Document jm03jQEDm9mK9K0vDabEYgq8k
48-HR 94-X (Li
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA)
TEST SUBSTANCE
Identity:PerfluorooctanesulfonaLtiet,hiumsaltm;ay alsobe referretdo as PFOS LisaltF,C-94,orFC-94-X. (1-Octanesulfonaicid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecalfiltuhoiruosm-a,lt, CAS # 29457-72-5)
Remarks: The testsample isa mixtureofthetestsubstanceinwater (approximatel2y4.5% testsubstanceand 75.5% water).No calculations were made to adjustfortheactualconcentratioonfthetestsubstancein the testsample-.
METHOD
Method: Not noted. Test type:Staticacute GLP: No Year Completed: 1994 Species:Daphniamagna Analyticalmonitoring:pH and DO content Statisticamlethods: EC50valuescalculateudsingTrimmed Spearman-
Karber method Test daphnid source: Obtainedfrom U.S. EPA-NETAC, Duluth, Minnesota. Test daphnid age at study initiation<: 24-hours
Test conditions
Dilutionwater.-Carbon-filterweedllwater
Dilutionwater chemistry:
pH:
8.4
DO:
8.6 mg/L
Stock and testsolutionspreparation:A primarystocksolution
was preparedindilutiownatertoyielda testsample concentration
of 1000 mg/L. Alltestsolutio'nwsere made by dilutintghe
appropriataemount ofstocksolutiownithdilutiownaterto make 50
mL of solutionper concentratio.n. Exposure vessels:100 mL glass beakers containing50 mL oftest
solution.
Number of replicates4:
Number of daphnids per replicate5:
Number of concentrations:fiveplusa negativecontrol
Water chemistry during the study:
Dissolved oxygen attesttermination:
7.0 mg/L (controelxposure)
7.8 mg/L (1000mg/L exposure) pH attesttermination:
8.6 (controelxposure) 8.6 (1000mg/L exposure) Test temperaturerange (0- 48 hours) 20.1-2l.O."C Element basis:mortalitaynd immobilization
RESULTS
Nominal concentrations:Bk control1,00,180,320, 560,1000 mg/L Elementvalue:. 24-hourEC50 = 330 (290-370)mg/L
48-hourEC50 = 210 (190-230)mg/L 48-hourNOEC = 100 mg/L StatisticaElvaluation:The EC50 valuesand 95% confidenceintervals were calculatedusingtheTrimmed Spearman-Karber method withtrimset to 0%. Mortalitoyf controls:None Remarks: Values reportedareforthetestsample. No calculations were made to adjustfortheconcentratioonfthetestsubstanceinthetest sample.
CONCLUSIONS
The testsample containing24.5% PerfluorooctanesulfonatLei,thiumsalt exhibitead 48-hourEC5oforDaphnia magna of210 mg/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O.,Box 33331, St.Paul,Minnesota,55133
,DATA QUALITY
ReliabilityK:limischranking2. Thisstudy,whilewellconducted,lacks analyticadlatafor determinatioonfthetestsubstance concentratioinnthe testsolutionsa;nd determinatioonfthe sample purity.
REFERENCES
Thisstudywas conductedby the3M Company, Environmental Laboratory,Lab Request number M1018, 2/10/94.
OTHER
Last changed: 5/2/00
TYPE TEST.
sTAmc
TEST SUBSTANCE.-
ENVIRONMENTAL LABORATORY 48-HRACUTE TO)ACITYTO DAPHNIA,DAPHNIA MAGNA
STATIC RENEWAL
LR M ic)ir
DILUMONWATER-.
CARBON-RLTERED WELL WATER (3M WELL OZ ST. PAUL, MN) TEMP. tcktOC, pH ?,.,A D.O. %.16mgtl
TEST DAPHNID SOURCE-
U.S.EPA-NETAC, Duluth,Mn
DATE RECEIVED-. Co
C\Q
DAPHBOD AGE.* c24 MR OLD NEONATES
NO. OF DAPHNIDSITEST CHAMBER:
5
LOADING
RATE-'
TESTSOLunON
Cil-k DATE lNfflATED:.;@L-?,-
VOLUME.-
60 rnL sra OF TEST VESSELS:
100 mL
DATE TERMINATED-. 'Q-tO-CM IL IH;,g-
TEST" VESSEL
SK1 MQ SK3 SK4
INITIAL TOTAL CONC. AMOUNT mg/L ADDED* CONTROL CONTROL CONTROL CONTROL
MORTALITY
24-HR t
48-HR
PH
I
01 0
0.
0
0
D.O. I mg/L
1.1
IQCJ
0
1.2
1.3
1.4
2.1 %%C) 2.2 2.3 2.4
-SaQ
Cr,'A
cr,
3.2
LA
&3
&4
4.1 SCOO 4.2 4.3 4.4
% It-aL
COMMENTS ANALYST.-
5.1 %cx@l@ zoo 5.2 &3 &4
TEMP. OC IDATE / ANALYST a-ct-qLA ECSO (96% C.L)mg/L
0 C)o cgsIL lettCN,.rr-\c
t@--
C-Ot\C.-
16z\C\ . w C@Z
ENVIRONMENTAL LABORATORY
LFLDate: Analyst:
MIOIS 2/8/94
JBD
Daphnis magns 48-hour ToxicityTest Data Summary
SAMPLE: FC-94-X
SampleConc. (mg/L) 100 180 320 560 1000
No.Organisms Emosed 20 20 20 20 20
% lmmobirmed 0
24 hours
48 hours.
0
0
0
25
45
100
100
100
100
100
24'h ECSO (95% C.I.)= 48 h EC50 (95% C.I.)=
330 mg/L (290-370) 210 mg/L (190-230)
ECSO Estimation Method:
Tdmmed Spearman-Karber 24 h: 0% trim 48 h: 0% trim
<1 ee"v
I..-,:RLINGTONRESEARCH. IN-
TRIMMED SPEARMAN-KARBER'@ETluOD
FOR CALCULATION OF
Et'-'50AND LC50 VALUES IN BI.3ASSAYS
.FOR REF;=RENCE. CITE
M.A. HAMILTON. r@%.C. RUSSO, AND R.V. '@-HURSTON.
TRIMMED-SPEARMAN-KARBER
METHOD * FOR ESTIMATING
LETHAL CONC@-'NTRATIONS IN TO'A'@l,--ITYBIOASSAYS.
ENVIRON'. SCI. TECHNOL. llC7) 714-719
CORRECTIBN 12(4) 417 (11?78).
1977. t4EDIAN
DP-TE TEST
CHEi'o,1 CAL
-2/.9/94 -M 1.n_I-a
FC-9-"t-vA
SPEC IES
0. "ona
DURATION
24-'hours!
RAW DA"'t-A
CONCEN'IRATICN(ma-/L)
100.00 l@M0.00 320.00 560.00 1000.00
NUMBER EXPOSED
20
20
20
2r@,
-20
MORTALITIES
0
0
9
20
20
SPEARMAN-KARBER
TRIM
0.0;)
SPEARMAN-KARBER
ESTIMATES
eC50
32t@.9162.@C.92
95% LOWER CONFIDENCE
289.02
95% UPPER CONFIDE@.ICE
372.05
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QURLINGTON RESEARCH..INC,
Trimmed Soearman-Karber Method for Calculati..jn ai EC50 and LC50 Values in Bioassays
E.
x
x
6.044E .3 x
N
x
.3
a 4.3,A'7E
N
.1
0 2.:@@!90E .3
1.726E
3634E 3
ODODDDEDDDDDDDDEDDDDDDDDDDDDDDE.DDDDDODDVDDDVDEDDDDDD,T)ODDDDDDE
LCIO
LC25
LC50
LC75
Ltr"100
MORTALITIES
rc
-EST CHEMICAL
2/8/94 M.1ole FC-94-X
SPECIES
0. macna.
DURATION
.4e@---hours-i
RAW DATA
CONCENTRATION(ma./L)
4L(."i,"",.00
120.00
320.00 560.00 1000.00
NUMBER EXPOSED
20
MORTAL-JTTIES
0
SPEARMAN-KARB=-R
TRIM
20.
20
20
20
5
.20
--0
20
.0.0c)
SPEARMAN-KARBER
EST'LMATr@-S
ec!-:.o
207.5236053
95% LOWER CONFIDEN"-'E
185.42
95% UPPER CONIFIDENCE
232.26
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