Document gjgObDO7eQzdYQEB4pgdxM3a
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AR 226 -- 0825
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BIODEGRADATION (BOD/COD)
IESTSUBSTANCE
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,12,2,3,3,4.4,6,5,6,6.7,7.8,8.8heptadecafluoro-, potassium salt, CAS# 2795-39-3)
Remarks: The 3M production lot number was not noted. The test sample is FC-600. Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % tolyltriazole. The following is an abbreviated summary of multiple biodegradation istnucdoimepsleotnelFyC-c6h0a0r.actTerhiezseedsctoundcieenstaraptpiloyntoofaimmipuxrtiutrieesw.itDhata does not accurately reflect degradation potential of the fluorochemical component of the test sample.
M0 ETHO0 D: 00000000000
Methods: OECD 301E and BOD/COD/TOC (summary listing only)
Type: Aerobic
GLP: No
Year completed: 1975, 1963, 1984, 1988
Contact time: 20 days (BOD) and 28 days (OECD 301E)
Inoculum: 1984 study used a 50/50 mixture of soil extract and
secondary effluent. Sandy loam sails obtained from Ramsey and
Washington counties in Minnesota. The secondary effluent sample was
supematant of activated sludge mixed liquor taken from the Metro
Wastewater Treatment Plant, St. Paul, MN. Inoculumforthe other studies.
was not noted.
-
R0 ESO0 WTS 00000000000
Chemical Oxygen Demand Biochemical Oxygen Demand (BOD):
467,000 m/L*
5Day: 50,000 mglL*
11Day: 255000 mgiL*
_.
14Day: 240,000 mglL*
20-Day: 237,000 mgiL*
Total Organic*CDaartbaonwa(scaalvcauillaatbeldfeorasthaespurmomduacrty):list on0ly..097 gig
001951
Total Organic Carbon Removal:
90-91% in 28 days
Dissolved Organic Carbon Removal: 79.9 -- 94% in 28 days" ** Range over 3 separate OECD 301E studies
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS
No reliable conclusions can be derived from this study. The indication is that the majoroiftthye product components are readily biodegradable. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
D0 ATA0 QUAL0 TY 0000000000
Reliability: Kiimisch ranking 3. The inoculum is not properly characterized in most studies. The testing was conducted on a mixture with chemicals having varying degrees of biodegradability, leading to potential misinterpretation of the results. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solutions.
REFERENCES
~~~
00000000000
Testing was conducted by the 3M Company, 3M Environmental Lab, St. Paul, MN, Lab Request Numbers 2874 and 8483.
3M Environment Assessment inquiry #32326, E. A. Reiner, 3M Environmental Laboratory, St. Paul, MN, April 21, 1983
3M Technical Report "OECD Biodegradation Testing on FC-600 and FC- 203." E. A. Reiner, Project 9972410100, 3M Germany, Report Number 001,April 18, 1983
3M Technical Report "Modified OECD Screening Test on FC-203, FC203A, and FC-600." E. A. Reiner, Project 9970010100, Intemational, Report Number 003, July 9, 1984.
_
Last changed: 6/28/00
001952
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Form 6747118
TECHNICAL REPORT SUMMARY
TO: TECHNICAL COMMUNICATIONS CENTER -- 201.2012 {important -- Ifraport printed on both siodfpaepesr,sendtwo copies to TCC.) Guidelinoens rovers side.
3M Environmental Laboratory (EE & PC) St. Paul Minnesota
0535
3M _Gernan
997241010
?OECD Biodegradation Testing on FC-600 and FC-203 TKr. aFp. SchrFroeeedser, 34 Neuss, German:
c. a4Biiner, Environmental Laboratory - 21-24-05
FSOco at4t/r18/N8a3ma
47816
None - See 3M Environmental Lab Request 8483
15
Sey GornorRocart SummauryryC0IERhpir=ipmnmpsubmmary| ABACE H D enifFoerwcmhesicoatn arorseeas ped s Un
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[CURRENT GRIECTIVE
p1.roduToctsmeaussuirneg tthhee biinotdeergnraatdiaonbaillliytyroefcogtnwioze"dLigMhotdifWiaetedr"
OtherKeywords
OECD Screening Test.
2. To compare results with those previously obtained using the same test method.
E[oRmEoPaOrRyT AAGRSGT.AAGT: Thi osactnfrmaion GourdBY th Technical Communications Corer 1 ars 3s
dThiisssolsvteuddyorugsaendicthecarMboodnifi(eDdOC)OECaDnalSycsriesenitnogmeTaessutrewitthhe
FbCi-o2d0e3graanddabiFlCi-t6y00.of I"nLig28htdaWyast,er"theBraDnOdC AlFeFvFelsproofducFtCs-:600
pdaergarlaldeeld tbyest8s0.% anIdn 9272%dafyosr tanheavDOeCragoef OFfC-28063% diengrtawdoed by
77% and 81% for differed from a
sainmialvaerragteestofdo7n9e%, inTh1e980FC-in203whircehsul9t3%
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001964
PFaCg-e6002 /FC-203 april 18, 1983
Introduction
obTfhieodceo3gMnrcaeEdrnanvbiireloxinptmryeenstsoaefld "bLLyiagbho3tratWGoaertreymra"nuyndperarobdtououcottksi,tnhciFosCn-s6is0ts0utdeynacndiofesPCt-hi2on03p,astbucause
biodegradation test results.
MhEoindgvihiflriyoendmbeinOotEdaCelDgraSLdcaarbbeolerena.itonrgyFTCe-ss2ht0o3swAe,dconFtdhCua-ct2t06et,dhreaiennd 1"9FL8iC0g-h3t0b1y7Watth(eeFrC"3-u20p6rodF)uctasllwere
wtehreoOESChDownScrteoenhianvge T9e0%st.of tThheeisre driesssulotlsv,edhoowrsgvaenri,c ccaornbtornadircetmovtehdosoinof
mHF.eCt-h3Ho0ed1l,7lmawtnehnree TaOnfCdounDD.degMrtuoaldlahetarvi(eoln)tToetusaslti(n2go)r.gaansiiIcmnilctaahrrebiorynetstrunedodtyuctFiiCdo-en2ns0t6icoafalndontleyst 6%
BHaneOdlDlsm/8a%Cn,OnD,rreashtpoiewoecvsteirvf,eolryw,eFrCe-i3n 0a1l271sodaancydosn.tFrCa-d2Ti0h6cetseewderleobyw 0h.bi5isodaeonwdgnra0d.wa4o,trki.ornespHrieescstuilvteslyo.f
oThxipsresssheodwedwitthhiant Greater than the
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dJiosncgerrepa(n2c1ydaiyn) hiTsOCwodrekg.radation test. This is clearly an inexplicable
mHIeenaldsleumpraeennmnde'ensnttsTOlCmaabddoeergarbtyaodraittehiseono3nM reE34snuvliAtrFsoFnFmaerpnertoadalulcstoLsa.bcoornatStruoacrdhyicrtaeensddultobstyheBtryOpDi2c/aClOlDy
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AFFF products made by 3M is shown in Table 1.
001965
Page 3
TABLE 1
BOD, CARBONACEOUS BOD,
Lab
(BnOgD/2kgg)
IM
Pace Csearpcsoule SG$al&brWaiStehed GKanlobxrvaiiltlhe
500,000
800,000%. 454700,,000000 616,000 670,000
AND COD DATA ON MIL-SPEC AKL (cmg-/Bkogp) (mgc/okpg) CBOODDg FC-203C Lot 501 600,000 774300,,000000 .79 730,000% * 871300,,000000 464200,,000000 973570,,000000 ..660 671,000 '. 776,450 .79 641,000 776,450 .06
PRODUC "c-eBooDn,"g
81
+"6605 `86 82
A5.verDa. ge
58755,,000000 59904,,000000 78820,,000000 ..7i32
1725
* CianlccounlsaitsetdentB.OD'sPacferomagrdeiefdferteontredpielauttitohniss wweorrek vbeurty aiindadvneotrternetqluyestlosatthtihredirtreespteiantgednortedsitd rweesulitnscluadsewetlhle.se We values in the averages.
[FC-L2o0t 560C2
Mm
290,000 290,000 338600,,000000 0.78
0.78
PSaecreco
325400,,000000 327600,,000000 435700,,000000 00..7685
00..8720
Gcaaplsburlaeith
421660,,500000 342101,,000000 441007,,020500 01..6032
01.0718
S & W Seed
GKanlobxrvaiiltlhe
395,500 374,000 407,250 .97
02
Average 8. D.
m SPaecreco Capsule
325,000 338,000 396,000 .81 73,000 "57,00 33,000 116
FC-780B Lot 501 240,000 230,000 320,000 .74 250,000 250,000 333500,,000000 .71 224100,,000000 223200,,000000 326807,,000000 ..6773
8 a1
Ba a771 64
A8.verDa.ge
23157,,000000 23"123,,000000 32298,,000000 ..0731
2108
001966
FPaCg-e6040/FC-203 April 18, 1983
Methods and Materials 1. Procedure
rteessttiinngg wofaschpeemrifcoarlmsedadionptaecdcorMdayanc1e2, wi1t9h81.the OCD Guideline: tor 2. Chemicals
Ti(hlCeyuHfsreNedaf0esrf)oncF1e..W0.malt=ietre1ir4as4l.1w1iu,tshedAtwhae3s0.n3ultamrbgioernasttaomrpsyloelugtorfaidoenthidsseosdcicuromimbpeohduenndzboyawtateshe mSeatmhpoldestousyedielwderea stuhpepolrieetdicably GD.OCPoifess2e0nsm,g/lE..T.ST.hLe. "aLnidghtcoWnastiesrt"ed oSfoluFtCi-o2n0s3 aOfnd tFhCe-6"0L0i.ght LoWtatenru"mbeprrsodwuecrtes nwoitthprDoOvCi'dsedo.f Nutrient waeprperoxpirmeaptaerleyd h20y mbgr/ilngi(nbgasehdothon5c27alcmgulaoftioFnCs-60f0romandthe153fomrgmulofation) C-203 to 2.0 liters with nutrient solution. 3. Inoculum eThfefluiennotculiunmocuwlaas eaac5h0/5p0rempiaxretdurein ofacctoherdasnociel waintdh stehceondOaErCyD opbrtoaciednuerde,fromTheWhistoeilBeuasredLwakaes, fMriensnheslootaam,y asnadndthgeardweantersoiflor the 5e0fif1lueenxttrascatmipolne wwaass cthhelorsiunpee-rfnraeteantwelflrowmataenr.actiTvheatesdecosnlduadrgye aTheeractoimobninbeadsininaotcultuhem wSat.s PpaauslsedMettrhorouWgahsteWwhaattemranTre5a4tmfeinltterPlapnatp.er prior to usage. 4. sterile Controls Rcoenptlrioclaste fovressbeoltsh cteosnttaimnaitnegria4l0s0 magnd/l tHhegClrpefesreernvceed caosmpsotuenrdi.le The custletruirlees.controls were handled in the same way as the viable 5. Instrument Tsheensiotrigvaintiyc lciamribton isanaalpypzreorximuasteedlywas2 magDoofhrmcaarnbnonDCp-e52rA.liteIrt.s 6. Sample Handling and Storage mTHhaCenLufpsaeacrmtpul1re0ermp.lresofTehrevfaimtleittoehnroeddmetsihanomvdpollevu.esded Tahdwidassingbthrao1 tugdhrpotrpestochferibcseoadnmcpbelynetstrhatetoedTOC linessviatlhsanwipHth2.aluTmhienumsamfpoliels-lwienreed tcahpesn. stoDroehdrmaunnnderlirteefrartiugreeration pihntdhiaclaattees, thaatretahdeilymetbhiooddegcarnadasbtlaebilmiazteeripaolt,assfiourmsheyvderraolgenmonths.
001967
ett Ak Ate re FRTr te i
FPCag-e6005/FC-203 April 18, 1983
hDuaelf toof prtoheblesmasmplweisthwetrhee asneanlsyizteidvitwyithoifn tahemoTnOtChanaafltyezrers,ampolnilnyg. Froelalnoawliyznegd.4-5Cmoomnptahrsisoonf osftortahgee,TOCallanaoflystehse dsoanmeplewsithwionre 1 month owniethsttohroesde sdoanmepleaftheadr t4h-e5 TmOoCnthcshanogfedstosriaggneifischaonwteldy.thatThiisn sonalmyple was discarded. Results and Discussion cTaobnlteain2 stuhemmaarcituzaels dtahtea rsehseuelttss oafnd tphlisotsstuodfy.theAdpepgernaddiacteison1 aansda 2 cfoumnpcotuinodn woafs tinmeea.rlyTacbolmeple2teslhyowsdegtrhaadtedthewitshoidnium14bdeanyzso.ateDergerfaedreantcieon O2f8-dbaoythteFsCt-60p0eriaondd wFiCt-h2o0u3tcopnltaticnauueidngt.hroAutgho2u8tdatyhse ctoheursaeverofagetheT0C FrCe-m2o0v3alwafsor79F%C.-600Thewastes8t6%praondcedautre27 rdeacyosmmetnhdesavtehraatgecheTmOiCcarlesmovgailvinfgora rreesgualrtdedgreasaterreadtihlayn 7b0i%odelgorsasdaobfleD.OC T(hweistehin3428fodramyusl)atesdhoAulFdFFbeproducts carheemitchauls teovenbemocroenscidoemrpeldeterleyadidleygrabdieoddegtrhaadnabilse.necessary for a
TABLE 2 Biodegradation (8 DOCoSremaondvalFe)ma06of Sodium Benzoeateee
Product
7
1 Degr2adation 2a7t Day: 28
SSooddiiuumm BBeennzzooaattee Duplicate 9924..34 9964..37 9993..62 9973..75 10904..05
FFCC--220033 Duplicate
4337..04 5549..62 6668..04 8716..37 7* 8.0
FFCC--660000 Duplicate
4467.2 6673..15 8703..25 8882..71 7992..91
*Sample discarded due to probable contamination.
eThveidesntceeriltehatconstorloulbledatTaOC inlosTsablferom3 ptrhoevitdeest fsuarmtphleers suisbstnaotntidaule to pThheysseicaslampmloedseswesruechhaasndlaeddsoripdteinotni,calvloylattiolitzhaetitoens,t voerssperlesc,ipietxacteipotn. 2th8a-tdaythetyestconpetraiionde,d tHhgeCsle, ctoontprroelvsentconmtiacirnoebdialallgroowftht.he AitnittihealenDdOC.of
001968
FPaCg-o6006/FC-203 April 18, 1983
Percent of Initial DOC in Sterile Controls at 2u days
Product
$ DUC Remaining
Fe-203
98.1
.. C-600
101.3
Conclusion
.
TFhCe-60p0resaerent"rsetauddiylydemboinosdtergartaedsablteh"atacLciogrhdtinWgatetro ptrheodurcetcsommFeCn-d2e0d3 a7n0d% FDOCC-20r6emohvaadlavcerriatgeerialoosfsestheof MoDOdCifiofed79O%ECDanSdcr8e6%e,ninrgesTpeesctt.ivelFyC.-203 and References
1. EDrx.tinH.guiHsehlilnmgannFoaamndCoD.mpoMuunldlser,(Um"wEenlvitrgoenfmaehnrtdaulngIdmupraccht of Fire
GFeerumearnlosIcnhs-tiStcuhtaeummofittHeyldlr)o.l"ogy Th(eBusntduedsyanwsatsaltcarfruiredGewoaustsebrykutnhdee)W,est
April 18, 1979.
-
2. tD.o MTeusltlerOrgaanndicT. MaTtietrtiiazelrs, fo"rThCeomTp0lCetDeegBriaoddaetgiroandaTteisotn- (ADerProTocCessAabufbauVtoelsltsta-ndeiignenVerBfiaohlroegniaczhuernPArubfbuanug)r" vonDeOvreglaonpbeidschaecnthSeubstaenzeetn ABbuwnadsessearn-sFtoarlstchfuonrgGe(w1a9s7s9)e.rkunde, Koblenz, Z. F. Waser-und
3. OFrorganTiezsattinigonofforCheEmciocnaolmsi,c CSeocotpieornati3o01n aE,ndRDeeavdeyloBpimoednetg,radGaubiidleiltiyn:es Modified OECD Screening Test, May 12, 1981.
4. Dohrman DC-52A Operating Manual, 4th Ed., 1978.
|
001969
. Page 7
=
-
-
-
.
Appendix 1
BEST COPY AVAILABLE
oamuer
ERT pe SMPLE FOI SHEETCORLFHOOIFIED ECOSCACENINGTEST
Exp. 0no: SR#tnR4eEr3 y
areoirea1412,EtSne TrTga--SSP ICREA, =
a Santzalss
Frey wy
c| rn srr |Jo] SOpITEUErM_Ho[cZonE NZaOaytAmmyErcwxn aaoe s| !
f{h oalsemuttieaonnneatrit |Tad--h e =Jos :{1E |peIY ji eje ||
|
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SOLER Seen $C oe THE valves seins toveres inurrision selniLiey
2111 00 values daioreined after membrene Filtration.
:.
AVERAGE of BLanksTM
| RE!
| Apt2
Doc- AFTMERyX 21DAYS{27 | 28
0.7_|0.35 [04a |0:S2| 04s
oy Wa6.92% s
vage 8 Z |
a|
Modified OECDSersoning Teste
cedgygy
=~
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age 9 --
=
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Modified OECD Screening Test:
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SEE Sobium penpeAre PAGE FOR BLANK DATA,
Wekohys
Co
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Eo con Whmeraysrry
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SEE Sodium BENZOATE AGE For BLANK
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TECHNICAL REPORT SUMMARY
XO TH COMANISTNS CENTER = FOE
import gore Btte3 etee TC ue8s
Modified OECD
eening Te on PC=20
=203A nd FC-600
Boo3
a" soeNocsnuemy=p
Lebo
J L
Pn
Oe ona RET SEE Een poriosunmmmaernyt0a CiomdPaibporR-eGqginti mary|96St M CHEMICAL iFrooO rm 2 chemocslroreepared-ns:
To test the repeatability of the modified OECD Screening Test in measuring the biodegradability of Light Water products.
L L
L
%
I.
:
OEEAerGrToRR
i iis
To ovaiake The niagagradansiicy of Antwerp Light Water This study again used the modified OECD Screening Test
products. The products studied in this set of tests were FC-203, FC-203 NFP (equivalent to FC-203A), and
tFhCe-6s0e0.prodAulcstos,tesFtCe-d203wasNFPU,.S.wasFC-a2l0s3o Liontclu30d7e2d. inOnteheof
previous set of tests done in 1980. The results of this
testing were consistent with previous OECD Screening
Test studies on Light Water products. The results
indicate that all of the tested products are readily
biodegradable.
7
-
i
cc: R.L.Bohon-21-pwW-05 D.R.Ricker-23p-1B-10 D.W.Kent-236-pA-04
Hager hy
001979
/-
2
;
MODIFIED OECD SCREENING TEST ON FC-203, FC-203A, AND FC-600
INTRODUCTION
TLhiigshtstWuadtyerwa(sL/Wu)ndeprrotdaukcetns,toPdC-e6m0o0nstarnadtePC-t2h0e3,biousdienggradtahbei)lity of two itenstteirnngatwiaosnalrleyquersetceodgnzbiyed3MMGoedirfmiaendy ObEeCcaDusSecreofeniinngcoTnessits(t!e)n.cy Tihnisthe results of past biodegradation tests done on these products.
MLoadbiofriaetdoryOECshDowSecdreetnhiantg thTreesetsLc/oWndpurcotdeudctsinwe1r9e80hibyghltyhebi34odeEgnrvaidraobnlmee(p?s)a.l FThCe-20p6r,odauncdtsFCt-e3s0t1e7d w(eFrCe-20F6C-2f0r3eAeze(pprreovtieocutseldy).ideGnrteiafiteedr atshanFC-920%03ofNEPt)h, dtihseso1l9v80edtoesrtgianngi.c cSaerebonTab(lDeOC)1 offortahesseumm3arpyrodofucttshesweasrreseumlotvse.d during
Table 1
Results of 1980 Testing on Light Water Products
Product
7
py 3 Degr2adation at7Day: =
rFcC--220036 w,ep}
1233
8950
9043
9931
9932
Hricy-d30r1o7q2uinone
8397
9942
9%97
594
9953
Footnotes:
1 FFCC--220033A.NFP is nonfreeze protected FC-203 and is equivalent to 2 HyCd-r30o1q7uiniosnefrweaeszethperotreecfteerdencFeC-2p0r6o.duct.
Tusheedsea 1s9i80milraersulttesstcmoentthroadd,ictthethTosOeC oDfegrH.adaHteilolnmanTnestappgi).. MHuellllmearn`n)'swho TnOoCrma(ltloytalobtoariganneidcrceasrublotns). tHeisst rfeisnudlitnsg, ofhow8e%veTrO,C arreemovaanlexfcoerptFiCo-n301f7rom acnodntr63adTiOcCt rhiesmovoawln fBoOrD,/FCCO-2D06datian. theHis21-5d-adyaylbonigochTeOmCicDaelgroaxdyagteinondeTmeasntd F(CBO-D3)017resanudltsFCw-2e0r6e, 5r0e8sapnedcti4v0e%lyo.f thTehischeimsicaalsiogxnyigfeincandtemalnedvel(COoDf) Of dowengrapdaapteironclefaorrlya a5r-edayanBOiDnetxepslti.cablTehesdeiscorpeppoasnictye. results within his
HBOeDl,lm/aCnOn'DsmeTaOsCurDeemgernatdsatimaodneTebystthreesu3l4tsEnavrieronamlesnotailncoLnasbiosrtaetnotrywiatndh by ottyhpdi?c"alilnydespheonwdenBOtD,labovarlauteosrietso ornang3e4 AbFeFtFweepnrod6u0c%tsa.nd S90u8chOfrestuheltsCOD. AFForFFepxarmopdluec,tsamacnoulffa8ccttuiroendofby s3u4ch idsatsahowonn i3nU.TSa.blemil2i.tary specification
001980
2
OPEaCgeD S2creening July 3, 1894
Table 2
BOD, Carbonaceous BOD, and COD Data on Mil-Spec AFFF Product
Lab
m5g0/D.RY) c-s(0mgp/.kd? (mcg/okpg) Bo_pcoB c-Bcoopp,2
FC-203 C Lot 501
3m
580,000 600,000 77430,00,00000.79
81
Pace
800,000% 730,000* 783100,,000000
sCaeprscuole
458700,,000000 462800,,000000 793570,,000000 ..660
.l6e0s
Galibnroaciutlhum 41
616,000 671,000 776,4-50 .79
`86
Galibnroaciutlhum #2
670,000 641,000 776,450 .86
82
AS.verD.age
58755,,000000 59904,,000000 78802,,000000 7132
a725
* Calculated BOD's from different dilutions were very inconsistent.
FC-206C Lot 502
3m SPearcceo CaGaplsburlaeith Galibnroaciutlhum #1
inoculum #2
290,000 290,000 338600,,000000 0.78 0.78 325400,,000000 327600,,000000 435700,,000000 00..7685 00..8720 421660,,500000 431201,,000000 441007,,020500 01..6032 01..0718
395,500 374,000 407,250 .97
92
As.verbpa.ge
32753,,000000 33587,,000000 3339,0600,001016 81 8a3
FC-780B Lot 501
m PSaecreco Capsule
240,000 230,000 33320,00,00000.74
7
221500,,000000 222500,,000000 235807,,000000 ..7731
771
240,000 230,000 360,000 167
64
Asv.eroab.ge
23157,,000000 2"3123,,000000 3"292,80,00000.0731 I`0T5
001981
OPEagCeD S3 creening July 9, 1984
METHODS AND MATERIALS 1. Procedure
Tteessttiinngg owfaschpeemrifcoramlesdadionptaecdcorMdaaync1e2, wi1t9h81t.he OECD Guidelines for 2. Chemicals
T(hCe,HsrNeafOe,r)encFe.W.mat=er1i4a4l.s11usaendd wleirneearlaablokryaltboreynzegnraedesulsfoodniautmebe(nLzAoSa).te, Uh.dS.E2anSvirGoonlmuetnitoanlusPerdotweacstioan5.A6g8e%ncyactasivea sroelfuetrieonncesmuaptpelrieidalbyforthe bweiroedegUr.Sa.datFiCo-n203tesLtoitng3.072ThperovLiidgehdt Wiante1r983(Lb/Wy) thteestCommamteericiaalls used CAnhtewmeircpalsinD9i/v8i2sioann,d FFCC--220033 aNnFdP FpCr-o6v0i0dedsabmypleEs.T.pSr.oLv.ideidn b1y980E..T.S.L. A(nntownefrrpeezperovpirdoetdectneod)lotisneuqmubeirvsalewnitthtotheFiCr-20s3aAm.ples, FC-203 NEP PthreiorOECtDo tneusttriinegn,t stohelutLi/oWnanadndrberfoeuregnhcte tmoatteheriaflosllwoewriengdiisnsiotlivaled in mDgO/Cl,conacndentLr/aWtipornosd:uctssoadpipurmoxbiemnaztoealtye 4200 mmgg//ll., LTAhSeapLp/WroxTOiCmatweasly 15 heixgpheerrientcheanhatshatshouwsned thiantprLe/vWioauts40temsgt/inlg is(20nomtg/tlo)xicb.ecauAslesoouhrigher concentrations improve the accuracy of the TOC analysis. Fsoorurctehsisalsltoudwye,d nbyutrtiheentOECsDoluStciroenesninwgereTesptr,epayreeadstuseixntgracbtothandvitamin vsiytnatmhienticsouvricteamianffescotleudtiotnh.e eTxhtiesntwaosf dLo/nWedetograddeatteiromni.ne if the INOCULUM Tphreepairneodculiunmacwcasordaan5c0e/50witmhixtOuErCeD opfrocseodilureesx.tracTtheansdoils,ecwohnidcahrywaesffluent efxrtomracRtaemds,eywaasndaWmaisxhtiunrgetonofCofurensthi,essainndyMinlnoeasmotgaa.rdenThesoislesconodbatrayined ~ esafmfpllueenttaskeanmplferomwasantaheerastuipoenrnabtaasnitn oaft atnheaSctt.ivaPtaeudl MsleutdrgoeWmaisxteedwatleirquor TErielattermenptapePrlanptr.iorThteo ucsoamgbei.ned inoculum was passed through Whatman 54 INSTRUMENT Tlhiemitorgiasniacpprcoaxribomnatealnyaly2zemgr uofsedcarwabsonapeDrorhlmiatner.DC-52A. Its sensitivity
001982
5
OECD Screening JPualgey 49, 1984
SAMPLE HANDLING AND STORAGE
mTahneufsaacmtpulreerpreosfertvhaetioornganmiecthocdarbuosnedawnaaslztyhear.t prescTrhiebemdethboyd thienvolves
adding brings
t1 hedrospampoflescontocenlterssatetdhanHCLpHpe2.r
10Thmel saofmplfeisltweerreed
ssatmoprleed. undTehris
risefrthiagteratthiiosn stionravgiealsmetwhiotdh acalnumipnruemserfvoeil L/lWinecdontcaapisn.ingOusrampelxepserifeonrce
sfreovmeratlhimsonstthusdywwietrheoutdonaeffewcittihnign tohnee DwOeCe.k OfALLitsDOCcomapnlaeltyisoens.on samples
RESULTS AND DISCUSSION
Tsaobdlieum3besnuzmomaatrei,zesthetherefreerseunlctes ocfomptohuinsd,stuwdays..neaTrhliys tcaobmlpeletsehloywsdetghraatded g(r9e5%a)terwitthhiann 780%dayTsO.C rEevmeovraylrweiptlhiicnatetheof 2a8l-ldayof tetshte Lpe/rWiopdr.oducStesvenshoofwed tthhean t9e0n%tTesOtC rfelmaosvkasl.contTaheinianvgeratghee vDaOCrioruesmovLa/lW pfroroduaclltsfosuhroweLd/Wgrperaotdeucrt was greater than 908.
TThheeseresruelstusltsofartehisavetreasgteidn,g aanrde bsheocwanusegraofphitchiasl,lydoinnoFtigusrheosw t1heandact2.ual peaxtatmeprlne gofrapdehgroafda2ticounltuirnesthedegirnaddiivnigduatlhecuslatmuerepsr.oducFti,gurFeC-2303isNFaPn. It srahpoiwds, thbautt otnhcaet dtehegraldagatiporniorof tothitsheproondsuecttofbegdaengratdhaattioint wwaass vvearryiable. Tkhneownreansoronwafsor itthiesxpevcatreida.biliIttymabyetwheaevne tbheeendduupelictaotetheculftaucrtesthwaatsthneot dceuglrtaudriensgretcheeivmeadjordifcfoemrpeonntentlseveofls tohfe vLiiaghbtleWmaitecrrooprrgoadnucitssm.s caTphausb,le tohfe pcuolptuulraetsionprioofrbatcotetrhieaownisteht otfhesreapicdapa(beixlpiotnienetsiahla)d dteogrbaudialtdiounp. in the
Rteevsitienwgofof thtehedaottaherinLT/aWblperod3ucitnsdicaaltseosvatrhiatedrcesounlstisdeorfablryeplaitcadtaeys 7, 1l4e,ngtahndof21.theAglaaign,phatsheisprviaorriattioontheapopnesaerts otfo bbeioddueegrapdraitmiaorni.ly Htoowetvheer, hduoew ltoongthebiosdmeagllradnautmiboenr ooff sianmdpilveisduatlakeLn/,W conoentafirneiqnugentcluyltucoruelsd tonookt tell following the initial lag.
001983
6
OPEaCgeD 5Screening July 3, 1984
Table 3
Biodegradation of LIGHT WATER Products and Refernce Materials
Product/vitanin Source
SSooddiiuumm BBeennzzooaattee//Yveeaasstt--71,(,1) SSooddiiuumm BBeennzzooaattee//VViitt..--12
Mean rFcC--220033/3Y)ea/svte-a2st-1 FFCC--220033//VViitt..--12
Mean UU.SS,. PFCC--220033//YYeeaasstt--12
ean rFCc--220033/(Y5e)a/svte-a2st-1
Mean FFCC--6600003//YYeeaasstt--21
Hean LALSA/S/YYeeaasstt--21
ean
Tm4 DOCnoRmemovalZAftZ er X Days B
9959..86 9848.64 95
21910.57 23900.15 ----(4) 2278..04 9819..08 9925..13 2240.084 8382.066 ----- 9207..27 9850..65 9853..26
92
6246..39 9205..81 ----
9709..72 9965..05 9976..55 97
1123.26 88.55.1 ----
8151..10 9826..54 9867..02 92
67..67 7150..55 ---- 7-6-.(38) 8897..30 98284.28
252.5.8 7a.88 2250..14 3--180) ,8 4290.117 53323.014
Footnotes: ((21)) Uusseedd yOeEaCsDtseyxnttrhaectticasvitvaimtianminsolsouutricoen. as vitamin source. ({34)) F1r7o-mdaAyntswaemrpples198w2e.re only taken for LAS. (3) SFarmompleAntcwoenrtpami1n9a8t0e.d, Orniotginuaseldl.y labelled FC-203 NEP.
001984
READY BIODEGRADABILITY: Modified Screening Test
OECD 301E -- Adopted 12 May 81 Environmental Lab Request No. 9611
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DILLAUS RTEFEERDEE
2 3 7 W % Fs 3
2
DAYS
1.
I"
" OPEaCgDe s5creening July 9, 1984
The modified OECD Screening Test is intended for use on single chemical
substances. L/W products, on the other hand, are aqueous solutions
cgtouhanintdaeil7ni0i%nnegslosaassmiobfxetinuDgrOeC rowefiatdchihilenymib2c6iaolddsea.ygsr,adPauabrrleee.crheegmAailrcsdoae,lds,btyhiwshtihecrheOsuEhlCatDve hagsreattoerbe
reached within 10 days of the DOC removal first exceeding 108. Only
oSneeconodf OtEhCeDrerpelqiuciarteemsentof ofeac7h0% ofdegtrheadaftoiuorn L/wWithpirnodu1c0tsdaytsestofed fmiertst this
reaching 10% degradation. This requirement, however, is not
realistically applicable to L/W or other formulated products
d(imfifxetruriensg).suscTheepticbhielmiitciaelss wtiothbiinodmegirxatduarteisonw.ill Refardeiqluyendtleygrahdaavbele cwhhielmeicatlhecoonmspoentenotfsdoefgrasducahtiopnroodfuctosthemraychsetmairtcaltso diengrtahdeese ipmrmoedduicattselym,ay breeasdeolnaaybelde. forThumsi,xtudriessregofardcihnegmictahliss, setcheondrecsurlittserisah,owwhtihcaht tihse nmoitxture obfiodcehgermiacdaalblec.omponents in each of these products is readily
Cultures tested with the synthetic vitamin solution showed, on average, aas sltiheghtvliytamlionwersoulrecvee.l ofFutDuOCre rOeEmoCvDedscrteheannincgulttuersetss usshionugld yetahsuts eusxetract
yperaesptareextarndactdoessincneot tghievesynitmhpertoivcedvidteagmriandatsioolnu.tion is more difficult to
LAS, which is a readily biodegradable and commonly used surfactant in detergents, did not degrade fully under these test conditions. This
occurred in spite of the fact that its concentration was only 15 mg/l
LAS are shown graphically in Figure 4. Another researcher, has - opfrodDuOcCtsc.omp
ared
The
retaos
otnhe
fo40r
mtgh/el solfowDOrCateuseodf
LfAoSr
tbhieodeLgigrhatdatWiaotenr appears
ctooncbeenttrhaattioLnA'gg'j.nhibTiotecdhemcikcrtohbiisalhydpoetghreasdiast,ionat atthetheendtesotf the 28-day
test period, the remaining LAS containing test cultures were split and
half were diluted to twice their volume with fresh inoculated nutrient
media. At this point, the rate of degradation in the diluted cultures
increased substantially. Seventy percent of the DOC remaining at the
time of dilution was removed within 15 days. Results of this work on
similarly reported a low level of biodegradation of LAS in the modified
OECD screening test. D. Liu found thag Harlon A (dodecylbenzene
sulfonate) had a half life of 39 days.
concLuszons
TTheestsproensenLtighsttudWiateesrrperpoedautcetds tdhoenefibnydintghse o3fM pErnevviiroounmsenOtEaClD SLcarbeoerantionrgy.
Both sets of tests showed that L/W products are "readily
bwaisodgegrreaadtaebrlet.h"an 9I0n% alalftecrase2s8, datyhse. aveLr/aWgeproDdOCuctrsemowvearle mfuocrh L/mWoreproducts
degradable than the very commonly used and "readily biodegradable"
surfactant, LAS.
01989
i
2
. OPEaCgeD S10creening July 9, 1984
REFERENCES 1) oFrogranTiezsattinigonofforCheEmciocnaolmsi,c CSoeocptieornati3o01n Ea,nd RDeeadvyeloBpimoednetg,radGaubiidleiltiyn:es
Modified OECD Screening Test, May 12, 1981. (2) 6g./80a,. 3RMeinTeerc,hniBciaoldegRreapdoarttioNno. of40,"LDIeGpHtT. WA0T5E35R," PPrroodjuecctts99i7n00O1E2C6D00T,est
6/30/80. (3) EDrx.tinH.guiHsehlilnmgannFoaamndCoD.mpoMuunldlser,(Um"wEenlvtigreofnamherndtuanlg Idmupraccth of Fire
WIFeosunterGsieorstmcahni-scthauunmticoetefsl+ H)ydroTS hleogy t (Buwnu daesscanad srtFaIley td fouurt by the gewasserkunde), April 18, 1979. (4) Tp.estmulOlregraniacnaMTa.terTiiatltsizefro,r C"oTmhepleTtOeC DBeigordaedgartaidoantioTnest(D-erATOPCroc-ess to ASbobfauVtoelsTtsta~nteiignenVerbfiaohlroegnisezhuernPrAubfbuanug) "vDoenveOrlgoapnebdischaetnThSeubstanzen `BAubnwdaesssaenrs-tFaolrtscfhourngG(ew1a9s7se9r)k.unde, Koblenz, Z. F. Waser-und 5) bohrman DC-52A Operating Manual, 4th Ed., 1978. (6) MR.arcbpe.lSwDieskhkeerr,, STT uhre.f;ac9 tant 7 Bioode0 ogrrada7 tion, pages 16-17 and 147, (7) Bb.lodLeiut,eriSotruadtyioonn 5B,iodEedgitreaddatbiyonT. ofA. AOnxillienyeanadndS.MarBlaorrnryA,, 1983.
001990
L/W TOC of FC-600
"in Prod Components 2Sultonefoamer 1 Filmer (FC-95) 1Naoctyl sulfate 1 Nadecyl sulfate
rr 0.1 DowicidAe
0.05 Benzotriazol
0.8 Stanley H-277 Starch 12 Buty) Carbitol 81.25 DI Water
Emperical Formula C16 H23F135206 C8F17S03K CBHI704SNa. C10H21 04SNa CI12H170SNe C6 HS N3
assume C6 H10 04 C8H1803 H20
Mw MWoCf
N2 650 192
522 128
232
96
260 120
I 264 144
19
72
146
72
162
96
18
0
TOC 0.0059 0.0025 0.0041 0.0046 0.0005 0.0003
0.0039 0.0711
0
Product ToC= 0.097 g/g
roe
Jo) 9/7
Page 1
001991
