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BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may alsobe referredto as PFOA ammonium salt,Ammonium perfluorooctanoateF,C116, FC-126, FC-1 69,or FC-143. (Octanoicacid, pentadecafluoro-a,mmonium salt,CAS # 3825-26-1) Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-126 and isreferredtoby thetestinglaboratoryas El 282-1 and El 282-2. It'psuritywas notsufficientclhyaracterized, though currentinformationindicatesitisa mixtureof78 -93% test substance and 7 -22% C5, C6, and C7 perfluoroanalogue compounds. METHOD: Method: EPA approved standardmethod numbers 507 and 508A. Type: Aerobic(BOD) and Dichromatereflux(COD) GLP: No Year completed: 1987 Contact time:20 days lnoculum: Not noted RESULTS Chemical Oxygen Demand (COD): 825 mg/kg* Biochemical Oxygen Demand (BOD): 5-Day: 140 mg/kg IO-Day: 500 mg/kg 20-Day: 710 mg/kg *Average oftwo tests. CONCLUSIONS No reliablceonclusionscan be derivedfrom thisstudy. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking4. Summary was createdusingonlya listinogftestvalues.The originasltudyprocedureand raw data were not found. However, therewas a discrepancynoted inthe data values.Data was reportedas "mg/L"by the testinglaboratoryb,ut was enteredas "mg/kg"values inthe3M EnvironmentalLaboratoryarchivesystem withoutproperconversionfrom volume tomass units.Therefore,the data isinvaliads stated. REFERENCES The COD and BOD studieswere conducted by Pace Analyticaolf Minneapolis,MN atthe requestofthe 3M Company, Lab Request number E1282, 5/29/87. OTHER Last changed: 5/24/00 06/02/1987 ENVIRONMENTAL LABORATORY FINAL REPORT LAB REQUEST NO. E1282 REQUESTOR NAME: TE CONTRACT LAB: PACE DEPARTMENT: 053@@ CONTRACT LAB COST: 95 PROJECT NO: 9970012900 3M E-L HOURS: 12 DATE RECEIVED: 02/27/1987 EXP COMP DATE: 05/08/1987 DESC: BASE ECOTOX SET - FC-126 DATE COMPLETED: 05/29/1987 -------------------------------------------------------------------------------- SAMPLE DATE CODE DESCRIPTION RESULT MIN DET LIMIT -------------------------------------------------------------------------------- 1 02/18/87 FC-126 LOT 390 DAPHNIA ACUTE TEST FISH ACUTE TEST *BOD -05 (CARBONACEOUS) *BOD-10 (CARBONACEOUS) *BOD-20 (CARBONACEOUS) *COD (DICHROMATE REFLUX) MICROTOX - EC50 *INORGANIC TESTS REPORT SLUDGE RESPIRATION EC50 COMPLETE COMP0L 14 ,IG-/1 'KIG500 MG/KG 710 G//KKGG 820 GG//KKGG G/KG COMP ATTEE'TTJA C H E D SEE p TACHED COMPLETE REPORT CONTRACT LAB SUBMITTED BY: DATED: REPORT OF LABORATORY iIpd,baomratories,,nc. ANALYSIS May 20, 1987 Mr. Wade Scheil 3M EnvironmentalLaboratory 935 Bush Avenue Bldg. 2-3E St. Paul, MN 55133 Dear Mr. Scheil: Enclosedis the reportof laboratoryanalysesfor samplesreceived 03/02/87. lf you have any questionsconcerningthis report,pleasefeel free to contactTom Halversonor myself. Sincerely, p o r . p inter,Ph.D. Director,LaboratoryServices Enclosures 12-1L 1710 Douglas Drive North 0 Minneapolis, MN 55422 0 Phone (612)544-5543 ace REPORT OF LABORATORY I(ib'oratinoc ries, ANALYSIS 3M Environmental Laboratory 935 Bush Avenue Bldg. 2-3E St. Paul, MN 55133 May 20, 1987 PACE Project Number: 870303508 Attn: Mr. Wade Scheil E1282 Date Sample(s)Collected: By Client Date Sample(s) Received: 03/02/87 PACE Sample Number: Parameter Carbonaceous Biochemical Oxygen Demand(l) Carboneceous Biochemical oxygen Demand(l) Carboneceous Biochemical Oxygen Demand(]) Chemical Oxygen Demand(2) U its MDL mg/L 60 mg/L 60 mg/L 60 mg/L 50 029790 E1282-1 140 500 710 820 029800 E1282-2 830 MDL Method Detection Limit mog/muLg/"L:s ((12)) BCOODD aannaallyyssiisswwaass ppeerrffoorrmmeeddiinn aaccccoorrddaannccee wwiitthh ssttaannddaarrdd mmeetthhooddss ##550087A The data contained in this report was obtained using EPA or other approved methodologies. All analyses were performed by me or under my direct supervision. I4orig@an@i'C Manager 1710 DouglasDriveNorth0 MinneapolisM,N 554220 Phone (612)544-5543