Document ganEr2mRpwJa6y7eV9LynG5aa

AR 826 - O762 MICROBICS MICROTOX TOXICITY TEST IEST ~S ~U ~ BSTANC 00E 00 Identity: Mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC203CF. Current information indicates it is a mixture of 1% PFOS, 20% diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3% sodium octyl sulfate, 1% triethanolamine, 2% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data `may not accurately reflect toxicity of the fluorochemical component of the test.`sample. M0 ETHOD: 0000 Method: Microbics Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1991 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: ECS50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A primary 2000 mg/L stock solution was prepared by dissolving 200 mg of test substance in 100 mL of Millipore Milli-QTM water. The pH of the stock solution was 6.6. An osmotic adjustment was made using 200 mg NaCl dissolved in 10 mL stock solution. Appearanceof test solutions was noted as "clear, colorless". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Numberofreplicates: two -- Number of concentrations: Four plus a blank control Element basis: Percent light loss R0 ESOT0 S 0000000 Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L 001251 Element value: ~~ 5-minute 15-minute ECso ECso = = 470 360 (420 (330 -- - 520) 390) mg/L mg/L 30-minute ECeo = 280 (260 -- 300) mg/L Element values based on nominal concentrations. Remarks: Substance RTeesmtairnkgswafiseldc.onTdhuectveadluoens the mixture as reported apply described in the Test to that mixture and not the fluorochemical proportion alone. c0 onclus0 ions 000000 d`Tehteertemsitnesdubtsotbaenc2e8300-mmgi/nLutwietEhCaso95fo%r Pchoonffiodbeanccteeriintuemrvpahloosfp2h6o0rteoum30w0as. mglL. SPuaublm,itMtNe,r:5531M33Company, Environmental Laboratory, P.O. Box 33331, St. bataqQuayry 000 HReolwieavbielri,tyt:heKisiammipslceh rpaurniktiyngwa=s2.notTepsrtoipnegrlmyecehtasraalcltecrriitzeerdiaafnordqtuhaelisttyutdeysting. tacks analytical solution. confirmationofthe amount of fluorochemical proportion in the R0 EFEREN0 CES 00000 This study was conducted by the 3M Environmental Laboratory, St. Paul, MN, Lab Request number J1884-3, 1991. ow0 er 00000 Last changed: 6/28/00. - 001252 as i N i j rll -- ; 1 -- IE hows iy a_" or e-- ee i = mr I 2 Pi 30s Ea LAE fais 0 EWRONBITAL LSORATORY ge RR ---- Ree ene Ye 4 8 3 = Ei vo mis| eB LE= a 2 = = = ee TT Be a] ee = R f r T n LTe e ee Ener 5 IRL SL Ty BEE ey TE ECB THES rE e ya A : rT SESE op TRY| MN Ly wi2 sGts un BRo aAoRaEl n aaApEoe Be iorRERE 4TEST SUBSTANCE: Q-203CF Loi JO, BEST COPY AVAILABLE 2 Piesr owe: _7/1a/ai 8% B :a: -- h fliapr ee aarvmeantree Sr poaae | | Ta | |1g 0S0EE5I7E | 0y0o || 3332 i z TEL LEIA Calman Li | Bs il7 30. | 23 Dpliaies| CARIBE| 29.0| 23 casa lamm E 148oeg e oan ok Ee pb i BE ae Ah HE ofSa ih 2 rE jf - 4 IEEE | Source! 14 aR oR 3 eenpa EN TE SN) } fea Ci de vsEn amb nmr ote Bt laden LE I es SE ors fe ha " , : E R PREORTa rE TEORE pale singe BAT BRise1 rR i x mep m aieER E ELEE Er gsr Loni sin wan. assets rig 46" wa Ne To a 2 gT oo agmmae oswseoB.l dmad UiGaalaly RBEoeeiseee aimieeys 3sR8Rortte2i1are TSiorl B BeteanE t R Noruon n425h0s SsEm T2B ovtseSoesal0m3r3a3 =5 am ea - RA {bia ipoaspenoZh99e 1 57 : i 1) fda yr, ~- fTis n Mis.a2z (41747210 519.255 i O+6tmy (54 Esp= 470 (4a0-5m2g0)> BEST COPY AVAIL ._ i R'=0.99110 SL=O0.P90E71 INTERCEP=T +6.1429 2 ~ Bg: lio ies 8 se cE SCeipra EeET G SoE r E del R REE eC d ea 0 Be A I LC a TR aah he aad aaa La Bel LL 7 Le i RE PREi Sed i L SOL saa]e SRE e a a e abd cv,I NLA a EEEOE as f5EhltR a F E e irR a ens eRBN ESREeisASRER SLeEOR R SL TI ) EA Ge iE oF A in Tees CEs Gerth Lied H " k Ri | ' RBT EST CE OPYAT VAILABLE e Tames razed ; as ne mcharoxtr) oath SEEIEST COY rCaoscr Lot 101 15min wep. 31084-3 7/19/91 sub _ ULE PAIR `cone. torte 085 eer 2 0 7 IB TP3 TTOomEm1s2m 5e.:300 9wRa3eo7w0nn$7nooe.0 T83855E33 oTaFieywnr o3i2am 5 ui 10833 Ldemeaone wEolsEoiSrsed ii$a)30 wmPeEe e 88 n io 5%1000.000 8.07 100 20 sham 95 ? } 8 stank <Ba/Rn 99 / 40 0 we BLANK ec so RAi 'TSIiOR=R0s.e40|40 ( S3a.086 TO 389.097 6-cbs TM (ivGegy'TM ECs=, 3L0(330 -- oymae R 0.99329 SL=O0.P87E 92 INTERCEP=T 5.8887 BE SE a nd sf eed : - ks i i ega : S F ee lJ ] gFualsTioRioaczit SatE5sieo> FETet = <Cse B (RRR ETsa ile iiaE pe a E5a5 f a e GocnaelEER in0 {| Se dodenma 0 NCTE } 3 Si CE LARREIERS aa oo 5 Fray " SE eR oF ahr - Scr ee fl HoBR RR Bn 4 Bc 8 a ' NE P iTwe TRTR ERE e Sk : 77 "BEST COPY A\ LABLE Lr %4 of Gn HIGRETOkcr) Daa ET 5 fe-zoieriior 101 So-ain exe. 1884-3 7/19/91 sab nh Cee. 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