Document gbwwyGnxen0DV8E90kb8qqm8e

AA - 0Z20 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate; may also be referred to as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC206CF. Current information indicates it is a mixture of 0.5% PFOS, 10% diethylene glycol monobutyl ether, 78.08% water, 1.375% acrylic foamer, 1.5% sodium octyl sulfate, 1% sulfonated alkyl ether salts, 6.0% urea, 0.5% triethanolamine, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component o f the test sample. METHOD;_________________________________________________ Method: OECD Guideline 203. Type: Acute static GLP: No Year completed: 1991 Species: Pimephales promelas Supplier: Aquatic Research Organisms, Division of Resource Analysts, Inc., Hampton, NH. Analytical monitoring: Temperature, pH, conductivity, and dissolved oxygen. Exposure period: 96-hours Statistical methods: No calculations performed, based on study results. Test fish age: Juveniles Length and weight: Average length = 34 mm Average weight = 0.23 g (wet) Loading: 0.15 g/L Pretreatment: None Test conditions: Dilution water: Filtered natural well water Dilution water chemistry: Hardness: 80 mg/L as C aC 03 Conductivity: 830 pmhos/cm pH: 7.7 Lighting: Cool-white fluorescent lights. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Once. 001859 Stability of the test chemical solutions: Not noted. Exposure vessels: 20 L glass aquaria (40x20x25 cm) containing 15 L of test solution and an approximate water depth of 18 cm. Number of replicates: 2 Num ber of fish per replicate: 10. Number of concentrations: five plus a blank control Water chemistry during the study: pH range (0-96 hours): 7.1 - 7.7 (control exposure) 7.6 - 7.8 (1000 mg/L exposure) Temperature range (0-96 hours): 21.7 - 22.7 C Conductivity range (0-96 hours): 830 - 850 pmhos/cm (control exposure) 840 - 870 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96 hours): 6.8 - 8.1 mg/L (control exposure) 5.2 - 8.5 mg/L (1000 mg/L exposure) R E S U L T S ________________________________________________________________________ Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L Element values: 96-hour LC50 = >1000 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS ________________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY____________________________________________ _ Reliability: Klimisch ranking = 2. Testing meets the criteria for quality testing. However, the sample purity was not properly characterized and the _ study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ 001860 This test was conducted by EnviroSystems Division, Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-4,1991. OTHER___________________________________________________ Last changed: 6/28/00 001861 i i p - ^- ' i | - v'.; )- '.'?**'.<'! ' -.; i ; ;;y;?`f.* ;. BEST COPY AVAILABLE i. summ . The acute toxicity of FC 206CF to the fathead minnow, Piaepbales pcoaelas, is described in this final report. The test was conducted for 3H Coapany for 96 hours during. June 3 to 7, 1991, at the EnviroSystems Division of Resource Analysts, Inc. in Hanpton, New Hampshire. It was conducted by Jeanne Hagazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Timothy Hard . The test was performed understatic conditions with five concentrations of test substance and a dilution water control at a mean temperature of 22 1 1C. The dilution water was filtered natural well water collected at Hampton; Hew Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of FC 206CF were: 0 mg/L (control), 150 mg/L, *250 mg/L, '400 mg/L, 600 mg/L, and 1,000 mg/L. Nominal concentrations were used for all calculations. i' Fish used in the test were purchased from a commercial supplier (Aquatic Research Organisms, Hampton,' Hew Hampshire) and acclimated under test conditions for more -than 15 days. After 96 hours of exposure the control fish had an average.total length of 34 mm and an average wet weight (blotted dry) of 0.23 g;>resulting in a loading rate ..of approximately 0.15 g/L. All fish, were in good condition at the -beginning f the study. ; A- ' . .^..Exposure;of fathead minnows to^the test substance resulted in a 96 ;)^fhour^.;LC50 ^.greater than 1,000 mg/L FC 206CF. . Ho sublethal effects were y &observed in any test vessel dnring'the 96 hour exposure. ' 3- mm Vr V * #? X5; t p-. 'v- V --e ^ `I; .-'v-. - fcr,'vi` S',5? i * 001863  '1 - s* .V ^--V '' '*- ^ ' ^ 1 -V- ,. - BEST COPY AVAILABLE % in . . iron or tables Table 1. Chemical characterization of a representative sample of natural veil water used as dilution water for toxicity test;!,V,A.^- . Table 2. Survival data from toxicity test Table 3. Median lethal concentrations (LC50s) from ' toxicity test ', Table A.l. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity - test -td ^ f$&-r<>*** t* L ;. d, *-,>`S-'\l ', 3 rH 10 13 5= ife ; . s * ymsr T O -*; *;a .. *'j.' a**".. .r . ,T E&tJy' 1 'S 'I i l k - -st I Mil 001865 IV 'IR P I i!, 1,?'' ' v"*"1" ?*''. * < i*? ' ' '. ' ';>/'. . ; ^ES7 COPY AVAILABLE V:V, SJ5? i t . irraorojcTio* This study was sponsored by 3M Coapany, St. Paul, Minnesota. The objective of the study was to determine the acute toxicity of FC 206CF to the fathead minnow, a freshwater fish. The report contains sections that describe the aethods and aaterials eaployed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. T. METHODS AMD MATEEIALS TEST SUBSTANCE: FC 206CF (EnviroSysteas Sample Muaber 2938E) was delivered to KnviroSysteas on May 23, 1991. It was contained in a 4 oz plastic bottle that was labelled with the following intonation: "J1884-4, FC206CF". FC 206CF (a light brown liquid) was supplied by 3M Coapany, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test aaterial was stored in the dark at rooa temperature. A reserve saaple (approximately 1.0 graas) will be retained at EnviroSysteas for a minimum of 10 years. DILUTION WATER: ' Mater used for accliaation of test'organises and for all toxicity testing was '*collected from wells at EnviroSysteas in Eaapton, New Hampshire. Water was stored in 500-gallon polyethylene tanks, where it was ^aerated. Results of chemical analysis of a representative saaple of wate.r'i'9ca--r'e(presented--\i-1nTabVl'Oe t. -,,'1'.~,.v7.- TEST "OR"GxAN-ISMi::. Svenile ; fathead"|ainnows^ipYed^M';?test organisms were from a ):j single'lsource'. andijwere^identifi^jfusing^an appropriate taxonoaic key fA.. T h e y ^ l w e r ^ p ^ c h M e ^ f r o ; ^ ^ i e r c i a l ^ n p p l i e r (the";Aquatic .Research O rgaim ;^l)ivifioi^ i^M M ^i|fi^yatif'j| ^^B aapton'^^^B aapsliii^ii|and ^'"^accliaatedjratlithe^En^foSysteM^facilityif^^^ s8$. y fis^wer&: weighs .".?^_e_i_st_p_g_^(_i_s_h_L^_w_e|_j j s m j8 days.^Control treated&for"diseas^and}.they^wnrdfrefeoflap_p_aa_rr_eenn_tt sickness,?Injuries^ and abnormalitiesatIth^^ginining^^i!%th<^te8t!^ipufinartheYacciimation*period - mi 001866 Ir*< .-j i,,, ,. V J .&GL 4 V - 'S* 2 'HSf'S. -hV* fi '.if''" " . r- BEST COPY AVAILABLE Tablai. Chemical characterization of a representative sample of s..v >:r;- ,v natural well water used as dilution water for toxicity test Parameter Unit of Measurement Reporting Limit Measured Value pH '*-.v Conductivity > pH units umhos/cs V~` . 7.7 830 Hardness mg/L as CaCOi 80 Organochlorine pesticides Organophosphorus pesticides i . : >';*'V ?.'"* Polychlorinated biphenyls a . -> ug/L ug/L : ' ug/L -. ' 0.5 y ;S / "V - **'.- S-v'' : -0.5 ^4 1 4 - J" ` - NO HD HD i:?g1V HD;^not^detectdlat^r abovethVfreportinfllimit. dilution V*. -ei'v' I **. i W & fr ' ^1 001867 m **?}$?* v$. sK; MK BEST COPY AVAILABLE TOXICITT TESTING: X screening test with the test substance was not conducted and historic data was used to choose the test concentrations. The definitive toxicity test was performed during June 3 to 7, 1991. It is based on procedures of the OECD (1984). The test was conducted at a target teaperature of 22 i 1C with five concentrations of test substance and a dilution water control. Eo stock solution was prepared and test siaterial was added directly to dilution water contained in the test vessels without the use of a solvent. Nominal concentrations of the test material were: 0 mg/L (control), 150 mg/L, 250 ag/L, 400 ag/L, 600 ag/L, and 1,000 ag/L. Twenty fish were randoaly and equally distributed among two replicates of each treataent. The.test was perforaed in 20 liter glass aquaria (approximately 20 ca in width, 40 ca in length, and 25 ca in height) that contained 15 liters of test solution (water depth was approximately 18 ca). Test vessels were randomly arranged in an incubator during the 96 hour test (a random numbers table was used to select the location of each vessel). X 16 hour light and 8 hour dark photoperiod >'was automatically maintained with cool-white fluorescent lights. Xeration was not employed to aaintain dissolved oxygen concentrations above acceptable levels. Fish were not fed during the test. v-` The number Y of . surviving organisms and the occurrence of ^ subletbal effects ;(loss:; of 4 equilibrium, erratic swimming, loss of ;reflex, excitability,^discoloration, or change in behavior) were 4 determined visually.and recorded initially and after 24, 48, 72, and 96 hours. Dissolved oxygenlYSIHodel 57 meter; instrument number PRL-3), pB (Beckman' model pHIfl2^eter;;finstrument number PRL-51), conductivity (Labcoap. SCT meter4^instrumenti|number; .PRL-3), and temperature (XSTM ;mercury . thermometer;^thermometer$: number 41686) weremeasured and ^recorded daily in eachltest chamber* that contained live animals. i * STITISTICXL METHODS; r '^.Results^of^ ........ - - *toxicityjvtesfc*?;could, not.4be ^interpreted ?.by .. X standard ^statistical ^echniquei^TStephan^ 1983).because-greater than $.50% survivaltbccurredS lallfAeRjieiJconcentrationsii^il^J^^'-.'^^.'^*.-'^ ^ .......' >4 m ' 1 >T' Jm *r 001S 68 All teat vessel* containing FC 206CF sere initially cleat and regained clear throughout the test. Biological and water quality data generated by the. acute toxicity test are presented in Table 2 and Appendix A/ respectively. One hundred percent survival occurred in the control exposure:^ Control fish had an average total length of 34 as, and an averageTwet weight (blotted dry) of 0.23 g at the end of the test. > Loading rate during the toxicity test was approximately 0.15 g/L. '-:z-' -... H M m &r*Ai i ,-r &m] A V,! i* . 1-/J",,*'-W~ -s'*>''.>,-'v`~* & 3 ^ 7 * 'v/&. ^1*5 ' :i S-V. H-r/i' v,'rh ^ ^ 4'`V V vos, ' 1': y*->'r ,r 4 . 1 >K ' r,' ,V'l ' .Jr*;,: >*' I V 5* ,, w & T iMt 3* #(li*a lethal:concentration* (LC50s)froa toxicity teat s?J-'i Exposure period LC50 n S f3' . - ?'-*' '^24, hours s >1,000 mg/L 95 percent confidence liait LC50 calculation method - -X x -fj , H^xv'r ' ltjis, ; 000 ,ag/Ii V72 hours; >1,000 ag/L i ' i S x , * >1,000 ag/L O V. BEST COPY AVAII ,- -i fi ,1 ito - `l^ ,>i ik-vi s&* ' ,/SCN v. <<?k>fi,'>1'>"- k, r^`ifcr* vi- S 'X ifiJD TD ^ fX 001871 001872 001S73 G'WfS' *'<' -v:&'b ' -I a '. V : "' ' F '" ' '' ; , * m .% v '. ' ' P ::'v " ' -'-i \i m *' i| , SJ\lBEST COPY AVAILABLE Ttbl A.l. Conductivity, pH, tenperature, and dissolved oxygen .concentration aeasured during toxicity test *: i >V 0V' " ' ,;V ~ p ' V'f. M A m A * -' Noainal concentration (ng/L) Rep.. Conductivity (unho/cn) PB 0 (con) 150 250 400 600 1,000 1 830 830 840 840 850 2 830 840 840 850 850 1 840 840 840 850 860 2 840 840 840 850 850 1 830 840 `840 850 860 2 840 840.840 850 860 1 840 830 840 850 860 2 840^ 840 850 850 860 1 840 840 850 2 840 840 850 1 . 840 850 .850 2 , . 840 1.850 850 850 850 .850 860 860 860 860 870 Bote: r(con) control; p `Ifg i m 7.7 7.3 7.1 7.1 7.4 7.7 7.4 7.1 7.1 7.4 7.7 7.7 7.6 7.4 7.5 7.7 7.7 7.6 7.4 7.5 7.7 7.7 7.6 7.4 7.5 7.7 7.7 7.7 7.4 7.6 7.7 7.7 7.7 7.5 7.5 7.7 7.7 7.7 7.5 7.5 7.7 7.7 7.7 7.5 7.5 7.7 7.7 7.7 7.5 7.5 7.7 7.7 7.7 7.6 7.6 7.7 7.7_, 7.8 7.7 7.6 *&s> * . , Jv iiSJt'i/jJ &&>* ,tr I s.i - & 00187 I ? ? ' ; . - A-S,- j ..,. `^.j- i- yMii'f I7i Tabi* A.l. Continued. Mouinil concentration (ag/u Rep. Tenperature (C) 0 24 48 72 96 hr hr hr hr hr Dissolved Oxygen (ag/L) 0 24 48 72 96 hr hr hr hr hr 0 (con) 1 22. 2 22.2 22.1 22.2 22.4 2 21. 8 22.0 22.0 22.1 22.4 150 1 22.0 22.*2 22.3 22.0 22.7 2 22.0 22.0 22.0 22.1 22.4 250 1 22.0 22.0 21.9 22.0 22.3 2 21. 9 22.0 21.8 22.0 22.3 400 1 21. 9 21.8 21.8 22.0 22.3 2 21. 7. 21.7 21.8 22.0 22.3. 600 1,000 -V.-tv i-v. - -**< 1 21.8 21.9 21.8 22.0 22.3 2 , 21. 8 21.8 21.8 22.0 22.3 ( ,, ' V 22.0 21.8 21.8 22.0 22.3 22.0 22.0 21.8 22.1 22.4 X?i4 : .Mote:'-'(con) control; 8.1 8.1 7.2 7.0 6.8 8.1 8.0 7.3 7.2 6 .8 8.0 8.0 6.5 6.4 6.2 8.0 7-9 6.5 6.4 6 .3 8.4 8.0 6.5 6.5 6 .3 8.4 8.0 6.5 6.5 6 .4 8.4 8.0 6.5 6.5 5.2 8.4 7.9 6.5 6.4 5.5 8.4 8.0 6.7 6.6 5.2 8.5 8.0 6.7 6.6 5.7 ?-* 1 ,^ 5*/'' -<rV*.* >`J R W V *>V? ilf ;.O js 1 ,T'j-.f! 001S7 * v *'