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ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component of FC-783. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotwas notnoted. The testsample isFC783 (cc784-1).Currentinformatioinndicatesitisa mixtureof 1.0% PFOS, 2.0% Sultonefoanier,2.3% sodium octylsulfate1,.6% polyoxyethylene monooctylphenol ether,0.1% sodium laurylsulfate2,5.0% diethyleneglycol monobutyl ether,0.05% tolytriazolaen,d 67.95% water. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the tluorochemical component of the testsample. METHOD Method: Not given. Test type: Staticacute GLP: No Year Completed: 1979 Analyticalmonitoring: Dissolvedoxygen concentrations. Statisticamlethods: Resultswere determinedby graphicinterpolation. Test organism source: Activatedsludgemixed liquorcollectefdromthe MetroWastewater TreatmentPlant,St.Paul,MN Test condition: Dilutionwater: Deionizedwater. Syntheticsewage: Notgiven. Reference and testsolutionpreparation:Not given. Test vessels: Not given. Number of concentrations:one plus4 blankcontrols. Temperature: Not given. Totalsuspended solidsand pH on day oftesting:Not given. Element Basis: Respiratioinnhibitiaosn determinedby oxygen consumption. Remarks: Study terminatedat7 minutes,sincetherewas no indicatioonf toxicitaytthe highestsample concentration. RESULTS Nominal concentrations: Blank controlsand 1000 mg/L Elementvalues: No acuteinhibiteofrfyecotn activatesdludgerespiration rateat 1000 mg/L witha contacttime of7 minutes. Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-783 exhibitedno inhibitoreyffectat1000 mg/L over a 7 minute exposure. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyJ:imischranking3. Study lacksdescriptioannd recordsofthe method followed.The inoculumisnotpropedy characterized.Sample purity was notproperlycharacterizedand thestudy lacksanalyticaclonfirmationof the amount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the3M Company, EnvironmentalLaboratory,St Paul,MN, Lab Request number 4896S, 1979. OTHER Last changed: 6/26/00 'Ae,-4rii@@a)cfIerY OF If -ACTIV.+Tcio 5 4 L N/yeO oea -011 100 JtD R.0 30 7.1 -7 6. T5 30 3.7 3,1 l.Z4 o.5 71.1 1% 0 WIRONMENTAL LABORATT Y SUMMARY SHEET y I t T-T-1 I IF lrc 1 It TT-+ -1, T--T--Tti Iy I zi ar z ui L ui 4 azu 7. ZaW L 2 -:H T. r4. T T T4- 4- -T- Ii f T -TL-L. , -1,r 11 -TA-LX T --F o, t T- i- -t-@+- 0 2 3 4 TIME - minutes