Document jNDvn2odrEBeZenryKMZnmBKp

"1 j- -v 1 1 1 CHRONIC TOXICITY OF EFFLUENT 1 FROH VISTA CHEMICAL COMPANY, VCM PLANT 1 TO THE FATHEAD MINNOW {Pimephales promelas) IN A STATIC RENEWAL BIOASSAY EN89305 1 Performed for: 1 Vista Chemical Company Attn: Paul Fetzer VCM Plant Road 1 Westlake, LA 70669 1 on September 25 - October 2, 1989 I i Performed by: J ENSR Consulting and Engineering (formerly ERT) Environmental Laboratory J 2400 West Loop South Suite 300 Houston, TX 77027 J J j J VVV 000004071 Formerly ERT CHRONIC TOXICITY OF EFFLUENT FROM VISTA CHEMICAL COMPANY, VCM PLANT ENSR Consulting and Engineering 2400 W est Loop South Suite 300 Houston. TX 7T027 (713) 960-8557 TO THE FATHEAD MINNOW (Pimephales promelas) IN A STATIC RENEWAL BIOASSAY EN89305 Performed for: Vista Chemical Company Attn: Paul Fetzer VCM Plant Road Westlake, LA 70669 on September 25 - October 2, 1989 Performed by: ENSR Consulting and Engineering (formerly ERT) Environmental Laboratory 2400 West Loop South Suite 300 Houston, TX 77027 REPORT FORMAT Version 3.2 July, 1989 000407.2 1 1 1. EXECUTIVE SUMMARY 1 1.1 OBJECTIVE: The objective of this study was to determine the chronic toxicity to Pimephales promelas of an effluent submitted by Vista Chemical Company. 1 1.2 SAMPLE DATES: The composite samples were collected by Vista Chemical Company personnel and received at ENSR 1 Consulting and Engineering Environmental Toxicology Lab on 9/25/89. 1.3 TEST DATES: A 7-day static renewal test was conducted 1 September 25 - October 2, 1989. 1.4 TEST PROTOCOL: The test was conducted according to methods specified by the U. S. Environmental Protection Agency (1985b, 1989), Method 1000.0. 1 1.5 TEST CONDITIONS: The test was conducted at a temperature of 251*C and a light intensity of 50 to 100 foot candles with a photoperiod of 16-hr light and 8-hr dark. 7 dilutions were tested: 26%, 52%, 59%, 67%, 1 76%, 86% and 100%. 76% is the critical concentration for pass/fail determination. 1.6 STANDARD TOXICANT: 24-Hour LC50 = 9.2 parts per thousand (ppt) I Sodium Chloride (NaCl). 95% Confidence Limits = 8-11 ppt. 1.7 DILUTION WATER: Receiving Water (Bayou Verdine) I 1.8 DATA ANALYSIS: This set of data could not be analyzed becausee of 100% control mortality. I 1.9 TEST RESULTS-LC50: An LC50 could not be calculated from this set of data J 1.10 TEST ACCEPTABILITY: Control 1 survival, 0%, does not meet minimum 80% survival. J 1.11 CONCLUSIONS AND COMMENTS: Laboratory water control survival (88%) and growth (0.18 mg/fish) demonstrated the fish used J in this test were suitable for toxicity testing. J J J VISTA CHEMICAL COMPANY -l- EN89305 VVV 000004073 1 1 2. INTRODUCTION The objective of this study was to determine by a 7-day static renewal test 1 the chronic toxicity to fathead minnow (Pimephales proroe7as) larval survival and growth of an effluent. Samples were collected by the permittee and shipped to ENSR Consulting and Engineering Environmental 1 Toxicology Lab - Houston, Texas. The 7-day chronic toxicity test was performed September 25 - October 2, 1989 in the Environmental Toxicology Laboratory at the ENSR Environmental Lab. This report follows the recommended outline by EPA (1985b, 1989) for chronic toxicity reports. All 1 data, laboratory notebooks, and associated documentation are archived at ENSR Environmental Toxicology Lab for future reference. 1 3. FACILITIES AND STAFF 1 All toxicity testing at the ENSR Environmental Lab was performed in the Environmental Toxicology Laboratory (approximately 3,000 sq. ft.). Test organisms were cultured or acclimated to test conditions in the Aquatic 1 Culture Facility (1,000 sq ft) separate from the Environmental Toxicology Laboratory. Both labs are located in the basement of the ENSR Consulting and Engineering building, constructed of 8" thick concrete walls and designed to minimize the effects of changing ambient climatic conditions. 1 All chemical tests were performed in the Chemistry Laboratory (500 sq ft) located on the fourth floor of the same building or at ENSR's Chemistry Laboratory (2925 Richmond Avenue, Houston, TX 77098). All areas of the I ENSR Environmental Toxicology Lab have separate, dedicated environmental control systems to avoid any possible air or water cross-contamination between facilities. A Sample Receiving Center (SRC) is located adjacent to the building's loading dock. It includes numerous laboratory refrigerators I and freezers for sample storage at 41'C. All samples were assigned a unique, coded number upon receipt and the progress of the sample was tracked on a computer sample tracking system (MASS-11) designed for ENSR I Environmental Lab. The Environmental Laboratory is supplied with fresh water for toxicity I testing from a 1,000 gallon freshwater system. Fresh water is aerated and conditioned prior to use and is filtered through a series of physical (5 micron), chemical, and biological filters, then oxygenated. Water is chemically analyzed quarterly for various inorganic and organic parameters. 1 Two high capacity Millipore Deionized water systems provide high quality makeup water. A Millipore Q Distilled Water system provides distilled water of even higher quality water for phytoplankton cultures or chemical I analyses. All biological testing was performed in a Nor-Lake Scientific (2'x6.5') or J two Bally self-contained Environmental Chambers (12'xl8' and 8'xlO') with dedicated microprocessor control systems capable of maintaining J J J VISTA CHEMICAL COMPANY -I- EN89305 VVV 000004074 1 1 temperatures within 1.0#C in a temperature range of 5-30*C. Temperature is displayed digitally and recorded on 7-day Partlow recording thermometers for documentation. A hi/low temperature alarm system is built in. 1 Lighting is provided by cool-white vaporproof fluorescent lights at an intensity of 70-foot candles. Light and dark photoperiods are maintained by Dayton automatic timers. Air is supplied by an oil-less Rotron Air 1 Blower. An emergency backup power generator maintains the supply of air during power shutdowns. 1 Test species raised in the culture facility have been taxonomically verified and voucher specimens are maintained. Where possible, the original source of the test organisms was from EPA stocks. Genetically characterized strains are maintained for various species. All culture 1 stocks are tested monthly and quarterly with a standard reference toxicant for the development of a cumulative database through successive generations. 1 Culturing of test organisms and the performance of all toxicity testing is carried out by a staff of degreed biologists. Biological and chemical 1 analyses are performed using appropriate EPA protocols. Quality control and quality assurance audits are performed by the QA/QC Officer of the Environmental Laboratory. 4. METHODS AND MATERIALS The type of plant, operating schedule, final products, schematic of wastetreatment, discharge information, flows, etc. have been previously i submitted to the EPA by the permittee in EPA Form 2C. Composite samples of the effluent were collected by the permittee, immediately placed on ice in a cooler and transported to Houston (Appendix i A). Chain-of-custody procedures were observed and the completed documents are attached (Appendix B). Effluent samples were stored at 4*C. Dilution water was receiving water. Appendix D describes the chemical characteristics of the effluent and receiving water. Testing was carried out according to procedures specified in permittee's i permit and Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms (EPA, 1985b, 1989). j Test organisms were fathead minnow (Pimephales promelas) larvae less than 24-48 hrs old, as per the EPA freshwater chronic manual (1989). The larvae were exposed in a static renewal test for 7 days to 7 different effluent j concentrations 26%, 52%, 59%, 67%, 76%, 86% and 100% and 2 controls. Control 1 (dilution water) was receiving water (Bayou Verdine) and Control 2 was moderately hard lab water. j j j VISTA CHEMICAL COMPANY -2- EN89305 VVV 000004075 1 1 Renewal solutions (effluent concentrations) were prepared the day that each composite sample arrived at ENSR Environmental Toxicology Lab. A sufficient 1 quantity of each test solution was prepared for three days of testing (i.e., three consecutive renewal periods). The reservoirs of test solution were stored at 4*C. 1 All glassware was cleaned according to procedures specified in Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms (EPA, 1985b, 1989) and described in detail 1 in "ENSR Consulting and Engineering, ENSR Environmental Laboratory Standard Operating Procedures Manual. (September 1988; revised May, 1989). The test chambers were Nitex cylinders placed inside 1-liter beakers. The 1 Nitex cylinders were constructed by attaching a 7-cm high collar of 363micrometer mesh Nitex screen to a 15x60 mm glass Petri dish with silicone sealant. Larvae were placed into the cylinders in random order until 10 1 larvae were in each cylinder. The test was initiated by randomly placing a Nitex cylinder containing 10 1 larvae into the test chamber. A total of 40 larvae was tested for each concentration (4 replicates per concentration). The light quality and intensity were at ambient laboratory levels of 50 to 100 foot candles, with a photoperiod of 16-hour light and 8-hour dark (16L:8D). The water temperature in each test chamber was maintained at 251 *C. The larvae in each test chamber were fed newly hatched (<24-hr old) brine shrimp nauplii three times daily: at the beginning, midway and at the end of each day. The nauplii were soaked in fatty acid, vitamin and trace element solution to enhance their nutritional value. The nauplii were rinsed with moderately hard lab water just prior to feeding. The number of live larvae in each test chamber were recorded daily and the dead larvae discarded. Just prior to the daily renewal of the test I solutions, uneaten and dead brine shrimp and other debris were carefully removed from the bottom of each Nitex cylinder with a new polyethylene dropper. I Test solutions were renewed by gently lifting the Nitex cylinders out of the test chambers and placing them into new test chambers (1-1 iter beakers) i containing new test solutions (250 ml). During the transfer, the larvae remained in the solution filled petri dishes at the bottom of the Nitex cylinders. J This procedure for the daily renewal of the test solutions was observed to be far less stressful to the larvae than the recommended siphon technique (Method 1000.0; EPA, 1985b) which often leads to the inadvertent removal of J larvae (i.e. caught up in the siphon). The test solution volume was also reduced from the recommended 500 mL (EPA 1000.0) to 250 mL per replicate to reduce the volume of sample required for J the test. This alternative is consistent with the testing protocol used by EPA Region VI Toxicity Testing Laboratory, Houston, Texas and the second edition of the EPA manual (1989). J I VISTA CHEMICAL COMPANY -3- EN89305 WV 000004076 1 1 Oissolved oxygen was measured at the beginning and end of each 24-hr exposure period at all test concentrations and in the control. Temperature, pH, and conductivity were measured at the beginning of each 1 24-hr exposure period at all test concentrations and in the controls. This information is contained in Appendix D. 1 The test was terminated after seven days of exposure. At termination, the live larvae in each test chamber were counted and preserved as a group in 4% formalin or 70% ethanol. For the dry weight analysis, the preserved 1 larvae from each test chamber were rinsed in deionized water, transferred to tared weighing boats and dried at 100*C for a minimum of 2-hrs. Immediately upon removal from the drying oven, the weighing boats were placed in a dessicator. Weights were measured to the nearest 0.10 mg. 1 All data were analyzed according to the statistical flow chart outlined in the second edition of the EPA Freshwater Chronic Methods Manual (1989). 1 Figure 1 is a generic flow chart of the statistical approach used in this report. Statistical analyses are included in Appendix E. 1 Survival and growth data from the fathead minnow larval survival and growth test are analyzed using hypothesis testing techniques. TOXSTAT, TOXDAT and a proprietary computer program are used for all calculations. Survival data are arc-sine transformed and both data sets are tested for normality by Shapiro-Wilk's test. Normal data are then tested for homogeneity of variances by Bartlett's test. Data passing both tests are analyzed by Dunnett's test (Dunnett, 1955). Dunnett's test is a two stage test and consists of an analysis-of-variance (ANOVA) to determine if treatment means are different from each other. If treatment means are significantly different, then the second test, a post-hoc multiple-range comparison is made between the treatment means and the control mean. If treatment means I are equal, the post-hoc comparison is not performed (Snedecor and Cochran, 1980). I Data that do not meet the normality assumptions are analyzed by a nonparametric test, Steel's Many-One Rank test. If an unequal number of replicates exist, Wilcoxon Rank Sum test with Bonferroni's Adjustment is ] used alternatively. A modified version of the TOXDAT program is used to calculate an LC50 if mortality occurred in the higher test concentrations. The LC50 is ] calculated by three different statistical models (binomial, moving-average, and probit) and the model fitting the data best is used. LC50's are corrected for control mortality by a maximum likelihood estimation method. J The LC50 is reported as an unadjusted value. J 5. RESULTS - Effluent The results derived from the 7-day static renewal test with the fathead minnow (Pimepbales promelas) larvae and effluent are shown in Table 1. J Table 2 lists the percent survival in replicate chambers and the mean percent survival at 24-hr (Day 1), 48-hr (Day 2) and 7-days. The test results were not acceptable with survival in the controls less than 80% J (EPA, 1985b, 1989). Table 3 is a summary of the growth data. J VISTA CHEMICAL COMPANY -4- EN89305 VVV 0000040 77 Table 1. Survivorship during the 7-day static renewal test. Cone.(%) 0 Test Day FProportion 1 2 3 4 5 6 7 Surviving 0% A 10 0 0 0 0 0 0 0 0.00 CONTROL 1 B 10 0 0 0 0 0 0 0 0.00 C 10 0 0 0 0 0 0 0 0.00 D 10 0 0 0 0 0 0 0 0.00 26 % A 10 0 0 0 0 0 0 0 0.00 B 10 0 0 0 0 0 0 0 0.00 C 10 0 0 0 0 0 0 0 0.00 D 10 0 0 0 0 0 0 0 0.00 52 % A 10 0 0 0 0 0 0 0 0.00 B 10 0 0 0 0 0 0 0 0.00 C 10 0 0 0 0 0 0 0 0.00 D 10 0 0 0 0 0 0 0 0.00 59 % A 10 0 0 0 0 0 0 0 0.00 B 10 0 0 0 0 0 0 0 0.00 C 10 0 0 0 0 0 0 0 0.00 D 10 0 0 0 0 0 0 0 0.00 67 % A 10 00 0 0 0 0 0 0.00 B 10 0 0 0 0 0 0 0 0.00 C 10 0 0 0 0 0 0 0 0.00 D 10 0 0 0 0 0 0 0 0.00 76 % A 10 0 0 0 0 0 0 0 0.00 B 10 0 0 0 0 0 0 0 0.00 C 10 0 0 0 0 0 0 0 0.00 D 10 0 0 0 0 0 0 0 0.00 86 % A 10 0 0 0 0 0 0 0 0.00 B 10 0 0 0 0 0 0 0 0.00 C 10 0 0 0 0 0 0 0 0.00 D 10 0 0 0 0 0 0 0 0.00 100 % A 10 0 0 0 0 0 0 0 0.00 B 10 0 0 0 0 0 0 0 0.00 C 10 0 0 0 0 0 0 0 0.00 D 10 0 0 0 0 0 0 0 0.00 CONTROL 2 A 10 10 10 10 10 9 9 8 0.80 B 10 10 10 10 9 9 9 9 0.90 C 10 10 9 9 9 8 8 8 0.80 D 10 10 10 10 10 10 10 10 1.00 VISTA CHEMICAL COMPANY -5- EN89305 Wv 000G4078 1 1 FATHEAD MINNOW 1 Table 2. Summary of survival data of fathead minnow larvae exposed for 7 days to the effluent. 1 Cone.(%) Replicate ABC Time D 24 H 48 H 7 D CV* 1 0 % 0.00 0.00 0.00 0.00 0.00 0.00 0.00 26 % 0.00 0.00 0.00 0.00 0.00 0.00 0.00 52 % 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1 59 % 0.00 0.00 0.00 0.00 0.00 0.00 0.00 67 % 0.00 0.00 0.00 0.00 0.00 0.00 0.00 76 % 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1 86 % 100 % 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1 CONTROL 2 0.80 0.90 0.80 1.00 1.00 0.98 0.88 9 * CV = StDev*100/Mean 1 I e 3. Summary of growth data of fathead minnow larvae exposed for 7 days to the effluent. I Replicate Statistics Conc.(%) A B C D Mean StDev i:v% I I CONTROL 2 0.19 0.19 0.20 0.15 0.18 0.02 10 ] The survival data were analyzed with the EPA TOXDAT Program. TOXDAT could not calculate an LCs0 due to 100% control mortality. Survival and growth data were not analyzed because of dilution water (receiving water) toxicity. The dilution water control showed 100% mortality in 24 hours. J J J J J VISTA CHEMICAL COMPANY -6- EN89305 VVV 000004079 6615001 FIGURE 1 FLOW CHART FOR STATISTICAL ANALYSIS OF CHRONIC TOXICITY TEST DATA MODIFIED FROM EPA (1988, 1989) VVV 000004080 1 1 6. DISCUSSION 1 The response of a subsample of the fathead minnow larvae population used in this toxicity test to a reference (standard) toxicant (NaCl)f as well as survivorship in the laboratory water control showed that the test organisms were in suitable condition for use in toxicity testing. ENSR Environmental 1 Toxicology Lab routinely runs bioassays with a reference toxicant to determine the sensitivity of test organisms. When compared with historical standard toxicant results, these data indicate the similarity in 1 sensitivities of test organism populations at different times and thus the extent to which tests performed with the same species at different times may be considered comparable. All requirements of the prescribed protocol (EPA, 1985a) were adhered to and conditions were maintained within the 1 required limits throughout the test. 1 1 i i i j 1. NOEL (or NOEC). No observed Effect Level (or Concentration) - the highest concentration of toxicant j (effluent) to which the organisms are exposed in a full life-cycle or partial life-cycle test, which causes no statistically significant adverse effect on the observed parameters (EPA, 1985b) j j VISTA CHEMICAL COMPANY -8- EN89305 000G408I 1 1 7. LITERATURE CITED 1 Dunnett, C.W. 1955. Multiple comparison procedure for comparing several treatments with a control. J. Amer. Stat. Assoc. 50:1096-1112. EPA, 1985a. Methods for measuring the acute toxicity of effluents to 1 freshwater and marine organisms. EPA/600/4-85/013. Environmental Monitoring and Support Lab. Cincinnati, Ohio. Third Edition. 216 pp. 1 EPA, 1985b. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. EPA/600/485/014. Environmental Monitoring and Support Lab. Cincinnati, Ohio. 1 162 pp. EPA, 1988. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to marine and estuarine organisms. 1 Environmental Monitoring and Support Lab. Cincinnati, OH. EPA 600/4-88028. 417 pp. 1 EPA, 1989. Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. Environmental Monitoring and Support Laboratory. Cincinnati, OH. EPA 600/4-89-001. February, 1989. 250 pp. ] Snedecor, G.W., and W.G. Cochran. 1980. Statistical Methods. Seventh Edition. Iowa State University Press. Ames. 593 pp. i i i i j j j j VISTA CHEMICAL COMPANY J -9- EN89305 vvv 000004082 8. QUALITY ASSURANCE/QUALITY CONTROL CHECKLIST AND CERTIFICATION ENSR CONSULTING AND ENGINEERING ENVIRONMENTAL LABORATORY - HOUSTON QUALITY ASSURANCE / QUALITY CONTROL PROCEDURES CHECKLIST FATHEAD MINNOW LARVAL SURVIVAL AND GROWTH TEST METHOD 1000 (EPA-600/4-85/014, FEB 1989) (Section numbers in parentheses refer to method section(s) where requirement is discussed.) A. Sample Characteristics Yes No 1. Sample quality maintained according to protocol requirements (8.0)? 2. Sample tested within time period required by protocol (8.3.1)? 3. Salinity measured to determine if freshwater (5 ppt) test required? B. Testing Conditions 1. Standard toxicant LC50 is reasonable? 2. <24-48 hr old larvae acclimated (if necessary) 3. Test temperature maintained at 251C? 4. Dissolved oxygen concentration 40% saturation? 5. Aeration required? 6. Protocol followed without deviation? C. Test Acceptability Criteria 1. Laboratory water controls a. Average survival >80%? b. Average dry weight of preserved larvae >0.25 mg/fish? 2. Receiving water controls a. Average survival >80%? b. Average dry weight of preserved larvae >0.25 mg/fish? 3. Coefficient of variation between replicates <40% for survival? 4. Coefficient of variation between replicates <40% for growth? M D. Report 1. Report pages are numbered in sequence? 2. Each page contains the sample number? 3. Page numbers listed in the table of contents correspond to pages in the body of the report? VISTA CHEMICAL COMPANY -10- EN89305 VVV 000004083 1 1 4. Headings listed in the Table of Contents correspond to headings in the body of the 1 report? 5. Titles of tables listed in the Table of Contents correspond to tables in the body of the report? 1 6. Titles of figures listed in the Table of Contents correspond to figures in the body of the report? 1 7. Values appearing more than once in the text agree? 8. Tables and figures appear in the order in 1 which they are referenced in the report? 9. Horizontal tables and figures are bound above the top edge? 10. Sample conditions upon receipt adequately reported? 11. Explanations are provided for all footnoted items? 1 12. Units are provided for all columns and rows of data? 13. Appendices attached to report? A. Sample Receiving Forms 1 B. Chain-of-Custody C. Chemical analysis of tap water, deionized water and saltwater used in ENSR 1 Environmental Laboratory - Houston D. Initial characterization of effluent and receiving water I E. Water Quality Parameters Lab Notebook F. Statistical Analyses of Data I E. COMMENTS I j ] J J J J VISTA CHEMICAL COMPANY -11- EN89305 VVV 000004084 1 1 TABLE OF CONTENTS 1 SECTION 1. EXECUTIVE SUMMARY PAGE i 1 2. INTRODUCTION 3* FACILITIES AND STAFF 1 4* METHODS & MATERIALS 1 1 2 5. RESULTS 4 1 Table 1. Survivorship during the 7-day static renewal test. 5 1 Table 2. Summary of survival data of fathead minnow larvae exposed for 7 days to theeffluent. 6 1 Table 3. Summary of growth data of fathead minnow larvae exposed for 7 days to theeffluent. 6 I Figure 1. Flow chart for statistical analysis of chronic toxicity test data. From EPA (1985b; 1988:30; 1989:9). 7 6. DISCUSSION 8 7. LITERATURE CITED 9 8. QUALITY ASSURANCE/QUALITY CONTROL CHECKLIST ANDCERTIFICATION 10 I 9. APPENDICES A. Sample Receiving Form I B. Chain-of-Custody C. Chemical Analysis of Tap Water, Deionized Water, and Saltwater Used in ENSR Environmental J Toxicology Lab - Houston D. Initial Characterization of Effluent and Receiving Water E. Water Quality Parameters Lab Notebook I J J J J VVV 000004095 1 1 CERTIFICATION Data presented in this report were derived by methods and materials 1 identified in the section of the report entitled "Methods and Materials," in accordance with the protocol specified by the U.S. Environmental Protection Agency {1985b, 1989). All laboratory practices and procedures 1 were in accordance with ENSR Environmental Toxicology Lab Standard Operating Procedure and the ENSR Environmental Toxicology Lab Standard Operating Procedures Manual, {September 1988, May 1989). 1 A project notebook was maintained during the testing program, and all data transcribed from the notebook to the report were checked for accuracy by two people. 1 1 1 I I I I I i HJ/mr J 23-0CT-89 QA/QC Officer, ENSR Environmental Toxicology Lab J J j VISTA CHEMICAL COMPANY -12- EN89305 0004086 ' 1*- . *.- 1 1 1 1 1 1 --w. *; 1 APPENDIX A 1 Sample Receiving Form I I I I I J J J J J 00oo^o? * ' - " '***&&* SAMPLE CHECK-IN FORM PROJECT Is EN # : DATE : SALINITY : DO : pH TEMP *C : CONDUCTIVITY BLACK SPOTS OUL ODOR <Zb\S-&)Z-d3cj gq.3 oS.nl ikS { C7 9.5- 7.6 n.o C, ooo M-'-rfs" E4^YES Ll^YES [] YES [] YES SAMPLE CONTAINER [] POLY [] GLASS [] 1 gallon Letica pail [] 500 ml [3 1000 ml H-oTher z.S E3 OTHER ENSR KIT: [il^es [3 no If not, describe: [] Igloo Cooler [] styrofoam cooler [] IGLOO ICE: [] frozen [^ool [3 no Igloo ice [] warm RETORT % (Vvol): [] yes __________ % water __________ % oil ____ ____ % solids NITROGEN: [] yes [] NO [] NO [] NO [] NO y.NO H-NG"- (juL*. OQCOCKoaa V-"- ' "j OROJECT #! "| EN # 1 DATE 1 SALINITY : DO 1 pH 1 TEMP *C SAMPLE CHECK-IN FORM m. -037 5" 5" Hi P^ES [] NO H--YES' [] NO 61^S^ f] NO CONTAINER [] GLASS [] 500 ml [] 1000 ml [] OTHER ENSR KIT: [<f-?es^ [] no If not, describe: [] Igloo Cooler [] styrofoam cooler [] IGLOO ICE: [] frozen [<pool [] warm [] no Igloo ice j RETORT % (voi/vol): [] yes j % water tJ-mr-- % oil j NITROGEN: [] yes LLna-"-" % sol ids VVV 00000A089 APPENDIX B Chain-of-Custody VVV 000004090  Lake Charles Chemical Plant Chaln-of-Custody Record drpy) k. ^/irnfcfrjf/crY/'ij PROJECT NAME/NUMBER SAMPLE TEAM MEMBERS - C&fKr**- - " >wlft Chuiniuill Compwy * r Kies VCM Plant 0 Lake Charles LAB Plant \ R/A Control No. C/C Control N2 _01460' LAB DESTINATION CARRIER/WAYBILL NO.. Sample Number / C? 3 V OOl co ! Sample Location and Description Out11 otuSf _________________ <96 / &*?&// rxirrMi Date and Time Collected Sample Type Container Type Condition on Receipt (Name and Date) Disposal Destination VVV 000004091 Special Instructions: Possible Sample Hazards:. SIGNATURES: (Name, Company, Date and Time) 1. Relinquished By 3. Relinquished ry \06 q-otf-m Received By: 2. Relinquished By: Received V8-M-X1 Received B 9-^y-y? 9-4*#? Sits' 4. Relinquished By: 3^' Received 9-a*-`* White To Accompany Samples Canary Laboratory Copy The white copy Is to be returned to the Environmental Coordinator at the plant of orlolnetlon after the enalvele Is completed. m-u. Chain-of-Custody Record PROJECT NAME/NUMBER Cl+-*nit. j^) l* SAMPLE TEAM MEMBERS Sample Number 3" Sample Location and Description 0,01 CC '9-/9^ 50/ Cuf&cj Date and Time Collected LAB DESTINATION CARRIER/WAYBILL NO.. Sample Type Container Type C/C Control N2 _01461 Condition on Receipt (Name and Date) Disposal Destination 70 6 ~7b o?<B ---------- f------- ---------g-------------- -tf------------ $/<L A o/'/Co Special Instructions: Possible Sample Hazards: SIGNATURES: (Name, Company, Date and Time) 1. Relinquished By: fee**Bv: '-pLu^sjfr 9- 2 S- _______oj^> &un 2. Relinquished By: Received BC jj&UJ i4 s/- 9-*-rvf7 a fa) j___ O f.O^ Received By:. Whit* - To Accompany Samples Canary - Laboratory Copy The white copy is to be relumed to the Environmental Coordinator at the plant of origination after the analysis la complstad. pjnk Sample Teem Copy VVV 000004092  Lake Charles Chemical Plant CIlciflwH Corfipw^ jiles VCM Plant Lake Charles LAB Plant Chaln-ol-Custady Record ckrpy\ i c B>ianv?y\ / PROJECT NAME/NUMBER an r\ SAMPLE TEAM MEMBERS LAB DESTINATION CARflIER/WAYBILL NO. Sample Number 1 Sample Location and Description BcuiryiA 0 h~h&r- o Date and Time Collected ifisfa- om Sample Type Container Type plasfi cl R/A Control No. C/C Control N2 .01463' Condition on Receipt (Name and Date) Disposal Destination FAAsf vfV ,, Special Instructions:_____ Possible Sample Hazards: SIGNATURES: (Name. Company, Date and Time) 1. Relinquished Bv:*a^___V /__\ < < < Received -------- -----Q -f -- The white cnnv Is t>> h* *^-^V__ o `jazJ ? O O 9 -2. jTs/9 Received By: White - To Accompany Sample* Canary Laboratory Copy the Environmental Coordinator el the olent nf orHfnetlon after the snelvsls Is completed. Pink - Snmole Tnam Copv 00C004093 APPENDIX C Chemical Analysis of Tap Water, Deionized Water, and Saltwater Used in ENSR Environmental Toxicology Lab - Houston VVV GG0004094 Chemical analysis of tap water, deionized water, and saltwater used in ENSR Environmental Laboratory. Specific analytical references, detection limits and QVQC results are available upon request. N.D. - Not Detected and N.M. - Not Measured. ____________PARAMETER_______ _______ IMIS___ Inorganic Parameters A1kalinlty Hardness TDS TSS Ammonia mg/L mg/L mg/L mg/L mg/L TCP Metals/Elements A1uminum Cadmium Hexavalent Chromium Copper Iron Lead Manganese Mercury Selenium Zinc m/l pg/L mg/L pg/L pg/L Mg/L /*g/L Mg/L Mg/L Mg/L 0RGAN0CHL0R1NE. Pesticides ALPHA-BHC BETA-BHC DELTA-BHC GAMMA-BHC (LINDANE) HEPTACHLOR ALDRIN HEPTACHLOR EPOXIDE ENDOSULFAN I DIELDRIN 4,4'-DDE ENDRIN ENDOSULFAN II 4,4.'-DDD ENDRIN ALDEHYDE ENDOSULFAN SULFATE 4,4'-DDT ENDRIN KETONE METHOXYCHLOR TOXAPHENE AROCLOR-1016 AROCLOR-1221 AROCLOR-1232 AROCLOR-1242 AR0CL0R-1248 AR0CL0R-1254 AROCLOR-1260 md/l Mg/L mq/l Mg/L Mg/L Mg/L Mg/L Mg/L Mg/L md/l md/l Mg/L Mg/L Mg/L Mg/L md/l md/l md/l md/l Mg/L Mg/L Mg/L md/l Mg/L md/l Mg/L Tap Water Deionized Saltwater Water (25 Q/oo) 210 100 280 N.D. 1.1 4 N.D. N.D. N.D. N.M. 62 4000 20,000 4 N.M. N.D. N.D. N.D. .04 0.4 N.D. 4.4 N.D. N.D. N.D. N.M. N.M. N.M. N.D. 0.08 N.M. N.M. N.M. N.M. 0.3 N.D. N.D. N.D. .03 0.06 53.4 N.D. N.D. N.D. N.D. N.D N.D N.D. N.D. N.D. N.D N.D N.D N.D. N.D. N.D. N.D N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. Wv 000004095 1 Tap Deionized Saltwater ____________parameter____________ Units Hater Water f25 /oo1 ] Oraanic Parameters. Volatile 1 Chloromethane Bromomethane Vinyl Chloride Chloroethane * 1 Methylene Chloride 1,1-Diehloroethane /xg/L /xg/L /xg/L /xg/L /xg/L /xg/L N.D. N.D. N.D. * N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.M. N.M. N.M. N.M. N.M. N.M. 1,1-Dichloroethane /xg/L N.D. N.D. N.M. 1 1,2-Dichloroethene (total) Chloroform ftg/L fig/i N.D. N.D. N.D. N.D. N.M. N.M. 12-Dichioroethane /xg/L N.D. N.D. N.M. 1 1 * 1,1-Trlchioroethane Carbon Tetrachloride /Xg/L #*g/L N.D. N.D. N.D. N.D. N.M. N.M. Bromod1 chioromethane //g/L N.D. N.D. N.M. 1,2-D1chioropropane /*g/L N.D. N.D. N.M. 1 Cis-1,3-Dichioropropene g/L N.D. N.D. N.M. Trichioroethene //g/L N.D. N.D. N.M. Dibromochloromethane /xg/L N.D. N.D. N.M. 1 1,1,2-Trichioroethane Benzene /xg/L M/L N.D. N.D. N.D. N.D. N.M. N.M. Trans-1,3,-Diehloropropene /xg/L N.D. N.D. N.M. 1 2-Chloroethylvinyl ether Bromoform /xg/L /xg/L N.D. N.D. N.D. N.D. N.M. N.M. Tetrachloroethene /xg/L N.D. N.D. N.M. 1,1,2,2-Tetrachloroethane MJ/L N.D. N.D. N.M. I Tolvene Chlorobenzene /xg/L /xg/L N.D. N.D. N.D. N.D. N.M. N.M. Ethylbenzene pg/L N.D. N.D. N.M. I Trichiorofluoromethane /xg/L N.D. N.D. N.M. Compound is present, but below the detection limit (10 /xg/L). I Organic Parameters: Pthalate Esters Dimethyl Phthalate /xg/L N.M. N.M. N.D. ] Diethlyl Phthalate DI-N-Butyl Phthalate /*g/L /xg/L N.M. N.M. N.M. N.M. N.D. N.D. Butyl Benzyl Phthalate M9/L N.M. N.M. N.D. J Bis(2-Ethyl hexylJPhthalate DI-N-Octyl Phthalate /xg/L A9/L N.M. N.M. N.M. N.M. N.D. N.D. Organic Parameters. Semivolat.ilR* (Mpt.hnri J Benzo(k) f1 uoranthene /xg/L N.D. N.D. N.D. bis(2-Chloroethoxy)methane ^g/L N.D. N.D. N.D. J bis(2-Chloroethyl)ether bis(2-Chloroisopropyl)ether /xg/L 1*9/L N.D. N.D. N.D. N.D. N.D. N.D. bis(2-Ethylhexyl)phthalate /xg/L N.D. N.D. N.D. J 4-Bromophenyl phenyl ether Butyl benzyl phthalate 2-Chloronaphthalene /xg/L /xg/L /xg/L N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 4-Chlorophenyl phenyl ether /xg/L N.D. N.D. N.D. J Chrysene /xg/L N.D. N.D. N.D. J Wv 000004096 JVZ&Z'f'l Tap Deionized Saltwater 1 PARAMETER Units Water Water (25 o/ool 1 D1benzo(a,h)anthracene 12-Dichlorobenzene M9/L jig/L N.D. N.D. N.D. N.D. N.D. N.D. 1,3-Dichlorobenzene /*g/L N.D. N.D. N.D. 1 1,4-Oichiorobenzene 3*3'-D1 chiorobenzldlne M/L m/l N.D. N.D. N.D. N.D. N.D. N.D. Diethyl phthalate M/L N.D. N.D. N.D. Dimethyl phthalate A9/L N.D. N.D. N.D. 1 Di-n-butyl phthalate 2,4-Dinitrotoluene 09/ L M9/L N.D. N.D. N.D. N.D. N.D. N.D. 2,6-Dinltrotoluene ms/l N.D. N.D. N.D. 1 Di-n-octyl phthalate 1,2-Diphenylhydrazine M9/L <*9/L N.D. N.D. N.O. N.D. N.D. N.D. Fluoranthene #g/L N.D. N.D. N.D. Fluorene 1 Hexachlorobenzene 09/L M9/L N.D. N.D. N.D. N.D. N.D. N.D. Hexachlorobutadiene pg/L N.D. N.D. N.D. Hexachlorocyclopentadiene ^g/L N.D. N.D. N.D. 1 Hexachloroethane Indeno(l,2,3-c,d)pyrene /ig/L M9/L N.D. N.D. N.D. N.D. N.D. N.D. Isophorone raA N.D. N.D. N.D. Naphthalene 1 Nitrobenzene M9/L 09/L N.D. N.D. N.D. N.D. N.D. N.D. N-Nitroso-dipropylamine /*g/L N.D. N.D. N.D. I N-Nitrosodiphenyl amine Phenanthrene Pyrene m/l /*g/L #tg/L N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 1,2,4-Trichiorobenzene /tg/L N.D. N.D. N.D. 1 2-Chlorophenol 2,4-Dichlorophenol ot/l Wl/L N.D. N.D. N.D. N.D. N.D. N.D. 2,4-Dimethylphenol w/l N.D. N.D. N.D. I 4,6-D1nitro-2-methylphenol 2,4-Dinitrophenol 2-Nitrophenol M9/L #fg/L M9/L N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. N.D. 4-Nitrophenol /tg/L N.D. N.D. N.D. I 4-Chloro-3-methylphenol Pentachlorophenol W/L /*g/L N.D. N.D. N.D. N.D. N.D. N.D. Phenol 0g/L N.D. N.D. N.D. I 2,4,6-Trichlorophenol <ig/t N.D. N.D. N.D. I I J J J VV^ 000004097 Si??** APPENDIX D Initial Characterization of Effluent and Receiving Water i j j j j VVV G0000409ti Initial characterization of effluent and receiving water upon receipt and prior to mixing test concentrations. Sample A1kali n i ty Hardness Conductivity Temp. pH D.O. nh3 (Date Rec'd) (mg/L as CaC03) (/imhos/cm) ro (units) (mg/L) (mg/L) Effluent* EN89305.01 (9/25/89) 161 Receivina Waters EN89305.02 (9/25/89) 162 2785 2573 6000 5500 13.0 7.6 9.5 0.04 12.2 7.4 2.8 0.04 ^Effluent is Vista Chemical Company, VCM Plant ^Receiving water is Bayou Verdine. VVV 000004099 ,***' * n,*5P3S*^-: ^-TT; * APPENDIX E Water Quality Parameters Lab Notebook VVj, 00^4i0o