Document gb6BzqQ0mypb0XVqGLbE8wj5V

fiKSLS.6 - O Z 2 9 ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE____________________________________________ Identity: A mixture containing periluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonicacid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-600 (SCAS Treated Effluent*). Current information indicates FC-600 is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentration o f impurities that was treated in a SCAS reactor. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. This study is the second of 3 daphnia studies conducted to determine the toxicity of FC-600 after being treated through a SCAS reactor. This study used the supernatant of a mixed microbial culture that initially contained FC-600 at 667 mg/L*. The effluent was collected after 4 hours of treatment in a SCAS reactor. The inoculum used came from the Metro Wastewater Treatment Plant, St. Paul, MN. * The study references a "1,000 mg/L" concentration. This value is not accurate as it does not include dilution by the sewage mixed liquor. METHOD:____________________________________________________ Method: Not noted. Type: Static acute GLP: No Year com pleted: 1977 Species: Daphnia magna Supplier: Not noted. Analytical m onitoring: Dissolved oxygen Exposure period: 48-hours Test organism age: Not noted. S tatistical method: EC50 calculated by graphing data and by probit analysis. Test conditions: D ilution water: Carbon-filtered well water, aerated. D ilution water chem istry: Dissolved oxygen: 9.4 mg/L 0020OS Lighting: Not noted. Stock and test solution preparation: Volume/volume addition Exposure vessels: Not noted. Number of replicates: two Number of daphnids per replicate: 10 Number of concentrations: six (control was written up as a separate study) Water chemistry during the study: Dissolved oxygen (final): 5.6 mg/L (control) 0.6 mg/L (100% effluent exposure) RESULTS___________ ________________________________________ Nominal concentrations: 5.6%, 10%, 18%, 32%, 56%, and 100% Element values: 48-hour EC50 = 12.6 (10 - 15) % Element values based on nominal concentrations Remarks: Testing was conducted on the SCAS treated mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The SCAS treated FC-600 effluent 48-hour EC50 was determined to be 12.6% with a 95% confidence interval of 10 to 15%. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking * 3. This study lacks description of the methodology used, reporting of test conditions and information on the amount of test substance remaining after SCAS treatment. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, 1977. OTHER______________________________________________________ Last changed: 6/16/00 002009 740101 f ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET f Type Test H & 9 6 Hours ~TQieeA)CS STUpy OAPMfMA Test Organism Fathead Minnow__________ Material Tested f C (eOO (t r Ia t e p ) * * 1.000 "Avg". Avg. Weight _________ Size Date Started Time Started g /8 /? 7 SLIU11Date Completed_____ Plotted LCcn i Z ^ o Dilution Water Carbon Filtered - Well Water TP <f.if ) 002010 best copy available 002011