Document jM7kmOgkM7LXz9R3RmMd6Yp

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:Perfluorooctanesulfonylamido(ethyl)acemtaaytea;lso be referredto as PFOSAA, FC-128 or as the major component of FC-1 27, FC-1 29, FC-1 09, FC-1 09-X or Rl 904. (Glycine,NethylN--[(heptadecafluorooctyul)Isfonyl]p-o,tassium salt,CAS 2991-51-7) Remarks: The testsample isFC-129 referredto by the testlaboratory as Rl 904. Currentinformationindicatesitisa mixtureof42% test substance,32% water,14% 2-butoxyethanol4,% ethanol,and 8% C4-C7 fluoroalkyclarboxylatecompounds. Test materialwas from 3M production lot930014, mfg. date 3/97. The followingsummary isabbreviated because the testsample isa mixture of the testsubstance in a waterlorganic solvent solution. Date may not accurately relatetoxicityof the testsample with thatof the testsubstance. METHOD: Methods: OECD 203 Test type:Staticacute GLP: No Year Completed: 1997 Species: Pimephales promelas Analyticalmonitoring:Temperature,pH, DO, and conductivity RESULTS Nominal concentrations: Bk control1,9,32, 54,90, 150,250 mg/L (in duplicate) Element values: 24-hourLC50 = >250 mg/L (C.I.not calculable) 48-hourLC50 = 211 mg/L (C.I.not calculable) 72-hourLC50 = 146 (128-167)mg/L 96-hourLC,o = 45 (22-80)mg/L 96-hourLC50 = 97 (81-116)mg/L 96-hour NOEC = 54 mg/L Allelement values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureofthetestsubstance as describedinthe testsubstance remarks field.The value reportedapplies to thatmixtureand notthe testsubstance. No attemptwas made to determinethe impact ofthe presence ofthe organicsolventsor what portionofthe toxicitcyan be contributetdothe Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC- 129 96-hour LC50 forPimephales promelas was determined to be 97 mg/L witha 95% confidenceintervaolf81-116 mg/L. The 96-hourno observed effectconcentrationwas 54 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcriterifaorquality testingb,ut lacksanalyticaclonfirmationoftestsubstance concentrations. There isa lackofcharacterizatioonfthe testsample. REFERENCES This study was conducted atAScl CorporationofDuluth,Minnesota atthe requestofthe 3M Company. OTHER Last changed: 5/17/00 ASelCorpontioim/AScI-Duhith EnvuoamcaW TesUnSDivuwn ASelStudyID# 50104N STUDY ACUTE T03UCITY OF R1904 TO FATHEAD MINNOW (Pintephakpsromelm) DATA STANDAM OECD GUIDELM 203 STUDY COMPLETED June 27, 1997 TESTING FACELITY ASCI Corpomtion/AScl-Duluth EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,BdN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY . IDENTIFICATION NUMBERS AScl Study ID# 5010-068 3M Company Lab Request#R1904 Sponsor 3M Caapwy Sponsor Study IDO R1904 AScl COrPomtion/ASel-Duluth EnvironmentalTestingDivision AScl Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor'sRepresentative Sponsor'sLab Request# TestingFacility PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitivTeest Dates Data Validationand Report Review Acute Toxicityof R1904 to FatheadMinnow (Pimephalespromelas). OECD Guideline203. 3M EnvironmentalTechnology and Safety Services,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133;Tel No. (612)778-7452. Susan Beach, 3M EnvironmentalTechnology and SafetyServices,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133; Tel No. (612)778-7452. R1904. ASCI Corporation/AScI-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811; Tel.No. (218)722-4040. Joe Dierkes Joe Amato Donald Mount June 17-21,1997. Alan Mozol R rtSignatur Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport Two yearsfrom thedatestudyiscompleted. ASCI Corporation/AScl-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811;Tel.No. (218)722-4040. Sponsor: 3M Company Sponsor Study ID# R1904 2 ASelCorporadon/AScI-Duluth EavitenumudTestinDgiviska ASclStudyMi 5010-M 2.0OBJECTIVE(S) TO.1determinethe 96-hOur 10% and 50% effectconcentration(sLCIO and LCSO, and the96-hour no ood=bbeservableeffea concentratio(nNOEC) of thetestsubstanceR,1904, forfatheadminnow (Pimp:ph7ha0kiy2s4pronwlas)under statitcestconditions. This studywas conductedaccordingto theAScl studyplanretainedby theSponsor and OECD GG(TuWuLrideline203 (OECD 1993). TestSubsta Test Organisms DilutionWater 3.0 TEST UMTHODS Propeniej:R1904, bright-yeuowliquids,pecifigcravity= 1.3 (water=1.0),stablea,nd completelysolublein water. Testconcentrations0: (dilutiownatercontrol)1,9,32, 54, 90, 150,and 250 mg/L. Testsolutionpsreparedby mass additionof testsubstanceto dilutiownaterfollowedby vigorousstirring. Source: Healthy,juvenilefatheadminnows (Pimephalespromelas) obtained from AScl-DEM in-houseculture. HoOnglacclimation: The fishused in thetestswere obtainedfrom AScl's in-houseculture.The fishused in thestudywere hatchedon May 24, 1997. For'the48 hoursprecedingtestinitiatimoonrtalitywas 0%, and the fishwere not fed for24 hoursbeforetestinitiationT.he fishmean length was 17.0 2.6 mm, and mean wet weightwas 39 23 mg. Loading rate was calculatedtobe 127 mg of fishper L testsolution. Use intest:Used 10 organisms/replicaatned 2 replicates/test concentration. Source: DechlorinatedCityof Duluth,MN, tapwater.The most recent chemicalanalysisisin Raw Data Package. CharacteristicsA:t testtime,the water had a hardnessof 48 mg/L (as CaCO3) and a pH of 8.03.Aeratedfor24 h priortouse inthetest. Sponsor:3M Company SponsorStudyM# R1904 3 ASetCorpomtion/AScl-Duluth EnvironmentTaelstinDgivision ASclStudyM# 5010-W Exposure Chambers Incubation Observations Endpoint Calculations 7@pe: 4-L glassjars. . Use in test:3.0 L of dilutino wateror testsolution/chamberD.uring the test,kept covered,exceptwhen experimentalobservationwsere made. Solutionswere not aeratedduringthe exposures. Duration: 96 hours. Daily photoperiod: 16 h lightand 8 h dark,maintainedusingcool white fluorescenltamps. Temperature: 21.0 1*C. Biological:Daily- Test organisms with abnormal behaviorand signsof stress,and dead testorganisms. Water chemistry: (1) At testinitiatiotno-talhardnessand alkalinity(,2) daily-temperature,DO, conductivitya,nd pH. Alldeterminationwsere made accordingto APHA methods (1995). LCIO: InhibitioCnoncentratio(nlCp)Method. LC50's: Trimmed Spearman-Karber Method. NOEC- Fisher'sExact Test (TOXSTAT statisticsaolftware,Version3.5, -estInc.,and Universityof Wyoming). Sponsor:3M Company SponsorStudyTD# R1904 4 ASelCosporation/ASel-Dukdh Env4vnummW TeafingDi%iaion AUI StudyM# 5010-M 3.0RESULTS TestSubstm= Toxicit(yPerd=t dataarein Table 1) 24-hLC50: > 250 mg/L 7 (NotCalculable). 48-hLCSO: 211 mg/L (NotCalculable). 72-hLC50: 146 mg/L (128-167). 96-hLCIO: 45 mg/L (22-80). 96-hLCW: 97 mg/L (91-116). Ranges of Waw ChemistryParameters 96-hNOEC. 54 mg/L. Totalhardnas (asCaCO3 mgll):46-48. A&dinity (asCaCO,3 mgll): 44-46. Teinperatur(e*C): 20.8 (No Range). DO (mglL):5.4-8.8. pH.- 7.60-8.05. isp!!dlcLo@@ (yn-ftsLa-nJ1:16-133. Spomor. 3M CompiLay Sponwr Study 0# R1904 5 ASclCOrpomtion/ASci-Duluth F-nvirownenTteasltinDgtv*nion ASciStudyM# 5010-M 4.0TEST QA/QC QA/QC Criteria Mortalityincontrolexposuremust notbe more than 10%. Data 0% mortality. The lowestDO concentratioant test terminatiomnust be atleast60% of theair saturatiovnalueat testtemperature. The lowestDO concentratiomneasured was 61% of theairsaturatiovnalueat2 1.0*C. Testdurationmust be 96 h. The t---d.utrationwas 96 h. ReferenceSubstanceToxicityTest (thistestis Controlchartforsodium chloridereference optional). I toxicittyestinigsin Raw Data Package. 5.0REFERENCES American PublicHealthAssociatio(nAPHA). 1995.StandardMethods fortheExaminationof Water and Wastewater.APHA, Washington,D.C. OrganizatiofnorEconomic Cooperationand Development(OECD). 1981/1993.OECD GuidelinesforTestingof Chemicals.OECD PublicatioInnformatioCnenter,Washington,D.C. Hamilton,M.A., R.C. Russo,ahd R.V. Thurston.1977.Trimmed Spearman-KarberMethod for EstimatinMgedian LethalConcentrationisnToxicityBioassays.Environ.Sci.Technol.11(7): 714-719;Correctio1n2 (4):417 (1978). USEPA. 1993. A LinearInterpolatiMoenthod forSublethaTloxicityT:he Inhibition Concentratio(nlCp)Approach. Version2.0. West Inc.,and Universityof Wyoming. ToxstatSoftwarePackage.Version3.5.Cheyenne, Wyoming. Sponsor: 3M Company Sponsor Study ID# R1904 6 TABLE 1. AC= ASel Corpomtion/AScl-DuUh EuvirOAnSuemInStWudTyclotDin#gSODlivO-@Ma TOXICrrY OF R1904 TO FATHEAD MINNOWS (P.promelas) 0 (control) 19 ......... 0(0) 0(0) 32 0(0) 54 0(0) 90 0(0) 0(0) 250 0(0) Pacmtages areinpareadwd& .. ..... 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 15(75) 0(0) 0(0) 0(0) 0(0) 2(10) 9(45) 20(100) 0(0) i(S) 0(0) 3(15) 8(40) 16(80) 20(100) LETELA-LAND NO-EFFECT CONCENTRATIONS WrM CORRESPONDING 95% CONFIDENCE INTERVALS ................ .......... 24-h LCSO > 250 ....... Not calculable 49-h LC50 211 Not reliable 72-h LCSO 146 128-167 96-H LCIO 45 22-80 96-h LCSO 97 81-116 96-h NOEC 54 Not applicaalbeblel a Values booed on nominal testcow-enhifions. Sponsor: 3M Company Sponsor Study M# R1904 7 ACUTE TOXICITY TO AQUATIC INVERTABRATES TEST SUBSTANCE (DAPHNIA MAGNA) Identity:Perfluorooctanesulfonylamido(ethyl)acetmaatye;also be referredto as PFOSAA, FC-128 or as the major component of FC-1 27, FC-1 29, FC-1 09, FC-1 09-X or Rl 904. (Glycine,Nethyl-N-[(heptadecafluorooctyl)sulfopnoytla]s-s,ium salt,CAS 2991-51-7) Remarks: The testsample isFC-129 referretdo by thetestlaboratory as R1904. Currentinformationindicatesitisa mixtureof42% test substance,32% water,14% 2-butoxyethanol4,% ethanol,and 8% C4-C7 fluoroalkyclarboxylatecompounds. Test materialwas from 3M production lot930014, mfg. Date 3/97. The followingsummary is abbreviated because the testsample isa mixture of the testsubstance ina waterlorganicsolvent solution. Data may not accurately relatetoxicityof the testsample with thatof the testsubstance. METHOD: Methods: OECD 202 Test type: Staticacute GLP: No Year Completed: 1997 Species: Daphnia magna Analyticalmonitoring:Temperature,pH, DO, and conductivity RESULTS Nominal concentrations: Bk control,1.4,2.3,3.9,6.5,10.8,18,30, 50 mg/L (induplicate) Element values: 24-hourEC50 = >50 mg/L (C.I.notcalculable) 48-hourEC,o = 4.4 (4.3- 4.6)mg/L 48-hour EC50 = 9.1 (7.4- 11) mg/L 48-hour NOEC = 3.9 mg/L Allelement values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureofthe testsubstance as describedinthe testsubstance remarks field.The value reportedapplies to thatmixtureand notthe testsubstance. No attemptwas made to determinethe impact ofthe presence ofthe organicsolventsorwhat portionofthe toxicitcyan be contributedtothe Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC-1 29 48-hour EC5o forDaphnia magna was determined to be 9.1 mg/L witha 95% confidenceintervaolf7.4 - 11 mg/L. The 48-hour no observed effectconcentrationwas 3.9mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking2. Testingmeets allcriterifaorquality testingb,ut lacksanalyticaclonfirmationoftestsubstance concentrations. There isa lackofcharacterizatioonfthe testsample. REFERENCES This study was conducted atASci Corporationof Duluth,Minnesota atthe requestofthe 3M Company. OTHER Last changed: 5/17/00 ASelColponfiWAScl-DuMb EnvbomnentdTcodngDWWou ASelStudyMO 5010-068 STUDY ACUTE TOMCrrY OF R1904TO DAPHNL4 MAGNA DATASTANDARD OECD GUIDELINE 202 STUDY COMPLETED June 17, 1997 TESTING FACILITY ASCI Corporation/AScl-Duluth EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,MN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTRFICATION NUMBERS AScl Study ID# 5010-068 3M Company Lab Request#R1904 Sponsor 3M Company Sponsor Study B)# R1904 ASelCorpomtion/AScl-Duluth EnvironmentTaelstinDgivision AScl StudyID# 5010-068 1.0GENERAL EQFORMATION StudyTitle DataStandard Sponsor gponsor'sRepresentative Sponsor'sLab Request# TestingFacility PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitivTeest Dates Data Validationand ReportReview AcuteToxicitoyfR1904 toDaphniamagna. OECD Guideline202. 3M EnvironmentalTechnology and Safety Services,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133. Susan A. Beach, 3M Environmental Technology and SafetyServices,Building 2-3E-09,935 Bush Avenue, St.Paul,MN 55133.Tel No. (612)778-7452. R1904. ASCI Corporation/AScI-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811;Tel.No. (218)722-4040. HoflyKitterman. Joe Amato. Donald Mount. June 10-12, 1997. Alan Mozol. R epo rtSi nature- Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport Two yearsfrom the datestudyiscompleted. ASCI Corporation/AScI-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811;Tel.No. (218)722-4040. Sponsor: 3M Company Sponsor Study ED# R1904 2 ASciCorponifion/ASel-Duluth EnvironmentTaelstinDgiviska ASelStudyIDI 5010-068 2.1 OB.IIICTIVEISI To deteerrmmineethe48-h ECIO and EC50, and 48-h NOEC (no observedeffectconcentrationo)f thhee] teusjtssuubbbssttaannccee,R1904, forDaphnia magna. Th0its stuedy2ww0aa2ssthcc'onductedaccordingto theAScl studyplanretainedby theSponsor and OECD GEGduidneel7ine202 (OECD 1993). 3.0 TEST MEETHODS Test Substance Test Organisms DilutionWater Exposure Chambers Properties:R1904, yellow-coloredliquid,specificgravityCa. 1.3 (water 1),stable,and completelysolubleinwater. Testconcentrations0: (dilutiownatercontrol)1,.4,2.3,3.9,6.5,10.8, 18, 30, and 50 mg/L. Stocksolutiopnreparedby dilutin2g00 mg of test substancein 1 L of dilutiownater. Test substanceconcentrationwsere preparedby directadditionof stocksolutioinntodilutiownater. A 0.6 dilutionserieswas utilized. Source: Healthy,lessthan 24-h old Daphnia magna neonatesobtained from ASCI stockculturesmaintainedinwaterof testdilutionwaterquality. Use intest:Gravid adultsisolateidndilutiownater24 h beforethetest initiation-.Used5 organisms/replicaatned 4 replicates/tceosntcentration. Handled with a smooth bore pipetwithan insidediameterof 6 mm. Did not feedduringthe test. Source: DechlorinateCdityof Duluth,MN, tapwater. The most recent chemicalanalysisisinRaw Data Package. CharacteristicsA:t testtime,the waterhad a hardnessof 46 mg/L (as CaCO3) and a pH of 7.93. Aeratedfor24 h priortousingin thetest. Type: 250-ml borosilicatgelassbeakers. Use in test:200 ml of dilutionwater or testsolution/chamber.During the test,kept covered,exceptwhen experimentalobservationwsere made. Sponsor3-M Company SponsoSrtudyID#R1904 3 ASEICorpomtion/ASel-Dulugh EnvironmentalTestingDWkion ASel StudyID# 5010469 Incubation Observations Endpoint Calculations Duration4:8 h. Dailyphotope?iod1:6h lighatnd8 h dark,maintainuesdingcool-white fluorescenbtulbs. Te?nperature:21*C. Biological-D.aily- gentlyagitatedtheexposure chambers and then counted immobilizedand dead testorganisms(immobilizedorganismswere those thatdid not swim within15 secondsafterexposurechambers were gently agiud,ed).Did not remove any daphnidsfrom theexposurechambers at 24 h. Water chendstry:(1)At testinitiatiotno-talhardnessand alkalinity(,2) daily-temperaturep,H, DO, and conductivityA.U determinationwsere made accordingto APHA methods (1995). ECSO: Trimmed Spearman-Karber method. ECjo: InhibitioCnoncentratio(n10%) USEPA softwarepackagefor DeterminingICp concentrations. NOEC,. Fisher'sTest (TOXSTAT statisticsaolftware,Version 3.5,West, Inc.,and Universityof Wyoming). 4.0 RESULTS Test SubstanceToxicity(Pertinendtataare in Table 1) 24-h EC50: > 50 mg/L. 48-h EC50: 9.1 mg/L (7.4-11.0). 48-h ECIO: 4.4 mg/L (4.3-4.6). 48-h NOEC. 3.9 mg/L. S ponsor. 3M Company Sponsor Study M# R1904 4 ASel Corponition/ASel-DubA Envimaw4ntal TestingDivision ASel Study M# 5010-068 Ranges of Water ChemistryParameters Totalhardness (asCaCO3 mgll): 44-46. A&dinity (as CaCO3 mgll): 42-44. Temperature(*C): 20.5-20.8*C. DO (mglL): 8.1-8.5. PH.- 7.81-8.04. kLc7LC E225L,@ 6okslc)nj: 118'-126. 5.0 TEST QA/QC QA/QC Criteria Testorganismdead, immobilizedor trappedin controlexposuremust not be more than 10%. Ile lowestDO concentratioanttest terminationmust be atleast60% of theair saturatiovnalue at testtemperature. Testdurationmust be 48 h. Referencesubstancetoxicittyest(thistestis optional). Data 0% dead,immobilizedor trapped. The lowestDO concentrationmeasured was 91 % of the airsaturatiovnalue at 21.OOC. The testdurationwas 48 h. CurrentcontrolchartforD. magna acute testingisincludedin theraw datapackage. Sponsor- 3M Company Sponsor Study IDO R1904 5 ASR-ICorporation/AScl-Duluth EnviroamenWTestinDgivision ASciStudyMO 5010-068 5.0REFERENCES Amencan PublicHealthAssocmtion(APHA). 1995.StandardMethods fortheRumination of Water and Wastewater.APHA, Washington,D.C. Hamilton,M.A., R.C. Russo,and R.V. Thurston.1977.Trimmed Spearman-KarberMethod for EstimatingMedian LethalConcentrationisn ToxicityBioassays.Environ.Sci.Technol. 11(7):714-719C;orrectio1n2(4):417(1978). organizationforEconomic Cooperationand Development (OECD). 1993.O.ECD Guidelinesfor Testingof Chemicals.OECD PublicatioInnformationCenter,Washington,D.C. West Inc.,& Universityof Wyoming. TOXSTAT SoftwarePackage Version3.5. US EPA. 1993.A LinearInwzWlationMethod forSublethaTloxicity:The Inhibition Concentratio(nlCp)Approach (Version2.0).EnvironmentalResearchLaboratory,Duluth, Minnesota. Sponsor- 3M CompiLny Sponsor Study ID# R1904 6 AScl Corpomtion/ASel-Duluth EnvironmenW TestingDivision ASel Study M# 5010468 TABLIE 1. ACUTF, TOXICITY OF R1904 TO DAPHNLI MAGNA ................ ..... .......-..-...-......-.....-.....-.....-......-...-..-...-.....-..-...-..................................................................... ........ .......... ... ........... ................ . .....z4- ........:......................... 0 1.4 2.3 3.9 6.5 10.9 is 30 50 0(0) 0(0) 0(0) 0(0) 0(0) 1(5) i(S) 0(0) 4(20) .......... ..... .......... 0(0) 0(0) 0(0) 0(0) 10(50) 17(&5) 14(70) 16(80) 20(100) Percentagesare inparenthesis. EFFECT AND NO-EFFECT CONCENTRATIONS WrM CORRESPONDING 95% CONFIDENCE MTRVAIS d NOtdi@'; 24-h ECSO 48-h ECSO 48-h ECIO 48-h NOEC m .............. ...... D ....................................... > so Not Calculable 9.1 7.4-11.0 4.4 4.3-4.6 -3.9 Not AppiLcable Sponsor. 3M Company Sponsor Study M# R1904 7