Document jgg6eyVo3X2epv89a9XYEJgBp

A f L SLSL& _ C Q 2 Z ACU TE TO XICITY TO FISH (FATH EAD MINNOW ) T E ST SU B ST A N C E _______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFO S, FC-95, or as a component of FX-3106. (1Octanesulfonic add) (C A S # 2795-39-3). Remarks: The 3M production lot number was 015G. The test sample is FX-3106, PO # S5233-004, EAI 85548, Sumitomo 3M Japan. Current information indicates it is a mixture of 2.31% PFO S, 2.6% diethylene glycol butyl ether, 83.38% water, 10% ethylene glycol, 1.36% Sultone foamer, 0.2% xanthan gum, 0.1% 2-biphenylol, sodium salt, tetrahydrate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemica! component o f the test sampie. M E T H O D :_______________________________________________________ Method: Not given. Type: Acute static-renewal GLP: No Year completed: 1985 Species: Pimephales promelas Supplier: Fattig Fish Hatchery, Brady, NE. Analytical monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours Statistical methods: Not applicable. No mortality observed. Test fish age: Juveniles Length and weight: Average length - 33 mm Average weight = 0.27 g (wet) Loading: 0.45 g/L Pretreatment: None Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry: Hardness: 260 mg/L as CaCC>3 Alkalinity: 220 mg/L as C a C 0 3 Conductivity: 450 pmhos/cm Lighting: Not given. Stock and test solution preparation: The test material was added directly----- to the dilution water on a weight/volume basis. Concentrations d o sin g rate: Solution renewal during the study. Stability of the test chem ical solutions: Not noted. Exposure vessels: 4 L glass beakers containing 3 L test solution. Number of replicates: 2 003015 Num ber of fish per replicate: 5 Num ber of concentrations: five plus a blank control Water chem istry during the study: pH range (0-96 hours): 7.8 - 8.2 (control exposure) 7.9 - 8.2 (1000 mg/L exposure) Temperature range (0-96 hours): 20 C D issolved oxygen range (0-96 hours): 6.9 - 9.6 mg/L (control exposure) 6.6 - 9.6 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control, 10, 50,100, 500, and 1000 mg/L Element values: 96-hour LCso = >1000 mg/L Element values based on nominal concentrations Rem arks: Testing was conducted on a mixture as described in the Test Substance rRmarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C LU SIO N S____________________________________________ ' The test sample 96-hour LC50 for fathead minnow was determined to be >1000 mg/L, the highest concentration tested. No mortality or sublethal effects were observed in this concentration. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY__________________________________________________ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the study lacks information on purity of the test substance and analytical confirmation of the amount of fluorochemical proportion in solution. R E F E R E N C E S ___________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number C 2 2 8 2 ,1985. O TH ER_________________________________________________________ Last changed: 6/26/00 003016 0UxU 1c ENVIRONMENTAL LABORATORY FISH T O XICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) l r _c <Ma Heuv~ *-3iOfa fbJfc$ $**3-eo* TY P E T E S T Q<.y^ u * S V cX,VlC ------M A T E R IA L T E S T E D 4 1 8SSW SuW, W 3a>J a p a n I OT O t S Cr TES T ORGAN ISM K x+ W A ^ m n o u j S QURCE QcJ!% . ^ S ^ PATE R E C D_ / - j 3 3 ~ g 6 ~ BATCH NO . 3 / j 3A V G . W T .C - 3 7 grams A V G . S I Z E , 3 cm NO. ORGANISMS PER DOSE ^ 0 .HTL OADING FA C T O R c F I S H D I S E A S E T R E A T M E N T ^ / O n * - ________________________________________________________________ 3T E S T S O LU TIO N VOLUME LITERS/ALL-GLASS TANK TANK DIMENSIONS: 4 LIT ER PYREX^GLASS BEAKE TIME STARTED A M DATE STARTED l O ^ H ~ S ~ DATE COMPLETED / / D ILU TIO N WATER. CARBON-FILTERED WELL WATER (TEM P A O (s & . 3 l _ ) C . . D . Q . *? ppm. pH INVESTIGATOR OK H . L C . Q ( 95% C O N F I D E N C E L I M I T S ) . Tank I n i t i a l T o tal 24 H ours I 48 Hours 1 72 Hour 5 ______ 96 Hour __ No. Cone. Amount T o ta l No. pH D.O. T o ta l No. pH D.O. T o t a l No. pH D.O. T o t a l No. pH D.O. mg/1 Added Dead X CONTROL m a * M to 1 30 O ppm 13 D 7-1 7 . Dead 0 ppm 7 1 7.3 7 . Dead O O ppm g.o 7.H g .a 7-1 Dead ppm 1?tMe. 0 ft IZ l So ISO o y>s o tT 7.1 4 . 1 o 8-1 y.to 13 b i - 3 ice 3co o 1 4.7 0 7-1 4 . 1 o M So o iSc o 1-5* loco 3oco 0 ! ii 71 J 7.1 o 7.1 *7} o 1 oo 7.1 7 . 3 8 \ 4.1 8- ^1 IM yq -o o n Tf) tetf Iti (n.Q 13 l 1 T e s t Temp. C SO L. sto \ So I n i t i a l s & Date Jc-'icSS SS io 31-s t 1 COMMENTS: U?A Tn^ia.\ p iwt ChtriiJLl H SA y t V\<a itoJr, W-4I'tciUMyGang. LAgdyj .f C Q .m j L Scio.uj /iieTvT S d R Ib i'.ss $em* 3pl?r-,n.r \ Tank No. Symptoms o r R e actio n s 24-H r. CONTROL M \ /p. \ -3 '*? 1*3 1-4 \ zzzsl-S BEST COPY AVAILABLE VT o ta l Numbe r A ffe c te d 48-H r. 7 2 -H r. ............. \ 96-H r. \ \ ____ c% > Cv \ .x_\ \\ ___________ \ 003017 !\<rke.u:cM ^ > t< v H C L 9 !o -4cu4c TYPE TEST R gn gu ^ a .1 S l a - h p ENVIRONMENTAL LABORATORY FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) lr c a a s a F/-3IOt ?o#S 5233-001 MATERIA! TFSTFnfrAl S5~5Y& S uwiW * 3M J a p a n inTOlfffr TEST ORGANISM^ai-k&ul /Hin^ujSOURCE_^ I S Z J j c a 't i k T i . REC'DJ - < Z 2 > - 8 5 BATCH N0.3 1 % AVG. WT<? ^ 7 . grams AVG. SIZE. 3 . 3 cm NO. ORGANISMS PER DOSE . * T l.OAniNfi FACTORS H.S~ru FISH DISEASE TREATMENT, M o y \<L._____________________________ TEST SOLUTION V0LUME_3j-ITERS/ALL-GLASS TANK TANK DIMENSIONS: 4 LITER PYREX@GLASS BEAKER TIME STARTED, ? - ^ AM DATE STARTED, ( Q - 2 cl'g S ~ DATE COMPLETED / / - & C" DILUTION WATER CARBON-FILTERED WELL WATER (TEMP, A O H . . D.O. 9 k ppm. pH ). INVESn^ ATOR^ tw ^ LCRn(95% CONFIDENCE LIMITS) > / M i ) U & C f Tank I n it ia l Total 24 Hours 48 Hou 'S ______ 72 Hours___ ______ 96 Hours___ No. Cone. Amount Total No, pH D.O. T o ta l No, pH D.O. Total No. PH D.O. T o tal Mo. pH D.O. mg/1 Added Dead ppm Dead ppm Dead ppm Dead ppm I T CONTROL m s ? 2M 10 3o a - a IS O a - } 1CO 3 o o S o o IS o o lo c o 3 o co 0 S o ` 1 &.1 7.1 c 7. a C 3-1 7. A o 23 */ o 3-1 7 ^ D 7 s 7 / D 7-1 7 i 0 . *? 0 7.1 7 6 7.1 7 '6 0 7. ' A O 3.0 7 .0 o C t o 7-1 o o .I 0 Sl -& 0 S3 U o 0 to U n IS 79 la 1 fi 1 ,1 18 1 ,0 IS tp ,P 1 ft o li Test Temp. C Ao <2o ALO Z jO I n it ia ls & Date J - z o - g s S*e> ( o - 3 1 - e s " S - 1 - 8 5 ^ HM VS C O M M EN T S r ^ iv < a u c il lygflhtu_p H f Tev4 cWmiCaA l<> a y# Ucmj Toam v / o a o f i Q - . S .A tv\ to<i*er <l4Tr6<>*V\, > e ^ P ir < y ffr . 003018 2-10-85 ENVIRONMENTAL LABORATORY FIN A L REPORT LAB REQUEST NO. C2466 '.QUESTER NAME: RRR PT: 0222 >OJECT NO: 06 ^TE RECEIVED: 1 2 -0 2 -8 5 SC: NOVEMBER NELL VATER CHARACTERISTICS CONTRACT LAB: CONTRACT LAB COST: 0 3M E -L HOURS: 2 EST CMPLT DATE: 1 2 -0 9 -8 5 DATE COMPLETED: 1 2 -1 1 -8 5 IMPLE DATE CODE DESCRIPTION TEST RESULT 1 1 1 -2 5 -8 5 WELL 2 11-28-85 DI MATER COD STANDARD PLATE COUNT TOTAL COLIFORM TOT FLUORIDE (D IST ILLED ) TOTAL ALKALINITY (PH 4 .5 ) TOTAL HARDNESS TOTAL RESIDUAL CHLORINE AMMONIA NITROGEN - AS N PH S P E C IF IC CONDUCTANCE TOTAL SOLIDS WATER STANDARD PLATE COUNT TOTAL RESIDUAL CHLORINE PH S P E C IF IC CONDUCTANCE < 0 .4 MG/L 54 COL/NL NEGATIVE < 0 .4 MG/L 220 MG/L 260 MG/L < 0 .2 MG/L < 0 .4 MG/L 8.0 450 UMHCS/CM 290 MG/L 1 CCL/ML < 0 .2 MG/L 6 .7 1 .3 UMBOS/CM * = CONTRACT LAB SUBMITTED BY dated n -n -lJZ 00301S