Document jmdx7L1Ex25z0OG9v7yeONO8p

E. I. du Font dc Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT NO. 323-70 Material Tested: H, tc-rial Submitted by: 'Organic Chemicals Department, Jackson Laboratory Haskell No.: 6338 Other Codes ACUTE INHALATION TOXICITY Procedure: For each exposure, six ChR-CD male rats, weighing 250 to 280 grains, were exposed to the tes 16-liter bell jar for four hours unless all died sooner. In order to generate a uniform aerosol, the te of 1020 steel was heated to 130C(maximum temperature used in storage)and sprayed via compressed air in through a stainless steel pneumatic spray nozzle with an outside temperature of 150C. Chamber atmosph test material were determined periodically by filtering a known volume of the atmosphere through a filt pore size and determining the weigh*- increase of the paper and by a gas chromatographic method. The p tion me.- 'jrements were made with a cascade impactor. Gross and histopatholusic' examinations were perf dying di.L'ing an exposure and two rats surviving 14 days pose-exposure. The other sur.'ivors were sacrifi Results: Exp. .N2_ Anal. Cone. (mg/L) 1.8 4.1 Particle Mass Median Diameter (p) and _Dispersion (g)J= 3.8 & 2.4 Mortality Ratio 0/6 1/6 6/6 Clinical Signs During Exposure Irregular respiration, red ears Irregular respiration, paleness; one out of six rats had convulsions, death after second hour Irregular respiration, pale ears;, one rat had convulsions, deaths occurred between 2^, and 31-- hours of exposure Initial by nor 10-147 one rat body w normal The generated aerosol formed a paste-like material on the walls of the bell jar and made it difficult ffesapsssySanitiz AR226-2827 - 2 - Pathology: and edema. Two rats that died during exposure to greater than 7 mg/L of the test material, had acute pul Two rats, sacrificed 14 days post-exposure to 1.8 mg/L, had lymphoid hyperplasia of the sple tracheltis; one rat also had a focal area of hemorrhage in one lobe of lung. Sunnnary: The Approximate Lethal Concentration (ALC) ot| Histopathologic results showed acute pulmonary edema anahemor rhage. jis 4.1 mg/L for a four-hour in therefore less toxic than either Haskell Nos. 5904 or 6047 under the test condition + Tissues examined include: lung, duodenum, and bone marrow. ^ Geometric Standard Deviation. trachea, kidney, liver, spleen, brain, adrenal, testes, stomach, hea Experimental Work by: Report by: (^"n^^i_ ' 0Fige Assist Inhalatio 2Xfr>^tA DD<AftTo Inhalatio POTrDAS/pgh -- Uate: July 31., 1970 Approved by: A^.. John Company Sanitized.