Document jNyzLLKGEyKLYyQry0nyRvg2R

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 1 Laboratory Services and Applied Science Division 11 Technology Drive North Chelmsford, Massachusetts 01863-2431 Drafted Date: Finalized Date: July 27, 2021 August 24, 2021 Subject: Reconnaissance and Sampling Inspection Report Clean Water Act - National Pollutant Discharge Elimination System City of Worcester, MA From: Michelle Coombs, Inspector Field Services Branch (FSB) Laboratory Services and Applied Science Division (LSASD) \MRC\ Reviewed By: Jerome Keefe, Investigations Team Leader, LSASD-FSB \JCK\ To: Denny Dart, Water Compliance Section Chief, Enforcement and Compliance Assurance Division (ECAD-EWC) CC: Douglas Koopman, Inspector, ECAD-EWC I. Facility Information A. Facility Name: City of Worcester, MA B. Facility Location: 20 East Worcester St, Worcester, MA 01604 C. Facility Contact: Jay J. Fink, P.E. Commissioner, Dept of Public Works and Parks, (508)929-1300, dpw@worcesterma.gov D. NPDES Permit ID:MAS010002 (MS4) II. Background Information A. Date/Start time of recon inspection: July 13, 2021/8:50 AM B. Weather conditions: Cloudy, approximately 65F C. USEPA Representatives: Michelle Coombs D. State/Local Representatives: None E. Date/Start time of sampling inspection: July 14, 2021/8:45 AM F. Weather conditions: Cloudy, approximately 70F G. USEPA Representatives: Michelle Coombs & Shannon Brunelle (FSB) H. State/Local Representatives: None I. Federally Enforceable Requirements Investigated: Stormwater discharges and certain non-stormwater discharges from the City of Worcester, MA (the City's) Municipal Separate Storm Sewer (MS4) are authorized by the National Pollutant Discharge Elimination System (NPDES) General Permit for the Discharge of Stormwater from Small Municipal Separate Storm Sewer Systems (hereinafter, the Permit), issued by the Environmental Protection Agency (EPA) City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Region 1. The City obtained coverage under the Permit with NPDES Permit ID MAS010002. J. Pollutants Sampled: Pharmaceuticals and Personal Care Products (PPCPs), E. coli/Enterococcus, Ammonia (nitrogen), Total Chlorine, and Surfactants III. Disclaimer: Unless otherwise noted, this report describes conditions at the facility/property as observed by EPA inspector(s), and/or through records provided to and/or information reported to EPA inspector(s) by facility representatives and as understood by the inspector(s). This report may not capture all operations or activities ongoing at the time of the inspection. This report does not make final determinations on potential areas of concern. Nothing in this report affects EPA's authorities under federal statutes and regulations to pursue further investigation or action. IV. Type and Purpose of Inspection(s) The purpose of the reconnaissance inspection was to locate stormwater outfalls, associated manholes, and/or catch basins which discharge into the Fitzgerald Brook, Beaver Brook, Poor Farm Brook, Ararat Brook and Tatnuck Brook, while noting appearance, presence of flow, and access requirements for one or more sampling inspections. The purpose of the compliance sampling inspection was to identify potential illicit connections or illegal discharges within the City of Worcester, MA MS4 that may adversely impact the water quality in the Fitzgerald Brook, Lake Quinsigamond, Beaver Brook, Poor Farm Brook, Ararat Brook and Tatnuck Brook. Samples were collected from twelve (12) locations in accordance with the FSB Investigations Team Stormwater Program Plan. Sample sites were identified during a reconnaissance inspection conducted on July 13, 2021. V. Permittee Details The City of Worcester occupies approximately 38 square miles in Worcester County, MA, approximately 40 miles west of Boston, MA. The City shares borders with the municipalities of Auburn, Grafton, Holden, Leicester, Millbury, Paxton, Shrewsbury, and West Boylston. According to the 2010 U.S. Census, the population of Worcester was 181,045. There is 1 CSO permitted to discharge into the receiving waters of Mill Brook (MA0102997). VI. 07/13/2021 Reconnaissance Inspection Summary On July 13, 2021, Michelle Coombs conducted an unannounced reconnaissance inspection of the Worcester, MA MS4. She was not accompanied by any city or state representatives. The inspection started in Fitzgerald Brook at approximately 8:50 AM. At the time of the inspection, the weather was cloudy with an ambient temperature of approximately 65F. According to Weather Underground for the Worcester Regional Airport (KMAWORCE80), the last precipitation event prior to the inspection occurred on July 12, 2021, with 1.01 inches Page 2 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection recorded. Approximately 1.79 inches of precipitation was recorded within the 72 hours before the inspection. None of the locations visited during this inspection were field screened with the test kits or the YSI quality meter. Locations visited were noted for appearance, presence of flow, and access requirements. Photographs taken during the inspection are listed in Appendix A. VII. 07/14/2021 Sampling Inspection Summary On July 14, 2021, EPA employees Michelle Coombs and Shannon Brunelle conducted an unannounced compliance sampling inspection of the Worcester, MA MS4 at the locations described in Section VIII. They were not accompanied by any city or state representatives. The inspection started at Fitzgerald Brook at approximately 8:45 AM. At the time of the inspection, the weather was cloudy with an ambient temperature of approximately 70F. According to Weather Underground for the Worcester Regional Airport (KMAWORCE80), the last precipitation event prior to the inspection occurred on July 13, 2021, with 0.02 inches recorded. There was approximately 1.31 inches of precipitation recorded within the 72 hours before the inspection. According to the National Weather Service, in the month (to date) of July 14, 2021, the City observed 8.57 inches of rain, which is a 6.86-inch departure from the 30-year normal value of 1.71 inches. According to the US Drought Monitor, as of July 13, 2021, Worcester County is experiencing no drought conditions. Due to time constraints, the sampling locations described in Section VIII were not field screened using test kits for ammonia (nitrogen), surfactants, or total chlorine. In-situ measurements for specific conductivity, salinity, and temperature were measured using a YSI water quality meter and recorded. Field data were recorded on field data sheets. Samples were delivered to and analyzed for E. coli and Enterococcus and PPCPs at the EPA New England Regional Laboratory (NERL) in North Chelmsford, MA. Table 1 below summarizes the sampling and analytical data. Photographs taken during the inspection are listed in Appendix A. VIII. Description of Sampling Locations Photographs depicting each sample location are included in Appendix A of this report. LQ13A: A single clay outfall approximately 12 inches in diameter, flowing into a channelized stream located at 240 Coburn Ave. Discharge flow was approximately 810 gallons per minute (gpm) into Fitzgerald Brook. A musty odor was detected at the outfall. Sample was collected at the mouth of the outfall. LQ13: In-stream sample of flow collected from channelized stream at Fitzgerald Brook inside of the stormwater gate, prior to the confluence of the LQ13A outfall. A strong musty odor was detected, and trash, including rags, was observed downstream. BB4: A single steel outfall approximately 24 inches in diameter, located at 645 Park Ave. Discharge flow was approximately 30 gpm into Beaver Brook. Sample was taken from the mouth of the outfall. White suds were observed floating in the brook from upstream. BB10: A pair of reinforced concrete outfalls approximately 36 inches in diameter located on the northeast side of the bridge at the intersection of Maywood St and Beaver Page 3 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Brook Pkwy. Discharge flow was approximately 50 gpm into Beaver Brook. Sample was collected from the mouth of the southernmost outfall. A musty odor was detected. White suds were observed floating in the brook from upstream. BB13: A pair of reinforced concrete outfalls approximately 36 inches in diameter located on the northeast side of the bridge at the intersection of Maywood St and Beaver Brook Pkwy. Discharge flow was approximately 50 gpm into Beaver Brook. Sample was collected from the mouth of the northernmost outfall. A musty odor was detected. White suds were observed floating in the brook from upstream. BB19: In-stream sample of Beaver Brook collected where Beaver Brook daylights at 300 Chandler Street. A slight musty odor was detected and trash, including rags, was observed downstream in the channel. Some white suds were observed floating in the brook from upstream. MillTB: In-stream sample of Tatnuck Brook, collected downstream of the Mill Street bridge after the waterfall and two MillTB outfalls below. The water color was slightly brown and turbid. A slightly musty odor was detected and a significant buildup of white suds and brown algae was observed along the left bank (looking downstream). MillTBS: A pair of reinforced concrete outfalls located on the northern bank of Tatnuck Brook downstream of the Mill Street bridge. Southernmost outfall is approximately 12 inches in diameter. Discharge flow was approximately 10-15 gpm into Tatnuck Brook. A slight petroleum odor was detected, oxidized iron precipitate was observed on the reinforced ledge of both outfalls. Sample was collected from the mouth of the outfall. MillTBN: A pair of reinforced concrete outfalls located on the northern bank of Tatnuck Brook downstream of the Mill Street bridge. Northernmost outfall is approximately 24 inches in diameter. Discharge flow was approximately 20 gpm into Tatnuck Brook. A slight petroleum odor was detected, oxidized iron precipitate was observed on the reinforced ledge of both outfalls. Sample was collected from the mouth of the outfall. AB07: A single reinforced concrete outfall approximately 24 inches in diameter, located adjacent to the residential property at 116 Holden Street. Discharge flow was approximately 20 gpm into Ararat Brook. A musty odor was detected and green algae growth was observed inside the pipe. Sample was collected from the mouth of the outfall. PFB15: A single concrete outfall approximately 30 inches in diameter located on the north side of 326 Clark Street. Discharge flow was approximately 30 gpm into Poor Farm Brook. A musty odor was detected and dark green algae growth in the pipe was observed. Sample was collected from the mouth of the outfall. CCBlvd: Sample was collected at a stormwater structure along Country Club Blvd at Poor Farm Brook, near the intersection with Shenandoah Circle. Flow from the brook was cascading over a ledge, and there appeared to be an outfall also flowing, but it was directly under the flow of the brook. The sample was collected from where the outfall appeared to be flowing. A slight chlorine odor was detected. Page 4 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Map of Sample Locations Note: the locations on the map were estimated using Google Earth and are used for display purposes only. Page 5 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Table 1: Summary of Sampling and Analytical Data Location Coordinates1 (decimal degrees) YSI Meter Field Test Kits (mg/L) Site ID Sample Date Sample Time North West Salinity, ppt. Temperature, C Conductivity, S/cm Ammonia Total Chlorine Surfactants LQ13A 07/14/2021 8:40 AM 42.263966 -71.759445 0.1 16.7 200 NA NA NA LQ13 07/14/2021 8:50 AM 42.263947 -71.759442 0.2 16.9 408 NA NA NA BB4 07/14/2021 9:20 AM 42.246054 -71.833607 0.6 19.3 1,193 NA NA NA BB10 07/14/2021 9:50 AM 42.253803 -71.830239 0.3 17.0 696 NA NA NA BB13 07/14/2021 9:55 AM 42.253820 -71.830391 0.3 16.7 687 NA NA NA BB19 07/14/2021 10:15 AM 42.261794 -71.824230 0.2 16.9 437 NA NA NA Bacteria (MPN/100mL) E. coli Enterococcus 58 774 >9,678 >9,678 9,678 2,069 <10 241 213 813 620 426 Cotinine ND 8.5 20 17 22 3.3 Acetaminophen ND 46 190 68 75 170 Paraxanthine 3 47 110 130 160 72 PPCPs (ng/L) Atenolol Caffeine ND ND 12 8.7 9.1 5 4.7 55 10,000 230 290 120 Metoprolol ND 6.9 21 5.2 5.4 4.8 Diphenhydramine 0.73 0.93 15 3.9 4 2.1 Carbamazepine ND 2.2 1.3 5.3 4.4 1.3 Abbreviations and Notes: 1. The coordinates listed in Table 1 were derived from the map created in Google Earth, therefore the coordinates are estimates of the actual locations. N/A: Not Analyzed ND: Not Detected above Reporting Limit E. Coli: Red 10,000 col/100ml, Orange 1,260 col/100ml, Yellow 235 col/100ml, Black < 235 col/100ml Entero: Red 1,000 col/100ml, Orange 350 Yellow 61 col/100ml, Black < 61 col/100ml NH3: Red 6 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L Cl2: Red 1.0 mg/L, Orange 0.3 mg/L, Yellow 0.02 mg/L, Black < 0.02 mg/L Surfactants: Red 1.0 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L (may give false positive at salinity greater than 1 ppt) PPCPs: Dark Pink 100x the RL; Pink 10x the RL; Light Pink 3x the RL; No Pink < 3x the RL* *See Reporting Limit (RL) values for each compound in attached Laboratory Report for Source Tracking EPA notes while there are currently no numerical standards to compare pharmaceutical results against, it is EPA's experience that acetaminophen is the single best bacterial source tracking compound of those listed above, and any detection of this compound may indicate a source of sanitary sewage. With respect to all of the above compounds, when a sanitary sewage source is present, depending on the type of source, distance from the sample location, and the strength of the source, concentrations of these compounds may range from the low ng/l range up to thousands of ng/L. Page 6 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Table 1: Summary of Sampling and Analytical Data, cont. Site ID MillTB MillTBS MillTBN AB07 PFB15 DUP01 CCBlvd Location Sample Date 07/14/2021 07/14/2021 07/14/2021 07/14/2021 07/14/2021 07/14/2021 07/14/2021 Coordinates1 (decimal degrees) YSI Meter Field Test Kits (mg/L) Bacteria (MPN/100mL) Sample Time North West Salinity, ppt. Temperature, C Conductivity, S/cm Ammonia Total Chlorine Surfactants E. coli Enterococcus 10:42 AM 42.274592 -71.853855 0.1 18.6 220.5 NA NA NA 212 171 10:50 AM 42.274677 -71.853826 0.8 18.8 1,651 NA NA NA 4 <10 10:56 AM 42.274713 -71.853872 0.3 17.2 700 NA NA NA 346 272 11:30 AM 42.307948 -71.819324 0.5 17.1 969 NA NA NA 248 10 11:55 AM 42.312472 -71.774554 0.2 18.0 457.9 NA NA NA 774 399 11:55 AM 42.312472 -71.774554 NA NA NA NA NA NA NA NA 12:20 PM 42.300584 -71.776867 0.2 17.7 507 NA NA NA 1,102 350 Cotinine 1.2 0.41 1.9 1.2 9 9.5 3.5 Acetaminophen 33 ND ND 8.5 48 64 20 Paraxanthine 9.8 5.2 ND 6.9 48 58 19 PPCPs (ng/L) Atenolol Caffeine ND ND ND 4.1 5.5 6 7.7 9.6 7 5.8 13 170 190 93 Metoprolol ND ND ND 1.8 16 17 4.3 Diphenhydramine ND ND ND 0.74 6.2 6.6 2.2 Carbamazepine 1.4 0.52 1.9 ND 12 13 4.4 Abbreviations and Notes: 1. The coordinates listed in Table 1 were derived from the map created in Google Earth, therefore the coordinates are estimates of the actual locations. N/A: Not Analyzed ND: Not Detected above Reporting Limit E. Coli: Red 10,000 col/100ml, Orange 1260 col/100ml, Yellow 235 col/100ml, Black < 235 col/100ml Entero: Red 1,000 col/100ml, Orange 350 Yellow 61 col/100ml, Black < 61 col/100ml NH3: Red 6 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L Cl2: Red 1.0 mg/L, Orange 0.3 mg/L, Yellow 0.02 mg/L, Black < 0.02 mg/L Surfactants: Red 1.0 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L (may give false positive at salinity greater than 1 ppt) PPCPs: Dark Pink 100x the RL; Pink 10x the RL; Light Pink 3x the RL; No Pink < 3x the RL *See Reporting Limit (RL) values for each compound in attached Laboratory Report for Source Tracking EPA notes while there are currently no numerical standards to compare pharmaceutical results against, it is EPA's experience that acetaminophen is the single best bacterial source tracking compound of those listed above, and any detection of this compound may indicate a source of sanitary sewage. With respect to all of the above compounds, when a sanitary sewage source is present, depending on the type of source, distance from the sample location, and the strength of the source, concentrations of these compounds may range from the low ng/l range up to thousands of ng/L. Page 7 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection IX. Appendix Appendix A: Photograph Log X. Attachments Attachment A: Laboratory Report for PPCPs (Source Tracking) Analysis Attachment B: Laboratory Report for E. coli Analysis Attachment C: Laboratory Report for Enterococcus Analysis End of Report Page 8 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Photo Name LQ13A & LQ13 LQ13 Down LQ13 Down (2) BB4 BB10 Down BB10 & BB13 BB19 MillTB Down MillTB N&S AB07 AB07 (2) PFB15 CCBlvd CCBlvd (2) Appendix A Photograph Log Date Taken 07/14/2021 07/13/2021 07/13/2021 07/13/2021 07/13/2021 07/14/2021 07/14/2021 07/14/2021 07/13/2021 07/13/2021 07/13/2021 07/13/2021 07/13/2021 07/13/2021 Time Taken 8:35 AM 8:56 AM 8:56 AM 9:17 AM 9:30 AM 9:56 AM 10:10 AM 10:38 AM 10:07 AM 10:24 AM 10:24 AM 10:52 AM 11:14 AM 11:15 AM Page 9 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection LQ13A & LQ13: Outfall located on the west side of the street at 240 Coburn Avenue. "LQ13" sample collection point indicated by a red "X", collected upstream of "LQ13A" and the confluence of Fitzgerald Brook that flows into the gated stormwater structure. "LQ13A" sample collection point indicated by a red box. Flow direction indicated by a blue arrow. Page 10 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection LQ13 Down: Photo taken on the east side of the street at 240 Coburn Avenue. Fitzgerald Brook, downstream of the LQ13 & LQ13A sample collection points. Note the large amount of white suds on each side of the flow path. Page 11 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection LQ13 Down (2): Photo taken on the east side of the street at 240 Coburn Avenue. Fitzgerald Brook, downstream of the previous photograph. Note the trash along the banks of the brook. Flow direction indicated by a blue arrow. Page 12 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection BB4: Outfall located at the intersection of Mill Street and Park Avenue to the south of Advanced Auto Parts parking lot. Sample collection point indicated by a red arrow. Beaver Brook flow direction indicated by a blue arrow. Page 13 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection BB10 Down: Outfalls located at the intersection of Beaver Brook Parkway to the southeast. Outfalls are located under the bridge, indicated by a red box. Beaver Brook flow direction indicated by a blue arrow. Page 14 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection BB10 (South) BB13 (North) BB10 & BB13: Outfalls located under the bridge at the intersection of Beaver Brook Parkway to the southeast. Sample collection points indicated by red arrows. Page 15 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection BB19: In-stream sample of Beaver Brook located at 300 Chandler Street. Sample collection point indicated by a red arrow. Flow direction indicated by a blue arrow. Page 16 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection MillTB Down: Downstream view of Tatnuck Brook along Mill Street with the "MillTB N & S" outfalls indicated by a red box. In-stream sample of MillTB collected just outside the bottom-right frame of the photograph. Page 17 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection MillTB N MillTB S MillTB N&S: Outfalls located on the northern bank of Tatnuck Brook downstream of the Mill Street bridge. Sample collection points indicated by black arrows. Page 18 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection AB07: Outfall located adjacent to the residential property at 116 Holden Street, indicated by a red box. Ararat Brook flow direction indicated by a blue arrow. Page 19 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection AB07 (2): Outfall located adjacent to the residential property at 116 Holden Street. Sample collection point indicated by a red arrow. Page 20 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection PFB15: Outfall located on the north side of 326 Clark Street discharging into Poor Farm Brook. Sample collection point indicated by a red arrow. Page 21 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection CCBlvd: Sample location along Country Club Blvd at Poor Farm Brook, near the intersection with Shenandoah Circle. Sample collection point indicated by a red arrow, where there appeared to be an outfall with flow beneath the flow of Poor Farm Brook. Page 22 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection CCBlvd (2): Downstream view of the Sample location along Country Club Blvd at Poor Farm Brook, near the intersection with Shenandoah Circle. Page 23 of 26 Sampling Inspection Date: July 14, 2021 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Attachment A Laboratory Report for Source Tracking Analysis Author: LSASD Laboratory Services Branch Pages: 20 Page 24 of 26 Sampling Inspection Date: July 14, 2021 July 28, 2021 Michelle Coombs - LFSB US EPA New England R1 United States Environmental Protection Agency Laboratory Services and Applied Science Division 11 Technology Drive North Chelmsford, MA 01863-2431 Laboratory Report Page 1 of 20 Project Number: 21070009 Project: Worcester, MA MS4 Analysis: HPLC/MS/MS Source Tracking Analysis EPA Chemist: Peter Philbrook Date Samples Received by the Laboratory: 07/14/2021 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP-LCMS_STA3. The SOP is based on an EPA Regional Analytical Method developed at the EPA New England Laboratory. Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8340 . Sincerely, 21070009$STA Qualifiers: RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E = Estimated value exceeds the calibration range L = Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P = The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC/MS. A = Suspected Aldol condensation product. N = Tentatively identified compound. Page 2 of 20 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 3 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ13A 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L ND ND 3.0 ND 4.7 ND 0.73 ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92835 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 77 60 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 4 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: LQ13 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 8.5 46 47 ND 55 6.9 0.93 2.2 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92836 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 77 59 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 5 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: BB4 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 20 190 110 12 10000 21 15 1.3 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92837 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 20 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 80 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 72 94 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 6 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: BB10 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 17 68 130 8.7 230 5.2 3.9 5.3 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92838 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 78 66 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 7 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: BB13 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 22 75 160 9.1 290 5.4 4.0 4.4 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92839 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 79 70 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 8 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: BB19 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 3.3 170 72 5.0 120 4.8 2.1 1.3 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92840 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 87 57 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 9 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: MillTB 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 1.2 33 9.8 ND 9.6 ND ND 1.4 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92841 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 77 57 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 10 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: MillTBS 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 0.41 ND 5.2 ND 7.0 ND ND 0.52 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92842 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 52 42 QC Ranges 59 - 137 19 - 135 Comments: Surrogate recovery of phentermine was below QC limits, the surrogate recovery of diphenhydramine was acceptable - no action taken. 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 11 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: MillTBN 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 1.9 ND ND ND 5.8 ND ND ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92843 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 75 54 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 12 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: AB07 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 1.2 8.5 6.9 4.1 13 1.8 0.74 ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92844 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 L 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 70 55 QC Ranges 59 - 137 19 - 135 Comments: L = Estimated value is below the calibration range. 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 13 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: PFB15 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 9.0 48 48 5.5 170 16 6.2 12 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92845 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 76 60 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 14 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: DUP01 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 9.5 64 58 6.0 190 17 6.6 13 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92846 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 82 64 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 HPLC/MS/MS Source Tracking Analysis Page 15 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: CCBlvd 7/14/2021 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L 3.5 20 19 7.7 93 4.3 2.2 4.4 Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: AB92847 Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Diphenhydramine-D3 Phentermine-D5 Recoveries (%) 58 67 QC Ranges 19 - 135 59 - 137 Comments: 21070009$STA U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 Laboratory Blank Page 16 of 20 Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis: Dry Weight Prepared: Wet Weight Prepared: N/A N/A 7/15/2021 7/26/2021 N/A N/A CAS Number 486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4 Compound Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine Concentration ng/L ND ND ND ND ND ND ND ND Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH: N/A Stormwater 500 mL N/A 1 N/A Dilution 1 1 1 1 1 1 1 1 RL ng/L Qualifier 0.40 2.0 2.0 2.0 4.0 2.0 0.40 0.40 Surrogate Compounds Phentermine-D5 Diphenhydramine-D3 Recoveries (%) 62 46 QC Ranges 59 - 137 19 - 135 Comments: 21070009$STA PARAMETER Acetaminophen Atenolol Caffeine Carbamazepine Cotinine Diphenhydramine Metoprolol Paraxanthine U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 MATRIX SPIKE (MS) RECOVERY Sample ID: AB92847 Page 17 of 20 SPIKE ADDED ng/L 120 120 240 24.0 24.0 24.0 120 120 SAMPLE CONCENTRATION ng/L 20.0 7.7 93.0 4.4 3.5 2.2 4.3 19.0 MS MS CONCENTRATION % ng/L REC 101 68 151 119 306 89 25.6 88 23.5 83 20.5 76 121 97 112 78 QC LIMITS (% REC) 61 - 100 59 - 122 54 - 128 61 - 120 48 - 124 30 - 130 59 - 128 42 - 125 21070009$STA PARAMETER Acetaminophen Atenolol Caffeine Carbamazepine Cotinine Diphenhydramine Metoprolol Paraxanthine U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 Laboratory Duplicate Results Sample ID: AB92844 SAMPLE RESULT ng/L 8.5 4.1 13.0 ND 1.2 0.74 1.8 6.9 SAMPLE DUPLICATE RESULT ng/L 10.6 4.95 16.9 ND 1.39 0.618 2.19 8.02 PRECISION RPD % 22 19 26 NC 15 18 20 15 Page 18 of 20 QC LIMITS 50 50 50 50 50 50 50 50 21070009$STA PARAMETER Acetaminophen Atenolol Caffeine Carbamazepine Cotinine Diphenhydramine Metoprolol Paraxanthine Comments: U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH Worcester, MA MS4 Laboratory Fortified Blank (LFB) Results LFB AMOUNT SPIKED ng/L 120 120 240 24 24 24 120 120 LFB RESULT ng/L 82.3 114 200 20.8 18.7 18.5 109 99.4 LFB RECOVERY % 69 95 83 87 78 77 91 83 Page 19 of 20 QC LIMITS % 53 - 105 62 - 117 50 - 127 28 - 126 52 - 134 30 - 130 33 - 139 61 - 113 Samples in Batch: AB92835, AB92836, AB92837, AB92838, AB92839, AB92840, AB92841, AB92842, AB92843, AB92844, AB92845, AB92846, AB92847 21070009$STA Page 20 of 20 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Attachment B Laboratory Report for E. coli Analysis Author: LSASD Laboratory Services Branch Pages: 4 Page 25 of 26 Sampling Inspection Date: July 14, 2021 August 09, 2021 Michelle Coombs - LFSB US EPA New England R1 United States Environmental Protection Agency Laboratory Services and Applied Science Division 11 Technology Drive North Chelmsford, MA 01863-2431 Microbiology Laboratory Report Project Number: 21070010 Project: Worcester, MA MS4 Analysis: E. Coli Defined Substrate Analyst: Jack Paar Date Samples Received by the Laboratory: 07/14/2021 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in. Sample preparation and analysis was done following the EPA Region I SOP, TC/ECColilert4. Sample preparation and analysis was done following the EPA Region I method: Total Coliform/E. coli by Defined Substrate. Data were reviewed in accordance with the internal verification procedures described in the EPA New England OEME Biology QA Plan. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8604. Sincerely, Jack A. Paar, III Biology Laboratory Manager Page 1 of 4 21070010ECMM Water Microbiology Laboratory Data Qualifier Codes J = Estimate H = Exceeds holding time I = Exceeds incubation time At = Atypical overgrowth S = Lost sample V = Insufficient sample volume TNTC = Too numerous to count MB = Media blank +++ = Positive control - - - = Negative control SP = Spiked Sample L = Estimated, result below reporting limit (RL) ND = Not Detected, result less than RL D = Lab Duplicate P = Plate counts outside preferred range FDUP = Field Duplicate collect time not specified on Chain of Custody. Field duplicate LIMS default time = 12:00AM Page 2 of 4 21070010ECMM Matrix: Stormwater UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 1 - NEW ENGLAND LABORATORY SERVICES BRANCH Worcester, MA MS4 E. Coli Defined Substrate Sample Number LQ13A LQ13 BB4 BB13 BB10 BB19 MillTB MillTBS MillTBN AB07 PFB15 CCBlvd Lab ID Date of Collection AB92848 AB92849 AB92850 AB92851 AB92852 AB92853 AB92854 AB92855 AB92856 AB92857 AB92858 AB92859 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 8:40 am 8:50 am 9:20 am 9:55 am 9:50 am 10:15 am 10:42 am 10:50 am 10:56 am 11:30 am 11:55 am 12:20 pm Number of Samples: 12 Date of Analysis 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm Compound Concentration MPN/100 mL E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate 58 774 >9678 >9678 9678 2069 212 4 346 248 774 1102 RL MPN/100 mL Qualifier 4 4 4 4 4 4 4 4 4 4 4 4 Page 3 of 4 21070010ECMM ~) ............... ........ ,r-. ,,.,.,.., ......,,n.,,,,.,,.,.._,.""'. REGION 1 CHAIN OF CUSTODY RECORD PROJ. NO. PROJECT NAME ')I 07oo /0 Wo,c.e.s\-er f, ,,r,r "- SAMP1/:C}J'~ .flv- ~I.f STA. NO. CD TIME 0 c<c u C) I rt- l'-1 b840 IX 7- ':f-, 14 ~o 3 i- lL{ OC\'Z.O X '-{ ".l-/ll{ oqs,s IX s 1/lt.l ~o 'x (,, 1/l4. \Ol5 ')( =t will lo'i'l. IX 2- 1/,l{ lOSo )( q 7-/I'{ loSCo r LO 1/M ll'30 D< l\ 7/l'{ llS.S I>< -- \. / ~J-' / .... \L t/\4 \'2.'U) I SL.\- lNon.:e~r,MA STATION LOCATION LG~3.A L.Q1.3 g~L{ S~:l-3 g~-1-& g51,9 Mdl-115 Mi<ITBS M\ll-re,N ,\--B~-:lPF~-16 - -~ UV { U'...1- CC..'&lvJ. MC,. 4114 NO. OF CON T A I NERS 1. 1 1 1 i 1- 1. 1- 1 1- 'J_ 1. ~I_/; ~o/ef; / V ./ / ./ ,/ / / / ./ ./ ,. I ;V V I REMARKS Sp.l1'ni'fY: (!).1. f PC (;~ 2(), C!l ,~ ~-3 6). 1- <9 d ct, - '2c,,_s (l') '6 a,, Z- JA--t'k.it" l '2 > l/ 5 (p 1- c:, I 0 I / (37 '-'2. 12-- Wit?::t:, t,unqr ~~, Rel in q u ~ (SignrurJ OiT~Date /Time Received by: rs,,,,.ru,.J :J-/,i.,/z.1 1 ,,,,~ . Received by: rs,,,,.ru,.J - Date {Time ~- r- "fSlfrno/~by, 0 Relinquished by: (Si,nru,-J Relinquished by: fSi,neru,-J Date /Time Remarks :tblf/g, I,y,s- Distribution: Original Shipmtnt ;-Copy t o Coordinator Fiald Flies Page 4 of 4 Date /Time I Dote t m Received by : (SignarurJ Received by : (Si,nrureJ c_ I. ,,,L.. C 1- 20 568 City of Worcester, MA (MAS010002) EPA MS4 Compliance Sampling Inspection Attachment C Laboratory Report for Enterococcus Analysis Author: LSASD Laboratory Services Branch Pages: 4 Page 26 of 26 Sampling Inspection Date: July 14, 2021 August 09, 2021 Michelle Coombs - LFSB US EPA New England R1 United States Environmental Protection Agency Laboratory Services and Applied Science Division 11 Technology Drive North Chelmsford, MA 01863-2431 Microbiology Laboratory Report Project Number: 21070010 Project: Worcester, MA MS4 Analysis: Enterococcus in Water Analyst: Jack Paar Date Samples Received by the Laboratory: 07/14/2021 Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in. Sample preparation and analysis was done following the EPA Region I SOP, ECA Enterolert3. Sample preparation and analysis was done following the EPA Region I method: Enterococcus by Defined Substrate Data were reviewed in accordance with the internal verification procedures described in the EPA New England OEME Biology QA Plan. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory. If you have any questions please call me at 617-918-8604. Sincerely, Jack A. Paar, III Biology Laboratory Manager Page 1 of 4 21070010ENTERO Water Microbiology Laboratory Data Qualifier Codes J = Estimate H = Exceeds holding time I = Exceeds incubation time At = Atypical overgrowth S = Lost sample V = Insufficient sample volume TNTC = Too numerous to count MB = Media blank +++ = Positive control - - - = Negative control SP = Spiked Sample L = Estimated, result below reporting limit (RL) ND = Not Detected, result less than RL D = Lab Duplicate P = Plate counts outside preferred range FDUP = Field Duplicate collect time not specified on Chain of Custody. Field duplicate LIMS default time = 12:00AM Page 2 of 4 21070010ENTERO Matrix: Stormwater UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 1 - NEW ENGLAND LABORATORY SERVICES BRANCH Worcester, MA MS4 Enterococcus in Water Sample Number LQ13A LQ13 BB4 BB13 BB10 BB19 MillTB MillTBS MillTBN AB07 PFB15 CCBlvd Lab ID Date of Collection AB92848 AB92849 AB92850 AB92851 AB92852 AB92853 AB92854 AB92855 AB92856 AB92857 AB92858 AB92859 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 8:40 am 8:50 am 9:20 am 9:55 am 9:50 am 10:15 am 10:42 am 10:50 am 10:56 am 11:30 am 11:55 am 12:20 pm Number of Samples: 12 Date of Analysis 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 07/14/21 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm 2:45 pm Compound Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Concentration MPN/100mL <10 241 213 813 620 426 171 <10 272 10 399 350 RL MPN/100mL 10 10 10 10 10 10 10 10 10 10 10 10 Qualifier Page 3 of 4 21070010ENTERO ~) ............... ........ ,r-. ,,.,.,.., ......,,n.,,,,.,,.,.._,.""'. REGION 1 CHAIN OF CUSTODY RECORD PROJ. NO. PROJECT NAME ')I 07oo /0 Wo,c.e.s\-er f, ,,r,r "- SAMP1/:C}J'~ .flv- ~I.f STA. NO. CD TIME 0 c<c u C) I rt- l'-1 b840 IX 7- ':f-, 14 ~o 3 i- lL{ OC\'Z.O X '-{ ".l-/ll{ oqs,s IX s 1/lt.l ~o 'x (,, 1/l4. \Ol5 ')( =t will lo'i'l. IX 2- 1/,l{ lOSo )( q 7-/I'{ loSCo r LO 1/M ll'30 D< l\ 7/l'{ llS.S I>< -- \. / ~J-' / .... \L t/\4 \'2.'U) I SL.\- lNon.:e~r,MA STATION LOCATION LG~3.A L.Q1.3 g~L{ S~:l-3 g~-1-& g51,9 Mdl-115 Mi<ITBS M\ll-re,N ,\--B~-:lPF~-16 - -~ UV { U'...1- CC..'&lvJ. MC,. 4114 NO. OF CON T A I NERS 1. 1 1 1 i 1- 1. 1- 1 1- 'J_ 1. ~I_/; ~o/ef; / V ./ / ./ ,/ / / / ./ ./ ,. I ;V V I REMARKS Sp.l1'ni'fY: (!).1. f PC (;~ 2(), C!l ,~ ~-3 6). 1- <9 d ct, - '2c,,_s (l') '6 a,, Z- JA--t'k.it" l '2 > l/ 5 (p 1- c:, I 0 I / (37 '-'2. 12-- Wit?::t:, t,unqr ~~, Rel in q u ~ (SignrurJ OiT~Date /Time Received by: rs,,,,.ru,.J :J-/,i.,/z.1 1 ,,,,~ . Received by: rs,,,,.ru,.J - Date {Time ~- r- "fSlfrno/~by, 0 Relinquished by: (Si,nru,-J Relinquished by: fSi,neru,-J Date /Time Remarks :tblf/g, I,y,s- Distribution: Original Shipmtnt ;-Copy t o Coordinator Fiald Flies Page 4 of 4 Date /Time I Dote t m Received by : (SignarurJ Received by : (Si,nrureJ c_ I. ,,,L.. C 1- 20 568