pp. 2645-2649.
Hamilton, M.A., R.C. for Estimating Median
Russo, Lethal
and R.V. Thurston, 1977. Concentrations in Toxicity
Trimmed Bioassays.
Spearman-Karber Method Environ. Sci. Technol.
I
117): 714-719; Correction 12 (4): 417 (1978).
US EPA. 1993. A Concentration (Cp)
Linear Interpolation Method for Sublethal Toxicity: The Inhibition Approach (Version 2.0). Environmental Research Laboratory, Duluth,
Minnesota.
SSppoonnssoorr. St3uMdyC1o0m4pPa3n0y25
5
0477?
TABLE 1. TEeLsgt,'wSi,th95(%3:C5onfidence Intervals, and 4-d NOEL'S from Algal Growth Inhibition
|| --com TF [ e~m e 1dEE E, DL, T m || o--e [ aoeae2ee18s8 ]||
|F oee m CE T Teoses]
--pepsi we
ee--
w14e.41s76 |
SES my Te cu
[venTeTne]
C0473
TABLE 2. cCealplrsicpoerrnumtlumanEdxpPoesrceednttoage#3D0if5ference from Mean Control Value forSelenastrum
WO | we [TT TT [cio
I
= TA
Lew
6
1
52
396
649 --
I
B
3
23
240
sn
`Mean
2.3 (48)
24.3 (-53)
243 (37)
572-17)
Mean
3.0 (33)
163 (68)
99 (:74)
422(:39)
Il
B
2
u
52
300
* 0-d Mean Algal Count = 1.0 X 10%
94279
TABLE 3.
[ RWBT| = we [= TT= T] T rr
Average Specific Growth Rates () and Percentage Difference Value for Selenastrum capricornutum Exposed to ps
from
MeanControl
ay A LT -- 1
| i
= oe oe[om[oo] REE EEE EET emLebS tr
pr ne
4280