Document gb2MN4XXLVggYze2r2X7YN4Jq

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE ldent4: A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-603F. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 501. The testsample isFC603F. Currentinformationindicatesitisa mixtureof0.5% PFOS, 7% diethyleneglycolbutylether,82.95% water,2% acrylicfoamer,4% Alkylether sulfate1,.45% Xanthan gum, 1.4% calciumacetate,0.07% N-(3Chloroallyl)hexaminiucmhloride0,.5% tdethanolamine,0.05% sodium hydroxide,0.03% cis-1-(3-Chloroallyi)-3,5,7-td-aazzao-n1iaadamantane chloridea,nd 0.05% tolytriazole. The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately retlectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline202. Type: Acute static GLP: No Year completed: 1994 Species: Daphnia magna Supplier:In-houseculturesO.riginalloybtainedfrom U.S.EPA-NETAC, Duluth,MN. Analyticalmonitoring: Temperature, pH, and dissolvedoxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticamlethods: Not applicable. Test conditions: Dilutionwater: Carbon-filterewdellwater (3M Well #2, St.Paul,MN). Dilutionwater chemistry: Not given. Lighting: Not given. Stock and test solution preparation: A primarystock solutionof2000 rng/L was prepared,dilutedto200 mL and dividedinto4 -50 mL replicates. Aliquotsofthe primarystocksolutionwere used topreparethe remaining concentrations. Exposure vessels: 100 mL vesselscontaining50 mL exposure solution. Number of replicates:4 Number of daphnids per replicate:5 Number of concentrations: fiveplusa blankcontrol Water chemistry during the study: pH range(0-48hours): 8.4- 8.4(contreoxlposure) 8.4- 8.4(1000 mg/L exposure) Temperature range (0-48hours): 20 - 22 OC Dissolved oxygen range (0-48hours): 8.3- 8.3mg/L (controelxposure) 6.4- 8.3mg/L (1000 mg/L exposure) RESULTS Nominal concentrations:Blankcontrol1,00, 180,320, 560, and 1000 mg/L. Elementvalues: 48-hourECso=>1000rng/L Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-603F 48-hour EC50 was determined tobe >1000 mg/L, the highest concentrationtested.No mortalitoyr sublethaleffectswere observed. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.This studymeets the criterifaorquality testing.However, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmabonofthe amount offluorochemicalproportioninthe solution. REFERENCES This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Ml 655, 1994. OTHER Last changed: 6/26/00. 3M ENVIRONMENTAL LABORATORY Daphnis nogna 48-hour Toxicity Test Data Summary LR: Date: Analyst: MISS5 5/27/94 J3D SAMPLE: FC-603F (Lot#501) Sample Conc. (mg/L) 100 180 320 560 1000 No. Organisms Exposed 20 20 20 20 20 % Immobilized0 24 hours 48 hours 0 0 0 0 0 0 0 0 0 0 24 h EC50 (95% C.I.)= 48 h ECSO (95% C.I.)= > 1000 mg/L > 1000 mgtl ECSO Estimation Method: N/A ENVIRONMENTAL LABORATORY LR nl I 48-HR ACUTE TO)OCrrYTO DAPH14A. DAPHAIIAMAGNA TYPE TEST: - STATIC STATIC RENEWAL TEST SUBSTANCE- ip(,- (cc ;@@ ( I-c@-\ * ,sc, \"I@ DILUTION WATER- CARBON-RLTERED WELL WATER (3M WELL #Z ST. PAUL. Mtn TEMP. a4 *C. pH D.O. P.7img(L TEST DAPHNID SOURCE: U.S.EPA-NETAC. Duluth,Mn DATE RECEIVED. DAPHNID AGE: c24 HR OLD NEONATES NO. OF DAPHNIDSrrEST CHAMBER: 5 LOADINGRATE: ,-c-%%,,,TESTSOLUTION VOLUMF- 50 ML SIZE OF TEST VESSELS: DATEINMATED: DATE TERMINATEI)-. -,K- 4 -1- c\,-k TEST VESSELI SKI BK2 BK3 50 lldTIAL TOTAL CONC. AMOUNT mgfl ADDE[R CONTROL CONTROL CONTROL CONTROL MORTALITY 24-HR 4844R PH I c1 D.O. mgn- '6 , '--@ is, 100 ML %C, C-. 1.2 L 1.3 ("1 1.4 -A 'A % zi 3A.L c 2-2 Z3 Z4 3.1 1;"-C 3.2 r 3.3 C' 3.4 C. 4.1 C 42 c- 4.3 CC ) C-. 4.4 C. 5.1 6.2 5.3 5.4 C, fl,Li COMMENTS ITEMP.*C IDATE @ ANALYST ECW (96% r-1.)mWL Lc%Nk.@) e"4 '-@CC-C, (-,C;C. ANALYST: