Document jdqxOd9qpRLMGMgnk4jbQgM2

F e b ru a ry 27, 19f7 H r. '*. ?. 'horiuis toncr.rch D iv isio n b u ild in g I.o. 23 l.a t i c n n i Canh Morris t e r Dayten 9, Ohio X Dear Dr. howac: A ttached in a p h o to sta t o f the o rig in a l paper of Dr. Jensen in Sweden, r e la tin g to p o ly c h lo rin a te d b ip h e n y ls. I w ill bo happy to have your id e a s a f t e r you reac' i t . As f a r a s t!io s e c tio n on to x ic o lo g y i s c o n c e rn e , i t - is tru e th a t eh.loracne and liv e r t roup in. can r e s u lt frri- ?.*ver doser. ' W h eth er o r n o t t h i n i s a t a l l r e l e v a n t to c r .a il o v n u ti tie*? e x i s t i n g in human f a t i s , o f c o u rs e , r.n e n t i r e l y d i f f e r e n t q u e s t i o n . At any r a te , I b e lie v e before.v;c worry about th e t o x i c o l o g i c a l p a r t o f t h e p ro b le m , we s h o u ld c o t t l e tho a n a ly tic a l p a rt. S in cerely , n r.i'/in a tt. R. Frnnot K e lly , i. D. K cdical D irector NEV 0 3 lU ti 780618 l.J... - * (i b ll J .'tlJ . Iw . n n r c h l'i>c X^iu- ^v*i^*irnw>*i.%j.ii:: --JLj./ /j7.*#^** - *-<'^irur\ / l l . Vi' 4>.W4*f*. V..4 1 i ' r , t. II* H . 'i l ; / -C . _vr :c L v r v m **V*.* v,# i .r*--. v :.-L.L*.-C " Oi' v ir- i '.!/C:>XT :c-.l i - ; iv.v h ' c v . j t cvi-:.rttin.ViU2 t o r t u I ;*i^yo n c c x v c d f r o n I C \.:v ;:/*\ v m i l u >c? A i.rv jf* ,;- c d X:\ r c c c i v i n j a C 3 T / o:.` i . ' i n f:w:v::.t; \ j c . .-.m *.:1a v . l s i t ;.it I T a y o r . / . a y e u c e a r e a l;c: r. cv.o t. u ; r j e .v x c n n a ix v iic n a o C i:v .;\::>. ; l c a r ;.v ic h I c a r* ;.itbj.c. ."j/ : .v jti v.i: i y c* . T . i ' W * i ^ o . :i* .v/J.*" *;;;:: c* `a c . I w i > i : i o a : iv o .V/c 1 . . ; X 1. : ........... : t.y .V r;-. ! o / ,a iif ::.r.7 l . i;, r *. : : ... * .; .. *. . . . u is. i u n<!.a4#' %'*a. > .i ^ 0.7 4 %.* , *%*I%*."`...o y . ViiO l '. I#i *144*. 4.. .*' l' %% % ..m> #*%'iIVS 4***4***'..'!. ^ *' ,> . M #.#4 *V4*'*%v *'?.* > ^*%.4r TX* u v c o c ;:& :v i i `.". v a 1*3 -j * * o r j . ^ w r ^ u c r i c - 3 1.1 . j !i wO '.w 4. jl < *-"01 T/-3 U> ~i~IC`UO t /..J 'O* . . . :.x a \ v f o l % j o w i r . v o r:iy c o .:i:.;::n tn o r o n ;:o.cthir.:> i;a lc li >j *. *v. i . *a o 11. * f ^ j.* i#.'** w* w t vi* . X ... vVo h v a i - u .'.j ; A'i v i c v .v cuoV.:>:*..7i,,o o i t m iv o i i c J . ' n i a c c j 4 `j r i.:::v*w. : ; 201 2 ./.U* ;*x I ' i o o . x).. c -ilv 0 . . . i* ...j.i::o o lO t : a z i:..iiv c.y i.;; oto:*../ c v i a o I 'O i.a o i u o i c a l i l ' / ' I n o c r l r ; ^ * V iv jo !: yov. T o * ; / : : u r c y o r s c r o t i o a 5.n lic O -v ir.-j u g X*o 3.1c ; t h i : > u o t i v u ; *.c C'.oi (/V ov.;c`c o a i ' i : o c * i v j f G p r o ^ o r i . ; ; n e n e v;ildc* NV 0 3 l o l 780619 j,.|,,. j1 .1. . / 1n' ' t,V`inMMi'yinN n \ .11. i m in .-n i v i ' iteti:, tu. i:-iim*i; .in a livi. KMl Intvi/rn'ttri' tty f.7i.Wiui/nf n r7tlir^ it:ilurii |trn-l>if|<c |Hv.-ml in 11*** *.V- Il (fitvii/nli* *1/ Irarl tiuy rnmplirnit: tin* ana!>>! *; nr fi'iue |,,f1lrtff i.'/niin*tr *. riiiirr in 1/ in.-aftivnlfi* limy iIii'iiiL'tlly nr rataiylirally ./Iftehn* /M*i/!r*s*:n *Wi. SturlitifUn 'tii'viiniim.'r ilmim; llm :iii:il>>L*t. Fur llivrv rvasiniA, t^iiliicrf of nr^.in*t- ! f.nt--iintnlii,r-*f tuttinmvii Imi ridai im*- vlilttriin* vinitptiiiutU uluiiiM I f riiiifniitol lv .fiiiiiiS rnni|Hiiiri'1' li:ivr Invi ili'lrt'ii'il 11 (tiln-r aiialviival ini'Mituls. Tf i-inm^li malriml li'Diti In fili* nnliinrv |n*>iiri|i'.-<*in fIn* van In* il.ilril, infr.iml nwu|ty nr nta.-s f t'.llllpli*.', ll>!i lutili liti' rlr l llnil liiTlriiivti|>y .ran p iw pitivvrfiil rviilt'iivi* uf ^iirr nini liti1 liii'Tiimiiltiiiv-tiii' ili li-rlitr. itliniiiy. nnvc*vi*r, iilt nlltvr mvllitvli in f... jffr:i n( nnttr f Mu\m* l'rmipuiimU ruiunifiti H.-i* IIn* pliyyira! lu.-i-t mulri lyiii^; .',111'ii ttlli n tfiftllfiltriI jfrtaC rhmm.iiu- Krixirafiuu m very Htuilar. liiOtinMui cl of. |t*ni:t*i| f|t*vfliiiin*li*i n.lCI!-!HKKI) itfili*a (-1 n,ittrti*l a In'sMy nignifivaiil nni- 11l1.1t liutai nf iltt* 11iiliiiiiwn riimputinih pnnlamT lit'tum i l!in ''`ii-valni's'* for ixsli- .% pilvrliluriuniisi liijiliflivj^. l>n|rii(i:iliil riiEuit in IIir v.irimw par It)inn ryft*iiL< , -nll.il in Il.llllli* IntVilliU lilUH1uf IlL'litT ili'M*ri1f i l Uy llrrtnta anil lltminan (it). ;tii< nf vlllitriivilii'ii. A l;irsi* limiti n*r nf 5miil:tily, rvlvittimi limN mi <li(Tcrent Cll.C ;Jiv<Iimvp Inni rxnmiiml. l'nlyvM-.irin.ili il xlatiuiiary pltaM.'t ami /'/ nf pr5liriilvi in h-jiyh all1finititi o jkvjWv in li-li and in. various T l.C nr p.ijN'T rlirintialor.rapliit* 'tliiril.-t, Imi :iImi in nmifrr lex-dli*.* :nnI in x.Viilein.'f are* highly rnrrrlalwl. J'iii'.Iht- *.f/.iinph* nf himi.in ilepnl ini. Tivn limi- 11hiit', It[ valine in ji.tjtcr rliroiuatu^raphy *| Mtnplvft nf ku:1 nini tv:i!i*r mnl livvuiy nr r if e l y rorrrl.iltxl with Rf vnluiy in 5<|i!iy nf (erre.-! lial iriammal-t cnufaiucJ mi Tf/C and will p-wtlurs. It w posfsibN* nt;ili!i* miumiits nf rtil:triviicil liiphtityls, to do a Rival deal of work uilli the in I*.<ir.il fU*5>*vut'tv are giu*n l.v .Tcipni nml tention of cMnhlUIring (lie Urnlily of a V:!iiLiiijM n^ Jfy u.-MiH .1 liif ratititi iiirtlvHl, rotupiiiind, hill very liMIo additional cvidvm r i ni Sliguri lina ry piv-tiridi'* rati In* rv- will lo Rained lieransc the rvsulls from f Ins ini futili rxtr.'ivts nini lini |^1ydi'tiri1tri1t-*f metluiilt li'cd were nnl iinh'lfudcilt. CUA* ;!r-ny! ivi!! In* niiaf>i*.**;i-l. In ll;h- w:i\. ii uiviitimi tiuii*, an ]tj value from T l.C or Uni $u>wn lliat |v>n nf llu* mnjur c!ir*im:iloRiap!iy lr.p. p*valne), a ^`.mlrtl li:tl*>ii\ ! Iim L' irt'i*r!n Ilio /**-i#<f* f-V.l.V..U1I!ll.*.| Vi *r 1 1.^ ....:... Ir1.*..!.!..!...I il* tf fl,/'-l)l)T nini ri,Jt'-l>I>Tt r-.'.'lKC- l>y rhvmiral re.iciitu, .ill! pvvjtioidal teiivily .u{y. Tlii# mTrl.ii> h* f'minl v.iili ?}-!'f aRaiiiit a stisecpiildc <ir";ni.*:n arc cniMidrrrd !'iinn/, tiwvL frerpirtuly iw d fnr liti* qnan- to he imlrj)*:mli'iit ivmmirtvrs givinp nigni.r- 'tfivc rtviilm* iittalyyty n*irlfd in liicra- e:ml evidence of idnnlity. ar, Imi imi wif li QK-l cnlmiins. S. Orpim|>liri<j*!itmis Im rcllditc HriMwc fif Ci n/ifnitttf.nr o f /frulli'-- I' A*. I.\ FIpur (Siirii Hrsrnrrh l.fif., (filurf; /ferirnflnrr Urtrarch O ilin', 'itincruriiriir, KVnl, Un*tmiti) Mulli'lt'liTtinn method* trnnlil JH**m lu Ih* ruttai fnr Un: misdysU nf or:tmirhl>trine **lm*< Utiil-'o of 111? mimiter nf rldnrhi.tled ' aipnn'iitit wliivh enti lm prcn*nl tu any um* Analyitl! The Cuinmi^ion ninrludrd tlmt while Ihi* n*aid:.try nicthrHU were ndcf|ttale for paratliinii ami nialathirm, rlariliralion of the tcrniinal Tvttlm: rituatinn wmiM lie wctconu* in some iuManvft!. However, mote work nti Ihe rh'aiinp iMago for main! hum rr^iduvn in rig?*, milk, and driixl fruit was uidiralrd. .ni;iff. Ilimvvvr, Ihero. are prnlilmi* uf 6. .Dictdoniv* Hc.-Idue AotdyvI> Vri|i;riattun oven vlieir techniques aro fly lit A. /i, (Mil'll Hrdrnrth /.(if.. **4 villi lite rwultilKm ami sensitivity of |l'neif.riirA* .fcnViifinrnf /!r.<rnrt/i O n in*. vvtnm rtiplurc CT.C. Kwr rxample, flic 5lltngbiiurne, ffriti, OipfrimG 'duiimi nf tic GI.C culimm may noi lie Five (liftcrcnt approaches Iiavc been dv- 'itjt ninnali tu jn*iMinli* rnin]mm*uls; r veh>)fd for Hie dvtnminaium nf traces of 'i|nirilU*i privrnt in ll.u Irvl111ir.1l ic'n!l* Jivhl'.irvus: total ihiiiliurus (4, &), coinrim- J NEV 031002 780620 *f nini > IcnniiMlo! rnd tt*. cu:uplrt''<I chum ivlnt^-d atlach.tl Ijyiiif _ .nylal.uiyl/JiXA hiinf.Jy fi.un tIrvi rlh*i\ilU':*Ml'..\` lv which It i hoju'.d for llirtimim:. With tbit he* lias hern nblv. to liUim* the Ky Ilio IliNA fti!*'vIu carry;.' the Lini. i.tiiii:o-;>i.-ti' coudUiurs urcir.-Kiry for terminal ion; nciihMing fio i'm^ ~~ v inwrwraUil. Thvie bo A inrolih'in for rending compie.\ under suitaiilv eonrliliotis with or uilhout the cur.in iorminaiia1; codo (llirco vod-ir.* lAA, UAG, threonine or Mijx.ru.it:ol enrvines kIiuwcA lhal to go c:ul UCIA can todo for cimili ior*mat ioti) and vhwving release il>is m-ve^ury but nol suftieienl lo complete tho k^ iun pepliilo hy mcori*iratig tliiconiuo omi that rvlcamj is not solely le'vndcitl on the lran*fii nixyincs required to move the ribo.ionio from the lluvonvl e.-lon cif '.Till l"as ~m* :iui * 11:0 __lhn ~n(. L . --a .`iTu-r* uticul in. to tho UAG tcii.nnaiinjj codon. Itc'ease does, however, ltc ivpwU the ducow ry of nil enzyme required for dcjiciul upon the prcscnco of a Miprmalant factor. This* iciminrilion. * factor, with a molecular weight of between *10,000 and * The nijny for terminal ion employ* the MXA from 50,000, seiliinriits between 3*5.S and J '5S. J)orau.v; its an amber imilnut of the coat protein gen*.* of the DXA rokwing activity is not affected by nXn:;e, it cannot ii bnclenuphairv. /.'17. This nui'niit !m* an am her carlo, bo an KN.Vprotein complex. Apparently the subslratu UAG, At (he position of the ai.\lh amino-tukl, a hita- of the rclcaso enzyme is thu l iliosoinc-wiliXA-pepiIdyt mine rci'kliia, in the /17 coal protein molecule. In a fllXA complex so Lliat it- must net directly a t tho ribo roll five system the m utant 11XA diroe1.1 the synthesis some and not cleave tho fUXA from peplidyl./ltXA of n email X-terminal jH-ptirto of tho coat protein with already released into tho supernatant, which was tlio tlio sequence N-for;nyi::iC'%*ala*ser*flftpa-nho*lhr; tho other pct.MbiH:y. next amine-acid in tho coat protein is "iiilr.mir.c, but Cnpccchi does not yet know whether the release with tho imiUmt JIXA tho amber codon causes pro* enzyme works alone or in conjunction wi1h other factors, !j;t>ly ZU in .roc -.ton .wined ol/ttliii jiioa ..,,-imtl ; -rale* : -thouI lu i mature Icriuiuntion of synthesis anA the re lease of tho hoxnpcptidc. The great virluo of this ay.ficm is thnt tlio untcniiinatcA pcpiklestill attached to the pepiidyl. IHXA ran be distinguished from tho lommM cd peptido released from tho iliXA mid tho amounts of .each measured. Furthermore, starving the system for any ov.o of tlio si:; nmiwfAchU in 1I10 popcido arrests tho truiw- letio'n Inachincry before it reaches thb UAG codon so termination can bo controlled. U.vnloilir." this procedure, Capocchi isolated a complex of ribosome, tuF.XA and pctr.pcpiido in particular a hypothetical chain termination fHXA which reads tho termination codon but does not carry an amino-ac'd. Many people, including Capoechl, have searched in vain for such a fDXA species, in d although Kegativo results can never cxcludo tho possibility that such ti'XA exists it is reasonable to think 'about alternative models in which tho terminating codons are read either by the ribosome or tho release enzyme or in which, nonsense codons function simply because they cannot bo read a t all. Further characterization of the release enzyme could well dccido tho Imho. il a -*0. y ;v i*r J Tlio. vy few au or* rJ*. by ictween . 5 *\\ * Chlorinated Hydrocarbons in British Wildlife y X.M l*v 1 fimi r .;,.n lio . M| IIK L 100 j:il*:i<* r i-riivUy L ^h;it:*in* '-- 1 flV."- y D. C, HOLXicS ' 1 J. H. SIMMONS 1i J. O 'G. TATTO N 1l 1 Utamory of the Covcrr.ment Chamlic. tendon Polychtoroblphenyl compounds have been detected In British wildlife. In birds' livers tad eggs they arc often In greater quantities than orgar.ochiorine pesticide residues. Polychloro- biphenyls arc known to be toxic, and their detection in wildlife raises the question of the edverso effect they may have. lily of r -3ffu-.riy : do;pili*. -----Sii. 1 .11 1 Wimx wild birds* c " ; i or tho livor* of 3ri:i*h widfo aro cxamlned by ga**hquld cUromntngraphy (GLC) u^iug l rlivlroii'Ciipiire doirciioii, p-.wkfl CArivp*>:idns to aneti ( rjiinoolilnrluo pv:c(lj 0* DHC, di'Mi iu am i P D T . aucI i V Uicir n^tiilv>ittfi end bro-.ikclown provl t^i, appetir oa tlirt reuikanti chroiiw.ogr.uni. Tne.c p.'/.!:* *.om.nn\.e chloritio in nctitrc, u h li a aubxtnntal eblortno contont, nnd coii^oquenlly poMOss clcciroii-eiipiuriiig prop-irtcn. On filtcono or 'Apiezon' GLC eolumii?, ilio erica of poni: far filoso cnir.pouud* noi-mally bogin* al (ho pomi et whieh pp*-TD! mul |>p'*l>DT are emerging from ilio colur.m* orni cxoud*. in tcir. of rctomion co frur '-- 1 nmoimr* whioh aro gnifioant from a loxivolo* or fivo timo* tbas of p;A PD T . whcb il tho Inai of tho gte.il p>j(i of viow , bu elio #.en-*tiWtv of jr.ot'iu i.iuru* commonly ooourrir.g p:>*ictii.\ te cnwrgo. .. ; . . ^ iwr.t alno enablc* com pirtely infigiiiAcant r.moiuiTv io b*? 7" ";*v .y 'I.C. I./.v** nc.'iailly i r. ilio [ _f.;nn ilio *"*>tnii;o* 1 1*X \.v I1 *t <)'.'<:cicd. Att.ilyjs cntiecrucd wrh il:*' (lviiTir.imuion of orMr.ichlnrr,i; p-vnioclvs in wiUkifo, .-v.wvv;-, li,ivo loti" ben *w<uo ib.s-.t it r.ot umilimi ibr m\ amliior.al sorio u ni lar.r.y a;i ton pooks, c>i:rorpe:idi:t:: ;o wni.l.-ttit;':.:.! c,j;;;hi*;.-J4( i:;.,..-*- vr ><' eiroi*.>i:*>e*r.ii.lH1*1. 1* . ... '* "V.! h. :* *,: 1. *' l __ 0 .' pi.`p ; .1 ir.t!:r.M*.vn e - . * : : a r o r--::: '' r-:- iti p r - '.*.1in*. j*..--;.g*.*.? F-.*.':ui.^o tbov au.I ;*rop-;rii.M Uv<- i.-. l/,,j /tr .i .;* C-.---0 e^ui- t> l;;*j p.-*lioifu*a, they t.Im .-j liitoitgil ".i,. fiKin-!,, l.i:, j'.cp'v u rn 1 e>tr./i.-'n.'w ;'d i: ti:**y oro or^.vn'i- 1i:*'.imi sitiiiuir !. vu-uji pruovJurv*; cut.i.'4u .\ ly , thoy NV 0 3 1 0 0 3 780621 7.20 cf. cn' tcri cml y v:!l!i d:*.r:K'.in".io:r; Ol usui ;7'0>i*yX' i:i Ivreau*a ;.f ovoi'fijiiin" oE ihe chrOHialo'it.iphic pi\d:*. i'tococl"rcj hnvc Ikoii ti*v!cU to mvrc-.v.v*' li* ' i!:il:cliy of ii!c:i;n!i;; tcaku. One nviii'Ht i> to cftbst. ri be',ter rtvelutio of llierc p.iriietdxr pi\xfcs by citrini cf n nxtttabfu tlRlionarj* pli.>*o"for OLO*. Anuihor mothod. w]it\*)i fl!?o d*'r:i*.*;:str,Ucfi IIto prvioiico of Itieio coic.i-ytimU in lito ar.ude, il thv ujo of n pielhnitiiiry thiti-Iiiy*.:- cin-uuinioga-phic treatment'* TJiO clcait'.'tM.'j) cxlmet* vru OMiinticd o:t ilicn E>1 cliroNi' tm'lulrs uslng n 1 jht C'.-nl B'ilution oT rcvlMn* in hcxtme tw mobile plnse*. Spols rnrrv.'pon'.linj to th compound* cali liuti bo burn oi A> valutu botucen 0*$ mal 1*0, 'vJvro.M ilio co:n* iiionly rctfuri iifT oriMiiotihloriM*. peilieidi* and tlicir iiiet.-vbo'.tcK produce po'.* of louvr ]{y vnluos. A fimiinr epura: ie cari nlo bo thou*n xrilh vmto fjlm.'O p.-.per chtor.uUo- propliy* xvhercby th imki.ow compound movo only n \vry liort diV.r.veo trni tuo Inno Uno romp.v.cd xvitli ilio pciticitlej and lli-ip me!abolum.,.. . .... .. ......... y thw-Iitjui'l curomalori iip'.iy dur.l* elitimiei detee*ioti y*:mi v.th oparc.to electron-captare nud ilnine.io:r>v.en rictco- tuM ho cv!<> hoc ured to donvonslrnio tho proionco of li'i^o unicum of w'.?uk!y olcotron- Ctp'.Virinj; tua'.. rial, in nxUti'.!p:\ ta kuwn pp*:iciclo t\Mduci. in tho of oyntcr CAicher* {/(fff.c.'o/Jtri r .^-^;.Vyl/;,. Ivxporioiitm in ihi* inbojv.'. O!y lAS fllOV.'tl IV.ni ll-.i-ro compound* (.nc*:r so*t fftcuea'.y P omi In ibo h'ir-i.!- yropori'im-. ir* ih? live**, fui onci eit c-r biidr-, {..je'jli'rly icrrTjlriot NATUKE. VCL. 2 t0 . OCTOEER 2 1 . 1SG7 'r.-dr.t^TT r.'i1* s* **..-- f*irr/Ti*.v niViwJ (listi kc.itvfa (/u.Vu (;*icv-Vr) r.v.J co 49 . SO inerirci frodet* t-ueh oh guil'.einois {Uriti Retention tibie (ul:i) P ro.'yvJ ni:! );;`l!itvi*l;cj tr:h e ty h )t tlv'y nlr*o conuionly ccour in frt'ihwacr j'iff. I. filiti. Wo lmvo dotcotcd savi!! fimounia Oat'lIq'Jt' ebro.tatpjrp!.' pi (*:>extraet r>?kciircl IItci uni (5) ntraticM * pol>chtJvb*?:ii|lfo#!. | in lmr.vm nud rninv.t fot k.M.'.jvIoi, bui Io frpciueaily u:ul in murt *mnl!-,r ]>rO|>oi't!on.i projjortics. Tn the prcrrIbcd'cxtructIon And GI.C condi tlma in tviuu x.imidc. tion, the.io mixture.* produce c serte of cliroutAtographie iA rimilnr icrio of chromr.'.o^rr.phie neaks lm beoti peak of Vise t>pe in quvtion. ol'jcrvcd by Jtokion1**11who:i oxnintiitnj c?:*.rr.ct of ca!s Tho compoui:'.1. occurring in Britleh wildlife corroipond ftncl n<h tnlit-n ta Soottib vi'iiaw . >Io round ihnt thoprck to tho tnoro hig'.ay cEdetr.ia'.cd bipSiouyl compound rntl::r given bv th? extraots of fiv.4i u:id Afn-wiier iUh r-'iircsm. tbe.n tiro more lightly chlorinated type. Tltcio IiigMy ted filit i)- low concoutrntt^iii, bui tho-o givcabycxtnet chlorinated biulieny! r.wo occur to semo extent iti the anaiogou* po'.yeidosoicrphuiyl ptunitr.ilions xvlrica avc compoututs. littlo iirogrcf.t Itn been irutdo in identifying conutwreinl set shniuir to thoo o f p*!ych!orob;n!i.'nyl. thorn, b u t thoro !nt<t long been a goacral vupnoii'.ion th r.t It in pob'iblo that tome o f Lite compound v.itlt very long thuy v.ero eith er fu rth e r brejktlouTi o r com loaffttion retention time ec:*.trHiip in bvitbli w-ihUlfo m*o lightly aroduct.i of tun orgenochiorir.o jw p tie ld or p o iid y elderinnten polyv-iderotcrphcnyl cotiipouiid*. Ioo%9 comp /u.'.di of ilm tt tvith tn itu n J pro-IimU ru eh ud l*ig. Iti hour* the g*>-lini:id clnouvitugriun cf mi Itioti-i:*. n..:, tC'r. , extrnct* from rv heitrrl liver. The jv.ihi corrcipondin^ to . J.n -eri. ifowavor, uen'cilvd tit idw s.!fyiig n erio* o r '*X)J>i) (0*-l3 :ig). Initn-V-lIC (0-33 ng) n:;u c.uinn.v-HHC uicti jkmU ii-. f.nrrc.'iiiiiidiii^ to tw>Iyc,'I.Ti.ilfl,i,ny! cr.i- ttg) lire I;'.l'i,.!i'd. Tlic other pivh-* form the ir r."'i t.ih n i in clihiuciit pari* el' wotlon. tvfvrrcii to ti*m* ami until now w-euM h a w bwu t**g.m!*Hl And in in. . V.V ii.iv*.' no*.* tivva iibti* to a!;\v itir.t Il!3ii<*t I*'U!*..j: tfv*:.-i.'.,,,.*i*!:it ltin:. rc**i!:-aiy,'i*.'i'i*!i.*!t-si cce'auirrrrii:a^ ilnl I 'lllb ii witJ'f'i in.* ;;,*> ] nlyi:!T.-j ;:i`.*:i>`1 c-itupviimi^* l'i.'yi!*! *i*.{.;V1*;j*i:-::iyl ti.*< l:tv:.*:ai in w.**>::s w fat <CO:nr:''r- ci-il u*o f.*r r.i- r.-- a ic-irivi?/;: in p-iu.t.*. re* Will jit.. J; , !,i. *>! I i*c*:* do/* ftt.*l rirtiply ft* "unhunwii c-rg-iimciilnritic ctmipounu^ net c*iri".'{^nflin;; to any known i*vticide of ix* metaboTiteii". l*ig. lb flmxvA lho iUiixlegotirt chronintegntax of n CvVivnor* oial j elye1deruVi*i r*.-d:i. So f.xr p.a rc!v:t* >: time are cotiy'tnl ia*l cp.sit f:o::x the p-.dichl* jKV.h/. th- M i-i iixnnl i:i-.!t*!i:t:e nil hut u fo.v po;i!w in th*' two t-'.-i i : : r i.*:i : 7 . '>' :: *1:-* (m .1 i. ji ** i r . r * * ii.'Ur'.'.y P -'- 111' l!*;; - r.J*t in:*, but nil **f ih '.ia ..i.:. i.ly in r.'.'nt--*.*f . o-. lt'.J':i"i'y'. r -../ *t.v (*"!*; !* i*vp*c*''d I" ''MV.'i!'? I h " i ; / -ill n'.!.-*** lri.-Lcii',H!:,..l ;.d phy.'.i*.*.i Ti:c clir*>:r..L-.,'.r..v:V' rhevn were cbt;*.tuo*l by x:*>e cf ft f ;*>/*,(* (<*-.V-ii/-; .7ft cehifxn, hi:*. tii:< nrcoi*' enro* i,;*f`*Ie,i*w h.'vv.*-*--: :he r:-ii:Ion li.i.*cf iho j*/'*.:*for lii*` mtbuOv.'M C!>::*.;'***:i:iN r.ii*,:. t:">*o 'r*':x the jmlyY.or' lili'hi-nyi ii'.dn h ; * . b * * c : . '.:**:r.m:**N:;-'u na /.* NAT1*; ive.'i i. yu: 1*45 li'v.Ji T*-'->x; Vtzii xu; Oliti ex rorcrdi given i Furl pa*.nf! on th obiorj gi'txnix. lo ri cheat titan!*, iiifts Instati otdrtn Ai Beili* In tit* for tie lion f myvtc fcowo1 the*-* end i lifo *t very t s C' nuxrh to hn P. F. Untve Sclent It i ltJR'l anxU both fob c <quiv Ore ii to be tipC! Ml i-1 CC. XIU'IU lat/- let I fc::u 1 1; * t'.l--" Lil'**: 780622 NEV Q'lOth lkl/lo 1. -. , *m s .il*' : o;` (ilv tu-:II.. .!.<(.* VI- * .1 (li. t *. ) tHi1' ' <t.v- M tv'.iui.r. XL!'. Kl .ir..-'" t.` C-ihll*. n i ; rei <*. (/.' .Vi J k u : i'Cll !Via 1-fl tat*-.i 94-1C15 tii 1 JO M2 1-7. 1!>0 32-M11 J 70 4V.S t i MI r :i re e-67 VC7 3t-0o1; 1ir.*''*. s-'t: e et OSI C-.'.il 1-21 l-:;l 1l*"21. i t-IU -2-*r.r7. S-f.il t-ac s-;*> a i v'Go lu i > *-l KMJ, Kfitiol Mv**rviraci; rCJ', j-!>ca>:wM7!KT.>l iffin. noci cynno.ulicon*: co!mnr.n, TiiOsiclr.nl icli'it!u:> tiravi rocorsoci for ilio jkui oh t!w>.v.- thrvo typ.'.s of volumi! oro gVOII !' 'lilbt-J 1. ,* Furila'- coati m r.lion as (o ihc id o id ity o f tk rso com pound! bus booti obtuincd by a idudy of thvir hohnviour om tliiii liiyor e'.ieiiMlonlnti.M, /dam ino noci .dica gol r.biorptioi; cn!ur-.uis od ruvor.'o ph.no p ep a r chroiar.to- grum i. In nddition, thco e i:n;o;i:wU hnvo Iv en showii to rcfO-.nob t!:o polychlorobiptui.yl compoi:.de in their cltcmicr.l inori noi a. Thi< tlioy uro r o t cafti'.y m odif.ed l*y eitnnlo cb-n*.ivd rvr.ctiom to produco rer.cily ii!o:uif:ni>!o ibifi in ili'.-ir rvicntiori limo. on t*J.C ns c-\t; bo dono, for iiudmicc, to D D T by hydralysir.j; it to ;*//D D E or to iddrirt by oxidi/.ictg < tJ dioicirii. A nutnber of livori nr.rt oggi i.ilccn from b ird s in tho licitivi I*:!oi or thoir cornuti v.vt-en h.ivo beoa oxaniiaed in ibi luboruto-y w iib resulti fi.r.ilur to tkoso obluined for thoUojiltol iivor roferrod m cnrlior. W'hilo (he ideai .r.e.t* tion of vh.\o eom pouiuli in D ritiaa wild 1ifc miy coivo tao m ystery of tbeio peak or. CJ.C caranir.togrm n*, it rr.iici, howovor, nntiiy ovh^r quo.:io:;. W h y , tor o:f.nr.p!o, do thojo compound* reni to bo oocu:r.uir>;cd, nv>.t frociw m ly end In tho lur^rvit pmporti.y.iv, in eoriran typo* o f wild, tifo iup.Ii r i bini., evale and fu il, an d o nly r.t tl.o rno.-t in vory invili p.-jportion* in nv:n or h ii comoiia anim ala meli r.t er.vc or sheap ! Ir. thii* rcspc.it lltcy sw in *.o iffer rnarl.vdly fremi th oi;.r.oe!U'.;ir.o pesticide.'? w l.lch coc-nt lo lir.vo li*va Irti r.cv.ily all ^oc:* of o 'ir onvlroruriont and to V.baili ' liu ir a itro n j( rct tn ib lm ico c l a m a \ y ; ,( (liy.iically. Moir.o iif Hi i nmpl- iM /rd ; oxmm/ hd in l hi-; I dv-, )i.n\i u v m c d l c<*at;in h 'g li'r pi*p;;ion*i o f | e.';. n-i. biplirnyl c im ;iji:i.:L tin i: or.;.m y:li!'.rii o j*t*-.`.r:*tl* .. r c r O.Wis.ipS, tl.u |. ; i'm o a th o *:li:oav*t-;;r..i.i for ib o i.c ir,il lu v r kIiOwii in Ki,;. I w rro c d in v ilc U to Ixi c i|u i.n i.r .t to n lm u l 12 u-; n f |r, *lycl.!ofil:;b n yl e om p oorvl . 'l'Ii-i lU'v.i ho c o n ti. ! d ii".*iiivt ilio r-rg.inoclilntii.* -in*il r.siiiici liste*! i-l-ovo. Titi'M' lo t.il o n ly i.boa:. (>:'; >f wliicb 0-3U r.rj is l.v ta -liJLC, on isouv.-r o f leiO wliicli k gonc.illy rc;;.ird- il ns non-toNic to w ild lfo. Crv.it C`nc ra l.os of:a:i 1h *,ii o\pr-ss^J b y coim i va*o:- i*i* ul/OuL ino cf:V,ria of poliicidvs oa wildlfo, pari e dr.rly b ird i. Ti.o dvafifiVAtioM of tboso olbur o:".ino:iln:ino compound* in wildlfo proni*:*: cncjuirics ns lo tbo p'.-ublo Ionio oficolc tln-y in.vv bo liaving. T r.ut thc.so coinjiounile ero toxic i* poner.dly ngrco(l,,iU j Ilio JKnjvii'orobi- plumyls oro la f.:ct ro^urrlod <i* nn inilurtrinl l:nr id r-nd tlu e sbolli lim i'. for in e in in nir J.r.vo b-cn ad v i ' / . " . W o thmil: tbo N aturo C on^ia-nnsy for inir.plcs oi v.iidiifu nr.d M o.-ivio Cborr.ic/d* L td . l'o r ol polycMotobtpli.'nyl rosine. lU.VlvcU Qiir.yi s, j>;/. r.ot.'-.rn. /!k-?*!. CO. i '.l W X ). *l!.vrl*or.,l:. li.. J. S'i. V**-! Azrit., 17.10(1*1-%). */>.'/!/ ite C*>-ttfU'tn'. CArr.;, 70 (KM lilllor.rj y OCira, ).'ia'!oii, 15C) Wr.i'{,r. C. H.. I!:..-.ilUorv. C. A.. *nl IlarTlioa, It. Jt., J. S<1. i e:J tjrle. XS. 123 (I3ii7). Jt Viaitcrt Mxnr.lor. M. J . . i's-m. 11.. Coll}-. K. V ,, Usir.iO'iaJ. L Vf. Koturii, J.. ai.l i.';:r.<:i,J., Am/yW. 1?. W-j (IV I). ' Jlm i.ont. ). H.. .I Victor.. J, O'G.. J. CAest.'*..27. SUI (H-77). *AlJiiti, H. )!*>*. lt..an<lTh3i*v.i, J., J. Clrt.*Ac(v7.,lS, 4SI (10*71). *r.xan*. I'.. Ant'jii, J7. IO (I-C2). Avi-.-/ / U.4 Ctn-MtHdl CAtmil, t32 (UM CUtloocry OCice. I.or*ioo *li'iM-'ti. X. sud Vari*:*. K.. J. 'r^t. /p.f. fin?. Puri/., 2Pi(ICiCt). " \. V., J. Al :*. />*', J.V .I. I.. (3 (JtW). *' . - i e: " Vi. V. Ir.;:.*. . .'.* * 7VJ.-*-./:-* / /. h**!.l ivW.i.::.*- C*>ai:a:;on,Kiw v*i.*k. It&l). 197 (RJa **V.Wii, il, 71.,C..- S3, 73 ,*107). '* /le*.- t-Vt'j.'Cs* */ T'*,-.Av'J /.**.** l'i.'"*!, 17 (Coiull`.r ftn t i K'In'li I.iuiiv V..;.#. .vi. Quasi Sl ^^!i-ar Scuro SuDsrriovai ano Novase by P. F. Bf^OV/NS Uf.l'tcrilty r,f Msr.clics:cr Inult Jtc of Science it\ Technology By considering the opsclty of i lurfacc layer of &star, the r.ushoi concludes thee c-tusi-stellar sources, superr.o/ee and novae may b< different manlfvstations of the same mechanism. I t it m u illy coiuidcrcd th a t Iho c u tb u n is of r.ov.io and ujKmiovno nro fund.'..menta lly difTc.vut proccisv*. but* I hall hero propose a r.ioclum im wiiich can account for both piionoinr-:A, nnd vriiicb a t tho K-.uno tim e can account for other problem * concerned w ith q::*'.ii-4t*.li.ir ottrec* (quiiiarn). In particv.lnr, I *'if:::vst tlia t tl:o?o o u :b ta :s fcro lilu ly to bo OMMoernpio {.w indoo.l ib o y r.ro o '>~.irvcJ to bo for nevue). |t*c.ui*o tno oaorcty rvlsV. ?rd d u rin g a f-v.jiornova outbiir#* i* ooir.pr.rAhlo w ith th e itc lln r teat energy, r.; r.ni/atropio cu tb u r* : could kv.ai. th a t th e r ta r i* ncvelvr.v.od to ivlv.tivistic vd.-.city in order :o co::<*'rvo momeultiM. i a u r. a:fo:dl:isr n i':*pplor ox pie;n atio n o; tbo largo ro d -d ia ls of ;h c c'ii;.-si-**.-'Il.:r i<o;irev.7. Con.ddtr ;!.*-* opacity c f l a y e r , /*. r.t th e <;*rf..vo of n edar. L*'t A/.' hr- t i'-t tliic\-:>\-. an d ! ' t l v r.i.i:;:* of /*. oral h i A cu.;i..in r/.a.f;:..i 1.1 ;*':r..;..:.uurc, T. will elcrtson nn'l p o d tlv c i*.-i i'i-it* i-.- .V. ..:.d , \ \ r.:>pv.;iv, ly. !. will 1*.' opari.. : r, -.i of vcl* / <*-v.v. : vt ;i. ~ :.i r l***u; I, .-a i i.. o p - i v d :#;!; of h. A ju n j.v van 11*>Ii..r\ i t i., - , f , t tb o o p t;*.*.! i.it-.hn- f.s !:' 1. fiw f.v * obsorpticas. I t is know n tlio t *r//ccX'2'*,,,.V#.V<A/.' (ref. 1) wioro induce. em Usions r.ro ir.oluded. Sitpposo, i ow. tl.i'.i L is con:prc*std or dilatori w itb o u t elionring tb.* tota MimbcTA o f io;;*; tb .it is, A7J is vrnied und 'r cor.l::ioii; w iirh inaininin c o u ita n t (rrU-'iMi.V,, to tb a t L.'X, Constant. E v id e n ti/ - // rf. A'r.V(A1%v. .V oc J / Al. T hu coniprciitou of L incrcasos il* opneity, whilc dilalioi rVvIncc. t!;o op.-rity. O f c.uirsv, tM Infoi nv.mber o f ions will noi ronvai: couiin:: in piv.vtei*, bccaus) tbo rvco;nbinaii>:i rate i ori: Nili Iona! co .Vr.Vj. I f tho pcrcor.ta-jo o f at.m .s iunirv! is largo, tilt* clccisky o f uniorir.vd ator.*, .V. svili ttiOrcfniv bo premor io;*.'.', to iV,.V|. 'J'his in can ilia i ;l-.c contrib*tic; :; tb.v r.p.ioby n u d a by bour.d*baui.d en d '*;:;.d-fre.' :i.;;i*iti./is v i:Ilin ur.ioni.'.cd moni* is olo pr<*:\*:,.i*':.il ia .V iV ii.'.'. T:i effeet. rr? o p ii* c J lo t a ra p o ;: r t i\*: s : p u l - a c l . i n * * * , fu rth v r i::cn.u-ci tbo o p a c a y e f L on Cx'::.pr'.i**;on. S^iro ;.i;.-:,,.`1u-. :.sCa o t h '.i v.trvib!** furlV*? c*p*:/ *.i'. ni.v he v x . ; : . . U n d e r cqv.ilibrium centi:*V.* ila ilt5 J 780623 I r .t xvcK-'bc*, 'j 7 ,. , A. iohc-vdEOil, Mvl: v\ ;hl :*A1 3-.'; ero. le IV, I.iw uifijoou^.o, ix r.t* .*fj.j.:' M r. ilic h r a \i* ; i, A vnch V.;- Ivo Miic; ;-u*i iur.od i.e co:coir,in,-; tho u tili;le c ilo rin ft- conLfcnin,- rt-:.iiuoti fout.d lu v lld l i f e , or.d you rrlc c to t you liil Lo liih liS . iu I l.iti -y ( o r uuu;; ;j 11i t i l i un t i ty ) vi t o ono o r o c o r c f th o ...color a : . -.lo s v aici. 3 ;.:-d :*.yJe r.v.'flIv.Me lo you. You 'JLvi'orLOoV: to l e t m :_vu lo . i ; i l l a , i>i*L co fu i' ihetro uv/u n o t cor-c to ;:::Vo . i- ovi i im i c u l i o: ' w i I t a Cj>v-.'nr e n t lr.v.*07'i.ito r y r` * - y # . : PV-r-';!0 ) v . i c c l - i : ; a tiu * .t *v. r, i : x 0:11 : r_- i;i;i:.-U .x*. I i l J ;* ifi At i ct-1 : .* A if 1 o.*: < I r.rulfiW.y . 'J i i r i J / i . r ) 1 vouH uc Vu :.no;/ hoc fia* y o u r oluiOxvaUr-nc c o if im th e vori' o ti.o ovens eut i.VtiOirjilOjLV u;iJ to v iu t o / t c n t T i ; ` j.y, you d i r e c t 'd . - . Yousv. a in c e rc ly , i..-: NEV 03I0 780624 Lnr*iN.ii)Oi"a 1ELEK OCIH AR/SB T U M.'j T A L L L A D O R A T O F. Y . BRO AD O AK ROAD. S IT T IN G D O U R M E KEN T. 2nd N o ve m b e r, 1967- Or. V.N. H a rd y , Monsanto Chemicals Ltd., Monsanto House, 10-18 V ic to ria Street, London, S.V/.1. Dear Dr. Hardy, Thank you fo r your le t t e r o f 1st November, 19&7- 1 a p o lo g is e fo r not having answered your le tte r sooner. A s you know, we have been f o r some time exam ining e x t r a c t s o f m arine b ir d s and t h e ir eggs in co n n e ctio n w ith the p o s s ib le o ccu rre n c e o f P o ly .ch lo rin a te d B ip h e n y ls. In one p a r t ic u la r experim ent wo have extra cte d a p p ro xim a te ly 100 e gg s o f the Shag (P hr-lacro corax c r i s t o t e l i s ) and examined the r e s u l t in g e x tra c t follow ing liq u id -so l id chromatography on S ilic a Gel, by g a s -liq u id chromatog/aphy-msss a n a ly s is and by moss spe ctroscop y, Mess spectroscopy o f k f r a c t io n from the S i l i c a Gel column snowed the presence o f a compound of nol<*cnt*r vx-ight *i26 v;hich co n tain e d 8 ch lo i Inc. oi.vn.ti>, Litis i s c o n s is t e n t w ith an oct& ckluro biphenyl; g a s -liq u id chromatography-mass a n a ly s is in d ica te d the presence o f compounds o f mass 324, 353, 392 and **2o r e s p e c t iv e ly . These masses are c o n siste n t w ith pentachloro, hcxachloro, hcp ta ch lo ro end octachloro biphenyls by being present. In a d d it io n we have fed c h ic k e n s fo r two months on a d ie t c o n ta in in g 100 ppm A ro c h lo r 125*1 a f t e r w hich v.-e placed them on normal d ie t and c o lle c t e d thu eggs fo r a fu r th e r three months. E x t ra c t s o f these e g g s have been examined by g a s - liq u id chrom atography and in d ic a te that a s u b s t a n t ia l amount o f A ro c h lo r 125*1 I s e xcreted in to the egg, the maximum c o n c e n tra tio n b e ing 9 ppm. Exam ination o f an egg taken three months a f t e r fe e d in g was d is c o n tinued shows that these m a te ria ls arc very p e rsiste n t and that the le ss c h lo rin a to d c o n s titu e n ts are more r e a d ily m etabolised. I would soy that our r e s u lt s are In brood agreement w ith that of the Government L a b o ra to ry and that we have no disagreem ent w ith them; however, 1 believe that they nay have o v e r-stre sse d the lik e ly to x ic e ffe c ts of these m a te ria ls but a s you know th e re is o n ly ve ry lim ite d e vid e n ce fo r us to go on. Wc ere c o n tin u in g our s t u d ie s on the c h lo rin a te d bip hen yl and w i l l le t you have Further inFormat ion as soon as it is a v a ila b le . Yours sin c e re ly NEV 03 L0 8 7 780625 Vracor.'i <! Multiplet flection f.rttix r/ir.bol 10 10*' 1U-* 10* JU- 10*11 jO-n lu-ll tl-ci t'ftili litilli iiu'vro UltilO (''O Ivl'UO ulto d V I!1 r n P1 a multiple prefix 10 di.lin lu hi'i'to to' ko 10* inc|;s lu* C`!'* H'11 tem To Oc fwtkKfl u nceh w joi-lMr. ^ symbol da* h* k M G T Coiu.p'j'.uid prefixes nhouM noi Vo used- Tiw* 10-* mv*.ro h repre.*cit!*.n by 1 inn, inn 1 111pm. The uUuching of ft p tth x lo n unit la e.Trct fc-ii-.ittulcs ft now unit, ro that 1 km l (l:m) - lOV.i not 1 l:(m*) * l'm*. Where ju'-'iiblo any nmnc-i ioul prefix should ftppcnr In tho numerator of nn expression. fhjalc.\l qiMhlllf le n g th oro volitino mtun donaity forco prcuuro Itemi-,].'. o \ioM i l*'HHry to Kl, wtlll Iheir liliuivillcllls Unit un^V.l&nt inch foi*t ynrd Itijlo imuiicM inilo qiniro inch >W|iiro fovl squnro yiml ijitiiru tnilo cubic turli Cllliio fl-Ol l.MC. r. illon pound lug poundfeubio Inch puuutl/cubto fool dytio poundnl tk>uml-foreo lilogrmninu-forco btmojiiioro torr pound (fJ/eq.in. equivalent U-** m U454 m 0- 3'JtS in UI M :n (curt at km 1-sr-3 iskio C-fA*1Gmm' OO'Ji M3 m* O 0 127 m* 2.&SI 00 km ) >G3S 71 x 10*1m* 0-OCSf 3K. &in' 0 001 64ti UK in* 0-153 5U 37 kg 14>303 9 5-707 00x10* kg nr* 10 0185 I:c is*1 10-* X 013S-253N' 4-MS 2-' X D-S&5 05 X lO l-SiSK m * 133-S22 X ro-1 6804-70 X nr* reanochlcr ine Pesticides in Seals 0 ano1 Porpoi ses A. V. (OLDEN K. MAUDEN rlit * IMtt.M, rt*,!|!#.tI#Vi i . i Pul f.ry, fuil-ihfre Seals and porpoises tn Scotland and Canada, far from the sltei of application of pesticides, can accumulate hi^h concentrations* of residues In their blubber. These chemicals arc spreading through the Ion? food chain which ends with seals and porpoises and obvious:}' cannot be confined to their place of discharge. Tin: pr* 'rit<-o of orgrmorMorino residues in marine life in harp Bents [Vhota procrfmob'ci) on the C'cmndi.in Atlantio mnny r.ivm of tho world, including tho Antarctic, litui been const Imvo n!so been oxotmned nnd tho resids c*f there repealed rv'cnlly1-*. In lhitish vnlcwi the necuimihitiun analyses uro roi>yrted liera. Exmuinntion of samples of of iviidim in tin* eggs of ten bird*1 nml thu process of sodl'blublxT from other aross is cuntimung in order to c o n c e r t : ( < f pesticides through ccrtnin food c-lmina aa<M*s tho vuriutloii between different regions of tho marine In tho Mi.iino environment* Imvo been studied. Analyses environment. . of vrri itH tpct.ii'.t of murimi fts.lt and nvitmmUs from All lU-mo snmplcs from Srottish waters were frozen &colti.i!i water* during the past fi yerru'** huvo shown at --1$* 0 until required for tiiKilysin. Tho C'umulinn that or:;onothloririo residua ero now n uorimil occur* samples were preferred in 10 j>cr cent formalin, which rmco, tho hlplii:.-;! (oiic.jntrniiui.a being found in organs showed no evidence of any influena on Ota nunlytic.il Ior iLiiii-* with iho highest Jijiid content. Thus tho moscio iroccdure, and in this and other ceses involving listi it timiic of Mlinoniii*, the livers of cud and Iho blubber of uu nos affected tlio vfUcicncy of recover)' of pesticide ceni* uri in Klor.iyo riic.i for thejo residues. rosiduos. Aliquots of tho samples (0 g) were ground to a Thu i q-.i.vtio muinniiiU, sudi as ulule, seals mid por* powder with anhydrous crystallino sndium sulpluito (AU poi'/, n, have u relatively high proportion of tho Lo:ly weight grado) anti oxlnietvcl in a Soxhlut nnparntiu with re* m tt.o form of hiiltculimuuitit Lit (blubber). Souls feed distillai AR gnido ii-hcxnno (previously tested and found chii-lly on ;iduids nod sn'nwnidH, nml purposes chiefly on to bn fivo of eU-rirtin-ciiptiinng mntrrinh by p n chromu- smiill mid cliijnruiil.-,. Thu prowsj o f concentration tograjdiy), Tho hcNnne extm lu were mudo up Lo ICOml. of th-ir fowl claim roiuhl bo cxucclud Diut 55 ud. uliqiiots were subjected to ctcnu.up by tori -ni: in 1;ij?1*j*\`iliciilvlewbi in ih*fot <fIhrso imutuimi/, ilio hoxnito-dimc'.hylfoTtuamici* partition method of do nml t.i*Mpl".i oiiidyjiud ut lids hiborfdory confirm lids l-uubcrt Mtmiuler if i:L', folfeuvd by colimut clvnn-up oxpi*i!*:iinti. }u recent yenin, specimens of Iho grey seni usimi iituiuhiii conluining 5 j'vr cent water. Thu lo-s of (//ii.i.-.V.iM/ W l'in). coumiMi noni (/'/ion I'iliJimi) nml peslu-tdu riditiiKU from fnt uxtniels so treated is up to p u p d ' ( / `fi'-:ino uti) hnvo br-cn s-uuplvd fruM 0 por wnt, bul , ni is ciLilnmary in peaifeide I'.natysv/, l!.-' c*i *I . of tSortliiiul. i.i'iiplrt of bliibU-r welt; uviiiluble no correcti'<n lias bren made 1er sudi lu/vs in the ie.-ults L-r t.!l i . f : i.'ciiixMi in.iolined, anil hi n few cnrvs other presentili hrir-. or;- in i.? ai>.i vnit:::,i.>. Ait well c i rcw,'ui'.i,(l jiii.li'jiilu T ho i-h-uiitt-up lu-xmio extracts were analysed by g:u - r -.d j..:, i.'hvr eb tirmi t iiiiuriiiy Mil-ilnm-'.n lm \o l-en liipiid rlirviuiiU-'.inlthy nil n Vm:m 'Aernuraph m,"i-/>' "f i-.i t Iti.-iit', ei'iuni.Uevrej.liy m.ii, in ouh r tu iiiiiniiu-.'ti!-finpl'.'yti iw o gUiM eo h iiiim 5 f:. eng x in. * '* * ' r -h ... .e *'*'in . n i* iil- iv i!!.-:riSuleil mit-'i- lii;*.*.'"! e. tini re!m .i:i \.v t p.tehi*d ::i "Chromo* '*' 1 *' !' -'M-id'-r., b!':' 1,-r .:imo!i.i; frui;i gu-y ivtiln nod ri irli, llf .* SC-HW' m i-h r e iile ii *vi:Ii 10 jkt Cent mb C1K1**-'1 wit nilr'^i-N n< for O-.-Mri.i n-m t.o ci;: IVliil'i ev'f rvilthte:l h iu!e*i'Tom: with J'./'anidylicid vm - ovnp and tho sleoloilio walor-bnt wiiti mix ulpliato tticn com tcclinifpti nr tabled, ft the origi1 and p,J>* from tin in tlir-so whore l affected, been usi hit orfen Tho i exrvmlrv In muc lower e 8eottW tions o! tho DC - by n i' 5 poi Canid DDT about 30 po uiab! * mater value; forvni ly.ced notie comb W T r.Itntl X.ItKil t.MM Cad* CaS*vi Itr Se*t r-r r i ttv At v< Rv In R( r NfcV. 0 3 1 0 ------------- 780626; ; *.C m* la? lib' V* Jig III' 1 in'* N/ Vs 1 in-1 7 in** in* C lltCS of |\tiont cf thrcu^.S Ubvlouriy v t live tfomplr i D ofil-'f 1 i*mr.rii ere froxc Ciutndir. t, vkk nftlviic* Ji-'li f )>i*slicnl und :o i.'.lo (Ai WU Ti i;nd fc.ttn <chromi i liHri'l'i't' ".i*i IKiv.* i*w mi.ir.t:.. *;; >>>, - ,..... ... ............ . ........... . -* - f.ir il:>*lil 'iti r'ptivM -n*. t/> !, ,,,, tlit->it*Iir-ii! Vin-m hi\y |i<ii fmtpir/4 ffnui | | (f` J- b: l I v.,i* llM ill*. *I>!. *.! *11 t* DJ! l>!l lirn riihi'*>M*ia rnii'ii'iitun:* !. l-l Ih>* llir*>l (ul:cn <>n IIn. ri.'ul ( > , . t.f \VliU C'iiNpl* 1 M*|*.ti.. i.iii r tlm eon'.nmn Svitll<iiii{ li't.i tii>.|!>riil\ lti|'iit*r fi.lilt tilh a! all (he r.i*Ji:^s < |tii!Wu *'** ` -n* ,,r in Ilei* 'ti li*i,i ciUtii'i*-, iii'-ii-ait.'d. tU,v i ***.!.i .linril u totnl Ji i.idtij ton- iiicrii'r* fi ia (ilyi'lilviimii* 1li)lwnyl (l'i'l'.J rvt.itlue*;*** L*i .n:i'.ujn (d: M ill mul JiiVt* ;*pjup) ri* VJ*;i p.p.rn u ' i l l t d i ni ;<>ih:i nttvN-iinird n i'umIiit An.ily#v*4 uT it feu* tiv.iM*H t>(hr*r Uum llm bbiiilvr hrtvo I minivi M'iti fi:*''. Alii'jni'in ( mi.) ni whoa 1` ll.i* vMrnr.U bet uunloi bill ti.-viceniritliont in tbi: b|ul>|/*-r iuivi* i.ltinyi* n'i-lt) Vi*JMrnl'd lo diy***:' i:t >r.ln:nm *'. v*l'l lill*;vrl n*r, b.vit (Aimd to 1"' il)A ltf/hvti (TmIiIc ). (iuvTim,.i'*n`: in unii lln* nvilttf* rolliv d vlt 2 mi. f ii*>* |ier ni oilier tbatioH em u liut-lv lo lw ruiiMiaU'iil lit lln*ir |.m \r ,i|(-(ihnli(i iiviir.'K^i* fnr irtin-nii" ii`'%ri!i " lipid conlrnU. V , " , | | I I . A i t l T p > 'i i l ' , I t t i , f fl* lll* X llItO II*.IN I t d J ' . 'd , -- 's n'illi mixiiijf, liillo'VisI l.y .ti ti,l. Al' 2 per (Viti Nudimi) Ialite r U i n*r co' c r n k it io m (r-r-m-) rx TMirses ti* *n< fkitl|*liuUi .<i<l(ii*in Tlh.* Mti:>t'n>:ii:uil hi'Mtini }navi*r vno ihvi* ('unltvi'il l*y fiiH-Ii-jiiM i*l>rK>iU'i:ntjilty. Uy liti*-, t<*dini>li:, qiiniiiititiive convenivim ni /a/i'.n i)T lo /,;' J)1)JC, mul of ;v**T 'K io ,:ap'*MlH5 (DtM UL ia ob*. t.inrd, nini ilio touvem en pr.n:ucts cunlrin t he idem li v uf ilio origina1. ri i<bu . The ro.*.hlual puntili li iho j.p'.D D T Aanipto tiif fii.-j-f*;.! Un** lin y t-u\ ll)i.t*l*er (> VI UK S^ 3-1 ) U*er C 77 V-3A n-er it ta O il 0-Sft Jlr.tln 0 -?A or. OCI 0-17 ot>: C 0.` KNntf ta ta -- _ 001 ta 03 (HI fru frJQ mm r_ Q-M nml p,)'-TDI i'o^lion*i, v iv r proseut, m u ic miU me ivii fremi ilio vaino. n f ilio j u n i e * ri;:inn(Iy dcu-nniurd in ilimu yr/v!: iu.>. nli-fi iltMirny* a nitd^lilTC , whora prasent, Ina- pmyvii'.' nateti biphcityls oro un* )i pfTeoved. A oyiinoKilicoHi (A'/,*&0) colmila w inni almi buon iwetl lo k'f'p.UMto j'.j/O -O T unti J3 frotn l 'CU \ intor.bronco, coiinttin^ lim lau cr. I Tuo priucipnl t*C4lIcido remiIiics round in (ili smnplc* 1 tsaminod vote titolo ol* tho DDT group, v illi dicMrin in muoh mailer ciuniiliticA. Canatiimi f-cnl cmt niocil I {ver conconrrntiomi c f dicldrin ihnn Scott uh acnln, vlitlo \ Scuiti/ll porpore* comaine lo.'iiarltnbty iiipii concoiura* doni* of nil pi'jUVide r.sif'.ucs (km Tnblo 1). iiirorforcttco on ii (l.o /lC/20Of.i',ic.uto folunmir. lhop.p'*TJLiI poziiion cntiKcd by u non-liytirtiiyitablc rojino vn* j'-ipojiit)!c' ior about* 5 per cui.i. of tho total c.ittnmted p,p'*TI)E in ilio Conadimi nini Scollili tr.i:t\ii>A cxrtmiucd. In tho p,;A PD1' p>}ititir,nt tho non*hyd.*olyalilo ro.iidoo coustitmcd aboui 10 par cent, in the Ci:miriinn rair.plrs ami about 20 per cent in tho Scottigli camplcs cxnmircd ior thi* ialt>i'for;co, ilio loia u.ivn;nl o f imn.itydrolypablo nieterial beili? <*m;'.'.!cr in tnn C.\nodimi f.i:.ip!cu. Tiio valiiui in Tablo 1 Ijiivo not beati vorrcctcd l'or il tiucr* tciviicc, ivir oniy a proporucn tu" iu* wi!ii|"',r* muu , iv Tho proporIion of blubber oxlrnclablo by Itcxnno vnt detenninrd for ?uno minplo. Vnlw.** ranged from 72 lo xG |K*r cent (turnii 'j per cunt-) for tho Cnnndtan m-qIn, and from SS lo ttd pnr rv.nt (inonit Vi per coni) for Scollich mais irmnpleit on tho bricding ground off the vent cocui. (Values for other pcul rampici vere not ottimo'ed.) Con ceutration of prst trie! in term of cxtracthblo fat for thcAo Mvniplcii vould Ihua bo up to 40 par cent greater than tho vaimi* in Table 1, but tho general romnnrison ii not ignincanlly affected. Tho lover extractable fut value for tho Canadian rampU* may possibly bo a result o f being preserved in forialin. Concentrations of pentititi* residue in tho oxtroctablo fat of various organs and tisaut-s of one porpoiao show an imiKirUmt ix*l.itioivjliip (Toblc 3). \Ytuto Uto concentra tions of tho DDT frruip in tho oricinal tissues vury widely, tho^o oxpreyed in tenm of cxtracmblo fat arc in much cltMcr ngreementt vi:h tho colo exemption of llm brain. A similar auonwily f*r tiswro of lTnt v a s dcarthrd by* Htilden1*. I t sccrn.* lihry that n 1-irgo prorortion of tho lipid fraction of rd auiy not'- contain veil cides, and tho brain lipid ro*r.p*/.iition it known lo differ considerably fm iti that, o f dr.iit fat lyrcJ. tti-.ilr? iVom ibe vari**ui rpo.iii.tit o f ih-iiI i vero not tuiticcahly diiVcivrit in n givou arot, and hnvo beoti combincd in Ti.blo ). Ttlv i , c<3NCi;N7RiTiax itixir-t o r er.CANOJwtautvi hujidcxc n UT or in .tu a i * r .^ ro u r j (r>.r.t;t.) (Hunt ir. i.sreoil(em) Tib) J, c o i r r s t i s M t o s or T * t p o t (p.p.m.) i x t p i c s a o r s c x f o ib TIu'jc or (iiv-ui Walbit Mir.xlf Liver 8fha Kidney Onla ConrenltMlnuln tn*tw p/ri-'tii.i^otx iiK l. 7-0 0-06 M JC3IH Coanlnileer>ttiftMit-BIn s-o 9`t ( frit SI 14 out l* frM .n to O-JT exttcclaUo (>l Ares nnJ Xo.ln f4**.r)bE p.p'.fPE H. SvolLtnil. i fita (IW*i-C*l| X.indMutWf -..\SIe{nl^t*Jtiu0l.\*) X. o:.J `iV. Aa*t:^titl, val ta:*J(>:*"l*i> 1$ C V O-J(CSV--C.-'.Mt Otr-OCIQ0W---f1CrS-*rS-)Ot Cablila, SU-j.t iU-nIt.. Juv.and&Jnit ral* X ocCdfr-0o3>rt Cat]Mrti^;s*vf-itt.Ji,|anI.rICV.tma*bl*7.lt:u) nt>U f 0^(30-*00*71)0 SL.*.arJ*d;.vu^tlntldaM,i-nli<.icn**ry(,1U *) pot^.u-allvJX l&C?) s 9 ero 4*t-IS-0 (fr> 1*tf0i--S11I*1 1-S-T-O l-0(a5--*40t-)0 0*2i7i*-3;*>0 0-17-frei o-i(:0.-*t^n (C*3il (1*3) tO*lfi) >2-17*3 (VI) 03V-5-1 (O-Tf.) 1-1 013 frfr-n- Uif) ' S-(t-*H0)* r,y**))I>T V(7V-e7>-7 1-(13-131-7 0T..<-.>3 (O-Sdt M-ivn (SO 21 is-i-tVT t=m) V* l*};o \widentified pcnl'j on the cliromaiograni seem to bo similiiT to thoio produced by polyehlorinalcrl bi phenyla and, to cnublo eompnrison to he made, the ratios l/fa) of the retention times of nil regularly occurring peek* (relit(ivo to liieMriniT IPO) hovo biM-n cnlrululrd for tho ojKTiitins column lempentluro .Pf 200' C, thcuo vnlt;t * being tennirriitim-deix'mlcnt. *Tho Itx values of PCII compounds from romnhTcinl PC11 foimui.iiic-ns vorv also determined for both types of column Table 4). At least oven I'CH-lype penhR vrro found, including tlu?o vhieli interfere with p.p'-TDTS and p.p'-DDT. Itcptnchlor d>oxido nml could not bo confirmed on both columns ami n o not thereforo recorded. Ctmtamlnalion by peitiridm greater in tho reals on the east coast c f fcc'olhwul (alien in an area roughly from Aberdeen to tho Toy estuary) tl;an in spv'oiini'ins from lho vv.it and north eonv.s, iaeliuling tJio Orkney islands. Seal pupt from tho Orkney and Harris ('vr.it co.*.*tt) breed* in? grounds eoivtniucd oi.ly lipiilly lov.vr re-'tW-iofl tlinn ednli>i fr,>ni the anno arc*1. IJv roirptirison. ihv Cnntulum MinpleN from the Mr.^wi.i.,r. (Oulf of Si* Lawrence) Tho ino.L Rigniheant ntipcet* of tho results is that, ulthomjh seals mu; piirpoise* tnhabit an envirt>nu:''nt for ronioved fon* tlu* silo f application or diKchnrga o f pcKtietdea. they c.in cun*/tin tnurh greater eonerr.iraiitas of divhirm ami J>T/r gri ttp rcvlducx titan llioso recorded for inosl oilier t peeies, includm? r^in. llobinxon Humor1* fouiid r.-inMia coitccntmtion of dtoMrin oT fi*22 p.p.m. (ra;tgo o*ll'-0*73. p.p.rn.), and a ii.cv.n total con- NEV 031039 780627