Document jBaROyabrExRN9Mz45nJQx6p
AR26- 0496
BIOCONCENTRATION
ITEESTSSUUBSTANNCCEE
000000000
Identity: PPeFrOflAuoarmoomcotnaniouimc ascailtd,,Aammmmoonniiuumm psaelrtf;lmuacryoaolcstaoncbaeter,efPeFrrOe,d FtoC-as
116, FC-126, + ammonium
FC-169, or salt, CAS#
3F8C2-51-4236.-1()Octanoic
acid,
pentadecafiuoro-
Remarks: The test sample is FC-143. It's purity was not sufficiently
c1h0a0ra%ctteersitzseud,bstthaonucgehacnudrr0en--t3i.n5fo%rmCast,ioCn7,inadnidcaCtesspietrifslauomroixatnuarleoogfu9e6.5 -
compounds. The 3M production lot number 377.
weMEVTWHOOOD::
tMheethToedchingiuciadlelRienpeorftolSluomwmeda:ry3.M Idnevreisvteidgaptrieolnimiinncalruydeidnvmeesttihgoadtiodnevoeutlloipnmeednitn fToyrpteh:e Satnaalliycseisxpoofsfulruoerwidiethbystactoimcbculsetairoann.ce phase. GLP (YIN): No Year: 1995
SSuppepcliieesr:: AFqautahteiacdBmiionsynsotwem(sP,imIenpc.haFloerstprCoolmlienlsa,sC)O.
Analytical monitoring: Whole fish and test solutions were combusted in a
modified organic halide analyzer (Dohrmann DX-2000). F"activity in collection
fOlruiidonwaRsestehaernchmeiaosnuarnealdyzweirt.h aTnhieonc-osnecleencttriavteieolnecoftrPodFeOcwonansecthteend ctaolacunlated
fLreonmgtthheaFn"diowneicgonhcte:ntNroattinoont.ed in the report.
DUepptuarkaetpieorniopde:rio1d3: d1a5ydsays
S(taaltpihsati=ca0l.0m5e)twheordes:useOdnet-owcaoympaanarleystihse oafvvearraigaencceonacnedntTruakteiyon'ss oHfSPDFtOesitn
tissues from PFO-exposed Test fish age: 64 days
fish
during
the
uptake
and
depuration
experiment.
Loading: 124.6 + 20 mg fish (wet wt.) per liter test solution.
TPersettrceoantdmietnito:ns:Not noted.
Dilution water: Carbon-filtered well water from 3M Well #2, St. Paul,
DMiNlution water chemistry:
halakradlinneistsy:: 227322+%88 mmggi/LL aass CCaaCCOOs3
pCHo:nductivity: 58150%0+.114 pmhos/cm
Dissolved oxygen: 8.5 +0.3 mg/L
03708
SprteopcakreadndbytedsitsssoollvuitnigosnolpirdepPaFrOatiinondi:luTthioen pwraitmearrytosctroecaktseolaut1i0o0n0was
mobgt/aLinceodncbeyntdrialtuitoinng. aTnhaelinqoumotinoaflthceonscteonctkrastoiloutnoiofn 2to5 1m5g/liLtewrsaussing
dilution water.
Exposure containing
vessels: 5 15 liters of
tgeasltlosonlcuytliionnd.rical
high
density
polyethylene
tanks
Number Number
of of
replicates: One fish per replicate:
30.
WNautmebrecrhoefmicsotnrcyendturraitnigontsh:e
One plus study:
blank
control
dDriospspoeldvebdeolxowyg6e.n0:mgT/hLeaDtO72cohnocuernstraafttieornthoef stthaertcoonfttrohle test.
AteesrtattoiomnaiwnatsaiinniDtiOateldevaetltshaits poorinnetaarnd10c0o%ntsiantuuerdattihorno.ughout the
TAevsetratgemepperHat(cuornet:ro2l0a+1ndCtest solutions): 8.2 +0.2
Photoperiod: Feeding: Fish
16 hours were fed
light and live brine
8 hours shrimp
dark. every
48-hours
beginning
24-
hours after Sampling:
starting the test. Five fish were removed
from
each
tank
at
192
and
312
ahfotuerrsbediurnigntgratnhsefueprrteadketopchlaesaen adinldutaiognaiwnat2e4r,. 96, 168, and 360 hours
nReeemdaerdk.sDfiOelmd:eaFsecuarlemmeatnetrsiawle/reextcaeksesnfdoaoildy.waTsesrtemsoolvuetidonfrpoHm
tanks was
as
measured once more at the end of each experimental phase.
03709
= 0 RES, U0 LTS 00000000000
Concentration of PFO measured in test solutions.
SoPlhuatsieon/ UPTAKE
C`oAnvceernatgreaPtFioOn | Standard
(ug/ml)
Doviation
Control
[1392
<<
-:
312
<t
:
25 ugimL PFO
1802
225505
2128
"DEPURATION
312
259
18
Controlsoclluetairoannce tank 1608 3680
<<1t Not avaiable
25 ugcliemaLraPnFcOe teaxnpkosed 1608 3680
Not a<<vatiable
Elapsed time (hours) from the beginingof each experimental phase.
Average tissues
concentration
of
PFO
(measured
as
F-)
in
Pimephales
promelas
(THioumros)
volg
PROT Fish Standard
omparison of
wet weight Doviation
Means =
Tir
es
Tro
8 [Sse
sr
er
17a9 7Ts
ra TAT
see
75
5115
15
Tc
F2** N0 Muemabnesr0 wnith()tihnedsicaamtees|lefltarpsaerde8tnmot0esi(gHnoiufrisc| )anrtolymdbiefgeirne1nnti6natgoaflpdheap| =ur0a,t0i--5onby| pthhae-- se | `Tukey-Kramer Honest Significant Difference test
TwhheercealCc,uliasttehde3e1q2uihlioburriuBmCcFonfcoernPtrFaOtiiosn1o.f8tuhseitnegstthseuebqsutaatnicoeniBn CthFe =fiCsh,/anCd,
Cu the
is the equilibrium concentration organism is exposed.
of
the
test
substance
in
the
water
to
which
03710
eCe ONCLn USIs ONS
No definitive conclusions can observations can be made.
be derived from this study.
General
PTPhFFeOOPcinFontOcheeinnttferisasthtsitooilnsusstuiioennac.rpepaesaerdedin ttohebefidsihretcitslsyuerseloavteedr ttohethceoucronsceeonftreaxtpioosnuroef.
tWhheilcelecaornacnecnetrpahtaisoen,s tdheecrteimaeserdeqiunitrheed wfohrocloemfpilshetseamrpemloevsaolviesrltohnegeprertihoadnof the 15 days used in the test.
The concentration of PFO exposure experiment.
in
test
solutions
remained
constant
during
the
static
Relative exposed
to to
the control PFO were
treatment, the survival not adversely affected
daunrdinggrotwhtehexopfetrhiemetenstt.fish
Submitter: 3M Paul, Minnesota,
Company, 55133
Environmental
Laboratory,
P.O.
Box
33331,
St.
elDAATQAuQUAAtLIlTYY
tReesltiianbgi,lihtoyw:evKeirm,istchehrerawnakisngno2. deTfhiniistisvteuadnyalmyeseitssotfhtehecrcitoermipaofuorndquailnietiyther ntohte afilslhoworfotrhethteesdtestoelrumtiinoantsi.onItowfadsegmreaadsatuiroendoirndmierteactbloylaissmF-biyopn.rodTuhcitsswaonuldd pcoosuslidbiyliietlydtfhaaltstelhye htiesgthsruebasditnagnsc.e Tmhaeyrehawvaesbaelesno nsoorcboendsitdoetrhaetisounrffoarcethoef the sfitsuhdryaltahcekrsthcahnarraecstiedriinzgatiinotnemoaflltyh,e ttehsutsspuobsssitbalnyceovpeurreitsyt.imating the BCF. The T(hoibrsderpvaarttiyonrseviinecwlubdyedDrw.itJhohrnepoGrite)svyeroiffiGeisetshye Esctoutdoixeiscosltorgeyn,gtIhnsc.and weaknesses.
S REFERENCES ees 000 A3mMmToenchinuimcaPlerRfelpuoorrtoo"cAtsasneatses:meStnattoifc"tRh.eD.BiHooawceclulm,uJl.aDt.ivJeohPnrsoopenr,tiJ.eBs.oDfrake, RD. Youngblom, May 31, 1985
P3eMrfrleuqouroeosctteadneoxatpee:rt SotvaetrivciFeiws,h "TBeisot"a,ccDurm.uJlaothinveP.PGroepiesrytioefsGoefiAsymmonium Ecotoxicology, Inc., March 20, 1995
03711
-OT_HER Last changed: 5/24/00
O00
03712
Technical Report Summary
Toop:rPSataunt &mmTuesmcthbneaicpaerlwtCeyorm,muG nicatu iaornest nSeerrvies ceis ,-201.212
TawDe.L. BACON
i R. D. HOWELL, J. D. JOHNSON, J. B. DRAKE, R. D. YOUNGBLOM
or
Fon ENVIRONMENTAL > TECHNOLOG&Y SERVICES
089
TAMosEsrTesHrsOmDenDtEoVf EthLeOBPoMaEcNouTmuaive
Kopwords
Pro
pe
rt
ie
s
of
Ammoniu
m
Pe
rf
or
oo
ct
an
ate
:
Sa
t
Bioaccumulation; Ammonium perfluorooctancate; fish; static fish test
OntaTyped.
Te
05/31/95
295864 /7288
re
Prove Gofoctve FapartReact
pPerrofcleudourroeosctaarnecadteesc(rPiFbOed)tbhyatthweefraetuhseeaddfmoirnonbotwa,inPiinmgelpahboarlaetsorpyrodmaetlaafso,rftrhoemupditlauktieonawnadtdeerpuursationof ammonium
technique.
These
procedures
were
intended
to
provide
background
information
on
the
ing astatic exposure bioaccumulation of PFO ifnish.
Raper 1Vpe
So v
B0O PRWl&aoDNtR tPcee anns g e anda Devr elocpmehnt 0 Management SUMmary
0DOO) TFERNAPcCTikrayisEpioaordgFinealdnRteport OPO Recover
00JToGEmCohev.nSperrvjiecae:
--_-- O OpenRep andoSur mmatry [EEer > | ss @ ClosedReport - OpenSummary Rear a 0 NowChoris Baporad |
Ifreportisprinted onboth sidesof paper,sendtwo coptioePs& TCS.
:
03713
ASSESSMENTPEORFFLTUHOEROBOICOTAACNCOUAMTUEL:ATISVTEATPIRCOPFEIRSTHIETSESOTF AMMONIUM
ABSTRACT: pPlerarobfcolerudaoutrrooerosyctdaaarnteoaadtefesorcr(itPhbFOee)dubptythaatktehewearnfedathudeseaepddurmafitonirnoonowb,tofaiPanimimmneogpnhailuems pbtraeoccmhkengliraqosuu,en.dfirTnohfmeosdreimlaputritooicnoendounwraettseherweburiseoiancgicnutameunsltdaaettdiicotonepoxrfpoovsPiuFdrOeein fish.
OBJECTIVES: Tddeoevpeualrcoacptomimeopnnltisihnforafispahnr.aellyisTmihisinsaorfyinvifelnsuvtoeirsgitnaietgiabotyniocnionmocbfluusdPteFidOonmu.epttahkoed and
METHODS:
Summary of Practice
Tt(hhteeestuuppttsaaokkleeutpiaohnnadssedweepofruertahtneoitonexrpeteneresiwtemdew)nats,excpfooinssduhurcewteerdseysuteseximpn.ogseadDusrtfiaontrigc13 dtParFyaOsnshfatedorrbdeeidelnuttoiadoddneidlw.uattieroFnoltwloaotwweihrnigcwhhtiahcehsuedplitedackteneodpthcacosonenc,teanitfnriasathdidwoeenrdeofPFO affdiodrsehda,we1wr5aesdeaxyipndoceslpueduderdattowiidotnihluphttahiseoen.twesattA.ercoTnthtoerwohlciocntthrreoaPltFmOepnrhtoa,vdidnieontdwhabiecehn mienadsiucraetioofn otfhetahecceqputaalbiitlyitofy otfhethteesttesftishb,y ganidvintghean shaunidtlaibniglitpryocoefdutrhees.dilution water, food, test conditions, and
wDeurreingremboovtehdpphearseisodiofcaltlhye PFO.
fexrpoemritmeesntt,chafmibsehrsandandwataenralyszaempdlefsor
Apparatus
EcFnoavncidirilotinitmoienenssta-(ltAelLmlapberofaritasuthroert,yesptihsnotwSotep.reerPiacouodln,,duaMcNnt.dedveaPntrtoiptleharetit3oe4ns)t were m(aRiEnVtCOainSecdienwtiitfhictheIncu.s,e Aosfheaviclolnet,roNlCl)e.d environment chamber
1
03714
gCTaeols.tlonRCohccahymelbsietnredsrr,i-caNFlYi)s.hhiaghnddetnesstitysoploutliyoentshylweenree ctaonnktsain(eNdalgien 5
Dilution Water
Slaobuorrcaeto-ryDilfurtoimonanwaotne-sritweasweolbltai(n3e4dWeilnl t#h2e, 3SMt.EnvPaiurlo,nmMeNn)t.al
WcTaortleelaretcmCteoinnotdni-tuisDoiinnlegurt,iaoCncuolwmlamitegerarcniawlaIsntfeciralntrarbtaoitnoi-nofanilltsCeyOrs.et,dempNor(riCtouhrlblrtioogoakn,
hdIoiL)us.rssolvBienedfoorrodexeygruseent,oandbdriilonutgthieotrhnewgpaasHteesranwdiansctoonaceeeqrnuatitreladitbirfoionursmawtofitlheasaitr.12
dCtihhseasroaflcovtleeldroiwozixanytggieocnnh,ar-8a.Cc5atreb&roins0-.t3fiiclsmtge/(LrM;eEdA,cNonadeSur.caDtt.ie)vd:itwyep,lHl,5w180a.t5%er&14h0a.d1; .
Mmhos/cm; 8 mg/L as
alkalinity, caco,.
232
+
8
mg/L
as
CaCO,
hardness,
272
+
Test Material
CGoemnpearnayl, -StC.ommPeaurlc,ialMN,PFOLot(S#p3e7c7i)alwtaysCuhesmeidcaflosr tDhievistieosnt,. 3u
Acute Toxicity determined to be
The 766
mP.g/Lpro(m3eMlaEsnvi9r6ohnomeunrtaLlCsyLafboorratPoFrOy,was
unpublished data).
ucTpoetnsattkaeiCnopinhncagesneatranoftomiiotnnhaesl t- ecsoFtni.csehnAwtnerraeteiaorenlxipeoorfseds2t5utdmoyg/dLi(l3PMuFtOEinonvdiuwrraoitnngmeerntthael Lgcarobenoacrteantteornroayut,gihonuntopduidabllnlioostwheadfdorvdaetrtash)ee,laycicanufdrfiaectcaettemdP.eatsphuraortmeemwlehanistl,eofitthiPwsFOasin test solutions and fish tissues.
SbytodcikssSoollvuitnigonssol-id100P0FOmgi/nLdiPlFuOtisotnocwkatseorl.utions were prepared Test Organism
wSapsecuiseesd-asThethefrteesshtwatoregranfiastmh.ead minnow, Pimephales promelas,
sSuopuprlcye coi.
l-abToerstatofriysh (wAeqrueatiocbtBaiionseydstfermosm, anInce.x,terFnoarlt
bCoilolaisnssa.y
ECnavrieroannmdenHtaanldliLnagbor-atFoirsyh wienre50mgaailnltoaninefdlowat-ththreoug3hu tanks
2
03715
(Frigid Units, dilution water.
IncF.i,sh Towleerdeo,allOHo)wedcontto aiacncilnigmcaatrbon-filtered
dilution testing.
water for at least The concentration
12 of
days before dissol
e to the being used for
holding
tanks
was
maintained
near
ved oxygen in the 100% saturation by
continuous aeration. TetraMin flake food
Fish were (TetraWerke,
fed live brine shrimp Germany). Water
and/or
remained
relatively
constant
at
approximately
14
temperature C.
Age - All fish were 64 days old at the start of the test.
Procedure
Summary Of Test Parameters
* Test temperature: 20 # 1 Cc
* *
Photoperiod: Test solution
16 h:8 h volume:
(light:dark) 15 IL
+ Test solution renewal: mone * Nominal test concentrations (uptake): 0.0 (Control) and
25 ng/x. pro * No.
* No.
replicates per test concentration: organisms per test concentration:
1 30
* *
Length Length
of of
uptake phase: 312 depuration phase:
h (13 360 h
days) (15 days)
* Feeding: fish were fed live bring shrimp
Uptake/DepurationExperiment
Description exposing P.
p-roTmheelausptuankdee/rdepstuartaitciocnonedxipteiroinmsentto idnivlolved
water to which PFO had conducted for a period
been added. of 312 h (13
`The day
uptake
ution phase was
fish been
were transferred to dilution added. This portion o
s). water to
After 312 h, which PFO had
the not
phase,
lasted
for
360
f the experiment, hours (15 days). Fish
the and
depuration test
ppshhoaalssueets,i.onandsapmeprlieosdiwcearlelytaktehnrouatghotuhte beaecghinnofingthaendexpeenrdimoefnetaaclh
cTaersbtonS-efti-lUtper(eUdp,taakee)rat-edTwodiltuetsitontawnaktserw.ereOnfeilled with
with the appropriate volume to produce 15 L of solution
of at
1000 mg/L a nomin
PFO
tank stock
was dosed solution
mg/L PFO. served as
The second a control.
tank contained Dissol
al concentration of 25 only dilution water and
finromtheeacthesttanskoluatnidons.
A
ved oxygen and pH were measured sample of test solution was taken
scintillation
stored at vials (Wheaton
needed for PFO analysis.
4 C in 6 mL polypropylene Scientific, Millville, NJ)
until
aTdhdiirttiyonPa.l prfoimshelwaesrewerseacraidfdiecdedtoanedachtheoifr twheet tweesitghttasnksw.ere Ten
?
03716
determined in order ten fish was placed
to in
calculate loading rate. a separate 15 mL polyp
Each of the
Cceunnttriilfuagnealtyuzbeed (fCoorrnbiancgkgIrnco.u,ndCoPrFnOicnogn,ceNnYt)raatnridoopnys.lteorneed at -25
Test Set-Up experiment,
(Depuration) remaining fish
Following the uptake phase were transferred to tw
of
the
containing only 15 L Dissolved oxygen and
carbon-filtered, PH were measured
aerated in
o test tanks dilution water.
transfer of fish, each tank for PFO
and test solution analysis.
sampleesacwherteanktafkoelnlofwrionmg
Test Maintenance tanks were taken
- Dissolved OXygen measurements daily during both phases of the
in the test
test
drfeiemlslasionlbdveeelrdowoofx6.y0gtehmeng/ctLeo,sntc.beontthFriastthiaonwknesriewnerefeeidthgeelrnitveloyfbartiehnreatteedst f.otranIktfshethe
72, 120, 168, after starti
240,
288,
336,
384,
432,
480,
e shrimp 24,
528 and 576 h
removed
ng the from tanks
test. with a
Fecal material/excess food was disposable glass pipette asneeded.
Test solution pH was experimental phase.
measured
once
more
at
the
end
of
each
Test Sampling - Five and 312 h during the
fish were removed from uptake phase and again
each tank 24, 96,
at
192
w3e6r0ehsaacfrtiefricbeedingby tfrraeneszfienrgredintoliqculiedannidtirlution
168 water.
and Fish
allowed
to
thaw,
and wet weights were
ogen. Fish were determined. Each fish
was and
placed stored
in at
a separate -25 C until
15 mL polypropylene centrifuge needed for analysis. Test
tube
msLamppolleysprwoepryeletnaekenscaitntiealclhatifoinshvisaalmspliatng4 tCimuentainld nseteodrseeoddlutfioniron6
analysis.
Analysis of PFO
F- ion. Whole fish and
organic halide
atnesatlyzseorlut(iDoonhsrmwaenrneDXc-o2m0b0u0s,tedRosienmoaunmtodified
Analytical collection
Inc., fluid
Santa Clara, was measured
CA), with
and F~ activity in an ion-selective elect
connected MA). The
to an ion analyzer (Orion Research concentration of PFO in whole fish
Inc.,
rode Boston,
solutions
was
calculated
from
the
measured
tissue and test concentrations of
Data Analysis
Ac0.to0m5pe)aa.rcehd staompltihnatg pofoincto,ntrtohle wFeiisghhtusiofng PaFOt--erxopeocsed(asfsihsnh was
O0.n0e5-)wayweraenaluysseids toofcovmaprairaencetheandavTeurakgeey'scoHnSceDnttreasttio(naslpohfa P= FO
*
03717
dienputriastsuieosn efxrpoemriPmFeOn-te.xposed fish during the uptake and
RESULTS: Test Organism Loading Rate wTeh.e) lpoeardinlgiterrateofoftesPt. psorloumteiloans. was 124.6 * 20 mg ish (wet Water Quality Characteristics tMlheeeaveslcusornetddruorwliantgaenrdthqeuPaFsOltiattteyisctparseoaxlmpueerttiiemorensnstr.weamsaTihn8e.e2daw%viet0r.ha2ignefpaorHccoetfphetbaobtlhe cteohnnettirrtoeelstt.essotl.TuhteirToenhfeodrrdeoi,pspsaeoedlvrbeaedtliooownxyog6fe.0ntmhcego/nLtcees7nt2trhsaotlaiufottnieoronfssttawharesting itinhneibtoietxahpteerdtiemsaettnts7.o2luh,tWiiotanhnsdaercreoamntatiiionnneu,dedatthtehorroduingsehsaoourltve1d0t0h%oexysrgaeetmunarianltdeievoernl.sof Concentration Of PFO In Test Solutions pdThuharesienc,goncttehhenetmrseatatastiuiocrnedoefxcpooPnsFcuOerneitnreaxttpeiesortinmseoonfltu.tPiFOonDusirnirnsegpmiaiktnehededudpictlouantksietoannt cwaoalnltceerontthwreaarstitcoeonsnstsiwssteoerlneuttiaolanwtsa,yaspdpulrreosixsnigmatethiaentlhye1r.205phmmaggs//eLL,. (PTFaOble 1). In Survival And Growth Of Test Fish tJR.heelparetoximpveeerliatmsoenetthx.eposcTeohdnetrtpooelrPcFteOrnetwatemrseeunrtvn,iovtatlahdevofesrusrtehveliyvcaolanftfarenocdltegdriosdwhutrhiannogdf datidhddeitnPioFotOn-,deixfptfoheseredasviefgriansgihefiwcweaerntetlw9ye7igafhrntodm o93ft,hePrFaeOvs-epeerxcaptgoiesveewdleytP.. wperiIgonhmtelaofs c1o.ntrol fish at any of the sampling points (Table 2, igure Concentration Of PFO In Test Fish Uptake and Depuration bTrheeegliantcneoidnncgetnotofrtahtetiheocnounpocfteanktPerFOapthimaoesnaes,uofretdhPeFOinPFiOnficstohenstcteinsstoslruuaettiiwooanns. diniArtefcitstlhhye tissue was < 1.0 ug/g (wet wt.). After 192 and 312 h of
5
c03718
rOSfexsppoPesFOcutrieivnetlofyisPhF(OTtabiinlsesdu3ei,luwtFasiigoun4r4e.w7at2e)a.rn,d
4t6h.e7 aPvge/rgage(wectonwceen.t)ration The concentration of pro
seiaxnmpopfsliuisrnheg,ditdbiumtensowtatshdainfs.fiaegtrni0fsiihcg.annftil2c4yanhtglryaefattbeeerrtwbeaeteinnbgo1t92htraoafnnsdfte3hr3er3seedbn
of to
tCchlheeaaccneonnwtacrteaentrti,roantPioFofOn-e1o9xf.p9oPsFueOgd/gifnis(PhwFetOh-adwetx.pa)on.seadveAtrfaigs96eh approxima
hPFpOosits-aenxpgosure, had decrenced. co
cgoinmesta=nt672utnethl)iy.l t`ejTsght/egctoe(nrwcmeetinnwtatrt.ai)ot,nionaatnodf36r0PeFmOhaini(encdufmiruselhlaattitviiesvseulceyistct
fEornomet8h,a2t7dmea3s6u0rehdpaotst-teesxtpossutraretdi(d0 nh)o.t differ significantly
Bioconcentration Factor
bTPihroeocoirnnecleatnthteiroanttsiihsoisnupefboaefcttwoeP.renp(reCoxFmp)eo.lsaurseBwiaotsocodnPecFseOcnrtairnbadetditohnbeyraeamfoeurnst the increa
of to
TphaessiBnCgF wdaisrseecdteolffiynaeidnptoaolsl:uatnanatquactoinccensptercaiteison (fMroormiawrattyer198w3h)e.m
BCF = Cu/C,
pawohqleurlaeut,tiacnCt,or=gianneiqwsuamit,leirbarntiodumwCyhci=ocnhecqetunhitelriaobtrrigioaunnmiosfcmonaicsepnoetlxrlpauottsiaeondnt.
oifn 4the Because
tithhdaet ncaoontncacephnpatanrragetenitobnesetqwueoieflniPb1Fr9Oi2uiman,ndtoer3s1t2stsheo,alduytthiesotnaatsaesn,dumhpfatidisohbnetweianmsesmade
cprareloacmcuehlleaadtseid3n12atshhethasetpaptcaiorcenncteenxtsprtoaestuairdoeyn-sstoyfastteePmF.OBCEinTh(efBriCesFfhioyrt)ei,ssfuoterhePaFtbpO. 3w1a2s
hh, diorvided by the concentration of PFO in test sol=ution ac 3i2
BCom = 46.7 Ug/g (wet we.)/25.9 pg/mL = 1.8.
REFERENCES:
Moriaritny,EcFo.sys1t9e8m3.s. Pp. 233.
EcAoctaodxeimciocloPgrye:ss, ThIenc.S,tudOyrlaonfdoP,ollFuL,tan2t3s3
6
03719
FIGS 1. SaavmeDr1aegde wtehtrowuegihoguhttsUopftaCornet/roDlepgerioouorpn ranodriBaRmG-nesx"posed . pronelas
80
:
= 70
Uptake
2 P E 60 pr
2=
5 AS 3 50
E 3
BO -- profisn
i
|ii
pg
L] y
] ;
-- Control fish i
2 30 0
100
200
:
300
400
g
8
Time (h)
aN Depuration
. IN N \.
\
g
500 600 700
FIGIE 2. Uptake and depuration of BObyb. pronetas
60
y
350
:
2
Uptake
4
40
=
]
i
= 30
!
Depuration
= 20
3 10
o
.3
0
3
PR
:; v
5x
] 100 200 300 400 500 600 700
Time (h)
TABLE 1. Csoonlucteinotnrsa.tion of PFO measured in test
SoPlhuastei;on | TimPehase(h)*
Avg. PFO |conc. (ug/mn)
upTAKE
Control
0
<1
192
<1
-
312
<1
-
25 pgP/FmOi (N)
0 192
25.0 25.5
1.8 2.2
312
25.9
1.8
DEPURATION
control
0
<1
168
<1
-
360
0 168
<1 <1
-
*
Elapsed
time
(h)
from
360 the beginning
of
each
* Test solution containing pro-exposed fish.
sxperinental
phase.
GO3722
TABLE 2. PA.veprraogmeelwaest. weights of Control group and PFO-Exposed
Time (h) Average Wet We. (g)|
Std. Dev.
Average |wet wt. (q)|
std. pev.
0 192 312
61.7 61.4
5.2 20.8
61.7 57.8
5.2 13.4
55.3
11.7
51.5
336 (24)+ 408 (96)
49.4 66.4
13.9 7.3
55.5 65.8
42.0 22.4 22.4
480 (168) 672 (360 umber in ()
73.2 32.7 indicates elapsed
44a.a 16.8 time (h) from
74.6 beginn5in3g.6of
55.8 depuration16p.a9ve:
Results sampled
of at
tth-etesstame(2txi0m.e0.5)
comparing
wet
weights
of
control
and
PFO-exposed
fish
Not Significant.
EXT n.s. n.s. n.s. ns. n.s. n.s.
03723
TABLE 3.
Average concentration (measured as F-) in
of
PFO
P. promelas tissues.
Time (h)
PFO (ug/g,
In
Fish std.
Comparison] of
0
wet we.)
Dev.
1.7
0.4
192 312
44.7 46.7
9.1 5.8
[2T1 aT TT
336 (24) 19.9
408 (96)
7.6
5.1 T= 1.3 -- Tc]
*
480
672
(168)
(360.
Munber in ()
7.6
8.0 indicates elapsed
3.5
time 1(.h6)
1 Tc]
fr| om beg|inT ing c of
+dMeepaunrsatiwoitnhphtahsee.same letter are not significantly different
4t a1pha=0.05 by Ditference test.
the
Tukey-Kramer
Honest
Significant
03724
Appendix Comments from Dr. John Giesy, Michigan State University
03725
March 20, 1995 Dr. Robert Howell
E3n-vMirCoonr.pL.ab. Building 2-3E-09 P.0.Box3331
St. Paul, MN 55113
Dear Robert: 1Pehraflvuecrroeovciteawneoadtteh:eSrteaptiocrFtiesnhtTitelsetd,Awshsiecshspmreenptaorfetdbhye BJiaomaecscDurmauklea.tivIfeoPurnodpenrotfiaetsaolfeArrmomrosinnituhme experimental design, methods,datainterpretationorconclusionsdrawfromthis study. Tahnadvseuagtgteascthieodnsasfhoorrfturteuproersttwuhdiiecsh.containsmyspecificcommentsonthestudythat wasconducted. Please contact meifyou have additional questions.
Sincerely, John P. Giesy, Ph.D.
enc: Report
C03726
Report onpigfberiment:
Bioaccumalative Propertiesof A `which was pre
Yerfluorooctanoate: Static Fish Test, James Drake.
Soha ERR, PD. Principi@yentist
Giesy Ecqtaxindlogy, Inc.
Marksed1995
Dr. RofiesLtfHowell
34]
Environ. Law, Bullling 2-3E-09
. P.OIBax331
St. PagRe@EN 55113
Comments:
General:
1) Tdheesisgent,omfee thoxds p seleecwtear ds,wsei taltlim sdteisce sigarpnifsBEthdgTMis @ithecroenacrluesnioonfsatdarlaewrnr.orsinlog=ice, experimental
2) Thereportiswellwritten and theresideclaftlypresented.
:
=
1
:
co3727
3) Tagree with the conclusions that there is little bio
BEST COPY4 VAll4p
by the fathead minnow,
accumulationofperfluorooctanoate (FO) LE
Specific:
1) Teuhsxeepfeuurlsiiemnedoneftmtohdneosfetasrtahnetoaitdngamditdnhranetostswhetarhseeaiismsolsdiuegebg-sMoabPeitsgiceusmuilnaitniitoanlviepaxotpfeeonrtiimaelnotfs PisEGap,pr`oHporwieavteera,ntdhiiss
predatory
PF to l
fishes.
Some
consideration
sileghkummualdaetoifofneedingofda,thbeyadtshiscosnpteacmiiensaorreldarsgesr
O arger fishes ina longer expose"
2) Tuchsoeenfceuexlnpttorosauctroieonpndeu(rcSitSoCtdhs)eefeermxopsmotsoeuxbrteeeraantadl eesqEueaYrHgoplwdienvttgheaparte,trbihnelfiui(stuhsrheahdeaxtpteariinmeedntasstitewaoduylstdabtee
`more definitively that SSC had fr
iod 4-5 days) longer to establish
|
3 itTshhpirseovrbaaelbmuleuysienovdfeitcnhaleteesssst.tuhdIayttihtnidhniekcrtaehtaeisttlhmiaottlstethedtiaiptagiiom0untlaOtfiPonFOfa,cttohreitsraueppbriooxaicmcautmeullyat1.i8o,n fWahcitloer
rPfroepssu.ernftaTecwedo,suotlrhpdetistourng.ugeeTBshCutsEh,atitshiepnrsaotnbuadfbylpyra&8B852i8u@@yereeishgthaittmaw8tea)ssotahrcerctiurnmueuel(B9a)CteElde.bssyBttahhsaeendf-tihosenhtwrhaeepsorddtaueteda
Hours clean Sowingwtater beforyethuetyu8r3esdthantttohetfhieshcbleearniwnasetderfotroafpplrooxwidmeaptuerlaytifoonur
thWhyhedirBloeCcaFrtbheoexnpsae,ccctituemidsunflooarttuidnoeetnexrppgeoetcnettnset.di,albaSsEIaNbtltahteivsetruscmtaulrle, orfetlhaeticveomtpoouontdh,eranhdalsotgeangateessod
aTchceumcuulrraetnitonstoufdyPFaOd)drinetsosetdheofnilsyheasvsiihfugehgwaaossunrotercaopnicdelnytdreaptiuorna.tedThiendfiaccattetshatthtthetrhoerweafsy db`isyadcotnc,huotmtiusulseateiinmodtnicoianittneocraheatatisgehbdtaulsryeibdnooguntnhdae paphxoaponkGeJRiKcnrdetitciacttaheastssettshhesamtcteohnntictsehpnaottrotalthhieoanBdOCfbFePccoFouOmledisnbatethubaritaaptsoeedod.l
{Sfooumnwdehpiocsoheltoshfaruessigneglgteeh,mae1trste0slhaebttreievenaloryedgetrpweuoraagniitieoibpgiRlraaeolncodcnuocrmeipnnotgrnatethnietosdn,u.raaTnthieoevoeerxfyitsrthaepnicsdetoufantdhe1stigehtely et0so,ittahietnntiehnxeptsohaseumrweeamttaeorrliewsmosaeurrlbdcoobnuecngedrnetPraEatGtSigFnPEfiBsuEaR,tFhlOethfBeCoFql,uonwgheirchpeirs itohdesronaftiiomoef PwoFuOdlyi.dnptohsessfiibslhy ccoonnssitdaenrtesdtirnutehceopnrsetsaenntts,stwudhyi.cFhoarrienisdtretf_ngcfaRddBetnhte offisehxwpeeosrsteuiorenexpcisoo,nsceaerdnettrtoahtaeicoponh.nacreTmnhtaircsaotkwiiaonnseotnniocet
`hundredth as great for a longerperiodBERME but still reached the same total body burden,
fetal
2
co728
tadhseepgeBrneCdaEat.nfcFoerutoPunFredOusratacutcdiuiomensulasanhtdoiuiolnntdebniysnitcthlyouedffeeaxtahpneoaasdsusrmeeis.nsnmoenwts owfoutlhed pbheaormnaechou-knidnreetdic(1c0o0n)sttiamneesy 4) cTdoenhpceeunrdtaurtraaittoiinoonwno(af5stmughn/elkikdgee,lpywu.wra)Tthitoehnaapschecaeusmmeeywdltaasotbiaeodneteqiaugphafsttlmeyubtocoeuhsntadasibln5iosphrptohmnatitshnetohfetisfahisashmnahdlatldhaartmeofauucrnhtehdear
ccoonmcpeonturnadt,iionfst.heTheisxproessuurlet rcaoinscesentthr@gWie@sithailben aotfawllhreerleevtahnet PfoFrOexipseacstseodcieantveidrownimtehntttaohlef aorregatnhiesmt.arIgseittssfuorrfaaccec-ubmouulnadtoiornb? oundjgitisisuetsoeftmheafislh? If so, whichtissues
5) WPshtFiuOdlieietssahnneodetxacpcoeutrneilmsyetinotswxwiaticsttehonntfohttaetcmhoeeadndidarudictggoMRlgiozaBmbs8iceTeshlsiimstiiotxsiLscCiim-tiy5loa0rfoPtfFooOrve,esrutlh7te0sr0weismutglh/tsLP.iFnOdiicnateotthhaetr Tp`rwhooeluilfdemreanctoihtoanbn,ieissemxapmeoocfrteeadcchtiirnaoonnisochfeofrpfteetrceftlpiaddtuamnaf8a.ddvTcehorumsspe,oetufhfneedcsct,hsrodwnuhieictchthiosximisceicothyftaoenfnipPseFmrOoofxtaiocstfoiimsohen, egmsiprenocnwiotawlhlsy.apnrdedraetporroyduficsthieod,n rweemraeinnsoatnaSoc@dyf@e8astt#ioinn.thPeotbeinotaicacluemfuflecattsioonn lsotnugd-ytewrimtshufravtihveaald,
7) T`whaesunsoedoefgraasdtaattiicosnyosftePmFsOeeamnsduasctofnestgathitexhpBoctsuuarlecocnocnencternattriaotnisownewraesmmeaaisntuariendeda.ndFutthuerree stisudtiheespwrietfehrsrmeadllmeertchoondcoelnotrgaytiinodnesfsinhiogvleSgeeBsidotfehbreiouascecuomufalaftlioown-.through system, which
Potentialfuture studies: 1) `DorigansismpaouotfoPrsaFdOiioignrtfaipshihyt.iossunes. IwEBaraW dio-trai cerstg udyfoe lloweds bywht ole2) TtchhueerrrfeeancctotutlshdtaubtdteyhceoronensslieydeermaadsbdtlreoaebscseceuidmurbliarfoBeieugSgvelsgaeetciigaolnlioy fffrrPoogFmmOitihwnaefttdeoirtie.htedoffapitArheendadadigtseot,raiyrnydfiicssahtetuse.dsytThhaoetr
biomagnification study is indicated. 3) Cschthrrroaoninigichcetef-ffffeoecrctwtssaroodfenxsstpuuordvsyiuvtraole,ctgoonrPdoFuwctOthoananrEigfzg@euciaamdmeadtrioyoi.vneonffeaetdehdeaidnfmoirnmnaotwiso.n onThtihseipsaorteelnatitailvefloyr
ie
3
0379
4 Tf`eThehedepipnootgsentttuideaylfwnofiotrlthloponingrg-a-tetederlmramb,tcfoihsrirhoenysioceffmectsawaangsdenfofrtecatdsidcroeufslsdebdienatschsiessassteudidtynorilaonnygooetrthoennrsg,,
ee
4
.
05730
PFOA and Associated Salts
S12y6n,oNn-y2m8s0:3-F3C,-F2X6-,1F0X0-31,00F1C,-1P1F8,O,FCF-C1-011453,, 7F6C.-0130,1F5C--X
PhysicallChomical Proportios
TEAutE hor pi
[Water.acetone and toluene solubitty estim-aFCt-e14s3| 3m Env. Lab | 11/17/81 _[|Rcoopybusotf sdautmamasrheye,t Environmental Fate and Transport
[Ready Bodegradationof Fo:143 (8O0IGOBITOGT S[oTielc,haincadlaFropuorteSfruommOr. m-.AdaBsaoyrrep,lyMoenhoiTgFaGn S08ataendUrFiCvr1s4t3y [TechicalReportSummary ~ BiodegradStautdiieosonf
WAEut.horLab
|
Dato 7477
[Robust summary,
copy oftaboratory
D277, [gaRtoabsusutmsmuamrmayry,
5/1993 tlercthinqiuceablyreSp.orBto,yd|
TITSTE [RMoSbuUs)t summary,
my, WE |Te [Technical Report Summary- FG-123 Proialyels Siody Using | SM Agrihemeal
[Robust summary,
re [REE| [[BTeecghnicFailshR,epaort aSnrumtmdaery-bAyJn.alWy.siGs Toir iollfoCeroamactihleUmniicvanesrs
S8T9G3, f[rReocbhunsitcaslurmepmoarrt,y,
critiquebyJ. Gillet]
[Ready Biodegradation of F123 (80G7G00]
RCoobrusetnsummary,
|copiesofdata
[Caleuatod oetanarwaterpartioncosficent ~FG-143
TT [Rsohebeutss.t summary,
loopy of
calculations
[Robust summary,
computer
[greepnoerrta,tceodpfiiensalof
[Ready biodegraodfaFXt-i10o0n1 (BODICOD)
MEnv.Lab | 27685 raw data sheets.
[Robust summary.
computer
[Ready biodegradationof Fc-126 (B0DICOD)
Pace Analytical,
lrgeeponretr,actoedpyinoafl
MinneapoliMsN,| 5/20/87 finalreport
i
03731