Document jjNEobyOw17JQJ26MDbB8Kq9

PFOA | 11113399..00000011 Exhibit 1139 I3MM_MMNND011669900337700 Industrial 1B 810 -V FRTOENO STATGELa2bOoAraDtovies, Inc. NORT1H81B0ROFORKO.NITLALGIENORIOSAD 60062 NORTHBROOK. tLLINOIS 60062 RREEPPOORRTT TTOO 33MM CCOOMMPPAANNYY 2268--DDAAYY OORRAALL TTFOOCXX-II1CC4II3TTYY SSTTUUDDYY WWIITTHH IINN AALLFBBCII-NN1O4O3MMIICCEE IIBBTT NNOO.. 88553322--1100585555 11113399..00000022 3M_MND1620371 3M MN01690371 Induitial BIO TEST Lokoratorist, Jue. ) RREEPPOORRTT TTOO 33MM CCOOEM~PPAANNYY 228--DDAAYY OORRAALL TTFOOCXX-II1CC4II3TTYY SSTTUUDDYY WWIITTHH iINN AALLFBBCII-NN1OO43IMMIICCE IIBBTT NNOO.. 88553322--1100665555 II.. IInnttrroodduuccttiioonn AA mmaatteerriiaall iiddeennttiiffiieedd 2ass FFCC--114433 ((TT--11774422CCOcCC,, LLoott NNoo.. Z26699)) wwaass oobbttaaiinneedd ffrroomm 33MM CCoommppaannyy ffoorr tthhee ppuurrppoossee ooff ccoonndduuccttiinnggaa 2288--ddaayy ppiilloott ffeeeeddiinngg ssttuuddyy iinn aallbbiinnoo mmiiccee ttoo eessttaabblliisshh ffeeeeddiinnEg lleevveellss ffoorr lloonnEg--tteerrmm ssttuuddiieess iinn tthhaatt ssppeecciieess.. TThhiiss rreeppoorrtt pprreesseennttss tthhee rreessuullttss ooff tthhee eexxppeerriimmeenntt.. 11113399..00000033 33MM_MMNND011669900337722 Incishial BIO-TEST Lakoralorist, Ine. 2 ) DTbT.. Summary Summary A 28-day oral toxicity study was conducted in which groups of albino A 28-day oral toxicity study was conducted in which groups of albino mice were fed dietary concentrations of 0, 30, 100, 300, 1,000, 3,000, 10,000, mice were fed dietary concentrations of 0, 30, 100, 300, 1,000, 3,000, I0,000, or 30,000 parts of FC-143 per million parts of diet or 30.000 parts of FC-143 per million parts of diet. The results obtained during the investigation revealed reduced body The results obtained during the investiga~on revealed reduced body wweeiigghhtt ggaaiinnss f~oolllloowweedd bbyy lloosssseess iinn bbooddyy wweeiigghhtt aammoonngg aallll tteesstt ggrroouupp aanniimmaallss ((3300,, I10000,, 330000 ppppmm)) tthhaatt wweerree ssaaccrri~fiicceedd aaf~tteerr 2288 ddaayyss oof tteessttiinngg.. AAnn iinnccrreeaassee iinn aavveerraaggee ffoooodd ccoonnssuummppttiioonn wwaass eexxhhiibbiitteedd bb7y aallll aanniimmaallss ffeedd FFCC--114433.. HHoowweevveerr,, eexxcceessssiivvee wwaassttaaggee ooff ffoooodd ffrroomm tthheeiirr ffeeeeddeerrss mmaakkeess iinntteerrpprreett3a.ttiioonn ooff tthhiiss ppaarraammeetteerr ddiiffffiiccuulltt.. AAllll mmaalleess aanndd ffeemmaalleess ffeedd 11,,000000 ppppmm oorr mmoorree ooff FFCC--114433 ddiieedd ~witthhiinn tthhee f~iirrsstt 99 ddaayyss oof tteessttiinngg.. AAllll TT--TITIT ((330000 ppppmm)) mmiiccee eexxcceepptt 1I mmaallee ddiieedd within 2266 ddaayyss ooff tteessttiinngg.. OOnnee aanniimmaall ddiieedd iinn eeaacchh ooff tthhee 3300 aanndd 1I0000 ppppmm tteesstt ggrroouuppss.. NNoo ootthheerr ddeeaatthhss ooccccuurrrreedd.. RReeaaccttiioonnss nnootteedd dduurriinnEg tthhee iinnvveesst~iggaattiioonn iinncclluuddeedd rroouugghheedd ffuurr aanndd mmuussccuullaarr wweeaakknneessss ffoorr aallll aanniimmaallss ffeedd 33,,000000 ppppmm oorr ggrreeaatteerr aafftteerr tthhee ffiirrsstt d4 ddaayyss ooff tteessttiinngg.]. AAnniimmaallss ffeedd 11,,000000 ppppmm ((TT--IIVV)) ooff FFCC--114433 eexxhhiibbiitteedd ssiimmiillaarr rreeaaccttiioonnss aanndd ssiiggnnss ooff ccyyaannoossiiss aafftteerr 66 ddaayyss ooff tteessttiinngg.. AAllll ooff tthheessee rreeaaccttiioonnss wweerree eevviiddeenntt iinn tthhee 330000 ppppmm ((TT--TUTDT)) ggrroouuppss aafftteerr tteesstt ddaayy 99.. SSoommee TT--TITI ((1i0000 ppppmm)) aanniimmaallss eexxhhiibbiitteedd sslliigghhtt ccyyaannoossiiss oonn ddaayyss 1100 aanndd 1Ii1 ooff tteessttiinngg,, bbuutt aappppeeaarreedd nnoorrmmaall ffoorr tthhee rreemmaaiinnddeerr ooff tthhee ssttuuddyy.. A,%l1l1 3300 ppppmm ((TT--II)} aanniimmaallss rreeaacctteedd ssiimmiillaarr ttoo tthhee ccoonnttrroollss dduurriin=gg tthhee Z288 ddaayyss ooff tteessttiinngg.. 11113399..00000044 I3MM_MMNND011669900337733 Judustiol BIO TESTLoboralories,Inc. 3 Gross pathologic examination of all animals sacrificed after 28 days of Gross pathologic examination of a~l animals sacrificed after 28 days of testing revealed enlargement and/or discoloration of 1 or more liver lobules. tes~:ing revealed erdargement and/or d~scoloration of 1 or more liver lobules. Statistical analyses revealed significant increases in the absolute liver Statistical analyses revealed significant increases in the absolute liver weights from males and females of the remaining 30 ppm (T-D), and weights from males and females of the remaining 30 ppm (T-I), and 100 ppm (T-II) test groups. Increases were also noted in the liver to 100 ppm (T-I!) test groups. Increases were also noted in the liver to body weight ratios for these animals. body weight ~'at~os for these animals. Histopathologic examination of all sacrificed mice revealed treatment-related Histopathologic examination of a]! sacrificed mice revealed treatment-related liver findings in the males and females of all test groups examined, 30 ppm liver findings in the males and females of all test groups examined, 30 ppm ((TT-DI)),, 1i0000 ppppmm ((TT-IID!)),, aanndd iinn ttwwoo i1,,000000 ppppmm ((TT--IIVV)) aanniimmaallss tthhaatt ddiieedd dduurriinngg tthhee ssttuuddyy.. TThheessee ffiinnddiinnggss ccoonnssiisstteedd ooff ddiiffffuussee ccyyttooppllaassmmiicc eennllaarrggeemmeenntt ((hhyyppeerrttrroopphhyy)) ooff hheeppaattooccyytteess tthhrroouugghhoouutt tthhee eennttiirree lliivveerr lloobbuul!eess ((ppaannlloobbuullaarr)) aaccccoommppaanniieedd bbyy ffooccaall ttoo mmuullttiiffooccaall ccyyttooppllaassmmiicc lliippiidd vvaaccuuoolleess.. TThheerree wwaass aallssoo ddeeggeenneerraattiioonn aanndd//oorr nneeccrroossiiss ooff hheeppaattooccyytteess aanndd ffooccaall bbiillee dduucctt pprroolliiffeerraattiioonn aammoonngg aanniimmaallss ooff aallll tteesstt ggrroouuppss.. J) 11113399..00000055 IM_MND1690374 3M MN01690374 Industrial BIO TEST Lokoraloriss, Inc. 4 The The oiher other Liver liver cchhaannggeess oobbsseerrvveedd wweerree tthhoossee ooff nnaattuurraallllyy ooccccuurrrriinngg disease or related to the method of sacrifice. disease or related to the method of sacrifice. Respeciilly submitied, INDUSTRIAL BIO-TEST LABORATORIES, INC. INDUSTRIAL BIO-TEST LABORATORIES INC. Report prepares vy. BomrinCMon Bke, Report prepared by: BABnooinrmnaaisel GCCrhuraoituseptrooTppehhceehrrnician RAondiemanlt Tosicity Group Technician Rodent Toxicity bG~roAdupnuLdeJT ar .ndeM erse wt~g5.rSiiea~ . RGordoeunpt LTeoasdiecrity Rodent Toxicity RReeppoorrtt approved approved bbyy:: Loe. ol, 0). Ladd Acting DDeennnniiss SWWe.c. tAiAorrnnnooHlledda,,d,BB..TSSo..xicology py Acting Section Head, . Toxicology renee Kooe KotiKoTodb - Technical Manager ToxiPcho.lDo.gy Technical Manager, Toxicology BVBeiaercreeiieePreMWs.idPPehnitLlRiapnsd, PRD Ph.D. VDiirceectPorresoifdeRnetseaanrdch D~ector of Research QDuaanlaitVyerAifsiweadrabnyce Unit Quality Assurance Unit Fo Assurance S&u~pervise a Q~lity Assurance Supervisor 11113399..00000066 S_MNotes037S 3M MN01690375 Industrial BIO TEST Laboraloriot, Inc. 5 o.Ud. PPrroocceedduurree AA tt~ottaall ooff 8800 yyoouunngg ((4433 ddaayy oolldd)) CChhaarrlleess RRiivveerr CCDD aallbbiinnoo mmiiccee**,, 4400 of each sex, was randomly divided into groups of 5 male and 5 female animals each sex, was randomly divided int~ 8 groups of 5 male and 5 female animals cach. The animals were allowed 3n 8 day pre-test period to adjust to the each. The animals were allowed an 8 day pre-test period to adjust to the caging system. Groups were fed 0 (Control, 30, 100, 300, 1,000, 3,000, caging system. Groups were fed 0 (Control), 30, i00, 300, 1,0000 3,000, 10,000, ox 30,000 parts of FC-143 per million parts of diets. i0,000, or 30,000 parts of FC-143 per million parts of diet~*. Al animale were individually housed with food and water available sd All animals were individually housed with food and water available ad Mbitum. Daily observations for behavioral reactions or mortalities were made. libit~m. Daffy observations for behavioral reactions or mortalities were made. Animals were weighed and food consumption calculations were made weekly Animals were weighed and food consumption calcu!ations were made weekly for a 28-day period. for a 28-day period. At the final sacrifice, a complete gross pathologic examination was conducted At the final sacri~ce, a complete gross pathologic examination was conducted on al surviving mice. Animals that died during the investigation were also on all su.rviving mice. Animals that died during the investigation were a!so ! eexxaammiinneedd ggrroossssllyy.. AAtt tthhee ttiimmee ooff ggrroossss eexxaammiinnaattiioonn,, aa rreepprreesseennttaattiivvee sseett ooff argans snd other sssues was removed and preserved in neutral buffered organs and other tissues was removed and preserved in neutral bu~ered ffoorrmmaalliinn ffoorr ffuuttuurree hhiisstwoppaatthhoollooggiicc eexxaammiinnaattiioonn.. TThhee wweeiigghhtt ooff tthhee lliivveerr ooff each sucriiced mouse wis determined and liver to body weight ratios were each sacrificed mouse was determined a~d liver to body weight ratios were calculated. calculated. Microscopic 'Microscopic eexxaammiinnaattiioonn wwaass ccoonndduucctteedd oonn tthhee lliivveerrss frroomm al sacrificed mice. sacrificed mice. AAllssoo aatt ssaaccrriiffiiccee,, 5 anld ooff ssooddiiuumm hheeppaarriinniizzeedd bblloooodd ((wwhheenn ppoossssiibbllee)) aanndd 10 I0 g of of whole whole Liver Liver (pooled) (pooled) from from the the Control Control ((00 ppppmm)),, TT--I1 (3300 ppppmm)),, aanndd TIL T-If (100 (I00 ppm) ppm) groups groups wweerree ccooll!leecctteedd aanndd qquuiicckk ffrroozseenn.. TThhee ssaammpplleess wweerree subaitied to 3 Company for analyses. submittal ~o 3M Company for analyses. + Charles River Breeding Laboratories, Wilmington, Mass. * PSPCuuhtrrairiLlnnoeaausiRRsaaR,ttivMCCeirhhsoosBwwor,u,erei((d.ddirrnyyg,, Lppauubllovvreearrtiiozzreieedds.ddiieeWtti))l,,miRRnaaglltssottnoo,nn--M PPuuarrsiisnn.aa CCoommppaannyy,, St. Louis, Missouri. 11113399..00000077 S_MNotes03TS 3M MN01690376 Inlushial BIO -TEST Lakorsioris, Inc. 6 \ IV. Results IV. Results A. Body Weights A. Body Weights M Meeaann mmaallee aanndd ffeemmaallee bbooddyy wweeiigghhtt ddaattaa ccoolllleecctteedd dduurriinngg tthhee iinnvveessttiiggaattiioonn aarree pprreesseenntteedd ii~n TTaabbllee II.. AAl1l1 tteesstt ggrroouupp aanniimmaallss lloosstt wweeiigghhtt.. AAnniimmaallss ffeedd 3300,, i10000,, oorr 330000 ppppmm ooff FFCC--114433 eexxhhiibbiitteedd rreedduuccttiioonnss iinn bbooddyy wweeiigghhtt ggaaiinnss ffoolllloowweedd bbyy lloosssseess iinn bbooddyy wweeiigghhtt dduuar-iinngg tthhee iinnvveessttiiggaattiioonn.. TThhee bbooddyy wweeiigghhttss wweezr-ee ddeepplr-eesssseedd iinn aa ddoossee--~r-eellaatteedd mmaannnneerr.. 11113399..00000088 I3MM_MMNND011669900337777 Instastial BVO -TEST Lakonalovist, Inc 7 ) TTAABBLLEE I TTEESSTT MMAATTEERRIIAALL:: FFCC--114433 2288--DDaayy OOrra~l! TTooxxiicciittyy SSttuuddyy -- AAllbbiinnoo M Miiccee BBooddyy W Weeiigghhtt DDaattaa SSuummmmaarryy ooff MMeeaann VVaalluueess "Grou ~ and Group Dieatnadry Body Weight Tota44l--W WWeeeeeikkght DLieevtaerly Week: Body Weight Change Total Weight (ppm) Sex 0 1 2 3 4 Level Week: (ppm) Sex 0 1 2 3 4 (g/mouse) Change (~/mouse) CoCnonttrrooll MFM233333 2304 2m 357 B235 424080 +v577 (0) F 23 24 25 24 28 + 5 aTT-I0I FMM o 333 2333 33ou4 32m 2 na32e "--12i (30) F 23 24 25 22 21"* - 2 TT0-0nH) MFM 2333333 222188*% 2gl5es*em*w 22L33e*wm* s125em*x* --- 868 (I00) F 23 Zl 18"* 16"* 17"* - 6 TT-Imll (300) MM 3333 F233 22a33m*me* 2 a23r*e* 22I1ra"em* 2200-*m* 1--133 (300) F 23 17"* 17"* 17" - - Tv a(l.T,0-OI0OV0O)) M3 ae FMFo le 33 21"* 23 14"* -- - -- - Tv Moo =--- - ((33T.,-0000V00)) FMFoo 33 23 - - - - - oTTo-IVmI) MFM223 333 .-- --- --- - - (I0,000) F 23 - - - GTTo-voVH) FMM o 32333 = - - - - - (30,000) F 23 SFatsteallsyignificant mtergroup difference at the 95percent (B< 0.05) * cSotnatiisdtiecnaclelylesvigen.lificant intergroup difference at the 95 percent (P < 0.05) =** SccStootaanntftfiiiissddtteieicncnaacclleellyylleessviivggeennlli.iffiiccaanntt iinntteerrggrroouupp ddiiffffeerreennccee aatt tthhee 9999 ppeerrcceenntt ((P2 << 00..0011)) J - =cAolnlfidaneinmcaelslevdieel.d. - = All animals died. 11113399..00000099 33MM_MMNND011669900337788 Inlustiol B10 ~TEST Laboralorias, Inc. 8 ) B. Food Consumption B. Food, Consumption Mean weekly food consumption data collected during the investigation Mean weekly food consumption data col3ected du~'ing the investigation are presented in Table I. All surviving test group animals exhibited an are presented ~ Table H. ~ s~n~ test ~roup ~m~s e~bited ~ increase in the average food consumption when compared to controls. ~crease ~ ~e av~a~e f~d ons~p~on when compared to consols. However, in each of the groups fed FC-143, excessive food wastage was However, ~ ea~ o the ~oups fed FC-143, excessive ~ood w~taEe was observed during the study. observed d~g ~e s~dy. J 11113399..00001100 33MM_MMNND011669900337799 Industrial BIO -TEST Laboratori, Ine. 9 GGarrnooduupp Dieatnadry DoLLieeemvtvae)erlly Sex (ppm) Sex CCoonnttrrooll MFM (0) F TaT--n1I ONMF (30) F T T-U ONM a(io00)) FF TT-Imll MOM [(3E00N) F aTTs-IvVm FMM (I ,000) F GTT0-V0V ) (3,000) FMFou 0.TV-0V0II0 MFM (I0,000) F T T -VlE l MM (00o,,o0o00o)) FFE TTAABBLLEE H1 TTEESSTT MMAATTEERRIIAALL:: FFCC--114433 2288--DDaayy OOrraall TTooxxiiciitDy, SSttuuddyy -- AAllbbiinnoo MMiiccee FFoooodd CCoonnssuummppltiioonn DDaa~taa FF(oogoo/ddmoCCuosonens/su7ummpdptatiyisoo)nn 1 (E/mouse/7 2Week: Week: days) 3 4 I 2 3 4 AACvovenersraauggmeeptFFiooooondd ((sg//Cmmooonuusssueem//77ptiddoaanyyss)) NNNAAA 24400 1ii200 5s5e74 85680 NA 42 52 57 50 NNNAAA o88m4a 111s881 8899 "9977 NA 87 57 76 73 NNAA Na 566 a 113m 66 77766 8829 NA 63 77 77 7Z NNAA NA 112210 1m 11493% 3mI0-1 11-2222 NA 133 96 - - NNNAA A.-.. -- . -- .-- NA .... - --- - - --- - -- -- -- -- -- - --- - - --- - - = All animals died. N-A== DAaltl aanniomtaalsvaidliaebdl.e. Food consumption inadvertently collected as a NA = mDmeeaaatann nppoeetrragvgrraooiluuappbleffo.orr mmFaaolloeedss caaonnnddsuffmeempmatailoleenss ittnooaggdeettvhheeerrrt.e.ntly collected as a 11113399..00001111 I3MM_MMNND011669900338800 Indactial BIO TESTLaboratori,Ince 10 \ CC.. MMoorrttaalliittyy aanndd RReeaaccttiioonnss AAlDl aanniimmaallss iinn tthhee TT--IIVV ((i1,,000000 ppppmm)),, TT--VV ((33,,000000 ppppmm)),, TT--VVII ((I100,,000000 ppppmm)),. sanndd TT--VVIIII ((3300,,000000 ppppmm)) ggrroouuppss ddiieedd wwiitth}dinn tthhee ffiirrsstt 99 ddaayyss ooff tteessttiinngg.. AAllll mmiiccee,, bbuutt 11 TT--IIIIlI ((330000 ppppmm)) animal animal ((mmaallee NNoo.. 3311)),, ddiieedd wwiitthhiinn 2266 ddaayyss ooff tteessttiinngg.. OOnnee aanniimmaall ddiieedd iinn eeaacchh ooff tthhee 3300 aanndd 110000 ppppmm tteesstt ggrroouuppss dduurriinn~g tthhee 2288 ddaayy ffeeeeddiinngg ppeerriioodd.. NNoo ootthheerr ddeeaatthhss ooccccuurrrreedd dduurriinngg tthhee ssttuuddyy.. RReeaaccttiioonnss nnootteedd dduurriinngg tthhee ssttuuddyy iinncclluuddeedd rroouugghheedd ffuurr aanndd mmuussccuullaarr wweeaakknneessss ffoorr aallll aanniimmaallss ffeedd 33,,000000 ppppmm oorr mmoorree aafftteerr tthhee ffiirrsstt 44 ddaayyss oof tteessttiinngR,. AAnniimmaallss ffeedd !1,,000000 ppppmm ((TT--IIVV)) ooff FFCC--114433 eexxhhiibbiitteedd ssiimmiillaarr rreeaaccttiioonnss aanndd ssiigEnnss ooff ccyy~annoossiiss aafftteerr 66 ddaayyss ooff tteessttiinngg.. AAllll ooff tthheessee rreeaaccttiioonnss wweerree eevviiddeenntt iinn tthhee 330000 ppppmm ((TT--IITIT)) gSrtoouupp aafftteerr 99 ddaayyss ooff tteessttiinngg.. SSoommee 110000 ppppmm ((TT-T+T)) aanniimmaallss eexxhhiibbiitteedd ssl]iigghhtt ssiiggnnss ooff ccyyaannoossiiss oonn ddaayyss 1100 aanndd i1i1,, bbuutt aappppeeaarreedd nnoorrmmaall ffoorr tthhee rreemmaaiinnddeerr ooff tthhee sSttuuddyy.. AAllll 3300 ppppmm ((TT--II)) aanniimmaallss aappppeeaarreedd ssiimmiillaarr ttoo tthhoossee ooff tthhee ccoonnttrroollss.. NNoo ootthheerr uunnuussuuaall bbeehhaavviioorraall rreeaaccttiioonnss wweerree nnootteedd.. DD.. PPaatthhoollooggiicc SSttuuddiieess 1. Gross Pathologic Findings i. Gross PatholoEic Findings GGrroossss ppaatthhoollooggiicc eexxaammiinnaattiioonn ooff aanniimmaallss ssaaccrriiffiicceedd aafftteerr 2288 ddaayyss ooff tteessttiinngg rreevveeaalleedd eennllaarrgEeemmeenntt aanndd//oorr ddi~ssccoolloorraatti[oonn ooff 11 oorr mmoorree lloobbeess ooff tthhee lLiivveerrss ooff aallll tteesstt ggrroouupp mmaalleess aanndd ffeemmaalleess.. NNoo ootthheerr ggrroossss ppaatthhoollooggiicc d,i~ffeerreenncceess bbeettwweeee-nn tthhee tteesstt aanndd ccoonnttrrooll aanniimmaallss wweerree nnootteedd.. 11113399..00001122 33MM_MMNNO011669900338811 ' Sdaatial BIO TESTLaboratonst, Su. un \ 2. Liver Weight and Liver to Body Weight Ratio Data 2. Liver Weight and Liver to Body Weight Ra~io Data The results of the statistical analyses conducted on the sbsalute The results of the s~atisrical analyses conducted on the absolute liver weights and liver to body weight ratios are summarized in Table II. liver weights and liver to body weight ratios are summarized in Table LII. Significant differences between a test group and a control group axe Significant di/~erences betv~een a test group and a control group are ddeessiiggnnaatteedd bbyy aasstteerriisskkss.. SSttaattiissttiiccaallllyy ssiiggnniiffiiccaanntt iinnccrreeaasseess wweerree nnootteedd iinn the absolute liver weights among the males and females of the remaining test the absolute liver weights among the males and females of the remaining test groups (30 and 100 ppm). The increased liver to body weight ratios for groups (30 and 100 ppm). The increased liver to body weight ratios for these animals can be atributed to the reduced body weights after 26 days these animals can be arn-ibuted to the reduced body weights after 28 days of feeding. of feeding. J 11113399..00001133 W3M_MMNN001166090338822 Industrial BIO TESTLbosatontit,Joe. ! ig 311: fi (30% 1p: oiud iz, eo LEE 2 i i, 13s | tii i23.p1 ni. ss STEERIREREL CEs iioiE ii: wEEo ofi oLl iLte to.l igd 2 PE: R2 EEE| 2 EiE 3: iRo5 iE iIiF3 zog EE z | i; PEREE IS 2g 32 ii2 : 0% ii [iEI5E53E. 1{57574 320 E3NE 3aIE3F 133 2 F 2ijzE sz3E z%E| i233i5ARiE 5 HI i230 P| oflleBE. zzyiigE8d3 POE BIT Ijtipe aW_MND1650383 3M MN01690383 11113399..00001144 Indistial BVO TESTLaboratories,Ine. 113B ) 3. Histopathologic Findings 3. Histopathologic Findings Histopathologic examination was conducted on sections of livers Histopathologic examination was conducted on sections of livers taken from all mice sacrificed after 28 days of testing and from 2 T-IV. taken from all mice sacr~ced after 28 days o~ testing and from Z T-IV (1,000 ppm) animals that died during the investigation. Treatment-related (l,000 ppm) animals that died during the investigation. Treatment-related liver ndings were noted among the males and females from all test groups liver ~ndings were noted amonE the males and females from all test groups eexxaammiinneedd.. TThheessee fRinn~diinnggss aarree pprreesseenntteedd iinn TTaabbllee IIVV.. TThhee kkeeyy ffoorr tthhee grading system and abbreviations used is shown below: grading system and abbreviations used is shown below: += minimal in severity +++ == mmiilndimian! sinevesreivteyrity +++++ == m moidlderianteseivnersietvyerity ++4+:++t+F++.~ === = moderate in severity fmoacrakled in severity marked in severity MF == mfoulctailfocal MD == dmiuffltuisfoeca/ D = diffuse The pathologist's statement follows on the next page. The pathologist=s statement follows on the next page. 11113399..00001155 I3MM_MMNND011669900338844 Industrial BIO -TEST Laboraloriss,Inc. mrIBT No. 8532-106_55 3M Company 1144 June 9, 1977 bee cto Hepat smi of i int is I have completed a histopathological examination of H~E stained sections of liver from control and test (Groups T-I, T-II, T-III) mice of IBT No. ws. tn sean, ens o re vm sn smd tt $0 8532-i0655. In addition, sections of liver from two anima!s of test group te et toi se ty ae to eto, T-IV that died duxing the study were also examined. ee ttnt rte Shs oo ort i sh mi Treatment-related morphologic changes were present among both male rt fete se oo oe 9 ns cont of on and female animals of all test groups. The lesions consisted of diffuse rem states Goma of 1 sparen arose ios cytoplasmic enlargement (hypertrophy) of the hepatocytes situated tlu~ughout the entire liver lobules (panlobular) accompanied by focrl ro mulrifocal cytoplasmic lipid vacuoles of variable size which were random in distribution. oes oo sn opr ton ste sen at en There was also degeneration and/or necrosis of hepatocTtes and focal bile rasan sors so 2 on duct proliferation among animals of all test groups. stn ee en ser er tons of sti sin The other liver changes observed were lesions of natu.rally occurring tres or ett eae ot in 8 tr se nn diseases or related to the method o sacrifice. ALl findings are tabulated in Table IV. Lpca Vt 5. Arceo, "M.D. Staff Pathologist Reviewed and Approved: Reviewed and Approved: TDms: GtaadleTu C . Ss Donovan E. Gordon, D.V.M., Ph.D. Diplomate0 American College o Veterinary Pathologists 11113399..00001166 3M MN01690385 Indtiol BIO-TEST Laboratori,Inc 115 ) i119 i iiiid ii 45 iis 23 itl EE gf i FjiRtEI FER B3 3301 i1)E : T1i0inda FY 1 i{34 ; HY sree prim 11113399..00001177 awnotesusss 3M MN01690386  0 eduatial BIO-TEST LoboS.rc 3 i31g31 Hi t 31i3t] i [51s H5E i PvEi4LtidE]i Bg81idn, eiURi bk :3E47% Sal i EEEERRErEAiE1 1 . i $3 d 3 eesti ii Bi s #iof iifr 3$43#33i44 oes ii :) iSBisi8f I 1zaE zacE ess I O. ii]::I3 . E 11113399..0000118 1 a3MW_MMNND011665900338877