Document jmOpzK6mQe962ajzgdqoq2Zx9

A ft #.9.6 _ > 73/ ACUTE TOXICITY TO AQUATIC INVERTEBRATES (ACARTIA TONSA) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203. Current information indicates it is a mixture of 1.34% PFOS, 35% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: ISO/DIS 14669-1997: Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea). Type: Acute static GLP: No Year completed: 2000 Species: Acartia tonsa Supplier: Test organisms cultivated from eggs, supplied by Marine Biological Laboratory, University of Copenhagen, Denmark. Analytical monitoring: Dissolved oxygen and pH Exposure period: 48-hours Test organism age: Large copepodites and adults Statistical method: LC5ovalues calculated by PROBIT analysis, National Swedish Environmental Protection Board (Version 2.3,1990). Calculation of NOEC by single factor ANOVA followed by Bonferronis procedure. Test conditions: Dilution water: Filtered natural seawater (34 ppt salinity) from an unpolluted site in Byfjord, Norway. Dilution water chemistry: Not given. Lighting: A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A 1003 mg/L primary stock solution was prepared by diluting 0.5017 grams of test sample in 1 liter of water. Aliquots were then added directly to water in test vessels to create test solutions. 000718 Remarks: There appears to be an error in the stock solution preparation information. Dilution of 0.5017 g of test substance in 1 L of water will not give a stock solution of 1003 mg/L. Based on review of another report conducted by the same laboratory in the same period, the stock solution preparation information should be dilution of 0.5017 g of test substance in 0.5 ml_ of water to give a stock solution of 1003 mg/L. Exposure vessels: 28 mL rolled rim vials containing 25 mL of test solution Number of replicates: 4 Number of organisms per replicate: 5 Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-48 hours): 8.1 -8 .1 (control exposure) 8.1 - 8.2 (320 mg/L exposure)* Temperature range: 20 1 C Dissolved oxygen range (0-48 hours): 7.2 - 7.6 mg/L (control exposure) 6.1 - 8.0 mg/L (320 mg/L exposure)* * 320 mg/L (second highest concentration) exposure values used because the highest concentration resulted in total mortality by 24 hours. RESULTS___________________________________________________ Nominal concentrations: Bk control, 10, 32, 100, 320, and 1003 mg/L. Element values: 24-hour LC50 = 340 (260 - 460) mg/L 48-hour LC5o = 200 (140 - 270) mg/L 48-hour NOEC = 100 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The FC-203 48-hour LC50 was determined to be 200 mg/L with a 95% confidence interval of 140 - 270 mg/L. The 48-hour No Observed Effect Concentration (NOEC) was 100 mg/L Validity criteria fulfilled and reference substance in normal range. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 000719 DATA QUALITY Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-2, 2000. OTHER______________________________________________________ Last changed: 6/27/00 000720 99312-2 8l l NORSK AKKREDTERING ROGALAND RESEARCH "FC-203 Light Water Brand AFFF" Toxicity tests Report RF-2000/002 Lab. ref. no.: 99312-2 No. of pages: 31 ISBN: Author: Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 / 06. 01 2000 Research Program: Open from (date): Summary: The following test results were obtained with "FC-203 Light Water Brand AFFF": Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h EC* 48 h LC* 10 d LC* (mg/l) : 62 (mg/l) 200 (mg/kg dry weight) : >3060 The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this document may be reproduced in any form without permission in writing by RF - Rogaland Research . (S tudy re c to r y Inger Lisa Andersen for RF - Rogaland Research Kre Netland 000721 L99at3i1.r2e-j2.no.: r . i/; CONTENTS: 1. Preface.............................................................................................................................................................3 2. Test for toxicity with Skeletonema costatum.............................. 4 3. Test for toxicity with A cartia tonsa..............................'.............................................................10 4. Test for toxicity with Corophium volutator.............................................................................15 5. Data sheet(s) ...............................................................................................................................................20 000722 99312-2 ' "O PREFACE: Ecotoxicological tests were Novatech. The sample is a cpoenrfcoernmtreadt,eotnha"FtCn-o2r0m3aLlilgyhwt Willabteer BusraenddinAFaF3F%"insoalugtrieoenm. eTnet swtsithwere conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995 / SFT1998). Theproject was carried out at RF - Rogaland Researchfor 3M Norge. All the tests were performed by RF - Miljelab. Study director was Senior Engineer Inger Lisa Andersen. The test results arepresented in separate sections. The results relate to theproduct as supplied by Novatech. TThhiesatcecstrerdeiptoartitoins isstasuteesdtbhyatathlaebloarbaotroartyoaryccmreedeitstetdhbeyNNAorrewqeugiriaenmAenctcsrceodnitcaetironnin(gNcAo)m. petence, equipment and workingproceduresfor all the test methods contained in the accreditation. Iint taelrsnoasltqauteaslitthyactotnhterolalbsocrhaetmoerys,hpaasraticsiaptaistfiaocntoinryinqtuearllaitbyoarsastuorraynccoemspyastreismo,nws,haicnhditnhceluudseesof certified reference materials and secondary reference materials. The testsperformed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without thepermission in writing by RF - Miljolab. 000723 T est for T oxicity Marine alga: Skeletonema costatum 000724 99312-2L.UU.I L J . h li.. TEST REPORT GalRgOaWTSHkIeNlHeItBoInTIeOmN TaEcSTosWtIaTtHuTmHE MARINE (St NORSK AKKRED1TERING Nr. POH ROGALAND RESEARCH TEST SUBSTANCE: "FC-203 Light Water Brand AFFF" TEST RESULT: I The 72 hours EC50was calculated to 62 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: NwSit-hESNkeIlSeOton1e0m25a3c,o1s9ta9t8u:mWaantderPqhuaaeloitdya-cMtylaurminetraiclgoaml ugtruomw.th inhibition test TEST ORGANISM: Skeletonema costatum, organism is kept at RF strain NTVA BAC 1. - Rogaland Research. A stock culture o f the test PREPARATIONS: Naasnadtbuabrsraiislesffeolyarwhtheaaetetgerrd(ostwaoltiahnbimtoyue:td37iu45m%C.o)pTfrrhiooemrwtoaatnueursenw.paoTslhlfueilttesederaeswditaettheirnrowBugyahfsjoaernGdriFwc/hCaesdfuiwlsteeitrdh, nutrients according to ISO 10253, and recommendations from the Paris Commission. Tmmeges/d1tisuwomalus. tpiOornethpseawrreecdroenbcpyernsettpirraarrtieinodgnf0sr.ow5m0e0rae8spgtrorecapkmasrsoeodluffttrihooenm.prtAhodissutscottcoikcnks1osloluiltutriteoiononfo.gfr5o0w0t.h8 INCUBATION: Tolaretbseitdtactluoslthaubarkoeuesrto,2f305r00e0pmclielclawlstee/mrsefloifnrrcoeumabcahatnecdoenxincpeo1nn0ter0namttiiaolnlfl.ylaTtg-rebosowtttcionumgltueindreofsclauwsluekrmseoicnnuoaltcnuure. The test was run under continuous light (> 50 pE/irr-sek) from fluorescent^ " tubes of universal natural white type, at a temperature of 20 2C. The test was run for about 72 hours, and subsamples of 5 ml were taken from tbhye ftleusot rfelascskens ceea. chpHdamy efaosrugrreomwetnhtsmweaesruerpeemrfeonrtms. eAdlgasalingdriocwattehdwoans dmaetaassuhreeedt. 000725 L99a3b1.r2e-f2.no.: Page 6 of 31 DATA ANALYSIS: CALCULATIONS: Growth rates were calculated by log linear regression on biomass (fluorescence) versus time, as described inNS-EN ISO 10253. ErCso: Tceofhfneeccietnnchtoribnaitctieoonrnytroeafftfiaoepnctraoondndutcthhteeggi9vr5io%nwgtch5o0on%ffiSdrkeeendlcueetcotliniomenmitoawfcgeorrsoetwacttauhlmcrualitasetee(xEdprbCryessots)he.edTuahsseisthoef the computer programme TOXEDO developed by VKI (Denmark), assuming a logarithmic normal distribution of data. The programme also calculate concen trations inhibiting growth rate 10% and 90% (ErCio and ErCgo). NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: COMMENTS: VALIDATION: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance E,Cio ErC5o ErCso "BFraCn-d20A3FLFigPht Water NOEC: 32 13 (8.3 - 18) 62 (48 - 83) >100 iVVnaatelluurveessalaisnradericceaaitlnecducilocaantetcedednb.tyrNatthOieoEncCsogmairpveuintcegarl1pc0ur%olag.tre5ad0m%bmyaeunsTdinO9gX0tE%hDeiOcnoh(miVbpKituiIot,enDropenrfogmgraorrawkm)th,marenatdGe.9r5aU%phnPictosandinfiIdnmeSgnt/acl.et 3.0. The results are based on growth rates for the time span 0 - 72 h, the period during which the growth is considered to be exponential in the control cultures (see growth curves). The validity criteria given in the test protocol are fulfilled, in that the control cell density increased by a factor of more than 16 in the test period (corresponding to a growth rate of more than 0,9 d'1), the control pH did not _-- v3a,5r-ydbicyhmloororephthenanol1w,0aus ninitsthdeurrainnggethneotremstaallnydfothuend7.2 hours test results with Lab. ref. no.: Sample received.: Test period: Report date: 99312-2 20.09.99 04-07.10.99 06.01.2000 EElinngHinoerevre Analytical technician SenQiourgaRelieMtsyeoAalvrsceshrusrSmacnyicerentist Enclosed: Datasheets Tfohrims toesthterrepthoarnt iisnisfsuulle,dwbityhoaulat bthoeraptoerrymaiscsciroendiitnedwbriytinNgobrwyeRgFia-nMAiclcjoreladbi.tation (NA). The report may not be reproduced in any 00072b PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: 99312-2 Start date: 04.10.99 Cone, unit: mg/l Measurement: The biomass was measured by fluorescence; values in fluorescence units Time: 04.10.99 05.10.99 06.10.99 07.10.99 Concentration 14:05 09:25 10:30 10:40 Growth pH pH rate Corre- at start at end day -1 lation of test of test 0 0.11 0.59 9.2 56 2.39 0.998 0 0.15 0.63 7.9 59 2.14 0.997 0 0.13 0.62 9 54 2.29 0.997 8.3 8.4 0 0.14 0.63 6.4 47 2.07 0.999 0 0.14 0.62 6.3 48 2.06 0.999 0 0.12 0.71 7.1 55 _ 2.20 1.000 0.32 0.32 0.32 0.13 0.82 9.2 0.13 0.77 9.6 0.14 0.84 12.5 61 66 71 2.30 2.32 2.43 1.000 1.000 0.999 8.4 8.4 1 0.14 0.77 11 70 2.36 0.999 1 0.14 0.69 12 65 2.41 0.996 8.4 8.5 1 0.16 0.63 8.3 60 2.13 0.995 3.2 0.18 0.58 8 63 2.05 0.990 3.2 0.14 0.59 7.1 52 2.12 0.997 8.4 8.5 3.2 0.12 0.6 6.6 50 2.17 0.999 10 0.14 0.58 8.5 64 2.22 0.995 10 0.16 0.34 4.4 48 1.79 0.974 8.4 8.5 10 0.16 0.6 7.6 68 2.09 0.994 32 0.14 0.43 4.4 42 1.86 0.992 32 0.12 0.39 5.3 46 2.05 0.990 8.4 8.4 32 0.13 0.3 3.6 30 1.79 0.979 100 0.1 0.04 0.1 0.3 0.00 100 0.09 0.18 0.3 1.35 0.65 0.987 8.4 8.2 100 0.08 0.06 0.21 1.3 0.52 0.784 Reference substance (3,5-dichlorophenol): 1.5 mg/l 0.14 0.47 1.85 1.5 mg/l 0.16 0.43 1.45 1.5 mg/I 0.16 0.45 1.45 Mean values: 0 0.32 1 3.2 10 32 100 0.13 0.13 0.15 0.15 0.15 0.13 0.09 Reference subst. 0.15 0.6 0.8 0.7 0.6 0.5 0.4 0.1 0.45 8 10 10 7 7 4 0 1.6 4.2 3.3 2.9 53 66 65 55 60 39 1 3.5 1 .3 9 1 .1 9 1 .1 9 0 .9 9 9 1 .0 0 0 0 .9 9 9 8 .3 8 .2 * 2 .1 9 2 .3 5 2 .3 0 2.11 2 .0 3 1 .9 0 0 .3 9 1 .2 6 0 .9 9 8 1 .0 0 0 0 .9 9 7 0 .9 9 6 0.991 0 .9 8 8 0 .9 1 7 1 .0 0 0 The result of the reference substance is within the range normally 1foun ) 0 0 7 2 l9a9b3.1r2ef-.2no.: Page 8 o f31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: 99312-2 000728 inhibition of the growth of Skeletonema costatum with: mg/1 (FC-203 Light Water Brand AFFF) Experimental data: Concentration in Growth rate FC-203 Light Water Brand AFFF Inhibition in percent Control 1 Control 2 Control 3 Control 4 Control 5 Control 6 Control mean : 0.32 0.32 0.32 1.00 1.00 1.00 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 2.39 2.14 2.29 2.07 2.06 2.20 2.19 2.30 2.32 2.43 2.36 2.41 2.13 2.05 2.12 2.17 2.22 1.79 2.09 1.86 2.05 1.79 0.00 0.65 0.52 - 0 0.0 0.0 0.0 0.0 0.0 2.8 6.5 3.3 1.0 0.0 18.3 4.6 15.1 6.5 18.3 100.0 70.3 76.3 STATISTICAL PARAMETERS CALCULATED FROM CONTINUOUS RESPONSES 5 doses and 11 responses have been used during calculations. EC 10 : EC 50 : EC 90 : 13.33 61.95 288.00 95 % confidence limits : ( 95 % confidence limits : ( 95 % confidence limits : ( 8.29 .. 48.25 .. 186.95 .. Coefficient of determination :0.763 18.46) 83.10) 562.40) 000729 L9a9b31.r2e-f2.no.: Page 10 of 31 T est for T oxicity Marine herbivore: Acartia tonsa 0007G0 9931.. 2*.-j2 . <" d- ' ' v - ' TEST REPORT AcartiaACUTE TOXICITY TEST WITH marine copepod TtoHnE sa NORSK AKKREDITERING Nr.POIf TEST SUBSTANCE: "FC-203 Light Water Brand AFFF" TEST RESULT: The 48 hours LC50was calculated to 200 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ItoSOm/aDrIinSe1c4o6p6e9po- d1s99(C7:opWepatoedraq,uCarliutsyta-cDeae)t.ermination of acute lethal toxicity TEST O R G A N ISM : Acartia tonsa University of (Dana), supplied Copenhagen. by the Marine Biological Laboratory, CULTIVATION: Twanhitdehattedhsuetlot"srPgwaarenirsiesCmuossmewdmeirinsestihsouenptpe-slRite.idngasTeegsgt sP,raontodcwole"re(VcKulIt)i.vaLteadrgiencaocpcoeprdoadnitcees PREPARATIONS: Npasraitbourarstaiolsusfeosarew.thaetedri(lusatiloinnitwy:at3e4r.%To)hferowmataernwunaps ofillltuetreeddstihteroinugBhyafjGorFd/Cwafisltuersed Tmweagst/1etrsw.oalOusttpihorenerpscaworneedcreebnpytrresatptiiarorrniensdgwf0reo.r5me0p1ar7esgtporacarkmedssoforluoftmtihoenth.pirsAosdtsuotccoktciksnosl1oulltuiitotrineo.nofodfi1lu0t0i3on INCUBATION: The test was run in glass containers (28 ml rolled rim vials) each containing 25 ml of test solution and 5 animals, 4 replicates for each concentration. The vials were kept under a 16h/8h light/dark photoperiod at a temperature of 20 1 C. pH and oxygen measurements were performed as indicated on the data sheet. 000731 L99a3b1.r2e-j2.no.: tage u uj j i DATA ANALYSIS: The effect on the survival ofAcartia tonsa is expressed as the concentration of paeprrtoedsutcctolnecthenatlrtaoti5o0n%weorfethuesetdesftoarnciamlcaulsla(tLioCn5.0)T. hTeh2e4cohmoubrisnaenddm4o8rthaoluitrys LstCan5d0avradlupersocaenddutrheeP9R5%OBcIoTnfAidneanlycseisli,mNiattwioenraelcSawlceudlaisthedEbnyviurosinnmgetnhteal Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CcfaoacnltccouernlAattrNiaotOnioVonfAmNfeOoalEnloCwwi(etNhdotbhOyebBcsooennrtfvreeordrlomEnfeifsaenpc.troCcoendcuernetfroarticoonm) ipsadroinngeebaychsingle- Test results: Table 1. LC and NOEC values obtained with "FC-203 Light Water Brand AFFF'. Acartia tonsa 24 hours 48 hours LC10 160 (84-220) 76 (32-110) LC50 340 (260-460) 200 (140-270) LC90 740 (530-1400) 510 (350-1100) NOEC: 100 100 ctVhaaellcucueolsamitpnedudtibecyraptPerRo1gO0r%Ba.ImT5m0ae%naGlayrnsaidps,9ha0P%naddl9Ien5thS%atalcittoy3n.a0fif.dteern2ce4 ianntedr4va8lhsoaurres.indUicnaittsedin. mNgOflE. CVaarlueecsaalcreulated by V alid a tio n : The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end of the test was greater than or equal to 4 mg/1, the percentage mortality in the controls was less than 10%, and the 48 hours LC50 of 3,5-dichlorophenol (reference) was in the range normally found. Lab. ref. no.: Sample received.: Test period: Report date: 06-08.10.99 06.01.2000 AnalyEtEliicnnaglHintoeercevhrenician SenQiouraRlietsyeAarscshurSacniceentist Enclosed: Data sheets e,dwbityhoaulat bthoerapteorrmy iascsciroendiitnedwrbiytinNgobrwy eRgFia-nMAiclcjareladbit.ation (NA). The report may not be reproduced in any 000732 99312-2 -'o PRIMARY DATA: Acute toxicity test with Acartia tonsa Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: Start date: Measurement: 99312-2 06.10.99 Number of dead Concentration (mg/I) Control 10 32 100 320 1003 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 5 55 30 5 5 5 5 55 20 5 5 55 20 5 5 55 20 5 5 55 20 5 20 ' 5 55 5 NUMBER OF DEAD 24 hours 48 hours ACE BDF Total ACE BDF Total 0 00 0 01 000 1 101 2 0 00 0 0 10 00 1 0 00 1 001 11 2 1 00 201 0 1 1 4- 2 12 344 2 7 3 14 5 55 5 55 5 20 5 20 Reference substance (3,5-dichlorophenol): 1.0 mg/l 5 55 0 20 2 54 30 5 55 100 3 130 The result with the reference substance is within the range normally found. 15 000733 993 J 2-2 Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: Start date: 99312-2 06.10.1999 Measurements of oxygen and pH. Concentration (mg/1) Start Oxygen (mg/l) pH 24 hours 48 hours Oxygen pH Oxygen (mg/l) (mg/l) Control 7.6 8.1 7.2 10 8.1 8.1 7.3 32 8.1 8.1 7.1 100 8.1 8.1 6.7 320 8.0 8.2 6.1 1003 7.3 8.2 6.6 8.1 pH 8.1 8.2 8.2 8.2 8.1 Reference substance (3,5-dichlorophenol): 1.0 mg/l 7.7 8.1 7.3 8.2 000734 T est for toxicity Marine Amphipode: Corophium volutator 000735 L9a9b3.1r2e-j.2no.: Page 16 oj'31 TEST REPORT ACUTE TOXICITY m arine amphipo SdEDCIMorEoNpThTEiuSTmWvIToHluTHtaEtor (Si NORSK AKKREDITERING Nr. POI ROGALAND RESEARCH TEST SUBSTANCE: "FC-203 Light Water Brand AFFF" TEST RESULT: - The lOd LC50was calculated to >3060 mg kg'1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ATSoxTiMcityETl3e6st7s-9w0i,thStManadrainred aGnudidEestFuoarriCneonSdeudcimtinegnt1s.0 day Static Sediment TEST ORGANISM: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in aerated seawater feeding. at 15C. A small amount of natural seaweed was used for Preparation: NwaSntaaaatsvulyaursnaseelgddeserffaoowwrraaopstrreugeraspe(nasdiracilfnicongorinttdyeties:ltnu3stt4eiaod%nnimdow)peafnrrtoteosprm..orTaStniheodeuninsmsepidleotnilmfltuocertneoedltaleiscscihttaeetdeminsatu.Btdaydnfyjousrandnpedonlalounutdetdswidsaieste Each test concentration was prepared by adding the product in 600 grams of bduamckpetsewdaims sehnatkiennafporla3sthicoubrusc.keTth. e2s0e0dimmleonft seawater was added and the was divided into three beakers, 400 ml of seawater was added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample of tchaelcdualamtepdsaeds immgenktgw'1assedainmaelynsteddifyowr weiagthetr. content, and all concentrations are INCUBATION: The test was run in glass containers (600 ml) each containing 200 g of test sceodnicmenetnrta,taiopnp.. 4T0h0embelaokfesresawwearteerkaenpdt u1n0daenricmonaltsin, u3oruesplaiecraatetisonfoarteaach tienmdipceartaedtuorenothfe15data1shCe.etp. H and oxygen measurements were performed as 000736 99312-2 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Corophium volutator is expressed as the concen tmvraaoltruitoeanlaiontydf patehprerto9ed5su%t cctocnloecntehfniadtlretanotci5oe0nl%iwmeoirtfeatruheesectdaeslfctouarlnacitameldcaulbslya(tLuiosCnin5.0g)T.thhTeehs1et0acndodamayrbdLinCe5d0 PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CfaacltcourlAatNioOn VofANfOolEloCw(eNdobOy bBsoenrfveerdroEnfifsepctroCcoendcuernetrfoarticoonm) ipsadroinngeebaychsingle concentration mean with the control mean. TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-203 Light Water Brand AFFF" Corophium volutator 1 0 days LCio >3060 LCso >3060 LC90 >3060 NOEC: 3060 - GVVaarllauupeehssPainarddeiIccnaaStlectau1tl0a3%t.e0,d. 5b0y%PRanOdB9I0T%anlaeltyhsaisli.tyNaOfteErC1a0rdeacyas.lcuUlantitesdinbymtghekcgo'1msepduitmerepnrtodgrryawmemigeht. VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved otFhxleuyopgreeanrnccteeonnnteacge(rneetmfreaortreiotnancleiat)ytwtihnaestheinnedcthooenftrtrahonelsgteewsnetorwremalseasglslryethafoateunrn1tdh5.a%nTahonredesqeadutiaemlstteownt6itu0hs%ed, is found to be within accepted limits for organic content and silt fraction. Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction(%) 0 .8 LTSRaaeembspt.opprrleteefrd.riaonetdcoe:e.::ived.: 03-219039..3011922..99-929 06.01.2000 Elin Horve ge Molversmyr AnalytEicnaglinteecehrnician Senior Research Scientist Quality Assurance Enclosed: Data sheets Tfohrims toesthterrepthoarnt iisnisfsuulle,dwbityhoaulat bthoeraptoerrmy iascsciorendiitnedwrbiytinNgobrwyeRgFia-nMAiclcjoreladbit.ation (NA). The report may not be reproduced in any 000737 99312-2 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: Start date: Measurement: 99312-2 03.12.99 Number of dead Total number Number of specimen pr. vessel NUMBER OF DEAD 5 days 10 days Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F ACE BDF Total ACE BDF Total Control 10 10 10 60 10 10 10 000 000 0 10 10 10 91.1 30 200 2 10 10 10 501 30 101 2 1030 10 10 10 30 100 1 3060 10 10 10 30 102 3 Reference substance (fluoranthene): 20.3 10 10 10 30 322 The result with the reference substance is within the range normally found. 7 000738 L99a3b1.r2e-f2.no.: Page 19 o f31 Test substance: "FC-203 Light Water Brand AFFF" Lab. ref.: Start date: 99312-2 03.12.99 Measurements of oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 91 8.1 91.1 96 8.1 93 8.1 501 77 7.8 92 8.1 1030 97 8.1 93 8.1 3060 97 8.1 74 7.7 10 days Oxygen (%) pH 91 8.0 88 8.1 90 8.1 86 7.9 91 8.0 Reference substance (fluoranthene): 20.3 95 8.1 90 8.1 90 8.1 000739 L9a9b3.1r2e-f2.no.: Page 20 o f 31 Data Sheets 000740 L9a9b3.1r2e-f2.no.: Page 21 of 31 HMS-DATA3LAD (Kelse-, Milja- og Sikkerhetsdatablad) saaassssasssassaaaaaassasasssaassaiaaassasasassssasasssBsssasaaasssvaaass Dokumentnr.: Erstattet : 10-3814-0 Utgitt : 03/11/98 Versjon _ : 06.00 03/10/97 Skrevet ut : 11/11/98 Side 1 av U KMS-databladet er utarbeidet av Miljeavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 83 1 IDENTIFIKASJON AV KJEMIKALIET OG ANSVARLIG FIRMA. Importer: 3M Norge A/S Handelsnavn: FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Deklaras jor.snummer, Produktregisteret: P-nr.: 002614. Telefon, Giftir.fomasjor.ssentralen: 22 59 13 00 2 OPPLYSNINGER OM KJZMISK SAMMENSETNING. Informasjon om enkeltkomponenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VEKT-PROSENT VANN 7732-18-5 2-(2-3UTOKSYETOKSY)-ETANOL / 112-34-5 (DIETYLSNGLYK0LM0N03UTYLSTER) Faresymboler/risikosetninger: Xi; R: 36 1,2-ETANDIOL 107-21-1 Faresymboler/risikosetninger: Xn; R: 22 SYNTETISKE DSTSRGENTSR FLUORALKYL OVERFLATEAKTIVE STOFFER Trade Secret Trade Secret RESTER AV ORGANISES FLUORXJEMIXALISR Trade Secret 36 - 40 = 35 = 20 1-5 1-5 Ikke kjent 3 VIKTIGSTE FAREMOMENTER. 000741 L9a93b.1r2e-f2.no.: Page 22 of 31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Viktigste skadevirkninger Heisefare: Kan irritere aynene og huden. Sikkerhet: Miljafare: Spesialavfall. 4 FSASTEHJELPSTILTAX. Tiltak ayne: Skyll straks aynene med rikelige mengder vann. Kontakt lege umiddelbart. Tiltak hud: Skyll huden med rikelige mengder vann. Evis irritasjonen vedvarer, kontakt lege. Tiltak inn&nding: Hvis symptomer oppstr, bring vedkommende bort fra ekspor.eringskilden. Sarg for frisk luft. Hvis symptomene vedvarer, kontakt lege. Tiltak svelging: Kontakt lege umiddelbart. Fremkall brekninger kun i samrd med medisinsk personell. Gi aldri noe gjennom munnen til en bevisstlas person. 5 TILTAK VED BRANNSLUKNING. Egnet brannslukningsmiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: Forbrenningsprodukter ved brann: Prosedyrer ved slukking av brann: o o o ?42 L9a9b3.1r2e-f2.no.: Page 23 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM 6 TILTAK VSD UTILSIKTST UTSLI??. Personlige vernetiltak: Se vernetiltak under andre punkter i dette HMS-databladet. Tiltak ved utilsiktet utslipp: Ventiler omridet. Spill avgrenses umiddelbart. Dekk til med et absorberende materiale- Spill sarnies opp umiddelbart. Rengjor omridet med vann. Plasseres i en egnet beholder med lokk. Fjerning av kjemikalieavfall: Se punkt 13, Fjerning av k jemikalieavf all.j- I 7 ENDTERING OG 0PP3EVARING. ! ! I 3etingelser for sikker oppbevaring: Mi ikke lagres i omrider hvor produktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur under 49-grader celsius. Lagres ved temperaturer over 0 grader celsius. Hold behoideren tett lukket r.ar produktet ikke er i bruk. Uforenelige materialer: Ventilasjon: Oppbevar emballasjen pi et godt ventilert sted. Sikkerhetstiitak for k forhindre brann: Sikkerhetstiltak for forhindre eksplosjon: Ikkebrennbar-. Sikkerhetstiltak for i forhindre statisk elektrisitet: Forholdsregler ved bruk: Hold emballasjen tett lukket. Royking forbudt: Under bruk av produktet kan reyking forirsake forurensning av tobakken og/eller rayken og farlige dekomponeringsprodukter kan dannes. Se punkt 10, stabilitet og reaktivitet, skadelige dekomponenter. 8 EKSPONERINGSKONTROLL OG PERSONLIG VERNSUTSTYR. 99312-2 Page 24 of 31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Oyevern: Unngi i fk damp, spray eller take i oynene. Bruk tette vernebriller med ventiier. Hindvern: * 3ruk egnece verr.ehar.sker av hensiktsmessig materiale ved hindtering av produktet. Vernehansker av folgende materiale(r) anbefales: Butylgummi. Annet hudvern enn hindvern: Unngi hudkontakt. Andedrettsvern: Unng inninding av lufrbiret materiale. 3asert pi konsentrasjonen av luftforurensninger i arbeidsatmosfaren v^lges en av folgende type(r) godkjent andedrettsvern: halvmaske med filter mot organiske damper og vaskeformige partikler: A/R2SL. Svelging: Det mi ikke spises, drikkes eller roykes under arbeid med produktet. Vask utsazte omrider grundig med sape og vann. Vask hendene etter hindtering og for spising. Anbefalt ventilasjon: Hvis utsugningsventilasjon ikke er tilstrekkelig, mi- egnet godkjent incedrettsvern benyttes. Sorg for tilstrekkelig ventilasjon slik at campkonser.trasjonene holdes under anbefalte administrative normer og/eller kontroller spray eller tike. Adm. normer for forurensning i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Anmerkninger: Ingen adm. norm. 2- (2-3UT0KSYET0K5Y)-ETANOL / (DISTYLSNGLYK0LM0N03UTYLSTSR) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikar.sk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). 1,2-ETANDIOL (107-21-1) 8-timers verdi ppm: - (stov) 8-timers verdi mg/m3: 10 (stov) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (dam?) SYNTETISKE DETERGENTER (Trade Secret) Anmerkninger: Ingen adm. norm. FLUORALKYL OVERFLATSAKTIVE STOFFER (Trade Secret) Anmerkninger: Ingen adm. norm. RESTER AV ORGANISKE FLUORKJEMIKALIER. (Trade Secret) 8-timers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. o o o 44 9~9a3o1.r2e-j2.no.: Page 25 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM 9 FYSISKE OG KJSMISKE EGENSKAPER. :.Tistandsform, farge, lukt: _* Klar, ravfarvet vaske. ca. 8.5 Kokepunkt/-intervall: ca. 100 C (Begynnende) Smeltepunkt/-intervall: Ikke relevant Flammepunkt : Ingen (Setaflash CC) Selvantennelighet: Ikke relevant Eksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, avre: Ikke relevant Damptrykk: ca. 14.3 mmHg (Kalkulert v/romtemperatur) Laselighet i vann: Blandbar. Spesifikk vekt: ca. 1.05 (vann*l) Damptetthet: ca. 1.21 (luft=l) (Kalkulert v/20C) Flyktige organiske forbindelser (VOC): = 578 g/1 SCAQMD Kalkulert v/20C. Fordampningshastighet : < 1 (butylacetat=l) Viskositet: Ikke kjent ! Ol-i 10 STABILITET OG RSAXTIVITET. Forhold som skal unngis: Materialer som skal unngis: Ikke kjent. Farlige spaltningsprodukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eller partikler. Dekompopering av bruksferdig blanding forventes ikke i representere noen risiko. 000745 L9a9b3.1r2e-f2.no.: Page 26 o f31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Stabilitet og reaktivitez: Stabili. Farlig polymerisesjon vii ikke forekomroe. 11 OPPLYSNINGER OM HELSEFARE. Effekter ayne: Mild eyeirritasjon: svmpiomer kan vare redhez, hevelse, smerter og tarer. Effekzer hud: Mild hudirfitasjon (etter langvarig eller gjentatt kontakt): symptomer kan vare radhet, hevelse og klae. Effekter innandir.g: En enkelt overeksponering som overstiger anbefalte administrative normer, kan fare til: Pavirkning av santralnervesystemet: symptomer kan vare hocepme, arhet, sevnighez, mangiende koordinasjonsevne, nedsatt reaksjonsevr.e, slarez tale, svimmelhet og i ekstreme tilfeller bevisstlashet. Irritasjon (evre luftveier): symptomer kan vare srhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsak utfcrz med organiske fluorkjemikalier, som finr.es i dette produktet, viser effekter som leverforszyrrelser, vekttap, mangiende appetiti, slavhet samt effekter som pvirker neurologi, bukspytt, binyre og hematologi. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhod til veiledning. Svelging kan- fere til: Pvirkning av r.yrene: symptomer kan vare redusert urinmengde, blod i urinen og rvggsmerter. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer kommer ned i lungene. En alminnelig rsak til tilstanden er oppkast.): symptomer kan vare hosting, pustevansker, gispmg, hoste opp blod og lungebetennelse som kan vare livstruende. Informasjon om allergi, overfalsomhet: Kreftfremkallende egenskaper: 000746 L99a3b1.r2e-f2.no.: Page 27 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Arvestoffskadeiige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Et resym1 vedrarende produktets giftighet er tilgjengelig p foresparsel. Dette produktet inne'nolder en eller fiere organiske fluorkjemikalier, som muligens kan absorberes og lagres i kroppen over lengre tid, enten som utgangsmolekylet eller som nedbrytningsstoffer (metabolitter), og kan akkumuleres ved gjentatte eksponeringer. Det foreligger ingen kjente helseeffekter for mennesker fra eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Forekomsten av organiske fluorkjemikalier i blodet til den generelle befolkningen og underpopulasjoner som arbeidere, har vsrt publisert sicen 1970 arene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skacelige effekter. 12 OPPLYSNINGER OM MILJ0FARS. Persistens/Biologisk necbrytbarhet: Dette produktet inneholder en eller flere organiske fluorkjemikalier son muligens ikke er nedbrytbare og forblir i miljaet {persistente). Andre skadevirkninger, milja: Nedbrytbarhet: COC (Chemical Oxygen Demand) = 1 , 1 g/g; 30D 20 dagers (Biochemical Oxygen Demand) = 1 , 1 g/g; BOD 20 dagers/COD = 1,00. Akvatisk giftighet: Statisk 96 timers LC50 for fisk (Fathead minnow (Pimephales promelas)) = 750 mg/1; ____ Statisk 96 timers LC50 for fisk (Rainbow trout (Oncorhyncus mykiss)) = 1300 mg/1; Statisk 48 timers LC50 for daphnia (Water flea (Daphnia magna)) * 1600 mg/1 (1300-2100 mg/1); Statisk 48 timers LC50, Amfipode (Scud (Gammarus fasciatus)) = 1100 mg/1 (840-1300 mg/1); 48 timers EC50, Eastern Oyster embro-larvae (Crassostrea virginica)' = 47 mg/1 (10-234 mg/1); 48 timers LC50, Common mummichog (Fundulus heteroclitus) = 2500 mg/1 (1700-3600 mg/1); 1)1)074/ L9a9b3.1r2e-f2.no.: Page 28 of 31 FC-203 LIGHT WATER BRAND AQUEOOS FILM FORMING FOAM (360-710 mg/1); IC50 Respirasjon, Activated sludge * > 1000 mg/1; TTC (2,5,5-trifenyltetrazoliumklorid) test viste ingen ensymhemning for konsentrasjoner opp ~il 1000 mg/1. Sorg for avfallshandtering iht. gjeldende forskrifter. 13 FJSRNING AV KJEMIKALISAVFALL. Metoder for fjerning av kjemikalieavfall: Spesialavfail. Lever avfall til en offentlig godkjent innsamler, mottaks- eller behandlingsanlegg for spesialavfail. Anbefalte avfallsgrupper: HOVSDKATEGORI 07: AVFALL FRA ORGANISKE PROSESSER. UNDSRKATEGORI 07 06: Avfall fra PSD3 av fettstoffer, spe, rengjeringsmidler, desinfeksjonsmidler og kosmetikk. EAX-kode 07 06 03: Halogenerte organise lesemidler, vaskevasker og moderlut. Avfallstoffr.ununer: 7151 Organisk avfall ned halogen. Spesielle konponenter: ir.neholder flourforbindeiser. 14 OPPLYSNNGSR OM TRANSPORT. ADR-klasse: siffer: bokstav: margnummer: CEFIC-kort: UN-nummer: ICAO-klasse : IMDG-klasse: IMDG-side: EMS-nr.: MFAG: O L99a3b1.r2e-f2.no.: Page 29 o f 31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Gvrige opplysninger: Ikke transportsarlig gods. 15 OPPLYSNINGER OM LOVER OG FORSKRIFTER. Merkeetikett: Symbol(er): HSLSESKADELIG (Xn) Inneholder: 2-{2-butoksyetoksy)-etanol 35% Etylenglykol 20% R-setr.inger: Farlig ved svelging. Kan irritere aynene og huden. S-setninger: Unnga innanding av damp/spraytetake. Unng kontakt med huden og aynene. Far man stoffet i aynene; skyll straks grundig med stre mengder vann og kontakt lege. Etter hudkontakt, vask-med spe og vann. Ved svelging, kontakt lege omg&ende og vis denne beholderen eller etiketten. Kun til industriell bruk. Ikke beregnet til bruk som medisir.sk utstyr eller legemiddel. YL-gruppe: 00 Korr, yrkeshyg. luftbehov: Ovrige opplysninger om merkeetiketten: Ovennevnte merking er basert pa tidligere merkeforskrifter og vil v;ere k finne p produktet en tid fremover. Merkingen vil imidlertid erstattes av felgende merking, som er basert p reviderte merkeforskrifter av januar 1998. Symbol: Ingen Inneholder: 2-(2-3utoksyetoksy)-etanol, 1,2-etandiol Kan irritere aynene og huden. Unngi inninding av damp/spraytetike. Unnga kontakt med huden og aynene. Fr man stoffet i aynene; skyll straks grundig med stre mengder vann og kontakt lege. Fir man S t o f f p huden, vaskes straks med stre mengder sipe og vann. Ved svelging, drikk to glass vann og kontakt lege umiddelbart. YL-gruppe: 00 000749 L9a9b3.1r2e-f2.no.: Page 30 of31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM Spesielle lover og bestemmelser: 1. Forskrift on utarbeidelse og distribusjon av Kelse-, Miljo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift out klassifisering og merking av farlige kjemikalier. 3. Forskrift on klassif isering, merking m.v. av stoff som kan medfore miljofare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfall. 6. Etc. 16 ANDRE OPPLYSNINGER AV BETYDNING FOR BRUKERNES SIKKSRHST OG HELSS. Anvendelsesomrider: Filmdannende skumkonsentrat for slukking av brann i hydrokarboner. Viktigste kilde(r) ved utarbeidelsen av KMS-databladet: Datablad fra 3M General Offices, St. Paul, Minnesota, USA. Arsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og KMS-databladforskrifter, fastsatt i januar 1993. Etc-. Egne opplysnir.ger: Informasjonen p dette KMS-databladet er i henhold til vire nvrende opplysninger og beste oppfatning angiende riktig bruk og hindteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medforer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerer.s ar.svar. 000 7iS0 L9a93b1.r2e-f2.no.: Page 31 o f 31 FC-203 LIGHT WATER BRAND AQUEOUS FILM FORMING FOAM FOLGENDE PRODUKTES DEKXES AV DETTE HMS-DATA3LADET : 3M ID-nr. : XF-0008-0217-3 3M AFFF FC-203 Brannslukningss kum Produksjon 3M ID-nr. : XF-0008-0099-5 3M Light Water 3% AFFF Fire Fighting Foam, FC-203,1000 L 3M ID-nr. : XF-0008-0070-6 3M Light Water 3% AFFF Fire Fighting Foam, FC-203, 200 L- 3M ID-nr. ! III I I II I I I Ii i I i : XF-0008-0069-8 3M Light Water 3% AFFF Fire Fighting Foam, FC-203, 20 L I I I II 000^51 i: !i