Document jL7Dd93M89VE2NQXwab81xK5
ACUTE TOXICITY TO FISH
AR226-053Y
TEST SUBSTANCE
Identity: Paelrsfoluboeroroecfteyrlrseudlftoonaaste,Flduoirdoeaclykylldiammemtohynliaummmodneiriuvmatsiavlte;. m[a1-y Decaminium, N-decyl-N,N-dimethyl-, salt with 11,2.2,33,4,4.5,5,6,6,7,7,8,8,8-heptadecafluoro-1octanesulfonic acid (1:1), CAS # 251099-16-)
Remarks: The 14394 referred
3M production lot number was Lot to by the test laboratory as P3025.
1. The test sample The sample was,
is
L-
ilnabwealteedrF(-a1p1p6r1o5x,imLaottel1y. 3T0h-e40te%sttessatmspulbestisanacem,ix6t0ur-e7o0f%twhaeteters,tasnudbs0t-a5n%ce
of residual perfluorochemicals). The test sample appears to be a
All values reported relate to this 2-phase dispersion (clear liquid
mixture. with
opaque solid) were made to
which adjust
rapidly for the
separates after agitation. No calculations actual concentration of the test substance
in
the test sample.
METHOD
Method: OECD 203
Type: Static acute
GLP: No
Year completed: 1996
Species: Pimephales promelas
Supplier: Not noted.
Analytical monitoring: monitored daily.
DO,
pH,
temperature, and
conductivity were
Exposure period: 96-hours
SStpaetairstmiacna-lKmaertbheordmse:thLoLds.o vTahleueNsOcEalLcuwlaatsedcaulsciunlgattehdeuTsriinmgmFeidsher's
Exact tests.
Testfish age: Not given.
Length and weight: Average length = 11.3 mm, Average weight = 7.8
mLogading: 0.26 glL
Pretreatment: None
Test conditions:
Dilution water:
DweacshlaoerriantaetdedfoCri2t4y-ohfoDuurlsutphr,iorMtNo
tap use
water. in the
Water test.
C042C2
Dilution water chemistry:
hardness: 48 mg/L as CaCO,
pH:
8.08
Lighting: lCigohotl,-8w-hhiotuerfslduaorrekscuesnetd.bulNbos.trPanhsoittoiponerpieordioodfn1o6t-ehdo.urs
fSrtaoctcikonasn. dTetsetstsosloultuitoinosnwperreepaprraetpiaorne:d
Water accommodated individually for each test
sreupblsitcaatnececoinnc4enLtroaftidoilnutbiyonmwaastser.addTihtieonsoolfutviiognosrowuesrley svihgaokreonustleyst
spthiarrseed wfoars21s-ihpohuornsed(vforrtoemxt1h/e2vteoss1e/l3 astolmuitdi-odnepdtehp.th). The aqueous
CStoanbcielinttyroaftitohnestedsotscinhgemriactea:lOsnocluetions: Not noted.
EThxepojsarusrweevreessseelasl:ed 4w-iLthglTaesfslojna-rlsinceodntlaiidsnifnitgte3d-Lwiotfh tsetsotpspoelurtsiotno.
accommodate oxygen flushing of headspace.
Number of replicates: two
NNuummbbeerroofffciosnhcepnetrrarteipolnicsa:tet:htreene plus a negative control
Water chemistry during the study:
Dissolved oxygen range (0 -- 96 hours):
9.1 14.6 87-182
mglL mg/L
(control exposure) (700 mg/L exposure)
pH range (0 -- 96 hours)
7.80 -8.08 (control exposure)
Test
7.78799 temperature
(700 mg range (0 --
exposure) 95 hours)
20.8-20.9C
Conductivity range (0 - 96 hours):
128 -- 142 umhos/cm (control exposure)
118 - 154 mhos/cm (700 mg/L exposure)
bReefmoarreksse:aliOnxg yignietinalwlyaasnaddadteedactoh tohbesehrevaadtsiponacpeeriinodt.heTjhares tdeisstsovlevsesedlos,xypgaertniccuolnacrleyntinratthieon7s00wemrge/sLuepxeprossautruerated in the concentration.
RESULTS
Nominal Element
loading concentrations: value: 24-hour LLso = 618
Bk control, 400, 700, (568 - 673) mg/L.
1,000
mg/L.
48-hour LLso = 607 (554 - 664) mg/L
C042C3
72-hour LLsp = 595 (551 - 643) mg/L 9886--hhoouurr LNLOsEo L= 5=6<24(9502m3g-/6L04) mg/L
All element values based on nominal concentrations.
Biological observations after 96-hours: No mortality or abnormal behavior observed in the negative control during the test. Mortality was
oohobbussreesrr,vveeaddndiinn2thfahilesfhorfewmetarhieenifqniusgiheeasxtcpetonhsteua7rt0e90c6o-mnhgco/euLnrtser.xaptNiooosnusar.benSocuorrnmfcaaelcnitbnregahtawivaoinsoartw2a4sobserved in the 400 mg/L exposure concentration.
Cumulative percent mortality:
LCoosinnng oTons| 20n0us | spurs | 7200s
hoomal |&| 5|,| [oo[0[6[6| | FmTw[oe[wo [w] Coo[0| wo| 00 | ww |
Lowest concentration causing 100% mortality: 1,000 mg/L Mortality of controls: None
Remarks: Values reported are for the test sample. No calculations were
made to adjust for the concentration of the test substance in the test sample.
cCoONNoCwLuUsSiIOoNnSs The test sample 96-hour LLsofor fathead minnow was determined to be 5no62obmsge/rLvweidthefafe9ct5s%lecvoenlf(iNdOenEcLe)iwntaesrv<al49of0 5m2g3/L-.604 mg/L. The 96-hour `Submitter: 31 Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, Minnesota, 55133
ba0 DAtTaAQQuU0 AALbIyTYy 000000
Reliability: Kiimisch ranking 2. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is
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C04R35
EAn5i4r1oCrompeonSrsatiisToonn0ii0AnSgSeBDoruetnan
STUDYTITLE
ACUTE TOXICITY OF `33% DEVELOPMENTAL MATERIAL TO FATHEAD MINNOW (PIMEPHALES PROMELAS)
DATA STANDARD OECD GUIDELINE 203
STUDY COMPLETED
January 2, 1997
TESTINGFACILITY
AScl Corporation/AScl-Duluth Environmental Testing Division
4444 Airpark Boulevard
Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592
STUDYIDENTIFICATIONNUMBERS
AScI Study ID# 5010-068
3M Company Lab Request# P3025
SSpoonmsaorn, Sl3yM'C1o0mpPanyS
1
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EancvliAoCnSomrepSnoaruadiToon1s/8AiS0cD1-0Di.uo5t0un8h
1.0 GENERAL INFORMATION
Study Title
i
1|
| `Sponsor's Representative
Sponsor's Lab Request?
li Testing Facility
I
Principal Investigator
Acute Toxicity of P3w< Developmental
pMraotmeerliaasl).to Fathead Minnow (Pimephales
OECD Guideline 203
S3eMrviEcnevsi,roBunimlednitnagl 2T-e3cEh-n0o9l,o9g3y5anBdusShafAevteynue,
St. Paul, MN 55133.
|
Susan A. Beach, 3M Environmental `Technology and Safety Services, Building 2-
I
[ps 3E-09, 935 Bush Avenue, St. Paul, MN 55133, Tel No. (612) 778-7452.
TAeSsctlinCgorDipvoirsaitoino,n/4A4S4c4I-ADiurlpuatrhk EBnovuilreovnamred,ntal
[oeDierkes| Duluth, MN 55811; Tel. No. (218) 722-4040.
Testing Facility Director
Definitive Test Dates Data Validation and Report Review
Donald Mount.
December 17-21, 1996.
Alan Mozol,
Be
Retention of Raw Data and Final Report I Location of Raw Data and Final Report
1
_
Two years from the date study is compl4eted. |i `ASel Corporation/ASel-Duluth Environmental
Testing Division, 4444 Airpark Boulevard,
Duluth, MN 55811; Tel. No. (218) 722-4040.
SSppoonnsioorr: Su3byCIoDmspPaangyes
2
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+
EAuSneilroCnmoemnpiosrlaiToensttaiSneglD-Diuvliustohn
`ASel Su 104 5010-068
2.0 OBJECTIVES)
Tteostdseutbesrtmainncee,th#e 7965-h LDLe,vel(loeptmhaelntlaoladiMantge)raianld, 9f6o-rh fNatOheEaLd m(nionnoobswer(vPeidmeepfhfaelcteslopardoimnegl)aso).f the
`GTuhiidsesltiundey2w0a3s(cOoEndCuDcte1d993a)c.corTdhiengtetsot
the AScl
solutions
study
were
wplaatnerr-eatacicnoemdmboydattheedSfproancstoiornsan(dWOAFE)CD
||
prepared following Girling, Whale, & Adema 1994.
|
3.0 TEST METHODS
[| rest substance I || | Test Organisms I
Pordooprelretsisess:lurr3yca>ft5er
a,gittwaot-iponh,assepelciiqfuiicd
before
gravity
acgai.ta1t.e2d,g/lcicg,htacnodloirnesdo,lunbelaerly
|
in water.
Tmegs/tL.loaTdeinsgts:loa0di(ndgilsuotliuotniownastewrerceontprroelp)a,re4d90inmdgiv/iLd,ua7ll0y0 fmogr /eLacahndtes1t 000
replicate. vigorously
The 490 mg/L solutions shaken test substance in
were prepared 4 L of dilution
by placing 1960 mg of water. The 700 mg/L
and and
1000 4000
mg/L solutions were mg of test substance,
prepared in the same manner using 2800 mg respectively. The solutions were vigorously
stirred (vortex
siphoned from
1/3
the
to 1/2
vessel
astolmuitidondedpetphth)
for 21
h.
The aqueous phase was
|
| Source: Healthy, juvenile fathead minnows (Pimephales promelas) obtained from a commercial supplier.
Holding/acclimation: Held and acclimated for 12 days in the test dilution
water. entire
For the 12 days,
48 hours mortality
preceding was 0%.
the At
test test
0% mortality occurred. For termination, the fish were 1.1
the
cm in mean length and 7.8 mg in mean weight
Use in test: Used 10 organisms/replicate and 2 replicates/test concentration.
SSppoonnssoorr: Su3dMy CIoHmpPa3n0y25
3
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[I pitution Water
Exposure | chambers |l | |
Observations
|
Endpoint Calculations
EAnSveilcAoCnSmSoenriSaptl yoTre1n0a3gt0iD1Di0ovu.itns0ia6so8n
Scohuermciec:al Daencalhylsoirsiniasteind RCaitwy DoaftDaulPuatchk,agMe.N, tap water. The most recent
CChaarCaOct)eraisntdicas:pHAoft8e.st08t.imeA,erthaetewdatfeorr 2ha4dhaphraiorrdnteossusoifng48inmtgh/eLfes(ta.s aTcypceo:mm4o-dLatgelaossxyjgaresnsfelaulsehdinwgiotfhhTeeafdlsopna-lcien.ed lids fied with stoppers to
tUesste, ikneptetstc:ove3reLd,ofedxicleupttiownhweanteerxpoerritmesetntsaolluotbisoenr/vcahtaimobnesr.werDeurmiangdethoer |
headspace exposures.
was
flushed
with
oxygen.
Solutions were not aerated during the
Duration: 96 h.
Daily photoperiod: fluorescent bulbs.
16 h light and 8 h dark, maintained using cool-white
Temperature: 21C.
Bsitroelsosg,icaanld:
Dailydead test
Toersgtanoirsgmas.nisms
with
abnormal
behavior
and
signs
of
dWaaitley-r tcehmepmeirsattruy:re,(1D)OA,tctoensdtuicntiitviiattyi,onantodtaplH.hardAnlelsdseatenrdmianlkaatliionnisty,we(r2e) `made according to APHA methods (1995) 2o4b-s,er4v8a-b,le72e-f,feactndlo9a6d-ihngme(dNiOaEnL)lethal loading (Lys), and 96h no
LLgy Trimmed Spearman-Karber method. WNyOoEmLi:ngT.OXSTAT statistical software, Version 3.2, University of
SSppoonnssoorr: Su3dMyCDoAmpPa2n0y25
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EAnvtrAoCmSomapSroaaryTions1Ai0n8SgeSoD1D0ou00nt8
3.0 RESULTS
Test Substance Toxicity (Pertinent data are in | 24-h LLyy: 618 mg/L (368-673 mary, ]
| Table 1)
48h LLgy: 607 mg/L (554-664 mg/L).
|
|
72h LLgy: 595 mg/L (551-643 mg/L).
|
96h LL 562 mg/L (523-604 mg/L).
{
Ranges of Water Chemistry Parameters
|
96-h NOEL: <490 mg/L.
Total hardness (as CaCO, mg/L): 44-43.
Alkaliniy (as CaCO, mg/L): 44.
I
Temperature (C): 20.8-20.9C.
{
|
DO (mg/L): 8.6200.
PH: 7.71-8.08.
-
Specific conductivity (umhos/em): 118-487. |
4.0 TEST QA/QC
| Morality in control exposure must not be more| 0% dead. than 10%.
The lowest DO concentration at test
staetrumriantaitoinonvamluusetatbeteastt tleeamspter6a0tu%reo.f the air
{
| SSphoonmsoerr. S3tMyC0o4mpaFancyes
The lowest DO concentration measured was
9Af7t%eroifnittihael a0i-rhsoautrurraotuitoinnevaclhueemiasttr2i1e.s0hCa.d
ebaecehn cphearmfboerrm'esd hteheadcshpaamcbeewrassweflrueshseedalweidthand
oxygen daily Ensuing DO
to maintain adequate DO, concentrations were 100%
of
saturation or greater.
s
G04R40C
Test duration must be 96 h.
oRpetfioenraeln)c.e substance toxicity test (this test is
EAriSrNoCmSommepSnoaryiToon1/s0A4iSSeD1Dium0to8an
"The test duration was 96 h.
Control
toxicity
chart for
testing is
sodium
in Raw
cDhaltoariPdaeckraegfee.rence
5.0 REFERENCES
| American Public Health Association (APHA). 1995. Standard Methods for the Examination of
| Water and Wastewater. APHA, Washington, D.C.
| Girting, AE. G.F. Whale, and D.M.M. Adema. 1994. A Guideline Supplement for Determining the Aquatic Toxicity of Poorly Water-Soluble Complex Mixtures using WaterAccommodated Fractions. Chemosphere, Vol. 29, No. 12, pp. 2645-2649.
Hamilton, M.A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for
Estimating Median Lethal Concentrations in `Toxicity Bioassays. Environ. Sci. Technol.
| 11(7):714-719; Correction 12(4):417 (1978).
1
Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C.
{Universityof Wyoming. 1996. Toxstat Version 3.2. Laramie, Wyoming.
SSppoonsoorr: uItMy CIofmpaP3n0y25
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E5n1rCoopanoyrTnosiotnabioauaost
TABLE 1.
ACUTE TOXICITY OF 73+" MINNOWS (P. promelas)
DEVELOPMENTAL MATERIAL TO FATHEAD
or [TestLosvuibsnugnee
= =[a Cumulative Number of Dead Organisms and Percent Moralin |
a=t Tse e t |ose esTToww e T|a wo) o]
[EES--
ee NOT beeen] eam EFFECT AND NO-EFFECT LOADINGS WITH CORRESPONDING 95% CONFIDENCEINTERVALS
me
Tw]
Coen To 2
SSyomonna, St38y8 C5o8mpPaneys
,
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