Document gMrRDJDNNR3Nv8E9jnp2wMme
Wildlife International, Lid. ae
ProjectNumber $54A-108
pros:
AN EARLY LIFE-STAGE TOXICITY TEST "WITH THE FATHEAD MINNOW (Pimephales promelas)
ADDENDUM
`WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454A-108 3M LABREQUEST NO. U2723
SerieUs.S8.50EOn.vPiEPreToonSlmoeNgneutama-llbEePfrrfoe0tce.tcs1tiT4oen0stA0gGueindceylines
(OECD Guideline 210
at eveon 7710ad heprywasdetermined 0 be 6 9%.TheSpr "This study was reported using a test substance purityof 90.49%. The test substance
requestedthatthe rebseru evil sedt bassed`onthemostrecentpurityanalyses. Theresults Npora evitoustlyepwraemssemp natedtdewetorreecsailmcpullyatceoarnraelcytteidcbalystthaenrdaatridocoofntcheenttrwatoipounrsiotiresmoerasaufraecdtcooroncfe0n.9t6r0a3ti.ons
fromorigriawndaatla.Assuch,minorerrorsduetorounding maybepresent. Attacihstehde
revised summary page from the final report and the most recent. certificate ofanalysis.
Z 37
=s 2382 = 53
Wildlife International, Ltd. 2.
Project Number454ACL08.
REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9%
oo
TH Corporsion
SPONSOR'S REPRESENTATIVE SusaAn. Beach
LocATNoF STUDY, RAW
FRA Rep: [DATAAND A COPYOFTHE
WiElasdtloinfeIntematMiaryanod n3a1lL6t0d1.
SLTD,LpEroDcENRMADTEORN
asaactos
TEST SUBSTANCE:
stow. NowpuaLTEST
(CONCENTRATIONS:
"CMOENACNEMNETARSATUIROENDST:EST
PFOS (Perfluorooctane Sulfonic Acid Potassium Salt)
PMRiOnSo:w(APnimeEaprhleyleLsifp-rSotmaegles)Toxicity Test ith the Fathead
NegativeControl, 0.13, 0.28, 0.55, 1. 1,22and 44 mgai/l
NegativeControl,0.14, 0.29, 0.58, 1.2, 2.3 and 4.4 mg a.i /L
`TESTDATES:
tmvomior esr: Tesoro
ExperiStamrte-nAutguastl5, 1999 `Biological Termination -September 21, 1999
Experimental Termination September 22, 1999
7D
Fathead Mion (mearlomse)
SOURCEOF TEST ORGANISMS:
`Wildlife International Ltd. Cultures Easton, Maryland 21601
AGE OFTEST ORGANISMS:
TOCRERVEDERFECT
(CONCENTRATION:
COJ NCINTRATION.-- TMoAuXIMCAoCrCEcPmTmAABLTEIOn
ges <24hoursoldattestinitiation
029mgai/l
ostmgaiL, odingain
Wildlife International, Ltd. 3.
CERTIFICATE OF ANALYSIS
Projet Number 454A-108
INTERIM CERTIFICATE OFANALYSIS Revision 1(9/7/00)
(Centre Analytical Laboratories COA Reference #: 023-018A
3M Product: PFOS,Lot 217
Referen#ce: SD-018
FC fofeme7 PurSityp: 8e6.e9%
1 Rew]
|i
Niels
_
TT
ee EEE i
32. SMoadguemsum L [ir i BEANEEE
|, Goolwa
3 nowy
i
s55. hNickael m |a E ona E l || DSe iaote enen
ET RE ee 7._Manganes Cl
7._<0.001 win%e
[[RPeusindyuablySaDvseCrs_(FGA) [J= T 2 iemE iesT n NNoeAbpplrc-abi |
L
e"Ane ions EE
ous
|
23&.. BFNlruooomrviiddee a L E E L S ] 2 e aw sim h an
Nina Bo Ee
6. Phosphate pio Eee
6 <0.007 wt/wt %
Cr7e._SuAlcfiadtse'
CE hi e a e
7.8.76 wtiwt%
|
!
5r 3 oeao | a at S a t E E ] dC 1 l ie w w
i
LE
| sae
i
2. aHydrongen
2E . TheoreticaE lValue = 0% 2 024dwihi%
i
{
3. Nitrogen
4. Sulfor
3. Theoretical Value = 0%
4. TheorVealtuei=c59a5l%
34. 818744wwitiAn%
i
5. Fluorine
5. Theoretical Value =60%
5. 54.1 wtiwt%
1
`COA023-018A.
Page lof 3
INTERIM CERTIFICATE OF ANALYSIS
Centre Analytical Laboratories COA Reference #: 023-018A DateofLast Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen 0-10C Re-assessment Date: 08/31/01
F"lPuuorirtiyde=, 01.005%9%- +(NsuMmRofimmpeutriatlieism,pu1r.i9ti3e%s,or1g.a4n5i%c+aLciCd/iMmSpuriimtpiuersi,ti0e.s,388%.t41P%OAIAno,rganic.
0.33%)
Total impufrormailltesyts = 13.09%
Purity = 100%- 13.09% = 86.9%
Potassium is expected in this salt formand is therefore not considered an impurity.
pobusrerivtebdyfDorStChisissgaemnpelrea.lly not applicable to materialsof low purity. No endotherm was
"Sulfur in
inorganic
the sample ap
anion method
pears to be
conditions.
converted to SOs
The anion result
and hence detected using t
agrees well with the sulfur
h
e
determinationin the elemental analysis, lending confidence to this interpretation. Based
on the results, the SOs is not consideredan impurity.
STFA
HNFFBPAA PFPA
Trifluoroacetic acid
Heptafluorobutyric acid Nonofluoropentanoic acid Pentafluoropropanoic acid
"Theoretical value calculations based on the empirical formula, CsF17SOsK" (MW=538)
This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160)
coAn3-018A
Page20f3
INTERIM CERTIFICATE OF ANALYSIS
Centre Analytical Laboratories COA Reference #: 023-0134 LC/MS Busty Profile:
Te e arr iT r a eeo e ]
eeT r ra Tame y|
(ETeA w 77T T07 h en e Note: The C4andC6valueswere!calculatedusingthe C4 and C6 standard calibration fcurrovmest,hereCs4pecatnivdelCy6. sTtahnedaCrSdvcaulruveesw,asLickaelwciuslea,tetdheusCi7ngvatlheueawvearsagcealrceuslaptoendseusfiancgtotrhse average response factors from the C6 and C8 standard curves
Prepared By: David 5. Bell
Date
Scientist, Centre Analytical Laboratories
Reviewed By. John Flaherty
Date
Laboratory Manager, Centre Analytical Laboratories
conuzs018A
Page3al3
ilCOdTlOiXfICeOLIOGnYt&eArNAnLaYTtICiAoLTnESaTIlN,G SLERtVIdCE.S
September 27, 2000
Ms. Susan A. Beach 3M Corporation
Environmental Laboratory 935 Bush Avenue
PS3t0M.PBaBuuoli,lxdMi3in3ng3n23e-s13oEt-a0955133
Dear Susan:
Enclosed please find Addendums for Wildlife International, Ltd. Project Numbers 454A-101, 454A-102, 454A-103A, 54A-104, 454A-105, 454A-106, 454A-107, 454A-108 and 454A-109
[LT
----
Sincerely,
:
KutR Drotar
Senior Biologist
E[ntrerse
65% Commerce Drie - Eston, Maryland 2160 el 40-22-860 Fox 410.822.0632 - El ecotoxawidiierntioalzom
European Office: Overscheenseweg 1A- 1412AA Naarden The Nethertands Tel 31(0)356949261 - Fax 31(0)356948229 - E-mail aeopolkd@woridoninnel