Document jqJ8QV2yQZNpwNXbXda0N5qN

BIOSEARCH INCORPORATED P O- B O * B5* 8 ' PHILADELPHIA. PENNSYLVANIA 19IOI TELEPHONE: (219) 7 3 0-4 499 AR226-2907 P r o je c t Number; SUMMARY OF RESULT? OF AN ACUTE ORAL TOXICITY L D ^ STUDY Conducted f a n D ate o f R eport; E. I . du P o n t de Nemours Company H askell Laboratory JU ly 26, 1982 S -1 4 ,5 3 3 A c u te O ra l T o x i c i t y LD5Q - Male jRats. B ie LDS0 an d 19/20 C o n fid en ce L im its - 2 ,1 5 0 (1 ,9 6 1 - 2 ,3 5 8 ) mg/kg. i I Company Sanitized. Does not contain TSC CBI BIOSjEARCH INCORPORATED p.o. box esaja. Philadelphia. Pennsylvania ibioi TELEPHONE: 1219) 739-4490 Page i of 3 Subm itted to M aterial: Sample R eceived: Study I n itia te d : D ate o f R eport: T est: O bject o f T est: P ro ce d u re: jB. I . diji P o n t d e Nemours s Company H askell Laboratory E lkton Road ,, Newark, jDelawa 19711 E . I . d u P o n t d e Nemours fi Company H -14,533 4/30/82 T ~ 5/18/82 Study Completed: 6 /2 9 /8 2 7/26/82 A cute O r a l T o x i c i t y LDg0 - R a ts Vo s tu d y th e a c u t e o r a l t o x i c i t y i n r a t s o f t h e s u b j e c t m ateriale j - . Five groups o f te n bc' s alb in o r a ts o f th e eu fb red Sprague-- Dawley S t r a i n w e ig h in g b etw e en 200 and 300 gm e a c h w ere employed in t h i s s tu d y . The r a t s were d e p riv e d o f fo sd b u t n o t w ater o v ern ig h t p r io r to d o sin g . Each anim al was weighed and dosed by d ire c t ad m in istratio n o f th e experim ental m a te ria l in to th e stomach by means o f a sy rin g e and d o sin g n eed le.! The anim als ware housed and m aintained in com pliance w ith th e A nim al W e lfa re A c t (P u b . L -9 4 -2 7 9 ) 9 CPR P a r t 3 . Husbandry C o n d itio n s: T em p eratu re - 72*F _+ 2P R e l a t i v e H um idity - 45* jh 5* lig h t - j i 2 h o u rlig h t/d a j:k cycle D ie t - Wayne L a b -B lo x and t a p w a te r w are p r o v id e d ad lib itu m Based on our c u rre n t knowledge no contam inants were known t o be i n t h i s d i e t o r w a te r t h a t m ig h t be expected to in te rfe re w ith the o b jectiv es o f the stu d y . Caging 4 S ta in le s s s t e a l w ith e le v a te d w ire mesh flo o r in g j male ra ts /c a g a Bedding;- Techboard ; Shepherd P ro d u c ts Company ; Kalamazoo, M ichigan 49005 i Hie sam ple was dosed a s a 10% w /v s u sp e n sio n i n w a te rs i Company Sanitised. Does no! contain TSCACF4 Project Number: ?age B. I . du P o n t de Nemours fi Company - H-14 ,533 Acute O ral T o x icity LD^ S tu iy . Procedure (continued) * e follow ing dosage le v e ls were adm inistered , 1,580 mg/kg ' 2,000 mg/kg : 2,510 mgAg 3,160 mg/kg : 3 ,9 6 0 mg/kg F o llo w in g a d m i n i s tr a t i o n th e a n im a ls w ere - *____ , __, w ater ad lib itu m h r ,a 8 re allo w *d nd z s jg r * - s -s a r*- AhimajLa were o b se rv e d f r e q u e n t l y on th * . tw ice p er day th e re a fte r , d*y o <*osage, and S L S S L * ** ? . * . u ! , a R esults Conclusion o f th^ 14 d a , o b U r o .tto n fJ .r iId ? " *U U ^ ? 5Sl.,^ S S 1t o l2 . 2 P l? " , ? ? Thompson h o v ln , A ,, r . ,, a. 0 3 , " ! * . ' i a " 6 te i" > Soptosbor, 1952, v l l P^ra c e a ' P h ila d e ^ ,1U1ae, i " ** c h i , P e n n sy lv a n ia Of G iosearch, See T able 1 . i S " * 2 ,U*d in " 1* *" *> r o t . t a . f r o . i f , 6, t o SS , f 8 ^ A , r " " ' , / M C w ,fld " < U n i t . o f Th* V i eP r t i a i n com pliance w itn - *M ty Assurance O fficer A A ? / r i A .y^tiL B a rb a ra A. C a s t e l l , M.Ag. Laboratory Supervisor D ate 0?s$L&*uP K arl L . G a b rie l, V.M.D., P h .o .-- Study D ire cto r . Company Sanitized. Does not contain TSC C3J IOSCARCH. INCO RPORATED P O aox s a . p h il a o x l p h ia . p n n * y l v a n ia ib io i (213) 733*4493 % P r o je c t NumberI TABLE 1 a<* A cu te O ra l T o x i c i t y lD5q - Malee o? M a te r ia l: E. I du P o n t de Nemours a Company - h- U , 533IP C o n c e n tra tio n : 100 mg/ml O9OZ Number o f Animale J8 10% w/v Buspenaion in water. lO I l V I N V A - IA S N \ y t M r i ia a v - i iM r i . es* Los O r i O bservations : ,580 ^ g r W,e -- $ 0 and ^ fW e n e L im ite - 2,150 (1,961 - 2,358) m gA g. TT* **"1j ^ j a l w ^ r p r ^ ^ ^ ^ ^ S i c S ^ *t" 24 ho"* * of > ,0 0 0 mgAg - ^ S . n ^ i . o n aly . 4 . M 2 . 5.0 A i - S n S o t^ ** i "T ? *? *> e L d J < . . tt a e e t h . J * 8' r *0. 0.00 W . s u b s e q u e n tly o c c u r r e d ^ , o v S l d r L 3l "n t M , '" L aH " ? t M " P" * 1 * , t e t M h `>" r " - D e n ti 3. .o . , A , . v s r r - . , ** *w *, t e ', t o a " *y [ " " i n i " 3 " " ^ . " . p ! i n r " d,, t " , t t 2 4 ' h o u r ' o ^ t h e ' T w lth in " 20 b o u t . T I 3,980 - a , . A f f S L r s j; o v ,t " " " 1 w " - rs is r*-- .*- io0-- 1 ^ " is's ^ n rs ^ rir^ M rs r " * " " *"1**1' *" '* ` *` 9 I O D eervetlon p erio d l o , t e e l * . IT I P" h010910 -- -- a , renarkohle. he . Moving v e rm M e a , en n o d ln e a by M 1 , I U a . t t l n , Company Sanitized. Does Rol contain TSCACBI Page 3 o 3