Document jRZQg1wgv7JoG4eLwqbq9vZ9

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: [2-(N-Ethylperfluorooctanesulionamido) ethyl acrylate; may also be referred to as B1228, D-1, EtFOSEA, or FX-13. (2-Propenoic acid, 2[ethyl[heptadecafiuorooctyhsulfonyllaminolethyl ester, CAS # 423-82 5) . Remarks: Material is an amber solid. Sample purity and not recorded. 3M lot number 13. METHOD Method: Protocol Reference EARWAS 5/9/84 Test type: Static acute GYeLaPr:CoNmopleted: 1984 Analytical monitoring: Dissolved oxygen concentrations. Test organism source: Activated sludge collected from the St Paul/Minneapolis, MN Metro Wastewater Plant on 5/10/84 Test coDnidliuttiioonnsw:ater: Deionized water Test solution preparation: Placed 0.30 g test substance in a 300 mL BOD bottle with 50 mL deionized water. The mixture was mixed with a magnetic stirring bar and box for ~ 30-minutes in order to disperse sample. After mixing the sample was finely suspended in the water. Test vessels: 300 mL BOD bottles Number of concentrations: One `Temperature: Not recorded Total Suspended Solidsof sludge on day of testing: Not recorded. Sludge was used the day of collection. Exposure period: 6.65 minutes Element Basis: Respiration inhibition as determined by dissolved oxygen concentration. RESULTS Nominal concentrations: Two blank (deionized water) controls, one 1000mg/L test material solution. 000541 Statistical Analyses: ECs values was not determined as the oxygen depletion rate for the controlswasjudged to be equal to that of the deionized water. controls Analytical Methodology: Method for the analysis of dissolved oxygen concentrations was not recorded. Control response: Satisfactory; difference between controls was 6.9%. CONCLUSIONS _ The test substance exhibited a no inhibitory effect on respiration at a nominal test substance concentration of 1000 mg/L after 6.65 minutes of exposure. The ECs (respiration inhibition) is therefore greater than 1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. This study was not conducted using an established method. Testing was also done well above the solubility limit of the test substance. This study lacks sufficient documentation and characterization of the test substance purity, and analytical measurement of test concentrations. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number B1228, 4/1/84 OTHER Last changed: 5/22/00 000542 > 5 aC 3M Environmental Laboratory Acute Toxteity of a Substance to Protocol RAecfteirveantceed: SlEuNdRge/WAMSixe5d/08L/i8g4u,or L(oSbLURDeBqE-uTeOsXt) No. B1228 8 7 6 T s FE = 5p . =8 5 5a 2BE : a 2 1 Legend |O DEIONIZED WATER CONTROLS - AVERAGE |A B1228-1,F-13,LOT-13, (1000HOA) N A 1500== > >1000 M//L -\ N\ A \ . \ A \ 0 v1 STOX.DAT 3 3 1 F TIME - MINUTES - 000543 %7 ash" [8 Tor BEST COPY AVAILABLE Protocol Reference: Eapsas 5.09.84 = } 3M Environmental Laboratory Acute Toxicity of a Compound (Substance) to.Activated Sludge MixedLiquor BLarbirequest no.: _f3/20% ip vatoen: eSLC JS amatysei lh lida The activated sludge mixed liquor was collected from the St. Paul/Kinneapolis Semple description: (L/225-)) Fy-i3 Lo280Y FMYSIG, LoT-13 (Ha Teaser water GTS See Comin Aad porn otoEparEPlEe were 41 Medto ___. m [am ieEc.| Boke ao OF TEST MATMATERI, I als DSLAC ARE 7EP I 7 . 2EZEeI I Pe I Ea ereTTT To Bl eorlaer| 1 oe [oo| Fm A EieEe lsosr TheeeeT -- TTTooo-- err]] 8el e ed e et ] COMMENTS: CTaRo Eaettin~ETE Luni D005Liporkplenatmor plTohcalecen war phos OL ihe iyi oD a igs stinaty bo and Lox fr 30min am acl He AioperaeGonmphs CFT pag, He Aenpls cas firth suspen Ha wate, 000544 .