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MICROBIOS MICROTOX TOXICITY TEST
TE$T_SUB.STANCE_______________________________________ _
Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-3003. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not given. The test sample is FC-3003. Current information indicates it is a mixture of 1% PFOS, 15% diethylene glycol butyl ether, 79.39% water, 2% Sultone foamer, 1.5% sodium octyl sulfate, 1% sodium decyl sulfate, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample.
METHOD:____________________________________________________
Method: Microbios Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1997 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbios' Reagent. Test conditions:
Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A 1 g/L primary stock solution was prepared b^ dissolving 100 mg of test substance in 100 mL of Millipore Milli-Q water. The pH of the stock solution was then adjusted from 5.7 to 7.7 using 1.0 N NaOH. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss
RESULTS____________________________________________________
002693
Nominal concentrations: Blank control, 62.5,125, 250, and 500 mg/L
Element value:
5-minute EC5o = 311 (289 - 335) mg/L 15-minute ECso = 171 (161 - 181) mg/L 30-minute EC5o= 120 (118 - 122) mg/L
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
CONCLUSIONS_________________________ ___ ______________
The test substance 30-minute EC50 for Photobacterium phosphoreum was determined to be 120 mg/L with a 95% confidence interval of 118 to 122 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133
DATA QUALITY________________________________________________
Reliability: Klimisch ranking = 2. The study meets all the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES________________________________________________
This study was conducted by the 3M Environmental Laboratory, St. Paul, Minnesota, Lab Request number R1331, 1997.
OTHER______________________________________________________
Last changed: 6/26/00.
002694
ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET 9 3 l crDLatRe:: 3R- 1\35-59\1 Analyst: "Tu^s
TEST SUBSTANCE: F c - 3 0 0 3 ( \ r r r C
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1. Preparation ofStock Solution
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* Final Concentration o f Stock Solution: \ ^ \ L.
2. pH Adjustment of Stock Solution
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3. Osmotic Adjustment of Stock Solution
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4. Appearance ofpH and Osmotically Adjusted Stock Solution
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* Color: eJecirs
* Comments:
5. Comments
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FILE: R1331-2.K5 S econd Run FC-3003
MICROTOX DATA REPORT Basic Test
AFFF
(A u s tra lia )
3-13 -97
TKS
T e s t T im e : NUMBER
5 m in u te s
JO /IT
CONC.
O s m o tic A d ju s tm e n t : Na C i
CR/GAMMA
<7% E F F E C T AV % D I F F .
i11 1o 1o
i
C o n t r e .! 9 6 . 3 9 / 8 4 . 0 8
0.8723 li
C o n t r o l 9 8 . 5 6 / 8 3 . 2.1 0 . 0 0 . 8 4 4 3 tt
....
1 93. 56/ 70.36
62.5000
0.141 #
12.4
2
92. 15/ 70.94
62.5000
0.115 U
10.3 11.3 Q 1.0
3
99. 36/ 64.57
125.0000
0.321
24.3
4
98. 54/ 63.70
125.0000
0.328 #
3 4 . 7 2 4 . 5 <? 0 . 2
5
94. 67/ 48.38
250.0000
0.679 li
40.5
6
9 4 . 86/ 47.57
250.0000
0 . 7 1 1 1
41.6
41 . 0 <7 U . 6
7 102. 12/ 29.35 500.0000 1.986 # 66.5
8
9 9 . 19/ 30.22
500.0000
1 . 8 1 7 11
64.5
65.5 O 1.0
CR - CoCORRECTION FACTOR - 0.8583
# Used fo r c a lc u la tio n s
EG50 311.0 m g /L ( 95% CO NFIDENCE R A N G E :289.0 TO 334.7 )
S ig n a tu re CONTINUED TO NEXT P A G E . . .
TEST DATE: 3 - /3TIME:______ _
002696
FILE:R1331-2.K5
PAGE 2
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EC50
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ESTIMATING EQUATION: LOG C 0.7769 x LOG b +2.4928 95% CONFIDENCE FACTOR: 1.07603 FOR EC50 COEFFICIENT OF DETERMINATION: R "2 = 0.99416
002697
P3 H o <r T,
FILE: R1331-2.K15 Second Run FC-3003
MICPOTOX DATA REPORT Basic Test
AFFF (Australia) 3-13 -97
TKS
Test Time: 15 minutes
Osmotic Adjustment ::NaCl
NUMBER
1 0 / IT
CONC.
OR/GAMMA % EFFECT AV % o DI FF
Control 96.39/ 86.73 0.0 0.8998 tf Control 98.56/ 87.78 0.0 0.8906 #
1 93.56/ 64.20 Uot 92.15/ 65.29 3 99.36/ 53.26 4 98.54/ 51.49 5 94.67/ 32.83 6 94.86/ 33.21 7 102.12/ 15.73 8 99.19/ 15.38
62.5000 62.5000 125.0000 125.0000 250.0000 250.0000 500.0000 500.0000
0.305 0.263 0.670 ft 0.713 ft 1.581 ft .1.557 ft 4.812 4.773
28.3 20.9 40.1 41.6 61.3 60.9 82.8 82.7
22.1 <7 1.2 40.9 <7 0.8 61.1 Q 0.2 82.7 <7 0.1
CR - CoCORRECTION FACTOR - 0.8952 fi Used for calculations
EC50 170.8 rag/L (95% CONFIDENCE RANGE:161.0 TO 181.2)
Signature
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CONTINUED TO NEXT PAGE:..
TEST DATE: 3 - 1 3 ^ 1
TIME:_____ ,,___
002698
FILE:B1331-2.K15
PAGE `2
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10 ZDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD? 3SL0P = 1.1826
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EC50
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ir DD 100
DD DDDDDDDDDDD CONCENTRATION
DDD'DD 1000
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DDDY J0E4
ESTIMATING EQUATION: LOG C = 0.8430 x LOG b +2.2325 95% CONFIDENCE FACTOR: 1.06065 FOR EC50 COEFFICIENT OF DETERMINATION: P'2 - 0.99691
002699
Qg (p c \ "I
FILE: R1331-2.K30 Second Run FC-3003
MJCROTOX DATA REPORT Basic Test
AFFF (Australia) 3-13 -97
TKS
"V < S 8<^*o x
Test Time: 30 minutes
Osmotic Adjustment :NaCl
NUMBER
10/IT
CONC.
CR/GAMMA % EFFECT AV % '1 DIF
Control 96.39/ 93.39 Contx'oJ. 98.66/ 93.36
1 93.56/ 62.39 2 92.15/ 62.84 3 99.36/ 46.35 4 98.54/ 45.93 5 94.67/ 26.50 6 94.86/ 26.61 7 102.12/ 11.29 8 99.19/ 10.50
0.0 0.0
62.5000 62.5000 125.0000 125.0000 250.0000 250.0000 500.0000 500.0000
0.9689 # 0.9471 it
0.437 0.405 # 1 .054_ it 1.055 # 2.422 2.415 7.665 8.050
30.4 28.8 51.3 51.3 70.8 70.7 88.5 89.0
%-
29.6 <3 0.8 51.3 <? 0.0 70.7 <3 0 . 0 88.7 Q 0.2
CR = CoCORRECTION FACTOR = 0.9580 # Used for calculations
EC50 120.3 mg/L <35% CONFIDENCE: RANGE: 118.2 TO 1 4
Signature CONTINUED TO NEXT PAGE!
TEST DATE: J r H s 3 l .
TIME:____ ____
002700
17
R\
3 - I3-<V7
FILE:R1331-2.K30
PAGE 2
10 ZDDDDDDDDDDDDDDDDDDDDDDDBDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD?
.9SLOPE = 1.3811
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10
100 CONCENTRATION 1000
l(iE4
ESTIMATING EQUATION: LOG C = 0.7240 x LOG b +2.0802 95% CONFIDENCE FACTOR: 1.01758 FOR EC50 COEFFICIENT OF DETERMINATION: R 2 - 0.99999
002701
