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362 | (@ | S| ig3zg] nna ENVAMRDQUITIINAL OOrermssatoionndoerCEonnmoisonCco otDaitpnpoedrDeecpoennes Avanos JEOXIVNITRMT OEMEETNITNo DGIORFECTTOHREACTHET EMICALS COMM= ITTEE AND =r THEWORKING PARTY ON CHEMICALS, PESTICIDES AD BIOTECHNOLOGY i 3 | cooreonErxisaTINtG CiemiocanLs | HAZARD ASSESSMENTOF PERFLUOROOCTANE SULFONATE (FF0S) AND ITS SALTS | <3 | 8] an Zo | Z=z 333 || = ao Hi aToot3sen E onan Tm AT S m a oie ENVAMRD(2002)17FINAL Preface hMInuemmthabenermhaercagoluitnhntshraioezfsartadhegsroneiefndtphetromfelieunotfrioonrogmcatolalfnyethwseouTrlfakosntkaotgFeeot(rhcPeeRrOotSno)cEtxooilslpteritondgiunCcfehoearmmiahctaaisloansrdo(2ans9s-te3hse0smeMennavtyi.ro2Tn0hm0ee0n)dteaseclviessronandl Sufesocelrleootfwaeridtahttehsaeesascinhsnteoimunignccabelysmerebnqetugbeisyntniainmngagjreioanrdi2Ul0y0S1a.mvaaiTnluhafebalecUteSuxrpaeon-rsdu3rteMhei-ntUfooKrmgalaotgbiraoelneldyfrptohomaslMeeeaodmutbtehtehrecamcoatuinnvtuirftiayecstwuiartsehsaetnlhdle as fiom non-Member counties through IFCS, An informal meeting was hostedby he US on 26-27 October 2000 (Crystal City, Virginia, US) to: +relveiamewabtohuetcaucrtrieonntssbteatiunsgotfaaksesneisnsmotehnetraccotuinvtitieess;; and + identifyplannedorongoingwork on this issue. sInumpmraerpiaerastoiofnkefyorsttudhieesm,ettoigentghe3r Mwitchirecxuploasteudreaindfraofrtmaitnitoinal assessment report including robust study BiMAoetttmehbceehnr3o1c"looJugoniytnit(e7Ms-,e1e0tthiNisnogavceotfmitvbiheteyrCs2hh0eo0m0ui)lc,daiblteswauCnsodamegmrrtieatekedeenthaaurnn,ddsetirhnectehWetoherixksiiswntiagnsgPaaCrmhtaeytmtoiencraoClhfsesumPfifrcioacglirsea,nmtPmemes,etircoeivdseers1saeane1dn PbryotghreamTmaes.k Fore. As PFOS is not an HPV Chemical, it was not dealt with under the HPV Chemieale PeAsRtdOarbSaltiasnhhdeadztaasrndsaeallstesscetwsrsaomnseicnhtedliwdsacosunsps2ois5otneJdagnrouonaurptyhte2o0Oe0Ex1cChiDnanOwgreelbacnsodiome,mefUonSrtAcs,oamanmsdepniatntfoiornmfaDtteihcoen.1m1b%eArSIs2p0Ae0cM0i.amleTeshteiesnsgOi.oEnCoADnt, his session an overview ofthe draft hazard assessment was presented. Wc"Teihcleedildvirefadef,tFauhsnadzw,aerlHdleaaalsstshteosCsaimnnecanodtrap,woraEasntverienrvoeinwsemldeynttcioCcmapenlaesitdne,cdeasDntdeudcAieeusms.bteraCrloi2mm0e0nt10s iwnecroreporreacteeivceodmfmreonmts3Mt,hatWowrelrde aaAssUsseetsshssemmeen1nt1t. wTmaheseetdiTisancsguksosFfeodrt.hceTehaTelasTsoaksakgFroFeroecrdecetohanagrtEhexeidstSwieicntrghetCtahhreeimactiocnsachlloususli(do2ng7sa-t2ah8nedrMriaenyfcoor2mm0ma0et2ni)do,anttfihoeonmrseovgifostvehedernhhmaaesznaatrrsdd and BIACon isk management activites curently undertaken or planned in Membercountries on PFO, rBteitochtoeemcmh3en4no"dlJoeogdiyntt(ha5Mt-e8heitNsiondvgooecmfubmteherent2C0hb0ee2m)id,ceartelhssetCrfiioncmaelmdidutnfdeeeroatfnhdethateuhteahsoWsroeirstksyomiefnngtthPewaatSseyceornendtoaCrrhsyeemdGi.ecnaeTlrhsae,l JPeositnicMideesssainndg eaTxvhpaioisslhubarlzeearibdnyfaoJsrusmlaeytsis2om0ne0.2n.toTfhApeeqrhufaalznuatoirrtdaotoiicnvtfeaonrremiakstuilaofsnosneaostnesmPe(nPOtFSOwSa)sshaonnuoldtdicbsoensdaulustcsetdeindwcilatushdetshciaaslutsihioonnufloidrnmeacntvtiaaoulnattriheangtgiowntaahsle oupfontdieiqnfutfeiearclehnehtmaizlsaetnrrgdytshwo(hfpoesortefhleutororxiipnceaortlfeoldgu)iocrcaialnrapbtreoodnpeccrhtoaimiepnsosauarnenddpsr.feusneTcntthileoynpanleotfglwrueoolurlpisnuanatdreeedrlscitkooeomldypoatuonndidncsflleuarerenlpcyreetshteeonxpitcri3tsy.evnecrIeyt `iconmopoculnedasr eatxctehpits utinmdeercwhiertchuemrsttahnecheaszwarhderceatnhceecmosmopfouPnFdOSmacyandebgeraedxetr1apoPlFaOtSe.d to other perflorigted Assessment activites on PFOS and issaltsarealso on-going in other internationalfor,.. OSPAR. ENVIMRD(002)1 FINAL TABLE OF CONTENTS `RSuemcmoamrmyeannddaCtoinofocnlthsuesHioAfoZnAthTseAHASZASFEd ASSSSEESSDME.N..oneness & 21-01 20 GPehnYeSrIaClOCIHCnICfAIoPrROmPoEFanTEItXCDSiOSoUFnG ......o.voovvsoowsssorwsemrerreerrroesseseesee ProdaundcUsteofiPoROnS... 1110 2211..12 21.3 PPFFOOSS--BBaasseedd PSuarpfearcePrTorteeacttimoenntAPAPDICDAItCIAONOsN.s..o.woooooroorerrreerreoroerersoorne PFOS-BasedPerfCohemricamlAaDPnCAOcNe rrr 1133 13 22 ENVrOEXPONSUFME GE00FNAC.tal 13 2222.23 224 CBPOIHMOOBDUCUORSIEHSROIINSUIIOoONrNrGcUce.IrvOcrNceereceorrrrsemssmesmsmrmsmmosssmsemmoemoosmonsermereemeeseseeeeemees 111338 2243 241 EHVUIMrAONNMBEnItOalMOMONNOHNOGTNGc.c.....oomommomssmsnsorsooroemrooseereree 1173 OCCUPAMiONal EXPOSUTES........ omnes 17 32042 30 HNOMR-AOCCHUEPRAIONAHLAEXZDOSAUTEIS .S.cooonerssomonLos.roseorsooeeneeerees1923 MetaandbPRoATAlCOKiINsEHEmS .cvrvrrooromrosrnoeeo 23 BBL1E2 33 EDASNIMaSNrERBUOUNONN c .ro.cteo meeercrcv cnemroene rsriesnr sstsamsss smmmsmsmmmsmsmo--meorseerrmrermeerneetete--ee2os323 33204 33 MAaCUlHElUeTnORHoCcmrHaenrcseorvrrrrerosmmssmsmssssmssemrmmsesmsmeereemeernoemeeeeoennereerrrreereemreeeeeeeemnene 2256 3354 36 CR OCINE OEDENOIP SCEHTcYOoNvE IeCrHsYrC nssor smmsmr mmmerr meronmnreesesenn2s 73 DVCIOPMEal TONICHY. rrr 39 3387 40 RHEDUIOAUUCHHAVIEATOcNeIGrHrYrssssr ssmmsv momsss sosonmeereeree 4830 Hazards to the Environment......................__ TG 40 Effects on Fish, Invertebrates and AIEac.........ovoomoomossoreroeeo 56 441132 42 EAIAQRTVUeEcAHtNIsEEoDPnIIUOEAUShDeErSA.QUc.A.oI.CrcOcceFcrUosIrvScsIrmSem.rsc.mem.mr.me.mo.ssosmvsnvmssosovsmssosonswoosmmonoesnesreneeoeeeeeoeeo,, 766394 4A2L.2 SAdMiPmHeInBtIDAWNCSHlongcIrNVErMeDEAES .v..ovoomossowrsorrorre 7873 4S2.I4| DAAcUDtSIocuridgieviMircarcoonrtgmarneiasmisd.ms.s..i..m.m..e..r..e..e.oro--mm--ws--ss--omrroerreern 7I7 3431 432 S EACcOtsonETerrIes0tSriCaFlEODrAgEanisms co.v.e.r.s.ovowsowoeeeeeose TERTESHIB PIAS smsmmmmeneemmosssneereereeeeeeseeeo 7799 70 3 ENVAMRD(2002)17FINAL ABE1 Annex2 Annex3 Annex Annex Annex6 ECOIOZICH SOIC corres, 89 Robust SummaroifKeesy ECOoxicology SUE cerevrevorsnvrmn 91 ACpopnlciecnattriaotnioonfsEfqourilPiFbOriSumSalPtasrtiintiSooinlinagnMdoSdeedlismteontDecterrmieniengrEffect 213 Summary of the Lowest Acceptable Effect Concentrations over... 216. ERobNusVt SFumOmaNrFiMAeLsEfESorRUPE(hySsaical c Chemico al Pror pertiem s and s 218 BRIoObMuOsNtHSOuFmImNaGrSiHeUsoIEfSToxr icology and Hur man r 256 ENVIMRD(2002)17FINAL RECOMMENDATIONS OF THE HAZARD ASSESSMENT Perfluonooctane sulfonate (PFOS) is acandidat for further work: liSsuifmpfieinrcasiitesnittoennitn,hfaolbrFi-moiaaftceicoounfmuePlxiaOstStis;vetothaaendddhraetlsofsr-iihcfaztioasrd1m0ac0mlamdsaasylisfiiacianntrisaopnse,cfio2ers0.a0lldaSTyIhseDrSienhmauormneakenspyehsce,ialeatsnhddeiynfedfapeorrisennticnse,shumiPnaFnstOh,Se aeTlphlaetagoettsoo.xniiaccliittythyoupagrnhofditlmheoerntoaeflointPaytF;eOtSmheaydiossbees-imrmioelsraperonseasnemsioctunirvgev.eraisItnsvaeadrnyddisteimeoopnnak,feo2yr-smy.eaarrtabRiietop.aesastTaehydiisneoxrcapctoussruhsraeis.nsarhneoismwulantlssthoitfn tnehexeppaostspoucerecelileustloadrifaPfFdeOreeSnn.ocmesaressiundlottsonxoiitcnoakpihpneeeptaairteotscobealenlurdlealirnatteahddeemnoopmdeaersooxfiasanocdmteiotpnhryoorlofifiedPrFatOfiooSlnl.iwciuFllaurritnhcceerrlelawsoeardkoeuntroomacablisulc.iitdyatthfeeo Ipnrceiddiecncreiosfkbtloadhduemrancsan.cerE;pifduretmhieorlwoogirck isstundeieedsedhatvoeunsdheorwstnanadntahsisoacsisaotciioantioonf. PFOS exposure and the. Siufpfeircsiiesnttenitnfionrtmhaetieonnveixriosntmsetnotadadnrdeshsashabzeaernd sclhaoswsinfitcoatbiionocfooncaelnltSrItDeSinenfvisihr.onmItenhtaaslbeenednpodienttesc.tedPFiOnS.a annudmbbeiroaocfcusmpuelcaiteisonofpwoitlednltiifael,iinndcilcuatdeincgaumsaerifnoer cmoanmcmeranl.s. It aipspepearrssisotenbceeo,fplroewsentcoemiondetrhaeteentvoixriocnitmyen1t0 oanquaetfifcecotsrgoannissoimls- bauntdtsheedreimiesnetv-idduecnlcleiongfohriggahniacsumtseatnodxitcihteyetqouihloinberyiubmeepsa.rtiNtiooniinnfgomrmeatthioodn misaayvanioltabblee osufiatapbrloedfuocrtipornedsiictteianngdPiNnEeClSuefnotrsthaensdescloumdpgaertfmreonmtss.ewaPgFeOtSrehatamsenbteepnladntest,ected in sediment downsirean aGnidveinsktheasaspepsasrmeennttwmidaeyspnreeeadd tooccbuerrceonncseidoerfePd.FOSI,n naadtdiiotniaoln,ordarteagioonnalitsextpoxoiscuirtye tionfsoorimlatainodn sgeadtihmeernitn.g edfwfeelcltisnognosrogla-niosrmssdicmoeunltd-dbewelgelnienrgatoerdgaanssiasmpsosatn-dSPIDFSOSacthiavsityb.eenTdheetreectiesd ciunrrseendtilmyennto ainndfoirsmaptrieosnenocne in sewage sludge could es1 sdoil exposureifspornagericaultudral land. SUMMARY AND CONCLUSIONS OF THE HAZARD ASSESSMENT apPrpeoprdrfulocuxotirimooaontcestliaytnees4.iss5un0lof0tocnmlaeteterai,(cPbtuFotOnts)heoarfendPisFiOpsSr-oardleuclstaitaoeend icfnuhltelhmyeicuUaSrl.isnEahuatrveoepdeboaerengndanJipacrpoacnd.oumcpeIodnurnaedncnseu.natllyyTe.haersTn(huteomb2me0a0rj0o)or,f lobal producerofPFOS intends to cease production by the endof 2002 "fTrhaectmioanjoorftithyeotfotPaFlOmSo-lreeclualtaerd wcehiegmhti.calPsFaOrSe-rheilgahtemdoclheecmuliacralwseairgehtuspeodlyimnersvairniewthyoicfhpProFdOuSctsr,epirnecsleundtisnga afsormsuulraftaicoen-raenadtminenstpsecioaflifsaebdriacpplfiocratsiooinlsisstauinchrsesfisitranfcieg,htcionatfionagms.of paper as pat of a sizing agent sPiFgnOiSfichaanstlya wsiotluhbiilnictryeaosfedapsaplrtoxciomnatteenl,yf5o50exmagm/plleinthpeuproetawsastieurmatsa2l4-o2f5PCF,OSThhaes asolsuobliulbiitlyitdyeicnrefarseeshs iLwaotgerKoofw3c7a0nnmogt/Lb.eamnedaosfu2re5d.mTgheinpoftialsesriedumsesaaltwaotferP.FODSuehats oathleowsuvrafpacoeu-rscprtevsesuprreo,pe3r.t3i1esx o1f0PFPOaS,at t2h0e s ENVAMRD(2002)17FINAL Human Health etIhnmephluUomySae.ne bitnlheoDoehdcisagathumerps,tleAsr,elpaPobrFatOmedSa (bha1ls2o.ob8de3esnpeprdmeu)t.mecltMeevedealinnotfhPeFPsOFeSOrSulmeovwefalssochcoaubvpseaetrbiveoeendnalidnarno1dp9pg9ie5nngeirnianlatphoamptaunlpualftasinocntsua.rnidnIg3n `lpgelevaneneltrsailonfpBo3ep0ul-lga5it3uimopnp,sbi.nsceIernu1im9n9dc5ia,vliltdchuceatlemdasseftrruormmecbselanomtopbdleebisnagnokb1is.a3ai2nnedpdpcfomrmomamenrdacd0iu.al8lt0sspaopnumdr,ccehsrielhsdparevceetniivinendliyvc,aartiienodu2sm00er0ae,gnioPInnFsOtSoh.ef the U.S.. mean PFOS levelswereapprosimately 43 ppb. pASenrvtoewgreraralpm.ocfocBruepleagmtipiulomon.yaelessCtruaodtsisce-saschehcatvpieloannbatel,endsitcduodnniedosu,crtebpeaodsrtoedncovononsliusttnhetenetrrsaessasutosctihaeotfai3oMnsvpbloealntutnwsteaeirnnyDwemoceradkteiurcrsa,'lAPsluFarObvaSeimllaleavanencldes al1le9ks9sa7l.tihnaeInnp26h0o0ps0pphmawthaaensned,tcheteorttaaalnianbliyhlseiirmsuabitinoncl,louagdnyed,d hAmoLarTmeonewamelpr,leoaysneidgensoitffhirceaornmtcllbyionti(chpalp<lacaht.e0s3m,)imshteiragyhnepvraarlfaoumreestwfeoorrrskteirrnisgl1wy9ci9etr5hidatenhsde, th0hi5ygrhfoeoisrdt bhoPotFhr)Om.SonTsehcbreiunamdsislnoecgviearltasitoo(n1w.wa6i9sths~iTg3n1i0f.ai0lc6saonptrplemym)la.oiwneeSrdeirsniugwmnoirftkirceiarinsotdowatinhtdyhrtpohonsiintheiivgwehaeissntsmPiuRglntOfiSviacsraicnarlbuylmehlrieegvgherelesrs(saipno<dn satnaatliystsiecsallaydjsuisgntiefdicafnotr apsostoecnitaitailoncsoonvfeorunidemres.betwAeenlonPgFitOuSdinaanld cahnoalleysstiesrolo,fttrihgelsyecerdiadteas,daindd moort erepveiadl tbahnoedishmtehapelaltcirncouspsma-brseaemcrettioeofrnsae.lmapnlHdooyrlemoeonsgnieptsaurdtiwinecarilepsanttuoidntigeisan,ccrlsouusdcsehdsaiasnmtpthlheiensvegolapunenartliayosrdeyss,.natthuTerheedoriffefetarhreeentmseelvdaeibrcsaallalnsidumriavtneaaitlliylotaninsccae(l,0 ctheecmhinsiiqruieessubseetdweteonmeemaspulroeyeseesriumthPeRODeSc,ataunrdatnhde dAinftfweerernpcepslanitns.PFOS levels, demographics, and clinical nnoanmmaolritganliatnytstcuaduys,eswhwiecrhefnooltloewleedvatweodr.kerHsofwoerve3r7.yeaarsst,atmisotritcaallliytysiigsnkisfifcoarntmorsitk oofftdheeactahncferromtypbelsadadnedr tcahnecmerhwadasbreeepnorteemd.ploTyherdeeatmatlheeepmlpalnotyefoersmionrtehetchoahnor2t0diyedaros.fblaAdldleorfcatnhceemr (h0a.d12aelxspoecwtoerdk).edanidnahligohf owefxapscoasununcrdeeerrjatoanbkdsenoftofhroearrtenlmoepnalsmoay3leiyeegsanrasw.nhthIcnaoonddwirtoirtodknoessdcewreeartreentheexfoaprmliamnnoterdbbi.edtiwtIeyneconrueta1cs9oe9dm3essak,ndsan1w9"e9e5r,peisnMoodtaenroyefpcodaritfrefd"erfeaonnratlmyyopseisss `orrfeopwotthrhetse,cdonafdonirdtinnoeenoospplloarassdmmissdoonffottthhereegaamcsahtlroesitanrttieespsttriiocnadlaulcsttiirgavcneti.fiscyTashnteceesm.e, rHtihsoekweroavvteeirroas,lwlaencraeitnehcgirogehraeyssetodfirnciesakmnpcolefrosyeepaienssoddweibstehnwtiahgens highest and longest exposures to luorochemicals NAnoimfaurlthsetrudmieetsabsohloiwsmthiastePxpFeOctSedi.s weElllimaibnsaotriboendforroamlltyheanbdoddyistisrisbuotwesamnadinolcycuirnstvhieasheeruumriannedatnhdefleicveers. aTdhuelrteaatrseasnpdec2i0es0 ddiafyfseriennCceysnoinmotlhegueslimmoinnakteiyosn. haIlnFhlufmeaonfs,PFiOt Sa.ppeaTrhsetobhael-iqufieteinansgeerru.m Ais r7e.5cedntayhsalifn spfeiaremieoandaasnlayslspyitasincnwaialnsgrucn.8on0dTuhdcaetyesmd,eaomnneah9saurler-teiidrfeedinf3otrMriPpcFlhiOecaSmtiwecaawslitw8ho.r6ak7lelrytsei.amres(PprFoaiOnnSgtsesfa2rm2polm9eesa2cw1he.r3seyuebcajorelscl,tecSatnDedal=oyv6z.ee1rd2)4.intitmhee. fLPrCFeSiOa0Stionhgafs(0s5.t2hhmoegw/snklinminoodfrreaatrbsabtihetassa.cbuePteenOtrSoexpiohcraitsteydn.botyPbteFheeOnSosrahwloaswronfuttoeounbwdeittghoenbaoetaomtxiilLcdDliySnOimvioatfrait2ei5tn1yg otmfog/atkshgse.aeyyseAysstaoennmeds-.hnoounr. 6 ENVAMRD(2002)17FINAL esNxtuepmeopesrufororuesattorsePpaeFnaOdtS-pdrorisemesautoletass.litnoAxdhivecpeiatrtysoesttosuxidigicneisstyoonfantPodxOimcSoirttyhaaolvibtesye:brevteehndecidonons9de0u--crdteaesydporinanstresattcsuudarinvedes ipfrnoicrmlamutoderestd.aliinItcnyrgeeasneevsrearf,yn aafbinvndeorramebanolzviyetm.ieess,Iwhneeipaagthidtciclvtoaascrsu,yocl2oi-nzyvaeutalirsoinobaninso,dasahsenapdyatdoiecnaethlS.lpurlaaTrghuehesy-peeDreaftwfrleoceptyhsy.wraegtras,estrrhoeeippnoatrtetosettodixnaiatclidetofyfs,eecstcsho,arh2faecmtametrgoiklzogeg/didcabayyl Hpceeinpgtamrteionlctoe.bluollrualrcaernhtycepeienlrottbirulolopabrhuyhl,erpachteyonpcteyrrtitilrocbovuplahacyruowlcaaostsiiaonlnospowhaoisblscneorthveeedpdantioncmyatmiliced.-adngodrslaeonru(l2feesp,mpalmce)enrtmartaisllegoibvrualetan.r5 hoSeirpg2nai0tfoipccyaptnte N(i0nOc.rA5e.Ea2sL.e5s.wioanrts2h2e0pippnmpcmi)idn.enfceeBmoaafsleecdyastots.nictIhhneepmapatalotechseo,lloltguhilecaLarlOdeAfgiEenndLiewnragastsi0ion.n5twheeprplemv,feoa,unntdhadeiNnLOOalAlAEEthLLe mwwaaalssen3ortpaepesmtdblaginrsdohuetpdhse syAepdorvsuecmrtsiecvhiotslyie.gsntpesrrooolsf,taattonoxdinc,ilticypoindovubdlsesepilroevntesid,onaitnirnoRpthhheyeosfuadstrhenmeaolsnsa.klievyaTrhysetgulddaionesdses iarnnacndlgutedheefdopraanntcohrereseaexsi,aemf,faereckmtesesdiwsda,escrdreieaapsmoehrsetaei,dn mbbeoetnywkoeeneydns.71w5le-oe3wk0s0fooimngdt/okceotgnriseaauytm.mpetnitNonoa,t mdeooxnscekessesyiasvsesluosrwavlivavasetdi4o.bn5e,ymloganlbkdogr/3eddawye.berkeasItnhiinantgo&,-tmhroyepanottamhcetnsitvtiuatdtyy,1o0afmtaCxeiyank,ohdmeaopygatuioscf wvaecrueoloibzsaetirovnedaantd0h.e7p5atomcge/lklguildaaryh.ypeNrtoroepfhfyec,tssigwneirfeicaonbtserredvuecdatitodnsosienssoerfum0.c1h5oolerst0er.o0l3 lmegv/elksg,/daanyd.deaNtoh ceffoecntscewenretornfoatPetRdOiSioninanitmhealsseratumafnyodlosle o2lew6vewileenfkoglsloofwitnrgeaatmSentewaks 1r.1ec+over1-53, y pe$ri8o.d5. + 4T.h6e7asnvdera1g6e0 ave2r3a.g9eucgo/nmclenftorrattihoensfewmearlees1i5n.t+9he 50..5043,,608..115+an5d.705.7a5ndmg1r9k4g/+da8y93gropuipgsi,mlreinstpehceti0ve.l0y3:, f0o.r15maalneds,0.t7h5e 4m1g4/da1y15grpougpsm, rfeosrpetchtievefleym.aAlefsteirnththeeS02.-1w5eaenkdre0c.o7v5ermyepkegr/iodda,ythgerosueprsu,mrleesvpeelcstiwveerley;21f.o4r +ma2le0s1, atnhde raevsepreacgteivceolnycentrations were 19.1 + 0.805 and 41.1 + 25.9 uml in the 015 and 0.75 mg/kg/day groups, a"tTsh.e poTtheenrteialwacsaarcisnioggneinfiiccainttyionfcrPeRasOeSinhatshebeiennciedxenacmeinofehdepiantaodcieeltlaurlyar2-aydeeanrobmiaosasisnaymailneSsparnadgufee-mDaalwelseay chtheeeplatahoidcgeehlnelosumtlaasrdaoadsneednoocfmoma2bs0iannpedpdmctahryctrihoneiodmfafesoml.alilceIunslaaradtdciet2li0oand,peptnhmoermeaawlsasosanhdaadsciagr4nciifsniicogamnniatfsiicinancnrttehaeisnemcarinelaetsheryercionoivdecfrooymllbgiircnuoleuadpr a1 20 ppm. There was no evidence ofperoxisome proliferation inth livers ofthe trated animals, rPeopsrtnoadtuacltidveeatthosxiacintdyostthuedrydienvaetl.opmAetnathleeftewcotshiwgehersetdroespeosreodf at1.6oawnddo3s.2esmgin/koeffdsapyr,inpguipn saur2v-igveanleriantitohne dfiirestd wgeinetrahtaidioannywaasfersigbniritfhicwahnitllye cdleocsreea1s0ed3.0%AolftfhirestFIgepneurpastiinonthoeff1s.p6rmingg/k(gF/Idapyupdso)sae gtrh ouphidgiheedstwidtohsien n4oduasy.s a0f.t1earnbidrt0h..4 Amsg/a dreasuyl,t owfertheecpounptimnouretdailnittoy tinhestheectonedtgoepnedroatsieong,rouTphs,e oNnOlyAEthLe tawnod LloOweAsEtLdofsoer vtheeduscetcioonnsdignenpeurpatbioodnyowfefipgrhitng (F2 pups) were 0.1 mg/kg/day and 0.4 mg/kg/day, respectively, based on "PFTOFheOalSniivcmeoarnlacsnenditnrdsaietcriautomen itfhnracotrmeaaltslheeadFtwOsit(ahinnddcolsFue1.dianngPiFmcaoOlnSstrcwoolanssc)eahnntaardlaytdzieeotdnescftowareblrPeeFOlhSeiv.gehlesQruoiafnliPtthFeaOtiSivveirn.tsthheaernurimesuatlhnteds sfFeorrrutm.h,e and males had greatly increased PFOS concentrations in serum and liver when compared with females of 7 ENVAMRD(002)1 FINAL PthFeOsSamcoencdeonsteragtrioounps. thaPnooaldeudltsliovfetrhseamFpOlgeesnefrraotmiotnohefFtlhaensiammalesdosascerigfriocuepd. shorty aftr birt had lower e`Bcxaopsnoedsduucrtoeend{0atshPeFaOrmeSseouarlntasssooaffrdtehesuetletrtowmfoie-nxgiepnngoesrwuahrteeitohdneurrrientpghreloadrcuetcdatuticivotnei;onttsohxuiisncittphyeuppsottvueidanybt,iilailatyfcowrroeasdrsie-sftaoisntrceetsriuilontng o0sfbtufendymutawedareso `cbeoexntpdwoiesteuirnoensptrooefnPatFthaOelSstaaunnddyd.tpthohasettndpaaotasaltnaapetfpafeelacrtesx(p0foiosnludlrioewciavntigathmctiolnrkteiidnnuucoceuodsnjpumunapctteSiruonrnavliwviattlrheiasitmmeaanittn.elry eaUxnrpdeosesurultrteohfiemanlyiimlirteeogd ecoxnptorsiebdutdeam10s,rdeodeuscendotpaupppesaurrvtiovahla.ve aInnycaodntvrearsste, eefxfepcotsounrepudpurviinabgililtayc:tation alone. through milk fiom sPSiergvenelirifamilicnamnatercyrhoralenesiaisnltsitchiensddtieucdaaittehesotahratetfhrbeeodifunfcgstpircoionnngsd,uinctseedru(m0 ulnipdiedrssatnadndchtohleensetornoaltaslyndtehaetshis(d3oMnoCtomapppaenayr,to20p0l1a)4y. aDletvhcoluogphmeantsallighetflfyechtisgwheerredoaslesolreveeplos.rtedSiignnsporefnadtaelvedleovpemleonptmaelnttaolxictiotxyiciintytshteudoifefsspirintghewerarteaenvdidreanbtbiat, bdoosdeyswoefighmtga/nkdg/sdiagynifaincdanatbionvcereianserastsinaedxmtienrinsatleraenddPvRisOcSeradlurainnogmagleisteast,iodne,laySeigdniofsisicfainctatdieocnr,eaasnedssiknelfeettaall tvoanriicaittiyonwserweeriendoibcasteerdv.ed.InAthNeOsAamEeLstoufdyI, megv/ikdge/ncdeaoyfatnrdeaatmLeOntAeElLaotefdSsimgyn/skogf/dmaayterfonraldetvoexliocpymewnetarle abbllosodooydowybesvicagrghvitneadglaiadtnissdcaohnsadersgfeoo,foudtSecrominnseeukmsgptatdiinaosn,y.alaonRpdeedcaiuabc,otvioeoungsanhidnhtamhiaericmnoleatya,ncaotnnesdrimbsiltneoadoldobycforduhysutnw,ceaihgsehdtwesplomlstiausnresd,etcahreneoagrsreeaxsviiiad muteekrien/edwaeyigfhotrsmwaetreemaallsotooxbicsietryvewderaet dinodsiecsate>d.S mgIn/krga/bdbaiyts., sAignNifOiAcaEntLroefd1uctmiponksgidnafyetaalnbdoadyLOweAiEgLhoanf$d asidgmniinfiisctanetredinPcFreOaSsesduriinngdegleastyaetdionosastifdiocsateisonofwer2.5emgo/bksge/rdvaeydanind stbhoeve.offsAprNinOgAoEfLporefgn1a.0nmtg/fkegm/adlaeys awnads aevLidOeAntEaLtodfos2e.s5 omfg1k.g0imdga/ykgf/odradyevaenldoapbmoevnet,alantdoxiccointsyiswteerdoefinndicaitnecdr.easMeatienrcniadelntcoexiocfitsyboirntitohnesdaoncd sAcaNntOAfeEceLso, fas0.w1elmlgfakgs/idganiyfiacnadntarLedOuActEiLonso1fin.0memagnkgm/adtaemyaflorbmoadtyemweailgthotxigcaiitnyswaenrde ifnodoidcactoen.sumption. Environment peTnrhvoeidrrueocntimiseonnctu.frarceilmHiltoyywealvnietdrle,ininPwfaFosrOtmSeawtaihotanesrobtnereetnahtemdeenlttiefceptcleyadnctleienfsstluevrpnfsta,ctheastewwamtaaegrye sallneudaddgseteodairnmedelnelatsaednoodwfnslPteRarOcehSaamtteooafttha4e S`tnautmebserhoafs csihtioewsninwitdheesUpSr.eadSadimsptlriibnugtioofnsoefvePraFlOwSiadnidfeitswpaesciedsetferctoemd invatrhieetpypobfsirtanegseaicnrotshs tphleansmiaeodf Tseovceartailonsspaeccrioessosftheeawgolersl,d.wild birds, and fish. PFOS has been detected in marine mammals at 1 number of eFPn-Fv6OirSPoanmmise3n/tpmaerloslicso.tnePdniFttOiSoinnshaatshnedbeiesennnvoistrhoenomwexnntpt.o ebioocIvtcooltnadtcoieeelnsitzder,ntoetbainsheytddhreoonltyiassnseu,seisrpowhfaottboellruyepgsaierltliotsriuonnbfciiosohedTeaigncrdiaednceatorpuf.nd<eI2rn bsblleuotewwgleiylelnw2istu0hn0fieassnht,dima1Bt5Ce0d0F.5(0B%CFclKe)arvaancleuetsimbeestowfeeunp t1o121416adnadys.401I3n cwaerrp,eBdCetFervmailnueeds waenrde dPeFtOeSrmidneepdurfaotbeed 5 ENVAMRD(2002)17FINAL "iLTnChveeursteuobbfrsatt4aen7sc,etmhsgeh/lolwowsteosmtotdhEeeCrSaftOuethfoaercaudftreemstihonwxnaitoietwrysP0piemcaieqepusahtaiilsceaso4rgp8ar.noiomseumlsEa,sCt.hfoeorflo2tw7heesmtelLi!tChSfio0urmDfoaSruplh.finsihaiFsmoaarg9na6qa-uhaaotnuidcr wfSoekrreeslaeclttoownnadetumecartcseopdsetocanituetmsh.a,e 9p6o-thaossuiruLm sCalt.valFuoeroalfgae3.6,mtgh/elpfootrasthseiuMmyssaildt gsahvreim8p9M6yhsiNdoOpEsiCsobfah-ia5..2 Bmoetlh wteistths sdLaaolytn.gN-TtOheEerCmlot(owsxeuirscvtiitvNyaOld)aEotCfa0if.so3vramaiq/lualabtliienc aifnnovrcefarirtslehybralanitdfessaqiaugaaeti3tc5es-itndvaweiyrttN hebPraitmeEesp.haTC lhees plrooowfems0et.l2aN5sOmuEasilCngffotorrheMfipysoshtiadissospais4ui2ms Dbaahpihaniuasimnaggtnhae,paostoasussiiunmg stahlet.potFaosrsifruemsshawlat.ter species, there is 4 28-dayNOECopener of 7 mo for ofAenstgtrhaoewviinhehaiib7nih-tiidboainyotifIoCnfsrtoeosntfdh1pa0rs8obdmeugce/tnlicooanrrariinnedhdieobviuittdioeonnncoePfoRffOrsoSunbdplopetrtaohsdasulicutemiffosencatlast.nwidath7-LdeamynNaOgEiCbobf(D1u5c.1kwmeeedl),baTsheed iPnFhiObSitidoonestesntotthaep3p-ehaorutroIbeCtvoaxliuce1f0orsePwFaOgSe (sploutdagsesimuimcrsoaolrtgwanaissm9s0.5Imnga!n(ancotmiivnataeldcsonlcuedngterarteisopni)r.ation spNuaorrftaidctatitaoanntianrge maovadiellasbletofodrereifvfeectas PonNEsCaiflodrwetlhiesneg coormspeadritmmeenntt.sdwemlalyingnostpebceiesa.ppTlihceabulseetooftehqiuilainbiroinuimc BPoFbOwShihtaesqbueaieln, tCeosltienduosnvitrwgoinisapneucsi.es Tofhebildo,wetshte aMcaultlearddiedtuacrky,CAneasvpalluaeyrohfyn2c2h0osm,gakngd tohfe fNooordthwearsn wdaest.erimnicnoendtrianstt,heobtaesitnewidthintthhe qtueasitl.witThhtehelodwuecks.t NOECof 37 mg/kg of food for effects on body weight `PTahFdemOriSeniasprtoeetradesadtsaivuiamavatsiahlltea.sbeleTrohfuertseoesm.satcTuudhtieeesoarciaunldtieacnaodrtaeclomntotedsaetcrtyaitteoelxdiaecniddtyahei7sg2th-shooorundretrLhseoDHftoonfxoiercyiitbnygeeesot(fiAoPpniFsOomfSePltlRoiOfbeSeroeas)fww0ih.ne4gn0. 9u6g-/hboeucraNndOEaL72o-hfour1.9N3 iOg/EbLeoe.f0.21 ug/bee. The contact test yieldeda 96-hour LDof 4.78 ghee and a (s"9Thh6oe-whroePusrFulOtESsCiopgfota=anss1ai2mu.p1mhismabglit/a)n0,btefearroacgtuotgeeemlnbeyrsyitososx.sictuT0dhye(c9am6r-irhinoeiudmrouLumtCcswoint=che1nX3te.r5naotmipgoun)s,tlhaaaetnvdiisncha(iuAbsfieitecmdaanlgfrcoolrwamtwahetdioofnfsrtohgie)n s9ep6me-bchrioyeuosrsLwaCs dtoettheerm9i6-nheoutor bEeC7,.9i7nmdgi)c.atAinagteroawtogpeontiecntiinadlefxorofPF1.O1Sw1a0bsedeatdeervmeilnoepdmfernotmalthheazraatrido iofntthies 9 ENVAMRDQO0DI FINAL 10 identity Chemical Name: Perfiuorooctane Sulfonate ThahveeptehreflfuoolrlooowcitnagnCe AsuSlfnounmabteersan:ion (PFOS) does not have a specific CAS number. The acid and salts aacmimdo(n17i6u3m.2(3N.1H)L) salt (29081-56-9) dpioettahsasnioulma(mK'i)nes(aDtE2A7)95sa-l3t9(-032)25-145) Tihium (LI)salt (29457-72-5) Molecular formula: C,F,,SO, Structural formula: CF CFsCF-CF-CF-CF-CF.-CF-S(=0)(=0)0 Synonyms: 11-1O2c.ta2n3e3s,ul4f8o5n.ic56a.c6d,.717,.18,823S.-3h4e.p4t5a.d5e,c6a,f6l.uo1r.o7-,18-.o.cSthaenpetsaudlefcoanfcluacoirdo:: II--OPcetrafnlcusourlofoocntiacneascuidl,fohnecpraacdied;caflvoro-, HPeerpfaltuaodreoc-anf-loucotraon-cls-aolcftoanniecsauclifdo;nic acid; PPeerrfflluuoorrooooccttyalnseulsfuofnoincicacaicdid: 1.1 PhysicochemicalProperties oCDcoutneasneoqt0lu/ewnthatetleysr.uraftnuhdcee-vahacernticvoeeu,sprpaohnpyerstinic-eooscchtoeafmnioPclFawlOatSeprraonp(deKrttoihwee)s te(speta.rgpt.riotitbooincoocloconiectfsefenlitfc,aietPnitoEnOcSafanfcntooorrt,ms bseto.hirledeeatdlesaroymreiprntseidoi.nn ccKoanenolnwionct.iebnitet)me.astywihmniaoctthedbc,eaananpupdsruoaaplrcliaylactbueeflaoetrsepdtrie(mdeaistctetidimvaftoepreudc)ropnlovosegentsKi,oo.wn2a.lc,aobnringooactnoinbcceecntotrmuastptioeodu.nn.dEsSvutetunidliiifeztsihnogenKllooagwboKreaqotuwaotriyworenstr.ee iprnodtieciantse. thIanttPwoFOstSuddieose.sPnFoOtSbwioacsonrcepeonrttaetdetoinhatvhee alimpiedanfascotliuobni.lityIonfst$ea1d9, mt Ltenadnsdto$7b0inmd g10 icnerptuarien sweaatwearteatr 2at4-2225-2C.3C,Soalnubdil2i0t.y0dmegcr/eLasiensasi3g.ni5f%icNanatClly wsiotlhutiionncraetas2e2d.2s4altCc(on3tMenCo(m1p2a.4nym,g/20L01i3n).natuInrala r2e0l0a1tbe)d. stuTdhye.sePFdOatSa wsausggersetporthtaetd afonyhaPvFeOaSmdeiasnchsaorlgubeidlittoyoafw5a6t.e0r msogu/rLceinwopuurledotcetnadnotlo(r3eMmaCinom1patnayt mpdaeertdteiirucmumil.anteeuntmlheeastpsereritseitnhceaedmsoaotfrePbrieFadOlSownotinoulspdeadrtuiilmcieumnlatatsteeflmryaotmeenvrdarouirpouasisnsliotmchiaeltaistoeenddsimbaeynndto.rtghaenFibusirmntsdh.ierngIsfptouPtdFeyOntSiisaludonofdcsePrFbwiOanSyd ttfooo. Sediments, "1T9h9e9)available physicochemical properties for the potassium salt of PFOS are as follows (3M Report, MBeolltiinnggppooiinntt:: n>o=t 4ca0l0culCable Vapor pressure: 3.31 x 10 Paat 20C (3.27 x 10 atm) 0 ENVAMRD(2002)17FINAL Airwater partition coefficient in pure water: 0 (<x2 10) SSoolluubbiilliittyy:: fpruersehwwaatteer:r:$37700mmgg//LL.. SSoolluubbiilliittyy:: fuinlftielrteedresdeasweaatweart:er2:51m2L.4.mg/L R"Tehpeorstp.ec1i9f9i9c):gravities (water = 1) and pH values (in parentheses) of the PFOS sas are as follows (3M DEA NHL ~~L111(7-)) ktKi.. ~L0i5e0s%)) cTahleculaabtoevdehewraetine,rustoilliuzbiinlgittyhevavlaupeosr pcroersrseusrpeoondfto327txhe10follaotwminggivHeennirny'thse L3aMwRecpoonrstta(n1t9s99():aman), FPruersehwawtaetre:r: a370x513010" FUnifliilrteedresdeasweaatweart.er: 2144xx 11007 45. pointofreference, the Henry's Law constant for pure water at 20 C is 4.34 x 107 20 General Information on Exposure m"Tahneufsatacrttuirnegdmattherroiuaglhfoar pPrRoOcSe.srselkanteodwnchaesmiScialmsoniss pEelrefclturoor-oCohcetmainceaslulfFolnuyolinfaltuioroinde(E(CPOFS)E)i.n wPhiOcShEains oelcetcatnreiscuclufrorneyn!tfiluopraisdse.edTthhreouEgChF psroolcuteisonorefplaancheysdtrhoeucsarhbyodnr-ohgyednrofgleunorbidoendasndonanmoolregcaunliecsfoefetdhsteocokrgoafni1c rfeepeldascteodckwwiitthhccaarrbboonn--fflluuoorriinnee bboonnddss.. TPherefTEuoCrFinaptrioocnesosccuyriesldwshebnetwlleenthe30c-a4r5bopne-rhcyednrtogsetnigbhotndschaairnc si(stnmonirogmthatla-)cphPuaOrienS,chhoaemlmooilcnoagglwu,eisbtu;aht bviranarsnitcachbaleded-amcihmxaitixuntruerpoeefrbfoylfpuroirosodoamuleckrytslsaafnlndudorihimodpemusroiltooifgesuv.easTrihoieunscoluuctdhpiauntiognfhltiehgnhegetrhEsC,aFnsdptrrloiogcwheets:rs hain. branched, and cyclic perfluroalkanes and ethers; and other byproducts (3M Company, 20000) SAistcactthoeersddoifoanmpgipntraoonixtnifpmorarotmdeaultcyieorn1,oa8fv4a8PiOlmSaebFtl.reic0Itnotnh1se9o9Uf7.S,P.O3SEMFn.vrierpFooonrrmtee2dn0t0ta0hl,e P3mraMontueffcoatrcicotncuarAsegtaoesrnvcioymlpu(omUretSoaEtfPiAo)1n.,i8n23t0oMmteChteorimUcpnitatonenyds 2Bm0ea0ln0gu0if)ua.mc.tuPrr3eoMdduceotsritioimnmaptooefrstPeaOdStoFitnabtloygl3tohMebaliUnPeixtOpeSedFctSepardtoetdsou.cdteicAolfnineeorft0a3c76ceor5uontbmiyentgtrhiefcoertnod3nosMff2oor0pe02r20a0tai0son(Is3MMinscCaAolnmetpswaebnarycp,k, the production POSF-derived chemicals (3M Company, 20004). rmSeioanlcceteiofmnroassctutisoePndOotSfoFthpiresoidennuctcoierrepPopOroaSltFeydmdeiernrtioivnahtitihgveheesfridnmoaollneopctroundleuaccrets.wseairHigolwhyeppvoreloryd,mueciressp,huoirtueclodpmrbpoderuicnstoest.sednTtyhpatilcpaholreltyyi,soenc1oo-nf2d9atrohyef ithneterfmiendailatpersodthuacttariescacrormipedrifsoerdwarodf iuntnoretahcetfeidnalorpropdarutcitalalyimrpeuarcitteidesM(u3oMriCmoatmepdansya,rt2in0g0.0bm)aterials or n ENVIMRD002)17FINAL irTenolfaodtraemtdea,tci3honeMmfihocaraslmsanontusfipanrccoetvuirideneirdtsiailontfhcoerormmamttehiraocnnia3olMniztaohtuetistoointdaelotvcheuermuUl4na0i.ttievydeeaSprtrsaotdeausgcoth.iaosnaPlvrsoeolcuibsmeeeesnoprdfiofPdiucOcutlSitontoorvoPobFliauOimSne.-. il3anMrf.goerhmmoaawtjeiovorenirt,yporfhoatvsoidtaeasdlsergbtlyeodbaOtlhEapCtroDitduicmtetihmoenbdevoromlicunomauennsttr(gile3osMbafCloorpmrpcoaodmnupycae,nri2oe0f0s0Pl)Fo.OcSatTechdheeomluiitcmsaiiltdesed, prhreeosdpouUncnstiiitbolenedvfoSoltrauttmehese Supports this conclusion. cAaarspeiadcceiotmfypraoinmnictlehusedeUthnHaiattleyhdaavSntedatJbeaespeaannn.didBTeehnltegirifeiumemd,ayiontahlvesaorrbiOoeEussCoDcmhMeemeipmcrbaoledurcbtuciyoeournn'tisinegnsuoitndhe-tsOrEaesCpoDrotfcefodeulrnyitnrhgiaevPseFOpFSro-oldelulocawttiienodgn MCcihhteecmmaiiiccaaSll.sABf.uoyreorfssalaeDliyr(aeDcnitrdoercyDt,or2iy000oa)f. WnIoTnhriikls&dipnCCfhhopeermmmiiaocctaaiollnns,PhraIonscd.unocoeftrJsba,epean2n0.c0o0r;roCbhoreamtSeoduirncdeespenUdSenAt,ly,20e0x0c:epOPfoDr `OECD membercounties + EMintieCnhieSmpSyAn.th(easliys)SpA. (aly) ++ MDiidaonriippKoangIunkka&CoC.heLmtidc.aGlsa,paInc). (Japan) + + TTookhykoemKaPsreoiduKcotgsyCoorCpoomrpaatinoyn,(Lsdp.an()Japan) ++ FBluNkFaLCFhleumoircoaclheCmoi,caLltds.L(uS.wi(tUzneirtleadndK)ingdom) Fluorachem Lid. (United Kingdom) Non-QECD counties + CMihlaenngijaiaAnggrCohCeimeicnacliaPslaS.nA. ((CBhriaznial)) -+ ISncdioefmiinfeiCchIenmduisctrailalCAosmspoacniayt,ioInnc.P &(InMdiaL)d. (Russian Federation) 21 ProdaundcUsteoif oPFnOS. "ifTrnhatechtemioafnjiogorufirtetyhfeoofltoltPoaFwlOimnSog-lttehlciaustlseaerdctiwcoehnie.gmhitT,chaelPssFeaOrpSeroehdliuagchttesmdoclcaehcebumleiadcriavwliediaegrdhetiunspteoodltyhimrneeearsmvaairininewtchyaitocefhgopPrrioFedOsuScotfsr,vecpa:rsessheonwtans + PSauprefarcperorteeacmtieonnt,sa,nd + Performance chemicals chEoaamcphionoufnmtdah,nesyneoctcaaPsteeRsgOotSrhieeitsspearlnfo.dductLhteeisosanstshvoaocnil9aut1meedsme3trMreipcrpertsoeodnuntctotioftoPanlFOvsSoolliudamnemdeiitssidcessactlrosinsbaerodefcinMolmmumooerrreoccidhaeeltmaiiizcleadble-lacosown.ftianiiNnsoihtneegd products (3M, 2000). In addition, varying amounts of luorochemica residuals (unreacted or partially reacted starting materials 2 ENVAMRD(2002)17FINAL (oEr3CMiF,ntm2ea0rnm0ue0df)ia.actteusr)ianrgepcraorcreisesd. foTrhweasred irnetsoidfuianlasl aprsooduhcatvseatthceonpcoetnetnrtaitailontso dofeg1r2a2de% oorr lmeestaabsolairzeesutlot PofFhOeS 2.11 PFOS-BasedSurfaceTreatment Applications a0PpFpOpaSer-ressteonlaaanltdeadlpepacathrheeerml,icafanbldrsihcpo/rumopedhuoFclusertdneirfsyoh,rinsagusnr.dfuccSaeprpetectri.efaitcTmhaeneptpsleaipacppalptiliciaoctnaitoiinnosntshpirasorvueisdeuencdaseotriet,gaookreiyn,iainnncdliuwndaedtpeirroatreecsstieistottnainnocgesf abPnFydOcSau-usrtteoolmmaoetbreisdlescuhicenhmtiearciaolrtssebalryeetahmleislgolesnu,esreeasdlthipneurabfittacenrnomerarripkerseo,tfefstisrnieioasnthamelresn,atppfoilfbiecaraptppoarrrosedl(uc3aeMrnsdC,oTamenaptdahencrya,r,puep2th00om0labsn)eu.rfya,cItncua2rre0pr0es0t,,, atpheprogxliombaaltelpyro2d,u1c6t0imoentrvicoltuonmse(3oMf CPoFmOpSa-nrye,at2e0d00bc)hemicals for this use category is estimated st 2.12 PFOS-Based Paper Protection Applications crPaeFtsOieSsg-totarenycleaitnteocdlpudacepheefmoiaocdnadlcospnatppaercrtobdoauapcprelddicaasftoipraorntspoa(fppelaartesspi,rzoiftneogcotadigoecnonnttaafpiopnrlemirucsla,attiibooanngs.s, paSrnpodevciiwdfreiacpgssr)pe,palasisec,awteoilfolln,sasainnndohni-wsfaotuoseder bocfoangtssa.icztiwnragappapslg.iecnaatntsdioinossthu(enfrdoelprdrtioandkgueccntasrmtafoinrsno,lmycpobantpyaeirpnaeaprnesdr, cpmaairlpblesornbaloneadsr,sdft(oor3msMso,mCeoamnedpxamtanesynk,,i2cn0og0np0va4ep)re.tresr)sT.nw2hT0o0h0em,aatnphpuelfiagcclatotubiraolen mpreotdiucctoinons (v3olMumCeomopfanPyF.OS2-0r0e0lbt)ed chemicals for tis use category i estimated at approximately 1,490 2.13 PFOS-BasedPerformance Chemical Applications `iPcnFdoOumSsm-terrrieacllia.atlceiodzmemdcehraecsmiiafclian,lissahnedidncpotrhonedsuucpmteser.rfoarpSpmplaeicncicafetiicocnsah.pepmliiTcchaailtsioccnaastteeiggnoorrtyyhisianrccealtueudgesoserdvyairininocualsudvseaarlfiserteoyffioPgfFhtOsipSnegctihfaaoltaimzaser.de ppmhhioonttioonlggirtaahpnohgdircaopiflhiyw,me.leldleescnuttrruforanecitccalncetha,necmaricsci,adlssmh,iasmthpyosduorpsapu,rleicschseamnfiltucsiadlfoaridndmtieettriamvleedsi,paltaaetlsik,naglciaonnaetdicnelgleeaacndtedrriostn,iivcefscl,iooccrhainppgeorlibsashtpehosst,, pc(lr3eoMadnuCecrotsmi.opanannvdyo.alu2ma0en0o0fi4n)s.PecFOtOFicSitdhceihseivnmoiblcauaimtlest,faotaipoptnrisosx(iu3msMaetceCalotymepg1ao5r1nyym,iet2ei0sc0t0itbmo;antsewBdiolsailtnbagpe,pur2s0o0ex0di)m.iantfeiIlnry2f80i30g10ht,mietnthgrefigcolatombosanls " ENVIMRDQOOITFINAL Pera Solos: Mor PraCatcies EC id Fa ` pi : ll, mnt| [Si|| [Casi ica eaves pe aee t pS F E Ir r t Ee s T| reasons CherryAmmaSls nt gwen oe ellt uh f-- e fmrrs atteeens eummatin wr w =ess| | =i a ie -- a[ rte a Ha oy a [eeatma | a ENVAMRD(2002)17FINAL 22 Environmental Exposure and Fate tPhReOeSnvidrooensmennott.hydrolyze, photolyze or biodegrade under environmental conditions and it is persistent in 22.1. Voladitity 3PPMRa.OmSmIonilst.hantotsTthueidxsyp,eacsthtseidgHnetmoewnvatoslwatatoisloimzseam,adleblabstoyedmPerooanfs.uarDneoneaasxlspiedgrniMemadecnktsaailyrlaybtuuesseridnpgoaprntrietcxipowenarticemoree.fnftiTachlieedntoteoaafpgree<ntcar2taitxoend1bb0yy oiMtuasrcfkonnaoimycenmsacatltuearste.u"r(eT.Ph"FeOSsT]hiimisps ltedheuetsxepersemsdieninaettniitaolinlsy (ins0onai-nsvsoailgganrteiielt meaefnKrtaomwwiatoqhfueetorhoeu,sHseion.lruytii'sosna. LtayTpwheic2oinissnptvraonoltbaoialbfelyc3h.be0em5ciacxuasle10oinf pcaolscsuilbaltyendehgleirgcinblefovrolpatuirleityw.ater. Overall the conclusion is that PROS is a substance with very low and 22.2 Combustion cp"Tarorobcboeensg-sisnuwlatfsourutnhbdeoernrmdsotdaiynnndatmthiehecappleolrtyfelntmoioradoleolcdetedga.rnaedTashtueilofmnoonsapttaetphemwroatlyiensceunfltoerfipinedriafnlgforoiifntathteewderaeckphoermbtioecndadl.asn,alTtyhoseisvinaicsliintehdraatattithohene `PmcoFodOmebSlussatnipodrnedtibwcyto-ipoprnoo,ldyulmcaetborsriactthorproryuogdchuocmtabufrsoatrnimgouelnaotfsitoutndesimepsaerraeotnubreeaisn.sgercioTenshdeuoscfetepdde.artfalAuwonirlolaaslbskyeelssimcmepoonmrtptoawunintlldstob.ediemntaceldrumediinnoegf whether PFOS may cnter the atmosphereas resultof incomplete combustionof was 22.3 PhotolysisiOxidation dPeFgOraSdadtoieosn onfotPFaOppSe,arEAtFoOpShEotaollcyozheol,(HMatcfiFeOldS,ET.alc2o0h0o1)l., ESFcrOeSenAinagndstuMdiFcsOSonAtahse waeqluleo2uss3 pshuortfoalcyttainct paPenFrdOflSfuooarwmoaeosrctpanrnooetdsuudcletf,toencaaltlteeda,pdp(ieHdaatrpfehideolttdo,oduTen.gder2ra0gd0ooe1,ifnod3irrMmecPtRFpeOhpoSotro(tl3yMsNiosR.etpooWr2Ft7O,7S51A)99,,9)P.FOOnAe, aprhoydudcrti,deaanndaroolmeaftiincs 22.4 Biodegradation sdTuehbgesrtabadinaoctdeieog(nria.do.fa.buiPllFtiitOmySaotefwadPseFgOorbSasdeawtraivsoende,xialnmoi2ns8oesddatiyonst,aalciMotrIhgTeaLrnIi1c5tecsantretb(oKonuxr(yuTgmeOenC:Ladanebomotarhanetdorrfwyra(oy2m00td2oe)g,mreasNduaotriesoingunlioiffimcafaetnaett cbdeaigrorbdaoednza:rtaiadonantd)i.o3n%oarfbtyelrols2is8qudiodafycsphwraaormscanottbo_sgceroramvppehodyu-tnmodabsesid0sep%neticbttyyroom(xepytrgrieymna(rdLyeCm-daMenSgdr;a;dma6te%iaosnub)ry.esrepmoArvivamelaroraygfedteogtpraelardcaoteringotanan)gi.ec oO"Txfhyetgsheeenvtadelsetumewasanasdreecixoncndeifecidanteeidvdethboefypnadosesgsricagrdniiatfteiricioaonnntoofdfe4gtr5hae%dactaoifnottnro,l7wisdtuahbyissntaatnnhcedea6ca0cniu%lriaancef,ytrlfio1mr4itwdsfhaoiyrcsthhrei%sspeotcefts.itvheelVoyarle(it7diic4ta%yl and 5%oftheoretical a days7 and 14, respectively), 23 Environmental Monitoring aSnMdSlaMnudlfitli-lCeiatcyhaStteusdaymprlepeosrtteadkeonninPsFiOxSctcosnc(e3ntMr,at2i0o0n1s2).froFmouwratoefr,thselucdigtee,s s(eDdeicmaetnutr,APL,OTMWobelfeflAueLn,t is ENVAMRD(2002)17FINAL suCioogrlnoiufcmihbceaumnsticlaGulAosr,:octhPweeonmsioacfcaoltlhaaectciiFtvLii)etses(w.CelrAeecvreolssausnpdpallTyNci,tciiePtso,erstPSOtt.hTatLWucehifafevleuFeLn)mtacwnoeunrfceaecncttournratitrniooglnsiorirasnignetddhuastftrrdiooamln0o.ut0s4en1avo10ef. `r5wa2ant9geerpapwbna.gsenTowhnae-sdPentoOencT-tdWettoescl0tu.d01g06e03.(1pdp3rby8;wpttp.hb)e;raltahnnegdfesieldlwialmseeanlctehsaastnetghreaannwg0ae.s2wnuaosng-dkneotnet-ocdte3t1,e1e2t10.11p03pp5b5p.b1t(hpdprdby:rianttkh,iengasunwrdfaittceheer. oqiufniteettrhmeweadatibeaortveaeningraetnhgeweiarss,ranenoxgnec-sed,pettaencfdtort0hteh2e9qu3PieOptpTbw.WatTeehrfefslaucemonnpttlreoaslndactitsPileosurdtsgaSemt.pflLieunscdiigneeg,nsewrhafiolrlcyhCilwneehvraebeliattnehdde,thhiweghhilesoctwh.erwee$nrnde Estfafteecst tChoantcaelnltrtahteioPnFsO)Sfosruarqfuaacteicwaotregranciosnmcse.ntrations are below laboratory-derived NOEC. (No Observed ds"Toahgmesp.lMecusaltttfi-askChei,ntayfnrdSotgmurdgoyruenaedlnbseobeeiafnncs(l,3uMadp,epdl2e0as0,1mapa).orrkkeMtmeuabssacuslrkeea,tblcseoawmqupamlniitlnikgt,iecoshfiocPfkFePOnSFmurseOctsloie0dS,u.e8c,h5iin2cknaesnto,ctaglwgseo,rfebrofevoaeudr,nd2h0oi0tn bfaoluranmcielokftshaempslaemspalneds wointehgmreoausnudrbaebleefPsaRmOpSlew;eroneeoffrotmhecitfeosurwimtihlkflsuaomrpolcehesmwicaaslfmraonmufaaccotnutreolorcistye. the AGFrliocertsiiydca.r(eSpCpoairlttisefbdoerrtnghieean)ra.ensudalntAdsloaSfsaakbalgneloIUbsSall.anmdcooniainsttCoaarlnianwdgaats.eurrs,vPetFyohOefSnoPwrFatOshSerdneitneBmaclatteridicnieSnemat,haemthImevaeMr:edaitnthdeerybrlwaoenoredeanolfoScemaati,ednthieen dmoalmpmhianlsfrformomFlomroisdta; Bali Sea ltochaetiloanrsg.est Tbhleooldarcgoenstcenlitvrearticoonncweantsra4t7i5onngwiasmL 1i5n20rinngglegd, sweeatl fwrto.m itnhebontolretnhoersne S"feTrnhotem0ptlha3esMmU.aSf.oofrFaipnsiahslcayisnvidosrWooiufsldP(iFffOiesShS-eltreivvienlcgse)((b3idraMdtsCowofamlspivasenaryms,palm2e0pd0l0eaisn).wtahsSplneaotcteile1is9st8se0ad)m',paalnneddd ctfahorerlsyePFs1Oa9mS9p0lp5elsoarswmeoabrielaeilvaneteledsr insPpcPelbc)uideuespdrt1a0hn.eg2beadmlafdxreoiamgmlbuee,mlaoolwfbatt1hr0eo4s7lsi,mpiaptbn,odfdspecrteaesecestngtileoi.nn uaPplbtaaosldmaamealagexlveei.lmsurLmaenvoegflesd20fi5rn5otmhppebb.ellveorwstsheamlpimlietdoffdreomtescixtiboi(rn1d GEiuersoyperaenpocrotuendtroines,PFtOhSe iNnorftihsh PaancdifficishO-cactainng awnadterAnbtiarrdsc.ticFilsohcatwieornes s(aGmipeslye,d concentration was 923 nglg, wet wt, in the muscle of fish from a Belgium 2f0r0o1ma).the estuary. UT.hSe. The hciegrhteasitn highest ftciorsnoscmueenttohrfeatfNiiooshnrtfohrfoPPmaFcisOfeiSvceriaanlndUi.nSAl.natnGadrrecltaaitkcesLoacikeneansMsi.fcihshiFgwiasanhs-Uc2.aS9t.i7ngdnigbdi,rndowtseactmonpwtltae,isniwndeetrtheeectcmaoubllslecelcPteeFdoOfSf,crnaopom.rtdhieMduUs.cfSil.se.h qPiunFcaOlnuSidficinoagnbcMleinttdorwa2at2yi2o0natsonlcglo/,lmlhLee,ctweBidatlhftiracommanetdahneoMepfdlia3st3m0arrnoagfn/ambLna.lSdeeaTashg,elJelasaprangnesettsheePFamnOidSdwKceoosrnteceaennrtrcUaotSaisAotnsri(anGnihgevesedyr,fsr2a0om0m1p5ln)eo.st Nforrotmh UPSacAifibcirOdscewaans a1lb7a5t0ronsgs/egs, birds fromthe Mediterancan and rwaentgewdt. ffrroomm3 a0Br3a4ndnigs/mcLo.rmorPaFntO,S Baltic Seas were reatvely less than cPoFncOeSntrcaotnicoennstraintibolnosodin those found in U.S, birds. otrhelisveetrss ooff aGlilessyamrpelpeosr.tedwiotnhPtFhOeSlianrgmesitnkcoanncdenrtirvaetiootnterofliv4e8rs00frongm/gt,he wUeSt. w(tGi.esyf,ou2n0d016i)n. mPinFkOliwvaers. fouPnRdOiSn concentrations in iverotter livers from Washington and Oregon states ranged fom 34 0 994 ng/g, wet wt tGhieesUy.S.repcooratsetda th$a1toPfFO77S swaamspldientgecltoecdatiinoonyssatterasccoonlcleencttreadtiinonthreanCgheeosfap<e4a2ketoBa1y225anndgG,uldfroyfwMt.ex(iGciooyo,f 6 ENVAM/RD(002)17FINAL 20014) dGlioocewasntysitornresepaoumrptsestdarmoepnalmitnhaegncdloocndactoeiwnotnnsrs,aitrtiehoaensmtowofofPctFlhoOesSe3tiMntosfutcrhifealc3teyMwaafttaecriD,leictsayetdhuiarmdeAnPtLFsO,(ScGlisaeumsrsyf,,aca2en0d0w1a6fti)es.rh ccooOlnflceecntttheredattfihioronemse (uswiggent:ifwii.chaenmtelnyaenagrsreesa9tt3etr0wtohuaglnokct,ahteit1on2ws9o9huaupdsgtksreeedaivmsm.esin0tte.s18c((omensectae.n)ntasronadtfi0o.1n95s50)s.uiggC/nilLfaiacmnadnatln2yd gufrgie/sahLtesrva.mtph0al.ne0s0th9wee(reuseptsctoarlneldaecm0te.sd0c5aa5t wcwoaonscel1no3tc3ra2atitouinogsnk, .foonreTthhuepesuaipvrseetraramegaeamnPdlFoocOnaSteicodnoonwwcneasnsttrr5a9et.ai1moonufginltkhceel(a3wmMestaftwctti)hl,ey.uwphsitTlreheeatmhaavleorfcoaargteitohnefiwdhaoswwnhso15tl.re6e(baeomsdt)ylowcPga/RtkiOeoS:n hat for the downstreamlocation ws 14.1(est) ugg. DTHeanennsecesnsaAe(etL20(uR0ti2hvr)eerr3eupMpo-rftaeacdnildictodynomcweennntstrtiarotneieaodmnsaobofofvtePh)e.FOTouShtefamrleelawsefurrroeemd2t0fhersoafmmlpuslouirrnofgcuhcseeimtiwscaaatlebromvsaeanaumfnpaldce2ts0urtsiaiknteegsn bfafecrlioloimwtytthhaee1 cpoouotnifncatelnltarplapotrciaootxniiomno,aftPeslFpyOacSseidwx aasmtil3ae2ps2prbo1ex1liomnwagtLet;lhyethoe2utdfmoailwllne;sttirhneetearmavvacleosr.nacgeentUrPpasFttOiroSenasmcwoenocrfeenttohrbeaatcirfoavnceidlfit1ryomitnhcetrhaetaavseeproaaitngste ntdhotewhsneeswiarroeeaasrmesgwaimaopsnlse1ds1.u4gg+e1st9sntgh/eL.absTenhceeroefpeoirtthsetratmesajtohratetnhveircoonnsmiesntteanlcysionftkshoerPaFddOiStocnoanlcesnoturracteisonosfwPiFthOiSn `lTichmoeimtp3oofMndeenEttneovciftrirooanntmc(eh1noStwan)lg./gL)aR.abtPrlReipOvoeSrrtselfedvreoolnms atinnwaotlohyesfemtsahleoeeferPastFuspOpwSliiteihrnsmnehaaaisvduerearnabdtloelgievenevroseu,lsralitenvccerhleosoawsf,edaPwnFidOthfSiasaghbeom,vewaehlitlh(eea sethtxeuadmlyievnaeneladslyhnzaedfdPeFmPaORlSOeaSrbalotesvveeslhstohiwnees2di-x1n0toynpcgoe/rsogreflleafvteiilsoohnmfweiqautlahnftoaigrtema.utlioantI,end1t8hferaonrmadta1ct2hlnoegaw/tgs,tthurredesyep,etctytwipvoeesloyof,ftfhTieshh,feoufrTishhcremheeoaowtf t1h5e7sigxlteypes had PFOS levels above the fimit ofquantitationof 3.5 ng/g the maximum concentration was. Pa"TvFhieOanS3fMleeedv.EenlsvDiwrreyornaemnenenomttialadebLtreacibtoneredastihonrriytmhpea)ltscwoyosstudsmaupmhasnreiidzfeofrdeefdiPssF,hOfaSeneddanwhaealrdyesPebsFeOloSfowalvetvihealnslaiomnfidt9s1aqo0ufauatuigackn.tfreaedtiomantriincetsh,e 24 Human Biomonitoring twFhaoersmmaolassntoyrreeycceeeannrtts,laynPadleFytOseeScstoehfdabsilnboteoheednssmcaermaupsmluoerfsetdahreeindgeteshnceerrsiaeblerpduompbouelflaotwwiooanr,nkdebrsustumoamctcamurpuiacztheidolnioanlwlTeyrabellexevpeol1.ss.edThtoePrFesOuSlt.s oIft 24.1 Occupational Exposures pPeFrOflSuorsoccrhuemmicalelvselasndhatvhee surface protction products. prboeceenssimnegasoufrethdeseincowmoprokuenrsds Biomonitoring data are available iinnvtoolvperdoduicnts,bostuhch from manufacturing tahse fimraenupfraocttecutriionng plants in Alabama anodf and Belgium, a processing facility in Japan, and corporate offices in Minnesota " ENVIMRD(2002)17FINAL Fluorochemical Manfacturing peTlowelocytmreo3rciMhzeamtpiilcoaannlts,pcreollcoecfsaustoeersdi.naitnTiohDneescaeantduPrt,OhSeFAn-lbsaaubsbascmedaqupearnnotddluycAtunsstewddeerPgprO,aSdFBeeltoogripumrmeo,tdauhbcaoevleivzaerpirotouodsuPcpRerOodSdu.PctO|sSEAt.hrtfohruiogrmhd sm1ao9n9mu5efa(PncOt=SurF1i-7nb8ga),spel1da9n9,p7rol(doxucca=ttse.1d49in)S,Ccoartnutdmag2Pe0F0G0OroS(vnel=,ev5Meli2sn1n)weesuorstiean,gmehhaiasgsuhrnpoetedrpfironordmmuaacnnecudefaPclitOquuSri,idnegbhufptloahmnaatstoemgmarpnaulpfohayyce/temusarseiedn svs1 uporelvcuetnirilolemarenetcdreypteo(rOiploasdrset.niceiGtpeaaot,emef1to9rr9i9cm;emdOeilcasanelsneshuarvvaeel,ilo2lna0lny0ce1b.;eeOnAlrrsieetpnhomreetttedialc,inm2e2000a01n0s.; aOTnlhdseesrneandegtaetasal,ware2er00ep1r1or)ev.piodreEtdvepdinlfoToyanebealse phMlieagnahtensr sitnehra1un9m9A5PnFtrOwaenSrgepldevlfeelrvseolmhsa0vd.eu1r0bienpegpnemdvee1cr0lyi1ns2iu.nr8gv3eaiplplbmao.ntche Tpplheaernitorsda.snignecTeohfe19PP9F5F.OOSSDlelecevavetellussor fisnwerowuromkreklerervsselisnachr1ao9vs9es7 bbweaeosn. s01u.-r13v20eip-lpl9ma.n9c(5erapnprgpoemg.=ra0m.T0(h65-e21l1a0or.ugt0e6sotpfpn$mu4)m0.beeMmropslootfyoefeestm)hp.eloeyTmephelesomyaeeteasenaacsthebroputlmhanfPtaFcpiOalSrttiielcseivhpeaaltadedPtFihOneStDhesecear2tu0umr00lpelvmaeenltdsitwchaaalst were <6 ppm across all of the sampling periods. tmxhecedeiepcmatpflloomryotenhieetsoywreieanrrge2po0r0f0of.gertarhmeafpmarreotdmiicciwaphlaitcimohonntiahtteoseroeifndgatthpaerwotgeorrtaaelmd,neurbmiubvteeidrt wiofanspootnteocntlteipaarrlolvhyiodeewxdp.moasenItdyweoamfsptlrhoeeypmeoeratscetdiunatlthlahtye wrveooplroukrnettdeedeartfeoderamctoohsptpalraonttfi.citphaeTthese.urrevfeoIirnlel,aadndictietsihyoeona,urlsd,agbaeendonfoittteihdsetuhneacmtlpetlahoreysheeoesdwatamanadonnyldouyrfpatrthoieovnidtoeotfaalesnmnupamplbsoheyormteoonffthemewpelrsoeeyrenueomst levelsofparticipating workers during each sampling perio. ebInmlpooolrdodye(erOeltssoefanedrdtormaelst,shet1h9De9e9c)va.otluuLrnitpmaliraytnetndawjtaousrbeoicnhffoostrehmenatmtiooonnmi,etayoesrauirnrsgewpsorerovkgeernda,dmiafanftdrtebhneitk3liMunogrpollcaohncetar,tniiaocnarlwasenrdienomccomslplalemocpytleeedcyto'of arpnardonvdi7od6mefsaialmmbpepltlteaenrctounwnsoidrsektreesdrtsaonf(d6i10n5g7raconhfdeomthmieclaydlicsphtlorasinbetuntwioaornnkdeor1fs6f(vl1ou2lou6rnotrceaehnredsm)oi.mclaylThcseheorlsueemvnelwlseovirenklestrhsiensaeneemdmp6pl1olyoevyoeelseu.sn,icweThrhose.) p(warerartnigecei.spaan0mt.ps0.l9e1d-G1ie0no.m6lea0tt0er)i1cf9o9rm8e,tahwneesrrewaensrdiemoim0l.a9sr4a41m0pplteph,mos(e1.9r25e5%p9oCrp1tped0m.a7bf8oo7vr-e1.t.h1e2M6v)eofalounrnctPheeeFrmOsi,Scaalnledvpells1a.nw4t2ee4rmepplp1o.my5e0ef5sopapnamdl pt0oh.se1i3tc6ihvepemlpyim.caa(ll9tp5hl%oaunCgt1hhm0ao.dd1e1hs4it-gl0hy.e,1r6a1Ps)sFooOcriSaftleiedmvewplilstahntthnaeunmmpbwleoorryokeesfsry.seairWnshtwehoenrfkcieolmmdppialnarnteth.debcyhPejFmoOibcSdaellsecpvrleialpnstt.iionn,mwaolrekserwserien Fluorochemical Processing pBriootmeacntiitonoripnrgodudcattas waenrdessuurbfmaicetiepdroodunctwsor(kBeurrsrisin eJtapala,n p1r9o9c9)e.ssingIn' pe1r9f9l9u,oriPnRaOtSe,d cPheFmOicAalasndinDoEfHiSre ed(prmeaprwflnlouoyfrreoo(ehmesnxbaont=eh3s2up)lrfwoodhnuaocttaeir)oenwneeormteplrmoegeyuaelseausrrl(eyndex=ipno3s2ee)mdp.wlSohyeerreuesg"mulwsaarelsryaumhlaoantddlrteahwefnlSoafrgroacomhmiebmmairacanalaspgleaanmtn.edntmaSenemarpgulmeomyewenaetss `(w1i=th 3a0n)aartitthhemeHteicadmeOfafnicoef0i.nT1o3k5yop.pm.TheThheighhiegshtePstROPSFOlSevellevienl3i2npcriotdhurctoifonheemmpalnoyaegeesmewnatsg0r.o6u3p8spwpams. Is ENVAMRD(2002)17FINAL aa0tb.o0tv9he6e,7Spapgamm,iwbhairlaeptlhaentavienrdaigcealtevleolwwearsleavpeplrsooxfimPatFeOlSy t0h.0a4n paptme,ithTerheofretshueltsmoanfubfiaocmtounriitnogrifnagcpieersfocrimteedd ciAonlrtcphooorrupagothreattmheaenseatxgapeforfsisumraiennsaJgwearopsaunladt(n3aoMt3MnReecppeolsrastna,tri1il9ny99bS)te.. cPoTanuhslii,ndeyMr-ieondnnee"osecomctpuaplaowtyeieroeensa,ls"inmfoionlraeeriottfhoewrhhogosremouprh,eapdPorwFtoeOdrkilenedvtehilens reMamunpoglreooycohefeems0i.ch0aa2ld8 mp1re0oads0uu.cr0ta9ib6olneppolmre.vreelssIenoafrtchPhiFsaOngSdroidunepvteholefoip3msMeernutem,m.pwleoTryheeeessma,mepaalngeedPwFiaOnsS19sl9ie8gv.neilfiwAcalasntol0fy.0tah4se7soppcapiramtti,ecidwpiawttihitnh3g increased serum PFOS. No other dataon thse workers were provided. 2.42 Non-occupational Exposures ssPeaFrmOpuSlmesfhar(sodmabte2iencngommomseeafrascuirbaealdcksionaustrhc1ee9ss5e7in)rutfhmoerofUmt.Ssh.oeurrgacenengseertdahlrforUuo.gSmh.4o3up-to4ptu5hleaptwpoibrolnad.nadndI2n6in1-9s49m58a,plPpbnFuO(mS3bMelreRsveeploosfrithni,spt1oo0ro9ilc9ea)dl o"lTotthhseerroefdawtiean,rdeisvauipdcpuharloaxsoigrmeapotogeorlalypehdSi0ch0uldmoocananotirsosenriaonrstaahgmepflioerfsstthsfeoruodrmocneUo.raSs.n,dwcaehnermeeiscptraiolmvaiotdreeddb.i2o0l03ogMdiocaanllosrossuapinnpalltyyhseceosdemcptohanindrit.ye-sfNivioen 199 (3M Lette, 1999). These samples yielded an average of35 ppb PROS, ranging from$ t 5 pb. mPSeeFraOanSpPoiFnolOe1Sd99sf8erro(um3mM1l8eRveerpleograitco,rnoals19s9bt9l)h.oeopdoTobhlaesnrkewswaesirn2e9v.a67r8ipoppubos.olgseTohagenrdaPpahFniOcSesrteliegmvieaoltnsesdvoa3fr4it0ehd-e6q8Uu0.iSt.deoanwoebrirste.daeTnphaleeynzodevedironaflonglr Pthbe.geWohgirlaepthhiecrlaoncgaetoiofnothfethaveerbalgoeosdwbaasnk1.4 T10he52rappnbg,e of the levels across geographic regions was 9 6 $6. iPovoelebdeesneranfarloymzebdiofoord PbFaOnSk.s inEaBcehlgpiouoml h(6adpo1o5l-s)20tdhoenoNrest.herTlhaendNset(h5erpoloalnsd),s haanddtGheerhmiaghneyst(6PpRoOoSls)leaveslos (mean= 53 ppb) and Belgium had the lowest (mean = 17 ppb). (fInuHdoPirvLoiCcdhu/eaElmSibcMlaSolMosdS)ussa(imnOpgllseeshnigfchrt-opamrle,3ss2du0ir0fe2fe3r,leinq2tu0id0a.2gbec,hpr2oo0pm0ua2lt0ao)tg.iroanpTshhywe/eersleteucdrtierecsoe"snptprlaayrytainctaialpnayndzteesdmifnmocralsusPd.eFdOsSpaedcualtntrdobmoletothoredyr sdtoantoerss.paarnticeilpdaetrilnygpoinpualatcliionnicaplarttiicailp.atiOnvgerianlla,prtohespePcFtiOvSe sgteuodmyetirnicSemaeta,nsWwAe,reansdimiclhairldarcenrosfsroamll233 cplhoiimpilutdlsraetaninodannstdh(ea3id4ru.lu9tpspppewbre,rbe3o1.u0mnpudpcsbhw,heirgaehnedcro3mt7ph.aa5rnapfbpolbre,tahreecsrlpoedcsetriavlleylly3p)o.psutluTadthiseo.n.geHoom1ewteirvsiencro,tmectahleenasrupawpnhedert9hre5arn%gtehtsioslfeo1rranttchheee PreFsOtAwofergeeohgirgahplhyiccodimfefletreendciens ailn tPhFrOeeSslteuvedlsi(oesr=ssom6e3ot,=her7f0a,actnodr rI=t s7h5o)u.ldTbheendoetteaidlstohaftcPaFchOSstaunddy are provided below. `ASCmeA:erruiMmicnaPnnReaORpSeoldliesCv/reSlotss.sinPba6lu4ol5o,dbMlbNoa;ondkCsdhoa(nrOlolorstsetne(,3e3tN2Cal;m,alB2eo0ss0,t2o33n)1,.3MfTAehm;aelePbsol)ro,toldaangbde,adnOk2Rs0,-w6ae9nrydeealHrosa,cgaetwreesdtroeiwn:onb,tLaoMisDne.AdfngreoElamecsh6, ebtleo)odfobracnakchwasesx.reTqhueestoendlytdoepmroogvriadpehaipcprfaocxtiomrastkenlyow1n0fosramcpalcehsdpoenror10w-eyreearagaeg,egeinndteerr,valasnd(20l-o2c9a,ton3.0-39, "The geometric mean serum PFOS level for all locations and gender was 34.9 ppb (95%C1, 333365 ppb, 1 ENVAMRD(002) FINAL "P3T1Fh.eO3 Srpaplnebgveeflwosratsahal<n LffeeLmmaOallQeess.(4(.93T5hp%epbgC)eIo,tmo3e10t.r6i05c6--mppe3b4a..n3)f.MoarlaAelgslehmaawdlaesssigwnnoaitfsiac3an7n.ti8lmyppophribtgah(ne9tr5(%ppreC<dI,ic0t355o).r5g-o4e0fo.m3deaturnlicdsmeewraaunem APuFoOrSocwhaesmiicnalthecosnacmenptlreastifornso.m (28.0 ppb, range: 4387.2), ChWahrelnots,irNatCifi(e5d1.b5y pgpebo,grraanpghei:c l1o9c.a3tion1,6.th0e) haingdhetshte gleoowmeesttrficrommeBaonstfoonr "fPoTrrhotemlhaniPdgo.hretlsTatnhdse.ernueTmxhPteFnhOieSgxhtemshetiagsshueerrsetummdeoPnntFoOirSnvltaehvliesulesaswma(psrlae3n2gwe0as1p3p19b65-f62rp2op6mbpapfbm)arlwaeeomrdaeomlnmoeera,bsiu6or2aeddydeiaonros4r,ofle6dm7aalyleessaarasfnrdool4md mInalbeosotrsetprraepseanntailnygseCsh,artlhoettmee(ann=4)o,f confilimditeofn1c00e.0 ppb. Hthaege9r5s%towtonl(e1r-a2n)c,e LloimsitAnfgoerlPesRO1S-1w)aasnd$5M.5inpnpebapwoiltihs'a.n uPapupler(19-51.% SpAagerert.uicmgiepPantdFieOnrgSiannledavesnltusumdwbyeedrreesoifrgenypeeodratrtesod erfxeosarimdi2en3ne8cec(oi1gn1n8iSteiamvtatelleefsuwnecatrniedont1hi2en0aodnfulelymtasldaeatsga)edaevl6da5ei.ra9lby6le(vOoollnsuentntheeetrpsaal.riinc2iS0pe0uan2rtl..e Most of the participants were under the ageof 85 and had lived in the Seatle areaforover 50 years. "pliTbnh.eeargeTrohemegerrteerswisaciosmnneaaonnasoliygfsneisPf,FiOcaaSgnetfo(wrpasa<l0nes3ga)amtdpiilvfeesflrywea(npsce<31.0i0np5g)peoabsmse(ot9cri5iac%temCd1,wi2et6h.f8oP-ar3F3mO.a4Snl).eisnaTsmhneednrfabenumgtaelnewosat.sinI3n.w4so-imme1pn7l5e oTrhbe. meanof the 95% tolerance limit for PFOS was 84.1 ppb with an upper 95% confidence limit of 104.0 cAwhaissladmarepnnalleroyezfseid$di9nf9gorcihnPilF2d3OreSsnt,alateegvseelsasn2d(1O2tlhseyeenDairesstrioalcldt,, op2fa0r0Ct2oi6lc)ui.pmabtiTianh.geinsTaahmseptlgueedsoymoweftegrrriecocmauelAplaencsttoerfdepPitnFocO1oSc0c9af4ol-r1i9anlf9le5ocftifotrnhosem, srpeiasgrpnteiicfctiiicpvaaennlttyls.y wa(pIsn<.3b0o71o.)t5stphripagbphe(arn9a5gl%eysoemCse1,t,rt3ihc3e3m-me3ea6a5nn).ofPFthTOehSe95rl%eavnetgloeslewrtaahsnacne6.fl7iemmtiatolef1so5:r .P0F4O0pp.Sb1.wappsMb.a$l8ae.n5dcphpi3lb5d.rw2einthphpaabdn, dueeapccprheeraasx9ee5g%grraocduounpaflifldyreotnmocea3g2le.i8mp2itpo(b2f5a.9t67.p102pbyppabtr.h.roWuIghnhesnaigmesptlr9eat(il4fi2ine.cd8arbpyrpegbargeews,hseitorhnee aginteaolwmyaessetsr,hiicgahgmeesetaw,naasntdennotdthesedingtnsoitfaririctsaeendtfl1oy0r (tPhpRe<sOeSd0al5te)aveaglsissvofeconiratetheaedclhiwmisitathtedePsFbaOymSp.gleendAseilzrtefhwooeurrgechasctihhmeigledaanrtdaearcwrTeoosrcsaettinhooetn spstruaobtvegsir,doeuhdpo,.weavegrr,aphiticiasl dipfriecsuelnttattoionmoefrplroegt aIanvnearn1ao8gt-ehmeaorgnetsthuodfpye,trhiOeoldmsaefnlreeotmdoa.nthoe(rs2I0nw0ta1esm)a5ot0ibotnyaaeilanresdIn(sstSaimDtputl1ee5s.f6o,frraotnmheg3e1Acd5av-d6aa9nv)ceeramsnedn(1tth6eomfaavlMeeresadagiencdiange1e5 (ofIfeAmtMahl)ee.sf)emoTavhleeer d5ovo2en%ro)dr.ossmweoao(srn4=5vey1heiaocrrlse3a(%cS)cDiadne1dn8.t5r,(ensa=pnirg7aetoorr1y32-3a7%4r)e).s,tTh(henaed=ctaru1 asouermsoa3f%(d)n.e=aBt4oohtrhwes1re3er%u)in,mtsbaarncadriannliitavulemrhoterims(os1ru~erhw2aeogrree(6hn0a6r=)v,e1ds6rtueogdf. awfnerdroems2ohb3itpdapoienndoerdfsrf:or7zoemdno$ntoomrs3oMcfobnltfoorroibdau:ntaelldiyvsleirsv.seamtTpislhseeusescoaonmnlspyilsaetnseddw1oefrdeo1n0oexrotrcfaotcnittsersdiubeuw.tieSndagsmeparlnuemsfoownne-lrpyea.irSfirenorgzueemnxatstraamHcptAlieoMns cepuxrrtovrcea.ecdtuiFroxnetpearnnosdcievdweuerrmee.atqrTuihaxnetsaitpvaietkrieavgeseltyufdoairsteissfaiyweededrseaumsppielngrefrHoerPcmoLevdCer-tyEoo SfeMvPaSFlMuOaSSteafnrtdhoemevhparulemucaianstieosdnervaearnwsdaussac$acn9u%ruan(ce3yxDtro2af1c7it%eh)de andthe average fortified sample recoveryofPROS from humanliverwas 78% (SD 2404). 2 ENVAMRD(2002)17FINAL wvSaaelsruue1m7m.iP7dRpnOogiSinntlLeb;veelttshweetehganet7o6mw1eet0rrenicddemtteehraemniLnfOeod.rtsTehrbueemmleePsaFsnOhSsaenwrautshmeP1l4Ri.mO7iStnoglfievnqeLul.afnoirOatFthietoh2ne43(s0LeOrPQuFm)OdwSoenrIoevresarsaisngaamnlpeylsdeesda n2an1ea0.lyazneMdd.etah1ne5 L(PO5FQ0O.%S)TlwheeevreemlsedafenotrelrmimvaielnreePdaFn1O0dSbfeleem<vaeLllOeQwd.aosnTo1hr8es.sewnesgr/aegm:psltiehmesilgwaeerormfeeortarsbioictghmneesdaenrfuovmral(luimevaelmreidP=pRoOi18n.S2t wbanestgw/1e9.L2e;n PtfheFemOarSleeposr=etr1su7tm.a2taenndgdt/hlmaiLtv)enroadnaadtsasloifcvoierartc(iamocanhlsoewf=etrhe1e9.o2b2s3negr/pvga;eirdfeedbmesatalwmeepe=lne2sm5e.(4assenurgr/ugem)d.aPnAFdltOlhSiovulegehvferltoshemadntadhteaagsweae.mreeThinnoedtiavsvihedoruawagnle). mssahemoapwnleeldsi.vaetrhgeloeomvdeelacownnaesfliav2te0ir.on8t.onsgT/ehgr.eummFeoraartnitohleiwva1ers0 tfaogeamsiaenlreu1md.o3rn1a;otirotshwewaimsthe1a.pn3a:i1.sreeOdrFusmatmhlpeelvees1l,3 wmtaahselem1ed8.oa2nnonrIgsv/emwrLi.t0hanpsdaeirrtuehmde tio was 1.321 with mean serum levelsof 169 ng/mL. and mean liver levels of 16.3 ng/g, respectively. 2 -- | i I NY eT[we Ts 5fs 3. SE -- 5ENE ae ENVAMRD(2002)17FINAL 30 Human Health Hazards 30 Metabolism and Pharmacokinetics 300 Absorption TiPnoFusOrosSlu(tiJsioowhnenls0lonag,brsoGouirpbbsseoodfntfhaornledoewOibmneagrl,eing1re9ast7st9,i8o)an.t. leTAahsftet9edi5arg%eossitfnigtvlehetorrtaaoclttadlaoncsdaercobofonntP-eF1nOt4sSi-sc1osn4ytsCatie(nmmeiedcaaonlnldyosasvebe,iroa4gre2b,edm3g4o/t5k%3g4.) OoHfofurttshh.ies td5oh%see.mleika"enTlhyesruemmperaeosfnteonftteasclaslycsaetxrecbmroienct-ai1ol4nlyiinasbf1seo.cr5esb5e%adnodcfadrtibhgeoensd-toi1sv4eeptarrteasc2et4nptlhcuoisutrhcseornatinendntths3.i2sd4i%g%esoattfiv4teh8etrhaodcuotrssei,.ssuASetosm2oe4r ainrethceondsiigsetsetnitvewittahctthceon2t4enhtosuarspaosrtesduoltsoefdaetxac.reTtihouns., from solution after administration to non-fasted ats, aTthleeadsatt9a 5f%roomftthhee4P5FhOouSr-p1o4sddoossee wgarsouapbsoofrbraetds 3.1.2 Distribution nrAoaltdi2ao4sacrt1ei0lvii4at8bylehroeaucsrotsvheearfeotdtehrwearastsiifsnsoguuleneddoairtnaaltshdieoncsceaerlcoaafrsgsPeF(vJOooSlh-un1smo4enCh,o(Gm4io.b2gseomnnaetakens)d wOiebnreerr,anse1,c9e7a9sp4sp)ar.royxTiahmnaedtehcalorymcoa$gs6esn%deaiottfaytaorheef p"sTeahrmecpeslnpetleoaeflnitsqhuefotrdsoomsweathsiend2ti4hffehiwcouhulortlateonodars4gs5aunrh.owuarsTph~oe0sr.te2d%io.ssseTorhmaetescewoxenccrreeenttairnoaantliyoozfnestdootffaotlrotcaoaltrablccoaan-rr1bb4onoi1nn414iunrrcieonnedt(enb1tl3,o%o/addnadcye)tlh.les s0ae.nl2de5cptliaavnsedtnr0ae.t3we9en.trieroencsopoefmcpctaiarvreebldoy..n-1TT4hheuisnm,eeaadtnb2rl4aotoaidonodcfe4rl8esdhboluorosdacfeelrl a0pslinagslmeaorcaolndcoensteraotfioFnC-a9t52.41a4n0d, t4h8erheou1rsnios cA1o.1Unc(Selnothrdnaastyoisno,nasfGtaiebbrosavoensiaannngedleuOgbiencrtq,auvi1ev9na7o9lbue)sn.tsdgoOstweheeorrftaiesPsFsfuOoeSlssl-ou1wcs4:hCals(iv4e.mr2,us2mc0gl./e6k,:gsp)ilnais,nmbam,oanl2ee.2m;raaktrsir,donwme,ye,aann1.d1t;sispaslnueedenlCu-hn1ag4l. d(ceo0tn2ecceutngatlbrela)etiaCon-nd1s4arbwadanogsminifgnofaurnldofmitn0.(br<2a=in1.00.O00.n86luuyggg/l)ig.v.eraTVhenerdryepllwitaalssemaCa-cd1oi4nftfwaeairnesencdfeosuinsnudCb-isnt1a4wnhtcoiolanetpeeenrytceeon(ft0as.g1ue6bcouufdtgao)nseeaonu1ds $fn9xo tedsatyess.paosntddsopsiec.en25a.re21d%ueainndp2ar.t801%blroeosdpecttlilvecloyn.taTinheedlinowthleesveelosrogfanrsadwihoeacntihvoimtoygefnoiuznedd.for kidney, lung, t"ThTheheesrleeivshetrauvdteiheabsneaerinne sdteehvseecrrsailebresudtmuidnioefdsetdcaoainmldsiu,nctsfeeedcttuisteoosnesxa3an.md4iannneedotnh3a.et5.el,evIeanlnsgdeonfeprlPaaFlc,eOnStthaelinltetrvhaeenlssdfaeormf,oPffFeOPStROaaSnrdhhnaiesgohnebareteei,nn demonstrated 313 Elimination CiUnr1ti4rnaavraeydnmoeiuxnscirdsetotesireoendofi(sPJtFohhOenSs-porn1i,4mCaGri(yb4.2sroonum,tge/aoknfgd)eOlibniemrmi,anla1te9i7or9ntbs),.fomr ePMaeFnaOunSricniunamruytlheeaxtcirarvte.etifoBencyawla5se9xc3dr0ae.yt2si+o-an1f.wt5ra%sao1fs2it.no6ga1l!-e 12%. Radioactive content infeceswistoalow tomeasure aft6r4 days. "The half for elimination of total POS carbon-14 from plasma aftar single oral dose (4.2 mg/kg) in n ENV/MRDQOODI FINAL pmlaalsemraatssamispl7e.5s dfaryosm (gorhonusposon,f Gthirbeseoantanadt O1,be2r,,6,11927,92)4.,45T,hi9s6,deatnedrm1i4n4athioounrwsaafstebtasheedsuipnoglneaonraallydsiosseof dTmoheseaerneeplieisrmceievnniattdaeegdnecoveifaofPfeecneFtsberyOohceopSdnatotris.ocelec1liaritcmsu4il(na8at.Cti0oend+-ovfi0a.P3f,OeJcSoe.shn(s7Ao5fn.t,8e+Gr-i2b51s.o0d)nawyasansdof9O.bc5eh-rfoo,lleds19tt8yh4re)a.mmeinaAefnetpsreeraacdtemjneutnsattg,meetnohtfe acfoomnroturtnohletsaramtoso.funcAtafriobefornc-Pa1Fr4OboSrn-e-1m1a44iCneixandcgmrienttioesdtbrieanteiuxorcnir,neetth(ee1d8m9ae%raefno1rl9icv%oenrtfrcooarlrscbhoaonnl-de1s4$tcy%orfnaotmerinnctch-oartlee2as1tteyddraayrmsaitsrneeap-rnredesaet7net4sd%,1t1fho%er caaeunltdlho4cr0asr%cbooonnfc-tl1hu4ededcootnshcetenfttohrreacthhiiooglnhessctaoyrnrecaemsniitngrena-ittfirioecnaaonttefldyPaFnlOdoSwce-or1nt4arCfoelirnat2ls1i,vrdeeaasrtypes2cttooifve3lcyhw.oeleeMksestayarnfatmpeirandesosatirneagantadmnedrnetdt,hbelTfoahocedt tihatccohnosliedsetryarbaimeiennetterreoahtempeanitcecnihracnulcaetsiofneocfalPlFiOmSi-n1a4tCi.onofcarbon-14 by nearly 10-fold suggest hat there 3.1.4 Haltife in Humans cIflnoilmolirondwaeetrdioinfoorhdaeftlieEvreHmEiann(ed12t)aheohfahall1ff4-e2lia8fredsaoyf(s3PM(RaOpCSpo,rmop3xainrmeyat,itree2el40sy00wy6eh)a.ors)w. oSreAkreudhmailPniFt3OeMSsstulCedvhyeelomsincsaualggleDasrigtveeirdsigoarnoumwpeearonef erpteeatrlii.roede.s20(0Tn2w)=.o 2i7Tn)htieerifmcrusrrteepinontrttlesyridumensdrceerpriowbriatnygs,utgihngeewsrhetsieudclhtas stseherurusummfasrhaahmlapfvl.ieefsebaeorefen d1sr3u9ab-wm6ni4t0eevddeary(ysB.u6rrmHioosnwetethvaeso,r,v2e0tr0h03e;reSB-wuyeneraser spleeevvreeilrosadlmoleniamsdiuitfrafetediro:en2nst)tsdoaetyrhsuesmuesiwanangaslsyalsnieagshltylizynecddliudafiffnreger:neta1ac)nhatlchyoetlillcieamclittiteoednchdpnaeitrqaiuoeadsv:awiialtanhbdloe3n)alyntdhontrheeefmereraaennsgcueeorefmmaestenertriuapmlerpPuFtrOiiSmt.ey eowrxaipsgeirnniaomlte2ndt7eatsleubrejmemicontrse,.d iuSnncetilrluudamifnsgearmspythsleteesfmrwastetirce3acsnoaldmlperclateensddhofamrdoembrrecoearnchianonaftlthyehzeeadsn.ualbyjtAeicnctasleofmfvoerett4hrowdta,ismiemnapveodrleivoitdnosgms9ipnaoinfmniitsnheeg "s1Ty5hs0itsedmawayost.uiclmdeerarasolurlrioenwdhefironernitpstlianitcitsahtteiecaaswlsiateyhvaeallquluaattliilmoyenapfooffiencttthseedfrepoarmeccheiassciaohmnpsluoebfjuetschetedafnmoaelahyszauelrdfefmineentdhteetsaernamdmienaaastnsiauolrnye,ticthaaltruanl.l O"aTnFhdethatehvee9raaevgeierraaeggeeesonfiuntmchlbuedrreoedtfirinemeosthnwitsahsasn6ar1leytsyiiersae,rdst,whatrhsee wm18ee9ra.en 7nAuvmmeabrlaeegsreoafnBdyMeI2arosffewmtoahlrieksse,gdralaoltupfDerwcoaamtsutrh2e7w.9aD.esc2aT7th.u7er ypmelaearanst,n PROS value at study initiation was0.89 ppm (range 0.1 3.53 ppm, SD ~ 1.07), nmTuuhlmetbimevaerraionafblsyeeerarrusemgrwheoaslrsfki-eolindfeoarnfaolyryesaPerFss.OsSinTwcheaissre$ta6inra7elmyyeseniatsrswhea(rsreanangtoette2ms.pi2tg9eaidfitc2oa1n.tr3epdyrueecadreisc,teoxSrpsDeroif=mes6ne.t1r2au)l.m chraArlgofer,ivineBsMIth,ien dcperotonedtruimcnituoneadftoniotonnh-oeorfcccuaopamhrpailoofun-alnlidf,es lffooowru-nlPdeFvOienlS.tehxepHorsoeuwtriereveoesnr",btlhsoeeovdhe;arlatl-hieifrseesfuoiesrseu,nskPhnoFouOwlnSd. biseSleinckooetlneydd.,bePiFFnigOrSsp,riostdhauecmeeedftfaibenotltihocef bfHloouwdoeyrvowechrhi,elmeiitctmhaelasystiunndoytthwbeaesbaopodpnyrg.oopirngiF.aotuerTtthhoi,rdtidhrieisct tilesysntcoitommaktpneaoriwesntmhiuectrhheersheuilagsrheeorfinttthheearnaacnttihimaoatnlrbheeaptlowFrJetieefdne PidnFatOlaaSbwaiantnhdimotahtlehsse.er amettahdoudectooulpdotbentaeisahlilyghdifafser+/e-nt2p0ro%teainndsbtiinldlisnagtissitesth anddaatfafiqniutaielsy. cFriifttehri,a.systematic erorofthe analytical 2 ENVMRD(2002)17FINAL 32 Acute Toxicity oFfouarts.repaonrdtsoonfe adceutremalstaudnideseoyfe iPiFtOaStiohnavsetubdeyoenfsraubbbmitist,ed, one inhalation toxicity ofras, wo oral studies WiInrnaiaighstttuoddyuSstp-orfadegeetudeerm-meDicanhwealtenhyiesrmmteswd,iitSahinsdlcrexytghaarilorucapot,nacelfenlvtoerwlastrioaotfne o(189LfC.1o12)0, R41u56s.9cl7hitemertgslaplle.r(P1mR9iO7n9uS)tetaodwmaeiisngihssteteedrtetdogaPrdoFmuOipsnS.isdtuesArt "2tT.h8he6e.PcF4oO.nS8t8rd,oul6st4g.9ro,uR7pa.t0r5sa,twse13wr.ee9i,ge2h4x.0ep9 das2co0dr3-4fo25r63.| 9ghgo7ua.rt.sTtTuhdheyceointnetistrtioagltiroaont.uspwrTaetrhseeweteeistgxhgerpdou(2op00d1rsr-ays29eaw9ied8raetatesxftpluoodswyedaitne(i0toiaf1t.i8o19n5, eaBxibtpneoossrumpraeerl cmshiiangumntbsee.prr,ioAhrloltuorolteyhxepfroosrpurfrooetu,orcahotlosu1r5ws-emarifenteurttheeexipsnotasemurevraela,ssadtnuhdreidntaegislttyhgetrho1eu-rpheoaruafrse.exfpoTorhs1ue4rerd,aatysast.sreerImenodvioavbilsdeufrarvloemdbotfdhoyer srwaeepmiopgrhltteessdwwteehrraee acrlolelclaocnrtidemedadlosonndDyDaianyy1g40spfo(rpnortmiaonraello(0ussuelrxyvpiowvsieunrrgee)a,nneiDcmaraloysp,s1i,beudDtaaaysnas2l,yosoDensaaywse4rp,eonsDsoaiyblper7,ovaaifdneedrd.dDeaaTythh.e19.LBClsIotoo1df W5i2lcmog/xlon.and 95% confidence imitsof4.4 and6.4 mewerecalculated using the methodofLitchfield and ah"lTashoepthoieigrnhmtei,sntoatdleodcsyaearnglirymoau(lpDs,asy4u5r.1)v9i7bvemecgda/ulas,nedwoabsfalonomodetscahumasnpeildceaisnlwtephrreoeLbltCeaskmedcnaulartciuntlgeartmeiixonpnaostsiuaornne..d tTTehhreemsi1e3.n9aemndiymoa)nlsDgwareyorut2p. awlaAssto not used in the LC determination amnloltrthtaheleist2ey.4g.r0oA9utpms4g8/sl8h,oewx2.pe8od6s,suiragenndsgro1of.tu8op9x,imcgali/ltlyatnihinemrcaelluwsdaidnsige2ed0m%ab,cyi1aD0ta%oy,n,6a.rnedd 0Amta%te7mr.oi0ra5tlaalainrtdoyu,6n.rde4s9tpheemcgtni/ovlesletyh.oerreoTthwheaersrant5ass0a1%l egdeixnspecorhsaaulrrgeep,ogroyoreuplcslo.onwditmiaotne.rialAbanroorumnadlthienanfogeonbistearlvarteigoinosn,wedrrye rraelpeosrtoerdottohebrebrleesasthfirnegqudeinsttuirnbatnhcees,Toawnedr sHAiCugneniefwcicraaosnptsaytl,rseotahtoembesmneotrsvteeldcatoienmdmcaoobnnutiraooblmnarolartimsyalawinatdsy twvhaaerrsyeifdnoigrsecdoemlgoarryeaetisnooontfdobifestcthoreleoafritavmteeirnotannordeftlalhtueendg..fivTDerih.secroeAlfmoorroaent,igotnhaenoifmmoatslhtse tthhaetsdmaildlpirnetmeasttuinreelwye,redeaclrseoaosbesderbvoedd.y weight, discoloration ofthe lung,anddiscoloration snd distention of a0I.ca1e0t0so.tnued2'y185f0o,%4dc6e4ot,emramoniidlne1mi0tx0hte0uramecg.u/tkeAgolrlPalFdoOLsSDe.blyevDgeealvasangweee.trael.Tahd(em1i9pn7oi8sw)tdaeedrrmeeiddnaitssetsvetormeladutmeCreiDsalorftwsa.1s0Ss/mussekpgernodbueopdd,yidnow8esie2gs0hot%f `r"eTexhcpeoorsrduaetrsde,waaetniDdgahdyeadil7y1a7tn2hde-r2e1Da2aftygera1f4t.orthT1eh4ebdeagryaist.nsniwInetgrosefroetbphsoeretrsevtdeuddtyhfaoirmalmalebadnnioirsmmeaalllys dspyirgiinonsrgtdsouprodinontsgainntcghoeausnfldoyuwrweeihrgoehutrgssrowasefsrelery necropsid, as well a all rats that survived to the end ofthe 14 day study. Afcemuatleesor(a2l71L(D200-v3a6l9ue)smaenkd )95a%ndccoonmfbiidennecdemlailmeitasndwefreemaclaelrcautsla(t2ed51fo(r19m9a.l3e1s8)(m23a3ke()1.60-339) mg/kg), mAlel aks ginrotuhpe 4d6i4edandpre1m0a0t0urmegly/.kg doItseagprpoeuaprss dsiidgnsbefoofrettohxeicnitdyomfotshte sftruedyq.uenTthlryeeabasncirmvaelds iinntchleud2e1d5: 2 ENVAMRDRO02)I FINAL hwoygpeonsictiavliye,giodne,crsetaosmedachlidmibstetnotnieo,n aanndd saitganxsiao.f iiAatinoencorofpshye, golbasnedruvlaatriomnuscoisnac,luadnedd:lungyceolnlgowe-sstiiaoinned 3IWn0is0atgasrreacanolbn.idn1oot rawaiasss(td5uedt/yes.erxGm/aigbnrreoidueptl)he(t1aw9co7u5td;eoscoeriatsloedfL iDnPiFgDOewSaansbygertgeaaaLtv,earg1te9h7ai8nn)5waa0tdemmrig.n/iksTgtheearnerddatlsteswwsoetighgrahnoeud1p5sb0eo0tfwmSegeh/nkegr2m00andrAaeltlasdowftoenrheeDoarbyasse9.ravdeNmdiofnnoiersstoieftrgehndseo1fr5a0tt0soxamidcgmi/itnkyigasntdediermdeodbret5af0loirmtegy/tbkhegu 1diti-eiddsanbyoetfoobcrsleeeratrvhaehtoinowdn ofprfeertqiuhoeedntesltnyuddeytd.h,eywIiwteihrsetshaoetbeledaerstvrheatdt da2en-ad3thnhoooucircnsudriarvfeitdde.uradloNasnoiinmgianladniddvait"dausaewlvaesrpealptryhoovdlieodpgeryde.sdsaeItdtasoswrteasretemeidp-rctoovhimadaettdho.esehTMiTgbhhyed2oa4suethhgoourrrosus:ptasrttaeatsysinwge"rGierno"tsdhsiespprsaeatctheeoelduon"gtiiaclt examination revealed nothing remarkable." BapbriboeicsteesdmuehriaeedrstatahntideeHsaheraiirtlihsierp(0p1.9e57dg4)foeorxma0m.ti5hnemeilrdbtaahncedkpstohaeenntdtieafsltlanmfkaosrt,ePraiFnaOdlSiatstaopscpuaepuapsrliesesfdikvibenytae3nndtMhesywoeafosranatisogolrnia.d)m o(S0fi.x5thagsl;btithnseo hamocatucerorsridaailnngdwa17s02 phaloaucsretsdndotahnerdacibozrveaeddreidnscgoaslfe.wnetraecNt ropermeropevafereedrdenactneedst tisshietepsdr,eotgvhrieedneeodcfoevfroeryretmdheewtmihtaohdagnaodurgeedscepoamrtacihnegwsa.sofrAerfceosrurldt2es4d Cskoinnceinmtirtaattiioonnsocrorteostwalerdeose0o;fwhteiscthmiantdeirciaatleswnaosrmoteprodviodderd.sweeTlnlnianlglidsextnercatgbebdi.s, it is reported the primary raInunbdthtTehHee1ry7eeywiemsai.tsatIitsooanplspdteuaadsrys,suospnipxeliateledbnbtihynoof3NaVeD)wgroZafetmahl(ea0.nt1edstgW;hsuithbtesetparrnaocbcbeeitdwsuarwseeirsnetsattpiellslaecedetidhneironn0ce.o1lvlear,sortsh0oe.1toht1ehayercwoeauysleduwneaodst rbleeufpltobrautrnetadrentadhtaeptdatlahpseeabrreacalocntctiorononlj.ouncTtthhieevtaeccsotnatcmeant1,terr2ia4at,lio4wn5a,osranrtedoatda7l2adghaooisunesrtsofaftsthceearlteteorsetfatsdmueabnmstta.agnecTeohewteshrceeaclneoomteenpstrorvraidsewdea.rnedintohites pmwareteresereinmatalexdwiamosarlfrleauftesrh|eenhdcoefudrr.oamnEdathc2eh4etyheio.muersTthhaeefreecryietssrnewaotemrreeenftesrcteohnrecened,dperaconrveyiadaseceddcufoomvrueltrahetthemedertdehitosodc.fhatrhIgteeaspotpruedaryress(iBtdiuheetsoosmfceottreeeurs and Hari, 1974), 33 Mutagenicity SPDaF3lOmsoStnwewilanlsaoe~sftEeSsdacchfceohrraitcrshoiambayiccloietlsyi1cM0earimenmvdiauslciieaaemnu-(tLmaititictootnnosinBoimtoheneerAtecmvsee,rsseISncam.lu,tmao1tn9i7eo9ln).laMsiscaItryow(saMosemcecshsPilo,atet1e9Ts9et9es)dt;a(inn2:d) na(1)thiaen vaniestgsraaotyiavfseosraiynunaflsolcrhaecsdshaurylosemdoinsDowNmhaiAlchsaybintetrwhraeasstiistoens(steUdiD.nSh)PuFimOnaSnradtwohclsoivlenerotbplironiodmduacrelyyrcmeepvlheorsccueyltmteuusrteas(tMi(ouCnri,faonte1h,9e991h)9i;9s9tia)d.nidne1(l3o)wcauassn. OdacFotSci.svatntoyitopnhiianmndudurcideuomcehsorrnooamttotishnoedmutacrleypaUrboDeprShraanitniloponrcsuismianorhyfEu.cmualcntoluirleywsmhopefhnorcattyetshteeespdatwwoihcteyhtneostr.eswtPietFdhOowSuittwhmaesotraabwlosilotihcteoaswctteimdveaittnaiobanon.liiicn (iCorCmDo-u1sBeRmimcircoenuacnldeuiss naesgsaatyiv(eMiunlith,e 1m9o9u6)s.e bPoFnOeSmadrirdonwotmiicnrdouncteclmeiucsroansuscayl.ei in the bone maou of Tpe2r2fl4u7orooCctoaCnesuwlhfioncahte iisn waater0w%as bteystedwefiogrhtits asboilliityonto oinfductehegendeiemtuhtaantoiloanmminonSiusmtrainsasltof of 2 ENVAMRD(2002)17FINAL w"pTihtehhocuhitemmimectaalbaowlniadcsaalncsetogivafattoiivoiens.inaTb-ail2li2t4y 7tsotCriaoinCndsuowcfaesSraetlcemsootmenbdeilnwalitatihaoSnn.ditniynSp.h5.icmeCureerrvieiuvsmiisasierasseitnaDsi3nTwDA3hS.eSn(aStniedsmtmTeodAn1w,i0t01h9f7an8n)da cdeosnidcictaitoonrs assay for the detection of volatile compounds. It was nonmtagenic when tested under these 34 Repeated Dose Toxicity ao`Tnvhereedebie9et0ea-nrdycaoysntdsuuudcbytcehidnr.oantiBs.cotshtIunrdeiapedosdroittfsiowPneF,rOaeSufnohauaruvdewietbeeedkendarcnaodfnasduac2tt6tehdwe,etetikwmeoocagfpasvtuhaligeserssettvuuiddeyiwe.isninn rhoesums maonkmeoynskeaynsd 0fI.ne3mt0ah.leesr1a0tw0es.iugb3h0c0he,rdo1n1i06c005sto-urd2yg3,0a06G0to0lsdpteupndmtyhaPilnFitOeitaStai.o(nF.(C1T-99h758e)bd)fioartdam9i0ynidlsaetyvsee.rlesdwTCehreIemreaaqtluse,isvSa/wlseeeinxtg/hgoeroddupo1,s9e6ds-io2t3af2r0y-2l.aevn6ed,lst1oh5e,f `c6co0onnasdnuudmcpt2etd0ia0onmwtgh/eekrgeb/edrgaeiycn.onridnTeghdeowferetakthsleyws.eturdeHyeomabanstdeorlavofegtdeircda3ul0lyaannfddorc9l0giennidceaarylaslcohcfelimtnirisecatatrlmyesnaitgn.nasl;yTsbheosedyarantwdseiwugreihrnteaslysasanicsrdifwfieocroeedd asaffpttleeerren9f.i0xpaidttauiiyotnsa.roy.fTikrsicsdaunteemsyesn,wtetraeesntdepsraleosvgearrroviscessdnaienncdrbobusrfpafyienrwewadesrneecuotwnredaiulcgthe1edd0..% TfAhotretmnhaelycirrnoopistdhypeatrheaeytehhsyeraworei,rdeliwveperrr,eesaewdretvieengddhlesfd,n dmRuuiscosrdeoelsnlc'ouspmi,cfaiilxlaltyei:vue,.adjreejnuTalnhsue,m,afoocrletlcao,uwmi,bnrgacionlo,orngc,asnorspehcatfgurumos,m,kcieodynnetesry,osl,gallailnvbedlr,adadllelurn,gt,rheesaaktrietnd, (mgwerisoteuhnptsceorriwocenreal.ryymepxvheasmnsieonlde)e,d. tbrhoeyntrreooildpb.hoanrpeayrnamgteaharylrrooliwdy,m(pttihjhuymnnucosdt,ei,otnrma)ca,hmeams,aaltriovynasriygllgatlnoadnn,gdu,en,elruuvrmeibna(arwriytsbphilnamaduldseccrloerad)n,,d psaipgtluieientnag,ry,pasntcormeaasc,h.persotneseovaernicse., bA1le8lt:2wo8efetnfhoerdartyahste 8im-na1l4teh,esa3na0dn0d,at1f3e00m0a00l0easpn,pdmr3,e0st0pe0hctpirpavtemslydg,ireoidunpbstehtediwee3de.0n0 Ddpeapaytmsh7g-or8cooucfpu.rteredAattbmeetn1wt0.e0e0nTpdhpaemy,sratd1se3a-itn2h5alaoncgdcruodraurypessd. raserhdoouuwcneeddd tsahicegtinevsoyieftsyt,aoxnyideclmilotoiywstimnarcteledurdimiaanltgeareirmaoalcuinaadrtoituohnned,atcnhooengvmeunliostiaolunosrrtenfgooilhoslneo,wiinncgrheaansdeldinsge,nshituinvcithyedtobaecxkt,remeadl msaitemruila,l taTenhrdrmeitenhaemtaitolwneo,s fmaenemdaanltewsbooddfiyeemdawdleueirsgihnitngstwhweeeerk1e0$0repadnpudmcetwdhreobtuyhpir1dd6ime.da7l%periadonirdedto1d6su.crh3ie%ndguilwneedtehkseac1rm1iafliocefe.tahnedTswtfouedmoya.fletAhetgrmsoatulupdesys. crreeosdmpupeccaetridavbellfyoe.rammaAlovenesratahgneedcoffnoetomrdaollceasonndsatum10p10t0i0popnpmpdmgu.rroiunpAgsf.ttehreCli3enn0iicrdaelaycsshuedomyfiswtpcreayrtiamonedanlt(y,gs/ehasetmadtaatoyon)lcowgmaioscntvshailgsuehisocwawenedtrye3 PcsriPeg.antihfiineciannettwpihonocssruperhavosikeviinniangsmemaelaaennsdhagalldkuasclioigsnneeifpiinhcoamsnaptlhleyastr,aesdbeulcoveoaddlueeursryetfahorroncfiyettmreao,lgeehsn.emvoaAglfluoeebrsi9ni0,n hdmeaamylasetsoocftrarintedaatfnmedmeanllteeuska,oca1yn0td0e saclosiugnshtltsi:gthottlmhyeodlteohrwraeetreseuirrnyvctirhveriaonscegystfeie,nmaphlelemasastmhoaacdrgilstuitgaannmidfiicclaoenxutaklloyaccryeettdeiucccoaeudnndths.peymroTugwvlioocboitnrfaantsnhademirsenutarisvceiuvlaoictcniygvtietfiecemso.aulnetUssr,isnahaslowyweselidls tsrihegsenuilftfesimcawlaenestr.ley ciRonecmlrapetaairsvaeebdklieindantemhyeonwmegailgtehrstastaenwdderaaenbsdsoilcguontnitefriacoalnndgtlrryoeluiapntscirvaeetals3ve0edriannwdebio9gth0htsdseaxwyeses.r.eRseglaaitifvceanJtilveyrienicrgehatsedwaisn 7 ENVAM/RD(002)1 FINAL wAelrearsediunctehdeb3y08p.p7mangdro8u%p isnurtvhievmeadluenstialntdhefeemnadloefs,trheespsetcutdiyv.elyA.t sAtvuedryatgeermfionoadticoonn,smuemaptnibonoddyurwienigghtthse ewnetrierecsotmupdayrapbelreioadm(ogn/grauthdeayc)onwtaroslsagnndif3i0capnplmy grreoduupcead f3o0ratnhed m9a0ldeasysa.t 3O0neppmfe.malHeemsahtoowleodgicslviaglhutelsy e9l0evadatyesd.gluocnoesemlaelveel asnhdowoende mmaoldeersahtoeweidncarselaisgehstlyinincgrleuacsoesde,alkballoionde phuroesaphantiatsreogleonveaand30y-dgalyust,amAylt mtraalnesspeaptdidassiegnaicftiicviatnyt. deTchreeafseemsaliensahbasdolsuitgeniafnicdanrteliantcirveeasaedsreinnaalbswoeliugthetsa,ndabrseolaltuitveethlyirvoeridwepiagrhtast,hyrToihde wunecilgehatrasnidncaebssiomliultaerpcihtaunitgaersy wweeirgehtn.otTnhoteedbiionlohgiigchaelrsdiogsniefgircoaunpcse.ofthe changes in male organ weights is Adteneescroofpsdyi,sctorleoartamteinotn raenlda/toerdegnrloasrsgelmeesnitonsofwtehreelipvreerssenntd diinscaollortartaitoendogfrtohuepsglaannddulairncmluudceodsavaorfyitnheg csyttomoapclha.smicHihstyopleorgtircopehxyamoifnahteipoatnocayltsoesshanodwefdocallesinoencsroisnisalwlatsreoabtesdergvreodupisn. thCeelnitvielr,obtuhlearin1c0idmeindczeoannadl preplamtigvreosuepvse,rittryeawtemreentgrreealtaetredinhitshteolmoagliecs.lesIinonasddwietiroen,noetspeedciianlltyheamproinmgarryat(stihnymtuhes,30b0o,ne10m0a0rraonwd)30a0n0d stehcyomnudsa,ryhi(sspcleoenns,ismteesdenotferdiecplyemtipohn ninodtehse) nluymmpbheoridanodrgasnizse, sotfomthaechl,yimnpthesotiidnesf,omluisccelseaanndd sinkint.heIbnotnhee smiazreraonwd hnypuomceblleoufrlaliytympwhaosidnofteodl.ileTshendsplceeelnlswaans daslisgihmtillyaartdreopplheiteidonwiwtahsacnoortreedspinonthdeinmgesdeenctreeraisceliynmtphhe nhoedmeosr.rhagMeuscowsearle nhoytpeedrkienrtahteosgilsanadnudllaorrpoarctainotnhoosfitshewsatsomaocbhs.erDveecdreiansetshein ftoherehsitgohmtacahndantdh.icmkuncesossaolf hthyepeirklelrawteorseisnaontdeldoirnactahnetshmoaslilsiwntaesstninoet.edAintrthoepsykionf,the skeletal muscle was noted, as well as epidermal oInf0t.he10fi,rs3t0r,he1s0u0somro3n0k0eymsgt/uddy,aGyolPdFenOtSha(lFeCt-a9,5)(1in97d9i)staildlmeidniwsatteerrebdyrhgeasvuagsem.onTkheyesm,a2l/essexw/egirgohuep,d d3o.s05e-s 3ge.n8e0rakle catlisnitcuadly siingintsiatainond abnoddythewefiegmhatlseswewreeigrheecdor2d.e7d5-4w.e1e0klkyg.. The monkeys were Hematological and observed daily for clinical chemistry adnaaylsysdeusea1n0dtuhreindaelaytshiswoftehree cmoonndkuecytse.d aAtUthneecbreogpisnynitnhgeohfeathre, sltiuvdery,. adTeheenaslts,udsyplweaens, tpeirtmuiintaartye,dkaifdtnerey2s0, tepsrteesselrovveadriiensabunfdfebrreadinnweuetrrael w1ei0g%hefdo.rmTalhien;ththyeroeiydepsarwaetrheyrporiedsewrevreed win Reussielalfg'tserhffiixxaeattivideo.n. TThiessfuoelslwoewirneg ocrsgoapnhsagufsr,omeyecso,ntgraolllbalnadddear,l hweaeratte(dwigtrhocuoprsonwaerrye veesxsaelmsi)n.edduomdiecrnousmc,opilcaeluly,: jeajdurennuaml,s,ceacorutma,, brain, colon, nveecrtvuen,(wKiitdhnemyuss,cllei)v,er,Spulnege.n,spkiann,crmeeasse,ntperrositcatleylmutpehruns,odbeo,nret/rboopnhearmyanrgreoalw l(ryimbpjhunnctoido.n),masmalmiavarryy ggllaanndd,. uurmibnaarry sbpliandadlercoarndd, vpiatguiintaa.ry, stomach, testes/ovaries, thyroid, parathyroid, thymas, raches, tonsil, tongue, AmIoLnkofetyhseintrtehaete1d00momngk/ekyg/sddaiyedg.roTuphedimeodnbkeetywseeinndtahyes33-050, mtgh/ekmgo/dnakyeygsroiunpthdeie3d0 bmegtkwgeiednadyaygsro2u-p4d,itehde "bTehtewemeonnkdeayyss f7-r1o0m, aalnldthtehegrmoounpkseysshoiwnetdhesi1m0ilmargksgig/ndsaoyfgtrooxuipcitdyieidncbleutdwinegendedcaryesas1e1d-2a0ctoivfittyr,eaetmmeensti.s wpriotshtatsioomn.e dAitarnheecar,opsbyo,dysevsetriaflfenoifngt.hegmenoenrkaelysboidnythtere1m0b0lianngd, 3t0w0itcmhgi/nkgg,/dwaeyakgnreosusp,s choandvulyseiolnlsowiasnhd ebrmoowrnshdaigsecolaonrdatiipon odfeptlheetiolniovef;thheisatdolroegniacl ceoxratmeixnawtaisonnotsehdowiedallnrocamtiecdrogsrocuoppsi.cNloesoiotnhs.er lCeosnigoensstwioenr,e noted. x ENVIMRD(2002)1 7FINAL I1n5thoer s4e.5comngd/sktgu/ddya,yGoPlOdeSnth(aFlC-e9t5a)l. i(n1d9i7s8t4il)leaddmwiantiesrtebryedgarvhaegsuesfmoron9k0eydsa,ys2./seTxh/egrmoaulpe,sdowseeisgohfed0,2.05.55., g3e.n5e5raklg caltinsitcuadlysiingintsiaatinodn abnoddythweefiegmhatlseswewreeigrheecdord2e.d73w.e7e5klkye., THheemamtoolnokgeiycsalwearned ocblisneircvaeldcdhaeimliystfroyr a"Tnhaelymsoenskaenydsurwienraelyssaicsrwifeirceedacofntdru9ct0eddaaytsothfe brezgaitnmneinntg oafntdhaegsrtousdsynaencdroasftpeyrw3a0sacnodnd9u0ctded. aoAftryneeactmrseonpts.y tthheyrobiedanpa,raltihvyerr,oidadwreenraelsw,eisgphleedena,fepritfuiixtaatriyo,n. kiTdinsesyuse,s wteersteespiroevsaerrievsedainndbubfrfaeirnedwneeruteealwe1ig0h%edf.ormalTihne: wtheereeyeexsamwienreedprmiecsreorsvceodpiicnalRluys:seadlrsenfaixla,tiaveo.rta,Thbreanf,olelsoowpihnaggoursg,aenessf,rogmallcbolnatdrdoelr,anhdeaaltl (twriatthecdogrroonuaprsy. voedsese.lsr)e.trdoupohdaerynnugme,alilleyummp,hjoejdun,umm,amcemcaurmy, gcloalnodn,, nreercvteum(,wiktihdnmeuyssc,lei)vrs,pleleunn,g,pasnicnr,easm,espernotsteartiec lymph crus, bthoynrco/ibd,onpearamtahryrrooiwd,(tihbyjmuunsc,titorna)ch,ess,altiovnasriyl,gtloanngdu,e,luurmibnaarrybspliandadlecroardn,d pviatguiintaar.y, stomach, tesesiovarics, sAtluldym.onBkeegyisnniinngtheon4.t5hemfgir/stodrasyecgornodupdadyioefdotrhweesrtuedys,actrhiefsiecemdoinnkeexyrsemexihsibbittseedeseingnwseoefgkasStraonidnt7esotfintahle atrcatcitvittoyxaincidtyjuisntclpurdioirngtoandoeraetxhias,heomweesdis,mabrlkaecdk tsotosoelvearnedrdiegihdyidtrya,ticoonn.vulAslilonosf, tgheenemroalnikzeeydsbhoaddy dtercermebalsiendg awnedekpr5o.siaAtfotne.r 3T0hedamyesaonfbtordeaytmweenitg,htthdeerecrweaasseda fsriognmif3i.c4a4ntkgreadtuctthieonbeigninsneirnugmoftchhoelesstturdoyl 1a0.n2.d7 a k5g0a%t aremdouncttiohneicnonsterroulmanadlktarleianteedphmoosnpkheaytsa.seHiacsttiovlitoyg.ic eAxtamniencartoiposny,shmoevaend osrevgearnalwteriegahtmsenwtereleatceodmplaersiaobnsl.e hAlald tmhoedemraalteeadnidfffuesemaaltersophhaydofmathrekepdandcirfefautsiecelxipoicdridneeplceetlilosn wiinththdeeacdrreeansalesd. ceOlnseizemaalned alnosds towfozyfemmoagleens gcrhaanrualcetse.rizeTdwboy demcarleeasseadncdelosniezefaenmdalleoshoafdcymtoodpelraastmeicdgirfafnuusleesa.trophy of the serous alveolar cells SAtluldym.ortihkeemyosniinketyhs h1.a5dmdekcrdeaaseyd agcrtiovuipty.surSviigvnesdoufntgialsttrhoeinetnedstoinfalthterascttudtyo.xicDiatyriwnegrethneoftresdtowcecaeskioonfaltlhye sutruidyn.gantohreesxtiuad,ydaenhdydirnactliuodneodrbgleacnkersatloolb,oddiyarttrheemab,lmiungcowuesreinnottheed.stooAlltahnodubglhosotdaytissttiocaollsaitgntihfeiecnadncoewftahse 2o.t93ackhiiaetvetdh,e ethnedomfetahnebsotdudyy,weaingdhtthoefmtehaenmbaloedsydwreoipghptedoftfrhoemf3e.m1a5leksgdartopthpeedfbreogminn3i.n2g2ofkgth1e0 s2t.u7d3ykg(.0 ONenceroopfstyhrefveemaalleedsnhoatdrevaetrmyenltorwelsaeterdumlescihoonlse.sterol and another had 4 reduction in inorganic phosphate. tAolxlicmiotnykweeyrse innottheed0.o5ccamsgi/oknga/lldyaydugrrionugptshuersvtiuvdeyd uanntdilitnhceluedneddofditahrerhsetau,dys.ofStiogonlsso,fagnaostrreoxiinateasntdineamlestirasc.t Oreclcaatseidonlaesliloyn,s.decreased activity was noted in three of the monkeys. Necropsy revealed no treatment SPeRrOuSm lseavemlpsl;eslifverromsammopnlkeesyfsroinm tthhe0G,,00..55,an1d.51.a5ndmg4/.k5g/mdga/ykgd/odsaeygdroosuep gwreoruepsinwdievrieduaalsloy aannaallyyzzeedd ffoorr cPoFntOrSollgevreolusp.s, Edxicfefpetrnftorantihmeaclosntwreorleaunsiemdalfsorwsheerruemaannidmailv7r3d5eStMermwiansatuiosncsd.iPnFbOoSthltehveelsseirnusmeraunmd wleivreer developed by pyrolysis; precision iesmatedtobe = 10- 25%, ENVAMRDQOFI)NAIL Loslsof PEOS n serum fp s rTr e T TT) I 7 -- TE---- [[sHoard TTiiJ T ssmmppggaenn a a o ] ] No esxpelpanaartidoanyss nod geavefothethseameelaetisvlel.y high serum eves in the controls. Sample 355M was run on Lodo EOS nther [VeooMr t T[Doosage Level T[PaROwSinLivermg) pyPROSTo Lieverepney)| [r es Tosmpe igty DT ow w p o] [C8AsMr Tlais mb TTaiT oo oTew o ]| 1LV0iivueiTrdsurwoermewtasshtosrcaesmdipelmeastg.edeAvttihleflno:resevRoefearcanolavmelorynyt,hosofpliPyFrpOaSraanlTalosymasmispt.lheeThwisveerrr ksseadmnpliienshtihwecashsepheawraitamiaottneodoffsshooomdme pTVaheleruieusmrewinentresehwviahglehueerchaaklnnioeuwxpaneecastdmseodP.uFntBOeSocfawuaPsseOcoSafieivaa,sledate0.d1313110p4ImcvoenintoshlepTplev.rwaHs eavl3yszedshfersruonl,rcionnteol TPHhiRogOhreSrmaailyntuhbsee iibnveecsraeurasutemthaaneny1.l5aivmSeaagaesypfepmraoJlievms,autrlvSyievterdenusenamlvelsachwteehdreuel0e.n5dogsdhertaeoiynIenldetvhaeetlt4bh.ue5t4ar5sksmugbdshataynteidaovllty erous. eFrom his sud, therdo mt apps be any dlrmnes in PFO in her scourfmo baweon Teuhmmebpeevrar,loureossneofoflPeElOoSnandpinratohndeslfeiomvfaeltea,cndofaseasrniunmmaoslhsdoepulel.rd gbreAouUvpp.ioeuwgeShdtorwPaiRgteOhSocaitstisoena.bilveeTrhtharemepTlwaeeerseoa3tclovfefrcgyielaniieeodn cundeeriwenntaScotmesdiencothravtiisoaansivmadtieonnocbfsyeotlhdedSHeipdaiPoohfanse qmuitd phParseec,iseSarmpelmeesreomnay lo hve cIrnoneodnudcaetdrmeidtnoisdwteeereeemdkidnoeasnestgh.eoffd0ions(eg2AaenStxgugediyofiourpym0so.im0v3oml(ogAnuetswhgmrosnotkuuedny,,s.TohroM3mafl0eomradendogfodalm.ya((s1cueydnxiorcmoodlugdprnatmP,oFnO1k9Se9r8is)m ChaepsmloenskeplyascewdedrireocbtsleyrivnetdoattheJsathomdacah.y fTohreemnoenkaeylsiwaeisghSeide3.n1.d2.bokdgyowshye iwneieorne.codfieadppveiares 0 ENVIMRD(002)1 FINAL bweeegkilnyn.ingHeomfattohelosgtiucdayl aatnddacylin-i7ca(lbacsheelmiinsetrvyalauensa)lyasnesd wdearye2c9o.nduAcdtdeidtoonnalsabmlpoloeds scaolmlpelcetsedfobrefcolrienitchael tcahkeemnisotnrydawyesre7.c2o,ll3e,ct7e,d1o4n,asntdud2y9.daIynsa2d,d7i,tiaonn,d s1a4.mplBelsofordomsadmapyles-7faorndsedrauym2P9FwOeSrecoanncaelnytzreadtifoonrslewveerles aonfimesatlrsadwieolr,e etsetrrmoinnea,teedstraisols,chtehdyurloiedd astti3m0uldaatyisn.g hAotrmnoencer,optrsaiyiosdaotmhpylreonoifnel,ivearndwatshycroolxlienc.tedTfhreomsetaucdhy faonrimparolliffoerrpatailomnitcoeyllnCuocAlaorxaindtaisgeenacetviavliutaytiaonna.lysAes.samSpalmepolfesliovferlwivaers, tlessotsc,olalnedctpeadnfcrroemascwacehreacnonllaectfeodr mPiFcOroSsccoopinccaelnltyr:atiaodnreannaallsy,sise.ye,TKhiednfeoyl,lolwivienrg, olrugnag,nssfprloenm,copnatnrcorlesasn,dfaellmotrraelatbedongeromuaprsrwowe,reteesxtasm,inaendd thymus, oNbosnerevaotfiotnhse, mboroidkyeywseidgihetd,beffooorde tchoenssutumdpyiowna,s benoddeyd. temTpheerraetuwreers,e nhoematetsotlroeglyt,edoreffmeacctrsosocnopcilci.nicoalr smtiicmruolsactoipnigchpoartmhoonleo,gyanfidndtihnygrso.xinN. otCeesltrperloalitfeedraetfifoenc,tsawsemreeansoutreedd ibnythiemmluevneolhsiosftoscthreomni,caelstrdieotle,cttihoynroiodf proliferating cel nuciar antigen, was no increased in the liver, teste, of pancreasofmonkeys. v"Tahleuemsonaknedyscoinntrtohles 2a.t0 mdea/ykg2/9d.ay Sgirmoiluaprlbyo,ththsehomwoendkeesytsradiinolthleeve2l.s0thmaytkwge/rdealyessgrtohuapn tbhoetih psrhesotwueddy trrieoadtmoetnhty,rotnheinheiglhe-vdeolssethmaotnkweeyrseallessothhaadnttrhieoidaptrheysrtoundiynevavlauleusesalnodwecorntthraolnscoatntdraolys2i0n. basHeolwienveesra,mpplreestahesweellh.ormSiannceeltehveelmcuhmabnegressaorfettesrteeadtmaennitmaellsataerdeefsfmeacltls.and baseline levels are variable, it i not clear if OInf t0he(6fimnaolnskteuydys,pTehromsefxo)r,d0.(02300(24)maodrmiikneiyssterpeedr sPeFx)O,S0t.o15cy(n6ommoolngkuesysmopnekresyesx)b,yorora0l.7c5apmseulkeg/atdadyos(e6s amsosnikgenyeds1p0e:rrseecxo)vefroyr g2r6ouwpeeakns.d weTrweonoatnirmeasltsedffroormattlheeascton5t2olw,ee0k.s15foalnldow0i.n7g5thmg/lkagt/addamyingirsoturpastiowneroef dPaRiOlSy.forAnaibmnaorlmsalwietrieesobasnedrvseidgntswiocfetdoaxiilciytfyo:rfmooordtacloitnysuamnpdtmioornibwuansdiatsyseasnsdedwequraelietxataimveilnye.d aOtplhetashtalomnicce `reexcaomridneadtiwoeneskwleyrebedfoonree tbheefosrteaitniotfiattrieonatomfetnrte,atomnenDtayasnd1duarnindg1weaenkdsw2e6ekalnyd t5h2.ereaBfotedry. wBeliogohdt daantda uwreirnee tsraemaptlmeesntwaenrde actolslpeecctiefdiedforintcelirnviaclasldohreimnagtotlroegayt,mecnltinaicnadl rcehceomviesrty.ry, Balnododursianmapllyseiss wbeerfeoraeltohetaskteanrt foofr 1H0or3mMonfeordeatnearlmyisniastioofnsP. OSSamplelveelss.of sTehreumfowlelroewicnoglloercgtaendsatwvearreiowuesitgihmeedpoaitnstcshdeudruliendthaendstuundsycahneddusleendt osavcarriyfi(c2e)s,; ppaanicrreedaso,rgtaensstsw(e2r),eawneditghhyerdoisdep(a2r)atweilty:h paadrraetnhaylroi(d2)., Tbrhaen,foelpliodwiidnygmtiisss(u2e)s,wkeirdenecyoll(2e)c,teidvefro,r ehiysetsop(a2)t,hofloegmyu:r waidtrhenbaolsne(2m),arsroorwt,, gbarlalnb,ladcdeecru,m,heacret,rii,leucmo,lojne,judnuuomd,enKuimd,neyespi(d2i)d,ylmeissio(n2s),, esophagus, iver, lung. [mmaamnmdiabruylagrla(n2d),. msecsiaetnitcerniecrvel,ymspehminnoadle,veosviaclrey ((22)), spkaenlcerteaals,mupsictulieta(ryt,higphr)o,stastkei,n,respcitnuaml, csoarldiva(rcyervgilcaanld, pthaorraatchiycr.oiadn,dtrlaucmhbeaar,),ursipnlaereyn,bslatdedmeru,m uweirtuhs,bonaendmavrargoiwna,. stoLmiavcehr,stpeestciisme(2n)s, tfhoymmus,theth0y.r1o5ida(n2d) 0w.i7t5h mike/day recovery animals were collected via biopsy and analyzed for PFOS levels. wMaeleekss woefitgrheeadtm3e.n3t-,3.m4aklgesawnedifgehmeadle3s.7w,ei3.g8h,ed3.52,.8a-n2d9 3k.g3 akethfeorbetghienn0i,n0g.0o3f,th0e.1s5tudayn.dA0t.7t5hemegnrdkogf/d2a6y mtriekat/mdenatygrroeuspusmreenspegcrtoivueplsy.reFsepemcatliveeslyw.eiTghheeddi3f.f1e,re3.n1c,e 3b.e1rwaenedn2t.hekceonftorrolthean0d, t0.h0e3,0.07.515mgakndg/0d.a7y5 3 ENVAMRDRO02)I FINAL wfeeimgahlte btertewaemeenntthgercoounptrsolwaasndsttrateiastteidcaalnliymsailgsniwfiecraent.noAltontgheer oebnvdiouosf.the recovery period, differences in "doTswionmgaolnesDfaryom15t5he(0W.e75ekma2/3)k.e/Cdlianyicgarlouspigdnisdnnoottedsuirnvitvhiestaontihmealscihnecdluuldeedd:saccornifsitcrei.ctOendepuapniilms,alpadlieedguamfse,r dfeewh,ydrmautciooind,,anldiqruiedcuamnbdenbtlapcoksi-tcioolno.reIdn afdedciest,iono,wthefoaondimcaolnswusmptciolodn,fohtyhpeoatcotucihv.tyA,nlaebnolraerdgerdeslpvierratwioans. dientfleacmtoevdatiboyn.paOlpnatdioany. 17C9a,usthee osfecdonadthmawlaeswadsetsearcmriifniecdedtion baemopruilbmuonndarcyondnietciroons.isClwiintichalsesviegrnes naoctuetde icnaculsuedoefddelaotwh fwoaosdpcootndseutmeprtmiionend,.excessive salvation, labored respiration, hypoactivity and ataxia. The Mthaele0s.1a5nadndfe0m.a7l5esmgin/kthge/d0a.y75grmogu/pksg/hdaadyldoowseer-ghrioguhpdehnasditlyowleiprotportoatlecihnoclhesotleersotlroalnddumrailnegstarneadtmfeenmta.leTshien etfhfeechtigohndtootsael cghrooluepstwereorlew6or8s%enaenddw4it9h%tilmoew.erB,yredsapyect1i5v2e,lmy,eatnhttottahlancholelveesltserionltfhoer mcoanltersolanadnifmaelmsa.leTshien defefnescittyonlihpiopgrhotdeeinnsilteyvellispowperroete7in9%waasndgr6ea2t%erltohwaenr,thraetspseecetnivweiltyhicnhmolaelsetesroaln.dOfenmadlaeys 1f3i2o,mtthheemheiagnh dhoisgeh `tortoaulpbitlhiarnubitnhecyonwceernteraitniomnasleanadndhifgehmearlseercounmtrboilleaanicmiadlsc.oncMeanltersatiiontshethhainghmadlosseignrcoiutphraltshoe hcaondtrloolweorr oeftfheecrt torneahtimgehntdegnrsoiutpys.lipoTphreoteefifneccthoolnestteotraoll cwhaoslesrteevreorlsewdawsitrheivner9sweedewkistohifnre$cowveeerky.s of recovery and the vEasrtiraatdiioonl ovnallyuesthewedraeyl[o8w2ervainlumeawleass gsiivgneinfic0a.n7t5. mEgs/tkrgon/edavyalounesdawyesre62g,en9e1r,alalnydhi1g8h2erbuitn btheecoturseeatoedf sfiegmnailfeiscanotnlyddaiyfsfer3e7n,t 6r2oamndthe91cobnturtolasg.aiEnxcbeepctaufsore moaflevsariiantitohne 0i.n1t5hemgd/aktga/dnaoynegroofupt,hetsieodvoatlhuyesrowneirnee vTahleureeswweerreeostihgenrifiincsatnatnlcyesloiwnewrhoinchdaHyosr9m1neanvdal1u5c2siinn mtraelaetsedagnrdoufpesmawleersegdiivfefner0n.t15faonmd t0h.o7s5eomfg/ckogn/tdraoyl.s boutt haepspeeadriftfoerbeencereslawteerdetnootthceonasdimsitennitstorvaetriotnimoefotrhebettewsetemnatseerxieals., wAeprpeanroetntcledairflfyerdeonscee-sreilnattehdeasnedxduiadl maturity ofboth males and females used inthe study complicateth interpretationofthe hormone data AToc-tbeordmyinwaeligshactripfeicrec,enfteamgaesl,esainndthleiv0e.r7-5to-mbyr/aking/wdeaiyghdtosrea-tigors.oupInhmaadleisn,creliavseerd-taobsboolduytwIeviegrhtwepiegrhcte,ntlaivgeerswhiegrhe-dionsceremaasledesinantdhenhoinghe-dhoisgeh-gdroosuepfcemoamlpe.arOeFdthtoe tthweocomnatrloelss.not"Msuortvlievdin"g vunetrils twheersechoebdsuelrevdedteirnmitnwaol ssaucrrviifviece,toonsechheaddulaed"msoatctrliefidc"e)anhdadlarcgeentlriivlero.bulTahrreoerofdif4fuhsieghh-edpoasteocmelalluelsar(ihnycpleurdtinrgopthhyostehatthatwadsidanlosto ofbesmealrevsednidn a2llohfi4gmha-ldoesseinfetmhaelehsi.gh-Cdeonsteriglroobuupl.ar or diffuse hepatocellular vacuolation occurred in 2 of4. NinodicPaFtiOnSg trhealtatthed elfesfieotnsssweenreatotbesremrivneadlsaeictrhiefricmeamcaroysbcoepirceavelrlsyibloer. microscopically at recovery sacrifice StheerpurmesaenndceoivferPFsOpSe.cimSeenrsumcowllaesctheadrvfesotmedtefsrtoamnbilmoaolds twheartewassencetnottihfeg3edMwLiatbhoirnaotnoeryhoaunrdoafcnoalllyezcetdionfo.r sLeitve1r0 smpaeinctiamiennsspweecriemelnasshatfr-o6z0en0in8l0iquCidunnittilrosgehni.ppBeodtthoitvheer3aMndLsaeb.ruSmasmapmlpelseswewreereshsitporpeeddifrnozferneeaznedr oconvedrreydctehefrionmifCeovpahanscee oLfabtohreatstourdiye.s Otonc3eWrepecreiiovdeidcaaltly3MfrosmpeAcuigmuesnts w1e9r9e8 stthorroeudghinMfarrecezher20a00eitwhheirch 2 ENVAMRD(2002)17FINAL 55C + 10:20C or 20C + 10C. cDoulrleicntged.thOeFftirhset 2s6erwuemeskpsecoifmetnhse, s1t5u1dywearetoftarlomofGr5o5u0ps1e,r9u9mfsrpoemciGmreonuspa2n,d15320flriovmerGsrpoeucpi3menasndwe1r48e ffrroomm GGrroouupp 44.. EIinghtthlirveercosvaemrpyleGsrowueprse,c7o2llescetreudmfraonmdG4roiuvepr1,sampflreosm Gwerroeupco2l,le1c2tefdrofmroGmroGurpou3pan1d: 7124 Serum and 4 liver samples from Group3 and 80 serum and4 liver samples from Group 4. LLiivveerr asanmdpsleersuwmerseamhpolemsogweenriezeedxtrparciotredtouseixntrgacatnioino.n-pSaarmipnlgereexatgreancttsanwdermeetahnyall-yrzretd-ubsuitnygchhieghr-p(rMeIssEu)re rleisqpuoinds_echmroodmea.togPrFaOpShlye-vcellecstwreorseprqauya/natintdaetmed mbaysesxtesrpneacltsrtoamnedtarryd c(alHibPrLatCio-n.ESMSMS) in the. multiple wLievreer sspaimkpeldeswiwtehreTHhoPmFoOgSeniazneddeixntrwaactteerd. uAsinngaliaqnuoftoonf-pcaarcihnglievxetrrhacotmioognenpraotceedaunred.all Asnerufmons-apmaiprliensg. erveaagpeonrtatwedasunatdildeddrytoonthae nsiatmrpolgeesn eavnadpotrhaetoironanpdairtshewnewreereparrteictoinosnteidtutiendtoiMnIB1.E0.mL.Thoef meexttrhaacntoslwaenrde passed through'a 0.2 um nylon filer, "chTahreaactnearliyssteisc woefrtehepeMrufoorromcehdembiycamlonoiftoirntienrgesotnuesionrgmHorPeLCp/roEdSu/cMtiSoMnSs.selMeoclteedcuflraormiaonsi4n9g9l,e pthreimparriymairoyn qiuoanntfiortaPtiFvOeSan(alCy,sFi,s;S0;) analysis, was fragmented to produce fon 99 (FSO). lon 99 was monitored for AwletrheosuigghnilfiocwanltelvyellsoowferPEthOaSn wtehorseeofftoeunnddeitnecttheedlionwthdeosseerateastndaniimvarls.of tPheFOcoSntlreovlelasniimnaltsh,e tshesaeolfevteelsts aannidmaflesmailnecsreraessepdecwtiitvheldyoastetdhuerienngdtorfeaWtemeenkt r1o(m0 129140+=941.57aanndd 1206.40 ++223.711gugm/]mliinnmtahleeGsraondup4femmaalleess roevsepretcitmiveeluyntiinl Gtrheoyupre4aact htheeden41.do+f2W5.e9epkg27m. iDnumrailnegsraencdov4e1r.y4, =PF1O.1S5 lmevells iinnsfeermuamlessafmrpolemsGdreocurpea4seadt a7n9dwefeemkaslepsosatnidre0at.m0e2n1t5. +C0o.n0t0r2o9l6vaalnudes0.w0e3r4e3<+ L0.O0Q03(5t5hegl/imimtoifnqumaanlteistaatnidonf)emstalWeeserkes4peicntibvoetlhy mataltehse ebenldowo.f WeTheekre79.weTrheensoersuigmnivfailcuanets fdoirffseerleenccteesd bweetewkesenofPtFrOeaStmelenvtelasndinretchoevesreryaaroefsthreoawtnedinmatlheestaabnlde females 5 r Ci (i fr[r rom fo [Mules [Female [Males [Females |Males |Females |Males | Females | rm TR = li rs I ENVAMRD(2002)17FINAL dweearthe.coFlilveectaendimfaorlsm'isteoxchionntdhreiatlreacattimveintty,ghreopuaptsocweelrluelasracprriofliicfeedradtuiroinnrgatw,eeaknsd 4d,et1e4rmainndatSi3o;n oivfepralsmaimtpolyelsCcoonAcenotxriatdiaosneofactPiFviOtSy. inSefirvuemr aannddselriuvemrdusrpiencgimaenndsatwtehreeencdoollfetchteedinf-olrifeanpahlaysseeosftohfetshteudyp.resence and i`Tnhcerreeaswesasinassuirgvniivfailcainnt minicdrheiagsehd(5t.0rendppimn)suarnvdivhaligthh-adtooscec(u2r0r.e0d pinpmt)hegmraoluepss tahsatcowmapsardeude ttoo sthiagtniofficatnhte scuornvtirvoall.grNooupS.ignNiofniceantoftrtehned wotahsernottreedatiendsugrrvoiuvpasl iinnfetmhaelemsa.leTsherreewveaalseadsiagnnyifsiicgannitfdieccanrteadsieffienresnucrevsivailn icnomtphearmeidd-tdootsheat(o2f.t0hpepcmo)ntgolr.oup and not in the mid-high (5.0 ppm) and high-dose (20.0 ppm) groups as dMuarliensggwieveenks209ptphmrohuagdh 3s7i.gnifFiecmaantlleyslgoiwveernm2e0anpbpomdyhwaedisgihgtnsificcoamnptlayreldotwoeranbiomdaylsweiingththscconotmrpoalrgerdou1p0. asnuirmviavlisnginmatlheescoanntdrolfemgarloeusp wdeurreingnotweseikgnsif3icatnhtrloyugdhiffe1r0e1n.tAftrowmeetkhe 1c0o5n,tromles.anFobooddycownesiugmhpttsioonf tfhoer mfoarletsheanhdigfhe-mdaolseesfweamsalseismiwlharicihn ahlladecsautteidsgircoaulpyssciognmipfaicraendtltyolaonwiemralfsogoidvecnontshuemcpotnitornoldmuartienrgiawlexeckespt2 through 44. AiUncrtehaesewdeienkth1e4maanledswgeievken5230 ipnptme.rimInsfacermiaflieces,gaibvseonlu2t0epapnmd, roenllaytivtehllivievrerw-eoi-gbhotdsy wweeirgehtsipgenrifciecnatnatgley wmaalsessiggniivfeincan$tloyr i2n0crpeapsmed.anTdreianttmheentf-eemlaaleesd ghiivsetnom2o0rpphpomlo.giTehechcahnagnegseswecroensisseteend ionfthhyepelrivterropihnytohef hvaecpuaotloactyitoens. iTnhecenitnrciildoebnuclearaanrdeassevienritmyaloefs tahnedcfheamnagleess,teannddedmitdozohnealgretaotecrenitnriltohbeulmaarlehse.patDoiceyttaircy cahdomliensitsetrroaltfioorn moaflePsFaOnSd ffoermaalpepsrfoexdim2a0teplpym;53anwdemeiklsdlwyahsigahsesroacliaantiendewaimtihnomtirladnslfyertaosemofdoerrmaatleelsy fleodw2e0r pTnpemp.atoIcneltuhlearuncsecnthreidluolbeuldarsahcyrpifeirctersopbheyt,wceoesninWaepehkislc5h4epaantodcy1t0i5c,garnaniumlaelss, gainvdencen2t0riplopbmulahradheipnactroecaysteidc pigment were noted. Increased hepatocellular centrilobular hypertrophy wasseeninanimals given Spp. fAiundtihngst,erimnicnlauldisancgreinfliacer,gethde,lmiovtetrsloedf,adniifmfaulses dgairvkeenne5do,ror2f0opcaplmlyexlhiigbhitteended. lHiegphattointcorxeiacstey,incmhaacrarcotsecroipziecd rbyansiign,ificceannttriilnocbruelaasreshe(pPa<t0o.e0y3i)c piingmceenntt,iloobrucleanrtrhiylopbeurltarrophheyp,atcoecnyttriiclovbaucluaorlactoisoinnowpahsiincotheedpaitnocmyatliec ahnydpleorrtrfoepmhaylewarsasalgsoivoebnse$rvoerd2i0n pmpimd.-dosAe (s2ignpipfmic)anmtailncrraetas.se (SiPg<n0i.f0i5c)antininhcerpeaatsoecselilnultahre ciennctiidleonbcuelaorf yhsitsilceshieopnatioscbeellluilvaertdoebgeenderatutiooonedwaasgfeooufndthinaanlimtahlesmnalde itsrenaottedcognrsoiudpesre(d0t.o5b, e2,5,troeartm2e0nptpeml)a;tehdowever, cBoansseiddeornedth1e0pbaetho0l.o5gipcpalmfiinndmianglseirnattshealndve2, tphpemnoi-nobfseemravleed-raadiv;ertshee-elffoewctoblseevrevle(d-NaOdvAeErLse)-cffofrePctFOeSveils (LOAEL) is 2 ppm in male rats andS ppm in female ats, "iTnhcerreeasweassinnocelelffperctoloinfehreapattiaiocsnpmaelamsiutroeydl-bCyoAprooxliidfearsaetiavceticveiltly.nuTchleeraerawnetriegeanls(oPnCoNsAt)atiasttwiecaelklyssi4gnainfdica1n4,t or by bromodeoxyuridine (BrdU) at week 53. aFodrennoeompalaisntimcaleeffreact.tsTa,hsiisgnwiafsicadnuteptoosaitisviegntirfeincdan(tPi=n0c.r0e2a7se6)(Pw<a0s.0n5o)teidn tihnethheigihn-cdiodseengcreosoufphe(p1a1t.o7c%e,ll7u/l6a0r) 3s ENVAMRD(2002)1 FINAL ofveorlthle icceolcntauodllena(o0mr%a, i0n/t60h)e.hiAgh-sdiogsneifirceacnotvleyryingcrroeuapse(d23i.n1c%,ide9n/c3e9)(wPh<0e.n05c)omwpaasredobtsoterhveedconftorrolthgyrroouipd (c5a%r,cin3o/m60a).in thThehrieghw-daossealrseocoaverslyigghrtouipnc(r2e5as.e6%,in 1t0h3e9)coasmbcionmepdartehdyrtooidthaftololfictuhlearcocnetlrloladgreonuopma(10a%n,d 6/60); the increase significance relative doidthneothirgeha-cdhosseiagtrisotuipcal(8s.i5g%n,if5i/c5a9n)c.e relative to the control but did reach statistical (InP=t0h.e01f5e3m)alaens,d sciogmnbifiinceandt hpeopsaittoicveellturleanrdsadweenroemaobasnedrvceadrciinnothmea i(nPc=i0d.e0n0cSe7s).ofThheepsaetoccaeslelsulwaerraeddeuneomtao s0/i6g0n)i.ficAanstiginnicfriecaasnets iinncrtehasehi(gPh=-0d.o0s4e7g1r)ofuopr (c8o.m3b%i,n5e/d60t,hyarnodid1f0o%c,u6l/a60r)caellcaodmepnaormead atnodthcearccoinntoomla(w0a%s,. ofborservtheed initghhedmoisd-ehigghro(5u.p0 pp(mw)higcrhoupsh(5o%w,ed3a/ass5cloigmh0ptar)deedcrteoastehe coinntroilncgirdoeunpce(s0%,of0/60m).amEmxcaerpyt tfubmroorasdewneormealoabdseenrovmead ainndalclotmrbeiantemedntmagmrmouaprsywfhiebnroacdoemnpoamraedantdo ctahrecicnoonmtrao)l,s.inScirgenaisfeiscainnt mianmcrmeaasreys (fPb0r.o0a5d)enomainlademnaommmaaarnyd cafrbcirnoosmdaen(o7m29a%/,ad3e61n5o0m)awer(e60ob%s,erve3d0/i5n0)th alonwd-dosceom(b0i.n5epdpm)mgarmomuaprays cexoimipbaitreedd oa stwhisrtecspaelcltyivesciognntirfoilcasnt(3(8P.<30%.,052)3/i6n0craenadse48(.634%.,6%2,9603)1./4T8)heimnidth-edosinec(i2d.e0ncpepmo)fgcroomubpianlesdo mmaammmmaarryy tfuimborrosaidnentohemam/iadd-ehniogmha/(c5aprpcmin)omdaoseovgerroupthdeidcnoonttrroelacghrsotuaptist(i4ca3l.8s%i,gnif2i9/c6a0n)c.e Increases relative to in the control 36 [a -- Thcuingeikydaa FOS i trsummed Tl. Table.Summary of carnegie dtofPEOS fn rat, Tow fr hre sa || Ooo [3 |SooemToo [S| [TMoPoeT|r vio | | dsno | cto | cSmo | cm | tm d[wne || dwen|| asah|| deSs || me rir con || dwGEne] | iGGRRn | wi| cin | tiGloeo || dd|giliey|||| adaami || |e deoien otenwirnn ||roe 0t|e | rd3e ICn0 --||----aIl0 || IESdxm hsT pooi T) cSaE | oRwEom ||I eai]n | I aamm | a|deo dioiky MSmete a' |||| wat0Gowe|||| aod0oio|||| eo0ald|||| douahwyh d,ibhy oto cm oto || aon |eol || g8%e A tetm || aw[sy|G| GGwone||| ave| | lcwalxy)i||| dasaiaon do5di=inn comma | wRSo|| cEiOn | G|iGn|| a4vin aole PScmipyemoma vS 8<e h0epe re <005 Sram and irePROS Ummnderoaheeccotntlioos ofochoodenptosdiis,.PoOhDSA(FsTOsSSccT4eh99en, doio sheesvussmeofanPdEdOhNoecerofarshEdSeersdcith = ENVIMRDI00ENAL [T=] =Tor[oo[owTom] Table4. SummaryofPEOSConcentration-(Ssegrtum) T to T| odmwoyr JJeo[Pmeea]5]0]eseamste50||| rTems0||oaeanen rom[rar ora eeye | w|re iin oag iwnia|| wooww|| weays ewnrs T[eeym | maan || wse m roam: woes was Sway Tfoyo wr etios woremmon] oi | re| mn | le w= 10) o=1) 0-25) 0-2) OE EE| ty | Tonr E TTTT I TeesFer} SFearn oscm r e te utt ayafneshodon d S t -- c a semiieeesgisto ot otose tttcTTohey JEREaEs m] So I rdIll re I fe 092177 ot 018540188| 1294681 1314614 Ea E EE ------i ------ iid 1294104 ] ENVIMRD(Q002)1 FINAL Shiiggnhs-doofsemagtreorunpalofto1n0icmigt/ykcgo/ndsaiys.tedNoofsiogtnhiefrisciagnntsorfedmucatitoensrintomxeicaintybwoedryewreeipgorhttesd.duUrnidnegrGtIhDe12c-o2nd0iattiotnhse oifnditchaetesdtudy, a NOAELof mgkgiday and a LOAEL of 10 mgkgidsy for matemal toxicity were iDmepvlealnotpamteionntaslitest,oxciocritpyoraevliudteenat, raetsor1p0tiomngs/itdsaaynd ctohnesimsetaend noufmbreerdsucotfiovnisablien mtahlee,mefeamnalen,uamnbdertotoalf fneotsussiefsi.edbu,tastshyemedtirfifcearle,nbciepsarwteitree, nnodt smiitssstiincgalsltyemseibgnriafeicwaentr.e oIbnseardvdeidtioinn,allunduossuealglryouhpisg;hhoiwnecindeern,cetshesoef nskoetlectoanlsivdaeriraetdiotnosbweertereaatlmseonot-brseelraveedd. inTchoentmoolstfentoutsaesblaetstihgensoafmdeervaetleopamnedntthaelretfooxriecitthyeosebseefrfveectdsiwnearlel dporospeorgtrioounposfcfoentsuissetsedwitohf athbenolremnasliatbineosrmoaflitthye ilnenosneoforthbeotehyel,enswehsicwhaswsaisgnnifoitcasnetelny hiinghceorntri oltsh.e hTihgeh adlotsheouggrohupa. vaAriltyeyoefambonroprmhaolliotgiiecsalapappepaeraerdantcoesbewelrocealpirzeesdentto twhiethairneathoaft tlhoecaetmonb.ryonAaclcorfednisngnuctloeutsh.e `camubthroyrso.nahlisleanbsnonrumcalleuist.y apSpeecaorneddaroy bleenasnfairbeersdienvedleovpemleonptmepnrtogorfesthseedprniomramraylllyensexfciebpetrsifmomremdiinagtetlhye dseuvreoluonpdmienngtalthteoxiacbintoyromfal1meymkbgr/ydoanaylwansucilneduisc.atedU;nddeervetlhoepmceonntdailtiNonOsAEofLtchoeulsdtundoytb, e4esLtaObAliEsLhedfor aIdnmianissetceornedd 0p,re1n,at5,alanddev1e0lompgm/ekngt/adlaytoPxiRcOitSy isntucdoyr,n ogrioubpysgaovfag2e5 opnreggensatnattioSnprdaaygsue(-GDDa)w6l-e1y5ra(tWsetwzeerle, m1a98t3i)n.g oSrexuunatilllytwmoatwuereekSsprhaagducel-aDpasewdl.ey Mraatts,inognewapsercsoenxfipremredcabgey,dawielryevpaagiirneadl uenxtaimlicnoantfiiornmsatfioornthoef pwraessednecseigannadtevdiaabsilditay0yofosfpegersmtaotiront.he pDroesseesncweeroefaadcjoupsutleadtaocrcyoprlduign.g tTohethedamyoosftcroencfeinrtmlaytrieocnorodfedmabtiondgy. wdaeiilgyhtfomecalsiunriecamesnitgsn.s ofDtoaxmicsitwye.reIondbisveirdvueadl tbwoidcyewdeailgyhtfsoransdigfnosoodcfomnosrutmapltitiyonanwdermoerriebcuonrddietdyoanndGoDnc6e, b8y12c,es1a6r,eaannsdec2t0i.onAonnimGaIlDs2w0.ereA sgarcorsisfinceecdroonpsGyIDwa2s0pbeyrfCoOr;meadspohnyaxliladtaimosn. anTdhteheutfeertuussefsrowmereeacdhelfievmearleed fwiavse aenxcdisdeeda,dwfeetiugshese,dnaunmdbeexraomfincaerdlyfaorndthleatneurmebseorrpatinodn,plaancdemaennytaobfnoirmmpallainttiaetsioanndsitths,ennwuemibgeherdaangdaionf affetmraltehewacsonteexnatmsinweedrebryemgorvoessd.necTrhoepsyo.variEeascwherfeetuesxawmaisnesdexfeodr, twheeignhuemdb,eranodf ecxorapmoirnaedluteexat,ernaElslcy.h aAbpnporromxailmiatiteeslybyonteh-ethiWridlsoofn ttheechnfieqtuues,es wwiethrepafritxiecdulairn stBioeunitno'ns 1olutthieoneyeasn,dpaelxataem,inaendd fbroarin.visceTrhsel broenmemsa.inivnegrtfeebtrualsescowleurmen,surbijbecctaeged,teoxtaresmkietlieetsa,l aenxdamipencattoiroanl tahnadt pienlcvliucdegdiredvleasluautsiionng oalfitzhaeriskruelld,; bloonneg alignment and degreeofossification were also evaluated, Efovlildoewnicneg otfremaattmeenrtalantdoxciocnitsyi,detrhaetd wtoabseotbrseearmveendt-atretlhaetd5,acnodns1i0stmegd/ofkgh/udnacyhdeodsepogsrtouruep,sabnootrhexdiua,ribnlgoaonddy vbaogdiynawlediigshcthagragien,sudtuerriinnegsGaiDn,6-a5l,op6e-c1i6a,, arnodug0h-h2a0irwceoarte, aalnsdobolbosoedryvecdrusatt.tShiegnSifaicnadnt1d0ecmrge/aksge/sdianymdeoasne rroeupast. haTnhecsoentrroeldsucdtuiroinnsgwteherepocsotnesxipdoerseudretopebreitorde(aGtmDen1t6-2e0l).ateSdigsniinfciecamnteadencrbeoadseyswienimghetangationtaslwfeoroed bcootnhsutmhpetaionnd1we0remgo/bksge/rdveady doonseGgDro1u7ps-.20Tihnetmheela0nmgrga/viddautyeridnoesewegirgohutp,iatnhde o10nmGy/Dkg7/-d1a6ydaondse0-g2r0ouipn wutarsinsiegnwiefiigchatntslywelroewelrowwehrenincaolmptraeraeteddwgirtohupcso,ntwrioltsh.siTghniefimceanatndteecrrmeiansaelsbaod5yawnedi1g0htmsg/mkign/udsayt.he gHriavgihd "0 ENVAMRD(2002)1 FINAL dwoesree aonbismearlvsedalisno perxheigbniatnecdyarnatien,crneausmebdeirncoifdecnocrepoirnaglaustterao,inatensdtinnaulmbleesrioansn.d pNloacseimgennitficoafntimdpilfafnetraetnicoens GsiDtes1a7.monUngdterrattheedcaonnddictoinotnrsoolfgtrhouepss.tudTy,wao NdaOmAsEiLn othfe|1m0gmkgg//kdga/ydaaynddoaseLgOrAouEpLwoerfe fmoguhnkdg/ddeaayd foonr maternal toxicity were indicated. rSeisgonrsptioofnsd,evoetlaolprmeesnotrapliotnosx,icniutymbienrcolfudeddeaad fdeotsues-erse,laatnedd ftetraelndlostso,waalrtdhoaungh,inncorneaeosefdthinecsiedeenffceectosfwelraet.e mstaalteisstiacnaldlyfesmiaglniefsicwaenrtelyobdsifefrevreedntinftrhoemaconndtrol1s0.mSgig/nifkicdagnots/edegdcrroaeuapsyse.s Tinhemepearncefnettoalfmwaeligehtfsetufsoersbwoatsh. s5i2g%ni,fi5c4an%t,ianncrdea6s0es%infoirnc1,om5,plaentde c1l0osmugr/ekgof/tdhaye,srkeulslpewcetirveelayb,secrovmepdarinedthteol4o4w%ainndchonitgrho-lsd.oseSgtartoiustpiscablulty cootntianintihneg mfietdu-sdeossweitghrovupi.scerSatlatainsotmiacallileys,sigdneilfaiyceadntosisnicfriecaatsieosn,inatnhdesikenlceitdaelncveasriaitniotnhse wneurmebeorbsoefrvleidteirns sthuebchuitgahncdoousse gerdoeumpa,ofan1d0 mcgrykpgl/odracyh.ismThaessewelilnclausdeddeleaxytserninalskaenldetavliscoesrsailfiacnaotimoanlioefs tohetfhskulcll,eftpepcatloartael, iwerrie,alrsiob ocbasgee,rvveedr.tebUranldecroltuhmen,copnedlivtiicongsirodfle,thaensdtuldiym,bsa. NSOkeAleEtLalovafr|iamtigo/nksg/indatyhearnidbsaanLdOAstEeLmeborfac5 mekg/day for developmental toxicity were indicated o`Tbhseerdveevdelionptmheent1a9l53edyeevealbonpomrmeanltiatlietsritchiattywsetrudeyseeveenn tinhotuhgehptrheevsitouudsysdteusdiygn(aGonrdtdeors,es1w9e80r)e twheerseamneo.t dFeitnedrinmgisneodftoabbneoramrailficttisesofsiencteiyoenidnegve(l3oMpmCeonmtpawneyre, 1in9i9t9i)al.ly thought to be treatment elated but later iLnau0.e5ta%l.T(w2e0e0n1-)2a0dmbiynigsatveargeed Sbpergiangnuien-gDaownlegeystraattsioannddaCyD-21anmdicceondtoisneusinofg0,unt1i,l5teorrm.10 Hmaglf/odfatyhePdFaOmSs. twoerdeelsiavcerr.ificPerdeolinmigneasrtyatrieosnudlatsy2ar1e a(rvaitlsa)obri.gestIantiraotns,dtahyee17 w(amsicae)siagnndiftihceanrtemraeiduncitnigodnaimnsmawteerrenaalllboowdeyd mwekigg/hdtayga,inbuatt l$ivaenrdwe1i0ghmtgs/wkge/rdeayc.ompaMraatbelrealocsoenrtruoml.choAltes1t0romlglakngd/datryi,gltyhceerreidweasswerreedurcetdiuocnednatfet1a0l abfotderybwiertihghatl atnhedpaunpsinicnretahsee1i0n mclye/fktgp/adlaaytegarnodupandaiseda,rcaan.d 9Al5l%puopfsthweepruepbsoimn talhieSve,mgbu/tkgw/idtahying4r-o6uphoduiresd iwnictrheianse2d4atho$ursa.nd 1In0 mmgi/cke,g/dmaatye:rnsaelrubmotdryigwlyeciegrhitdeswawseruenarfefdeuccteedd waenrde leilveevratweedigathtSsawnedre10Simggni/fidcaanyt.ly `"Tchoempianrcaibdleencteoofcofnettraoll.morHtoalwietvyewra,snseloingahttally ibnocdryeasweedigahtt1s0wmegr/ekgr/eddauyceadnddumreianng tfheetafibrostdy3 wdeaiygshtosfwleifre,e mAdedcihtainoniaslmsftourditchse naeroenuatnadledrewaatyh.10 further elucidate the dose-response relationships and to examine the C1r0.i2s.t5ian0ret3.al7.5(m1u9k9g92/)daaydmiPnFiOstSeriend0.pr5e%gnTawnetenN-e80wbZyealgaanvdagWehiotnegersatbabtiiso,n 2d2aypser7-g20r.ouApd,odsoseesvooflu0,me0.o1,f o5bsmeirvkeed twwaisceaddamiilnyisftoerrveida,bilaidtjy,usatnedd cdlaiinliycalonobstehrevabtaisoisnsowferiendrievcidouradledbo1 dhyouweipgrhitosr.to aTnhdeafdtoeersdowseirneg Mduarticngalthebotdrycawtemiegnhttpsewrieordearnedcorodnecdeodnaigleystdautriionngdathyes 0poastn-dtr6e-a2t9m;enotopdercioondsu(imcp.tigoenstwataisonredcaoyrsde2d0-d2a9i)l,y athbrdooumgihnoaultatnhde psetludvyi.c viOsncergaeswtaatsiocnonddauyct2e9d, atnhed dtoheesnuwmebreeroeufhcaonripzoerda; lautgeraosns ensecchroopvsayryowfatsheretchoorradceidc,. l"aTthee eusteorriptwieornes.exaTmhienfeedtufsoersnwuemrbeerweaingdhedids,trsiebuxteidonaonfd iemxpalamnitnaetdifonosr, elxvteerannadl daebnaodrmfaeltiutsieess,.andAlclarfeytuasneds were examined for visceral and skeletal abnormalities and the brain of one-half of he fetuses were free. a ENVAMRDQO0)I FINAL handcrosssectioned and examined in si. `iandmaidndiisttieorne,d tahesastaelmleitdeossetsuday iwnatshecmoanidnucstteuddy.inThwehincuhmbperreogfndanotesNewaws Z3.ea5l,a3n,d3 Wahnidte irnatbhbeitcsontwreorle, 0bl.o1o.d10s.am2p.l5esanwder3e.7c5olmleycktegdd, aayndgrtohuepsl,iverrewspaesctiwveeilyg.hedThaenddsoeecstiwoneerde. euTthheanfiezteudseosnwegersetarteiomnovdeady a2n1d. `10extahmeinSepdonfsoorrex(t3eVrDn)alfoarbnaonmalmyasliist.icsA.t hFiestutsiemse,nodnlpylatcheentlaivewerearnedpsoeorluemdpaenarlylsters.haAvlelbseaemnplreespowreterde (se3nMt Environmental Laboratory, 20014). Maantdernnianle dtooexisiitny twhaes3e.v7i5demngtkagt/ddoasyesaboofrt1.0edm.g/Aklgl/adbaoyrtiaonndssobcocvue.rreOdnoendgoeestiantitohned2a.y5smg22k-g2/8daanydgrwoeurpe. sccoannstidfeerceeds itnrtehatem3e.n7t-5remlga/tkedg/bdyaythgerosutpu.dyScauatnhtofresc.eswTheerreealwsaos naotsiegdniifnicoannet ainndcrtehaesee idnoetsheinintchiede1n0ceanodf 23..575mwmgk/gk/gd/adyaygrgoruopusp, ornespgeecsttiavteiloyn.daMyesan10-m1a3t,er1n3a-l16b,od1y6-w1e9igahntd g2a1i-n2s4.werMeeasnignbiofidcyanwtelyigrhetdugcaeidnsinwetrhee saltsuodyca(ldcualyaste7d-2f9)o.r theThrecraetmweants paersiiogdni(fdiacaynst7-r2e1d)u,ctpioonstirnamtemaennmpaetreirond(aldabyosd2y1-w2e9i)ghatndgadiunratdiuorninogf tthhee tpreeraitomdewntaspearlisoodsiignnitfhieca1n.t0l,y2r.e5dauncded3.i7n5thmeg2k.g5 /mdga/ykgg/rdoaupys.grouMpe. anMebaondyfowoedigchotnsguaminptfioont(hge/ikgr/deay)stwuadsy stihgeniefnitciarnetlsyturdeyducpeedriiodt(heda2y.s5 m7-g2/9)k.g/daMyeagrnoufpooodn gceosntsautmiopntidoanysw1a6s-19si,gn1i9f-i2c1anatnlyd 2r1e-d2u4c,edasinweltlheas3.f7o5r "mTyhfekLg/OdAayEgLrofuorpmoantergnesatlattiooxincidtayywsa1s3-11.60,m1g6/-k1g9/,da1y9-a2n1datnhde N21O-2A4E,Lawawesll0.a1smtgh/ekgen/tdiarye.treatment period. rDeesvoenlpoipomnesn,tlailvetodxeiacditfyetwuasses,evlitderntsiatzedaonsdessoefx 2ra.t5iomywekrgeidcaoympaanrdabalbeovaem.onTgherenuamdbearndofcocnotrrpoolrgaroluupcsa., mMega/ndfaetyal bgrooduypsw.eighTthe(rmealew,asfemalasloe aandsigsneixfeiscacnotmbriednuecdt)iownasinsitghneifiocsasnitfliycarteiodnucoefd itnhethset2e.r5nuamnd(3i.t7e5r laivteerragaevse)riangetsh)e,2m.5etaancdar3p.a7l5s m(ei/tekradaavyeraggreosu)psa,nadndpuabissig(nitfeicranatnrdedfuectatlioanveirnatghees)osisniftihceati3o.n75oftmhgekgh/ydoeidy group. The LOAEL for developmental toxicitywas2.5 mg/day and the NOAEL was 1.0 mg/kg/day. n3tMheEnsvaitreolintmeensttauldyoLfadbooreastoeruyth(a2n0i0z1e4)d.on gAelsltasteirounmdaaynd21,litvhere lsiavmeprlaensd s(ienrculmudainnaglytsheossewefrreomrepuonrttreedatbeyd controls) had detectable levels ofPFOS; the values are presented below: Average Concentrationof PFOS in Rabbit Liver and Serum by Dose Group. Dose group (nghgday) PROS cone. liver (gig) POS conc serum (ug/ml) 0or0 0123319 02076930 2150 3133 2a3s3s8 37s 16 889 QPuFaOlSitiinvcerleya,sedi.ncrTehaesilnegveclosnocfePnFtrOatSionasreomfuPcFhOhSigwheerreinftohuenldiveinr tshaamnplnesthoefselirvuemr,and serum as doses of ENVIMRD(002)1 7FINAL w"Tahsesneotvaalvuaeislasbhleo,ulidtbies vnoitewpeodsswiiblteh tcoauvteiorni.fy tItuewarsecsotvaetredyotfhatenbdeocgaeunsoeursadainoallyatbeelferdomrefteisrseunecse."maMtaetrriiaxl mspaiykebreebcioavseerdyhiingdhi.catTehsetohenlaycccuornacclyuosfionquparnetisteanttieodnitotbhe +lab3o0r%a.torIyt riepaolrstoinottheadtt"hPatFOliSvewracsonocbesnterravteidonisn the liver and serum ofal rabbits dosedwith the test article" (3M Environmental Laboratory, 20014). 37 Reproductive Toxicity rAeprtowdou-cgteinveeraftuinocntiornepirnodaudcutlitveanitomxailcsityandstuodny,dedveesliogpnmeednta1l0,tlesetamifonrg,tahendtorxeipcrodeufcfteitvse eofffecPtFsOiSn tohne pofefrspdroisneg,grwoauspcwoenrdeucatdemdiniinsStperreadguPeR-ODSawblyeygravaasg(eChartidstoisaenseotfal0,.01.919,960)4., 1F.i6v,eagnrdo3u.2psmoyfk35eiraatys pfeorrsseixx (waepekpsropxriimorat1e02l2aynddaydsuroitnag);mafteimnag.le ra~tTsrewaemrmeenttreaitnedmatlhreourgahtsouctognetsitnauteiodn,unptairlturointeiond,ayndbelfaocrteatiSoancrifice F0 Generation: vPaarleunetsalwearneimraelcsor(dFeOd)wweeerkelyobdsuerrivnegdthtewitcreeadtamleyntfpoerciloidniincamasilgensr.atsB;oadnyd wweeiegkhltysdaunridngfomoadtcionngsaunmdpttihoenn `darioluypdcuorinnsgistgeedstoaftitonw, asentds oonf fleacmtaalteiornatsd.aysOn1e,4s,et7.co1n0s,is1t4e,daonfdthaet fsarcsrtitfeicnefienmafleemarlatesrwais.th cEoancfhirdmoastaigoen rofemmaaitniinngg ftehimsalgersoucpomwparsisdeodsethdeusneticlongedstseattiwohnidcahyde(lGiDve)re1d0naantudradlellyi.veDruedrivniga tChaees2a1r-cdaany-sleaccttaotniionng.periTohde, wthaes deavnasluawteerde aetvlaelausatttewdicfoerdcaliilnyicfaolrssiigznes adnudripnugppavritaubriliittiyonatanbdithl,engPthupofobgseestravtaitoino,nsanddurtihnegn tehaec2h1-ldiatye.r ulancftoaltdiionng,peeryieodopienncilnugd.edacopuhsytsiiccalsasrigens,resbpoodnyseweaingdhtasi,t rniugrhstiinngg rbeefhlaevxi.orP,upsiulrfcaocnestrriicgthitoinngwaresfleevx,alpuiantnead pounplsy. onThleactraetmioanindianyg 21p.upsOnwelraectastaicornifdiacyed4,anlidternsecwreorpseierda.ndoTmhley cFuOllemdatloe frouarsmwaelreeansdacrfiofuircefdemaanlde pnreocsrtotpes.ieadnadfesremtihnealenvdesoifcldeosswinegreatwethieghteidm.eofEpvaarltuuartiitoinosno(facstpteiromnnduamybe1r)., mTohrielitteys,teas,ndpimdoirdpyhmoildoegsy, swaecrreifincoedionnclGudDed1i0natnhde npercortoopcsoil.ed.ThPereFgOnagnecnyersattaitouns wfaemsalceosnftihramtedd,eltihveeroevdarbiyesCawcesraereeaxna-msiencteidonforwetrhee fourmbviearbilaitnyd. diTshtreibFuOtigoneonefrcaotripoonrfaemlautleeas, tihmaptladnetlaitvieornedsintaetsurwaelrleywdeerteermsianceridf,icaenddoenmblrcytoastownerdeayex(aLmDi)n2e1d wanads rneeccorrodpesdi.ed.ThOevlarvieersfrwoemreeaecxhampairneendtalasraatbowvaes raenmdovtehde,nuwmeibgehreadnadnddisatnrailbyuzteido.n oBfloimopdlasnatmaptlieosn wsietrees cToilvlresctferdomfrtohme p$umpaslferroamtsthtehaltitheardsomfattheedseanfdivefrdoamms wfeermaelaelsroatcsoollnecLtDed2f1orfaonralpyhsaisr.macokinetic alysis; EGLeneration: tShienc0e.1FaIngde0n.e4ramtei/okngp/udpayvidaobisleitgyrwouapsssiwgneirfeiccanatlry dreidnutocethdeisnetchoend1.g6enaenrdat3i.o2n,mgT/wkegn/tdya-yfidvoeseFIgrgoeunpesr,aotinolny batesgipenrniseonxngpLerDd2o2seangdrcoounptiwneuriengatdhmrionuigshtetrheeddPayFObeSfobrye sgaacrviafgicee.atAdtos2e4sdoayfso0,f0a.g1e,,aonnde0r.at4 pmegrksge/xdpaeyr floirttthienacgaechofdvoasgeingarlouppatweanscytaesntdedoninaLDpa3s4s,ivmealaevroaitdsawnecerepaervaadliugamt.edOfnorLthDe a28g,efoefmaprleepsutwiearleseepvaarlautiaotne.d wOintehirnateapcehr dseoxsepegrroluitptebrewgearneoenvaLlDuat9e0d. iFneamawlateesr-wfiitlhleedviMdoemnaczeeofonmaLtiDng70w.erAessciognnmseindtetor0bceoedhaabtitGaDtio0n `and assigned to individual housing for the remainder of the dosing period. The FI generation male rats " ENVAMRD(2002)17FINAL wfeemraelseascrwiefriecedalalftoewredmattoindge,linveecrronaptsuirealdlay.nd DevaamlsuattheadtadsedleisvcerriebdeldtinrstheweFrOegseancerriaftiicoend.aAnldlnFeIcrgoepnseireadtiaonn dLeDscr2i1.bed AflolrtFhe2 Fg1engeernaetriaotniopnuppusps.were sacrificed, necropsied, and examined on LD 21 as previously 0Tn tchfefcFtOsgeonnermaattiionngmoarleonraatsn,ytohfrethweefreertinloittyrepaatrmaemnetterreslaetveadlculaitneidcalinsaignnysodfostoexigcriotuyp, nteostemdo.rtalRietpyo,ratnedd e1f.f6ecatnsdin3.c2lumdged/krge/dduacytidoonseinbgortouhpbsodduyriwnegigthhet gparien-smaatnidnginpearbisodo.lutFaonldlorweilnagtivmeatfionogd.cfoonosdumcpotnisounmaptitohne swiagsnifsiicgannitfliycanrteldyucreedduicnedthien the 1.6 a0n.d4 3a.2 nd m1g./6kgm/gd/akyg/ddoasyedgorsoeupgsr.oupSsi.gniTfeircmaintnarledbuocdtiyonwseiignhttshewearbseolaustoe m`woeiogthhtesroofrgthaensweemiignhatlcvheasincgleess w(ewirtehrfelpuoirdt)eda.ndAthseigpnriofsitcaatnetwienrcereoabseseirnvetdheinnuFmObemraloefsmaatl3.e2 s wmigth/dbraoyw:n Tver at 3.2 mykgd/osdegraouyp was also reported. fIonrthCeacFsOargceanne-rsaetcitoinonfienmga,lteherartes,dindo ndoetatahpspweearre10rebpeoratendyaetfafneytsdoosneelsetvreol.us Icnycdlaimngs,smaacrtiifnigceadnodnfGerDtili1t0y epamrbarmyeotse.rs,Thtehe onnulymbfeirnsdinogfscroerppoorrtaedluitneathaendFOimdpalmasntoactcounrsr,edorinitnhet0he.4n,um1b.e6,raonfd v3i.a2blmego/rkgn/odna-yvidaobslee rweoiugphst aanndd ibnocdlyudewdeilgohctalgiazeidn aalnodpecfioaodducroinnsgupmrpet-imoantivnagl,ugeesstoabtsieonr,veadnddulraictnagtitohne; apnrde-rmeadtuicntgiopnesriiondboadnyd continuing throughout gestation and lactation Rabeivleirtysi1b0lesudreflaacyesriinghrtefwlaexsasnidgnpifhiycsainctallyddeevlealyeodpmienntthewe1r.e6 oabnsder3v.e2dmign/ktghe/dFaIy gdeonseeragtrioounposffopnriLnDg.s 3T-h1e0 dreemlaaiynsiningtfhoer3f.u2rtmhge/rkogb/sedravyatdioosn)eg.roBuypwtehereenodbsoefrtvheedoobnseLrvDat1ioanftpeerriwohdi,chhotwherveerw,eralel nsoursvuirvviinvginpguppsupsin. ttohesu1r.f6amcge/rkigg/hdtaiynddoossee ggrroouuppsh<ad0.t4hemagb/iklgit/ydaoy.suSrifmaiclearrigrhte.spoTnhseersewweerreenseoednefloayspionbaseurnvfeodldiinnthg anadbileiytey doopesneinggr.oupsA,ltbhyoutghhe etnhedreofwtehreeotbrsaenrsviaetntiodneplearyisodsereenspwointshesthiensepuspisgnwseorfe pshiymsiilcaarltodecvonetlroolpsm.entThaecrtoismseaollf d1e:6vemlao/pkmge/ndtaoyftdhoeseacgoruosutpi.c sNaoreefrfeefcltesxoanndthtehseeabrielfilteyxe(s0aiwerrighotbsweerrveedboitnhtshiegnilfoiwcandtolsyeregdruocuepdoifn 0t.h1e mmggk/gd/daayy garnodup.onlyAtathteranesnidenotfdlealcatyati(oonn(LLDD 2116),onallly)livien ptuhpesabniliatlyl dtoosaeirgrroiguhpts w(0a,s0.s1e,n0.4i,n atnhed 01..46 mg/kg/day) hadpupil constriction response. "dTohseemgsrotupssi.gniTfhiceanrtedfuicntdiinognsreinpoprtuepd viinabtihleitoyffbsepgrainngtwo aaspptehaaroofnreLdDuc4edpopsutpcuvlilanbigliitny tahteth1e.6tmwgo/hkigg/hdeasty oofstehegrpouupps,wweirteh fovoeurnd2d6e%aodfotnheLpDu:psnooupnudpsdesaurdvbiveetdwebeenyoLnDd2L-4D. 1.In Stthaeti3s.t2icmalgl/yksgi/gdnaifyicdaonsteignrcoreuapse4s5i%n tihnedenxumwbaesrgroefatdlaymrsedwuictehdsitniltlbhoersne pduopsse wgerrouepasls(o0o%bsater3v.e2dmayt/k3g.2/dmayy/kaen/dda6y.6%Aasta1.6remsyuklg,/tdhaey)v.iabiTlihtey Jwaacstastiiognnifiincdaenxtwlaysreadluscoedsiignitfhiecahnitglhy-rdeodsuecgerdo(u9p4.a6n%d)tihnertehea1l.so6wdaossae gsriogunpi.ficIanntadrdeidtuicotni,ongeisntatthieonnulemnbgetrh oinf ipmupplabnotdaytiowneisgihttessfwoelrleowaeldsobyobasceornvceodmaittatnhet rtewdouchtiigohnesitn dIotseer sgirzoeu.ps.StatOitshtiecralaldyvseirgsneifisciagnntsriendutchteio3n.s2 mpugps/dhaaty wdeorsee lneovtelnausrssoicnigatoerd wwihtoh rheadductnioonesviidnepnucpe voifabimliiltky ainndtmhaetestmoamlaccha,reaisncwleuldledssltmrastewmmiatlh `cannibalization ofpups that were stlborn or found dead " ENVIMRD(2002)17FINAL tShienc0e.1Fa1ngde0n.e4ramtgio/nkgp/udpayvidaobisleitgyrwouapssswiegnrieficcaanrtelydrietdouctehde isnetchoend1.6 gaennerdati3o.n2.mg/kg/day dose groups, only wCleirneicraelpoorbtseedrvaantdionnos sitnattihsetiFcaIllgyesniegrnaitfiiocanntmdliefferratesncaepspweaerreedreupnorretmeadrfkoarbalen.yofNothtrefaotlmleonwtirneglpaatreadmdeetaetrhss breotdeyntiwoen,iglhoinsg-btoedrym rweetiegnhttiongaoirnsr,esapvoenrsaegienhdibaiytioonf apsreepvuatliuaaltesdepbayrapteiornf;orvmaalnuceesinfoar plaesasmiivnega,vosihdoarnt-cteeorrm wrealtaetirvmeawzeeigphetrsfofrormatnhceeripgahtraodrilgemf; tmesattiis,ngseomrinfaeltvleistiyclpeasr,armiegthetresp;idniedcyrmoissc,opoircpreoxsatmaitne;atainodnst;erambisnoallutbeodoyr pweoisgthwtes.aningThdeayson1l-y8 orcecpuorrrtiendg aetftfheect0s.1wearned 0s.i4gnmigf/ickagn/tdaryedduocsteiolnesvelsi.n absolute food consumption on Cmlaiknigc/adlaoybsdeorsveatgiroonupfoirntchleudFe1d,greendeurcattiioonnsfienmabloedsywweerieghltiskeowinsedauynre| mpaorsktawbelea.ninOgb,sesirgvnaitfiicoannstatlotshsees0.i4n bduordiyngweliagcthattioonn. LDTsher1e4,wearned sniognisfaitciasnitcarleldyucstiigonnisficinantfodoidffceornesncuemsptrieopnorotneddafoyrs a1n-y8 opofstthweeafnoilnlgowainndg peavraalmueatteerds:by vpaelrufeosrmfaonrceleairnniangp,assihvoret-atveorimdarnecteentoiron,waltoenrg-mtaezrme preetrefnotrimonanocre rpeasrpaodnisgem;inmhaitbiitnigonanads fertility parameters; gestation index; pregnancy rates; and necroscopic examinations. wEeviigdhetnsce(oofantpreeratmietntrerbealsast)edobefsfeercvtesdinatth.e Fm2g/gkenge/rdaatyioonnpLups4Dcaonndsis7.tedBoodfyrewdeuicgthitosnweinremecoamnpapruapbbleodtyo wcoenrteolobsleevrevlesdboynLLDDs14.7 aAntd01.44. mgM/ekagn/dabyo,dystawteiisgthictaslloynsiLgnDi2f1icacnotntriednuucetdiotnosreinmamienalnopwuepr tbhoadnycownetirgolhst,s caolntthroolusg)h. thCelidniifcfaelraenncde nweacsronsoctopsiactiostbiscaellryvsaigtnoiiffoitcnahnset F(246.g5eneraitni0o.n4pmugp/skwge/rdaeyundroesmeargkraobulpe.vs.NoSo0tgheirn onicologically significant effects were reported pUanrdeenrtstaherec0o.n1dimtyiokngso/dfatyheansdtu0d.4y mthgekeN/OdaAyE,LreasnpdectLiOveAlyE,LbafsoredbootnhrtehdeucFtOiognesneirnabtioodnymwaelieghatndgaifnemaanlde firoeoadtmceonntsruemlpattieodn.reduTchteionNsOiAn EabLsolfuotretfheooFdIcognesnuermapttiioonn pvaarleunetsalwemraelersepocrotueldd antotthebeloewsetsatbldiossheedtessitnecde, 00..14 mmigg/kgd/adayy,. reTshpeectNiOveAlyE,LbaasneddoLnOAsiEgnLifficoarntthereFdIuctgieonnesraitniobnodpayrewnetiaglhtfsemaanldesfoaorde 0c.o1nsmugm/ptkigo/nd.ay Tahned bNaOseAdELonasnidgnLifOicAaEntLrfeodructthieonFsI ignentehreatniuomnboefrfsopfriinmgaplraen0ta.t4iomng/siktge/s,dalyearndsi1z.e6, mpgu/pkgv/idaabyi,lyr,esppeucptibveoldyy, mwegi/gkhgt/daanyd, sruersvpievcatli.velTyh,ebaNsOedAEonLsiagnndifLicOanAtErLedufcotritohnesFin2mgeenaenraptuiponboodfyfwseipghartrie0n.g1 mg/day and 0.4 Lfoirvemralaensd asenrdaosnamlpalcetsaownerdaeyco2l1lefcotredfefmraolmest)heaFndOaannadlyFz1edanfiormatlhse aptretseernmcineaolfsaPcRriOfSice((3aMfteErnvciorhaobnimteanttiaoln Laboratory, 1999a). The resus for the FO animals are presented below: 4s ENVAMRD(2002)17FINAL (Dmogskegglrdoauyp) AvienrsaeguemP(FuOgSmlc)one. Avienrlaivgeer P(gFlOeS)cone. 00 fmeamlael0e.00.2043407 mfaelmael0e.06.61571 01 female 528 female 14.3 male 105 male 84.9 04 female 189 female 58.0 male 45.4 male 176. 16 mfaemlaele15822 mfeamlael3e23184 52 mfaemlael2e7N3R* "samples not received femamlaele13N6R0* Qaunadliltivaeirvse.lyP,FtOhSe cFoOncreensutlrtastiionndiicnactreeaasledatwsit(hindcosleu.dinPgFcOonStrcoolnsc)enhtardatdieotnesctwaebrlee hliegvehlesroinftPhFeOlSiveirntsheanruimn tfheemasleersoumf,tahned smaamleesdhoasde ggrreoautpl.y iPnocorleeadsedlivPerOsSamcpolnecsenftrroamtitohnes iFnIsaenriummalasndsaclriviefricwedhesnhocrtolmypaarfeerd bwiirtthh hPaFdOSlocwoenrcePnFtrOaSticoonnsceinntproaotlieodnslitvhearnsaadmuplltessoffrtohme FFIO agneinmearlastsihonorolfyatfhetesrabmiethdowseeregr0o.up0.811,Th6e.19a,ve5r7a.g6e, afonrdt7h0e.4PuFgOlSg icnontcheen0t.r0a,t0i.o1n, 0in4,thaendli1v.6emrga/nkdgs/edrauymdsohsoeulgdrobupes,vireeswpeedctiwvietlhy.caTuhtieosne. quTanhteitaatcicvueravcayluoesf uqunacnotrirteacttieodndsilu=ti3on0%e,rotshe.purity of the analytical reference substance is unknown, and there were several nTewonoatset.udiIenstwheerferstthsetnudcyo,nSdpucrtaegduet-oDafwurltehyerrautnsdewresrteanaddmtihneisdtiesrterdibourtailondoosfesPFofOSPFiOnSth(e0.1d,am0,4,fe1t.u6s, aanndd o3.2f pmrue/skugm/eddayg)esotnacteiodnai(l3yMbeEgnivninrionngm4e2ntdaalysLapbroiroatoorcyo,ha2b0i0t1aet)i.on,Saenrudmc,onutriinneu,edantdhrofeucgehsdsepyec1i4omerndsaywe2r0e cGoDl2l1e.ctedAftrootamltohfe5d4amposobleefdosreermuamtianngdalnidveratsGpeDci7m,enGsDw1eracnodllGecDt2e1d;frloimverfestpuseecsimoennGsDwe2r1e. coTlhleecrteesdulotns are shown below: Average Results for the Analysisof Serum Samples (ug/ml) Omgke Olmgkg O4mgky lmgke 32meke DaDayyo7 000.712363 878892 40079 1is640 s1i0s5 DaDayy 2115 o00n92s6 a8820 2a6n2s 115366 2i7sss Day2i Fel 0.125 9.07 343 101 165 a ENVIMRD(2002)1 7FINAL Average Resuls for the Analysisof iver Samples (DmogskeeGirdoauyp) PFFOeSmaColneeA.du(lugt/g) ~~ PFOFSeCaonLci.ve(rug/g) 001 022828 0719639 0146 314077 336076 32 610 20 Itnhegefneetrusaels,.hePrReOwSawsaasdaolsseo-roeblsaetredveidncirneatshee cinontthreolledvaelmss,ofasPwOelSl iantthheeicvoenrtraolndfesteusreusm. oAfsthoebdsearmvsedanidn ltihveer2-tgheannerianttihoensreerpuromdfuoctitvheetdoaxmisc."ityTshteudly deesvcorfiebPeFldOasSbovree,mahieneldevfelisloyfsPteFaOdSy iwnertheemsuecrhumhiogfhtehreidnatmhse wfraosmgGenDerOa-llyGIcDom1pSa,rbaublehe0 ltevheelsledvrelopopbesderavteGdDi2n1t.heIdnatmhse,GwDh2e1reafestutshees,letvheel loefvePl FoOfSPFinOStheinftehtael sleivreurms was wel below that seen in th dams. oTnnctehedasielcyonbdegsitnundiyn,gS4p3radgauyes-pDraiworletyo rcaothsabwietraetiaodnmuinntiisltceornefdiorrmaelddoevsiedseonfcePoFfOmSat(0i.n1ga(n3dM1E.6nvmigrlokngm/ednatya)l, `Lnabeordaatyorpyr,io2r0t0o10in.itiUartiionnoe fancdohfaebciatlastionattmhweeprfeocllolloleweicntsgedmofmrionmgt,hedadyasm6s 1d0u7r,ing14t.h1e0 f1o5l,laonwdin2g0inttoer2v1also:f pthreesduamyecdohgaebsittaattiioonnawndasdainyisti2at1e-d22(porfiolract1a0ticoonh.abiBtlatoioodn)s,aGmpDle7s, w1e5raencdol2l1e,ctaenddfLrDom1c4acahnodf22t.heDdaayms1 oonf plaucptsatpiorn lwiatser,dewfhienreedpaossstihbeled.aySoefrbaisrtphe.cimOennsLwDer4e, cloitltleercstweedrferocumlpleodolteodflivtermaslaemppluepssonanLdDfi2v1e. feLmiavleer Bspleocoidmseanmspwleersewcoelrleeccotleldecfterdomantdhepodoalmesd,paenrdltthre.lTvhee frreosmuseaacrhe psuhpowwnasbelcoolwl:ected and pooied per liter. Average Results for the AnalysisofSerum Samples (ug/ml) Omgke Olmgke 16mekg aap7o 000.19006 972214 112619 GGD21is 0<.L07L4Q2 62588 95056 bi2n2 00005022 0196730 2i0a5l6 (D3 pups 00531 150 2 Average Results for the AnalysisofLiver Samples (ug/g) Omgke Olmgke Lomgke DPaump 002183m 6s1o5n 556927 tIhnegleenveerlasl,inthtehreeswearsumaddeocsree-raesleadtewditihnctriemaes.e TinhethelevleelveslosfoPfRPOFSOSweirnetmheuclhivehriagnhedrsienrtuhmeofivethretdhaanmsinatnhde o ENVIMRDQOG)I FINAL Steerluemvoeflsthinetdhaesmesraunmdwtherpuhpis.ghlTyhehilgehveerlsinofthPeFpOuSpswtehraen sinihleadnast.he fiveof the dams and pups, while uBapsevdiaobinlitthyewreerseulosbsoefrvheed attwo1.6-agenndera3t.2onmrepgrodu/ctaivdcertooass-cFiyotsytesr,tiundgysitnudwyhiwcahs csiognndiufcictaendt3r5eadumcteiaonnss oinf otfeerxmpiosnuirneg dwuhertihnerlctihtoenff:ectthsuosbtsheervpoetdenitnipalupfsoWredristirnecstuilotnotfo ibnewmaidrebexeptowseuerentporePnaFtOaSl aondrpaosreisutl effets Tllowing coninuous matemal weament (Chistian etal. 199) iInn t0h.is5%dyTw,eewno-$g0robuypsgoafva2g5e,febmeaglienSnpirnaggu4e2Dadavyseypriraotrs twoermeataidnmgini(s0teurnerda0teadnd(1b.r6eemdgerr)kmgadlyes,PFaOnSd cadojnutsitneudindgaitlhyroounghtohuet gbaessitsatoiofnianndidvtidoualdabyo2d1yowfeiagchtta.tionP.areAndtoalsefevmosliuemsewoerfmobLskergvewdastwaidcmeindiasltyerefdo,r paebriilodi.tyMaandtrclainibcoaldywobesiegrhvtatsiwoenrsewreerceorrdeecdorodnecde 1drhoiunr tphreioarcctloimaantdioanfpereidoodsianngd dtuhreinndgaitlhyedturreiantgmetnhte arndetahemn dpaeirliyoddaarnidngatgessatcartiifiocne:anfdooodncdoanyssum1,pi4,on7,w1a0s, salndo e14coorfdeladctoatnioen.durDiunrginhgepaarctculriimtaiotni,onfepmearlieosd Swiezrees.caonndtipnuuapllvyiaebivlailtuyatatedbifeor.lnMiactalersaignbsehaanvdiolrswafsorredcuorradtieodndoaflygestthartoiuognh,oluetngltachtaotfiopna.rtuArliltimoant,elrintaelr ttahtosrawciecr.e aSbadcormiifnicaeld, byancdarpbeolnvidciovxiisdceeraaspwhaysxiapteirofnoromnedd:yan2y2 roofsasctleisoinonsanwderaegrporsessenrevcerdopfsoyr offuttuhree lainavleysris. TIinteardwdirtieonn,othienmcbheedsianhdedcriosss.fsosotrionfpglparnotcaedtuiroenansdeswewreersacrreicfoidceedd.on Rlaatcsathoant ddaiyd n2o5x. eDxaamsinweidthfonrogrsousrsvilveisnigonsp,upasndwtehreeustacrriefixcaemdinafetdr0thceolsntfputphepwraessefnocuendabdseeandc.e moifssiimnpgl,anotratpiroensiucmse.d comnibalized Fploalcleodwiwnighcocmiptlehtriocnonotfolp.artourriPtFiOonS,-tlrietsertsedwedraem ifmomrerdeiaaritnegl.y rTheimsovcerodssf.froosmtertihnegi prreoscpeecdtuirveerdesaumlstedanidn iTnoutregrryouepxspoosfur12e-o13lyd:amBs) ocronpturopls dasamfsollwoiwtsh: lAi)tercsonftrroolmdcaomnstowlitdhanist,eis..r.onmegaPtFiOvSe-croenatrtoeld: da)msP,FOiS.... wPeRaOtSe.d dcaatmesd wdiatmhslwiittehrstferrosmfPrFomOSc-otnteoaltddadmsa,msi,c. i.p.o.stb-ontahtalinxpwaesruoraenodlyp.ostnatal exposure; and D) dOunridnagyc1aotFioancafioronvi(abbiirltyh)., cPaucphspwueprewaosbsinrdvievdiduoanlcleydwaeliyghfeodr calnidniceaalchsilgnesranwdasgreovsaslueaxtteedmatlwipcheysdiaclayl Oannomadlaiyes4. ofPulpacbtaotdiyon,weeiagchhtscwroesrse.rfoescteorreddeloindterdawyass cu1,l4,le7,d 104, nmda2l1eosfncdat$iofne,malaensd. thOennatdasaycr2i1ficoef Isaaccraitfiiocne,dalblecpauupsse woefremesraicbruifnidcietdyviwaerdeeceapxiatmaitinoendafnodr erxoasmsinleedsiofnosr ranodsfolresitohnes.cauPsuepsoffoduenadthdeoardtohre tmiomreipbouinntdscoanndidtiponr.eseTrhveedlufnogrs,piosesri,blaendfauney graonsaslysliessionsLwieverre csoalmlpelcteesd fwreorme seevlaelcutaedtepdupvsiaatcvalrioouns mSiaclruosscwoepry:e usTehdewmheetrheoadopfprsaotpsrtiical analysis consistedofcalculation of averages and percentages: ler sTnelaecdtdedinma,tesrammapllerastsoafnbdlopoudp,sm(ibllkooidncalnuddiinvgertshammpilleksswcecrreinpogolgeldanpde)r, taenrd)laitvervawreiorues ctoilmleepcotiendtsfofomr analysis ofPROS concentration. Only the analysis ofhe sea samplesar aailable at his te. AStudmyaptecrmiaold rea2ts.sucrhvriovmerdhti0nsocrheehdeual,essccaliyftil., aSbirganssioofncloinnitchaelheoaxd,iinyecko,bstearlveanddilnorthfeodrealmismb,dumriisnsgintgh.e = ENVAMRD(2002)17FINAL trberaotkmeennta.nrdelloartemdissianlciegntehdeyianlcsiosoorcsc,urarnedd lionctahleizceodntarloolpeacniiam,alsa.moMneganothmeartse)rnwaelrbeodnyotweciognhstidgeirends a1t0 1b.e6 mMega/ndaatyemwaelrebordeyduwceeidghctsomapnadrebdodyto wceoingthrtolcshadnugreisnginptrehmattriantgedagnrdoucponwteirneuicnogmptahrraobuglheotuot tgheossteatsieoenn cinoncsonutmrpotliaonniwmaalssrdeudruicnegdlaicntattrieoant.edDaunriimnaglsthaespcroemmpaatriendgtpoecroinotdr,oalsn.d onRediuncotgieosntsatiinongeasntadtiloacntalteinognt,h,fotohde `Aalverpargeegnnaunmtbaenriomfalismpdlealnitvaerteidonivsieteosf,fdsperliinvge.red sizes, and live liter size were observed in treated animals PFoulplmoowritnaglictryowsass-ofbossetrrvieondnginL(Dwoo1,ftlihvee lcirtotesrs-sfiozsetserweedregrcouopmsp.arOabnlelacbteattiwoenendatyrsa2t-e4d,aanppdrocoxnitmraotlelgyrou1p9s.% oifntghreouppupsAiantgarorautCepowfe9r%e.eithIenrafdodiutnidond, oneordparyaes4udomfedlacctaantniiobna,litzheed.nuPmubpermoortfalliitvey pwuapss,alnsoumobbesrersveodf `srurovuipvsinBg apnudpsDpedurrilintgerl,acatnadtiolnivedalyiste2r-4siwzeesrewearte1a.l6s%o arneddu1c.e1d%,inretshpeescetivtewloy.groRuepdsu.ctiPounps imnorpaulpitbyodiyn `wceoimgphatrsabwleeretoocbosnetrrovlesddiunrignrgoutphast AsamanedpeCrioodn. daFyro1molfacltaacttiaotniodn.ay 4P-u2p1,bpoudpybwoediyghwtesigihntsgrionupgroDupwserA.e Can"dTaeDTwieterresriendgucerdoAwhuaennpdcoomnpearleedr0ongrgouprBCo(dneiugdantpiovte cnuornst.rol)Miwlikthantahleysriesduocfttihonessgrteaotesmtoainfcgprhuopsusp Af.ouCn.dannodmDi,lkreisnpetchteivseltyo.macShesxorfat$io7s%a,nd10t0he%,laacntadti8o7n %inodfextwheerpeucposmfpoaurnadbldeeaamdoanndganlecrgorposuipesd. Siniggrnosuopfs occlicnuircraledtoixnigcityrwBeor(eneougbastepirvveedconitnroplu)psa,t tbhuetswaemreernaott. considered (0 be treatmentrelated since they also. Eflreocmtrdoanmmsictrreoastceodpwiictehxa1.m6inmagt/ikogno/dfatyhePFOiSv.e rNeovesailgendiafnicainntcrdeiafsfeeriennctehsewneurmeboebrseorfvpeedrobxeitswoemeensgirnoupuppsB. and the other groups following examinationofpup lungs. 1P0F5O.S34cognmce.ntraSteironusm iPnRthOSsceornucmeontfruanttiroenastiendtdheampsuprsanfgreodm furntormeabteeldowdatmhse,lfimoistteorfdedetweictthiuonnie(c0a.t0e5dudga/mls). wwietrhe ubnetlroeawtetdhedaimmsi,t roafngdeetdecftiroonm. 47S.e6ruugm/mPlF0OS59.c2oncuegn/tmrla.tiPonRsOSinctohnecepnutprsatfiroonsm itnretahteedsedraumms,offotsrteearteedd fdoasmtsererdanwgietdhfrtormeat5e9d.2duagm/sm,l r1a0n1g5e7dufg/rmolm. bSeelorwumtPheFOlSimictonocfentdretaetcitoinosnin10the35p.u7psugfiruol.m unStreerautmed PdaOmsS, gcoinlc.entrTahteisonesdiantthiendpiucpatseftrhoatmetxrpaotseudredatmosP,FfOoSstecraend owcictuhrrbeotehdinduamesr,oraanndgevdiafmriolmk7f9r.o5mutgr/emalted10d9a6m.s9 (3M Environmental Laboratory, 19995). The accuracyofquantitationfotheanalyseswas + 30%. einxpcoosnucrleusoinolny.) phuapdsthferosmamceontlroowl mdoartmaslitthyatratweer(e1.c1r%o)ssa-sfopsueprsedfrwoimthcoPntFrOoSl-rdeaamtsedcrodsa-mfsost(eproesdtnawtiatlh cdoanmtsolwidtahmlsite(r1s.6f%r;omnePgFaOtSiv-erecaontterodl)d.amMso,rtia.l.i.ty ruatteersoienxtphoesurreemaoinnliy:ngantdwoPFgrOoSu-pts,rehaoewdevdearm(siw.ithcolnitteorls f1r9o.m2%P,FrOeSs-ptecrteiavteleyd. daUmnsd,eri.teh, bloimtihteidncuotnedriotiaonnsdofpotshte-nasttauldy,extphoesudraet)a,awepreptmoueicnhdaicharitgehetrhatatre9d.6uc%ed paunpd Scuornvjiuvnacltiiosn mwaiitnhlyin autreersoultexpoofsuinreumtearyo aelxspooscounrteritboutPeFtOoSreaduncdedthaptuppossurtvniavtaal.l exInpocsounrtreasvti,aexmpiolskurien dviuarbiilnigtyl.actAadtdiiotniaolnoanlel,y,tharnoaluygshismiolfkPfFrOomSecxopnocsenetdradtaimosn,sdhooewsendotthaaptpePaRrOtSo hwaavseoabnsyeravdevderisnetahfefescetraonofpuFpO. Sfaemmpalleesratsakeenxpofsreodm dFu1rignegnetrhaetiionn-lipfeuppshafsreomofftehmealsetudrya.ts eAxdpdoistieodna1lly,thePFtOesSt wsuabsstoanbcsee,rvaenddininseFrIa eneraton pups exposed via lactation, but not exposed in utero. w ENVIMRD(2002)17FINAL PServeelriamlinmaercyhraensiusltsicinsdtiucdaiteestahraet rbeediuncgticoonnsduinctseedrutmo ulnipdiedrssatnadndchtohleesnteeornoaltaslyndtehaetshis(d3oMnoCtomapppaenayr,to20l0a1)ya, significant roe in the death ofthe offspring. 38 Human Hazard Scoenvdeurcatledo.ccuTphaetiofnoalllowsitnugdisetsudoinesthhaeveheaallltbheeefnfeccotnsduacsstoecdiaattetdhewi3tMh Dfelcuartourc,heAmliacbalameaxppolasnutrewhhearveePbFeOeSn tHhaes Abnetewnemrapn,ufBaecltguiruemdpslianntc.e 1O9n9l1,y asnodmePFoOfAthesisntcuedi1e9s98e.xaTmihneesdtuadipeosssciobnldeucastseodciianti2o0n00beatlwseoeinncsleurduemd PsuFrOroSgalteevselfosrliunowroorckheermsicanadl csoerrruemsploenvedlisnginhweoarlktehrse.ffects. The other studies use exposure categories as Cwreorses-saencytioansaslocdiaattaiofnrsombmeetdweieanl sPurFvOeSillalnecveelsconadnudctehdemaatttohleogpyl,antcsliwneircealancahleymziestdrtioesd,etaenrdminheiorfmtohnearle p1a9r9a5meatnedrs1(9O9l7seannedtavlo,lu1n9t9e8e,rs19f9r9o,m20t0h1e2)2.00A0 gmreodiucpaolfsmuarlveeilvloalnucnet,eewrhsiwcohrkailnsog aintchleudDeedcafteumralpelsantanidn Antwerp employees, were analyzed. aInndthethe199h5emaantdol1o9g9y7 aannadlysoetsh,erthcelrieniwcealrecnhoecmoinsstirsytepnatraamsestoceirastiwohnsenbettwheeendatPaFOfSrolmeveblosthin ptlhaentwsorwkeerres cloevmeblisnedde.penMdienagnosnertuhem ycehaorl;eshtoewroelvelre,vetlhsosreemeamipnleodyceoensstwaintthotrheinhcirgehaessetdPwFitOhSilnecvreelassihnagdPlFoOwSersmeeraunm tHhDerLe wvaelrueens.oaIstssohcoiualtdiobnesnboettweetehnatclaigneicwalashespiagtniicfiecnazntylmyeastseostcsiaatneddsweirthumhiPgRheOrSPlFeOveSlsleinvewlos.rkeInrsa.ddition. TElheeveenmphlooryemeosnewhvaolupeasrtwiceirpeatceodliencttehde hforrommo8n8e opfortthieoneomfptlhoeyesetsudwyhwoerpearstiigcniipfaitceadntilnytdhieffsetruednty finro1m99t5h,e. aodnj-upsatritnigcifpoarntcsonaftoubnodtehrsl,ocatthieornes w(eyroeungneor,sihgingifhiecrantPFaOsSsoclieavteilosn,ssmboektewrese,n aPnFdOhSigahenrd WtBhCe)h.ormAofnteesr panpaml)yzeadn,d eaxclearpgteeBstMrIadimola;yhhoawveeveirn,flituesneceedmsthtehsaet roensueltesm.plRoeymeoevawlitohfhtihgihs ePmFpOlSoymeeeasfurroemmtehnetsana(l1y2s.e5s3 resulted in no significant associations with estradiol. cTohnesruemwpteiroens,evaenrdaldagief.ferIenncaedsdbietitowne,etnhetrheeweemrpeloayneleys6a1t tehmepltowyoepelsancto,msmuochnaos bboodtyh mtahses1i9n9d5exa,nadlc1o9h9o7l ccoonhsoirsttednuteovoerathheig2hirmaeteopefriotdus.moTvheeartparbtaitchippaltaionntsraftreoimn t1h9i9s6-v1o9l9u7n.tarySboimoemonoiftotrheinfgiwndaisngvserwyerleownoatt PthFeODAecsaetruurmpllaenvtel(s3i5n-4109%9)5. weIrnead1d.i4t6iopnp,mthaenedmipnlo1y9e9e7swaetrebot1h.57sitpepsm)u.sedThAePnFuOmbaesraosfuyrefacrtsantthat(mceaacnh germopulpoyeweosrkheadd tathetlhoewe2stpllaenvetlss(oefxpsoesruurme)PFwOeSr.e nAoltsop,rnoivniedteyd-fiivne tpheercaennatlyosfesth,eaelmtphloouygehesthaecryoosusngbeostth rpleatssuoalfntdthibssotshtutdiymeshpoeurlidobdsehiandtesrperreutmedPcFarOeSfullleyvels less than 6 ppm. These limitations indicate that the "lTohceatiroenssucsomfbrionmedthaend20b0y0pclraonsts-ssienccteiotnhaelAanntawleysrips awnedreDeacnaatluyrzpeodpufloartiaolnl sewmeprleoyseiegsniffircaonmtlbyodtihfeprleanntt Sferroummcalcehvelosth(emreiannse0v.e9r6alpwpamy)s.. weArnetwyeorupngeemrp,lohyaedelsoawsercoBmMpSar,edwotrokDeedcafteuwreermypelaory,eehsadhahdiglhoewreralPcFohOoSl consumption, higher mean HDL and bilirubin values, lower mean triglyceride, alkaline phosphatase, GGT. s0 ENVAMRD(2002)17FINAL bAySTp,roadnudctAiLoTn savtaluusesa,ndangdenmdeearnatnhdyrdoiivdidheodrimnotnoeqvualauteseeofntdhtereobsdeeruhmighPerO. SThdiestdraitbuatwieonr.eaHloswoesvterart,iftiheed PROS leavredeiffleresntforcach subgroup, making it difficult to compare th results. Chomepamriasotnsooraulcrrioonsasgnaqlyuyasr,itsilevsaluweis.thTinheploannltylosciagtniiofniscandtid(p n<ot05r)evdeiaflfermeannceys dnioftfeedrewnecrees: inBUthNyrowiads, spliagnnti,faicnadntAlyLhTigwhaesr isnigmnaifliecapnrtoldyuhcitgihoenrwionrtkheerhsigahnedstfequmaarlteisleininthDeechaitguhresmtaPleFOprSodquucarttiiolnesemaptltohyeeeAsn.twerp. `heWphaetnicthcleindiactaal cwheermeisatnraylytzesetds banydnluimvebreernozfymeempalnodyebeilsirwuhbion hteasdt,vaalhuiegshearbopveerctehnetargeeofefrenmcaeleraDnegceatfuorr pparnoedluctthiaonntwheoroktehresr qwuearrteilien.thMeohsitghneosttabPleFOwSerqeuatrhteilreesu(l2t.s3f1o-r1A0L.0T6 wphpemr)ef8o%roAfLeTm,plGoGyTe,esanidn tthoetallolwievsetr rPaRngOeS, ewxhpiolesutrheegpreorucpent(aQg1e)safnod t2o8ta%l liinvetrhepahnieglhe(swthiecxhpoisnucrleudgersoaulpka(l0i4n)e hphaodspvhaaltuaesse,abAoSvTe,thAeLTre,feGrGenTc,e parnoddtuocatlioanndedmiprleocytebeisl,iriunbiDne)wcaetruer 1fe8m%alaens,d o3r5%i,n arensypoefctitvheely.AntTwheisrtpreenmdpwloayseenso.t eHviodweenvteirn,Dietcsahtouurldnobnenoted that each sub-population had different serum PFOS quale disbution, HMiegahnervianluQe4s (fomretarniglPyFceOrSidelse,veallk2a.l6i9n,erpahnogseph1a.t6a9se--,1o0t.a0l6 bpiplmiu)bitnh,ananidn QAILT(mweearnePsiFgOniSfilceavnetlly0.(2p7<pp0m5,) orfanAgnet0w.e0r4p--0pr.o4d2ucptpimon)efmorplalolyemaelseweemrpeleovyeenelsyadtisbtortihbuptleadntasm(onn=g4t2h1e).quaItrtsilehs wohbiuelnelotthdiesdwtahstntohtetnhuemcbaseer: ffoorr eDmepcaltouyreeesmpwlohyoeehsa.d vTahleuehsigahbeosvtenuthmebererfoefreDnecceartaunrgeemfpolroaylekealsiwneasphionsQph4a.taTshe,esAeSdTa,taALwTe,reGaGnaTl,yzaendd tHootwalevleirv,ersptaatnieslt.icalFosrignmiafliecaencmeplwoayseensotforrepaolrteodf. theInseQm1e,a4su%reo,f tthhee leemvpellsoyienecsrehasaeddvfarluoemsQaIbotvoe Qthe rlievfeerrepnacneelr,an1g4e9%foorftAhLeTemapnldo6ye%esforhaGdGTva,luwehsilaebo1v2e%thweasrerfeerpeonrcteedrafonrgeQ4infQo1r baosthcoofmtphaerseedt0ests2.3%ForintQo4ta.l "eTmhpelnouymebsercsoomfbfienmeadlfeo embpolthoypeleasntswi(tnh=va9l7u)e,saalkbaolvienetphehorsepfhearteancseeraanndgeGGwaTswveerrey ssimganlilfi(cnan=t)ly.hiIgnhefrem(apl<e 105) and toal bilirubin significantly lowerinQ4 than in QI. <"T0hy5r)oiadndreTsuHsBRfor(Tm3aul prpodutwcatisaosnigeknmipflieocyaen)telsyolfowbetrh(ppl<an.t0s5)inidnicQa4tedthtanhaitnTQ31.asThsiegnriafnigcaenotflyTh3igvhaelrue(sp wvaalsuevseraycrolasrsgethefoqruafretmilaelse.s; however, thre were no statistically significant diffrences between the mean rMuelstisvoarfitahbleecrleignirceaslsicohnemainsatlryysetsestws.ereThceonfdoulcltoewdin1gvaardijaubstlefsowreproessiinbclleudceodn:fopurnoddeurcstitohantjmobay(yeasfofercntot)h,e plant age, BMI, cigaretes/day, drinksday and years worked at the pant. SAigpnoisfiitciavnet s(ipgni=fi0ca0n0t1()p =in 0t4h)eamsosdoecli.ationBMbe,twceiegnarTe3tieasniddaPy,ROaSlcwoahosliodbasyervweedr.e Pallasnotsliogcnaitfiicoanntw.s Ihnigthhley uHnoiwvearvieart,e faonraleyascehs,plAannttw(eirndpiveimdupallolyy)eeTs3hvaadluheisghienrcremaesaend bTy3qulaervteillse tahsanPFDOeScatsuerruemmpllevoeylesesincorveearsaeldl, waelrheoungoht stihgnidfiifcfaenrtenicnetshewerregerensostiostnatainsatliycsaelsl.y significant. THBR, as well as the other thyroid hormones. AWheponsitbiovteh sPtaFtOisSticaanldlyPsFigOnAifiwcaenrte(ipnc=lud0e4d) iansstohceiamtoidoenl,bentewietheenr wPeFrOeSstaantidstcichaollleysstirgonlifiwcaasntoabtsepr-v.e0d5.. si ENVAMRD(2002)1 FINAL (PpR=OS01w).asNnootssiiggnniiffiiccaannttalsysaoscsioactiioantsedwweirtehoHbsDeLr,vebdutwirtihglPycerFiidensOrwelearSteiopnostiotiavleklayliansespochioastpehdatwaisteh, PGFGOTS, AST or total bilirubin. AAuolrooncgihteumdiicnaallsanoavleyrsitsimoef itherealabtoevdetdoacthaanwgaesspienrfcloirnmiecadl tcohedmeitsetrrmyinaendwhpeithderreosuclctuspaitnieomnapllocyxepeossoufrtehteo A2n0t0w0ewrepraendanDaelcyazteudrufsaicniglitmeusli(vOalrsieanebltealr,eg2re0s0s1ibo)n.. TThheemepldainctaslwseurrveeialnlaalnyczeeddatuasifnrgom3 1s9u9b5c,o1h9o9r7t, tahnadt eimncplluodyeedesthofsreomwhboothparptliacnitpsatweedrien c2omobrinmeodr,enmoedsitctailsiecxalalmyssbigentiwfeiceannt1(9p95<an0d5)20a0s0s.ociaWthioenns wmearlee aosbssoecrivaetdioonvsebrettiwmeeenbePtFweOeSnaPnFdOcShaanngdeschoovleerstteriomleoirntHigDhLy,cearldkeasl.ineIpnhoadsdpihtaitoans,e,thGeGeTw,erAeSTn,o AsiLgTn,ifitcoatnatl bilirubin, and direct bilirubin. PThReOrSe wleevreelsseivnertahlesleimwiotraktieornss htaovtehebe2e0n00deccrloisnsisnegctoivoenralimaen,d 2l)onPgiFtOudSinsaelrsutmudileesvelisncwluedriengs:ign1i)fisceanrtulmy sheivgehrearlactontshiestDeenctatduirffpelraenntcetshainn caltintihcealAcnthweemripstplapnrto,fi3l)esalalnpdardteimciopgarnatsphwiecrsebveotlwuenetneeresm,pl4o)yteheesreofwetrhee 6D)ecpaltauntr paonpdulAanttiownesrpcapnlnaonttsb,e$c)oPmFpaOrAedanbdecoatuhseer qpuearrftliuloersainraeteddicfhfermeinctfaolrseaarce halssuobpgrreosuepn,tainndth7e)soenpllyanosn,e moengaistuurdeimneanltsattudayceorntlayinaposimnatllinntuimmebewrasofcoellmepcltoeydefeosr epaarcthiccilpiantiecdal icnheamlilst3rysatmesp.linIgn apdedriitoidosn,(i2n4%t)h.e ianfaflryeznetd lbaebcsaausned oafnatlhyetiscmaallltenchniuqueomsffpoabrrtPiFceiOpSanrtwse.re used each year, and female employees could not be eAmprleotryoesepsechtaivdesicgonihfoirctanmtolyrtdailfifteyrsetnutddyewatahsepxeprefroiremnceedsofnroDmectahteugreneemrpallopyopeuelsattioonde(tMearnmidneel wahnedthJeorhnpsloann.t a1n99d5)1.991T.heSreevewnetrye-f1o9u5r7deeamtphlsoyweeerse wrehcoorhdaedd,waonrdktehderae wleearsetnoonseigyneiafricaatntthley DeleecvaattuerdpSlaMntRbeftorwealelnma1j96o1r cWahuseesotfhdedaattahwerregearadnlaelsyszeodfbthyejocbomdpeasrcirispotniopno(pcuhlaetmiiocnalusdeedpa(rAtlmeanbtamvas. sftiatlem,pUlSan.toermpAlloayebeasm)a,ctohuenrteiewsa)s.. a statistically significant deficit for all cauosfdeeasth in bothjobcategories. S"Tihnicsestthuedywohrakdfoarlcmeoswtascormepllateitveelfyoyloluonwg-,upoonflyth7e 4cdoehaotrhtsawnedreasrceeprotratiendmfeonrto1f,9c5a1usemepslooyfeedesa.th.TheHonwuevmebre,r oreflawtoiomne0n minortahleitsyt.udy was very small (4 deaths). Additionally, PFOS serum levels were not examined in `Aanddiunpgda7tyeeaorfstohfisfosltuldoyw-wupas(Alreecxeanntdleyr,co2n0d01u)c.tedTh1i0s cfoolhloorwt ctohnesicsotheodrotft2h0r8ou3ghemDpelcoeymeebserwh3o1,ha19d98w,ortkheuds altastthecoDheorcta.tur Ipnlantthifsorstatudlye,astem1pyleoayr.eesThweerreeweprleac1e4d5 idneaoth3s reexppoortseudr.eaicmaotesgtortiweiscebtahstedreopnorttehdeiirn jtohbe deexspcorsiuprtieongsr:ouph,igihn, wlhowi,chantdheneone-rxepos6e5dd.eatAhls.mosFtouhratlefoenfptehreceenmtpolfotyheeesco(h4o7r%t)wwaesreinptlhaeceldowinetxhpeoshuirgeh rou (27 deaths), and 39% were in the non-exposed group (53 deaths). `weSrMeRnowtereelecvaaltceudlaftoerdmuossitngosfattehoe fcaAnlcaerbatmyapersefaenrdenfcoerdantoan.maWlhiegnnanthteceanutsierse.cohSoMrtRwSatshaanawleyrzeeda,bSoMveR.| (coclaonrcterwoafstlhiemietseodpthoagaunsy,elmipvleo,yebreeaesvte,ruerminpalroyyeodrgainnsa, hbilgahddeexrp,osaunrdesjkoibn)(ewxecreeptablrseoasetlecvaantceedr).whOennlyth2e or 3 deaths were reported for cach of these cause-speciic categories and were not statistically significant, 2 ENV/MRDE002)1 FINAL except for bladdercancer t`hWeorgkeenresrawlhpoopwuelraetieomnpolfoyAeldabian mhaig(hSeMxpRos=ur1e2.j7o7b,s9w5e%reC113=ti2m.e63sm-o3r7e.l3i5k.elyThtiosdiefefoefctblraedmdaeinrecdanwcheernthtahne (da0t.a12weexrpeecatneda)l.yzeAdllusoifntghecsoeunwtoyrkdeerasthharadtebs.eenTheimcpelomyaelde aetmtphle oDyeecsatuinr tphleanctfohoorrmtodrieetdhoafnbl2a0ddyeerarcsa,nacnedr amlolrtoaflitthyemstuhday,d w1obrlkaedddeirnchaingcheredxepaotshurweajsorbespofrotreda.t least $ years (SMR = 24.49). In the previous color Texupoosudreaetghrsouwpe.reThreepoSrMteRd ffoorr wloivrekrercsancwehr.o weOrneeewmapsloiynedthien leiotwherexipgoshuroer lgorwouepxpaonsduroenjeobisn wtahes h3i.g0h8 e(x9p5o%suCr1e =gro0u.p3,7-1 i1n1.t1h0e).loFwievxepcoassuerseogfrocuipr,toasnids 2ofitnhteheivnoenr-ewxeproeserde.porTthede oinbstehirsvecdohdoirdt,n2oiextcheeedhitghhe expected mortality experience in anyofthese exposure groups. cMoolsotrtofwatshefsolalmoeweldimiintatthieonuspddaitsec,usthsuesdraebpoovrteinfgortwthieceoraisgimnaanlycodheoartthss;thuodwyeavpeprl,ytthoetcioshorutpdiastef.ailAy yloaurngge.r aTnocdattehde fnourm9b6e%r ooffthdeeatchoshorist,stiblultstmhael6(neostpeocbiaalilnyedforcofuelmdalgeresatilnyalilmpcaatcetgotrhieesr)e.sultDseoafththceeratniaflicyasteess swienrcee twheerenuemxbpeasreodftdoeoatthhesricnhemmoisctaolfs itnhethseuwbo-rckophloarcte.waBsiovleorgyicsamlalm.easIunrdedmietnitosonftolufoluroorcohcehmeimciaclaslsw,ewroerkuesresd m{oisdcelfaisnseifjcoabtsioinntoofexepxpoossuurree.categories based on job descriptions; however, ther is tll a potential for pTohpeulhaitgihonratisiotorfooubbsleesrovmee.d blAaldtdheorucgahncoenrlyde3atdhesaitnhslownegr-teimreepeomrpteldoyieneshitgohtehxopsoeseuxrpeecjtoebsd,itnhteheexgpeencetreadl guimvbenerthein mtahgenigteunderealofptohpeulraitsikoneswtiamsat0e.2(31.2.77It).seeMmasnuynlyiekaerlsy otfhaftoltliosweuffpecwtitwhoouultdanboethdeure dteoatchhafnrcoem, SbtluaddideesrocnanPcFerOSwohualvdehnaovter0epoorctceudrabehfiogrheerthienrceidweuncleodfnoblloandgdeerrbceancaenratpupmroercsia.blIet irsisukn.liCkuerlryentthlayt,(abnaicmcaol SSmmookkiinnggcostualtdusbewasoslenloytredseptoenrsmiibnleedfofrotrhetheixscceoshsoritn;blhaodwdeevrecra,ncleurngmorctaanlcietry,agnidveontthheer 1s3m-ofkoilndgi-nrcerleaastee.d cthaannceirnstwheergeennoertaellepvoaptuelda.tioTnherefore, itis unlikely that tobacco smoking was any higher in he employees. bGliavdednerthceanlicmeirtadteiaotnhsos,fotrhiwshsettuhdeyr, iottihserunccalrecairnwohgeentshemralyuobreocphreesmeinctlsinatrhereDsepcoantsuirbleplfaont. theAtextcheess3oMf CwoetrteagreepGorrtoevde(fSacMilRity1,.w3h1e,re95A%PFC1O=an0d.4o2th~er3.f0l5)o.roFcohuermoifcatlheasree dmeaantuhfsicotcucruerdr,edinbleamddpelroyceanecserwhdoeatdhisd "oTthe waourthkorpsroifmatrhielyDiencattheurchsteumdiycarlepdoirvtisthiaotn tahnebdlathdedrefrocraencweerecaassessuwmoerdtkeodhamovsetllyitilne meaxipnoseunraenc0e jAoPbFsOo.F aatddtihteioninctionNeruaotroorchaenrdmicwaals.tewa1ter itmrpeoarttmaenntt tphlaantfoalnlodwcuopulodfhtahivsecboehoernteixspcoosnetdintuoedmainnyordcehrem(i0cagalisnina better understanding ofthe mortality experienceofworkers expased to florochemicals. In order to "episodeof gain care" aadndailtyisoinsalwaisnsuingdhteritnatkoenthae tehfefeDctescaotfurMpuloarnotchoemsiccraeeln efxopromsourrbeidointywooruktecrosmeshetahlatth,maayn. bixeaassseroiceisatoefdhweiatlhthlocnagr-etesremr,vihciesghperxopvoisduerdeftroomuotrheocshtaermtiocafalspa(rOtlisceunleatradli.sea2s0e01or).conAdniti"oenpiusnotdiel osfoclaurtieo"n ColrirniecsaollutCiaorneofGtrhoautppsro(blCeCmG.) Espoifstwoadrees.ofc~aAlrleiwneprateieindtentainfdiedouitnpaetmipelnotyeveissi'tshetaolthheaclltahimcsarree.coprrdosviudseirnsg,. 5 ENVAMRD(2002)17FINAL opvreorce4d0ur0esd,isaenacsielslaorrycsoenrdviitcieosnsanwderperetsrcarcikpetdi.on drugs used in the diagnosis, treatment, and management of EthpeisDoedceastuofrcaplraentweforreaatnalleyazsed| fyoerar65b2etcwheemeincaJlaneumaprlyoy1, ee1s99a3ndan6d59Deficlemmpbleanrt3e1m,pl1o99y8e.esBwahsoedwoornkewdorskt hainsdtocrhyemrieccoarldsp,laenmtpwloorykeeerss:wGerroeupplaBcheaddinetmopdliofyfeeersntwhcoomopnalryiswoonrkgerodupisn:eitGhrerouthpe Aimconosrisctheedmiocfalalplfainl;m GemrpoluopyeCescownhsoistweodrkoefdeimnplhiogyheeesxpwoshuorewsoirnkethde icnhejmoibcsalwiptlhanthifgohr 1P0OySeFarseoxprosmuorrese;prainodr (G0rtohueponDsethaodf tchheemsitcudayl. plaFnitlemmpplloanyteeesmpwleoryeeaesssuwmeerde oconhsaivdeetrheedhtioghhesatveexlpiotsluereosr.no fluorochemical exposure, while Rbaatsieodsoonf3oMbs'esrveemdpltooyeexepepcotpeudlaetpiiosnodeexspoerfiecnacreewuesrinegcailncdiurleacttesdunfdoraredaiczhatpiloannt.tecEhxnipqeucetse.d nAumrbaetrosofwetrhee Cehxepmeiriceanlceplwaanstscaolbcsuelravteedd ttoo eprxopveicdteedaexrepleartiievencreisdkivriadteod fboyr ctahechfielpmisopldaento'sf ocabrseer(vReRdEPtCo).expec9t5e%d CthoonsfeidwenhciechintheardvalbseewnerreepcoarltceudlatiendafnoirmcaalchorReEpiCd.emioElpoigsiocdelsiotefractaurree tohnatPwFeOreSofagnrdeaPtFeOsAt:intrleisvterwearned balnaddbdielriacrayntcrearct,,eanndodcrreipnreoddiuscotridveerdsiisnovrdoelrvsi.ng the thyroid gland and lipid metabolism, disordersofhe iver "rTehperoodnulcytiivnecrseyassteedmisakndoffoerpithseodoevserfaolrltchasteegcoornyodiftciaonncseorfsaparnidobreniingtenregsrtowwetrhes o(wrhincehopilnacslmudseodfctahnecemraloef talhle4macloempreaprriosdounctgirvoeupssy.stemT)h.eTrhieskrerawtaios waasingcrreeaatseesdt riinskothfeegpriosuopdeosffermpthleoyoveeersalwlictahnctehre chaitgehgeosrtyafnodr Jloonngge-sttimeex.phoisguhr-eesxptoosuuorreoecmhperlmoiyceaelssa(lsRoRwEaPsCre=po1r.t6e,d9f5o%r mCaIle=re1p.r2o--du2c.t1i)v.ecIannccreerasse(dRrRisEkPoCfep=is9.o7d,e9s5i%n Can=d th1e.1s4ub5-8c)a.tegIotrsyhoouflpdrobsetantoetecdantcheart.heFicvoenefpiidseondceesoinftceravrael iwsevreeryobwsiedrevefdorfmoralreeprreopdruocdtuicvteivceanccaenrcserisn ccahnecmeircawlasploabnsterevmepdlionyefeislm(p1l.a8ntexepmepcltoedy)e,esof(3.wehxipcehct4edw)e.re prostate cancers. One episode of prostate "rToheurpe (wRasREaPnCin=cr1e.a8,se9d5r%iskC1of= e1p.i2s-o3d.e0s) faonrdntehoeplloansgm-steorfmtehmepglaosytmroeinntt,esthiingahl etxrapcotsuirnethgerohuipgh(ReRxpEoPsCur=e 2um9b.e9r5s%ofCelpi=so1d.e7s-w5e2r)e.repoMrotsetd oinfftihlem aenpdiscohdeemsicwaelreplaatnbeumtpalbolyeeest.o benign colonic polyps. Similar cInantcheer e(n1t.i5reexcpoehcotretd,).onlByot1 hepoicscoudreroedficnarfeilmwapslarnepeomrtpeldofyolesi.veOrnclaync2ecra(s0e.so6fecxiprechtoesdi)saonfdthIefolrivbelrawdedreer o{bfseecrtivoends(i0n.9cheexmpieccatledp)l,antboetmhplionyetehse,cbhuetmitchaely wpelarnet.masTthleyreducwaos raecgurreiatnegrepiisskodoefsolfocwaerre ubryintahreystaramcet eemppilsooydeeessr.eporIttedis. difficult to draw any conclusions about these observations, given the small number of Cephiesmoidceaslofplcaanrte efmoprldoiyseoredserisnotfhtehehibgihliaerxyptorsaucrtet,halnonthgetierrcmouentmeprlpoayrtmsenint tghreoiupmhpaldant2 (4RRtEiPmeCs=mo2.r6e, 9pl5an0tCw1or=ke1r.s2.- 5T.5h).e sEuibg-hctaeteengoerpiiessodtheasot ficnfalrueenwceerdetohibsseorbvseedrvianticohnemwiecrael pelpainstodeemsploofyecheoslealnitdhi1a4siisn wfiitlhm cepuitseodecshoolecccyusrtrietdisinacnhdemcihcolaelliptlhainatsiesmpwliotyheesc.hronic or unspecified cholecystitis. Mostofthe reported st ENVIMRD(2002)1 FINAL Rdiiasbketreast,iohsyopefrelpiipsiodedmeisao,facnadreotfhoerreennddooccrriinneeodrisonurtdreirtsi,onwahlidcihsoirndcelrus,dewdersueb-noctatleegvoraiteesdoifn tthhyerociodmpdairsciassoen, oCounpfsi.dencCeondiintteirovnals wfhoricthhewehrieghnotexipdoesnutrifei,edlonpgr-itoreimnbuetmwphliocyhmeenxtclugdreoduptheinnculluldehdy:pothdeissiosrdienrstheof95th%e pancreas, cysts, and lower urinary tract infections. "dTehseigrnesaullltsowoefdftohisa sdtiurdeycschoomupladroinsloynobfe wuosrekderfsorwihtyhpostihmeisliasr dgeenmeorgartiaopnh.icsAblutthodiufgfhertehnet eexppiossoudreeso,ftchaerree apraerammaenteyrlciamnintaottiobnes itnotetrhpisredteesdiginn.anEypiostohedresmoafncnearr.e aTrheeredpaotrateadr,endoitfidciusletas(e0 iinncteirdpernecte;betchaeuresfeorale,arthgies RforEepCisomdaeysonfoctanreecehsasadrivleyryinwdiicdaetecohingfhidreinsckeofintienrcvialdse.nceTohfdeiasneaalsyes.is Iwnaasddliitmiiotne,dmtaon6yyoefartsh. rAilssko,rattihoes lmiozraetitohannofonhceaeltihncatrheesedravtiacbeassemabyecraefulseecttlhoecyal cmaendicbael cpartacetgiocreizpeadterunsn.d.erInldairvgiedruaolrs msmaayllbeer cdoiusnetaesde Cilnacsliufdceadtiionnst.heEpdiastaobdaesse,ofscuacrhe amsaythoisnecloudne ltohnegs-taemrem idnidsaibviildiutay.l sevTehrealantiamleyss.is Nmoatyalbleemlpimliotyeedesbywetrhee iSoafgtnwoasreesutsoedt,hewshaimcehcpmiasoyde.misCcelratsasiinfyseerpviisceosd,essuocfh acsarel.ab prTohceedsuorfetswamraey mnoatyhaavlesobeaessnigrnepo2rtdeidffienrtehnet database. 40 Hazards to the Environment aThmemon3iMumC,odmipdaencyyldhiamveethryelpaomrmteodnieucmotaonxdicDitEyAte(sdtietrheasnoulsamionne).fiveThPeFmOaSjorsialytos:ftepsottiansgsihuams, hloiwtheivuem,r cIheensaclatrsrialeldoduitssoocniatthee iPnFsOtaSntpaontcaosussliyumatsalnt.eutOrnallyplHimittoedthdeatPaFaOrSe aivoanilaabnldetfhoer tahpeprtohpreiratsealtcso,ubnutterbieocnausteh.e oirncinotniawlilelxibsetsdfoomrirneaand-tacarnodss.thFaotrrtehaed-psucrrpoosssesofoftthoxiiscirtyeviferwomi tihseaspsoutmasesdituhmatsatlhte ttooxitchietyootfhterhesaPltFsOSi.s ``accocuenpttearbiloensasarae ufrnsltikaeplyprtooxibmeattiooxni,coelxocgeipctalforsitghneifidciadnetccyolmdpiamreetdhywliahmmtohneicuomntsralitb.utTihoinsofistbheecaPuFsOeSthieosne.: aPcuubtleisahneddcthorxoinciictyedfafteat creolnacteinntgratto,iofnosroebxeamp>l1e0,0pmogt/alss(Diiucmri(caksxcahlnodriBdree)da(c0l-fiRsohzeann,d1i9n9v6e;rtBeibersaitnegsershaonwd tChheri3stMenssetnud,ie1s97a2r),e hToowxiecvecronaclelnwterlaltiboensloowfh1e00PmFgO,S psoutgagsesstiiunmgstahlatretphoertpeodtafsosriiumhiaonndwainsvenrottebaramtessjoirn cWoenitgrhitbuotfotrhetoctohuentoevreiroanlsl itsoxciocnistiydeorfetdhteobsuebusntnaneccee.ssFaorry.thDeidpreecsyelntdipmuertphoysleacmomrornecituinonisfaorn etxhecempotlieocnudluacr nthoe opnotleynt0ialitsfhorigehxehribmiotliencgalsaigrniefiigcahntt,tbouxticatlsyotboecaaquuasteichisgphecmioels.ecOularnwtewiolgohtfytamhienesstuadrieeskpnroewsnenttoedhafvoer ivnid ntherewlaatteerd ftoeath(eDsaaplthnoifatmhaigncaat)i,onBothacwuteeresjuuedgsedwittohbteheunFaactcheepatdabmlienfnoorwas(sPeismeipnhgalPeFsOpSrotmoexilcaist)y icsahndiedohfotwheeveursesuogfgeasnt itnhatpptrhoepdriiadteecymledtihmoedtohfyltaesmtmmoeniduimumionpwreapsarantoitona.soTuhreceroesfulstigonfiftihceanttesttoxwiictihtythien the test medium whilst the result otfh test with the wate flea suggested hat it might have been. tWohxeircietvyerofptohsesitbelset smuebasstuarneced.eIxnpossoumree icnosntcaennctersattihoensmehaasvuerebdeecnonucseendtraatsiotnhse wbearses sfiogrniefixcparnetslsyinbgeltohwe nreolmaitniaoln.shIinp bbeatswinegenefPfFecOtScocnocnecnetnrtartaitoinosn aonndmteoaxiscuirtyed concentrations it is assumed tht thre is a direct "aTdhdeitdiaotnatossutmumdayriinefsorimnatTiaobnlersequ7irteod 1u8ndienrctlhuedestcaonlduarmdnsOEheCaDdecdol"uStmundhyeaSdtianngdsa.rd" and "Comments in ss ENVAMRD(2002)17FINAL "scSiteundtiyfisctapnrdoacredd"uriensdiecmatpelotyheedovaenrdallthqeuailniftoyrmoaftitohne creopnotretnedosftutdhy tteasktinrgepoirntt.o aFcicvoeusnttantdhaerdtsechhnaivcealbaenedn used: + = GAcocoedptabthlee study -- the fusltfuildsyails orefquainreomveenrtasllinactceerpmtsoabflmeestahnodda,rdrepfoorrtiunsge ainnd hianztearrpdretaantdionr.isk assessment + aQlutehsotugihonthaebrle-earteheirneadaerqeusaicgineisfiicanstoimneadeelqeumaecnitess. in lemens ofthe study that raise concerns about its + Uvanlaidcictye.ptTahbeleresutlhtesrseahorulsdoignnlifyibceantcoinsnidearedd seuiqpn pueloeamroectnftoiistovhefertshdeastat.udy that invalithdedaattae. = Unknown - there is insuficent informtaoetvailuoatne thestandardofthe study. "cTohnesicdoerleudmnrehleevaadnetd0"tChoemamsesnestssmenhti.ghlRieghatssonosbswehrvyatpirooncsedounretsheorstreusduyltsprwoecreeducroenssiadnedrerdesiunlaspprtohaptriaatrec are also given, "ATnhneetxabl1.esAalssoumcmoantrayinofa tchoelulomwneshteaadcecdept"aSbtluedyefrfeefcetrecnocnecennturmabteiro"nsthfaotr rceafcehrst(ropthhiec lleivselofisstguidvieens iinn Annex4. 41 Effects on Fish, Invertebrates and Aquatic plants (Algae and higher plants) A`ocnuetsepe(csiheosrto-ftesraml)twdaattearwisehr, aavafiislhabalceclfirmoamteadtottoalsoalftw3a1ters,tutdiweosscpoevceireisnogftfhrreeshswpaetceiresanodftfhreesehwsapteecriefsisoh,f Sraelsthwwaatetrerinhviegrhteerbrpaltaen,.tTrheee sstpuedciieesshoafvefrbeesehwnatreerviaenwdedoannedsptheicsiehsasorfessaullttwedatienr2algbaeeinagndcoonnseidsepreecdietso boef oafndanaqaucatciecpapbllanes.standard for assesing the acute(short.term) toxic hazard of PFOS to fish, invertebrates SSunpuiebcc-eiclehlsruolonafircfairlceghsarheow.naitAcerr(epfvirsiohel,owonongfeedt/shopeencgsietersu-tdoesfrmfh)raessdharteaasnuwdletersdaelitanwvasateievlrebinlnevbeefrirtnoegbmrcaaotentsoaitnadlderooefdnetossptbeuecdiioeefss aocnofvfaecrrceiesnphgtwaatbtwleoer MSaunmdbaerrd 2f5orwiatshsestshiengFatthheatdoxmicinhnazoawrd(PoifmePpFhOaSlestopfriosmhelaansd)iwnvaesrtecbornastiesd.ereItd suhnoauclcdepbteabnloetebdectahuasteostfud&y Jraecpkortinnfuomrmbaetrio1n4.iSnttuhdeyre1p4orwt.asIjuidsgperdotbobabelestchcaetpttaibsle.study was the same as that described more fully in mInacardodcihtiionnu,s)flaonwd-tcharrou(gChypbriioncounscceanrtpriaot).ion studies have been conducted in the bluegill sunfish (Lepomis "The acceptable and unacceptable studies are identified in Table 6. so ENVIMRDEOOI FINAL "Taanbdlseu6b.cAhcrcoenpitcaibclheraonndieun(apcrcoelpotnagbelde/lstnugdtieesrmf)or{doextierhmaiznairndgotfhePFacOuSte lo fish invertebrates and aquatic plans algae and higher plants) [|[7 a a arp m om y | [Tees ference numbers| Pp | Lp I Trneafcecreepnicaebniumsbteurdsy 24362) Greshwaten) [pomOis mnacrcocmohyrkairsss||hm3e12,08l2 us|| 3 |(SactuowtaetneGhrio)rticerrmo) noixestoy0sh |ACopginig olnvcrigeatus| i`AcutGerGeshhowrattteermy toxicity to imertcheates (freshwater |Da7phni7amagna____|I 3. 15.1S7.2R5.33 ---- | eewnecheorsttaerm)ntonicy to |Mysdopsis bia ---- 5 5 || Siumbe-rihhrroantierscrhersohnwaovtiecity to |Dapinia magna [ Sub-chronic <hronie trict16 |ysidopsi bia 5.15 ww CII [iincviertcybroatefsreashlwaatterewniceloar |Seerasiran Fae || |stgac ca"iavnpiarcbiuacleoanranuftpeulsnlculaosa B oxic to saitwater wnieeular |Lu"aFnnogxieicrteatromfaroeshxwaytertoWifgrheesrhwater|| SLceaenpnnrasiiicbsoarn 7 =: il plans AL Fish Acute (short-term) toxicity "uHTahnveeirebsseuelntnsdvosfOetndhceofroahrcyutnteecsht(iusnsghom-rytkPeiirsmsme)p(hRsataluidenisbcsopwrarotemreosulu)a.msmTa(rhFieazteehnedaddipn omTinantbnlaoesws7).e.ssTLehderpeionemaislpslectmihaeecsrteooscfthsfirwruaessshm(voBarltutaeglfiiiltslyh during 3 96-hour exposure period 19P.6H-pOhroSoumrteiltLahiseumwasvsaallttuhoeefmo4of1st7.m8seusm.cegpLi.iwbmlaaescrfdoreectshheiwrnamutisen,readfnisdwhiOts.hpemcbyioketishssins,pwaeeccruiteeesoenfslorystmwhaiertghdiineatllholawyneosllteas9sm6isn-uehsocu(eprDtEiLbACl)esaf-nod3r Lipoidtneagsestihumemepstoithtalsrsaeismupmemcotsrailvtie.luyAm. svAaelrsyoefchoiPtnhFdO9sS6t-uwdhayosuornobLtOaLnicnoevrdahlyfuonercohPf.usppprmroyomkxeiilsmsaasstheilnoyawe2eds0t0anomngL/Cwla.tdeeortfear2cm2cionmmegdm/lofdoaargtaheiedn hhisespeecioesyhastrreeceanthlyibeoeinlreynarmeepdorPtrfedooFkiorechsnriillamsuobcfah spcietapto ible orbeter quality, ote most eine alu ed is assessment. 5 ENVIMRD(2002)17FINAL fPrFacOtSiownseorfeannotadqeuteeorumsinmeidxtaunrdeccoounltdainnotibngetehsetismuabstteadncien.thHisowteevter, the sctual exposure concentrations of dPeFtOerSminalesdo fexohitbhietspoatcaustseiutmoxsiaclittyinto tfeissthwiitnhsa0.ltwmaytkeri.ssAacc9l6i-mhaotuerd tLoCsaltvwaaltueer oatfa13s.al7inmigty/lofha3s0 pbaeretns cpoermptahrouesdandw.itThheO.datmayksihsosw lsiavlitnwgateinr afcrcelsihmwaatteerd.O. mHyokwiesvserto,beinofthseimialbasresnucsecepotfibimleiatysurePdFeOxSpowshuerne scaolntcewnattreartisoonlsu,biiltistyho(u2l.d5 b0e 2n0otmedgt,hatdetphesndsitnugdyonmisgahltinhitayveanbdeepnurictoyn).ducAtefdurtinheerxscteusdsoyfutshinegsaubssailatnwcaet'esr fiinsdhicaStheedepasshe1a5dmgm/ilnninowth(eCtyepsrt.inLoodsosn ovfarcioengcaetnutsr)atsihoonwdeudrinnog ttohxeictietsy, uppro1b0abtlhye dwuateertososleudbiimleinttyatliiomnit, indicates that this value mbaeabyove the rue solubily, however, Sub-chronic/chronic (prolonged/long-term) toxicity "HTahveerbeeseunscoafritehde souubt-cwhirtohnitcw/ochsrpoenciiees(opfroflroensghewadtleorngf-isehrm--)Psitmuedpihesalaerse psruommmealraisze(dFaitnheTaadblmein8.noSwt)udaineds Lepomis macrochirus concentrations affecting e(aBrlluyegiiflel-stsaugnfeissho)f. theTeissths ovweirthexpPo.surperopmeerliaosds owferuep tode4s2igdnayesd. Mtoo.rtadleitteyrmdiantea rfooruLp.smoafcrfioschliwreursewererceorodbedaionveedr tfhreom62a-dbaiyocuopntcaeknetpahraisoen osfttuhdey isntuwdyh.ich deaths in the treated and control p"rThoemelloawsesTtodetfhieniptoivteasnsoiumobssaeltrvoefd PeFffOeSc.t Tchoincsenvtarlauteiownas(NaOppElCi)cabolfe 0t3bomtgh/lsuwravisvadletaenrdmignreodwtfhorenPd. npooinstisg.niTfhiicsantNOmoErCtaliistsyupatpoarnteedxbpoysurreesulctosncreontmraatiboinoocfo0nc0e8nt6atmioyn sotvuedryawit62h-Ld.aymaucprtaokceinpuhsastehbautsh1o0w0e%d. pmoorttaaslsiituymatsaaltcoonnceenatrrlaytilofneosft0a.g8e7s omfg/P. apfrteorm3e5ladsaycs.ouAldNnoOtEbCeoaftr1.b0utmeedltroepormtoerdefosrpeecfiffeicctseonfdPpFoiOnSt because insufficient information was contained in the study report, Bioconcentration A0.f0l.o0w8-tahnrodu0g.h8b7iomcyo/ncLenwterraetiuosneds.tudAytotfPheF0.O0S86inmb/lulegillelveslu,ntfhieshfiwsahwsecroeneduxcptoesd.edTefosrt c6o2ncdeanytsr,awthioinlse oaft fthreom0.t8h7e mtegs/tLchlaevmeble,rtshebyfisrhanwderoem esxelpeocsteidonfaotr 3152 tdiamyes pdouienttsodeuxrciensgsitvhee msotrutdayl.ity.TheFyiswhewreereeutchoalnliezcetde,d bfrlaoctttieodndsryw,ewreeigwheeigdhaendd. meTahseurehdea.d,Thfeinyswaenrde dviisssceecrtaedweinrteo ecdoinbsliedearneddnotonebdeiblneonteidsisbuelefatcitsisounesaanndd tthhee craelmcauilnaitnegd ftrisosmuet,heinsculumdionfgthsekiend,ibwlaesancdonnsoindeedriebdletopabret.ediSbtleeadyti-ssstuae.te BWChFolvealfuiesshwceornececnatlrcautliaotnesd wferroem cthoencetnitsrsuaetiocnosncweentrreatcioonnssidaeireadpp0arbeenta sstteeaaddyy--ssttaattee dii3fvidoerdmobryetchoensmeecuatnivewasteetr ocfontciesnstureatcioonnc.entraTtiisosnuse. wfaetreewneortesicganlicfuilcaatnetdlyfdoirfftehreente.dibTleh,e nkoinneetdiicblbeocaonndcewnhtorlaetofnisfhacetxopro(seBdCFtKo),0.u0p8t6akme eraLte uasnidngdeBpuIrOaFtiAoCn computer software. cIlneatrhliys asttauidyn,edPfOoSthebifoicsohnecxepnotrsaetded0i0n.0t8h6 mtigs/sLu.es oAfltbhloueuggihllthseuntfiisshu.e reAspipdaureelntestvoeafedyP-lsFtOasSte wwearse nnoott sitnactriesatiscianlglyuspigtnoiftihceanlatslty ddaifyfoefreenxtpoosnurdea.ysB4C9,FK56 vaanldues6f2o,rtehdeibcloen,cenntornaetdiiobnleofanPdFwOhSolaeppfeiashretdistsouebsewsetrle calculated 0 be 1124, 4013, and 2796, respectively. PFOS depurated slowly. The BIOFAC estimates for ss ENVAMRD(2002)17FINAL rtehsepetcitmievetl0y.reach 0% clearance for edible, nonedible, and whole is tissues were 6, 116, and 112 days, cKaurpriuom).e TLhaebofriasthowreyr(e2c0h01e)ckceodndviuscutaeldlyaafnldowth-otsheroduegmhonbsitorcaotnicnegntarnaytaobnnosrtmudayliotfy PweFrOeSreinmocvaerdp.(CTyhperifniussh wweerree emaerdeidcaftoerdtdoayesliiminnaatfelopwartahsoirtoesugahnsdysttreamnsffoelrleodwitnogaann aexcteelrinmaaltidziisningfeacqtuiaorn.iumA.fterAfrteearritnhge, tsheecfoinsdh wexetreernarlemdiosviendfecatniodn,thteheyrewmeariendaecrcliomfaltihzeed.fishThweerfeishredaermeodnsftorrat1iSngdaanyys aibnnoarmfalloiwtytdhurroiunggh thsiysstpeermioadt tfelmopwertahtruoruegshosfy2s5te+m2foCr.anTohtehefris2h7wedaryes.traTnsefmeprererdattourteestoftatnhkeseasntd rweaatreerdwatasthemesausmueretdempweirtahtuarleconholthe othxeyrgmeonmeptroebreaannddrreeccoorrddeeddtowniccee aadawye.ek.DisTshoelvpedHoofxtyhgeentecsotncweantterratwiaosnsmewaassurmeedaswuirtehdawpitHh maetdeirssoonlveeda wweaeskm.eaTseumrpeerdaatnudreroefcotredtedwtatewrawiawcseemeke.asuDruerdinagndthreeecxopredreidmeonntcaelapdaey. rDhiiesesoxocldrveetd,aoxoyfgceanp,idnirfetsot nwatteesrt ctoannkcse,ntwraetrieonreramnogveeddfornocm7e.a91d0ay. 8.1mThye;teamnpedrtahteurpeHrraannggeedd ffrroomm 72.56.10071.05.25.4C; the dissolved oxygen tFohrotuyghcasrypstweemr.e Texhpeotseesdt wtoattewroofcoenaccehntrlaevteilonwsaosfaPnFaOlSyz,ed2 aanncde2b0efuogr/eLfriersspaencatliyvseilsyofforte8st dfiasyhsainndaatfltohwe eSxapmoesutrie.me aFouarnailsyshiwseorfetetsatkfeinsho.utAnataleyascihsosfamtpelsitnigshimweasanpderdfiovrimdeedd siinxtotitmweosgartocuapcs,h alnevdelthienndubroatthiowneroe.f caonnatleynzte.dgirnodiuvpisduoalflyt.woBfeicsahuwseerteheussetdo.redAnsaalmypslies rofomcoonnteolfifsihswhawsa0s0psemraflorfmoerd tbheefomreeastuhreeemxepnetriomfenlitpaild sitnatrottihngreeangdroaufpts,e tahnedetxhpeenribmaetnhtawlerceomapnlaeltyiozne.d inSdiixvifdiusahlwlyere taken out at each sampling time and divided cAvharloymsaitsogroafphPy-FmOaSss sinpectthreomteetsrty (waHtPeLrC-aMnSd) acnaarlpysisw.asThpeertefsotrwmaetderoufsicnagchhilgohve-lpewrafsoramnaanlcyez.edloiqnucied rbeefaocrheedfiwrshteannatlhyrseiessoufccteessstifvieshanaanldysaetstohfeBsCaFmse mtiamdeeaosntsheamapnlaelsystiaskeonf attheintteesrvaflissoh.f aStielaedasyt-s4a8tehowuarss bweeerne rweiatchhiend+a2ftr 0o28fdc%aaycsh. oTthheer.fisWhhwehnicBhCwFesreweerxepolseessdtfhoart61100d,ayitswwaesreesveaplaurataetdedthiatnao psatretasd;y-tsetgautmeehnatd, ead, viscera except liver, liver and remaining matter, BCFswere determined inal the pars. Thehisfisshtuwdye.rePReOxSpobsieodcofnorce5n8tadtaeydsintothmeatiksesuupesofforcaerxpc.essTeisvte cmoonrctaelnittrya.tionBsioofco2ncaenndta2t0ioung/Lfacwteorres uwseerde.. calculated tobe 720 for 20 ug/L. and 200- 1500for 2 ug/L. 50 or=E . men ] Sei || gEg,T --w --n og Notnoed =| nrCo 47 im Totsubstance was PFOS imsl. E = l Sena eE EeeT oe mie Sem re Err oo | `Quesintie| Te vasFt OSstma aiss. Rm os se 16 2632426) ~ eTe E reg| EE[e w E| &T =Ee|ReTE |E|E Er [[eEEEE eo ESareRe E,Ee Lapomismacrochis| OEC20D[SoborLCu=78 | Ace|TetsubwsasiPOaSnDEcAmei = sm | SE i E peEeer r E sEE Orcsmys|Nox ed(i)|96Com11 ==. a ms thal of aud Tost bracewasPROSpom ` [EE]==|| ===5i eoermTEoRg[ E E E fro Er a mm |om Opin vargas| OPPS | SeLCi15n ma =EX Em EEEi o EE Gato = SES EESeEefErEe.iEn Oncorbumehusmpliss SehrCy = 137) Tot acewas PFOS poasensh. oeta weby oye TestsubstancewasPROSpotas salt. sw swamomsipoprmymtienas o TeEr ETeE]e [T Ea wE ee] Tom) em| EEfD |Simere E E EE s E TE E= | day NOC E=e t [5 Er E [Toms Fro sates. Ww EEEEeEE e -- i -- ENVIMRD(2002)17FINAL 412 Invertebrates "`TTahbelerses9u:lasnodfa10c.ute(shortterm) and sub-ehronic/chronic prolonged/Tong-term) studies are summarised in Acute (short-term) toxicity tofreshwaterspecies "UTnwioo scpoemcpielsamoafufsre(sfhrweastherwaintveerrtmeubsrasteel.haIvmemobbeielnsuasteidofno/rmotretsatlinigy -duDraipnhgn4i8a-hmoaugrnsaex(phoesu"rweatwearsflaesas'e)sasnedd cinomtphleamtaesttusswith D. magna. Morality during 96-hours exposure was assessed in the test with U tAhelowdeaspthnaicdc.e"ptaAbledS4-8h-ohuorur EECLs vvaalluueeofof27ampgp/rloxwiamsatdeeltyerm4i0nedmfogr PFwaOsS paoltsaossdieumtesralmtiningtesftosr witthhe dimidxetcuyreimceotnthayilnianmgmotnheiusmubssattanocfe.PRHOoSweivnaedra,phthneidactteusatlonewxaptoesruarecccoomnmceondtaratteidonfsraoctfioPnsFoOSafnwearqeueonuost ddeitdeercmyilndeidmeitnhytlhiasmmtoenstiuamndm,ayas hpaovientecdontoruitbuitnedtthoe tihnetrtoodxuicctiitoyn otfo tthheistesstectmieond,iuimt.is Tphosessieblevaltuheast `compare with a 96-hour LC. value determinedformartalty ofthe musofs59emgl. Acute (short-term) toxicity to saltwater species TCrharseseosstpreeciaevsirogfinsiaclatw(aEtaesrteirnnveorytsetberra)teanhdaAvretebmeiean supse(Bdrifonre tsehsrtiimnpg).- MMoyrstiadloiptsyidsurbianhgia96(-Mhyousrisd esxhproismupr)e, w96a-shoaussresesxepdosiunrtehepetreisosdwiinththM.tebstahwiiathanCd. Avritregimniiacas.p.ThReedtuecsttisounbsitnasnhceellwdaespoPsiFtOiSonpwoetsaassssieusmsesadltovineralal AMy9s6i-dhoshurrimLpC..Tvhailsuceoomfpa3.r6esmgw/)itaahnd9a6n-haosusrocEiCat.eqdvNalOuEeoCfo>f3.1.01 mmgg//llfwoereeffdeecttesromninsehdellindtehpeostietstiownitihn tthhee oyster nda48-hour LCi of 8.9 mg for mortalityofthe Brine shrimp. Sub-chronic/chronic (prolonged/long-term) toxicty o freshwater species T"wwaotertesftlsea'h,avDeapbheneinacmaraigenda.ouTthoenePndROpoSinptostaasssseisusmedsalitn wtihtehteasntes wseperceiessurovfivfarle,sghrwaotwetrhiannvderrtebprraotdeuctitohne measured over exposure periods ofup to 28 days. rNeOspEecCtSiveolfy. 12Inatnhde 271-mdga/yl theastvethbeeeNnOEdCetserfmoirnesdurvfiovralD.anmdaggrnoawtrhepwreorduectailosno i1n2 2m1g,andind2i8c-adtaiyngttehsatts reproduction was no more snsitive han these twoother end points. ta*Fhloesuse lDi aabpcahssneidtaoomwnaatngeonrmaaictahcleormeexmaporodesautirevedeco4fna8cc-ethniotournrast.iaocTnuhst,ee brtueoxtmiacsiiintnyicnesgttu4hd8ei-ethsoocuorkf sEscoCleuestpitoabnelwe2a7o,fp5b5re.etpe6a1rreqdnuadaltit2y1c,0oanmlctpeh.noruTtghihoeonnleatthoaetrf eixtcheecdoemdpawraatbelresomluebialy,soeuxfptorosiuecreys,coancnedsntoratthieonmsorseskeenlsitovbaeluleowiveretdhainthisnaomsinsael,smTehinst.avesJus Tre reports o ENVAMRD(002)17FINAL `Sub-chronic/chronic (prolonged/long:term) toxicity tosaltwater species sAhrtiemstp,haMsysbiedeonpsciasrrbiadhioau.t oTnhePFenOdSppooitnatsssiausmsessasletdwiinththoenetessptecwieersoe fsusravlitvwaal,tegrrionwvtehrteabnrdater;eptrheodMuycsiiodn measuredoveran exposure period of35 days. The mel 3r5e-sdpeacytiNveOlyE. C determined fo survival, growth and reproduction in this test were 0.55, 0.25 and 0.25 6s [or] Fe o[2ore] | a ,T| gT [ERw = EEEa | fE Sa o Ee mEimE Ie. E FEr ET | E Ee SfoiEa re E mEEm = er SEommer ir So| Twa r|Er||gg[E =E SE-- rT eEm S agl.] mrE sEr om | er 1 EEEiSzem ae meer em EEmSEE E [eme| FE eeESE oEaAE vEEa EEEEEa EeCT||n m[E m Ee EtT Ea e e eRe re aiEg| sim dayECan12 | Accepible |TostsubstancewasPROSpoasisamt. i 02s. - ENVIMRD(2002)17FINAL 41.3 Aquatic plants "hTihgehreersulptlasntosf).allNotthee: shTohrte- aspnedcileosngSeerl-etnearsmtrstuumdiceasparriecosrummumtaurmisheads ibneTeanblreesna1m1edandPsc1u2d(oakligraceh)naenrdill1a3 subcapiata. Fo transparency, the name used in th tst report has been retained in the discussion below. Freshwater alga-e Short-term ATnhraebeaesnpaecfileoss-oafqufareesshnwdatNearviucnuilcealpleulllacruallogsaae. hTahveeebnedepnoiunsteadssfeosrsetdestiinntghe- tSeeslsenwaasstrgurmowctahpmriecaosrunruetdumi,n atsesremsssoefdcteolxlicdietnybsiytyr,efgerroewntcher1a0teefafnecdtosronthgraorwetahurnadteearrtehceognrsoiwdtehrecdurhveereofovrert9h6e-hpouurrpso.seOsnolfdyestteurdimeisnithnagt ethxeprteosxsiceidtyoreflatPiFveOS10tcoelallgnaeu.mbTehresraesnudltcseollfsdtyudwyeinguhmt.beTrhe13searrestuhletsrfcoorueldexbcelucdoendsibdeecraeudsewetrheeytahree EoClsy wviatluhefsistho, tbheisriescanloctucloatnesdidienrerdeshpeicgthofprgiorroiwtty.h rat, but since algae were relatively insensitive compared "HTohweelvoewerstth9e6re-haoruersEomCe vunacleurftoairnetfifeesctosveorntghreovwatlhidaittyeooff t7h1ismgre/slulwtagsivdeentetrhmianeedxfpoors5ureccaopnrciecnotrrnautitoun.s fwoerrmeulaottionm)e.asAur9e6d-haonudr EdieCthyvlaelnuee oglfyc1o2l6 bmugt/yll weatsherobtwaaisnepdrefsoernttheinsathmeetsepsetcimeasteursiianlga(asteptartprootfoctohle tthhaetsewwasojtuedsgtesdwetroem3ee5atnadl4t4hemc/rliterreisapefcotrivaeclcye.ptTahbielitteystosfwittheh dthatstwToheot9he6r-hsopuecrieNsOyEiCelsdeadss9o6c-ihaotuerd EwCi.tyh vmaellues of 176 mel (4. los-aquae) and 305 mgd (N. pellculosa) and respective NOECs of 94 and 206 Saltwater algae - Short-term "ATh9e6t-ehstouwragsruonwatbhleinthoibdiettieornmtienset ahadsefbieneintivcear9r6i-edhoouurt EonCPFvaOlSuepobteacsasuisuemnsoaletffweictths wSkeerleedteotneermmaicnoesdtaarutnh.e highest dissolved PFOS concentration that could be attained under the test conditions (3.2 mg). Freshwater alga-e Longer-term O11n0.e13s)p.ecTihees otefstfraessshewsasteedreufnfiecctesllounlagrraolwgtahe rhaast bexepernestessetdedre-laStliveena0stcreulmdecnaspirtiycoovrenrut14ud(asyts.udyThreefreerseunlctes rdeonsmittyhe(secsetcaroemmneontdissciunssreesdpefcurtothfershheorertfoer ntwotesrtes)a.sonSse.coFnidrsltyl,y,tthhrereasrueltscoanrceeerxnpsretshsatedtehelt1i4v-edatyo ctelslt pteirmieo(dhaendteastbsmeendcieoafwetersetnmoetdainuamlyrseendewfoarlPmFaOyShacovnecernesturlatteidoni.n sThdeeclliatnteerinisesxupgogseusrteecdobncyecntormaptairoinssoonveorf the 14-day EC value of5 me with the 96-h EC.q value of82 mg! determined in the same test. Freshwater higher plants tAesgtryoiwetlhdeidnahi7bi-tdiaoyn IeCsta hoafs1b0e8enmcgarrfioerdionuhtiboitnioPnFoOffSropontdaspsrioudmucstailonwiatnhdLae7m-ndaaygiNbObaEC(Doucfk1w5e.e1d)m.g/lThe based on the inhibition offrond production and evidenceofsub-lthal effects. Er] me Tam] we Te [TT-- Tat. Accarta psecdtfo PROSdeterminedin sts with alse Fo [a mr |TOoRD |e howBee er | Pr fsooeE eta a0l Om |m amp .th BRari keres iT bEamr E T n | Er |frTb FOS e jR rPocek nA rmreTaeR treema, fa ott Se EE Pe TT aeo oo [ ElE mEle eE En]| =[Te -- E ammmmme e [[x+] [EE im Eh|~ [mma |v] ewan Tai. Subckrnchmteregtongrm ds HOS din fs ih es Species. "Protocol Resales (mg) on Comments [t=] = SRSA |G | Rn Emm. RON Tae 15. Ast hrm por xt to PFO deredfenih tr lens [|w en[m ret| eer i [a] ENVIMRD(2002)1 FINAL 42 Effects on Other Aquatic Organisms 42.1 Amphibians DpoattaasssiuummmasarlitsweidthiXneTnaobplues 1l4aevwiesr(eAforbitcaainnecdlaiwnedanfroegm).brEyxopotseurraetoogefntehseisemabsrsyaoyscafrorri9e6d hoouutrsonresPuFliOeSd icnoncaenntLraCt.ionfotrhatmoirnthailbiittyedgorfo1w3t.h8wmasg7.a9n7dmga/nl. EaCnds thfeortemraaltfogoernmiactiionndsexwoafs1c2a.l1culmagt.ed, Tahstehemirnatiimouomf tbheea9d6e-vheoluorpLmeCnta0l htahzea9r6d-hinoutrhiEs sCp,ec0sbe 1.1. The later value indicates that PROS has a low potential to n ENVIRO TAL Table 4, Toit 02a orPHOS determineid 0embry eaogemess avy with the phil,Newopis ci| [Cm|r J ro Roig Commer Seg eoNpere diet Sehmoie p Sim n = 121| APH T[TosiTs okev eorssFrOPROSSppr n:CL a ENVIMRD(2002)1 FINAL 422 Sediment Dwelling Invertebrates Ncooncdeanttaraatreioanva(iPlNabElCe)fo foerffseecdtismoennts-eddwiemlelnntgdwienlvelritnegbriantveesrtceoburaltde.beItdsetpeorsmsiinbelde tbhyatappprleydiincgteedqnuioleifbfreicutm puanrltiikteiloynitnogbemofdeeaslisblteoatthtehedaptraesefonrt wimaet.er column organisms. However, as discussed in Annex 3, this is 423 Bacteria rDeaftearesnucmemanruimsbeedrsin1T8abalned 12S2)w.erTeheobtMaiicnredotforxom p2rorceepodrutrsedeassscersisbeisnglMigihctroouttopxut sitnukdiibesit(osntufdyrormeptohret elxupmoisneusrceepnetrimoadri(n3e0bmaicntuetreisuminPthhoetocbasaecotferhiuensephtowsophstourdeiuesn., Bfooltlhowsitnugdieexspaorseourfeatno auntaocxciecpaintabolveesrtaansdhaorrdt cfoorncaesnstersastiinognsthaendhaiznarodneofcaPsFeObSec(aiutsheiuomf tshael)prteosebanccteeroifadibetchayulseeneofgluynccoelrtianintthyeotvesetrstahmeplter.ueTehxeproesuarree athlissopmaortriecugleanrergarloucpoonfceorrngsanoivsemrst.heNroelteovxaincceeofffctthsewteertesoybssteermvaedndintecstitspherctieesst faotrndoemtienramlincionngceenftfercattsiofnosr (>250 my) that ml depending owneprueristiygsnailfiinciatnyt)l.y in excessofthe solubility of PFOS salt in saline medium (2.5 to 20 7s ENVIMRDGOITFIAL Tabs. Tovkiy doafor POSdecrminedin Micros ss Species | Protocol | Results ma) Comments | hiPcohopnaisr)nm|||MMBiacosoibcni:s|| 1mSmaitnee EE+CC.252500 | Ursa| RITeosnntibsnoeckxapaowosraesncPoeEpnrOceSpmati rteidnosnictnsionth was Ww pKiaelyebcoacpxnoeonnnpdeirsceuybnloi ign)ncliwsneehswmainrhspeo1s5i1t0d30k. ShrineiEC.L 130 sResiinsnproacb eofoeioeaio ND Toossmhiae xrpeowsasu ci oFnOceSntporafoc tiisonsio.fde iyegs ol 0 = dTSolsitecomsoinmc3.31r0 e30tm1epix enic noofgnslosb in R{apdecyulisoigcroombbeiteietnheeF hiy d iosayf15 ENVAMRD(2002)17FINAL 42.4 Activated Sludge Microorganisms Dinahtiabitsiuomnmsatruidsieesd (isntuTdayblreepo1r6t rweefreerenocbetaniunmedberfsro6m, 319reapnodrts21)d.esOcnrilbyinsgtuadcytirveafteerdenscleudnguembreesrpi9ratwiaosn caonndsi21derheadd tsobigeniofficaanntaicncaedpetqaubalceisetsanidnatredtfoprroascseedsusriensg rtehleatheadza0rdoteft PcFonOdSititoonasc,tdiuvartaetdiosnluodfget.heSteuxdipeoss1.9 andor absence ofa reference substance. "(TnhoemihnaolucroncIeCnt.ravtailonu)e. for PFOS (potassium salt) determined in the acceptable study was 905 mg/l " ENVIRO ANAL Table 16. Tosciy dotafor FOS determined in Activated Sade Respiration abt ess [atom SonSdaayrd Commer RtSwaNyo. SAhceteed |OFCD 207|ShouIC 5905 (em) NT {ocmedi hxo tponweoaetscPct oOonScpmoetaah ns toifrs0aie9tth9i05omnongs OECD|sim iC 238(ns pTostrbeanvceiwaos FOSpots si f= Te Ro tsempe peiuorrsfescon aarpnodliaps idon0sn0n,d.llwel on mm Ounesnbeariainnrvihsaoiucry.ding he fs30 N"TpoosomtiurtpaxnipcedowssncaPonOecSerDtbEyoAcss.ui rasans. l NNootmrbermbearemmtoh, ENVAMRD(2002)17FINAL 43 EffectsonTerrestrialOrganisms 43.1 Solk-dwelling Invertebrates eNfofecdtatcaonacreenatvraaitliaobnle(PfoNrEeCf)fefcotrs soinls-odilwedlwienlginingveirntveebrrteabtreastecso.ulIdt ibsehdoewteevremrinpeodssbiyblaeptphlaytingpreeqduiicltiebdriiuom upanrltiikteiloyntiobngemofdeealsisblteoathteh daptraesfeonrtwiamte.e cIotliusmnnotoerdgatnhaitsmasn.eaHrotwhewvoerrm,aacutdeitsocxuiscsietdy ainndAnupnteaxke3,Sthuidsy iiss in the planningstages,and a ina report shouldbe available in 2002. 432 Terrestrial Plants cNooncdeanttaratarieonav(aPilNaEbCle) ffoorretfefrercetsstroianl tpelraensttsiaclouplldantbs.e Idtetiesrhmoiwneedvebrypoaspspilbyliengthetquailpirberdiiucmtepdarntoitieofnfiencgt t`hmeodperless0entthiemed.ataIfoiranqoutaedtitchatplaatnetrse.sHtroiwalepvleanrat,stodxiicsictuyssaenddiunptAankneesxtu3d,ytshis his uboneliaukvealilylatbodlebeinfe2a0s0i2b.le at 433 Birds ADnataas spulmamtayrriiysnecdhaisn,Taabnlde t1h7eweNroertrhoemr 2Borebpwohrittsedesqcuariilb,inCgodliitnaursyvaicrugtienisatnuudsies(swtiutdhythreepMoartllarredfedruecnkc,e nobusmebrevratsio1n1sawnedre12)m.adBierdasfeirn b3ootrh t1e7stdsaywse.reBoetxhpossueddietsoaPrFeOcSonspiodtearsesdiutmobsealotfian thaecicedpiettasblfeorstadndaayrsdafnodr assessing the acute toxic hazardof PROStobirds via dietary exposure. vTahleuelowwaesstaapcpurtoexidmiaettaerlyyLhCalaf tvhaaltueobotfai2n2e0dmign/tkhge oefsftoowidthwatsheddeutcekr.miTnheed lionwtehsetteNstOwEiCtohfthe37qumaigl./kTghiosf fwoaosdafpoprerfofxeimcattsoelny bhaoldfythwaetiogbhttaiwnaesd,wiintchontthreoastth,erosbpteaciineesd inthteheqtueasitl.ith the duck. Once again this value Ii noted that avian reproductive data willbesubmited for eviews in 2002, " ENVIMRDOITFAL Fable 17. cuediary ity datfo PFOSdtm fst ith birds Tost species Protect Resultos(ama+gof |suSntiudayra Comments RSewtaNyo. PDnleiandtdeys|| O3E50C2D2E0005N8OFFRFAS|N[NLOCOaC-aC25m=e14367 Ne Sdeasyssxpoeaxrpceoofololor waedr3ra0i17oodyonsd.. Gms ||OEC=DRS OMFS [L=2C0 Good |TSosotmaabann wasPOSomain. SNaraamm | SIIOBRaFIRA|NNOEoCaroa7er3 Bones NSSeiaverecasanpoponflcioedcbeyt3r0s17iodoyosn. ui ENVIMRD(2002)17FINAL 43.4 Bees HDaotnaeysubmemear(idspeids minelTliafbelrea)18onwePrFeOoSbtpaoitnaesdsiinumacsuatlte. oTrahleasntdudcioenstafcutlltyoxmiecitty ttheestsrecqauriireedd souttandwairtdh tfhoer aceepabiltyofthe dat 0".T2h1e agchuteee.oraTlhteestcoynitealcdtedtesat7y2i-ehloudreadLD9i6g-hooruriLngDeisgtoifon of4.7P8 FgOSbof0a.n40dai9/6b-heoeuranNdOaE7L2-ohfou1r.9N3 OpgEiLbeo.f aTdhmeinirsestpeercetdivveiaLtheDse rvoaultueess. indicate moderate and high orders of toxicity of PROS to. bees when 5 ENVIMRDQOITENAL Tab 15 Actra and coat it dt foPROSdetermined cst with Honey bs Totpedes| Proton SeSatnaayd Commer RStNyo. Coimpel"a|| OEECoOImD. on || PTaoANhGoEn"03oo0h r sNownenaexpor nes ol--l"i0%e pnienyeos || orOEEsCODs2I1sa.o||OofkhhoouwwrNLO0EL7-159| 3 Gond |NTSoeintbiaetadancpeswiaesPoisRnsOoeSonpionrsesehcdraelscs. sioof cnst a Conse) ENVAMRD(2002)1 7FINAL 50 References S3uMmmRaerpyorotfs:Hu"mThaen SSecriae,nHceealotfh,OarngdanTiocx.icFolluoogryocDhaentmai"s,tdrayt"edanFdebr*uarPeyr5f,lu1o9r9o9o,ct(aSnEeHQS-u0l2fo9n9a-t3e7:3)C.urrent 319M99.ComSpEaHnQy-.0310999-.374SEHQ-0281~A-dd0it3io7na4l Information. Submittedto U.S. EoPnMaArch 15, e3ffMectCso.mpSaunbym.it2e0d00tao. USSulEfPonAatMeadrcPehrf1,lu2o0r0o0c.hemicals in the Environment: Sources, Dispersion, Fate and v3aMriCoousmpsaalntyf.or2m0s0.03b.MVCoolmunptaanryy ussuebmainsdsieoxnpotsoutrheeiUnSfoErmPaAt,io2n7prAopfriilf2o0r0p0e.rfluorooctanesulfonic acid and `3CMompCaonmyp.anFyY.L02700000c-.13D7e8t,er8m(ien)atSiuopnpolefmSeenrtaulmSuHablmFi-sLsiivoens, oSfEHSQev-e0r3a7l3F/l0u3o7r4o.chemicas, June , 2000, 3M 3M Company. 2000d. Phase-out Pan for POSF-Based Products, Submi1t0eUdS EPA July 7, 2000 a3nMd CRoegmuplaantyo.ry2A0f0f0aei.rs)V.erIbnaflormCaolmmOeEnCtDs MbeyeWtiilnlgitaomDWiespcpusrserPF(OSDItialorfAEsensveicsrsomtnemnetontRaerplo,rt.HeaOlctdo,bSearfe2t6y, 2000, S3eMawCaotmepraannyd.3.250%01S.odEinuvmirCohnlmoreindtealSoLlaubtoiroant-orSyhaPkroejeFcltasNkuMmebtehord.EOO3-M17C1o6m.paWnayt,erSStolPuabuill,iMtyN.in Natural S3hMakeCoFmlpaasnkyM.eth2o0d0.1b.3MEnCvoirmopnamneyn,taSlt PLaaublo,rMatNo.ry Project Number EOO-1716. Solubility in Octanol 3M Company, 2001. #HQ-998-374. Submitted to US EPA on March 25, 2001 S3uMlfoCnoimcpaAncyi,d 2P0o0t2a.ss1i0um4-SWaeletk(DPiFeOtSa;ryTC6h2ro9n5i)c iTnoxRiactist.y aFinndalCaRrecpionrotg,en3icMityT6S2tu9d5y (wiCtohvaPnecreflusotruodoyctnao.ne 6320-153), Volumes 1X ,4068 pages, January 2, 2002. 3M, St. Paul, Minnesota. 3M Environmental Laboratory. 1993. 3M Lab Request Number L3306, 3M Company, St. Paul, MN. a3nMd ECnovnicreonntrmaetnitoanl LoafboProattaosrsy.ium19P9e9raf.luAonraoloycttiacnaelsuLlafboonraatteor(yRCeApSorNtumobnert:he 2D7e9t5e-r3m9i-n3a)tioinn tofhteheSePrruemsenacned. LiverofSprague-Dawley Rats Exposed to PFOS via Gavage. As amended April 19, 2000. P3rMeseEnocveiraonndmeCnotnacelntLraabtoiroantoofryP.erf1l9o9r9o6o.ctanAensaluyltfiocnaalteLa(bPoFrOaSt)or(yCAReSpoNrutmboenr:th2e75D9e-t3e9r-m3i)naitnitohne oSfertuhme Noof.Sp2r7a7g9uc,-RDeaqwuleesytoRraPtrsojEexcptosNeod. t3oMPoTtoaxss6i2u9m5.1P3e.rfluSotruodoyctiannietisautlifoonndaattee:viJauGnav1a0g,e,199L9a.borCaotmoprlyetRieopnorstt s1i08g,ning (2/11/00). Sample analysis completion June 28, 1999. Study Identification Number: FACT ToxS3eMdimEennvti,roPnOmeTnWtalEffLlaubeonrtaatonrdy.Lan2d0f0i1lal.LeaEcnhvaitreonSmaemnptlaels. M3onMitLoarboirnagtor--ieMs,ultSti.-CPiatuyl, SMtNud,y:JunWeat2e5r., Sludge, 5 ENVIMRDQ002)I7FINAL SLaMborEantvoirrieosn,meStn.taPlauL,abMoNra,toMrya.rc2h0011.b. Summary of Naive RavRat Chow/Fish Meal Study Analyses. 3M 3InMtemEantviiornoaln,meLntdt.alAvLaibaonraatnodryA.qu2a0t0ilcc.FeEecdoMtaotxriScteusd.ie3sM~LPabFoOrSa:torBiaecs,kgStr.ouPnaudl,ScMrNee,nJiunneSe2l1e.cted Wildife 3DeMteErnmviinraotniomnenotfalthLeaboCroantcoernyt.rat2i0o0n1do.f APnoatlaystsiicuaml LPaebrofrlactrooroyctRaepnoerstu,foFnAatCeT(TPOFXO-S0)99in, FReabbrbiutarLyi9v,er20a0n1d Serum Samples. L3aMboErnavtiorroynRmeepnotratl, LDaebtoerramtionrayti2o0n01oef.thOeraPlre(sGeanvcaegaen)dPChoanrcmeanctorkaitnieotnicofStPuedryflouforPooFcOtSaneinsuRlaftosr,atAena(lPytFiOcSa)l SitnudSye:ru#m4,18L-i0v1e3r,, AUnrailnyet,icaanldReFepcoerst FSaAmCpTleTs.OX-31M10,Me3diMcaLlabDoerpataorrtymeRnetquSetsutdyNo.T-U6228954.91,2,MaAyrg4u,s20I0n1-Lifc 3`AMnalEyntivciarlonLmaebnotraatlorLyabRoerpaotrotr,y D2e0t0e1r.minOartailon(oGfavtahgee)CoPnhcaernmtraactoikoinneotficPeRrefcluoovreoroyctSatnuedsuylofofnPatFeO(SPFinOSR)asi,n tEhneviSreornumme,ntaLilveLra,boUrraitnoer,y aRnedporFtecNeos. oTfOXC-1r1:1,CDBLRaboVrAaFt/oPryluRseqRuaetsst ENox.poUs2e9d94t,o 3PMRORSef.viNao.GaTv-a6g2e0,5.314M, May 4.2001. RAelpeoxratn.der,DivBiHs.ionAopfriElnv26i,ro2n00m1e.ntaMlortaanlditOycsctuupdaytoifownaolrkHeeraslthe,mpSlcohyoeodl aotfthPeub3lMicDHeecaalttuhr, fUacniilviteyr.sitFyinoalf Minnesota, BReelqiuseks.tJ.#A1G99758.07I;RPDrCoje1c3t79-019722:77F0C0-0945./Mo3nMkeCyo.mpTaencyh.nical Report Summary fromJ. BelisletoJ. Long rBeipersiondguecrt,ionKEa.nd amentdabCohlriisstmenosfenD,aphGnMi.a ma19g7a2.. JEoffuercatls ooffthvearFiiosuhserimeestaRlesseaornchthBeoasrudrvoifvaCl,anagdrao,wt2h9, (12) 1691-1700, BNoi.e4s1e0m2e5i71eJ,rAW,.AaRnFd HIanrstiist,utDeLIn.c. 1974. Eye and Skin Iitation Report on Sample T1117. Report. Project BNoocvienmgbe2r00107., 20L0e0,ter from Boeing Company to USEPA re: Docket Control Number OPPTS-S0639. iBnaSruimsJi.tOolmsoen3MG.eWm.p,lMoayneedse.l JFiHn,alSRcehpourtm.pe3rMJt.C,C.om1p99a9n.y,De3tMermMiendaitciaolnoDfeSpaerrtumemntF,luEopriodcehmeimoilcoagly,Le2v2e0l.s W05. FYLOS00-01378, BMuuorrsoc,heImMi,calOsl.seInn,teGr,imSRiempposrotn,#1.C,3MManMdeedli,caJ.l De2p0a00r.tmenD.eteFrYmIi-na0t7i0o0n-1o3f75serum half-lives of several Bofursresv.eraJlM,fuLournodcbheemrigc,alJsK., OIlnsteenr,imGRWep,orSitm#p2s.on3,MC,MeMdaincadlelD,eJ.par2t0m02e.nt.Determinationofserumhal lives ChemSaurces USA: 2000 Editon. ChemSources Intemational, Inc. Clemson, SC. Christian, M.S.. Hoberman, A.M, and York, R.G. 1999a. Oral (Stomach Tube) Developmental Toxicity u ENVAMRD(002)1 FINAL EStYuLdOySo0f0-0P1R3O7S8,in Rabbits. Argus Research Laboratories, Inc. Protocol Number: 418-012, January 199. CDherviesltoapnm,enMt.aSl.,anHdPoebreirnmaatna,/PAosMtn,atalanRdeprYoodrukc,tiRoGn.Toxic1i9t9y9.StudyCoomfbiPnFeOdS OirnalRats(.GaAvraggues) RFeesreilairtcyh, 0L2ab0o0r:a0t0o3ri7e4s), In. Protocol Number: 418-008, Sponsor Study Number: 6295.9, June 10, 1999. (SEHQLCharbiosrtaitaonr,ieMs.,S.Inc.HPorboetromcaonl, NAuMm,bearn4d1Y-o0r1k4,,RSpGo.ns1o9r9:9.3MTeCsotripnograFtaceilTiotxyi:coAlrogguys, RSetsuedayrcNhumber T6295.13. July 23,1999. Oral (Gavage) Cross-Fostering StudyofPFOS in Rats CRiepfoornte., CMo.vA.anc1e99S9.tudUynsNcoh.e2d0u7l8e4d04D4N7A. CSoyvnatnhceseisLaibnorRaattoriLeisvelrc,PrViimeanrnyaC,eVllirgCiunlitaur2e2s18w2i.th PFOS. Final SDuerafna,ctaWn.tP,FCJ-e9s5suApc,utDe.C.O,ralThToomxipcsiotny (G.L,DR)omSitgu,dyG.i,n Raantsd. PSotwueldly,NoD.. 1139778-.083,FlIunotreamdatFiolnuaolroRcehseemairccahl and Development Corporation. Diericks, PJ. and Bredacl-RozenE,. 1996. Bull Environ. Contam, Tosical. 57, 107-110. DirecotfWoorrlyd Chemical Producers: 2000Editon. Chemical Information Services. Dallas, TX Gmaireisney mJa.P.mamnadlsK.. KParnenpaarne,d20f0o1r4.3M,AcSct.umuPlaualtiMoNno.fPJeurnfelu2o0r.ooIcntaUnSe.sulEfPonAatAedamnidnirserlaatteidvefluRoercoocrhdemiAcRa2l2s6i.n 10307 bGiirdes.sJyP.reP.paarneddKf.or K3aMn,naSnt., P2a0u0l1bM.N.PJeurnfel2u0r.ooIcn tUa.nS.esEuPlAfoAndaamntidneisretlraatteidveflRoercoocrhdemAiRc2al2s6-i1n f0i3s0hAcatingwater fGiishestiJyss.uePs..aPnrdepKa.reKdanfnoran3,M,20S0t1.c.PauAlccMuNm.ulJautnieon20.ofIpnerU.fSu.orEoPocAtaAndmeisnuifsotnraatteivaenRderceolradteAdRf2l2uo6r-o1c0he3m0iAcals in mGiineksaJyn.dP.ivaenrd oKn.esK.annParnep,a2r0e0d14f.or A3cMc,umSut.laPtaiuolnoMNf.peJrufnleuo20r.ooIcntUa.nS.esEuPlfAonAaadnmtdienirsetlartaetdivfeuoRreoccohredmiAcRa2l2s6i-n 1030 CGriaesssyostJ.reaP.vieagnidnicKa., fKraonmntahne,G20u0o1f.MlexPeifrcfoluarnodocCthaenseaspuelfaokneatBeay.anPdrepraerlaetdefdor3flMu,oroSc.hePmaiuclalMsN.inJuonyest2e0r., In US. EPA Administrative Record AR226-1030A 3GMi,esSty. PaJ.Pu. laMndN,J. PLr.ojNeecwts1t7e8d4,01.200J1ue..SeIlneUc.tSe.d EfPluAorAodcmhienmiisctarlastiivnethReecDoercdatAurR,22A6l-a1b0a3m0aAarea. Prepared for TGooxlidceinttyhaSlt,uEdyL.,SJteusdsyupN,o.D.C1.3,7-G0e9i2l,, IRn.tGe.maatnidonMaelhrRiensge,ar1c.h5.an1d97D54e.veNlionpcmteyn-tdaCyorSpuobraactuitoen,RhMeatstuaswaMno,nkMeI.y FYLOS00-137S, SGtuodyl.dSet,udyEIN,o.Jes1s3u7p-,08D5.C,.,IGnetiemla,tiR.Go,nJaelfRfeesresaornch, aN.nDd.DaenvdelAorcpemoe,ntR.JC.or1p9o7r8abt,ioNni,neMtayt-tdaawyaSnu,baMcI.uteFYR1a-t 0500-1375. 5s ENVAM/RD(002)1 FINAL GToolxdiecnitthyalS,tudEy.L,StJeusdsyupN,o.D.1C3,7.G0e8i7l,, IRn.tGe.rnaatnidonMaelkRreisnega,rcJhS.and197D9e.veNlionpemtey-ndtaCyorSpuobraactuitoen,RhMeastutsawMaon.nkMe1y FYLOS00-1375, GLoabrotreart,oriEe.sG,. In1c.980E.xpOerrailmeTnetraNtuomlboegry:St0u6dSy0ToRfOF0C0-S9,5DeinceRmabtesr.,Sa19f5e0t.y FEYv1a-lu0aSt0i0o-n01L3a7b5o.ratory and Riker cHhaanrsacetnerizKa.tiJo.n oHf.raOceJloehvneslosno,f JP. FSO. SEalnddriPdFgOeJ,A. iLn. thBeutTeennhnoefsfs,eeanRdiveLr.. AE.nviDriocnk., S2i0.02T,ecQhunaonl.tit2a0t0i2v,e 36. 1681-1685. SuHlaftofniaetledT.(.PF20O0S1).. Sc3rMeeEninnvgirSontmeuntdoailnteLahsbeorAaqtoureyo.usLaPhbotroelqyuteisct DneugmrbaedratWi2on7o7f5P,oAtparsisli2u3m,2P0e0r1f.luoSrto.cPtaauln,e AN. aHcaidug(PhFoOmAB),,.apnedflOuyosrtoeoins,taSn.e1s9u92l.phoTnhiecmaeccidha(nPiFsOmSuA)nandd celofrtihberlihcyypaoclid.ipneBmigioccheifmf.ectBoiofpphryfs.uorAcotoac.ta1n1o2i5c, 6572. 4e5d0a,anTd,pFeurkouxdias,omKe.,prMoolriife,raIt,ioEnnoimn ortaot,liMv.e,r Komai, T. and Suga, T. 1987. Induction of cytochrome by perfluorinated octanesulfonc acid. In: Peroxisomes Pin Biology and Medicine. H.D. Fahim and H. Spies, Eds. Springer Verlag, New York, 304-308 JDooshen.sonR.ik1eDr.,LaGboirbastoonr,ieSs.,J.Ica.n.dSOubebrs,iRdF.oifa1r937yM9,9.St.AbPsaourl,ptMiYon.ofPrFoCj-ec9t5N-o1.45C9i0n31R0at2s00aftear Single Oral JoofhTnostoanl, JC.aDr,boGni-b1s4on,in.RJa.sanadfeOrbear,SRiEng.le, 1I9n7t9r5a.veEnxotuesntDoasned RoofutFeCo-9f5E.x1c4rCe.tionRiaknedr TLiasbsoureatDoirsitersi,buItnico.n Subsidiaryof3M, St. Paul, MN. J1o4hnsoinn, J. RDa.s,GiafbersJoA.dnmain,ndiOsbteari,oRnE. 1984. Cholestyramine-Enhanced Fecal EliminationofCarbon of Ammonium [14C|Perfluorooctanote or Potassium [14C]Perfluoroocianesulfonate. Fundamental and Applied Toxicolog4y: 972-976. KurumeLaboratory 2001. Chemicals Evaluation and Research Institut,Japan, Testnumber: $1520 sKuulrfounmiec Laacbido,ratteosrty s2u0b02s.tanFcienalnurempboretr, bKi-o1d5eg2r0ad(atteistonntuemsboefrsa2l1t5(20N)a,. KK,uLriu)moef pLearbfolruaotrooarlyk,ylCh(eCm=i4c-a1l2s) Evaluation and Research Institue, Japan. DLaeuv.elC.o,pmReongtearls,tIoxMi.c,ityHaonfspoenr,flRu.oG.r,aocBtarabneese,ulBf.oDn.a,tNea(rPoFikOyS,) Mi.ntG.heSrcathamnidd,moLEu. saen.d RTiecrhaatrodlso,g1yH.63:2020910 (P49), LPliattoenTeBsit.oneFtiinasl, RIenpocr.t.197L9B.IMPurtoajegcetniNcoi.ty20E8v3a8l.uationofT-2014 CoC in the Ames Salmonella/Microsome Mandel. J. 2000. Memo regarding OUS pooled samples. 3M Internal Correspondence, August 2000 Mecehi, M.S. 1999. Salmo--nEscehelrilchaia coli-Mammalian-Microsome Reverse Mutation Assay with 56 ENVAMRD(2002)17FINAL PROS. Final Report. CovanceStudyNo.:20784-0-409, Covance Laboratorics, Vienna, Virgina 22152. Moody. CA. and J.A. Field. fire-fighting activity. Environ. 1S9c9i.9. TeDchneol.t33e: 2r8o0m0f-p2ei5r0f6nluoaroctarbioyolatnes in groundwater impactedby `MCuHrV,StH.udy19N9o6.. M1u7t4a0g3e-n0i4c5i5t.y TCeosmtionng THa-z6l2e9t5oninIca.n(ICnHVVi)voViMeonunas,eVMiirgcirnoinau2c2l1e8u2s. Assay. Final Report. Muri, Report. H. 1999. Covance CShtruodmyosNoo:mal27A8b4e-r0r4a4t9i.onsFiinnalHuRempaornt. WhCoolveancBleooLdaboLryamtporhioecsytIensc. wiVtihennPaF,OSV.irgFiinniaal 21%. NMaabsbteefreslTdh,esDi.s,19U8n8i.veArsnitiynovefstMiignanteisoontoaf.thTeheesfsfercetsseoafrcfhlupoerrofcohremmeidcaaltsaonndsluipvpeor rftaetdybayci3d MbiCnodimnpganpyro.tin. Nacaibdibaenfealldo,guDe., fBruomtefnathtoy faJ,c,idBcaasrsr,ieNr. praontdeiSnseabcyawtAy,.eth1-91948.,64D3i,spalmacmeomnenitumofapefrlfulouroersocoecnttalcyatl,abpeolteadssfaitutmy pToexrifclouloorgcioscttan1e995s)ulfonate and. other known peroxisome proliferstors, (SOT Abstract. Accepied `PD Chemical Buyers Directory 2000, Schnell Publishing New York, NY. OAlusgeunstG1W1,,19L9o9g.anF,luP-oWr.o,chSeimmipcsaoln,ExCp.oAs.u,rHeaAnssseens,sBmuernrtsoJfMD.e,caBtuurrlCehwemM.iMc.a,l aSncdhuFmipelrtPlIaCn,t EMmapnldoeyleeJsH.. Final Report, 3M Medical Department. FY1LOS00-01375. pOlesrefnluGoWr,oocButralneewsuMlM(fF,oFnOaBStu)reriasndJMpe,rflMuaonrdoeolctaJnHo.ste (20P0FlOa.A) inArelcartoisosn-steoctciloinnailcalacnhaelymsiisstryo,fthsyerrouimd Ahonrtmwoenrep, ahnedmatDoelcoagtyuranMduourroincahleymsiicsarlesumlesdicfarlom smuarvleeilalnadncefemparloegreamm.ploFyienealparretpiocrti.pants3oMf thMeed2i0c0al0 Department. POlesrenf.luGoWro,oeBtuarnlecws,alM(fPMoFn,OaStB)eurasn,d PJeMrf,luoMraonodcetlan,csJtHe.(PF20O0A1)b.LeveAlsLionngRietluadtiinoanloAnLailpyisdiasndofHeSpeartuinc FClliunoircoaclheCmhiecmailstMreydicTaelstSuRrevseuilltlsancferoPmroMgraalme. E3mMplFoiyneael RPepaorritc.ipants of the 1994/95, 1997, and 2000 aOlnsaelny,sisGoWf,epBiusroldeews,ofMcMa,reHoock3fiMngD,ecBaBt,urSkcrhaettm,icJaCl,aBnudrrfisi,lmIpMl,antMaenmdpello,yeJeHs.,21090913c-.199A8.n epFiindaelmiRoelpoogritc. May18,2001 ODlessecnr.iptGiWv,e sLuomgamna,ryPWo,f sSeirmpusmonf,luCorAo,chBeumirciasl, JleMv,elsBuralmeown,gMeMm,plLouynedebepragr,ticJiKp,anMtasnodfelt,heJH.year20201040.0 Decatur fucrochemical medical surveillance program. Final Report. March 19, 2001. JOHl.sen.200G1We., DSecshcirmiipctkilveer,suMm,mTaireyreonfss,eJrMu,mLfolgoarno,chPeWr,icBaulrilsev,elJsMa,mBounrgleewm,plMoMy,ee Lpuarntdibceirpga,ntsJKo,ftMhaendyeela,r 2000 Antwerp fluorochemical medica surveillance program. Final Report. March 19, 2001 lOelvseelns,aGmWo,ngM2a3ds6ebnu,ilDdCi,ngBeumrprilso,yeJeMs,. MFainndaellR,eJpoHr.t. 2M0a0r1.chDe19s,cr2i0p0t1ive summaryofserum fluorochemical wo ENVAMRD(2002)17FINAL OHlusmena.nGTWi,s.HanFsienna,l CRlepeomretn., ELpAi,deBmuirorlios,gyJ,M,22M0a-n3dWe-l0,5,JH.Med2i0c0a1l. DIedpeanrttimfeicnatt,io3n MofCFolmupoaroncyh,emSit.caPlasuli,n MN 55144. Oflusoerno,chGeWmi,caBlusrriins,hIuMm,anLusenrda.ber1g. , AJmKe,rHiacnasnenR,edKJC,roMsasndaedull,tJHbl,ooZdobdeoln,orL.R. Fi2n0a0l2ar.eportI,den3tiMficMaetidoincaolf Deparment. AOlusoerno.chGeWm,icBaulrriins,huImM,anLusenrda.ber1g1,. JEKl,derHlaynspeanr,ticKiJp,anMtsanodfelt,heJHA,duZlotbeClh,anLgRes. in20T0h2obu.ghtKeSnttuidfyc,atSieosnteo,f Washington. Final Report. 3M Medical Department OMlusoerno,chGeWmi,caBlusrtisn, hJuMm,anLunsedrbae.rg,ILJ.K, PHeadnisaterni,c. KpJa,riMcainpdaentls, JinH,3ZoGbrelo,upLRA. Si2r0c0p2tco.socKcieniclficnaictailontrioalf investigation. Final Report. 3MMedicalDepartment. tRhuesRacth.G,PMr.ojeRcitnNeoh.ar7t8,-7W1E8.5,aBnidoB/odzyanakm,iCc.sA.Inc.1979. AnAcute Inhalation Toxicity StofuT-23d06CyoC in PSeearcfaltu.oroActMa.nesuAlnfaolnyiteicAaclidLPaobtosrsaitourmySalRtep(oTr-t629f5r)oimn Cythneomo2l6-gWuseMeoknkCeaypssoulne thTeoxDiectietryminSattuidoyn oftwihteh PDreepsaerntcmeenatndStCuodnyc:enTt-r6at2i9o5n7o;fCPoervfalnucoeroIonc-tLainfeesuSltfuodnya:t#e63(2P9F-O22S3).inAnLailvyetriacnald SSteurduy:m SFaAmpClTesT.O3X-M03Me0d;ic3aMl Laboratory Request No, U2279. SCoiCmamnadnTV..-22F.4819C7o8.C.InFinVailtrRoepMoirctr.obSiRoIlPorgoijceaclt:MuLaSgCe-n44i4c2i-y01A6s.saSyRsIofIn3teMmatcioomnpalanMyenCloompPoarukn,dCsalTi-fo2r2n4i7a 94025, sSuolhfloenniicusa,ciAdKis,4Eproitkesnstoni,nAduMce.r oHfopgersoxsiosoCmm.,a,l Kfiamtltyanadc,idMB.-oaxniddaDtieonPiaernrde,otJhWe.r a1ct9i9v3i.tiPeserkflnuoowrnoocttoabnee. affected by perovisome prolferators in mouse liver Pharmacol. Toxicol. 72, 90-93 T(PhRoOmSf:orTd-,62PJ0.5)1i9n98C.yn4o-mWoelegkusCaMposnukleeysTo.xiUcniatuydiStteuddyDrawfitt.hStPuerdfyluNoor.oc6tan3e 2Sul9ffo-onri2e3MA2c,idSt.PPoatuals,sMiuNm,Sbalyt Covance Laboratories Inc. Madison, WI. FY1L0S00-1375, T(hPoOmfSo;rdT,62PJ9.5)20i0n0.C2y6n-oWmeolsgkCuaspsMuorliekTeoyxs.iciUtnyauSdtiutdeydwiDthrPaerfFlinuaolrooRcetpaonretSuPlrfeopraircedAcfiodr P3oMta,ssSitumPaSuall,t MApirninles1o2t,a200b0y, CFoYvLa0nSc0e0-La1b3o7r8atories, Inc. Madison Wisconsin $3704-2595, Study Number 6329-223 UAuSgEuPstA1919999.9. New pesticide fact sheet lithium perfluorooctane sulfonate (LPOS). EPA-730-F99-009, Wsaatlllaivceer,mKi.tBo.chaonnddriSat.rkDoevp,aAr.tm1e9n9t8.ofThBeioecfhfeecmtiosftrpyeraunodrMionlaetceudlaarryBliaoklyolgsyu,foUnniavmeirdseistyonofbiMocNnerSgcehtoiocls ooff Medicine, Duluth, MN $5812, USA. Suppobyratreandt from 3M Company. WPreotjzeeclt,NLuTm.ber1:9831.34-R1a6t0,TDeeracteomlboegyr 1S9t,ud1y9,83T.-3(3S5E1H,Q.F0i3n9al9-R3e7p4o)rt.. Hazslon Laboratories America, Inc. 5 ENV/IMRD(002)1 FINAL Amst. Ecological Studies "1T2heJfuloy 2l00l0,lo2s5swoJifusntneu2dg0y01r,epaonrtdsFweebrreuacroyn2ta0i0n2ed on a 3M Fuorochemical EPA Submissions CDs dated 117 29J6ul-yHo2u0r00Sac Acute TosicityTest with the Fathead Minnow (Pimephalespromelas) 32.. 4986--HHoouurr STtoaxtiiccitAycuTteestTowxiitchihtyeTFersetswhiwtahtetrhACllgaad(oSceereannas(tDraupnhnciaaprmiacgonran)unu) 45. 9966--HHoouurr SSthaetlilcDAecpuotseitTiooxnicTietsyt TwietshwtihtehEtahseteFrrnesOhywsatteerr(CMruasssseolst(rUenaiovicrgoimnpilcaam)atus) 67.. 9A6ct-iHvoautredStSatuicdA,cuRteespTiorxaitciiotnyITnehsitbiwtiiotn TtheestSaltwater Mysd (Msidopsis bahia) 98.. SEeaarnl-ySLtiaftei-cStLaigfee-TCoyxcilceitTyoxTiecsittywiTtehstthweitFhatthheeaCdlMadioncneorwan((PDiampephhnailaemsapgrnoam)elas) 1110.. DFileotwa-rtyhrLoCug.hSLtiufdey-CwyictlhetThoexMiaciltayrTdes with the Saltwater Mysid (Mysidopsis bahia) 1123.. DMiuelttair-yPhLaCse.ESxtpuodsyurweit/hRethceovNeorrythAelrgaBloAbswshaiyteTest 13. TSuhreviEvfaflecotfsForfyCoofntFiantuhoeuasd AMqiunenoouws(EPxipmoespuhrieal0sp1r4oCm-e7l8a.s02/oSnumHmatacrhyaobiflhtiysotfopEatghsolongidcaGlrowth and 15. eExffaemcitnoaftPiootnassosfiuFmatPheeardfMlinunoorwo(oPicmtepahanleeossnpuSrulormfveiolvanasl)a,eteeex.p(oDaspohendidr78e.02prfoordu3c0tiDoany)s 1176.. 4Pi5m-eHpRhaAlceustperoTmoxeilcaisty9t6o-hDoauprhnTioax,icDiatpyhTneisat mDaagtnaaS.umFmCa-r9y4.-XSa(LmiplsaeltFoCf-9P4F-OXS)(Li saltof PFOS) 1195.. MEviaclruoabtiicosnMoifcFroCt-o9x4-ToXvibcyitOyETCesDt. AScatimvpalteed :SFhCu-d9g4e-RXe(sLpiirsaatltioonfIPnFhiObSi)tion Test #209 ReviewofOECD. 20. 9260-9HnoudrBAOcuDtIeCTOoxDicTietsytTReesstuolntsBflourcFgCi-l9S4u-nfXi.sthes(tFCda-t9a9,shDeeEtAs (sLailtosafltoPfFOPSE)O) 2212.] MAiccurtoetTooxxidcaittayftoor FAcMt.i3va8t2e0d(S2lu8d%gePF(FOCS-)99, DEA sat of PFOS) 2234.. TAocxuitceitTyox1i0cAiltygateo (DSaeplhenniaasimraumgncaapfroircForMn-u3t8a2n)0f(o2r8F%C-P3F8O2S0)(28% PFOS) 2256.. FDiantaal CformopmreFhaetnhseiadveMRienponrotw: FSCt-u9d5y on FC-93 (25% NH, salt of PFOS in IPA and water), 3M 27. EDnavairofnrmoemntaFlathLaeba,d AMugi.nn2o,w197S4t.udy on FC-93 (25% NH, salt of PFOS in IPA and water), 3M 25. EAncvuitreotnomneinciitaylofLaPb,30O2c5.de19v,el1o9p7m4ental material to Fathead minnow (Pimephales promelas) 29. Acute toxicityofP3023 developmental material to Daphnia magna. 3205.AJcuuntee2t0o0x1icity ofPFOS to Rainbow trout in saltwater 3312.. AAccuuttee toosxiicciittyyooffPPFFOOSS ttoo ARraiienmbioawpr.out n freshwater 3334.. APecruftleuowroxoioccittayonefsuPlFfOoSnatt,oPDoatpahsnsiiaummsaagtn(aPFOS): An acute oral toxicity sudy with the Honey bee 5356.. PPeOrSfl:uorAoo9c6t-ahnoeusrultfooxniactiet,yPtoetsawsistihumthsealfr(ePsFhwOaSt)e:rAalngaa(cAunacboanetnacatfltoosxi-caigtuyaset)udy with he Honey bee 3357.. PPRFOOSS:: AA 976--dhaoyutroxtiocxiitcyittyestteswtiwtihtDhufcrkewsheweadte(rLdeimantaomgi(bVbaaviGc3u)lapelculosa) ENVAMRD(200F2IN)A1L 4300.. PPFFOOSS:: AA f9r6o-ghoeumrbrtoyxoitceitryattoesgtenweistihsatshesamyar--iXnendoipautosm((FSEkTelAeXt)onema costatum) 31. PBerlfluloro(oLcetpaonemsiuslfmoancartoec,hiProitsa)ssium salt (PROS): A flow-through bioconcentation test with the February 2002 42, PRearilnuboorowocTtraonuets(ulOfnocnoarthey,ncPhoutsasmsykiiusms)sailntfr(ePsFhOwSa)t:er 96-Hour Static Acute Toxicity Test with the 43. PSehreleuposrhoeoacdtaMniesnunlofowna(tCey,prPiontoadsosniuvmarsiaelgtar(uPsF)OSi)n:sa9lt6w-aHtoeurr Semi-Static Acute Toxicity Test with the % 2 4 6 [17 [20 Z 332 3 :34 ;:39 a T48HRAcuteToxicity to Daphnia.Daphniamagna.FC-9%-X (Lisa of PFOS) "| T96-Hour Acute Toxicity TestonBluegillSunfish(FC-99.DEAsaltofPFOS) | BEPO A96-S hourt: oxicitytestwiththefreshwatearlga (Anabaena flos-aguac) a NTE . ENVAMRD(002)1 FINAL MRionbnusotw S(tPuidmyephRaelpeosrptroRmeeflearse)nce No. 1 ~ 96-Hour Static Acute Toxicity Test with the Fathead TTEESSTTSSbUSBSTTAANNCE OMdcetnatnicys:ulfoPnoitcasacsiidu,m 1p.e1r.f2l.u2o3r,o3oc.t4a:n,e5s.u5l.f6o.na6t,e1;,75.m8ay8-heapltsaodecbaefluroerof-e,rrpeodtatsosiausm sPalF,OCSASor# 2F7C-9935-.393)(I. NsRoeMrmeRadrkausnnd:deeTrlheaemmebtnieteatnlstuacnboasnltdyaisnteciseontissecaphrnwiihoqriutteosptoewstdienrg.. PSuraimtpyldeetwearsmiankeednfofrboem930.M49lo%t mbyumLbCe/rM2S1,7,'HSLaHmVpRl,e w"a1s METHOD -- TMyepteh:odS:uriOEaCcuDte203 and OPPTS 850.1075 YGeLaPr:coYmepsleted: Study completed 1999. Reportcompleted 2000 ASSunppeacplilyeitsei:rc:aPliImmnoe-nphioatulosreeisncpgurl:otumPreelFsa,OsSW. mielaesuInratetem0ad,ti4o5n,al9,6-Lhiodu,rsEaston, MD, USA EbSxtipantooimssituairlcaelppremoebratibhoiodldi:st:9y6w-LihtCohusrnsvoanlluienseacarlicnutleastepdo,lawtihoennuspionssgitbhlee,cboymppurtoebirtsaonfatlwyasries,ofmCoEvi.ngStaevpehraange method or "LTeesntgtfihshanadge:weAipgphrtox3i5ma(t3e0l-y381)2m6 mda,ys0o3l6d(021-049) PLoraedtirnega:tm0e.n2t4: gNoinseh "Test ConDdiiltuitoinons water: 0.45 um feredwell water DilutionHawradtneerssc:hemist1r3y1(d(ur1ing2th6emdg--/wL1eaeks3Cpae6rCi)Ood,immediately preceding the test): pAHl:kalinity: 18737 (176mg./L1as7C8aC)O, TCooCnd.uctivity: 3<11Lo3m10e-L315) pnhosiem wmSititoxhcekddilfauontrdi~on2te2wsathtoseuorrls(u0tpiporrnepipar0reoueptsharer.aftioAoufnrt:earddPmiritiximoiannragyl, pestrsoitcmckaornpycreesnpttaorrcaektdisooinln.utdiilountiwoanswpartoeprorattio2n7almlyg/dLiluatnedd S`tCaobinlcietnytroafttihoenstdesotsicnhgemriactael:sOolnucteions: Extremely stable dEexpptohsauprperovxesismealts:ely251L7.6 pcomly.ethylene aquaria containing approximately 15Loftest solution; water NNuummbbeerroofffirsehplipcaetresr:eptlwioc.ate en `NWuamtebrecrohefmciosntcreyntdruartiinognst:hefsivteudpyl:us a negative control Dissolve7d.o8x8y.g8enmgra/nLg(eco(n0t-ro9l6exhopuorssu)r:e) 0 ENVGIOMRFINDAL PHrang$7e37(2-059%0m(ogumr.es)l(8emxpgosee)xpose) Tost tem25p4e0r74a5t-u53re3Gr81anmgeeeol0moplo5npheoo)nus) Meoftcalchu2l13atoingm2e2d3anCmeGahsmuarled coxnpocnentm)teaton:itmmceianc ResuLTs EMNleoeammsienunartledacioonenc.ecnet(nr0ar5tai9ionocson:nsiNkSeng0cie0see,3cm3.otn56,g.93e56.n,5197r,.29i49,map16).,27 mglL SShahooowruLLLCCoaannn7023833mmaEilty((Cm1Taonot rcacusae) All cement alSeisoBonsConyah9e5.a60se1dmCool.ens tnTSoattcaektidsoitefimcmnaoolnresbaayttyeiu.dtiaToovhneero7pfatm2aoreihraotsonaCnl,asb:leCvPoraanlnfuigdeieswnaaocsufessfpteormdncsanb0toierodlbe2ed4a.estneTdch8heehc4o7oumraos2nrdctou4iLl%odpannooounttrdebetLScieoasuacenuses0sto ort DhowLC diatthetmipicernsfiormnmehgaetnhScoedaonlucogtdyi:oAnCnhosofymhaseo5ogfatpeashtybsoalwuintihonisThwaeesressppeserofclorarotmmoenedst.nachcWdsoisecdioonn.IdnrGmsIiLeoOnMpsS.Ltd. Wwpiinntkg CPiroeelnrslcpedeocnntitseedaecaoStorcotrepmtyeerrnouftocmaroromaopotoncnaehnonaetdrs.lscoTumhartecesLdOvaaQsvn(aalieulsetsy.ofCfoqoNmonu8r5ti.oe3nnytootd1nuw7ane%sg0Toa5fma8pndloememoinSasnl rnhMyesseswtr9eyd7s"omTnoeoes BSoaumrpslersanagekde1atm45$7hoes 8ne5edoof omr8i6..3 0 101% f nominal Messe ses Forms oot [------ CoNncoamniinrtailonTesmty. | M5e5.as4unrde9d D6upsli,catResVpaleucesealt,0,|| MCeaonnMeaasnur,ed|| No Percent of [L egine Gonil |------mgA/L ll=1mg0o/gL 6 = -- [ 3s6s 3 3.16,3.53,3.08,322,346 313] 33 | 92 519592.03471.05880.95336, 01780-0T5o2s]] 0 056 --= 205.285.270.078. 268.266] Tos 1cBoin6lcgeminciar5.lioonmbasupepreveaaxtrpieodonsnseor,amfa1tle0r1S09o6m0-m.h5oamurel:lo,Fifsh4he4rin1it7henmegyFaaihovtweeesrcovsntwolesranvdehtdhee S3o3 mol.aexpyose ENVIMRDOOITFINAL Cumulativepercent moratity: [ViCaonncWeneiiornd Test | haus Thos| Shon i REE}T I0 To | f I o os17 I 0ToTo Tw { = CoToTw [iw MLoorwteasltitcyoonfcecnotnetartoilosn: cNaounseing 100% mortality: 28 mg coxcLusions wwTihsteh3p33o9ms5gs%uLcmonpfeirdefnocreoionctieravnalesoffon8t.e01916-mheoulr.LTChefo9r6-fhatohueradnamimnornaolwitwyaasnddetneormoinseds onbece9n.5tumtgolnL Subir: 3M Company, Environmental Laboratory P.O. Box 33331 St. Pau,MN 35133,USA DA ATA QUALITY Reliability: Kiimisch ranking - REFERENCES eeeeee Thi study was conducted at Wilde eration Li. Easton, MD at he requoefsthte 3M Company. onir ee Last changed: $300 0 ENVAMRDQOO2)I FINAL ReolbeunsateoSpturdiycRoempourttoRne)ference No. 2 = 96lor Tost Test with the Freshwater Alga TEST SUBSTANCE Went: PrOcotosnesssulaimnnipcearcuiodr,ooc1a2nc2su3f3oa4t4e5s5m6a0y7al3s8b.e8rheefpearaPeE,OdS xpFo9r51il, CAS RaesmaSrtokrse:d underThaembtisenscuobnsdiatniconisspwhirt0tpeooiwnc.rPSaarmtpyldeewtaemsianekde0fboem330M49o5tCpture.217.vSiampnle "*F-NMRand elementalanalyses techniques. S METHOD ACO rn . A TMoetohoSdi:eOEsCeDe201, OPPTS 850.340, ASTM 1218.90 SYGpeLeacriceVosemsSpleeltenda:sSrtucyacpormpilcetoerdn199, Report completed 2000 nSitnaucrvcuera:slOrmimgodinniamltloyraifnoWg:miPTFdhOSCtumlmetuasrseoeCndollat10o.d7n,29Eofa6tAolnghaMDt.thSeAUniveosfTietxays a Aus, mained SEExltpeomrseunerteebpae1sri9i:o5dRse9pporoteodr ways: mbof cells, rc under hs growth curv and growth te ETAnnSaCydoiercgasaln4imso1mns6it%boorirnagt:oTreystcculotnucree:raAiloganescmeuasrusrehdaadb0e,e7n2 sacntdi9ve6lyhogurros.win i fester sa ulure FSIokdaCondafigodtrieaonceshe:aesmttitworweecvksesproom0a1p 6 inionwT .llSwtorck,mies Soins were perc wing Testtemperature range: 23.6. 25.5C Growth medium: ASTM Suandard Gide 215.505, 1990 CCoagmepcloeauinhood| eEn t]mifi ] Sac5h0;a] Inc, 0a15t5e6 |-- p-- i] 3[ ----FecNiarMeoTiOroI)G B T7a26x Ig a or ~ NCpuaCENoiaDoisT,tAeoMGTh oe aoiz [ m p f r ] mee n ] { Kiro, Loi NOT us ENVAMRD(002) FINAL Cy TT TmDiheleduitpuiHomnowfwatashtepermreepdsaoirueurdmcbew:yaasWdiadldidjnlugisftteehdeI0natpep7mr5ao2tpir0oi.na1atleuvsLoidnl.gum1ew0sel%olfHswtCaoTtceakrndnpuuttrhrieifemineteddsoibluyumtirwoenavsestrsseepruolrsiimzfoeisedidsbw.yeltTlhawetaittoeersnt St.o2c2k3ma)ndprifoersttosuosleu.tion preparation: A. concentration of 183 mg/L. The primary psrtiomcakrysosltuotcikonsowlaustiosntiwrraesd pwrietpharaedmaignneatlgiacl smierdipluatme. atfoar mcaopelpdorrioluxesimsm.a(t0elpyre2pa4rehoutrhse.fAifvteeraddmiitxiionnagl ttheestpcroinmcaernytrasttioocnks.solAultlionfinwalastepstropSoorltuitoinoanlslyspdpielautreedd wciltehaalognadl E1x0p0omsulroefvteesstelsosl:utSitoenr.ile 250 mL polycarbonate Erlenmeyer flasks lugged with foam stoppers containing NAguimtabteiroon:fSrheaplkiecnatceosn:ttihnrueoeusly at 100 rpm e`InsNituteimdablearlogfaclocnecllenltoraadtinigo:ns1.0: iXx 1p0lucsaelnlesgmatLive control plus an abiotic control at the highest concentration Water cPheHmirsatnrgye:(0-96 hours) 7 7.5-8.4 1 ((1c7o9n- tmrogl/eL7 xepxopsou5 rseu)re) Test tem2p3e6ra-t2u5r8erCange (0-96 hours) Light le3ve8l7s0(0--496610holuurxs)from continuous cool-white fluorescent lighting `Methodof calculating mean measured concentrations: arithmetic mean RESULTS eee NMoemaisnuarledcocnocnecnetnrtartaitoinosn:sN:e<gaLtOiQv,ec5o.n5t,r1o1l,,251.,74,41,1,862,3,14769,, 9116,91m53gm.g/L plus 13 mg/L abiotic control 2hElhoeomuuerrntEEvBaClCue(:c(ea9lr5ade%unncsdoientrfy=ic)duer1n6vc3ee=)(in7t41e2-r21v9a(l11)i9s-mg1gi7/v6Le)nmign/bLr.ackets) 4284--hhoouurr EECC. ((cgerllowdtehnsriattye)) == $1136(7(230-9200)4)mmLg/L. 4488eehhoouurr EErBCCay,((garroeawtuhndaetre)cu=rv1e4)2=(1407(-6718-5)14m6)g/mLg/L 7722--hhoouurrCEtbyCocealrleadeunnsdietryc=)ur3v7e()=<046-6(4<)0m-g3/6L) mg/L 7722--hhoouurrtiECayp (c(elglrodwentshitayt)e)==7053(4(423- 7-68)4)mgm/LL. 7722--hhoouurrEExbCCign ((agrreoawutnhdreratceu=)r1ve2)0=7(4103(-551-32)2)mmgL/.L 7T22h-ohuotuFrCEybpCo(c(earledaeunnsdietrcy=u)rv1e5)3 (=131065-(116455)-mg17/6L) mL. 997662-h-hoHouoururErECECiBgaCn,(ca(egrlreodawentushnidtaeytr)e)c=u=r4v91e)(74=93 4-m90L4) 0m(-yC1/3.L0)nmogt/cLa.lculable) 96-hour Co(growth rate=) 59(54- 63) mg/L 9% ENVAMRD(2002)1 FINAL 999666---hhHooouururErECEraBCgCa(c((eagrlreodaweutnnhsdrieatrtye=c))u=r7v1e1)(2=666-71(71(1356).7m-13g78/4)L)mmgg//LL. 9966--hhoouurr EEDCC(c(earledaeunsnidtye)c=ur13v7e(=)101545- (15132)-5m15L5) mg/L 9762--hhoouurr NErOCEopC((grgorwotwhthraatte,=)ce>l1l7d9enmsigty., a(rCe.a. unnodtecralthceulcablue) r=4vmeg/)L 96-hour NOEC (growth rte, cel density, area under the cur=4v4meg/)L Al element valuesbasedon mean measured concentrations Sittnhahetinibsiattniiacolanylvzaemldeutebshyowdleisrn:eearcCaeillncltuelrdapetonelsdattuiiscoisn,ngusa"irTnchgaeTuSOnAdXeSSrSTytAhseTtegmVreforowsrtihoWnicn3ud.r5ovwteso"vc,asltRuieemlsae,taesgetrho6ew.1tE2h,CT,rahteeEsseCvaa,nlduaenspdewrEeceCrnset ``vgSarhlouaweptsihrarona-tdWeis9ka5st'%72fceosatnnfdaind9ed6nchBeaorultrilsmeiwttsteraettesa7tl,2soraeensvdpaelc9tu6iavtehelodyu.rfs.oThnCoeerlmtarldeeiantusymietaninteds,ghraoormueopagssenuwenedireteryotfthhveeanrgciroaomnwptchaersceuudsriv1ne0g attnhhdee control using Dunnett test. Resultsofthe statisticalanalyseswereusedtodetermine the NOEC values dAHiengathl.eymtipineciranflgorMtmehatenhccooednoclelnoitqgruyai:dtiAocnnhaorlfotymshaeetsotogefsrtatspesuhtbysstoalwnuictteihoinnsmtawhseesrteessptpeserocfltourtroimmoeentdsr,iatcth WdiselatdemicefteiaonnIdntm(eomHsaPttLipoOnrMaoSlm)iLn.ied.ntWuphseieannkg rpineedrsicpveoinndtsuearlefcioorsvoempreeyrrifoclfucomoramotporocinixeannfetosrstu.ilffTiochnaaettioeLnsOwQaansa(lliyumszieetdd.ofcqoNunoacnutrairtteaentmtiploytn)dwuwraaissngm0s.a1ad1me5plmteyo/aLqn.uaalinynstiitsfhiyswasosntd9t9.h.e1ThSbeaasmimpselaeonsf c`hosolaulmrepsclteresadntgaaketedntefsarttom7in2i90th.i3oautri0osnrah1na0gd2e%dmefoafrsonumorme9i1dn.a7lv.atlouFe1so ftih0oeomfabn85ioo2mt.ii0cn%atcloon.9trM8oe.las1s,%uomrefeadsnuvoramelinudaelsv.aflourMeessaafsmouprrlseeasdmtpvalakelesuneatsta9kfeo6nr anto7m2i-nhaolurs ranged from 81.9 to. 105%of nominal and for samples taken at 96-hours, 90.3 to 103% of Summary of analytical chemistry data: concNeonmtirantailont,esmt g|| 0M,e7a2s,uarnedd 9v6a-luheosurast|| coMnceeanntrmateiaosnu,rmedg| Percent nominal | respectively, m; Negative control All <LOQ <L0Q P= T 57 TT am10e7,m11s2,s12w.1 [55si} 15 | 263 10978,.421391,,426037 20 or $53,560,883 een 53iG8ab3iotie) | Not analIyszsed, 150, 171699 9928 Control response: sasfuctory o ENVIMRDQOO)I FINAL Biological observationsafter 96-hour: conmMceeeansaiurnraetdon, my || Mofecaenlinspuemrbemir | InPheDierbnciseiontnytvia || ATrnePkaeirbUcineidnoetn.rvtihae|| `Came IGPnreboritcbheinRitavetine NegativEeEconor|EZ0Y 00 Taso T | S 5_ 5 tC e 3 | 1| ai 6 EseosCorn |E [ rN p TNs m N s Tp a0 TT ses o % Oonobftstiehcreevaaabltlgieaoecnhs1a:0ngAtehfeserfilna9sc6kesh!oiuncrotsohrofnoeergxamptooirsvpuehroeclotnohigrvyelwwohreenarnnyCoo1mspiagnresedaofa6mgg:trheegraaotguiaopnv,e1occadeoduilntai,otno,Snthuoofreuadtwheesrrefnocne ells appeared cnlrged in the 86 and179mal. resent arp. HrRoeuvoresr.wsiAboliblisiqeturyovteoodffGintrthoehwestrthecsoIovnlheuirtbyiioptnhiawosnee:,rTthiheetee17d9wcmiogtm/haLtglgaeaaltgmmreoenwdtthigwraaonsudpfcwasoumrabusefioesrmTnaldleyodditynei,biBtadseadfcenr 96. concuusions cyTaahrpefipcooturanmsdsiineummhvaepsegdcreoltwuetorhrmoiconuceidenwcisifwnoarstai7t1cee(9c66a7l-7chu4olu)artimoEngCmseatnahdnoddbsy9gB5ry%ow<tc<honrfdtiedeen1mc3e6 (i1m1w5eans1a3l18)f(om6eS.e1leTa3hsetmor:n oSfeiutnrssuoNbfOsatEganCrceegwaawtsaisdoendt,eetoremrcimneiednaebny0b,Dauonlrnagetatdeph.rocrewdeuersen(noptc<ed0.n09atoben4o4he megt/LS. woinlsoi etnhte ceotmrhoorsTxho Submitter 3M Company, Environmental LaboratorPy.O,.Box 33331, St. Pau, Minesot, $5133, USA DATA QUALITY Reliability: Klmisch ranking rREe FERENe CES Ll LL This study was conducted at Wilde Inernronal Li, Eaton, MID at he request ofthe 3M Company Qome er eet LW] Last changeds553.00 = ENVAMRD(2002)17FINAL (RDoabpuhsntiaStmuadgynaR)eport Reference No. 3 - 48-Hour Static Acute Toxicity Test with the Cladoceran TEST SUBSTANCE OIcdetnatnietys:ulfoPnoitcasasciidu,m1.p1e.r2f,l2u.o3r3o.o4c:t4a.n5c,s5u,l6f6o.n1a7t.e8;,3m8a-hyeptaaldseocablleuorreof-e,rrpeodtatsosiuasm sPalFt,OCSASor# 2F7C9955..39.3)(1. RsNteMomrRaerdkausnn:ddecrlTehammeebnfitseatnltsauncbaosnltdyaisnteciseontsescaphrniiwqohuriettseo.tpeostwidneg.r.PSuraimtpyldeetwearsmitnaekdentofbreom903.M49l%otbnyumLbCeArM2S1,7.HSMamRpl,e wVaF.s MMeERTHOOBD TMeestthoydp:e:OSEtaCtiDc a2c0u2teand OPPTS850.1010 G`LePa:r coVmepsleted: Study completed 1999. Report completed 2000 SApneacliyetsi:caDlampohnniitaormiangg:naPFOS measured st 0, 24, 48-hours TSbetiasnttoimsidtaialcpahlprnmoiebdatbhisolodiustr:ycewE:iCtOh,bntvoaanillnuieendseacfarrloicnumtleacrtupelodtl,uarwteihsoenmnuasipionnstsgaiitbnhleeed,cobbmyyppuWrtioeblirdtlsiaofnfestlwIyansrtseeo,mfamtoiCvoEni.anlgStLaeivpdeh.raangEeasmteont,hoMdDo.r PIAd.entUifSicAation of the original brood stock was verified by the Academy ofNatural Siences, Philadelphia, "TTeesstt dcoanpdhintiidonasge at study initiation: <24-hours DDiilluuttiioonn wwaatteerr: c0h.e4m5iustmryf(edruerdinwgeltlhewa4t-ewreek period immediately preceding the tes): AHlakradlnienistsy:: 113725((1261-17366)mmg-/L1gaass7/CC8aaCCL)OO,, pTHo:c: 18308m23g) CCoangdrucattiiviot:y: 33153/133150-315) ymhosiem NaK ratio: 2136.62 HLoiugrhstiinggh:t,Choloourrtosndeark50wiftlhuoare3s0c-emnitnluitgehtst,rainnstietnisointypearipopdr.oximately 359 lux. Photoperiod of 16. 9S1tocmkglaL.nd tIet swsaoslutmiioxnesdprfeoprar~a1t9i.o5n:houArsprpirmiaorrytsotoucske.solAutfitoenr wmaisxipnrge,patrheedpirnidmialruytiosntowcaktewriast tpersotposrotluitoinoanlslyapdpileuatreeddwcilteahrdainldutcioolnorwlaetsse.r to prepare the four additonal test concentations. All Edexpptohsofutreestvesssoellu:tio2n5w0asmL6.4plcans.tic beakers containing 240 mL ofest solution. The approximate NNumubermooffbrdeaplpeihcnaitredss:ptewro.replicate: ten `NWateur cohmefmciosnbtcreynte druartiir nognst:hefisvteudpyl:uas negative control Dissolve8d6o-xy8g9enmpr/aLn.g(eco(n0tro4l8exhpoousrus)re) 9 ENVIMRDOODITFINAL pHrang8e6(-09-.48mhpoulrs)91me exposure) Test t58e25m-58p65 e(0r1acrnomgeany(e0txe4pxoup5soushrrueor)eue)) Element bass:1m19o9r3t.a5l2it20y01a2ndCCi5(mc1momanbptiolilzeaxeipxoopnsousruer)e) Method ofcleulting mean measured concentrations: arithmetic mean RESULTS Nominal concentrations: Negative contol, 12,20, 3, 55,91 mg/L. Ll EMleeamseunrtedvacluoen2c4e0n-th5roa%tuicroonnEsfC.id<e=nLc05e02r,(3111v-,823)0m.ph3l.e5n,i9n1brmacgk.ets) 224ah-cohhuooururEEECCL===C2599311(<mm1p1el-l0.1(()CC1m1.prnoo.tt ccallleabale)) All clement valu4e88s-hbhaosuoerdEEuoCCnrm==ea66n31m((e31.s9s11e)5dm9clmo1n.gcre)n.trations cSprtooambtipitsutatinecaalsyesoviafsliutahateiomonfo:vCiETn.hgeaSEeerpCahgaevnamluTeehtsehoaEdndCo.9b5ian%nodmcioEanflCidpervnaoclbeuaeisbnltweyrevweailtschawlnecoru-leaitcneacdleuwrlheterendpopwlohaetsinoipnossssuiinbnlge ttbhhyee BimrmuocbeiVlietry.t method because thre were ss than wy Coneenuatons wih pana mona Aidgnehat.leyrtmpiiecnacilfnogrmhmeeatnhccooednoclelonitgqruyai:dtiAoncnaholrfyotsmehasteoogsfrassptuhbysstoalwunitctiehonnsmtawhseersestsppesercoftlortroommneesdt,riattcheWdiselaelmtieoesnn'Idnth(eonPttLipoArnosSmu)Lei.ntiupseicnskg pvienerdscipevoinndtsuearlefcioosrvoempreeyrriofcflucmooarmtopoiocnnaenfneotsrslf.fioaTnthaietoenLsOaaGsna(luymszeiedt.docfonNqcuouanrtrSietneattpliytonr)ivwanasgsm4s.aa5md8pemle/10rlul.nistthiyswassodns96..h2 eThSkeohemenosf fooSualmrlpsclretasdntgaaekdetnfessottm2n941ah.to6iuortsnr1haandge0omdfefan6rsooummrie%9ad2l..2vathoe1s1f5r%omo.f5n5o.m5i1nal.1M1e2a%suorfednovmailnaule.s forMseasmmprleesdusaklesa f3o6g Summaryof analytical chemistry data: [|| NCoonmcienncaalioTnesmtel.||| 0es,saen2dd464d-umhpo,eiuclraerdesvpeactisvealy,||ConMceeVanseturaraetid| o|n PNeowmeianraolF Negaive Contal i A100 toig |] --=LOSE 172,181, 2S8.D21A6.214,185 302541.340,36.1313.340 | 1 0 joo] 35[100] TE S05.495,570.630.565.56.4 | 56 02 [ 91 $7.6,102,90.1, 84.4. 887, 92.4. 9 [0 Biological observations ater48hours: Daphnis in the negative contol, the 11 and the 20 mglL 0 ENVAMRD(2002)1 FINAL nstirogetnaestdmoeinnftttshoixasipcsiptteyua.dryeFdwievhreeeaplmetorhrcyteanaltnitdmyo;nrontarolmiiamtymtohwbriaolsuigzohabotsuietornvthewedaesastnto4wt8ie-dthhoautnraosnmyionrteatslhtiectoynn,ecgeianmttmirvoaebtiicloonin.ttryolo,r ovTehrtecelifnfieccaels Cumulative percent mortality: [MeanMeasurNeedgaTteisvteCCoonncternotlrationmgl /L]24o -Hours|458-h1our]s| LITT 1 [oo0 T"00 | 3 00 r ---- 36 91 loT7351 [35100] Control response: satisfactory ECOO NCLUSIONSeeeeet LH cw"oTihntechepnaottr9aa5stsi%oinucmownapfesird3fe3lnucmoergoio.ncttearvnaelsuoffon3a3t-e9148m-gh/oLu.r EThCe 4fo8r-hDoauprhnnioaimmmaogbnialiwzaatsiodnetaenndminnoedotbosebreve6d1 emfgfe/cLt `Submitter: 3M Company. Environmental Laboratory P.O. Box 33331 St. Paul, MN $5133, USA DDAo eTAmQaU@ AgLITY---------------------- Reliability: Klimisch ranking | BRE EFERENCES ee "This study was conducted at Wildife Internationa, Lu. Easton, MD at therequestofthe 3M Company. Oe THER rectile Last changed: 51300 100 ENVIMRD(2002)17FINAL (RCorbaussstostSrteuadvyirRgeinpiocrat) Reference No. 4 - 96-Hour Shell Deposition Test with the Eastern Oyster TEST SUBSTANCE OIcdtenatnietsyu:lfoPnoitcaassciiudm1.p1e.r2t,i2u.o3r3o,o4c.t4a.n5e.su5l.f6o6n.a1t7e.;8:8m8a-yeptaaldseocalbleuorreof-e,rpeodtastosiuamssalPt,OCSASor# 2F79C59.53,9.3()INsRteMomrRaedrakunnsdd;eerTlheamemebntietseatnltsaucnboasnltdyaisnteciseontisescpahrnwiihqouriettseo.tpeoswtidnegr.. PSuraimtpyldeewtaersmtiankeedntfbreom903.M49lo%tbnyumLbOerS,217,HISaMmEpl,e wVaFs. Mt ETHOD eeter TMyepteh:odS:utOicPaTcuSte850.1025 GYLeaPr:cVoemspleted: Study completed 1999. Report completed 2000 SSpuepcpileise:r:CrP.asCsuosmtmrieansviOrygsitniecraCompany, Inc. Balimore, MD, USA suSoshuiennldgledga,risbnmldauilnnltge:plreoPcfrtiirloierc.tgoritnedter.iniCtaiarteionw,arsecteanktelny tdoeproseimtoevdesthheel sahtetlherirmouunndiefdor(mvlenytrtaol)preondducweasarsemmooovteh,d EAnxaploystuicraelpmeornioidt:or9i6n-gh:ouPrFsOS measured at 048,96-hours erSsteatdtiuimcsatttiieocdnalbinymesvthiehslouladlsg:rionSwshpteehlcltrieoglnratooiwfvtehshtoeilnmhigebraiotniwotnshhewlilanhsigbrcioatwliotcnuhldaaitntead.thfeTohrneeegsaahctehilvltergecroaontwtmtreohnlt.dagTtrahoeuwpaCsaisesvatlhveuaaltpueeedrcwefanostr s`nhoDerlumlangleritosywtehsatnadswachsoommuposageredendteoittiodytehneotifcfoynviatrrroiela,antcmeesntugsrionugpsthtehatChhia-dSqausatrateisttiecstallaynsdignBiafrictalnett(<0te.s0,9)rreesdpueccttiivoenlyi.n "LTeset oyns3t3ge.r8at(g2e7:h.8u4n:15k)nomwnm, Pretreatment: None "Test conDdiiltuitoinosn: water: Natural seawaterdilutedto 3 salinity of20% with well water Dillionsawlaitnietry:chemistr2y1 (2d0ur-in2g1t0he &-week period immediately preceding the tes): TpoHc:: <8113o0m8g2L) wmSititoxhcekdsofaomnred~2wte4hsithtoesuorlpsuartptiirocniulopaorrteeupsmaear.taeAtrfiitoaenlr:smPuirxsiipnmegan,rdyepdrsittmohacrrkoyupsgrtheoopcuaktrestdohleuitnisoodnliultauiptoipnoe.narwIetadtcwelaresaartpra9on.pIdomrcgto/ilLoo.nralalenlsdys hadiipglphueetasertdedcwoicntlcheeandrtiralaunttdiioocnnolaowtraaletisnesa.b1l0Deupwerietfphoarttehhiesthrmeealafttoieuvreliaydaldpoipwtroosnxaiolmlatteuesltbyoci3of.nl3cPmeiFnett/OrLySa.tiionnsn.atuArlall essetawastoelurt,itohnes dE`eCxpoptnohcseaunprtpreraotvxeiisosmneasltsde:olsySi22n11gprcoamlt.yee:EtOahnyclhceencehaamqbuaerriawacosnctoanitniinnugoaupsplryosxtiimrartedeltyo 4c0irLcuolfatteestthesoSluutpipolne;mewnattaelr 02 ENVIMRDGORTENAL 1dFale)adueiTnndgiEu:gsApl,rgoalvecaendlle1)Tiuhcaplupadseseriamsisrre,dlonaoncaumSitnlgetao1renand.foCvhaicivceeasisp. anrd bocsh NNNuuummmbbbeeerrrooofffcroeoypntlceiecrnastterpsae:trinroeenp:lifcacte:pviesrygis conrol Water cDhimsoilrvyGd6douxryTignemgnsr.hans(geanyo:l9xhpouors:) DH ran66g150-7..719chooe.n0emxpeosturpe)or) est tem7p6er7a8t1urCeroamnge (0x-p5onorue)r 22.2-22.3C (control exposure) Methodofcalculating m2e1a.n8-m22e.s7sCe(d3.c0omngse/mLteexmptoosnusr:e) metic mes resus ENNiloeemamiseninartledvcaocnlocune:cnet(nr5tar5ta0itoicnoson:snNde<gLea0tn0iv.te0ec3orn6ii0ol4,0g,1h2e1,n210i1,n 5b3r.3a,0c5em5se,.. mg. Alone valuesbased 9Sonh6e0euianNEOCmaeKsC=s-3e0d1m9cmoggn.c(eCr1nnsok clus cEStoaCtniesatmilacaenlosxnacuoaltdionontofbeshsellgerodwth:due 10 sles shel ron iii a1 the ighes tse rdiaegesthtpotcnipsieenrciffonorMhmeepatnheccoloedsnooclTenonigoryoda:cinoAcnenhuorslfotymihaosetno6oegfraswSpathusybseaowdlcia.teohniNsnmohawseessrcettppeesworlcufwtaioosrmn,aaseeWdsieatfdeonceetQisonnedns(rHtaPOnLpnCrt)oe.Lmip.ihWwphiaenokygF Tfciaanokndeligrieladitaol4amhsoosumter0rsni0cai1cni0o5mdn2po5n4aFeondftmsom.1reT51hai0e-d4L1OvGa5lu(eomsfifntooomrfa3q.5 u1M4sa4a%sneodfwsevasmi0io1n1l5fMomseg.sitckaibsoevenaodrmSsooormniosnl Summary ofante chemisey dat: ConcNeontmcaatrioTne,smtelt || MVaelsuseSseh1do0p,u3l,8oawnsd || CoNnceenwtreatsiorn.smdg?| FesenorNom | e2r 0R3e5E p,e0a35lC .m05g37|-- 034 - 0.295100 410% TT 20 0/622, 0.633. 0.299, 0.40 20 n ENVAMRD(002)17FINAL 33 ed 136E ,115,092E 4, 0878, 1.58, 1.72 3 55 222424,,215435,,20092,7 5 oT 37343,935,73,48139091.2,19 30 3 3 repicates analyzed at time 0 aBpipoleoagriecdalnoorbmsaelrvaantdiohenasltahftyetrhr9o6u-ghhoouurts:thOeyesxtpeorssuirne tpheerionde:gative control and all PFOS treatment groups Shell deposition and shel growth Inhibition at test terminations ConMceeantnraMteiaosnu,redmg/l. Negative Control 036 Ty ea 31%9 Mortalityofcontrols: None Shel Depositmionn Mea+n SD| Percent inGhriobwittihon in Shell 267-05 - 2.500933 T/T d25r15o09s19 12.9113 2300.589041. T EL 2T1 CONCLUSIONS reece ess erese ete sm------ hm"Tgoh/ueLr.phootthaeesffsheiicugtmhceospntecrcefonlntucrreaontoticrotanatnwicoasnsult1ef.so9tnemadgte/aLn9.d6-thhoeuprraEcCt.icqalfolrimtihteoEfassotuebrilOtyystienruwnafisltdeerteedrsmeianweadtetro. bTehe> 936..0 Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133, USA DmDApTCA OQUMALNIYTY. Reliability: Klimisch rankin=g | REFERENCES "This stwauscondducyted at Wildife Intemational Ld, Easton, MD at the request ofthe 3M Company. e oTHER eee l Last changed: 5/3100 108 ENVAMRD(2002)17FINAL RMuosbsueslt(SUtnuidoycoRmeppolratmaRtesf)erence No. - 96-Hour Static Acute Toxicity Test with the Freshwater TEST SUBSTANCE OMcdetnatnietsyu:lfoPnoitcasasciidu,m1,p1e.r2f,l2u.o3r.o3o.c4t.a4n.c5s.u5l.f6o6n,a1t7e.;8,8m-ahyeptalasdoecableuorreof-e,rrpeodtatsosiuasm saPlFt,OCSASor# 2F7C9955..39-3)(1RsNteMomrRaerdkausnn:ddeerlTehammeebntiteeasntltsaucnboasnltdyaisntecisoeintsescpharnwiihqoiurtetseo.ptoeswtdinegr..PSuarimtpyldeetwearsmitnaekdentbfer9o0m.34M9lo%tbnyuLmOMb2Se1,7r.TSHaHmMpRl.ewVaFs MMOB.ETHOD FPMiresotht,heocddt:ciountTeAhgTeoesnsictcuyidyySewTreaisests;c8ao5nn0ddu--ActSEecTdoMluosgiSintcgaaanldaEprfrdfoetcotc7so2l9Gu.bia8ds8ee,ldiSnoetnsa,npdrOaoPrcdPedTGuSurieNsdeuomuftbolreirnCeo8dn5di0un.c1Ut0.iS7n.5gE5TnovOxiiErcoCinsDmeTn2et0sa3tls Twietsht Ftiyspeh:es,SeMmaic.srtoaitnivcerRteenberwaatles and Amphibians GYLePa:r coYmepsleted: Study completed 1999. Report completed 2000 SAStpnaeatcliiysettsii:ccaalUlnmmieootnhciotodonsr:pinlgLa;CmTsaessvtalsuuebsstcaalnccuelactoendc,enwthreatniopnosssmibelaes,ubreydprboybiLtCanMaSlysaits0,,m4o8v,i9n6g-haouuerrsage method or "UbTiSenAso.tmoiarClgaraponrloiibsnamabisclooilutlryeccweti:etdhObfniroaonim-nlcithndeeafwrriloidmn,tCeraproollaitniaonBuisolionggictahlecSoumpppultyerCosmofptawnayre,oBfurCl.iFn.gtSotne,phNaonr,th Caroling, "TTeesstt oCorngdaintiisomnasge a study initiation: Unknown DDiilluuttiioonn wwaatteerr: c0h.e4m5iustmryfi(ldteurreidnwgeltlhewa&t-ewreek period immediately preceding the est): AHlakradlnineistsy:: 117246 ((1710-1275)0mmg-/L13/ a5s3CC2aalCC)OO,, TpoH:c: <3313o1m8g5L) CCoandMucgtirvaititoy:: 3325113(.3510-330) hos/em Na ratio: 2136.62 hLoiugrhstilniggh:t,Co8l-ohrotuornscdtark50wiftlhuoare3s0c-emnitnluitgehtts,rainnstietnisointpyearpiopdroximately 369 ux. Photoperiod of 16- S9w1taosmcepk/rLoa.pnodrtIttieowsntaasslolmlyuidtxiieloduntsefdoprrweaipptahprradtoiilxouitnmi:oaAntewp2alrt4yiemrhaortuoyrpsstrpoercpikaorrseotltouhtueisof.nouwrAafastdepdrirtemipioaxnriaenldg,fienshtdeiclopunrtciieomnnatrrwyaattsietoroncaskt AElxlpoessturseoluvtesisoenlss:ap2pe5arlietdercpleoalryeatnhdylceolnoerlaesqsu.aria containing "The approximate depthoftestsolutionwas 23.2 cm. approianatly 20 Loftest solution, NNuummbbeerr ooff rteepsliocratgeasn:istwmos. per replicate: ten `NWuamtebrecrohefmicsotncreyntdruartiinognst:hefisvteudpy: laneugatsive control 10s ENVAMRD002)I FINAL Dissolve5d8o8xy.g5enmgr/aLng(eco(n0tr-o9l6exhopuorss)u:r) PH rang5e0(-0-8966mhgo/uLrs:(19 mg/L exposure) 7509--8845 ((7c9onmtgo/lLeexxppoossuurer)e) "Test tem2p1e4r-at2u1rerCan(gceon(t0ol9e6xphoosurusr)e:) cEolnesmiedenrtedBasdieasd:.2Mo1r8Tthale-i2ty3n..u7mbMCuesr(s7eo9lfmsignw/diLitvhiedoxuppaeolsnsusrehex)ehilblitainndgncolinriecaslposnidginnsgotfo gteonxitclietyproorddainbg woe)re `beMheatvhioodrofalscoalwceurleateivnaglumateead.n measured concentrations: arithmetic mean RESULTS M`Neoamsiunraledcocnocnecnetnrtartaitoinosn:s:<<LL00QQ,,575.,3,111,22,32,0,4461,,9179mgm/LL Element value: 9(69-5h%oucronLfidCe=nce$9inmteryval(5ig1i-6v8enmgi/nLb)rackets) cpSrtooambtpiiutsttaiencraallsyosefivtsa,wtautraheteioomfno:CvEiT.nhgeSatevLpehrCaang.evamlueetshoadndor9b5i%nocmoinaflidpreonbcaebiilnitteyrvwailtshwneorne-cianlceurlaitnetderpwohleatniopnoswsiibnle tbhye dhAeintgaehlrymtpiiencriafnlogrmtmehatenhccooednoclelonitqgruyia:dtiAocnnaholrfyotsmheaestoeogsfrtatespsuhtbysstoalwnuitctiehonnsmtawhseesrteessptpeserocfltourtroimmoeendtsr,iattchWedisealtemdcetliaonIndntm(eomHsaPttLipoOnrMaoSlm)iLn.de.ntWuphseieannkg pirenerdscipevoinndtsuearleficosorovmpeeerrryifcloucfoormompoaoctntreainnxctssfu.olrftoTinfhiaectLaetOiwoQnass(amnusaieltdy.zoefdqNuoacnotnaicttutarterimeopnntt)lwywaassd0u.r1m1ia5ndgmegsa1/m0pLlqeu.iannattnihaiflsyyssotiunsdy.twhaeTshbea9s4mi.se7%ao.nf tv`Saaaklmeupnelsaetfs9o6rcohslaolmuepcrltseedsratantagketeednstfatrion4mi8ti8ah8to.iu5orn0shra1adngmee3doafsfur0nroomemdi8n%1va.al2l.utes98fr.o9m% o7f3n.o7mi%natol.96M.e0a%suorfednovmailnuaels.foMresaasmuprleeds Summary ofanalytical chemistry data: |[CNonocmenitnraatiloTne,smtg/L|| M4e8a,saunrded96du-phloiucrasterdevsapelcuteivsel0y,,|| MCeonacnenMteraastiuorne,d || PNeormceinntalof Negative Control | Almlg<L0Q L "Log 57 547.493, 518,570,330 536 53 %- " 1 23 190.168, 187,187,229.224 | 20 | 109 87 o4t6 631920,.470467,.378.113,.3797.65,,848822,,845075] | "7a01 | | wgo m]| ttBorineoiacltiotmgyei.nctaFslivaoepbpspeeearrrcveeandttihomenoasrlttaahlfyittarynwd9a6ns-ohorobmusasel:rvtehMdruosaustge9hl6os-uhtionuthrtesheitennstetghaewti4it1vhemncgoo/nmLtortotrlaelatithtmeyeno5tr3,aonvd1e2r9t0acn%ldinmitochraetla2lsi0itgymnsgw/ooLsf observed inthe 79 mg/L treatment. No abnormal behavior was noted in hese concentrations. 106 ENVAMRD(2002)17FINAL Cumulative percent mortality: [MeCaonnceMenatsruarteimdoTgnest | 24 Flours[*m] 72 Ho[us 57 -- T oo yr 7 30 Tw T 50 w Control response: Satisfactory CONCLUSIONS cd"oTehnteceerpnmotitrnaaestdsioitnuomwbapeesr52fl90umomgrg/o/oLc.Ltawnietshulafo9na5t%e 9c6o-nhfoiudrenLceCinftoerrvtahleofFreSs1h-w6a8temrg/MLu.sselT,heUn9i6o-hcooumrplnaomamtourtsalwiatsy `Submitter:3MCorporation, Environmental Laboratory P.O. Box 33331 St. Paul, MN 55133, USA DATA QUALITY Reliability: Klimisch ranking |. REFERENCES "This study was conducted at Wildife Internationa, Lu. Easton, MID at the request ofthe 3M Company. oTHER Last changed: 51300 07 ENVAMRD(002)I FINAL Robust Study Report Reference No. 6 - Activated Sludge, Respiration Inhibition Test TEST SUBSTANCE _ OHcdteannteitsyu:lfoPnoitcaascsiidu,m1.p1e.r2f.l2u.o3r.o3o4c.t4an5e.s5u,l6f6o.n7a7i.e8;,8m5-ayhepatlasdoecabfeluorreof-e,rpeodtas10siuams saPltFCOASSo#r27F9C59.53,9.3)(I. RsPoeFrm-eaNdrMkuRsn:daenTrdhaeemlebesimteennsttuabclsotnaadnniactleiyosinsessawpterhciihontioqpuoetwesdsteirng.. SaPmuprilteywdaestetramkiennedfr0omb3eM90lo.t4n9u%mbbeyrL21C7/.MS,Sam`pLlIeMwEa.s METHOD. eeeee ee MTeestthotdyp:e:OSEtaCtDic 2a0cu9te YeGLaPr:CVoemspleted: Study completed 1999. Report completed 2000 TASetnasatltiysottiriccgaaallnmimesotnmhisotodousrri:cnePg.r:oAbDciittsisavnoaaltlveyedsdissolxuudysgigenegnctochloelneccceotnemtdpraufttrieoornmss.tohfetwParroesopfecCt.EB.ayScWpahsatne,water Treatment Facil, Grasonville, Maryland, USA. "Test conDdiiltuitoinosn water: NANOpuret: Syntheti1lciSteerwamguen:icipal water 1161.00..p8empeattonexetract 0370ggNuraeCal 0042..2 CMagCSl O21T,H0O 28g KiHPO, dNRieAcfNhelOroeprnoucprheeenaanlw,datwteaerss,tptrhseeonplaubrtierodnobypurdtgeipshastrohtalevtpiinogn:5oo0Af0inmnscgtiopciitnken1st0olmpurtLeicoionpfitoIafNtitoNhnaewOirHte,hfedr|ieNlnuHcte;eSd0s,utob,s3at0anndmcLed.,ilwu3it.teh5d m1P0eFtO1hSoLdwwawisatshaddNdeAeeNdmOeddpirueacrptpelrydoptroiwataettseetbra.vsesesTdeholsenrpattHhheeoorfbtshteharenvervdeoflseuormleuenbtcirleiictsyoalodudftitithoienontweaotsfsumbsetsaotscauknrceseodliunttiowoanbt.eer7T.h1i5s tTreasntsfveersesdelisn:toM3i0x0tumrLesBiwoecrheempirceaplaOrexdygaenndDaeemraatnedd (iBnOD50)0bomLt.sEdenmeyer flasks and then TNeummpbeerarotufrceo:nc1e9n-t2r1atCions: 7 plu3s reference controls and2 Blank controls "ETloetamlenStusBpaesinsd:edReSsopliirdastiaonndipnHhfoir sabluiddegtteeoirnmiodnaenyd obftyedsitsisnogl:ve4d35ox0ymgge/nLcoanncden7t.ra8t7iorens.pectively. oM5fe0sto0hniodcdat1iRo0en0m.0amrTkghse/r:LefSoitnroecN,kAdNsiorOlePucttuiworenesiogfhwtaPatRdcOdrSitciotonhntaatwiawnseerdeemteppslrteompyaaetrederditaoaltadtnhmoaimtniiwnsaatslercnooPtnFciOennsStorltauotttiihooenntoeafsfattpsepyr2s0to-eimmmiantuetlcys 108 ENVIMRDQO)I FINAL oiTvetsehtrahmiepnetrSuiroedEsowfAeurpes0lpurde1gp0ea-rwmeaidnsuatisr1Se-mhionuurtse. tAefvra3-ahnodrscraaftceodntuacntlttnheecoAntaRctstiegoifotnhe weet snubstyance A RESULTS 2N7o1m7i9n0a5lmceolnc.entetsrtamtaitoenrsi:alTswoloutiboansr.k conrol, thre reference substan contls, 090, 2, 90, 27, %, cStoamtpiusttiecralsAonaalyrseosf:CEE.CStveaplhuaens.wAenrEcaClcuvlaatleude coorutdheorrebfeerelncuetmaetedriafolrbhy ptrobist apnsoiysnis using the rnAentcaroiarttdiiecdoanelarlMyeLtdh1.o0dsuoeslcioongngyd:sfYAovSa1l0yMsomiidsneoufltDeSsOOouBcnDoinlssseorhlievreaddiiosOnsnsoyligvneedanloMxteyesegtre.sn dorDooipspnseodlwvbeeredelaoppetrfo3onrmmse.daattiWsilwodne: RespirationRates and een nibiions | Treatment RemsgpirOLationhfouarte Percent Takbiion a [.C5ointcrloolrophanal Sg T iSHITT E SAAN wNAry 25 [7S75 5iScchholrooopphheenncoil 5105 mg Test tance 090 mpl fm isis o4F So 1 [Test substance 27 mgt [TTeesstt ssuubbssttaannccee 29.70mg Ei) 35 M-- RKiS-- e 79 TTeesstt ssuubbssttaannccee 29701 mgmg (est ubsancesome 1MY 32477SRESN3 E gTRAY Control response saisfciry "cThoexctueussbisotnansce cXhbied 3 substance concentation of 905 mgaLxi.mumThoe ErCy(rCsperattioofn Ti8%bupnon risehsepiorns.aE3xpcotman sn Solubilityofth est substance Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.Paul,Minnesota, 55133, USA a DATArQUAy LITY Reliability: Kimisch rankin|g REFERENCES This study was conducted t Wide Intrnrionl, Ld. Eaton, MDa the request ofthe 3M Company. 100 ENVAMRD(002)17FINAL e oTHER nr ee mea re tor He. Last changed: 5/3100 no ENVIMRD(002)17FINAL (RMosbiudsotpSstiusdbyahRieap)ort Reference No.7- 96-Hour Static Acute Toxicity Test with the Saltwater Mysid TTEeST SUBSTANCE eee OWcetntaintcys:ulfoPnoitcasasciidu,m11p,e2r,f2l.u3o.r3o.o4c:t4a.n5e.s5u.l6f6o.1n.a7t.e8;,8m8a-hyeptaaldseocafbleuorreof-e,rrpeodtatsosiausm saPlFt,OCSASor#2F7C9955-.39.3)(1- sRteomraedrkusn;derThaembtiesetnstubcsontdaintcieonissapwrihoirtetopoewstdienrg.. 19F-NMR and elemental analyses techniques. SPuarmiptlyedweatesrtmaikneend ftroombe39M0.lo4t9u%mbbyerLC2/1M7.S,SaImpHlHeMwRas METHOD ee eeell el TMyepteh:odS:utOiPcPacTutSe 850.1035 `GeLaPr: coYmepsleted: Study completed 199. Report completed 2000 A`SSnpueapclpiyletisie:rc:alMyImsnoi-ndhiootpuossrieisncgubl:athuPireaFsO,SWimkedaiesuInratetem0ad,ti4o5n,al9,6-Lhiodu,rsEaston, MD, USA ExbStipanotoismsiutairlcealpprmeoebratibhoiodld:ist:9y6w-LihtCohusrnsvoanlluienseacarlicnutleartepdo,lawtihoennuspionssgitbhlee,cboymppurtoebirtsaonfatlwyasries,omfovi.En. gStaevpehraange method or "PTreesttrfeiasthmaegnet:: =N2o4n-ehoursold "Test ConDDdiiilltuuittoiinoonsn:wwaatteerr:chNeamtiusrtarlyse(aduwraitnegrtdhielu4t-ewdeteok2pe0r%iodwiitmhmewdelilatwealtyerp,r0ec.e4d5ipnmg tfhieletre.): pSaHl:inity: 20 2020) % 828182) Toc: <10mgL wHSoatutorecsrkpr0aionrpdr(et0peasutrsees.otlhueAtfilfoeonurrmpriaedxdpiiantrgia,otnipaorlni:mtaesrPtyrcisomtnaocrceykntsrsoatltouictokinospn.rewpaaAslrlepdrtoeapsttor8St.oi2lounmtaiglol/nyLsdiaalpnupdteeadmriewxidetdhcldefiaolrrut~ai2no2dn Ccoolnocrelenstsr.ations dosing rate: Once. wEStaxatpbeoirlsiduterypetohftveahspsepelrtso:exsit2mcaLhteepmloiylcy6ae.lt6hscyomll.eutnieonasq:uaErxitarecmonetlayisnianbgleapproximately 1000mL of test solution; NNumubermooffbrmeypsleiicdastrepse:rtrweoplicate: ten FNeuemdibneg:rofLicvoencberninterasthiroinmsp:niavupelipilduaasilynegative control WatercDihssoelvemddouixryigsnegntrthanrsgteuyd(y0: 96 hours) 6658--7734mmpg/lL ((c5o.n4tmroellexpeoxspuorseu)re) PHrange (0-96 hours): m ENVAMRDQOODITFINAL "Test emp58e11r2-at58u22re((rc5oa4nnmtgopell(e1xepxop9sousrhueor)uer): 242 254C (control exposure) Me2to3.f8cha-l2c4uo.l5atdiCng(m5.e4amng/mLeaesxuproesudrceo)ncentration: aitmetic mean y RESULTS ee g EMNleoeammsienunartledvcaoclnoucneecnet(nrt9ar5tai%toincoson:sn:fiNde<egnLact0ei0v,te0rc.5ao7nl,tl11g,1i.1v1,e9r1,8i5,n0b,3a.$c04k,em4rp9sl5,..2 mpl. Th242o-hhououurrrLLLCCC.s= ==4~>454.(4 m3mp.Ll.6C(6m1C.e12lnn)oo.tt ecaxluclublalbel)e Al clement alues basedSohnomeuanLCme=asu3re6d co(n3c0e4.n0t)amtipoln.s mdStoeartail1s0itittcyhaelatea7vc2aklauonandti9ao6nnhaoodfuermqso.urattaelictoyn:ceLnCr.uivoanl-ueesspcoonusde pnaotttebre.caTlhleprdobfiotrm2e4thaondd 4w5o-shosuernsdoaf epxposnure dHAeintgaehlrymtpiiencriafnlogrmtmehtaehncocodenocleolingqtyuis:dt.iAocnnhorfsotmehaetotogefsrttaespsuhbysstoawlniactoehnisnmtawshsee.restppeesrcfotoiroomnemde,tsntthWedisedtaeimcfetieaonn'nem(moHasPttLopOnrMolSm)iLn.ie.ntWuphsaienkeg pirenerdscipevoinndtsuearlefcioorsvoempreeyrriofcflcomonmacptoocinaennfetorst.siufliTfcaohtneiaotLnesOawQnaas(liyuziseetddo.cfoqnuNcauonrtraeanrttiloyen)duwwraaissn0sm-aa1md5pelme/1a0ll.Qynusiihsnsswassoany97,h4.eThSpeaucehmnsof Hoscuoarlmepsclteresadntgaaektdeftneosattm4n38t5ah.to5iuro0snr7ah1n.dg1e%dmeoffaosnumorm4ei3dn.a5vl1a0lue7s1.f0r%omof$n2o.m4in1a0l.7M0c.asrofcdnoamlionaslfoMesasmepwleeds oveasne t0r Summaryof analytical chemistry data: [N|CoonmceinntraatlionT,esgt L|Measu9r6e-dhdourus rpespevlctaiiveslyac,tma,p4L8t, aend|VCiocnacreMnitsrsuarteidonPerensofNowa] ENeEgaive Contal rrA10o0 ss ome L [T3o0 I 19 1r9e2,a19b6i19e2,t19w1,iLo1n9s1 | |L 15a |v] ] --i SSSA Sessass |se] ol mBieoalsougriecadlcoonbcseenrtvraattiioonnss)aaftemre96n-hogurros:upsMyapspiedariendtnhornmeaglatainvdehceoantlrtohly,daunrdintgheh0e.5t7etand 1.1 mg/L (mean n ENVIMRD(2002)1 FINAL Cumulative percent mortality: [MCeoancnentMreataisoun,rd Tmegs/tL |2hours [own wa Seto | NegativeConwol | 0 |0 057 Tv 0 me LC LL 19 T 7Te re a r [ 30 ANESNI SO" | 54 [os TsTe 1 95] Mortalityofcontrols: None CONCLUSIONS w"LTihtieh mp9o,t5as%sicuomnfpeirdfelnucoeroinotcetravnaelosuflf3o.n0ate49.66-mhgo/uLr. LTChef9or6-shaolutwranteor mmoyrtsaildistywaansddNetOerEmCinceodncteonbtera3t.i6onmgw/aLs `Submitter: 3M Company, Environmental Laboratory P.0, Box 33331 St. Paul, MN 55133, USA ODY ATA QUALITY ee el Reliability: Klimisch rankin=g | MReEeFREROENNCCEESS "This study was conducted at Wildie Intemational Li., Easton, MD at the request of the 3M Company. ooTHR ER Last changed: 5/300 ns ENVAMRD(2002)17FINAL (RPoibmuespthaSlteusdpyroRmeeploarst) Reference No. 8 - Early Life-Stage Toxicity Test with the Fathead Minnow eTTESTSSUUBBSSTTAANNCCEE OIcdetnatnietsy:ulfoPnoitcasasciidu,m1,p1e.2r2f.l3i.o34r.o4o.c5t,5a.n6c.s6.u7l7f.o8n,8ate; m8ahyepatlasdoecabfeluroerfoe,rpeodtatsosiausm saPlRt,OC S or#A 2F7C9955.S ,39.3)(1NsRteMomrRaedrkausnn;ddeTerlhaemmeebnitteeasntltsacnoaunldyisbteisoistnesscpthrwniihoaqirutteenosptoecwsdtieenrg..PuSraitmypldeetwearsmitnaekdetnobfreom930.M49l%otbnyuLmbOerS2,17T,SLaHmMpRl,e w"aF-s MwETHeOD, -- MTeypteh:oFdl:owO-EthCrDou2g1h0c,hOroPnPiTcS 850.1400 YGLePa:r coYmepsleted: Study completed 1999. Report completed 2000 SSpuepcpileise:r:PimIne-phhoaulseescpulrtoumreesl,aWsildiie Intemational, Lid., Easton, MD, USA EAnxaploystuircaelpmeroinoidt:or4i7ndga:yPsFOS measured on days 0, 4,7, 14, 21, 2, 35,42, and 47 AtStrlaeltaictsomtenintctianlguromoueustp-hsvoatdrhsai:tabseDidshactraetoawe-esvrawaetriiesaevtbaillceudaaldtyaetdsaifgwonriefrnieocraanmtnaaldliiytfzyfeeurdseinuncsgein(Sgh0a.2p0ixr3o)-2Wficrlooknm'tstihntegesentnecgayantditvafebolrceoshnottmrooogliedgnerneotiuitfpyy bofetvwaereinantcreeuastimnegntBaanrdtlceotntsrtoels.meaAnnsa.lysisof variance and Dunnett's testwereused to evaluate differences "PTreesttrfeiasthmaegnet::eNggosne< 24-hours old at test ntiation Test ConDdiiltuitoinosn water: 0.45 im filtered well water Dilution Hwaartdenrescsh:emistr1y26(d(ur1ing2th4em4g--/wL1eeask2Cpea8rCi)oOd;immediately preceding the test): pAlhkalinity: 18722((812473)0m-g/L1as7C2aC)O, CTooncdu:ctivity: 3<2L1o(3m1g5-L330) pbosiem pmSritoxopceokdrtauinnotndialltlaeylsttdseisotlluusttueibdosntawpnircteehpadridasitlsiuootlnivo:endPprwriiaomtraerrtyo utssoteo.cpkrAepfprlaeerpreamriefxdiivnieng,daitdlhdueittipioronniamwlaartyesrtsotactok.c8k5s.so4ollumuttgiio/onLns.waanasd1 atcootnefocoefunr6tr.da0atyimsoLnd/soumrfiinn4ug4t.te2h),e2wt2he.set1.r,e1Tt1.hh0ee,ys5wi.ex52rs,etaomncikdxse2w.de7w6riemtghi/ndLjie.lcuttSeidtoonncktwaostethroe(dlaitlauuwtaeettrremeiopixrfoienp1gna16rcmsehbdammeibvneeurrystet()hartet3eo SaFtclahobiiwelvitethyrthooefutgdhheesirartteeed:tecAshtpepcmroioncxcaienlmtarstaoetlliuotyinossn,isx:voElxutmreemaedldyitsiiobnlseoftet water every 24-hours aaEppxppprrooosxxuiirmmaeatteevellsyyse5ls01:7mLcmm.gilnasdsiEaammqbeutraeryiroawifitinlhceud4b2aw5tiitwohnmapcnpuyrplososnimwsetcrereeleync7omnLesosthfrutcaettsettdacshoelfdurtoitmoontghwleiatsbhsoatctdyoelmpitnwhdietrohsf na ENVAM/RD002)17FINAL saitlaicchoneed s0ealarnot.ckeTrhaermcwuipsthwaereecispursopceantdiendg Numofbrepleicatres: four minattihoen woafatperprcooxliummnatoefl2cyacphnSL ghss aquarium and NNuummbbeerroofffciosnhcepnetrrarteiploincsa:te:sixtwpelnusty negative control dfFeaedyesdfionrgat:hrlLeoiavuseg44bh08riphnooesutsr-hshrapitrcmihpo,rnfateuodplti3h.etitmeeFrsemddian3aitltiyiomnooensfwpeteeertkddataoyyasdlualrnoiwdngf2ottrhiemgeufstirdsctalie7alrdyaaonycnsewpeporesiktoehrnad1t0csh.w,eiNgOohntt mWeaatseurrecmheenmtiss.try during the study: Dissolve7d.o6x8y.g2enmgra/nLg(eco(n0tro4l7exdpayoss)u:re) PH ran7ge.(608247mgda/yls): (1.2 mg/L exposure) 8800--8844 ((1c2ontmrgo/lLexepxopsousruer)e) Test tem2p4e.r4a-t2u4r.e7raCng(eco(n0tr-ol4e7xdpaoyssu):re) `Methodof calculating m2e43a-n24m7eCasuredc(o12nmcenLtraetxipoonssu:re)arithmetic mean RESULTS ; NMoemaisnuarledcocnocnecnetnrtartaitoinosn:s:Ne<gaLtOiQv,e0c.o1n5t,r0o.l30,0.1,30,6002,9,10.2.,5274,,41..16, 2m3g,/L4.6 mg/L Element value: 452--ddaayyhpaotschtaebhialticthysNurOvEivCal=N4.6 O m=gl0LE .30mC g/L Alclement values base44d22--oddnaamyyeppaoonsstt-m-hheaaattsccuhhrsgeurdrovcwiotvnhacleNnLtOrOaEEtCiCo=n=s00..3600mmgg//LL dSHitifagfthieesrrteintcchaealnbe0ev.ta6wl0eueamntgit/ohLne wnoaefgsamntooirtvteaelcvioatnlytu:raotlTehdaenddusettahtteioshtaiicgsahilegsndtiiffcifoceanrncetenncetferfaeftcotiroogntnreosswuttrevhidvwaaatl.scsoNnecoeonstafroatrtiihsoacntacshlaeybqiulsiiatglyn.itfoicaanndt dAheintgaehirymtipineciarnlfgomrtemhtaehncocodenocleolnigqtyuri:adt.iAoncnaholrfyotsmheaestotoegfsrtatseptuhybssoltwuaittnihoninsmtawhsesreesstppeserocfltourtroimmoeendtsr,iattch WidsleadtlemicefteiaonnIdntm(eomHsaPttLiOpoArnMoalmSi)Ln.iedn.tWuphseiesnnkg rmienesdaipnvoindspueearlcfeoinrstopmreeerrcfiolcvueocrroyomopcootfnaenmneattssr.uilxfo"fnToahretteiLfiwOcaaQtsio(nulssiemdiat.naolfyNzoqeudaantcttoitneacmtupirtornew)natwslaysmda0ud.re0i4n3gto8sqmaumgap/nlLteifiaynnatolhniyssitsshteudwyba.asskT10ho2ef Smeaamspulreesdcvoalllueectsedfraotm ranged from 95.20 11% p9r5e.t5estto r1a1n4g%edoffrnoommin9a1l..4 of nominal, tMoea1s0u5r%edofvalnuoemsinaflor. samSpalmepsletsakaetn taetsttesnt itterimoinnathiaodn ns ENV/MRDQO0)I FINAL `Summaryofanalytical chemistry data: Ln De es || [N|omCoincnenatlratTieosnt, | my M7e,a1s4u,r2e1d,2d6u,pl3i5c,a4te2,vaalnudes47at0Da,y4s, respectively, mg/L MCeonacnenMteraastiuorne,d| Perocfent mg [Nominal NegatiOve14Connell (147,0.160,__0.A141l,i0.<14i0,o0q.15%, 0.13, 0.13[ , oT 01.113557,.00..115630,,00..114437,,00..116505, 0.158,0.179,0.173, [OB 0.0226867.,00320777,.00321750,0,.03.4238,9.0304215,2,0031319.60,.3023655., 103 |[037 0p3s197.013,130,502796.,0207,66015997,,00,6.863,50953,, | 00..557355..00.567068,, 00..538405,0,.60.35943, 0.617, 0.646, 0.644, [I Ua L120, 125,113, 125, 105, 110, 19, iz II = [0 PDay2? 1,22462,2475,2,38,2fish5all2dea,dat Day 567 ,4404.,79,44.476,94.7,6, sh lldeadat Er [TM] ww| BiologicaHlatOcbhsienrgvastuicocnesss and timeto hatch All viable fathead minnow embryos hatchedon Da4yor 5. trTehaetrmeewnetrgeronuopasp.parent differences between the time to hatch n th negative control and the PFOS. `t`oSGxurirocvwiittvhya:.l:FiFAsishlhwihesixhcphossuderidvdintvooitnPgsFutrOovSievsaettgtceeonrnemcrieannlatltryiaoatnpipoaenpasporeefadret0o.d1bn5eoorsrmw0ai.lm3mw0iitnmhg nLeroraotfvioercratl4l3syipdgrnasiysooftpoossduteblahetaththcahl `schoomwpeardisnono tsoatthiesinceaglaltyivesicgonnitfriocla.nt reduction in total length, wet weight or dry. weight in Hatchability ||MeCoannceMnetrsastuiornd|NumEbgegsrof| HNautmchbeedr,|| HSautcbheedr,|| HNautmcbheedr,|| NuTmorble|r| HPaetrccheinntg |[__NegatimvegtComrol||Exp8o0sed T|=D0ay31|2Du0yd ||3Da4ys|| H7atcahe| d| Suc0c3ess | IF 0.15 03 [80 ToTis[58 1 7% 1 05 | so Thao | 88T7T 100 | [os 12 50 80 [28 Taw T71 9s | [72 Tw T 74 | 03 | 24 46 Ts 80 TT 70 T o 140 T6 eT [ 1 37 70 5 || os o 16 ENVAMRD(2002)17FINAL Larval Survival [NCoinceentrnaMteiosns, edmg ~T PerceDntaySaa2rva Negative Control Toe [#8| I 03 AE I 06 12 ==" 24 46 I 30 Growth ConNceeantnraMteiaosnu,rmegd/L|| NSuurmvibvienorgf||TMotealaLnenSgDt,h||MWeeatnWe+iSgDh,t|~[Dry WeSiDg.hmtgM.ean 7 Larvae mm mg Negative Control 0.15 [60 7226656++00722018 |11605 +3.18 0 ||+3329 3534+01 1920000| 030 0.60 0 1674119 3424270 I 12 24 [8T267v202[185+338| 354+666 | A USE EAA ANE - ] LL 3 Tm ir O CONCLUO SIONS eee eee `dFraaoytwshtehpa.odstTm-hhiaentncmohowsssthoseewxnepsdoistnevodesettoantdippsootiticanastlsliyisnuitmghnpiiefsritfculaudnoytrworoaecdstuacpntoeisstou-nlhsfaiotnncahttisemueravttiovcaholan.tccehn,trhaatticohnisng<su0c.c3e0ssm,gs/urLvifvoarl 4o2r `Submitter: 3M Company, EnvironmentalLaboratoryP.0, Box 33331 St. Paul, MN $5133, USA. DATAA QUALe ITY em ee erson Reliability: Kiimisch ranking= 1 BReEPFeERROENCBES OO OOOO "Thisstudywas conduatcWitldeifde Intemational Li., Easton, MDat the request of the 3M Company. ooTHR ER Last changed: 5/300 w ENVAMRD(2002)17FINAL (RDoabpuhsntiSatmuadgynaR)eport Reference No. 9 - Semi-Stat Life-Cycle Toxicity Test with the Cladoceran TEST SUBSTANCE OIcdetnatnietsyu:lfoPnoitcasasciidu,m1,p1e.r2f2l.u3o.r3o4o.c4t.a5n,e5s,u6l.f6o.n7a7t,e8;,8m-ahyeptaaldseocabfeluorreof-e,rrpedotatsosiausm saPlFt,OCSASor#2F7C9955..39.3)(1. sRteomraerdkusn:deTrhaembtieesnstucbosntdaintcieonissapwhirtoeiepsotwidonegr.. PuSraimtpyldeetwearsmitnaekdentorboem903.M49l%otbnyuLmCbe/rMS2,17',HSLaHmMpEl,e w"aF-s NMR and elemental analyses techniques. METHOD TMyepteh:od;SemOiP-SPtTaStc5L0i.f1e3-0Cy0c,lOe EToCxDiciGtuyideline211, and ASTM StandarEd 1193-87. G`YLePa:r coVmepsleted: Study completed 1999. Report completed 2000 SSpuepcpileise:r:DaIpnh-nhoiuasemcauglntuares, Wildlife Intemational, Ltd, Easton, MD, USA EAnxaploystuircaelpmeroinoidt:or2i1ngd:ayPsFOS measured on days 0.2, 11, 14, 18, and 21. aStnadtitshteicallenmgeithhoadnsd:drSyurwveiivaglhtdaotfa twhaessuervvailvuiantgedfiornstf-sgetn-egreanteironatdiaopnhndiadpsh.ridS,urvthievanludmabtearwoefreliavenayloyuznegd `unsoimnmguiFtiysheurs'isngexSahcatpitresot-.WilRke'psrotdesutctainodnfoarndhogmroogwetnhei(tlyengotfhvaanridandcrey uwseiingghtB)artdlaetta'swetreset. evAanlaulaytaedisfoorf vcaormipaanrciesoannd10Duthmeetnetgsattievset cwoanstoulse(dpt<o i0d.e05n)t.ify Atlrleatstmaetnitstigcarloutpesststhawterweerpeersftoatrimsetidcaullsyinsgig4nifpiecrasnotnailn cToemstpuotregrawniitshmSaPgeS:SI<PC24V-ehrosuirosno2l.d0aotrt"etTOinXitSiaTtAioTn Release 3.5" statistical software Pretreatment: None. Test Conditions Dmiilcurtoioornganwiastmesr:an0d4f5ineppuamrtifcilletsered well water passed through a UV sterilizer to. remove. DilutionHwaardtneerscsh:emistr1y24(d(u1r2i0n-g12t8h)em&g-/wLeaesk pCearCiOo,d immediately preceding the test): AplHk:alinity: 816298(10684-31)72) mgas/CaCLO, Toc: Conductivity: 3<219 0(3m1g5-L340) rmhosiem CNaa/Kgratriaoti:o: 2315133/.6562 wSatsocsktaenddtuensttilsoalllutteisotnspurbesptaarnacteiowna:s dPirsismoalrvyedstporcikors(o0luutsieo.n wAafserprmeipxairnegd,itnhediplruitimoanrywastteorckatso4l6utmigo/nL.waIst pnroompionratliocnoanlcleyntdrialtuitoends woifth1.4U, 2V.9,st5e7r,il[iz1e,danddilu2t3iomng/wLa.teArlltotesptrespoalruetiofnisveapapdedairteodncalleasrtaoncdk csoollourtliesosn.s at ERxepnoeswuarleravtees:selEsv:er2y5M0-onnLd.aypl,asWteicdnbeesakdearysacnodntFariidnaiyn.g approximately 200 mL test solution. The dNeptuh wmaosfabrpepprleoixciatmreast:ely10cm. us ENVIMRD(002)I FINAL NNuummbbeerrooffctoenscteontrratgioans:pne6riprlesupslimcnasetgea:ti|ve control Fceheodwinagt: 15E0a0chmgtesTtScShIaLm)beanrdw0a.s60femd L0.o3fmSeLleonfaYstCrTum(acampirxitcuorernouftuymeas(3t.,5Cero10p"hycleIll,siamnL)d tornocuet dLaiiglyh.ting: Colortone 50 fluorescent lights. Intensity ranged from 329 - 383 lux at the water sWuarftaecer.chPemhisotrtyoofponefew1r6a-inhodourodlsdlsigohltu,t8i-onhsoudrusrdianrgktwhietsthud3y:0-minute transition period Dissolve8d3o-xy8g9enmyra/nLg(en(eg0a-t2iv1edcaoynst)r:ol exposure) 8834--9809mmeyl/LL*((1482mmyexepxopsousurree)) PH rang8e1(-08241 (dnayesg)a:tive control exposure) 88.4 2-8855((1482mmeg/Lexepxopsousruer)e) Test tem1p9e.r4a-t2u0r.er1aCng(en(eg0at-i2v1e cdoanytsr)o:l exposure) 1199..44--2109..15CC*(1(248mgm/eL/Le.xpexopsousruer)e) * (Measurements discontinueda Day 3due to 100% mortality Element basis: Survival, reproduction and growth. Effect concentrations based on survival. Method of calculating mean measured concentrations: aritimetic mean RESULTS NMoemaisnuarledcocnocnecnetnrtartaitoionnss::Ne<gaLtOiQv,e c1o5n,t2r9,,15..46,,2192,,52.74,,4181,m2g3/,L46 mg/L. Element value: 2211-ddaayy NLOOEECC*= 2142mmgg//LL 2210-ddgaeyneMraAtiTonC=acu1t7emsgu/rvLival NOEC = 12 mg/L. All cement values based on mean measured concentrations. Ahingahlytpiecarlformmeatnhcoedolloigquyi:d Ancahlryosmeastoogfratepshtysolwuittihonsmawserse sppeercftorrommeedtriatc WdielteicftieonI'nt(emHaPtLiCo/naMlS)L.td. Wushiengn. rdeestpeornmsienifnogrtthheecotnesctensturbasttiaonncoeftwhaes tuessetd.subsNtoancaettienmptthewtaesst smoalduteiotnos,qutahntsiafymeonantdhemobsatsipsroomfiinnednitvipdeuaakl ipsroomceerdiucralcormepcoonveenrtys.of TmahterixLOfQorti(fmiciattioonsf aqnuaalnytzieadtiocno)ncuwrarsen0tl4y58durmign/gLsaimnpltheisansatluydys.is wTash.e 1m0e4%a.n sMoelaustuiornesdravanlgueedsforfomn9e0w tsoa1mp0l8e%s orfannogmeidnaflrovmalu9e4s.toPF1O2S1%waosf sntoambilneatl.hrouMgehaosuutrthed rvealnuweasl from the periods. old no ENVIMRDQO0)I FINAL Summaryof analytical chemistry data: |CNoonmcienntarlatTieostn my | Measured duplicate rveaslpueecstiavtel0y,,2,m1g1, 14, 18, and 21 Days| MCeoannceMnetraastuiroend|| A Perocfent NagatnUeAConralA[L78110720, 158,156, 138, 147,136,132, 138, 148,150] " 15 : hiLiS s ISI TR TR |W] ST 35977,587,565. 573, 563. 559,536, 59,535, 575,538 wr = [13ren EREW] - 3 paphids42,dea2da3ft0r .[3-2day4s3e05xp,o,s3u30r6e,0B561,,2438.2580.a 3 7.3,480,49.1, 49:4 all daphnids dead afer 2-day [= =] oNlOdTsEo:lutViioenasndumreiansgureaecdhcwoneceekntorfatthieontsestw.erDeaydset0e,r1m,inaenddf1o8marne "wne(wre"naenwadldsaoylsa2i,on2s4), aanndd2S1oraeresp"oonldd"ing BiologicaSluOrbvsievravlatiAolnlssurviving ist generation daphnids appeared normal at et termination. Survival in gthreou2p4. and 48 mL treatments was satisticaly significantly diferent from the negative control oRnepDroadyuc9t.ioTnh:eDBaopnhfeirdrsoniintthesctonsthroolweand tthraeartempernotdgurcotuiopnsw<a1s2mngo/L.ssigtnairftiecdanptrloydruecdiuncgendeoinnaatneys rtheeasmteatnitstigcralouanpal<y1s2ismgof/tLh.e(pre2p0r.o0d9uc)tiTohneda2t4a adnude 4to8amseatlisttrcaatlmyesnitgngirfoicuapnst ewfefeectnootn siunrcvliuvadle.d in G<r1o2wmtgh/:L,TwheereBonnoftesrirgonniifiecasnttlysrheodwueceddtihnatcommepaanrilseonngttho tahnedndeegyatwieviegchotnitrolth(e>tr0e.a0t5)m,ent groups Sweacsa9n5d%G.enSeurravtiviaoln iAnctuhtee 1E.5x,po2.s9u,r5e.:6A,1f2,ra4ndho2u4rmsgo/fLexupcoastumreen,t sgurrovuivpasl wiansth1e00n,eg1a0t0i,ve1c0o0n,tr0o,l anengda0ti%verceosnpterctoilve(ly=. 0S.u0rv9ival i the 24 mi/L treatment group was significantly differen from the `Summary ofPercent Mortality Mean Measured Concentration, mg Negativ1eSContd 29 56 12 -- L rs4 [Day?[Dayid| Dayal Foe NE NY TT 10 TvTW SN SNE 10 I | 100 [toeToe iow 120 ENVIMRDQ00)I FINAL Second Generation Mortality Vian MessedConcentrations, gL | NTumobler|aNfaerrnb4e%r-hAovuers] CamfaivePercentDead [CCFF NemmeCom--a s0r [ =B p35| 5i6? [a e oSrmS ST m Ty oy ------;: RNAIBUN 00K OR. 0%MONI) NPI SummaryofLength and Dry WeightofSurviving IndividuallyExposedFirstGeneration Daphids | Vea MicauredConcentration mL| NSDuuarpmviibnviiednosrgf|| MTooaunlSLDe,nmg,n|| MDrye+WSeaiDghnmtg NegatvsisConial [0 ae0ls| oum-oos [ 56 o2 Ti4ee ivn ons|| momioromnees | T - 30 T T asem e |_omroom | 3 er Reproduction [ican Measured| Naberof MeanLive Young]|FitDayof| Tout Norn of Toa Number o [age at`emr yCommo_|[SDuorp1vhinvii0dns|g|| SuDrav1pivn2iiend1g(A9-d2SuDlt | Repr9oducT tion| Do Ne e/oinmaa mtoebsd iT le| Abort" o0efd Ege CySs T Ts oTThoewiesrT TTo o 0T T 00]| [se ToTwe mpe sTo 10] s I~ t Ta e v coxcLusions sSTheenurTeebawnrslaeyttnrcoeondaacudecvnneetdrrscaetvieeofanfelc<ts12anmsuervivflaol,.2e1pdaryso. duDaocphtngiiraoowtmnhagonfaDeaxpphoisead1m0ag.2n4a eaxnpdos4e8dm1Lh.e htaeds A5u1t3h5o.rUSaAn.dlor submitter: 3M Company, Environmenisl Labortory P.O, Box 33331 St. Paul, MN DATA QUALITY Reliability: Kiimisch ranking I. ENVAMRD(2002)17FINAL a ee ------ "Thisstudy was conducted at Wildife International, Ld. Easton, MD atthe requestof the 3M Compan. OTHER Last changed: 5/300 " ENVIMRD(002)I FINAL MRoybsuisdt(SMptsuiddyopRseipsobrathiRae)ference No. 10 - Flow.through Life-Cycle Toxicity Test with the Saltwater TEST SUBSTANCE OIdcetnatniteys:ufoPnoitcasasciidu,m1,p1e.rf2l,u2o.r3o3o,c4t,a5n.c5s.ul6f6o.n1a7t.e8;,8m8a-hyeptaaldseocabfeluorreof-e,rrpeodtatsosiausm sPalFt,OCSASor# 2F7C9955-.39-3)(1sRteomraerdkusn:deTrhaembtiesctntsucbosntdaintcieonssawprhiiortetoptoewstdienrg.. PuSraimtpyldeweasttaekertnomfbrieo9mn03.e4M9d%lotbnyumLbCe/rM2S1,7.THSoaHmMpRl,e w"aFsNMR and elemental analyses techniques. METHOD. otters "MTeytpeh:odF:loOwP-tPhTrSou8g5hc0h.r13o5n0ic `GLePa:r cYoemspleted: Study completed 1999. Report completed 2000 SSpuepcpileise:r: MyIsni-dhoopussiescublathuireas, Wildlife Intemational, Lid. Easton, MD, USA E`Axnpaolystuircaelpmeorniiodt:or3i5ngd:ayPsFOS mezsured on days 0.7, 14, 21, 28, and 35 cSotanttiisntgiceanlcymteatbhloedss:to iSduernvtiivfyaltrdeaattamenwtasgreovuaplsuathtaetds(hporwioerd tao.stpaatiirsitincgalalyndsiganfitfeircapnatirdiinfgf)ereunscieng(p2<0.x052) fforromnotrmhalnietgyatuisviengcoSnhtaroplirgor-ouWpi.k'sAltlestcoanntdinfuoorush-ovmaorgieabnleeitdyaoaf (vraerpiraondcuectuisoinnganBdagrrotw'th)testw.ereAneavlaylsuiastoedf svtaartiisatniccealantedstDsunwneertet'psertefsotrmweedreuussiendg ato peevraslounaatle dciofmfpeurtenecreswibtehtwSePeSnStIrPeCatmVeenrtsiaonnd 2co0ntroorl"mTeaOnXs.STAAlTl TReeslteamsyes3i.d5s" astgaet:is<tic2a4l-shoofutrwsaroel.d attest initiation Pretreatment: None "DTielsuttCioonndwiattieornsc:heNamtiusrtarlyse(adwuartienrgdtihleut4e-dwteoe2k0p%esriwoidthimwmeeldiwaatteerl,y0p.r4e5cuedmifniglttehreedest): TSaOlCin:ity<: 12002m02g0)%o `Satpopcrkoxiamnatdelyte2st4 hsooulrustpiroinorp0repuasrea.tioAnf:ter Pmriixmianrgy, tshteopcrkimparreypasrteodck sstolu0t0io8n95wasmpgro'poratniodnalmliysedidlutfeodr 0w.i0th05d5i9l,utainond w0a.t0e0r28t0o pmrge/pLa.reTfhivee saidxdisttioocnkasl wsetorcekisnojleucttieodnsinattoctohnecednitlruatetriomnisxoifng0.c0h4a4m7b,e0r.s02(2a4t,a0r.a0t1e12o,f d4e.s6i0remdUtmeistnucotnec)enwtrhaetrieonst.hey were mixed with dilution water (at a ate of 150 mL/min) to achieve the SFtlaobiwlitthyroofutghherattees:t cAhpepmriocxaimlastoellutyioenlse:veEnxvtorleumemleyasdtdaibtlieons oftest water every 24 hours tEaxpcohseudr1e0vtewsoshelosl:esPorinoroptpoospiatierisnigd,es.mysAiftdesrprleaaccehdinginsegxluaasls mbaetuarkietrys, pwiatihs pnlyalcoend imnesghlasseprecterni pdaiisrhiensgwietxhpossiudrees ovfesnsyellosnwmeersehpslcarceeedn iantaScLhegdlawsisthaqsuialriicaonfeialdehdeswiivteh. aBpoptrhoxpirmea-tpealryin5g aLndofptoesst solution. The depth was approximately 6.2 em prior to pairing and 5.5 cm aftr paring. m ENVIMRDQUO)I FINAL `"Twhheitceswtacshdaimpbpeerdsuftorofhetseetcocnhdamgbeenrerfatoimontheexpaopspurreopwrerier2sLmebenatkegrrsouwpi.th IL of test solution NNumubermooffbcreopnleciecnattrersa:tifoonusr: six plus a nega contol aNtuembpeairrinogf.fic per replicate: Fife juveniles before pain, pics (10 dul) when posible WFeaetdeirngc:heFmeidsltirvye dbruirniensghtrhiempstnuaduyp.lii 3 o four timesper day. Not fed th ast dy ofthe test. Dissolve6d0oxy6g4enmraeng(ec(o0nt-ol35exdpayoss:ure) 59-63 mL (13 mpelxposure) PH rang5e2(-08-345 (dacyos)n:rl exposure) est tem5p3er8a.tu4rer(13anmgeg(0-ex3p5osduaryes)): 22445422752251C ((1Cc3onmteolleexxppoossuurre)e) Methodofcalculating mean measured concentrations: aici mean RESULTS MNoemaisnuarledcocnocnecnetnrtartaitoionnss::Ne<gaLt0iQv,eco0n.t05r7o,l,0.0102,860,205.,1075,50,.3143,, 20696, m1.4.L2.7 mg/L Element value 3250--ddaayy (suprovsitvpaalir(ipnrge)-psauirivnigva)lNNOEOCE==C0.50.55mmgigL. 3355--ddaayy rgerporwotdhucNtiOoEnCNO=E0C25=m0/.2l5 mg/L 35-day eprod &growth LOEC = 0.55 mg/L 20d generation cute survival NOEC =0.55mg. (Highest concentration ested) Alelement values based on mean measured concentrations hAingahlytpiecarlformemtahnocdeolloigqyu:id Ancahlryosmaetsoogrfatpehsyt.solwuittihonsmwaesrse pseprefcotrommeedtiatc WdieltcdteionIne(mHatPiLoCnMsS!)L.d. Wuhsienng dspeotnesreminfotirhnepgecrolnucoernotorcautainoensolfftohneatteestwsausbsutsaendc.e ntNhoeaetsetmsptoowans,mtahdeesmtoeqaunadntmiofystopnrotmhinebnatsipsaokf minedainvidpuearlceinstomreerciocvecroympoofnemnattsi.x TfohretifLiOcaQtio(nsimainaolyfzqeudacnoinaciuorne)tlwyasdu0r.i0n4g58sammgp/lL.e iannatlhyississtwudays. 92T.h5.e Smeaamspulreesdcvoalllueectsedfraotm p6r7e.t1esttor1an0g3e%d offonmomi$n7a.l4. 10Me9a9s.u3r%edofvalnuoemsinaflo.r samSpalmepsletsakaetn easttesmtittaetrmiionnathiaodn ranged from $0.8 090o.fno0min%al, Negative ComrolAI<LOQ | % = 034 0-289,0.286,0.231, 0.197,0.276,0.253, 0.227,0.212, | 7a 0.562,0.659,0.581,0.450,0.543,0.542,0.516,0.528, 80 rae [ Mean Measured |JuvenilePre-Pairing| AdultPost-Pairing | l Days 80a.12 I 05 13 26 --T vo Eg 3 Te TT ft eee] 0 = ENVIMRDQOG)I FINAL Second Generation Survival CoincoamntViiosnssurmegd | Tol Number Exposed| NumAholubrsafeer9r:| Perce Survival [Negativ0e05C7onol[| 91 [e Ts o | T TS r T Te e rEI~ rT Adult Mysid Growth CoNinccaeVnirsuwuiroemdmg ||MyNsSiuudmebiNenurmgobfe|r[TomL5enDgtmh,mMea] Dry WeMeiang155,hmg [o|o ame [[EeTnrIoms DTH [ 03s o [ew |p eGis0er0osds| ostisomal C 055 wie |sT ssrom[__om0e56s2"o0o00m620 J Reproduction [VConmcenetrastioen. iT Reps] ReNpDreoadyubsctrive||NYeourngf|WReespYrNoodeuurcDea [veal>ins Negveconl[|AET 5% | TuT |W ooxr |[05505 || o eT To wT TTToomme | wae 0Tr wu Tosw] we |L on [[ TCAA 0T T [a aT 1s oawms]{oso ! ae oaam r| FoT1n M is TowE | N se Tb X [on || 0 F[ oTsA T Tso[T ooems ]s oosoroog | P CT = 5Teauwn]| L TsTT om] in 1 ENVAMRD(2002)17FINAL |EEE 3 EI= 17 Te mr CONCLUSIONS t"oThperoetawsesrieumnopesraftliusotircoaolcltasniegsniuflifcoannatteaetcctosncoenntsruartviivoanls,<re0p.r2o5ducmtgi/oLn ofrorg3ro5wdtahyos.fmyRsepirdodsuhcrtiimopn,exlpeonsgetdh eaxnpdodsreyd twoeiPgOhtSwdeurreintghea smtoastitc 9se6n-shiotuivreexbpioosluogriecaslhoenwdepdoinnotasdvinerhsiesefsftcutdsy.. Second gencration mysids Submitter: 3M Company, EnvironmentalLaboratoryP.O. Box 33331 St. Paul, MN $5133, USA DATA QUALITY Reliability: Klimisch rankin=g | REFERENCES "This study was conducted at Wildife Intemational Ld, Easton, MID atthe requestofthe 3M Compan. oTHER Last changed: 79/01 wm ENVAMRD(002)1 FINAL Robust Study Report Reference No. 11 - Dietary LCs Study with the Mallard duck TEST SUBSTANCE OIcdtenatnietsyu:lfoPnoitcasacsiidu,m1,p1e.r2f,l2u.o3r3o,oc4i:a4n,e5s,u5l6f6o.n1a,t7e,;88m8-ayhepatlasdoecableluorreof-e,rpeodtatsosiusms saPltR, OCSASor#27F9C59.53.0.3()1sRteomreadrkusn:deTrahmebtiesctnst uconbdistitoinsasapnwrihcoirteetoptoeswtdienrg.. PSuarmitpyledwetaesrmtiankeedn fbroem 93M0lo.t bnyu4mLbC9e/rM2%S17,.H-SaHmMpRl,e wVaFs NMR and elemental analyses techniques. METHOD `MTeytphe:odD:ieOtaPryTaScute5:0.2200, OECD 206, and FIFRA Subdivision E. Section 71.2 GYLeaPr:cVoemspleted: 2000 SSpuepcpileise:r:AnWahsisptllainnygrWhiynngchso,sInc. Hanover, IL, USA a`Abnailyltical monitoring: PFOS measured on Day0 or homogeneity infeedand verification, and Day for Test phases: EAcxcploismuart-eio5-nd9aydsays Statistical methods: CPnostveaxlpuoessurcealocbusleartveadtiboyn -pr3oboirt1a7ndaalyyss.is using the computer software of C.E aSntaeplhyasne.sweBroedayppwleiiegdht(0dfaeteadwceornesucmopmtpiaorneddatbay Dunner test using TOXSTAT software. No satsical Test bird age:10days Pretreatment: None "Test conHdiotuisoinns:g and environmental conditions: Indoors In bateries of thermostatically controlled baprporoodxiinmgapteenlsy. 25F.l5oocrans.pacEextoefrneaalchwaplelsn,mceeaisliunrgesdaanpdprfolxoiomratweelrye6c2onxst9r0ucctme.d oCfeiglailnvganhieizgehdtswteaesl Iwdiernetiafnidcasthieoent:ingE.ach group ofbirds Identifiedbypennumber and test concentration. Individuals identified by wing bands. NNuummbbeerrooffdreupclkiscapteesr:rSeipxlfiocartceo:ntfriovles, two for each treatment group NFeuemdbeanrodfwcaotnecre:ntGraatmieonbsi:rdEirgathitonplufsoramunleagtaetidveascobnetlroowl, water from the town of Easton public water supply. Both provaidldibeitudm during acclimation and testing, is ENVIMRDQOO2) FINAL Game Bird Ration S[oyoeBeCaonmMebaal 45%GFRron [ P re m e ANNIE PMo| T hoetneibniBtales gg p] --------1 DrriiaetdyWsvhSealp20e6%0P7rcoPtreoi,vnen f fT e fis Ground Lieitane Cstnan CalPhos a e-- amiTnanyd Miners! PrinGs Bel) -- -- --| SGaLlEtoodmioGeedmaeo) [em010 Fermentation by-products (sourceofunidentified growth factors) Vitamin snd Mineral Premix amin VitoarMminiernal RT ein e ey ey EPiaT f mmT oitneBme,hed h om g] ] TT-- Ba Rie amins T ST Viataamimiiinn K Orso: desided Se mgnese lorodne TRIE SW fem TT ee BProonpdhiylnagxicsomNpoanretment mesn temperature: 38 + XC `AAmvbeireagnetrreolaotmivmeehaumnitdeimtpy:er5a3tu3re1s8%25.2 0.7-C LPhiogthhootpienrgioRdu:orSeixsicceetn Hhohus wihgihtchpecrlodsoeyly approximate noon-day sunlight; serge proximately ENVAMRDQII FNAL ecsatmidiweasprneipdaration: Te substance mined disty ino the ation by means ofa Hobart mcr, No 3iD.io1ctnps:pammopfalnihdnegs:IiefHlromaonomdgehinegehitltoywseoisftotnhansdeoshftigtshhieesstmtaicnoncnegennrvaeeilo.nd.itTheSevasamlepulaSetasemdpclbeoysccsolsoloecFstieonrsgvestdixestaom.plmevsrdifirecohmintohdne srexaopmmopsltuershefcpoeroritihoredsw(eeDcaroyncc5oe)lnflterrcoattmeihdones.tcpoTrnewptoarrav(teoinofenesasunimoDpnleey)s0aa.mnpSdlbeeisaclrhtoymmaemtpnheltersermwaoeinruinc(gwocolonsicacompnslremsihsorusan.adntofone ResuLTs NMEloeemamisenunartledvcaoclnoucneec,netnrtaetaitoinosn:Ds:iNae<rgLay0tiQLv.e5co.=CnGt129o8.l5,(,440.41.,5-21,897354.5.)53.6p.6o1,7m47,3:921,,154376,,2a9n,i51816,56apndn117] pom NNoOEmoCra(lbiotdyycwoenicegnhtsgtiino)n3164.66 pppoms Al ment values based on nominal concenitions SpApneearclftyortnoinmceeadtlriaMctetdWehitoleddcoltliiofogeny(:IHtPDLciCmcItaMosSa)nm.spleWLshd.ewenrkdeientegextrrmhaiicngtiehndgpwteihrteohcrommneactenhnactneorla,tliioqunAiondfalhcyrhsoeemsatotfosepuatbphssoyleutwniiottnhsewmeesrnee SaWoalssutim1oa1nds5,etphtooqsuaianmnethiiafsnydsotmnuodsyt.htepbrToahmeiinmoeefnatinnpdpeivesirdcsueaenlstpsrooencmsoevoretrcypcoeofmrmpaTantueinrso.foarTcihiaecnotLricaOotlQne aw(anmshityweeoddf. qcuNoaotniaacsnuotnr) do1uf0r2ni.onmgi1sa0al3m.%ploMefeaaonsarulryaseidls wvaaSlsaum9e4p.lf7oe.sSaoammlbpilleeinstecsfotolalbeicletirteydicaoamrtpdlocentseimaidnkiaeettiaoantdoDfmaehyaosm$uorgreeadnnegveidtoyosinmfdrio9tm4a9n3g.1e13s01o%9no%f [---------- Homogeneity in Avian diet ConNosmeimnaailoTnesptom Measured Values at Day 0, ppm MeCoanncMenecaasiuorne,d| PNerocmeintalof 2) 952.970,9798W.109.105 |98| is T1r8.o3 T2138.5,22213,41,011833,,1137031:,119643,.1191933| |119 196 6| 1207 Verification in avian diet [CoonvciennmainT,estpo| Measred Dupalyicat0.e Cpopnmceniraions at|coMnecaenaMiioenwa.repdm]| PNeormcienntalof <L0Q 2 I - 366 I 73.2 467,346 718,712 402 ~~i FsVe I 146. 293. 176,172 274,307 174. he 1 291 Pwr] ) 386 550.523 7 ri 0 ENVIMRDEO)ITFINAL Ambient tabi in avian det [(CPomnVceeaOrsMtreoea.dn | pr Measured DupBlaicyast.e oComn.centrations at | MCeoannc-Meeansutr,ed| [MeanDPaeyrcoent of| 55s 1533.10157 Ti:7 ii iso 5 or 0.568 -- io 745 776.779 78 wa] I 174) we remy1 291 [we --1 $37 C--O [[19Sr u6rvival-- and clinical 1150, 1122 observations: No morales ose occured i the Tw contol group, and al ids Sh`oewnsn11no7o0frmtpahlpmidneesagpthpmsentbtsin,neegoadnrudriibntgyehevieupromoseuedxgephooosuungethhepDweeeryi.l.TheThafelirredodvweoeSomeipeee3n9da%onpto1s3is4 1 Comoomorddilniaeteiyonoe,ediovietrgtdnsipogfpsevaeosfineOchneyseme)atairCnyonarecnddnuilcoeowdneissfo1k4n0weopsOks.neisd.lsBsitosulaactdeom1no7o1cnpynpoaseovos]f opiedaydriwipenaigygehadngdpabioe:sivWeihsoenpnwocasostmn,optaerddeedrpforhmo,Dncaon3edorcloomyfuvrgubhui,sonthseerroruwegirhenDneoyP.EReRcocrraymeo nrelcatned TiDhaecyresw15ao3sn2abopadoiyssiewcexiapglohlstyrmgsonaneoadhe,i(b7od0ts.0w9seairdctuncgtiaoonnnSispa3e6le.dg6 pgcomoa.mnpnahireni3g.m1hepnegoD.evy1l+1Gor9orunped. aeDaaypr05o1a5ni dDTaahyer13t.orspw,rordtsi.sae nMiolwDeaerye,sdbiofdwlyeenwceergsohnofCmoothnheeec3.so1repvlrefarmeoeultstenodeRw5e.1rkppMoonomGveends cSro5n3ac2nntpaotmolnasTtor2t2cD9aoynpsemT0a5ioDnays9p3aipnoseptossuon wbeoitgynwegiagnht cwoammmrae6, bseormedeuocen: GcFoerenodssuCNmoenpcsorunmoppi tsityohnA:al Wmihedennrcrormodpuiapersde3dd3ur09i3ntgphetmocorstmvaoyh.goraonlu,hoeehsrsdew.aasuaivmairnkagedDraecytiaondn tihnefsdt nS1i1ay6r,ramsiirnnocailutmdieiosnnignwhaeilrneaccSso.lhToeh,edseon1.s3c1ro0of8eTyssmoiennygm.saswhN,ereeercteoodnnrseldeen0bocerelpssordo,smaFomunyndeodpoet.a,waenrde Percent Camative Morality Concentation, Negative Comroll 0101010 "0 [0 |"o [0| i 91 IF 18.3 s I e 10pT epe etr e Ti et e ] 36.6 Le 107 [oToToT oo 0] i B20 ToTwTe oT0 [0]0] OA SNNE O00 OEOC JO DUOC ROE AAC S00 25 ToToToTmo[0Io110| | 20] s86 0[0To[0[710[20 |30 | 30 | mw To 10[0[20 1 0 |8| 90 |9] | [Nominal [Cm T ExposrePeriod |_R __e_oovenPemed | Wa os Boy |e] Body | Boa | hoy | wep | Change| 58 | Change| Change (ChangeDayl [NegativeControl] 135 | [a TT ms 3 1 [oiToTes [oi | 230[18|a7 | 733 | B36 366 Tn| 7Tas | ie0 [oar 10[oa [use|oaar | |oos[asi| sn ss | | [m2TwTwo Tw T7916 Too [ais | m0 | 6 TusTio[89 om[ia| ose | ess | 203 TT Tse TsTose oom |oa 586. FaLele[anLon Loos | sor [en | um [wosTostTa[64| a [Negative Conwol |~~92 1""isT[n iso | [ I 91 183 wr I{w e s -- 366. Iyen 732 146. or T Th s Tey Ts Te 29 Eo Ca I Tw Finding [[293ppmN=2TS86p|p11m7iN ppm= N=03_] ER etree efter ore] GD L Tract, primarily empty r 1 oy m n TT T izzard contents bile stained ANH AONNORV:SHIN NMSITINESRNR] Intestinal contents, black and tar-Tike Te Keelprominewt 7770 770 3] ] ENVAMRD(2002)17FINAL KT ideyspale T 9 1--7 Lossofmusclemass |2 [5 Spleen, smalland pale. leen. pale hin J yyATT J TT ye CONCLUSIONS "cTohnefiddieentacreyiLnteCrvalvaolfue44f8ortMoa9l5la8rpdpmD.uckTheexpsolsoepdeoftotPhFeOcSonwcaesntdreatteiromnirneesdpotnosebecu6r2ve8 wpapsm3w.i6t7haand9t5h%e wcheii-gshqtuagraeinvaatlutehwea73s.22.p13p.mTehset cnooncmeonrttraaltiitoync,otnhceenntoraotbisoenrvweadsef1f4e6ctcpopnmc.entBraatsieodnuwpaonsr36e.d6 upcptimo.ns in body `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133, USA DATAQUALITY Reliability: Klimisch ranking | REFERENCES "This study was conducted at Wildlife Intemational Lt. Easton, MDat the request ofthe 3M Company. oTHER Last changed: 5/1/00 1m ENVAM/RD(002)17FINAL Robust Study Report Reference No. 12 - Dietary Cau Study with the Northern Bobwhite quail TEST SUBSTANCE OIcdtenatnietsyu:lfoPnoitcasacsiidu,m1,p1e2r,f2l.u3o3r.o4o:c4t,a5n.c5s,u6l6f.o1n,a7t,e8;,8m-ahyeptaadlseocafbleuorroe-f,erproedtastsoiu3mssalPt,FCOASS o#r 27F9C59-53.9.3)(1ReSammaprlekswfaiselsdt:orTehdeuntdesetr saumbbsiteanntceconisdi2tiownhsitperipoorw(d0erte.stinSg.ampPulreitwyadsetetarkmeinnefdro0mbe3M90.lo4t9%nubmybeLrC/2M1S7., H-HMR, "F-NMR and elemental analyses techniques. METHOD TMyepteh:odD:ieOtaPryTaScut5e0.2200, OECD 20, and FIFRA Subdivision E. Section 71-2 GYLeaPr:cVoemspleted: 1999 (Test),2000(Repor) SSpuepcpileise:r:CoWliilndulsifierIgnitneirnaantuisonal Lid. Production Flock, Easton, Maryland, USA. rAenvaelrysteidc-aplhamsoeniHtPoLriCnga:ndTemsatsssusbpsetcatnrcoescocpoyn.centPrFaOtiSonmeiansustraenddaorndsDaanyd0sfaomrplheosmowgeerneeitdyetienrmfieneeddabnyd vTeersitfipcahtaisoen,s:andADccalyifmoartsitoa-bnil1i0tdy.ays EPoxspto-esxuproseur-$edoabysservation 3o 17 days SSttaetpihsatni.calBomedtyhowdeisg:htLdCa.ta vwaelrueescocmalpcaurleatdedbybyDunpnroebtitt'santaelsysuissinugsiTngOXtSheTAcoTmpsuofttewrares.oftwNaoresaotfsiCc.aFl a"Tneasltybsiersdweargee:ap1p0ldiaeydsto feed consumption data. Pretreatment: None "Test conHdiotuisoinns:g and environmental conditions: Indoors in batercs of thermostatically controlled abprporoodxiinmgapteenlsy.23Eaccm.h pEexnt'esrnfalloowralslpsa,cecemileiansgusraenddafplpoorrosxiwmearteelcyon7st2rxuct9e0docmf.galCveainliizngedhesitegehltwwiarse aIndedntshiefeitciantgi.on: Each group of birds dentified by pen number and test concentration. Individuals NIduenmtbifeiredobfyrelpelgibcaantdess.: Sixforcoirol, tw for each treatment group NNuummbbeerrooffcboncoenbtrawtpieohnrsr:eiplsitecvaetene:pfilaveneugatsive control wFaeteedrasunpdplyw.ateBro:thGparmoveidbeidrdadraltiibointufmordmuurliantgeadccalsimbaetlioown,awnadteterstfirngo.m the town of Easton public 14 ENVIMRDQO)I FNAL Game Bid Ration Fine Comba hii [ a pe s e| [[FW4 ohSmoeysbBieinndBadMeesalTM45% Protein ] ee5s----]-- AAvlasSpieesc,i206%0%iFtonon 0 0] ] [GroE und Le ge [EstaColfer TTT oew e-- | gp------ [ViSGtaLatmiFoiodnriamznedd(MFeirmnaeteoPreminGesblows | om | Toi1 FemBmypreoducnts otouetsoFiaeorined Growth aio) Vitamin and Minerst Prix Vitamin orMinera [v1 iamind 7min e] T [[Pr VBioeamoihn, Ae r {rr joy Foie Aci ET -- Poridorine TT MVVaiintagaammnieiKnsne(Veradione dimety pyrimidim bse)| ssg | Ze [[ iSoenlena sa r r PBrroopohdyilnagxcis oNonme pameranttemmpeeranturte 333C AAvmebriaegnetrreolaotmivmeehaumnidtiemtpye:r3a1ture1:42%7.35 1 2C- PLihgohttaipnegr:iodf:uoSirxetseceennhligohtisghutwphircdsh.ay closely approximately 135 fo of huminaton approximate noonday sunlight; average of "Test die preparation: Tet substance mixed diety ito th rion by means oaf Hobart mixer, No ss ENVIMRDQOI FNAL caervas used aD15ni.3cit bpsoamtmtpolcmionngocsfenHttrohaemtoiJgoeennneaidntdyixorfitgrohhSeetschttiso1un1bs7s1toafpncmheeicoThniecnedimgcattvvoesnsla.SampTtblhyeocsseocolSllluedcpitlnedssfiaoxkmsoatmpSelerefvsoe,rdom2imdttehhe,e, Searinfpiiceastifonomsaamcphle)sanfdorthtehecsoeniCroolnc(cnnerastaomnplse)VewreirceactoilolnectSeadmpatlepsreopfatrhaetioootnhrDvaeya0tmeSnat bgriouspasmp(lWeos S"rvepcoClalesctaedraet tsheaened of the exposure prod (Day) fom th comol (on sample nd ch resmeot RESULTS NMoemaisnuarledcocnocnecnetnrtartaitoinosn:s:Ne+gati<vLeOcQo,n1t9,51.84.30,3367.46.,5.731.724,,124961,2,9533,,55an6, a1n1d9617ppm.ppm Flement vane: NDNoiOcmEaoCrrya(LlsiCotdyywce2io2g0h(nt1cg6a8ien-)n28t7975)t3..2pi2oppomppnmm. 1 lent values based on ominal encenuations pAnearlfyotrimceadl MstetWhioddoilfoegyI:ntDeimcattisoanmalpleLsi.werweinegxtrhaicgthed pweirtfhormmetahnacneol,liquAidnalCyhsreosmaotfogtreatpshoyluwtiiotnhs wmearses pSaocsltutrimooanmdse,ethi0ec qsdueaatnmtcitafonyndo(mnoHtsPhtLeOpbrMoaSm)ii.noefnWtihnedeinsviddureaetlseprosmnoisnmeienfrgokhpeceorcmfoplnaantreenonsot.citonnTchsoefuoLtnhOetGsetw(amasinusocdf.equNanotiattnhaeetimropnet) raisng11s5amppplemainnaltyhsiissswnads.94.T7.heSammepalnespecrcoenlterecfoovedreytoefnmtaitoin foofhrtoimfiocgaetinoensitaynailnyzdieedt cnogneeudrernotmy nno1o0m2i:n1a0l7%oMfensomsienadl.vaShaemsplfeosr caomblileentsfoavberiifiScaamtpiolnesiadiketehaatd Dmaeaysu$rerdanvgaelduefsrformom10913 to 112129%% ooff Suom fanalymticalachemristryydata Homogeneity in aviandict ConNceontmraatrion, ppm Nessus Visa Dy 0. yn | WCeonnceNnoastoend,|| --NoPmeincanlto| E --inS 39, i221, 1T 118, 10E 1, 11E 65, 1155 |N 196|S iez Verification inavian diet CoNne`cNmeotnmhrinetaiCloonTn.ersotplm|| Measured DupBl<iacyfa,toe CpgopnmcT entrationsat Cor ncentratiponm.]t PNerecmegnatatof S7i2e 6a7.n34e6 -- T0i2 o] 124063 T17865,10772 --s--T--1w9 | 586 550,523. 537 rr. 16 ENVIMRDQO)I FINAL Ambientsabi n avindiet [|ConDceMmeaaa0tsMiuoerynea.dn | earedDupleivcat5e.C0omncerraionast| MCeonacnenMteraastiuorne,d|[VianDPraeye of] TNeogaetivse ConT wol | ou1s 00 T [> e] Tor T0i2s sT4a7s7s9 wT 2 | --1Te S17E.I17S8 317 To --r-- TSoo.0-c161557 meTe] BiologicValloatbasleirevsaiaonnds clinical abservations: One incidental morality occured i the control group sso e"eru boirf3d,s binoktheencloemaln gtrhoump aweme.imofeDrayel5. yIt vnaostesduwbitshcqTuotlysuotnasnzeesdoconnedDawyil.h Tcwgo Smeaedoaggtrehsosuiognh.outOtthherewtis.e, ll contol Birds wie absrved 0 bc normal in appearance and cTBerhioenugpf.diurrMonirentaatmlhieetnpyeoosceelcxauptreoedsdumrtoerorpuaegrlihoeds.DaoyTch8ceurirenewadlleardnosnDeoagyreo3uapimsn 1t4hee6eSp8d6pamnwdioth1r1s7o1amepopamfotvtheermtdeeSaintghtnss oftoch at concenaions <73.2 pm. Tlhienreealwassign1s1o%f mtorotialyity(iwnintghedr1o4o6pyp.pmAlrlcaitmeerntbgirdosup,naindstweostadgdriotiuopnailsbpilrdyseddisnplosyled SaephpneinosrneosccaunrdedbeohnavDioary9fotro heset Geemriaa,of th Tet. Recovery with orl amare and TSihgenrsowfatsox0ic%itymoobrsaelrivteydopcricoruridneoagnthDiayns,c6e.aanlde7)dorspbpiedssanncteh,ed2u9c3epdmacetisone1 mgroulp. (cosnovuunldsioannsd. maRteicoonverIyehwrigtyh, nwoirnmgaloaopppe,arJaonscse onfd.coobredhianvatiioorn,oclcouwreesd1amn. Dwaeyaknes1s0 atnedt emintion "iTrheesrtemewntasgro1u0p0.%Smiganrstlotftyox(ioccictyuorbisnegrvferodmprDiaoy(03dtehartohueghluDdaeyd 7a)ufeo dbissppeianratnhce,58r6edupcpemd orewaectionmbto wsetsimkualeiss(,sloounwde ahndmmoAtigoin),, plretohsatragtye, pdoespurerses,ioann,d wcionmgoldsroonosp,. loss of coordination, TDhaeyre4wafsor1b0i0r%d mIonrttahleity11(7o1ccpuprmingtrfeartommenDtayEr2p(.noteSdigonns Doafye3xifocryDaopyse2reaefdemporoino)0thrdoeungth dienpcrleusdseidon,3wiinegddroaapppeJoasrsanofcceo,ordriendautcieodn,reKaocwtieorn,i wSesmkanelss,(saonudndloawned.imbotgioyn..Iehargy. foedrysweainghtbogdiyn;weWihgehtncaommopnagredhe0tihrkcomivnolcognrcoeump,ratthoerneswe<rTe3.o3 papppmarentDurreiangmeDnayesa0e.3 S20n5a.iacnldly55s6ignpipfimcanttrersemdeucnttiognrsouipns.bodyBoWdeyighwteisghitn foffbsodscwoeuidghntltosbseodceciumrmeidneid tfhoe 11466%, ww EnvIMRDGIOITENAL xpi the 171 pm group tote moc. SSr Foerepd iCno3cn5moumonpttoisn:nNoSB1s4ptpant.NeoRsem am rdefekdse atoesmtweerS eedsce s sosrarnor a aA a ge aksruei oFnesteatrrwdr SSoeoemfo rBo teosmeop n rgem aandd,Te dogbr Cumin Moray [Cootnon, X PNegauveComwoll 0 | 0[0| 0[0[0|0|0| TS A [36B6 20 ToT T70 e TooTT"oT ["0o |o ol [0 oI 00] ] 146. [ToToTwTo 0|o I10]10] a nt J-- orryWetRigh rseam)Te 7 oee Ta Ft Foy Fi eS CTT 00] a | 30a | 100a |100|a 100 |a doo | 100| on Nominal | WEoboxpaeeohs,u||ree PoG[ereaiosnsd]s|| tWosn f[[aManmesce eeeacsn | oTbtoous|[vee oa|>] | | Scrme eere a te [NegativeComrol| 20[10 | +8 | +23 |wz | 4s| | i 366 73.2 20TnTs [20 Te TT [=a150 Ts a 20 |a4|m | [Tae--T20 T=T5124| 21 | 45 | 3 293 S86 20 TaToe Ss ie 20 er | 34 = 55 | 7 TO =3 oo omy a tes ---- I" [ET -- " a ed eS attoBthoei mecooyntoelgreoa eat <A0 TPn Oueseie)) Mean ere fce omumpton CoN ncapo .om] FDerw oes"t|| Fechrean]| FDoaG cus FG ed[dey Do] EChTew e onn T n ] i T a0 hy ereo CE I r i ele -- Gross Pathologic Observation fom Bid ht Did Sed )Teer =| Finding Abiomvitysores 5erL0Noe 5 e wesh1dr ] eo MCaolnet,roF l | e 146m a|nda U29n3dl eteremin, ed 11(p7p1m eEea n n Ce e| Ctes WaE l o Sr| Eeseco TEE FC BO i rEo eertmial J Fr C n| n risaea o S reh o]| enne || Een Lee} Pe Spleen, grey-brown Ee5ri era]ne FE I eC te ere ENVAMRD(2002)17FINAL CONCLUSIONS "2T2h0e dpipetmarwyitLh Ca 9v5al%uecofnorfiNdoerntcheerInntBerovbawlhiofte1e64xptoose2d89topppemr.fluTorhoeocstlaonpcesuolffotnhaetecownacsendtreatteiromninreedsp(o0nbsee cuuprovnetwraesat7m.e0nt05raelnadtetdhemocrhtia-lsiqtuy,arseivgnaslouefwtaoxsic0i.t02y3.anTdheeffneoctmsorutpaolnitbyocdoyncweenitrgahttiognaiwnaast 7t3h.e21p4p6m.ppBmasteesdt concentration, thenoobserved effect concentration was 73.2 ppm AMiuntnheosroraa,nd5l5o13r3,suUbmSiAtter: 3M Corporation, Environmental Laboratory, P.O. Box 33331, St.Paul, DATA QUALITY Reliability: Klimisch ranking = 1 REFERENCES _ ol "This study was conducted at Wildife International Lud, Easton, MID at the requestofthe 3M company. OTHER Last changed: 5/300 uo ENVIMRD(002)17FINAL Robust Report Reference No.13 - Multi-Phase Expo/sReucorverey Algal Assay Test TEST SUBSTANCE OIcdtenatnietsyu:lfbPnoitcaascsiidu,m1,p1e2r.f2l.uo3r.o3o4c4t,a5n,c5su,l6f6o.n7a7t.e8;.8m8-ayhepatlasdoecabfelorreof-e,rpeodtastosiusms saPltR,OCSASo#r27F9C59.53.9.3()1"RTehemafrolklsowfiinelgd:suTmhmeatresyt issubasbbtraenvcieaitsead dwuheittoptohewdfeacrt(t3haMt Lthoits $s8t3u)doyfhausncbheaernacstuepreirzecdedpeudrtbyy. a more recent METHOD AMSeTtMh-oEd-:35Te.s2t3,pr1o9t8o1c;olOEutCilDize2d01w,1a9s79m.odAiSfiTeMd aSfTePr#t6h6o7se described by USEPA - 6009-78-018, 1978; GTyLpPe:: NSeomi<hronic: SYpeeacriecso:mSpelleetneads:tr1u9m51capricornutun RESULTS EC Values, mg/L. 95% confidence interval s given in brackets) 4 7 I Sg 8 eT Cel1l1-D5ry (15W-e6i5)ght 122 81-366) 128 (46-372) 146 (332350) 2(NCoelClo-nC,ounLitmits) 9919-398) 98 (16-431) 95 (12-455) Remarks: The satistical program used was questionable, DATA QUALITY aRcetluiaalbilciotnyc:enKtlriamtiisoncsh rwaenrkeinngot= 2m.eaTshuirsesdt.udRyessautlitssfiwedercreitbearisaefdoonquanloimtiyntalescotnacitentnthregattioinmse.peArdfdoirtmieodn,albluyt, sample purty was notproperly characterized. REFERENCES "This study was conducted by the 3M Company, EnvironmentalLaboratory,1981 OTHER Submitter: 3MCompany, EnvironmentalLaboratory,P.O. Box 33331, St.Paul, Minnesota, $5133, USA Last changed: 5/300 ww ENVAMRDQUFI)NAIL aSRnoubmHumasattcrhSyatobufidlyhtiRysetopofopaErtgthgaRsleoafgenirdceaGnlrceeoxwNatom.hi1naa4nt-dioTSnhuserovfEiFfvafaetlchtosefaoFfdryCMooinfFntainntouhwoeu(asPdiAnMqciupnehaonluoeswsEp(xPropomoempsshua)rlteeeosxp1po4rsCeo7d5t.to02 75.02 for 30 Days TEST SUBSTANCE Oenutintey:loPnotcasssiiadm p1er1fl2uor.oo2ca3ne.su5fon4at4e;5m.3a.y5sb.seo6pbt6eard.ecf1aero.erd7o0p8os14sCu7m8.s0h2,, CPAOSS#o3r79F5C.3995.(3)1Rchesmcaerrkisz:edT.he test substance is 2 white powder, Sample was adolbeled. Sample pury was not The following summary i abbreviated due the fc that his study as been supercedbeyd a mor recent METHOD. T"MuPenrteoh:poodFs:leodwMreetchrooomdumgehwnaadsheoddneibvceolsospaeyd bpryoceGduc&foGr,S5B8ioannodmiiyc SaangdesclooFseFlsyhfwolaleosweFdst,hosUe.SpreEsPeAn,ted107i3n.he GVeiabrcoxmopleted: 1975 Species: Pmephalespromeia ResuLTs 3300..DDaayy LNOOEECC,: 119mmgl 30.Day MATE: 1 mpl and <1.9 mel. DATA QUALITY uRetliathbeiliatnya:lytKiicamlismchertanhkoi=wndag2s.oqTulheissotisogtnudyy satisfied rteforrqiuaay tesinga th time perfonnd, "REFERENCES MThaisssascthuudysewsats tchoenrdeuqcuteesdt oaftthEe G3M&ComGp,anByi.onomics, Aquatic Toicaogy Laboratory in Wareham, omer Submitter: 3 Company, EnizonmertaLaboratoryP.O. Box 3531 1. aul, MN $5133, USA. Last changed: 5300 1 ENVAMRD(2002)17FINAL eRtocb.u(sDtapShtnuiddy rReepproordtucRteifoenr)ence No. 15 - Effect of Potassium Perfluorooctanesulfonate on Survival, TEST SUBSTANCE OIcdtenatnietsyu:lfoPnoitcasacsiidu,m1,p1e.rf2l,u2o.ro3o3c.i4an:c,s5u,lf5o.n6a6te.;17,m8-8ayeptaaldseocableuorroe-f,erpeodtatsosiuams saPltR,OCSASo#r27F9C5-.9350..3()1. Remarks field: The test substance is awhit powderofuncharacterized puriy. e"sTth,e following summary is abbreviated due to the fact that this study has been superceded by a mor recent METHOD L `MweitthhoDdasp:hnAiaSTmMag,na1;95O1,ECPDro,po1s9e8d1,SDtaapndhanridasPpr.ac1t4i-cdeayforReCpornodduuccttiinogn TReesntewal Life-Cycle Toxicity Tests G`TLyPpe:: NAocute static and Chronic renewal Sepaecriecso:mDpalepthendi:a m19a5g4na RESULTS (95% confidence interval is given in brackets) 4i8ehdoauyrEECaC(r(eipmrmoodbuilcitziaotni)on:): 1247.7((2152--218)8)mmgg//LL 2281--ddaayyEECCa(re(rperpordoudcutcitoino)n*):: 11421401-11134)) mmgg//LL 2164.-2d1a24y NOE2C8*-*d:ay MATC: 71m 12mg ""CTuhmiuslatciovnece(nYtroautnigo/nAdauplp)li ( no observed effects on Cumulative results of Young/Adull and Broods/Adultand on the total number ofYoung/Adult DATA QUALITY sbRuaetlmipaalcbetiulpaiultryci:otnyKclweianmtsirsancothtiaordnaesqnwukeairtnee=lgnyo2.tchTmaheriaassctusertreuiddz.yesdRa.etsiuslftisedwaelrlebcaisteedronfornqoumailnitayl tceosntcienngtartattihoenst.imAeddpietrifoonramleldy,, REFERENCES This study was conducbtytehde 3M Company, Environmental Laboratory, 1984. oTHER `Submitter: 3M Company, Environmental Laboratory, P.O, Box 33331, St. Paul, Minnesota, 55133, USA Last changed: 5/300 a ENVAMRD(2002)17FINAL SRaobmupslteSFtCu-d9y4-RXep(oLritsaRletfoefrePnFcOeSN)o. 16- Pimephales promelas 96-hour Toxicity Test Data Summary, TEST SUBSTANCE 9I4de-nXt.ity: _(P1e-rOfcltuaonreosoucltfaonneisculafcoinda,te,1,L1i,t2h.i2u3m,3s,a4l4;,5m.a5y6.a6l7s,o1.b8e5.r8e-fheerpetdadteocaasfiPuForOoS- Lliitshalitu,mFCs-at9,4,CoArSFC-# 20157-7125) saRduejbmussattrankfcsoer:tihTnewesaatcttesuraalm(paclopenpcrewonxatisrmaatttaiekolenoyn f2ft4rh.oe5m%e3stMtesstpusrbuosbdtsuatcnatcneicoeinnaltnohtde#7t1e5.s.t5sT%ahmwepaltetee.rs)t. saNmoplcealicsulaatmiioxntsurweeorefmtahedete(st0 METHOD - MTeytpeh:odS:tatNioctacnuetde. YGeLaPr:coNmopleted: 1994 S`Spuepcpileise:r: PiAmqeupahtiacleBsiporsoymsetelmass Inc, Fort Colin, CO, USA `EAxnpaloystuircaelpmeroinoidt:or9i6n-gh:ouprHs andDOcontent STteasttisftiischalagmee:th7o9dsda:ys.LC. values calculated by Trimmed Spesrman - Karber. LLoeandgitnhg:and0we.gigh6its:h9A/vLerage length = 2.1 +0.3em Average weight =0.069 + 0.03 Pretreatment: None Test ConDdiiltuitoinosn. water: Carbon fered well water DilutionPwHa:t8er4chemistry: Stock anDdO:t8e.st1somlputlion preparation: A primary stock solution was prepared in dilution water 0 yaipeplrdopraiattesetasmaomupnlteofcosntcoecnktrsaotliuotnioonfw4it0h0dimlga/tLi.on waAtlelrtteostmasokleut|ioLn.osfwseorleutmioandpeerbcyondcielnuttriantgiotnh.e SEtxapboilsiutryeofvetshseeltse:st2cLghleamsicsalbesaokleurtsions: Not noted. NNuummbbeerroofffirsehplipceatrerse:ptlwioc.ate: en NWautmebrecrohefmciosntcreyntdruartiinognst:hessixtupdly:us negative control Dissolve6d0.o7x.y2gemng/rLan(gceon(t0rol 9e6xphoosuursr)e:) PH rang4e87(09 m9y6/hLours()5:60 my/L exposure) 58.00-8844 ((c3o6n.t0rmolge/xLpoesxuproes)ure) "Testte1m9.p2:e19r.5arCtanuger(e0- 96hours): 1 ENVIMRDQO)I FNAL RESULTS ENloemmiennatl vcaolnuceen(tSr96a5ht%iooucnrosn:LidNCeeogu=aetsi1ev9ermcaolt(r1l6s33h9.e,2n, 5.6,b1r00a,c1e80,320,560 mg' cRMooernmtcaaerlnkisrt:aytoofncVooanfltutrehoselstr:sepNosoruntbeesdtaancre nfotrhethsetesatmpslameple. No caleuaions were made fo adjust fo the concuusions chTohenecaeendsttrmatisianomnnpoolwfePocfRoO1mSi,9nmign.g 2T4h.i3%alPeucomsuasakbcteadneiuldfeonbdyat, iLnoidte oslexeexhsibittheedeas9l6-ihouersLCosf tohx Submitter: 3M Company, EnvironmenalLabortory P.0. Bo 33331. Paul, MN. 55133, USA DATA QUALITY. li oRfeltihaebislittys:ullimtiasnchcroannkciengr2t.oTnhisedye,swohliutlieonwse:llancdonddeucetienda,tliacokns fanahlyetiscaalmpdlaeaPforr:ydetermination REFERENCES a 3Th2i90s4td was conducted by the 3M Company, Environmental Labostry, Lab Request number MIO1S, omer -- MA - Last changed: 7901 us ENVIMRD(002)17FINAL XRo(bLuisstalSttoufdyPFROepSo)rt Reference No. 17-48-hour Acute Toxicity to Daphnia, Daphnia magna. FC-94- TESTSUBSTANCE 9Id4e-nXti.t_y(:1-POecrtfalnueosrouolcftoanniecsulafcoind,at,1L,it1hi2um.sa2lt3;,ma3y4al4so,b5e85r%e.fhe6erpr.etda6dte7caa.sfl7PuoF,rOo8S- Lliitshaitu,mFCs-al9t4,, CoArSFC-# 20457725) aRnedma7r5k.s5:%Twhateerte)st Nsaompcallecuilasatimoinsxtwuerreoefmtahdeee(s0tadsjuubssttfaoncethinawcattuearl (coanpcpernotxriamtaitoenlyoft2h4e.5t%estesstubssutbasntcaencien the test sample. METHOD. lL "MTeetsthoydp:e:NoSttatnioctaedc.ute eGLaPr: cNoom.pleted: 1994 ASpneacliyetsi:caDlapmhonniitaomrianggn:apH andDOcontent STteasttisdtaicpahlnmiedthsoodusrc:e:EOCb.iavianleudesfcraolmcuUl.aSt.eduEsPiAn-gNETTriAmCm,edDulSupteha,rmMainnn-eKsaortbae,rUmSeAt.hod "Test daphnid age at study initiation: <24-hour "Test ConDdiiltuitoinosn water: Carbon-flered wll water DillionPHw:ate8r.4chemistry: Stock anDdO:tes5t.6somluetlions preparation: Apinary stock solution was prepared in dilution water (0 avpiperdopariatetset saammopulnet coonfcesnttorcaktisoonluotfio1n00w0itmh Ldi.lutiAolnl tweasttersoltuotimonaskewerSeO mmaLdeobfysdoilluuttiionng ptheer NExupmobseurreofversespellisc:a1te0s0: m+i. glass beakers containing 50 mLoftest solution. `NNuumbemrooffbdcaonpechennirtdrsatpieornsr:epfliivceatpel:usSa negative control `Water cDhiesmsiosltvreyddouxryignegntahetsettudtye:rmination: 7780mmag//LL ((contr1olmge/0xpLoesxu0proes)u0re) PH attes8t6t(ecromnitnraotlieoxn:posure) "Test tem8p6era(t10u0r0e rmanLgee(x0po-s4ur5eh)ours) 20.121.0C Element basis: morality and immobilization RESULTS Nominal concentrations: Negative control, 100, 150, 320, 560, 1000 mg/L ue ENVAMRD(2002)17FINAL Element value: (95% co2nfhiodeunrceEiCn.tyer=va3l3i0s g(i2v9e0n3i7n0b)ramckLets) h8hoouurr ENCO,E=C 2=11000(1m90g-/2L30) mg/L `StSapteiasrtimcaanl-KEavarlbueartmioent:hoTdhweitEhCr:mvasleutes0a0nd%95% confidence intervals were calculated using the Trimmed RMoermtaarlkisty: ofVcaolnutersolsr:epNoornteed are for the test sample. No calculations were made to adjust for the concentration ofthe tes substance in the est sample. CONCLUSIONS `"TDhaephtneisat smaamgpnlaeocfo2n1ta0inmign/gL24T.hi5s%vaPelrufeimuuorsotobcetandeisvuildfeodnbayte,& Linitohiurmtdsoaelxteperxerhsisbittehdraesu4l8t-ihnoutrermEsCo.ftfhoer concentration of PFOS, Submitter: 3M Company, Environmental Laboratory P.O. Box 33331 St. Paul, MN 55133, USA. DATA QUALITY oRfeltihaebitleistty:subKsltiamnicsechcornacneknitnrgat2.ionTihnitshsetutedsyt,swohluitlieonwse;llancdonddeutcetremdi,naltaickosnoafnatlyhtiscaalmpdlaetapfuorrt:y.determination REFERENCES 2"T1h0is94s,tudy was conducted by the 3M Company, Environmental Laboratory, Lab Request number MIOLS, oTER Last changed: 79/01 w ENVAMRD(2002)17FINAL RDoEbAusst SoatufdPlyFROetSp)ort Reference No. 20 - 96-hour Acute Toxicity Test on Bluegill Sunfish (FC-99, TEST SUBSTANCE il ISdaemnptiltey:No.Per2.flu(o1ro-oOccttanaenseulsfuolnaftoean,ciiDcd,EA1,sa1l2t 2ma3y,a3ls.o4be4.re5fe.rr5ed,806h,easp6tP.adF1eOc.Saf1hD8uEo.rAo8-salcto,mpFdC.-99w,itohr23.M2 minabis{ethanol] (1:1), CAS # 70225:14-8) R7e5m%arwkatse:r).TesAtllsavmaplleeireapomritxedtruerleatoefttohtehtiesstmisxutbusrtea.nceNoincwalactuelrat(iaopnpsrowxeirmeatmealdye2f5o%adtjeussttsfuobrsttahencaectaunadl concentration ofthe test substance in the test sample. METHOD MSteatnhdoadr:d MEentvhiordosn.mental Protection Agency, Ecological Research Series EPA-60/3-75-009, April, 1975. TGyLpPe:: SYteastic acute Year completed: 1979 SSpuepcpileise:r:LeOpsoamgiesCmaatcfrioschheirresu,s Inc. in Osage Beach, Missouri, USA. EAxnaploystuircealpmeroinoidt:or9i6n-gh:oupris and DO ammonia content TSteasttisftiischalagme:etNhootdsn:otPerd.obit analysis Length and weight: `AverAagevwleeiengghtt==rh02.a680+.g20..611e75mm, PLroeatdrienagt;me0n.t:2 Nfoines/.Lh "Test Cond`iDtiilolnson water: Laboratory well water DilutionDiwsastoelrvecdheomxiysgterny:: 9.3 mg/L AlHkaarldinneistsy:: 235688 mmy/LL a2s5 CCaaCCOO,, Countductivity: 78 50 moe cSotnoccekntraantidonteosft 1s5o0lumtgi/omnL.preTphaeratteisotn:concPernitmraartyionsstowcekrepprreeppaarreedd ibny tdreainosnfiezreidngwaaptperropraitatae awlhiequnottsiomfintgheinsttoocxkicsatntanadlairqduotds.irTeescttotctlohenycteesnttcrhaamtbiweeorrsn.es pTrheepatreestdsboalsuetdioonns twoetarlesnaomtpelde,tonoftoaomn Cpeorncceennttcroantcieonntsradtoisoniongftrhatee:teOstncseu.bstance in the test sample. SEtxapboilsiutryeofvtehsseeltse:st40chletmricgallasssoalquutaironisa:coNnottainnoitnegd30L oftet solution. NNumubermooffbrfeipslehicpaetrresr:eploincate: en Numberofconcentrations: six plus negative control 1s ENVIMRDQU)I FINAL Water cDhiesmsiosltvre5yd0do7uxr8yig4nemgnetrhlaensgt(eucdo0yn:i-r9ol6exhpoousru)r:e) pH ra5ng5e.(08936mhlour:(18melexposure) 523-05533 ((1c8onmoall eexxppoossuer)e) vesetetemperature: Temperature held constant st 22C trough se ofa wate bath for test RESULTS NEloemmiennatl vcaolnuce:nt(r9at5i%oncso:nfNdeegnactsivientcerovoall, g1i5v,e3n7i,7b,ra1c6k0e,t3)20, 650mg/L. hShoouurt CLoC-=4a36700 GQT900545700)) mmlg 9S66-hhoouurrNLOECC=y3=11Q2m-4g3 m(Cg1r. not caleuaed) A clement values based on nominal concentrations SPtartiistiacnaallyesviasluwaatsiounsodfomocrtaalliltye: LCs values andthe corresponding confidence lis. Quality Check for Test Organism Health: i`LTnhCedbinlaudengi9ltlh5at%sutnchfoinsifhsihdweewnrrceeecimhnaigllsoeon(dgcCeo1dn)dwiiwtteihroena.rwietfheirnencteec9om5p%oucnondf,idAenntciemymciitnsA.repTohretobisnetrveeder9a6t-uhroeu,r Cumulative percent mortality: I Nomimal TeNsetg CoCnoneteraoliaon mg] is P 28houy rs [e So|u7r -hrouss|:V6o=urs - 577s o T ToT O | w 1 Cw io ToeT T oT wlT _iwo J| C Twe [we |wo] concuusions Tinhteereesltosfa2m-pl4e39m6-lh.ourTCheyY6fo-hobuluengoilolbsseunrfviesdhewcastdceotnercmeinnreadto0n bweas3118mmaLl.wiTthhese95va%luceosnfmiudsencbee Sdiuvbimdietdtebrys 3MorCdoemrpaneyx.prEensvsirhoenmreensutlatls LabeormastoorfyPh.e0.coBnocexn3tr3a3t3i1on5o.fPPFaulO,SMN 55133, USA. DATA QUALITY oRfehlnebielstty:SuKblsitmanicsechcorannckeinnagt2i.onThisthsuedst,swolhuitciownselalncdonedturcmtienda,tliaocknos fanatlhyetsicaamlpdleaapfroi.detTehremrienawteiroen wo ENVAMRD(2002)1 FINAL also gaps in the measurement of wate qualiy criteria for number of the concentrations at given time intervals. REFERENCES "oTfhtihsest3uMdyCwoamspaconnyd.ucted by Analytical BioChemisry Laboratories, Inc. ofColumbia, Missouri on behalf oTHER ereeres Last changed: 51200 150 ENVIMRD(002)1 7FINAL Robust Study Report Reference No. 23~ Acute toxicity to Aquatic Invertebrates (e.. Daphnia) "TEST SUBSTANCE hmIedipexntetadidteyc:parfPolodutuoacrstos-iFupmMo-tpa3esr8sf2il0uuomrs(oasolectet,aCnRcAesSumlaf#rokn2sa7)t9.e5;-3m9(-aI3y-)Oacltsuonbeseurleffoenrirced atcoida,s P1F,O1S2.o2r3,F3C.-449.55o.r8.a6s.0p.a1rt7o8f.8t5he. mwReaamdtaeerrktos(:aapdpjTruhsotxeitmteaesstttevlsyaalm2upe4ls-e2u8sisi%nFtgMest-th3es8uub2psp0tea,arnlcmieimxiittnucdroienoectfehntythlreeanteeisgotlnyscouofbltshtbeaunttycelesttisnhuedbrisetatanhnydcleewna(te2erg8)l%.y)cCoilalbteuhutelyaelisetotnhssearmwpaelnrede. Pbaeenlrdofwln.uooraodojcutTsahtnemesseeunltfocwnaaalstcuemlsaautdbiseotnasfnocrea.tshseupmreedsenctheatof atlhle dtioextihcyilteyne wglayscoldubuetyltoethetrheor pwraetseerncwehenofnottehde METHOD -- TMeestthoydp:e:OSEtaCtDica2c0u2te GYLePa:r cVoemspleted: 1991 ASpneacliyetsi:caDlampohnniitaormiangg:naDO, pH, Conductivity, and temperature were monitored daly. S1t9a8t3i)stical methods: EC. values calculated, when possible by standard statistical techniques (Stephan, "tnTce.,st Hdaampphtnoind.sNouHr,ceU:SAO.btained from cultures maintained by EnviroSystens Division, Resource Analysts, "Test daphnid age at study initiation: <24-hour Test ConDdiiltuitoinosn water: Well water from wellsat EnviroSystems in Hampton, New Hampshire. DilutionPHw:at7er5chemistry: TCoOnCd:ucti2vi0tym: g1200 hose *Values measureda time oftet hLoiugrhstidnagrk:.CoNool rwahnistiedfolnuopreersicoedntnoltiegdh.ts, intensity 23 WE/sm2. Photoperiod of 16-hours light, 5 S1t0o0c0kmaLn.d tesTthseolpurtiimoanrsyptroecpakrawtaisopnr:opAortpiroinmaalrlyy dstiolcuktesdoluwtiitohndwialustipornewpaatreerdtion dpirleuptaiorne wtahteefrivaet Etexsptocosnucreentrvaetsisoenlss.: 250 mL plastic beakers containing 200 mL of tet solution. The approximate dNeupmthboefrtoefstrespolliuctaitonesw: aFosurcm. N Numbueroomffdcoanbpcheantierdastiproenrs:repFliiceaptel:usFaivneegative control `WaterDcihsesmoilsvterdyodxuyrgiengntrhaensgteud(y0: 45 hours): 8812--8855mmue/lL ((c1o5n0trmolgexepxopsourseu)re) 15 ENVAMRDQO0)I7FINAL RESULTS PH rang7e8(-0 8648(hcoounriso:l exposure) est tem7p8er-a8tu6re(r15a0nmey(0 4ex5pohsouurres): 22008.7-2"20190CC ((c1o5n0trmogl/eLxepxopsourseu)re) Conducti1v2i0t0y-r1a3n0g0e (p0 o4s5 heours()c:ontol exposure) 1200-1300 mhoslem (150 mg/L.exposure) Element basis: mortality ENloemmiennatlvcaolnuceesn:trations:24N-ehgoautrivEe cCon=ra>l1,y5205,m40L, 60(C,..00n,ot1c50almcgw/aL.bl) hour EC= 49 (43-56) mL. PerMuorooctanesulfonate2c4ohnocuerntEraCt.io=n4ad2jumstpeld clement value: All element values based ohnonoumriaElCc.on=cen1t4rmatiLons wsBuierorvleiovgdiienctgaelromroigbnaseendrivsvamitssiuoaanlnsld:ytahNneidnoercetccyuorrfridevenedcepneiorfcaeslnutlblsaeuntdihavflailerfof2c4cctuarnardnedd48iinmhmotuohrbesi.lcioznattoilonoexfpostuhreer. sTubhleetnhaulmbefefrecotsf Cumulative percent mortality: [Nominal Test Concentration Neg. Control 2dhours|d8hous| 0 es i 40 y NEE SANADTTOTOTRs N Tz 6w105w0 [o o0T TTwi25w Control response: Satisfactory cL oxcLusions L ai"nTdthjeeurstvteacsltdosf4u48b3-sh-to5aun6rcemEC4L.So-.hvoaulyruoeEuisCa1s4fsomurmaeDlallpll(4ht9ioxamicmiatgygxno/fa0t.wh2Lae8)s.midxettuerremiisnedduetotboeth4e9Pmegf/luLowriotohctn9es5u%lfcoonnaftied,entchee Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331 St. Paul, Minnesota $5133, USA. DATA QUALITY Reliability: Kiimisch ranking = 3. The study lacks analytical measurement of test substance 15 ENVAMRD(002)1 FINAL cacrooenuclfedonrtaralastmoiioxcntosunrtiernitabhnuedtetteotxsoicstiohtleyutctiaoonnnniscoitatynb.de spaoTsmhipetlievbeaplsuyirciattyrwiaibtsuetnroetdqutsaoulfiPftiyF.cOiepSnatralasymtcehhtaeerradsciteet(rhaiyzrledednn.eesAg,dldyicatolilkoanlbiaunlitltyyy,.edtaahntedar calcium/magnesium ratio) were not included inthe final report. e REFERENCES e "UTShiAs sattutdhye rweaqsuesctonodftuhcteed3MatCEonmvpiarnoyS.ystems Division, Resource Analysts, Incorporated, Hampron, NH, omer eee Lastchanged: 5/3100 ,---- o 153 ENVAMRD(2002)17FINAL Robust Study Report Reference No.26 - Acute toxicity to Fish TEST SUBSTANCE (H1d-eOncittayn:csPuelrffolnuiocroaoccitda,ne1s.ul1f2o.n2s3t,e3,4A4,m5m.o5n.6i,u6m7,s7a,l8t8m8a-yheapltsaodebceafrleufoerrce-d, 56.9) 0amamsoPnFiOuSm NsaHlt4,#CsAaSt o# 2F9C-09831,. Rwsueebrmseatramknascde:eiTtne0siatsdojspuarstomfppoalrneotlwheaasnadcttauwkaaeltnecrofnr(coe2mn5t%3raMtteisopnrosoudfbutschtteainoecnes,tlos2tu0b#1%s.tainsTcoehpreionptatenhstoelse,asmt$p5sla%emipslwea.taerm.ixtNuorecoaflctuhlaetitoensst METHOD TMyepteh:odS:tatNioctacnoutteed, GYLePa:r coNmopleted: 1974 S`Spuepcpileiser:: PiNmoetpnhoatleedspromelas AEnxaploystuicraelpmeroinoidt:or9i6n-gh:ouprHs and DO content "STteasttisftiicsalagmee:tNhoodtsn:otPeldotted LC, LLoeandgitnhg:anNdowteingohtted:. Average length = 2inches, Average weight= 15g Pretreatment: Not noted Test ConDdiiltliioonns water: carbon fered cityof St. Paul, MN water Dilution water chemistry: Not noted. S`Ctooncckenatnrdattieostnssodloustiinogn rparteep:arOantcieo.n: Not noted. EStxapboilsiutryeofvestsheelst:estNocthenmoitceda.l solutions: Not noted. NNumubermooffbrceopnleciecnattrersa:tiOonnes.: five plus a negative control Water cDhiesmsiosltvreyddouxryignegnrtahengsetud(0y: 96 hours) 4502-5509 mmya//LL ((c1o0nt0romlelexLpoesxuproes)ure) PH rang7e.0(.70.-196(cohonutrrso)l:exposure) "Test tem7p0er7a2tur7e0r-a1n1ge(1(000- m9g6/hLouerxs)p:osure) 21-22C (10.72F) RESULTS Nominal concentrations: Negative control, 10,25, 50,75, 100 mg/L 154 ENVAMRD(002)17FINAL MEolretmaelnittyvoafluceo:nt9r6o-lhso:urNLonCes = $5 mg/L (C.1 not determined) `cRaoepnpmdlaiurecsktseo:d tohna9tt5mh%iexmtciuoxrntefuiardenednocfneothtlitmhittestteswsteursbeusbasnntocatencceian.lc2Nu0lo%ataeitdstoefpmorprotptawhnaiossl mamanatdedrei5a5tlo,%dewAattdeedrrim.tiinoeTnahtlehleyv,ailmtupeeasctrtienpgoofrwttaehsde Pperrefsleunocreoooctfantehseulfoorrgaatneicammsoolnvienutm soarlt what portion of the foxicty can be combed 10. the CONCLUSIONS 4 f"Tahtehetaedstmsianmnpolweocfo8nt5aimngi/nLg. 25% Perfluorooctanesulfonate, ammonium salt exhibited a 96-hour LCs for `Submitter: 3M Company, EnvironmentalLaboratory PO. Box 33331 St. Paul, MN 55133, USA e DATA A QUAY LITY ee LL Reliability: Klimischranking 3 REFERENCES J "This study was conducted by the 3M Company, Environmental Laboratory, 729/74 t0 82/74. OTHER Last changed: 51300 155 ENVAMRD(2002)17FINAL Robust Study Report Reference No. 27 - Acute toxicity to Fish TEST SUBSTANCE a (I1de-nOtcittay:nesPuelrffolnuiocroaoccitda,nc1s,u1l,f2on.a2t3e,,3:A4m4,m5o.5n6i,u6.m7,s1a,l8t8m&-ahyepatlsaodbeceafrleufoerror-e,d 0amamsoPnFiOuSmNsaHlt4,+CsAaSl o#r 2F9C0.8913. 569) sRuebmsatraknsc:e iTnesitsospraomppalneolwaasndtawkaetnerfr(o2m5%3Mtesptrsoudbuscttainocne,lo2t 0#1%. isTohpreoptaensotl,sam$p5l%e wisataerm.ixNtuorecaolfcutlhaetitoensst were made 0 adjust for the actual concentrationofthe test substance in he tes sample. METHOD MTeypteh:odS:tatNioctancoutteed. GYLePa:r cNoompleted: 1974 S`pSeucpipelsi:er:PiNmoetpnioatleeds promelas E`Axnpaolystuircaelpmeroinoidt:or9i6n-gh:ouprHs andDOcontent STteasttisftiischalagme:etNhootdsn:otPeld.otted LC.o. LLoeandgitnhg:anNdotweniogthetd: Average length = 2inches, Average weight = 15g Pretreatment: Not noted Test ConDdiiltuitoinosn water: Carbon filtered cityof St. Paul, MN water SDtilouctkioanndwatteetrscohleumtiisotnrpyr:epNaortatnoitoend:. Not note. CStoanbcielnittyroafttihonestdeotsicnhgemriatcea:lOsnocleu.tions: Not noted. ENxupmobseurreofversespellsi:catNeos:t nOontee.d. NWautmebrecrohefmciosntcreyntdruartiinognst:hefsitvuedpyl:us a negative control Dissolve4d5:ox5y7gemne/rLan(gceon(t0rol 9e6xphoosuurrse)): 3No8t5.re0comredeld a(t1h2i5ghmesetlcoenxc.pos(u1r5e0)mg/L) due to 100% mortality. PHrang7e.0(7.00-(9c6onhtorurosl):exposure) N7o.t07r.e0c(o1r25deamdtLhigehxepstocsounrce.)(150mg/L) due to 100% morality. "Test tem2p0e-r2at1urCe r(a6n9g7e0(0F-)96 hours): 156 ENVAMRD(2002)17FINAL A RESULTS eeeUeLL NEloemmiennatl vcaolnucee:nt9r6a-thioounrs:LNeCg=ati1v0e0mcogn/tLel(,C5L0.,n7o5t,d1e0t0e,rm1i2n5e,d1)50mg/L RMoermtaarlkist:yof9c5o%ntrcoolnsf:idNeonncee conducted on the mixture of tlhiemittesstwseurbestnaontcecianlc2ul0a%tedisofporroptahnisol maatnedria5l3.% wAadtdeir.tionTahlely,valtueestriengporwtaesd Peaprprfpellsuicoenrscoeotoctotahfnaetstmuhielxftoounrragetaena,inacdmmnsoootnlvtieuhntmtsesaotlrsuwbhstaatncep.ortNioonatotfemptthewasfoxmiacidtey tocadnetebreminceomthiebiwmepdacttoo.f tthhee CONCLUSIONS fTahtehetaedstmsianmnpolwe ocfon1t0a0inmign/gL25% Perfluorooctanesulfonate, ammonium salt exhibited a 96-hour LCs for `Submitter: 3M Company, EnvironmentalLaboratoryP.0. Box 33331 St. Paul, MN 55133, USA DATAQUALITY Reliability: Klimisch rankin3g e REFERENCES ee e et "This study was conductedbythe 3M Company, Environmental Laboratory, 10/1574 to 10/19/74 Oe el ell Last changed: 5/300 157 ENVAMRD(2002)17FINAL RFaotbhuesatdSmtiundnyoRwe(pPoirmtepRheafleesrepnrcoemeNloa.s) 28 -- Acute toxicity of P3025 Developmental Material to TEST SUBSTANCE ademnmsiotnyi: umPedrefrliuvoartoivoec.tyl(s1u-lfDoencaatm,indiiudme,cyNl-ddiemceylt-hNyNl-admimmoentihuyml-s,alstalmt awyitahls1o,1b2e23re,f3e.r6r4e.d5t.o5.a6s.F6l.u7o.r7o8a8l.k8y-l epadecafluoro-1-octanesulfonic acid (1:1), CAS # 251099-16-5) Rlaebmoarraktosr:y aTsheP3302M5.proTdhuectisoanmplloet nwuamsbelrabewlaesd LFo-t1116. 15T,hLeotte1t. sTamhpeletesits sLa-m1p4l3e94israefmeirxetdurteo boyf hthee tteesstt speurbfsltuaonrcoecheimnicwalast)e.r (Alalppvraolxuiemsatreelpyort3e0d-r4e0a%t tteostthissubmsitxatunrcee., 6T0h-e7t0e%st swaatmeprl,e aapnpdea0rs-5% beofa 2repshidausael dmiasdpeers10ioandj(ulsetafrorltiqhueiadctwuiatlhcoopnacegnuterastoiloind)ofwthhiechtertapsiudblsytasnecpearinatthe atfetstrsaagimtpaltieon. No calculations were METHOD -- TMyepteh:odS:tiOcEaCcuDte203 YGeLaPr:coNmopleted: 1996 ``SSpuepcpileise:r:PiNmoetphnoatleeds. promelas Ex`Apnoalsyutircealpemroinoidt:or9i6n-gh:ouDrOs, pH, temperature, and conductivity were monitored daly. Swtaasticsatliccuallatmeedthuosdisn:g FLishLer'vsalEuxeasctcaelscsulated using the Trimmed Spearman-Karber method. "LTeenstgftihshanagde:weNiogthtg:ivAevn.erage lengt=h 11.3 mm, Average weight = 7.8 mg. LPoraedtirnega:tm0e.n2t6:9N1o1ne The NOEL "Test ConDdiiltuitoinosn water: Dechiornated City of Duluth, MN tap water. Water was crated for 24-hours prior 0 use in the test. DilutionHawradtneerssc:hemist4r8ym:g/L as CaCO, pLiighting: Cool-8w0h8ite fluorescent bulbs. Photoofp16e-hrourisloighdt,S-hours dark used. Stock aNnodtrtaensstitsioonlupteiroinodpnroetpeadr.ation: Water accommodated fractions. Test solutions were ptersetpsaurbesdtainndcieivindu4aLlloyffdoirluetaicohntewsattree.pliTcahteescoolnucteinotnrsatwieornebvyigmoarsousslayddsittiirornedofforvi2g1o-rhoouusrlsy (svhoarkteenx 17210 1/3 solution depth). The aqueous phase was siphoned from the vessel a mid-depth CStoanbcielnittyroafttihonestdeotsicnhgemriactea:lOsnocleu.tions: Not noted. Elixnpeodsiudrsefivtetsesdelwsi:th4-sLtogplpaesrssjtaosaccocnotmaimnoidnagt3e-oLoxyfgteesntfsloulsuhtiionng.ofThheaedjsaprascwe.ere sealed with Teflon Numofbrepleicatres: two 158 ENVIMRDQUR) FINAL WNNauutmmebbreecrrhooeffmcfioisnstchreypnetdrruarrteiipnolngicsta:theer:seteeondpylus anegative contol Dissolve5d.1ox1yg4e6nmraanlge:(c(0om9o6lexhpoousrsre) PH range78:.8070-189$.0628mhgo(/ucLron:t(o7l00exmpgo/sLuerex)posure) CTeosntdutcetmi7pv7eir8tay-tr7uar9ne9grsa((n10g09(06m0og9uerx)hpoousre)20.8 20C Remarks11:255Ox1y31g32e4nmbwbaosoasdxdedc(o00noh0elmehgxepaoedsxsupproaesc)uere1) he ars before sealing nly and sc {aecstheosbscer,vaptaironicpaelriaoldy. inThheed7i0s0omlLvd.opxyogesnecnctoenntcaenromns.wer supamid mi RREeSUsLTSsLl nd ENloemmiennatl vlaolduieng(9c5on%cceonntfriadteinocnes:iNnetgiaatlivse 24hour LL =G13 gc3oe6n5tnr-o6li7,n54b)0r0am,ckg7et.0s,) 100 mL. STh2hohuouurrr LLLLL===y6$690257 (5(55552143--666046430))) mmmpgLl... 96hour NO=E<4L90 ml. A lent values based on nominal concentrations. acBobinsotlerorogvliecddaulirnifonabglstehroevfasthtie.onfsiMsoharaftttaelrtihte9y67w-0ha0osumrbssy:erNveoexpdmoosinruarilehiectorynecmoearnitnaribanntgoiroenmaxlp2ob4e-shhaocvuoirno,rcoaonnbrdsaei2rovnesod.h winSrtehepunreegastseivone 96-hour. No abnormal behwasvoieorr nthe400 mL. expose contemeion Cumulative percent mortatty: -- "NominalLoading 0 I oT oT Test Concentratiomng,/L | 24-hours 072-hour TToow 75010 tEoTo {iw MLoowreasltitcyonocfecnotnrtartoilosn Ncaounseing 100% mortality: 1000 mgr. CRoenmcaernkisa:ioVnaolfuetshe rfesptorstuebdstaarnecefiohtehe04tesstamspalmep.le. No calelatons were made to adjust for the coxcuusions iTnhteervtaesltosfa$m3p3le69064-hmouLr.LLThfeor96t-hhoeuranmoionbnseorwvewdasfedcettesrlmivneed(tNoObEeL)S6w2ame4l9.0wimthia.95% confidence 15 ENVAMRD(2002)17FINAL `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, $5133, USA DATA QUALITY. Rineltihaebitleisttys:olKultiimoinsschanrdanskaimnpgle2. puTrhiteysitsundyolsaucfkfsicaineanltyltyiccahlarmaectaesruirzeedm.enAtddoiftitoenstalsluyb,sdtaatnaceiscofnocaerntmriaxttiuornes aalnodne.toxAilscoi,ty sucpaenrnsoattubreatipoonsiofttivehlyteastttsroilbuutteidonstowidtihdoexcyyglednimceotuhlydlaalmsmoohnaivuemcoPnetrrfilbuurtoedocttoytluhlftooxniacittey. salt REFERENCES "rTehqiusessttuodfythwea3sMcCoonmdupcatneyd. at ASel Corporation, Environmental Testing Division, Duluth, MN, at the oTHER 1 Last changed: 524100 100 ENVAMRD(2002)17FINAL mRoabgunsat Study Report Reference No. 29 -- Acute toxicity of P3025 Developmental Material Daphnia TEST SUBSTANCE aIdmemntaintyi:umPedrefrlivvoartoiovce.tyls(u1l-fDoencaatem,indiiudme,cyNloddiemceytlh-yNlNa-mdmiomneituhmyls-alts;almt awyitahls1o,b1e,2r2ef3e,rr3e4d40.5a.s5F.u6o,ro6a8.l81k.7y8.l eptadecafluoro-1-octanesulfonic acid (1:1), CAS# 251099-16-5] lRaebmoarartkosr:y aTsheP3302M5.proTdhuectsiaonmplloet nwuamsbelarbewlaesd FL-o1t161.15,ThLeottes1.t sTamhpeletesits sLa-m1p4l3e94israefmerirsetdurte obyf tthhee tteesstt pseurbfsltuaonrcoecheimnicawlast)e.r (Aallppvraolxuiemsatreelpyort3e0d-r4e0l%at tteostthissubmsitxatnucree., 6T0h-e7t0e%st swaatmeprl,e aapnpdear0s8o%beofa r2e-spihdausael mdiasdpeer0sioandj(ucslteafrorltihqueiadctwuiatlhcoopncaeqnuterastoiloind)owfthhiechtsrtapsiudblsytasnecpeariatetsheaftetsetr sagaimtpatlieon. No calculutions were METHOD TMeestthotydp:e:OSEtCiDc 2a0cu2te eGLaPr:CNoompleted: 1996 ASpneacliyctsi:caDlampohnniitaormianggn;aDO, pH, temperature and conductivity were monitored daily. cSatlactiusltaitceadlusmientghoStdese:l'sEMLanyv-alOunees Rcaalnckultaestte.d using Trimmed Spearman-Karber method. NOEL value ""TTeesstt ddaapphhnniidd asoguerace:stOubdtyaiinneitdiaftrioomn:c<ul2tu4r-ehsoumrasintained by ASel Corporation, Duluth, MX. "Test ConDdiiltuitoinosn:water: Dechiorinsted CityofDuluth, MN tap water. Water was acrated for 24-hours pDriilourtionuwsaetienrthcehteemsti.stry: piHa:rdness: 4480m4g/L as CaCO, tLriagnhstiitinogn:pCeoroilo-dwnhoitteed.fluorescent bulbs. Photoperiod of 16-hours light, S-hours dark. No. pSrteopcakreadnidnditveisdtuaslollyfutoiroenaschprceopnacreanttiroant:ionWabtyemr-aasccaodmdmitoidoantoefdvifgraocrtoiuonssl.y sTheastkesotleusttisounbsstwaenrcee sinoluItiLonodfepdtihl)u.tioTnhewaatqeru.eoTuhsephsaosluetiwoanssswieprheonveidgofrrooumsltyhestviersrseedl fatormi2d3--dheoputrhs a(fvtoerrtsexti1n2gtfoor113 Eouxrp.osure vessel: 250 mL borosilicate glass beakers containing 200 mL of test solution. The SNoulumtbioenrsowferreeplkiecpattecso:veFroeudrduring the tes. `NNuummbbeerrooffdcaopncheanitdrsatpieornsr:epFliivcepaltuesFivneegative control Water cDhiesmsiosltvreydduoxryignegntrhaensgteud(y0: 48 hours): 8860--9818 mmgL/L ((c5o0ntmroLl eexxppoossuurree)) 16 ENVAMRD(2002)17FINAL PH rang8e0(40-84181hou(rcso)n:trol exposure) "Test tem7p.er9a2t.ur0e0ra(nSg0em(g0/-L4e5xphoosuurrse)) `Conduct2iv0i9t-y2r1a.n0geC(0 45 hours: 142 155 120- 124 mhosiem pmhosiem (contol exposure) (50 mg/L. exposure) Element basis: mortality and immobilization E RESULTS e ENloemmiennatlvlaolaudei:ng(9c5on%cecnotnrfaitdieoncnes:inNteegravatliviegciovnetnoiln, 3b.r1a3c,ke6t.s3)5, 125,25,50mg/L 82hhoouurrEELL ==2711.3 0((198.67:-391.03).mmgg/lLL 48-hour NOEL = 625 mg/L. Al element valuesbasedon nominal concentrations, `SStpaetaisrtmiacnalKaErvableuratmieotnh:odT.he TEhLe NvaOlEucLs awnads9c5al%cucloantfeiddeunscienginStteerevla'lss Mwearney-caOlnceulaRtaednkbytetstheuTsrinigmmtehde TOXSTAT statistical software Version 32, Universityof Wyoming. taBopixpoilecioatgryie.cdalhTeoahblesteheyrfvfaaentcditosnnson:montaDeldapthihnnriotduhsigshiosnuttutdthyhee nwteeegsrtaetwiimvtoehrtcanolonittrmyoo:lr,tnaloaintdiy,mmtiohbemiml3ai.bz1a3ltiiayonndorw6ao.sv2e5rntomtcglei/dnLicaatwlcasanitygmnestnetosstf cafotnecren2t4raatnidon4.8 hTohuer.numberofsurviving organisms were determined visually and recorded intially and Cumulative percent mortality: [Nominal Loading Test Concentration mg' I Negati3ve03Control = 62255 2550 |24-|h__od8-uhourss s| ry0 ie 0 To 5 0 0 50 7s 95. 100 CRoenmtarroklsr:esVpaolnusees:rseaptoirstfeacdtaorrey for the test sample. No calculations were made to adjustfo the concentrationof th test substance inthe test sample. CONCLUSIONS - c"Tohnefitdeesntcesuibnsttearvnacleo4f8-h9.o6u-1r3.2EmLg/L.forThDeap4hn8ia-mnahogonboaseuwravsreddeetffeercmtilneevdel1(0NbOeEL1)1w3asmg6/.2L5 wmigt/Lh.a 95% Submitter: 3M Company, Environmental Laboratory P.O, Box 33331 St. Paul, MN, S133, USA. ENVAMRD(2002)17FINAL t RDeAliTaA bAilQitUyA: LKIliTmYisch ranking 2. The e study lacks analytical measuremee ntof test substance concentrations aainlnodtnheetotxeisctitsyolcuatinonnostabned spaosmiptlievepluyrattyiibsunteodsutfofidciiednetclyylcdhairmaecttehryilzaedm.moAndiduimtioPnearlfllyu,ordoaotcatyilssfulofaronimtiextsuarte T TRhEiFs EsRudEyNCwaEsSconducae ttAeScdi Corporation, Enviroe nmental Testing DL ivision,l DuL luth, MN,Vat theN request ofthe 3M Company. OTHER Last changed: $2400 CuO ENARR 16 ENVIMRDQU)I FINAL Robust Study Report Reference No. 30- Acute toxicityof PFOS to Rainbow rout in salwater TESTSUBSTANCE d1e12n2i3y3:44P.e5r.i5v.or6o6o,c1t.n7c.s5u5fo.ahteep;admeacyalsisoors.be proetfaesrseidum0saast,PCFAOSS#or27F9C59.53.9.3(1.Octnesufonie ci Remarks: The test substan sa whit powderofuncharacterized pu. METHOD ili - MTeytpeh:odA:cuStteansdtaatridcprreoncewdaulreas ffr4T6e-sthionugrAce Lethality of Liquid Efuents (Evironment Canad, 980) GYeIaPrcoNmopleted: 1955 SFpieschiesso:urRcaei:nRbaoiwnTbroowuS,prSianlgms,ogFariadmeersiord FFtihisoshusaaagncedclasitmeaastitolin8nda0ietyissnoaplnti:iwonaottfteonrfoy:etetTdeihnigorna.du selimaed to increasing sls: held st 30 pars per AExipvotsiurceapmeorniiodt:or9i6n-gn;oDwisssolved Staisical methods: Not note. oxEylgeemne,ntpi,vicoensducwtievritycalled for each replicate crc, but not combined forthe whole study. A cumulates mataliy-concemtation lot vas uscd 0 exis th Lo TestcondiDstioildouintusim:o,nanwdacthelro:rideMis0saisbsaiuga30dpeacrhslopraitnahtdotuapsSwaatlnerdyamended with calcium, magnesia, Dilwautetrchie7moi4st-nr5y Gita: CDo0nd:ucti5vi6t-y:9>02m0y,L00 umboscm SEtxopcoksusroleutviaonlpsrepoartatniiotne::s1l0o00tvmgor.lum35e N Numbueraoffmorrepglaibnciatsemsie:n2esesrtes6un 4 ays apa, not replicated LNounmdbiengr:ofc0o7n3ce4nt1rations: plus bak control Water chDemiitsatrhyeddowuvtretneirSatnungdeisegs: 851-2110013 (G0commea) o77l65-r-a38n30ges(Gcoonmte)l) TEPelhsoettmoetpneetmrpibeoardsaisst:u1rm3eo-r(htao0lui-rty4ighho,ur1s3)h:o1w5sCdark o ENVAMRD(2002)17FINAL RESULTS E`Nloemmiennatl vcaolnuceesnt(r9a5ti%oncso:nf5i,d1e0n,c2e0,in3t0ermvagl/)Lcalculated per replicate: 969-6h-hoouurrLLCC. ==1133..771(10077--1177.78)mmgg//LL Mortality of controls:17% (1/6 in both stdics) DATA QUALITY. bRuetliabaicltiutayl: Kcloincmeinstcrhatriaonnskiw=enr2ge. Tnhotis msetausduyresadt.isfiedRaelsculrtsiwtefroeerqrubaailsietady otnesntoamititnnhaegl tciomnecepnetrrfaotrimoends,. Additionally, sample purity was not adequately characterized. REFERENCES Lhiid,sCsatlugdayryw,aAslbceorntdau,cCtaendabdya Beak Consultants Limited, Mississauga, Ontario, Canada for Panaretic Oils oTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 35133 Lastchanged: 7/19/01 16s ENVIMRDQOFI)NAIL Rabust Study Report Reference No. 31 Acute oscty of PFOS to Raabow trout in freshwater TEST SUBSTANCE H1e1t2t2y33:4P4e.f5l.u5or6o6o.c1in.s7s.l8foenpscasdemcayafsusoosb.e rpeoftearsesdiumtosah,PCFAOSS2o1x9F5C3-995..3)(1-Osaneulfonc ci, Remarks: Th test sbsance isa whit powderofuncharacterized purty METHOD TMyepteh:odA:ctSt,anrdaerndwaprloacfeedrur4e8s-fhoruTressting Acute Lethality of Liquid Ents (Evironment Canad, 1950) GVeIaPrcoNmopleted: 1955 SFpieschiseo:urRcaei:nRbaoiwnbTorwoutSprings, Thamesford FEixsphosaagresaperfietodi:nSaothioounr:sno noted ScAnotamslbtyiitnciecadallfommroentthiehtoowrdhi:onlge:Nodirys.noiveeAddcuomxEuylgleaent,emspmHa,vtacaollnuiduc-wtcieovrnictyecmraautisoendloforwassacuhserdepliecastteimastreet,h Lbuotrot Test condiDtiilountsi:on water: Mississauga dchlrinaed tp water Dilutionwater chem3issittarly): Stock solu0ti:on prepara9ti0on:1100400myLlL noted cloudy ENxupmobseurreofverespsleilc:atNeost: n2ocdstsoluuntioonnevowleumsea3p3a|, nt replicated LNondiung:of0mo7r3g4abn1ismenessrel WNautmebrercohefmicsotnrcyendtruartiionnst:h sptulduiess:blak coil,4 psa bark control Di2s7so1l0ve8d ocxomen ranges 5oH0-ra9n5geGsomgl) 774.7553 -8(7coCnovmoe)) C2ontu37c8tDivu0imyboo-mgsen: TEelsetmetnetmppearcastturmeor(a0li-t4y8 hours 15C RESULTS SoEhloeumerntLvCaulue=s7(595(%62con9f5i)demncoelinterval) calculated per replicate: 1 ENVAMRD(002)1 FINAL 9M6orhtoaulritLyCo:f c=o9n.tr9o(l7s.:5N=on13e.4) mglL DATA QUALITY pReelrifaobrilmietdy,: butKlaictmuiaslchconrcaennktirnagti=ons2.werTehinsotstmueadsyurseadti.sfiReedsuallltscrwietrereiabafsoerdqounalintoymitneaslticnogncaetnttrhaetiotnism.e `Additionally, sample purity was not adequately characterized REFERENCES TLuhdi,sCsatlugdayryw,aAlsbceorntdau,cCtaendabdyaBeak Consulants Limited, Misissauga, Ontario, Canada for Panaretc Oils OTHER `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 719/01 16 ENVAM/RD(2002)1 FINAL Robust Study Report Reference No. 32 - Acute toxicity of PFOS toArtemia sp. TEST SUBSTANCE d1e1n2s2i3t3y4:.4P.e5rf.l5u,o6r6o,oc1t.a7n.c8su,l8foSn-ahteep;tamdaeycalallsuoobr-e,rpeoftearsesdiutmoasaslt,PCFAOSS#or27F9C5-.9359,-3)(1-Oetancsulfonic aid, Remarks: The test substance is awhite poofwunchdaraceterirzed purty. METHOD. T`yMpeet:hodA:cuDtreafsttaItinctemational Standards Organization (Vanhaecke and Persoone, 1981) GYLeaPr:cNoompleted: 1985 SApretcieemsi:aAsoruercnea: 5Sa.lt lake Brine Shrimp Inc EAxrptoesmuiareagpeeraiode:st48in-ihtoiautrion: naupul< 24 hours old. cSAtonaamtlbiysittniieccdaafllomrmoentthihetoowdrhsio:nlge:NosDttiudsyns.ootlevAde.dcuomxEuyllgeaemtnei,vnetpHm,voarcltouaneldsiutcwyt-eicrvoientcyceanltcrualtaotnedploftorwacsacuhserdeptloiecastteimastereictsh,e LbutCnot "Test condiDtiilountsi:on water: 30 parts per thousand NaCl solution Dilutionwater chemistry (initia): pDH0:: >306-m8g2l SEtxopcoksusorleuvtieosnseplr:epNaortantoitoend:;1so0l0u0timogn/vLo;lnuomteed1a0smcLloudy NNumubermooffbroerpgleaincaitsremss:/re3plicate: 10 WNautmebrecrohefmciosntcreyntdruartiinognst:he6sptluduyas: blank control >Di6s0somlgveldLoxygen ranges (test and control): P80H-(8e2st and control) E"Tleestmetnetmpbearsiast:urmoerrtaalnigtey (0 - 48 hours): 21 - 21C RESULTS NEloemmiennatl vcaolnuceesnt(r9a5ti%oncso:nf1i.d2e.n3c,e5i,n1t0e,rv2a0l)mcga/lLculated per replicate: hhoouurr EECaCy ==99..44((7134-1212.12) )mgmgl/LL. SMohrotaultitEyCo.fco8n.t9r(o6l.s7: Non11e.9) mg/L 168 ENV/MRD(2002)17FINAL DATA QUALITY `pReAeldridfaiobtriilmoinetadyl,:lyb,ustKalamicptmluiaeslcphucroinrtcayennkwtiarnsagtnio=otnsa2.dweeqruTeahtinesoltystmcuehadasyruarcsetadetr.iisfzieReddesuallltscrwietrereiabafsoerdqounalniotnysitneaslticnogncaetnttrhaetiotnism.e REFERENCES Li"Tdh,isCsatlugdayryw,Aalsbceorntd,ucCtaendadbay.Beak Consultants Limited, Mississauga, Ontario, Canada for Panartic Oils OTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, $5133 Last changed: 6/12/01 1 ENVAMRD(2002)17FINAL Robust Study Report Reference No3. -Acute toxicity of PFOS to Daphnia magna TEST SUBSTANCE I1d1e.n2t.i2t.y3:3,P4e:rf,l5u,o5r6o6o.c7t7a.n8c.s8ulSf-ohnaetpet;admeacyalalosroo-b,e rpeoftearsrseidutmosaasl,PCFAOSS#o2r7F9C5-.953.9.)(1-Octanesulfonic acid, "RTehmeafroklso:wiTnhgestuesmtmsaurbystiasnacbebisreavwiahtietdedpue toothofwfunacchdathraatcettheirsirszteuddypuhratsy.been supercededby a more recent METHOD TMyepteh:odA:cuItnetesrtnaattiiconal Standards Organization (1952) GeLaPr: cNoom.pleted: 1985 Species: Daphnia magna RESULTS NNuommibnearlofcornecpelnitcraatteiso:n2s: 10,20, 30, 50, 100mg/L. 4E8l-ehmoeunrtEvaClue=s 5(895(4%6c-on7f2)idmenLce interval) calculated per replicate 48-hour EC.y= 67 (48 - 92) mg/L. DATA QUALITY. pReelrifaobrimlietdy,: butKlaictmuiaslchconrcaennktirnagti=ons2.werTehinsotsmteuadsyurseadt.isfiReedsuallltscrwietrereiabafsoerdqounalnitoymitneaslticnogncaetnttrhaetitoinmse `Additionally, samplepurity was not adequately characterized. REFERENCES "LiTdh,isCsatlugdayryw,aAslbceorntdau,cCtaendadbay, Beak Consultants Limited, Mississauga, Ontario, Canada for Panaretc Oils oTHER `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesasa, 55133 Lastchanged: 5126/01 mn ENVAMRD(2002)17FINAL oRroablutsotxiSctituydystRuedpyowritthRetfheerHenocneeyNob.ee34 - Perfluoraoctanesulfonate, Potassium salt (PFOS): An acute TEST SUBSTANCE 3 I1d1e.n2ti.t2y.:33P,er4f8l5v,o5r6oo6c.t7a7n.es8u.l8fSohneaptet;admecaayllaulosroob-e, preofaesrseidumtosaalst,PCFAOSS#or27F9C5-.9359.:3)(1-Octanesulfonic acid, wRaesmasrokresdfAieTld:16T-h2e03CMprpirorodouctteisotinngl.otPunruimtbyedretwearsmi2n1e7d.toTbhee 8te6t.9s%ubbsytaLnCc/eiMsS,aw'hHi-tHeMpRo,wd"eFr.-NMSaRmapnlde elemental analyses techniques. METHOD "MTeetsthotdy:pe:OEAcCuDteGOuriadleline 213, EPPO Guideline 170. YGeLaPr:CoVmepsleted: 2001 SApneacliyetsi:caAlpimsonmietlolrjienrga:LNone nominal concentrations ("TCeSsLt).hoSnaenyd bHeuettsoonu,rYcoer:k,ObUiKa,inNeadtifornoaml cBoelsoUnnyitn.umber 32 belonging to the Central Science Laboratory ""TTeesstthhoonneeyybbeeeeatgyepea:tWsotrukdeyrihniotnieatyiobne:eYs,oufenegoafdualcatrine, nosema and amocba. `Varroacide treatment: None within the 4weeks prior to est nition. Test condiHtuimoindsity: 65% + 5% LTiegmhpteirnagt:urCeo:ndu2c5te+dinCdarkness STteostck saunbdsttaenstces:oluItniiotinaslprsetpoacrkatSioolnu:tion prepared in analytical grade acetone 10 a final cfornocmendtirlauttiioonnsoofft4h7i.s8suolgutPioFnOwSi/tuhL$(0n%omwiinvalsuccornocseen.traRteisounl)t.ingFiancaeltotneestccoonncceennttrraattiioonnwsapsre3p%a.red cRoenfteariennicnge 1togxLicaTnrti:toPnrXim-a1r0y0 stt0ocakfisnoalluctioonncenotfradtiimoentohfo3at.e0wwag/suLp.reSpearceodndianrydesitooncikzseodluwtaitoenrs w1e00r.e FmiandaletbesytdciolnucteinntgrtahteiopnrsipmraerpyarsetdocfkrsoomludtiilountiionnsodefitonhiezseedswolautteironcsonittaihniSn0g%| wg/vLsTurcirtoosne.XaStcaebtiolnietysoolfutthioentbeestfocrheetmhiecatolxiscoiltuytisotnu:dyAwdaissppeerrsfioornmetde.t wTahsecahrormioedgeonuetiotnyoafnt8h6ejmgixPtuFrOeSwuasL. taesmspeesrsaetduraef,tesl2ighhtousres.dimeTnhtewtaesst niotteemd.foFromredthae tcolxeiacritsyoltuetsitonallonsolmuitxiionngs;waefreerr2e-hmoiuxresdaptrirooro(m0 pusuer.poTsheoefcaodntmriancitstlaatbioorna.tory considered the solutionsofthe test doses to be homogenous for he dEixapmeotseurr,e vessels: Clean, well-ventilated inverted pee dishes, measuring approximately 9 cm in SFoeleudtiinogns: cDounrtianigntnheg ftihrestafpopurrophroiuartse oPfFtOhSe tdeosste,.beeAsftperro4v-ihdoeudrsw,itdhos5e0d%suwcirvosaequreeomuosvesdu,craonsde bees provided with 50% wiv aqueous sucrose solutions, continuously available through the end m ENV/IM/RD(2002)1 7/FINAL. ofthe exposure period. is 24-hour LDyg = 0.72 (0.60 - 0.85)pg/bee: 4782--hhoouurr LLDugu==00g4.406 ((00.3.32 -00..5455))pgg/lbeeee.: : NegativeContol |0[0 [Solvent Control ee ---- 0| 0 | 33 0.205 [oT er T "10 0.450 0T7720 T77s0Te 0.991 217 a [67Tio[eo | 97 47s 30 io |_t00J Sublcthal Effect-s Percent Knockdown(K)orStumbling (5: ENVIMRDQUFOIN)AIL SolveSnteControl TT, y as [0e [3|3Bm E HSEE a 2 s0] ] [ fre m RCoenfterroelncreetsopxoincsanetsraeisstpuocntsoer:ysaisactor--y dimethote 72-hourLD. 0.11 ghee conclusions "bJTihbeeeeep.otTwahissheiud8mo9sp5err%efsicpuooonnrfsoieodwcetnaacsnessnutlteefrpovbaateletwo7fe2-e0hn.o3au3rmeora0an4l8uL.ptDaTkehfoofr72t0.h4he5oahunednyo3.obbbeseeewreaves.ddeeteermiTneende0wibse 00..3410 Submitter: 3M Company, Environmental Laborstry, P.O, Box 33331, St.Paul, Mines, 5133 DATA QUALITY Reliability: Kiisch ranking 1 REFERENCES T2Wh0ii0s1ldsetudtyerwnaasticoonnadlucLtied,d EaansCoenn,tMalDSactietnhceereLqaubeosrtatofohrye, S3aMndCoHmutpoann,yYaorbk,ReUqKu,esutnNdeurmbceornt0a2ct72b3y, omer Last changed: 5101 mn ENVAMRD(002)1 FINAL cRoonbtuasctt StotxuidcyityResptourdty RwietfhertehnecHeoNnoe.y3b5ee- Perfluorooctancsulfonte, Potassium salt (PFOS): An acute TEST SUBSTANCE i I1d1e2n.t2it.y3:34P4e5r,fl5u,o6r6o.oc7t7a.n8e,s8ulSf-ohneaptte;admeacyafallusooo-be, rpeoftearsrseidutmosaalst,PCFAOSS#or27F9C5-.953.9.)(1-Octanesulfonic acid, ewRlaeesmmeasntrtokarsledafAniaeTlldy:s1e6Ts-h2te0ec3ChMnpirqpiuroerosdtuoctteisotinngf.otPunruimtbyedretwearsmi2n1e7d.toTbhee8t6es.t9%subbystLanCc/eMSis,a'wHh-iHtMeRp,ow"deFr-.NMSaRmpalned METHOD . TMeestthoydp:e:UAScEuPteACOoPntPaTcSt 850.3020 (df), OECD Guideline 214, EPPO Guideline 170 GYeLaPr:CYoemspleted: 2001 SApneacliyetsi:caAlpimsonmietlolrjienrga: None -- nominal concentrations "(TCeSsLt).hoSnaenydbHeuettsoonu,rYcoer:k,ObUiKa,inNeadtifornoamlBceoelonUnyitn.umber 32 belonging to the Central Science Laboratory ""TTeesstthhoonneeyybbeeeeatgyepe:atWsotrukdeyrihniotnieatyiobne:esY,ofueneogfadauclatrine, nosema and mocha, Varroacide treatment: None within the 4 weekspriorto est initiation. Test condiHtuimoindsity: 65% + 5% TLeigmhpteirnagt:urCeo:ndu2c5te+dinCdarkness 9TS0et.so4tckisaugbnsPdtFatnOectSeI:soLSltu(tonicookmnissnoplaruletipcoaonnrcapetrniteorpnaat:rieodn).in aFnianlyatlictaesltgcroandceenatcreattoinoenstopraepianraeld cionncaecnettroanteiofnroomf ddieliuotniioznesdowfattheiscsoonltutaiionni.ng R| e/fLereTnricteontoXx-i1ca0n0t:toSatofciknasoclountcieonntroaftidoinmeothf3o.a0teigwiausL.preFpianarledtesitn `Sctoanbcielnittryaotfiotnhseprteepstarcehdemfircoamldisloultuitoinoson:ftAhidsisspoelrustiioonn.test was carried out on an 86 ug PFOS/uL aascseetsosneedsaofltuetrio2n hboeurfso.r tThheetotvectitiytesmtufdoyrmweadsapecrlfeoarrmesdo.lutiTohneohnommioxgiennge;itayftoerf2thehomuirxstuatrerowoams tuseem.perTahteurceo,ntlriagchttlsaebdoiramteonrtywcaosnsniodteerde.d tFhoerstohleuttiooxncsioyfttehset,teaslt dSoolsuetsio0nsbweerheomroegmeinxoeudspfroirorthe dEpiuxarpmpeootsseueror.efvaedsmsienliss:trCalteiaonn., well-ventilated, inverted pet dishes, measuring spproximately 9 cm in FNeuemdbinegr:o5f0r%epwliicataeqsu:eTohueses.ucrose solution, continuously available NeNguamtbievrecoofntbreoels:peUrnrdeopsliecdate: Ten SRoelfveernetncceonsturboslt:aAncceet:onDei.methote ENVAMRD002)1 FINAL NRefeurenocmefscuobnbscteanntecreatcioronntso:l:ivTeriptlonusaX-n1e0g0ative and asolvent control ddDioosssciaenrgdaedad)mn.idnigEseantctrhlaytbietoienp:wpeaTdshodeuotsboeendetsoonwfteilhrteeerthpaonarapaeexsrtwhaeintdtizahceodu1nuwtiLetdhdrncotaporobtohfane gpdiievorexniddeiesshimtcmaeegmdeica(otdnrecolenynctsrbaewtfoiorornee DorosIeLfarceeqtuoennecy:beOfonrceebeing placed nt the est chamber Element basis: Mortality RESULTS PNoRmOiSnaeleconcentrations: Negative control, acetone contol (10 bec), 1.93, 424, 930, 205, 45, pe Element value and 95% confidence interval: h24o-huourrLLDD.== 31084.9(8/228-1.37.210.)2)gghheeee 997662---hhhooouuurrr NLLDDOxSEy0L===64.107.89(34(.3p.78 -e7-.e6)8)ppefbeee Al clement valuesbased on nominal concentrations ePStrsaottbiiimstattiec|dalpuasEcivknaaglgueS.attuidoeAnnt:'lsPeraiosbsti-ttsqm(uoaprr<te0as.li0rt5ey)gprleoststieodnag(aFiinnsnteythe19l7o1g)ariwtahsm offidttoedstoustihnegse.the TcohnetraNctOlEabLorawteorrey rBieoslpoognisceableotbwseeernva4t.i2o4ns1:ndTh9e.r3e0 bwaesesignificant mortality at al doss above 1.93 ghee witha steep dose Cumulative percent mortality: hee I NegativeComwol |0| [solvent Control 33 0 33 | 33 | 35 | 33 33 | 1.93 0 67 13 9.43204 o 0 e rT 63 ] 90 205 450 [0TwTo "9 T T 00] oT soTes 100 100] `SublethalEffects Percent Knockdown (K) or Stumbling (5): whee Negative Control o Io lo7] [SolvemComrol [0T7701 0[0 0 | 424 [95% 205 450 Tv oT 1 [oT1] "eo T3360] se T3300[0 1 0 | is ENVAMRD(2002)17FINAL Control response: satisfactory Reference toxicant response: satisfactory- dimethoate 96-hour LD. = 0.19 ghee CONCLUSIONS "g"TThhheeedpeoostweasrsieisaupt9mo5nphse%erfwclaousnofrsiotdoeecentpcaenbceistnuwtfeeorevnnaalt4oe.f29463-ah.nodu89r.c53.o0ntuTahgceth9L.D6, f-ornhotheoobhsouenrevryedbeeeffweatsldeevetlerwmaisne1d.9t3o gb/eb4e.e7,5 Submitter: 3M Company, EnvironmentalLaboratory,P.O, Box 33331,St. Paul, Minnesota, $5133 DATA QUALITY. Reliability: Klimisch ranking | REFERENCES more WTihilsdisfteudIyntwemaasticoonanld, uLcdt,edEaatstCoenn,tMraDl SactitehnecereqLuaebsotroatfotrhye, 3SaMndCoHmuptatonny,,YLoarbk,ReUqKu,estunNduermbceonrtrUa2c7t2b3y, 2001 OTHER rere Last changed: 5/101 cre re 6 i determined to be 86.9% by LC/MS, 'H-HMR, "F-NMR and clemental analyses techniques. Cott iE MgCL6H20| 1216 |ml| CaCl, 20,0 4.40 H,B0, mg | [MnC04L 16 4 |H mgr0| To Er rr ||NCau;ECDThAa.2mH;o0T) 0012 | ug | NaNO, | 255TT mg | ENVAMRD(2002)17FINAL "fTielsttratsioolnut(i0o.n2pmre)paprraitoirotnos:use.Individual test solutions were prepared in algal medium at ach of tahpeprsoixximnatoemliyna1l hcoounrcse.ntTrhateiofnisn.a teTthseoluStoilountsioanpspewaerreedclsetairrreadndwictohlormiaegsn.etic stir plates for cEoxnptoisnuirneg 1v0es0smelLso: fStteesrtilseolu2t5io0n.mL glass Erlenmeyer flasks plugged with foam stoppers NAguimtabteiorno:frSehpalikceantecso:ntsiinxuo(uisnlcylautdi1n03g0pforanalysisofexposure concentration). CIeniltliacloualngtaslmceetlhlooda:dihnegm:a1cy0tXome10t"ecreanld mlicroscope. Ncouncmebnterratioofncoenscteendtrations: six pluas negative control plus an abiotic control at the highest `Water7c4he-mi7s6try(:copntHolraenxgpeos(u0re)96 hours) "Test te7m4pe-r7at4ur(e32r9anmgye/(L0exp9o6suhroeu)rs) Light 2le8vel-s:2(30-5C96 hours) P1h9o9t0op-e2r3i1od0:lux24f-ohmoucsooilg-hwthite fluorescent lighting o`bMieatihnoeddoaft -chaolcuurlsa,ti7n2g-hmoeurasnamneda9s6u-rheodurcsoncentrations: arithmetic mean obtained using results RESULTS - NMoemaisnuarledcocnocnecnetnrtartaitoinosn:s:Ne<gLaOtiQv,e 3c7o.n9t,ro6l3,.397,.99,3.5,8.164,38,82.35,5,13392,92m16L, ;331abimogt/icLrpelpulsi3c3a1t=eab3i4ot9icmgr/elp.licate E24l-ehmoeunrtEvCa,lu(ecsl(l9d5e%nsciotnyf=)id1e0n3cemgi/nLt.er(vCa.l1.) not caleulable) 224cchhoouurr EErBCaCr ((garroeawtuhndraetre)c=u94r=(39v3-0e1(44)50)-m1g5/0L).mg/L 4488-h-ohuroEECuBrC(c(ealredaenusnidtey)r c=ur1v1e7) m=g1/0L3(mC.1Lno(tC1c.alncoutlacballec)ulable) 428h-ohouurrEECxC,ao(c(lglrodwtehnrsatie=)t=y4)312(8 m3-g/$L44()C.m1.gn/oLt calculable) 7722--hhoouurr EEBXCC (agrreoawtuhndrear ct=uerv$)e2).=4<9-3811m6y) LmL(C.1. not calculable) 72-hour EC. (cell density=) 120 (92 - 139)mg/L. 7722--hhoouurr EErbCCaye((garroewatuhndaetrec)u=rv1e7)4=(114166 -(42908-)1m4g2/)Lm.g/L 7722--hhoouurr EEBCC ((caerleadenusnidteyr)c=ur2v2e4) (=129034 (27153)-4mg2/2L6.) mL. 672h-hoouurEECiCa(yce(lglrodwetnhsirtay)te==)$2275((21-9621-2353) 0m)LmL. 9966--hhoouurr EErBCCip,((garroeawtuhndaetre)cu=rv1e0)9=(8546-(21625-)1m07)Lmg/L 9966--hhoouurr EEDCC(c(ealrledaeunnsdietry=c)ur13v1e=)(101624-(114024)-m1L3.8) mglL 996-6hou-rEEhCCano((cgeurlowdretnhsiatyt)e==)211736((210369-211891))mmgg//LL i. ENVAMRD(2002)1 FINAL 9966--hhoouurr EErBCCo ((garroeawiuhndraetre)c= u225r=(22v0209e1293)75-) m2/1l8).mglL 7722--hhoouurr NNOOAAEECC ((gcrllowdtehnsriatcy),:a9r3ea.8unmdge/rLcurv): 37.9 mL 9966--hhoouurr NNOOAAEECC((caerleadeunnsdietryc,urgrvoew:th63ra9c)m: L93..8 mglL All clement values based on mean measured concentrations aiStnnahdtiibEsittiCicoanlvvaalmlueueteshsaowndedsr:9e5Cc%eallclcounldfaentisedidetniucesesil,nigmair"teTsawheeurnSdeAercSalStchyueslatgteermdowfbtoyhr lWicinunerdavoeiwnstv"earl,puoeRlsea,ltiegoanrsoewwi6tt.hh12.trraetTaehtsemeanEntCdyryepseErpcCoeannnst ucasunirdnvgeextaphnoedsSughrraeopwicrtonohc-eWrniattlreksa'tsaitotne7s2tdaaatnnadduLs9ei6vneghnoeTu'rOssXtwSesetTr,AerTeesvpVaelecurtasitvieeoldny.f3o.5r.WnhoerCremelallitdhteeyndssainttdaiswh,eoarmreoeganesonnucmnahdlyelryoftdhvieasrgiirbaonuwcetedhs Ri`wneissttuhalntecsqeuoafwlthhveaerresitaandtciaeststai,ctawhlearnetarlneyossttemsennwoterrgmeraoluusypesddiwtsoetrrdieebtucetoermdm,pianterheetdhen0oNn-OtphAaerEcaComnettvrarolilucesu.sKirnugskDauln-nWaeltlti'ste1sett wInastheweodne dHAiengtahel"rymtpiiencriafnlogrMtmehatenhccooednoclleinoqtguriyad:tiAconhnarolofymtsahetesoegorsfatpeshsuybsswtoialtnuthcieomnnsatshwseerseepsetpcetsrorflooumtreimtoernidsc,attdheeWtieslcadtmiieofneanIdn(tmeHrsPnatLtOipMornSoa)mli.Lnted|n.tWuphseieannkg prieensdscipevoinndtsueralefciosovoempreeyrroiffclmucoaortomropicoxtnaefnoncrsstu.ilfficoTanthsietoenLs wOaanQasl(yumzseieddt.coofnNcquuoranrtaeintttealmtyipodtnu)rwwianasgssm4a.am5pd0leemgtao.n/aLqlyuisnaisnthtwiysasstoun1d0y3.t%h.eThSbeaamsmpeelaeonsf Hscooalumlrpeslcetwseedrteaatk9e9ens.ta0tn-Tt21t-0hi9oou%nrosafwndoemrmine9as9l..u0r_-eF1ov1ra0lt%hueoesfafbnriooomtmiinc1arl0e.0p1lTi0cha1ete1,m2e%tahsoefunmroeemadsiuvnraaellduevTaflhoueretmhfeeoarsstauhmreepsdlaevmpaalhleestnafaokre9t6h-e 72-houes was 1039%ofnominal andfo the sample taken at 96-hour, 107%ofnominal. `SumNmoarmyinalofaTneasltyti|caMleachseumrisetdryValduaetsaa:0, and 96- | Mean | Percent of | Concmenetaton, | hours, Respectively, mglL |ConMceeanstruarteido|n, Nomimal | Negative Control A=10g ET -- [se3e79 361596,,368272,,633746 [31699 1T0 w] ] 38.8 7 914832,,917446,,184928 9a3T8 |10 we 6|| 216 331 (3a31biotic 0mm | ns Notanalyzed, 342,356 334299 | 0T9os4 i" ENVAM/RD(002)1 FINAL BioMleoagincaMleaosbuserrevda|tionMseaafnteNru9m6b-ehrou|rs: Percent Percent Persent | Concemntgration, | ofCemlLlsper | InhDibeintsiiotnyvia|| IAnhricbaitUionndevria || IGnrhoibwitthionRavtiea the Curve Negative Control | se0.067| -| - [ - 379 [eosoo0 | 63 130| 13 63.9 585,833 097 f 938 ams 131 aae136 | 143 [sess | er | eer | ne] 235 2338 |wer Tor Toor | STndicate3s29a sigific|ant_isffseroenoce fr|om the oneogartive| control udsionogrthe a| ppropriate e taeste]d est (p <0.05) OCbosnetrrvoaltrieosnpso:nsAef:rsat9i6sfhaocutrosroyfexposure, there were no signsof aggregation or adherence ofhe algae ctehlelfmloasrkpshoinlothgeynwehgeatnivceomcpaorendtotoaotnlhynetgreaattimveenctognrtooulp. In addition there were no noticeable changes in aRlegvaelrsmiebidliiutym oafndGrcoulwttuhredInfhoirbintiinoen:daAylsiaqfutoestohfetehxepo2s3u5reanpdha3s2e9omfet/hLe stetsutdysoclountciolnusdewde.reBdaisleudteodnwitthhe cionnccreeansteraitniognroofwt2h3o5bsmegr/vLe.d bHyowDeavyer9,ofnothaelrgeacocveelrlywpehraesed,ettheecteefdfedcutroinngatlhgeargercoowvtehrywpahsasalegiistatthiec a3t291 mL treatment indicating that PROSwas algciadtthaalt concentration. CONCLUSIONS q"Tuheaepowtaasssdieutmerpmeirfnleudoruosoicntgantehrseuelfcoanlactucla9t6io-nhomuerthEodCs:. and By 95% confidence interval for Anabaena floscell density. it was 131 (106 - 142) me/L, by a9r6e-ahouunrdeNtOhAeEgCrovwatlhuecsurwveereitdweatesrm1i2n4e(d1004b-e 6133.98) mmgg//LL aunsidnbgythgeroarwetahurnadtee1r7t6he(g1r6o9w-th1c8u1r)vem,y/aLn.d 9T3h.e5 mwerLe wniotthedthincealnlydeonfsitthyeatnedstgrsoolwutthiornasteocraltchuelactoinotnromlest.hod.PFNOSo swiagsnsodfetceerlmlinaegdgretogatbieonalogrisatdathiecreantcea concentrationof235 mL and algicidaal 329 mg/L. `Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY. Reliability: Kiimisch rankin=g | ere REFERENCES "LTahibsRsetquudeystwansumcboenrduUc2t7e2d3.at Wildife International Lid, Easton, MD at the request of the 3M Company, oTHER Last changed: 7/19/01 150 'H-HMR, ""F-NMR and elemental analyses techniques. oll Ta [hae|ooes 1 om | H,BO, anz_ 1mer [C T7h 832a 1l mgro| [CottFeColy n61,0 | 2389s6 1 |me yer r| [NaMooi2i0TST gl | [Cc Tou aa17am pg o| |NuE|D6T 00| A2mM er 0| 261 mgt| NaHCO, 300 1 mgr 7] ENVIMRD(2002)1 FINAL "DTihleutpiHoonfwtahteermesdouirucme:waWsiladldijfeuIsntt1ee0rndat7.i5o+na0.l1 Ldu.sinwgel1l0%watHeCrL.purified by reverse osmosis. The tpeusrtifmieeddiweulml wwaatser.preTphaerepdHbyoftahddeinmgedtiheumapwparospraidajtuestevdoltuom7e.s5 o+f0.s1tocuksinngutr1ie0n%t HsoClIutainodnsthteo mSteodcikumanwdastsestterisloilzuedtiboyn fplraetpiaornat(i0.2onj:mA)prpirmairotyorsutsoe.ck solution was prepared in duckweed smteirdipulmateat afcoorncaepnptrroaxtiimoantoefly35214mgh/oLu.rs.The pArfliemrarmyixsitoncgk,sotlhuetiopnriwmaasrystsetodckwitsholautmiaognnewtaisc `pArlolpionrtailotneasltlysodliultuitoends awiptpheadruedckcwleeaerdamndedcoiluormletsos.prepare the ive additional tes concentrations. dEixsppoossuabrleevpeestsieldsi:sh2l5i0d.mi. plastic beakers containing 100 mi tet solution, each covered with a A`gNiutmabteiorno:fNroenpleicates: three plus2 additional replicates for analyticalsamplingon Day3s and 5 Nnuimablelroaodifngc:on5cepnltarnaitsiiroenpsl:icsaiex, 1p5lufsroandnseigraptliivceatceontrol plus abiotic controls at the highest c`oWnacteenrtrcahteimoinstersyt:edpH range (0-96 hours) 7849--8579 ((c2o3n0tmogl/eLxpeoxspuorseu)re) oMbeitahinoeddoaft Dcaalycsul0,a3,tin5g, maenadn7. measured concentrations: arithmetic mean obained using results RESULTS cNoonmtrionla.l concentrations: Negative control, 11, 22, 43.9, $7.9, 176, and 351 mg/L plus 351 m/l abiotic Measured concentrations: <LOQ, 7.74, 15.1, 31.9, 62.5, 147, 230 mg/L; biotic control = 231 mglL Element value and 95% confidence interval (based on frond number): 33-ddaayy IICC: 1>0213m0g/mLL(C(LC1n.otnoctalccaullcaubllaeb)le) Sddaayy IICC. 30>.7230mmpg/(L13.(3C1. n1o4t2camlgc/uLl)able) SSddaayy IICC uLsi: 1>8223m0gm/LL(39(.C1.L.~2n4ot0cmalLcu)lable) dTuadayy 1IC0,<i: 221.018 mmgg//LL((415.733--124640mmga//LL)) 77-uddaayy NICOoAs:E>C23(n0umbmegrlofLf(rCo.Lndnso)t:cal1c5u.l1abmlgel)L Al clement values based on mean measured concentrations Snteacrtoitsitci,caclhlmoertothiocdsa:ndMdeeaand fprloanntdsawnedrefrcoanldcunlautmebderuss,inpge"rcMeinctrosinohfitbiEtxiocnelvaVleuressioannd5.0t7h,ewpheirlceensttaatgiestsicoalf `anaaclhysterscawtmeernetcgornoduupctaesdthuesipnegrc"enTtOrXeSduTcAtiTonVienrsmieoann3.f5r"o.ndPneurmcebnetrirnehliabtiitvieontovmaeluaens fwreorned ncaulmcbulearteidn tfhoer cpoosnstioblle,repulsiicnagtelsi.neTarheinIteCrp4o,laItCiao,n 3wnitdhIfCrovnadlnuuemsbearndan9d5e%xpcoosnufirdeecnocneceinntterravtailosnwdeartae. deTtheermpienrecde,ntwahgeens otefstdecahda,mbcehrl.oroTthice afnrdonndencrotiuc dframotnadwsbaaslseoveawleuraretecdalfcounlaotremdarleiltatyivaend10htomhogteotnaelintuyomfbvearrioafnfcreosnd(sp =in0c.a0c5h) 2 en R SE T ntae TR [NIegatiivleControl | "Al<LOQ | <LOQ 774 0 [2 sausaetss [ as1 | w | [wsTT 879 anedigsny [30 wm 76 145,146, 150, 147 ST35a1biot) |moranal2iz2ed6,,22372,52,322,5250,4357 ow-- 7 o66. 5Negative Control 1.74 Is I 25 147 230 % IT] I yy 1 ree Tt =] oF 32 1 37 I-- ENVAMRD(2002)17FINAL Effects MCeonacnenMteraatsiuorne,d DMeeaadn ChiMoeraontic | mg Fro%nds, Fron%ds, [NegativeControl| 0] ne --1 15.1 Fe Tr - 319 [1 62.5 = NeMceroatnic Fro%nds, Tar 0 023 230 Cy TN TWIR Te Cwiotnhtrtohelerxecseppotnisoen:ofsaotinsefancteocrryo.ticPlfarnotnsdaopbpseearrveedd ohenalDtahyy a3nadndexDtiabyit5eodfntohrematelstg.rowth throughout the test `iOnbcsiedrenvcaetoifonsd:eaDdu,cckhlwoereotdicexopfonseecdrottoic14fr7oanndsdd2u3r0inmgethPeFtOesSt./LBeyxhDiabyi7t,ealdldotsreea-trmeesnptongsrivoeuipn>scr3ea1s.e9 ign/tLh.e sonhotwheedwaetveirdesunrcfeaocef.sublethal effects, including root destruction and/or cupping of the plant downward CONCLUSIONS dTehteerpmoitnaesdsiumbepe1r0f8lu(c4r5o.o7c-tan1e4s4u)lmfogn/aLt.e T7h-eDa7y-DIaCy NOaAnEdC9,5b%asceodnofnidtehneceinhiinbtietrivoanl offforrodnudckpwreoedudctwiaosn and evidenceofsublethal effects, was 15.1 mg/L. `Submitter: 3M Company, EnvironmentalLaboratory,P.O,Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch rankin=g | REFERENCES This study was conducted at Wildife Intemational Lid, Easton, MID at the requestofthe 3M Company. oTHER Last changed: 7/19/01 15 ENVAMRDE002)17FINAL (RNoabvuisetulSatpuedlycuRleopsoar)t Reference No. 38 - PFOS: A 96-hour toxicity test with freshwater diatom TEST SUBSTANCE ud e1n1.t2i2t.y3:.3P4r:f4l5u.o5r6o.o6c1t.a7n.8e,s8ufoSn-ahetpetamdaecyalallisoorboe- preofaesrseidutmosaasl,PCRAOSS#or27F9C5--9359..3)(1-Octanesolfone acid, SRaemmpalreksw:asSasmtporleed oubntdaeirneadmbfireonmt3cMondpirtoidouncstiporinorlt1ntuemstbiengr. 2P1u7r.ityThdeetteersmtisnuebdsttaoncbee i8s6a.9w%hibtye pLoCw/dMeSr,, HHMR, "F-NMR and elemental analyses techniques. METHOD "MTeestth:oAdc:utOePsPTaSc850.5400 GYLeaPr:cVoemspleted: 2001 SSpoeucricees;: ONraivgiicnualllaypefrloimulTohseaCulture CollectionofAlga at the University of Texas at Austin, maintained AinnacluylttiurcealmmeodniiutmoraitngW:ilPFdOeSInmteeamsautrieodnaalt L0,d7,2,Ea9s6t-ohno,urMsD EExlpeomseuntrebapseirsi:odR:ep9o6r-theodurtshree ways: numberofcelarelaund,er the growth curve and growth rate EStnadrtddaattee::222295//0000 m"eesdtiuormgaintihsmssillicaaboarnadtosreylecnuilutmurfeo:rAaltglaeeasctulttwuoreswhkasd bpeerniacttoivteeslty ngirotwiionng,inSftroecskhwnautterrieanltgaslolcuutlitounrse "wTeerset Cpornedpiatrieodnbsy:adding reagent-grade chemicals to reverse osmosi-purified well water. `GrowCthommpeoduinudm Nowinal Tos Concentration = [CTa T 4c 40 h TT mo er | [HBMnCZO lac4,11T 0 003421 l86 8 | me6 00 r | [Coch taso|rupro erL | 0012 gL MgNSaON.O, | 1470| my K;HPO, | os mgr | [CNaco,T7150 | mel | 200 o 0.010 2 5s ENVIMRD(002)1 FINAL Dteisltutmieodniwuamtewrassouprrceep:arWeidldbliyfeadIdnitnemgattihoenaalppLrdo.priwaetlel wvaotleurmepsurioffiesdtobcykrenvuterriseentosmsoosliust.ionsThteo NpuarOiHfi.edTwheellmweadtieur.mTwhaespsHeiozfetdhebmyefdiiltuamtiownas(0a.d2j2u4smte)dp0rio7r.to5 +us0e..1 using 10% HCland0.1 N t"eTsetstsoolluuttiionosn wperreepaprraetpiaorne:dAinparligmaalrmyesdtiocukmsaotluctaiconh woafsthneosepvreenpanroemdifnoarlthciosncsteundtyr.atiIonndsi.vidTuhael finidniavitdesutalsotleusttiosnosluatpipoensarweedrcelsetairarnedd wcoiltohrlaessm.agnetic tir plate for approximately 24 hours, All cEoxnptoasinuirneg 1v0es0semlLs:ofStteersitlesol2ut5i0on,mL plastic Erlenmeyer flasks plugged with foam stoppers ANguimtabteirono:fSrehpalkiecantecso:nttihnrueeo.usly at~100 pm NInuitmibalearlgoaflccoelnlcelnotardiantgi:ons1:.s0ev1e0n*pcleulslsamnLegative contol plus an abiotic control at the highest c`oWnacteenrtrcahteimoinsttersyt:edpH range (0-96hours) 7735--787 ((c3o3n5tmol/leexxpopsousruer)e) Test temp2e3ra1t-u2r4e6ranCge (0 - 96 hours) Light levels: (0-96 hours) Methodof3ca9l-c1u4la50t1i0ngumxefarnommecoanstuirneudoucsocnocoeln-twrhaittieofnl:uoarreistchemnettilicghmteiangn obtained using results obtained at O-hours, 72-hours and 96-hours RESULTS aNboimotiincaclontcroonl,centrations: Negative control, 61.5, 813, 110, 147, 195, 264, 347 mg/L plus 347 mglL Measured concentrations: <L0Q, 623, 83.2, 111, 150, 206, 266, 335 mg/L: abiotic control = 339 mg/L E2dlcehmoeunrtEvaClu(ece(l9l5de%nsciotnyf)i=de2nc1e(i2nt1e4rv-a3l1):2) mg/L. 2244--hhoouurrEEIBCeC((garroewatuhndreatre)cu=r2ve7)9=(221622-3(20065)- m30L8.) mL. 4488--hhoouurr EEBCC.(c(ealrledaeunnsdietryc=)ur2v6e1) (= 2259-13(029627) -m30L3) mg/L 4782--hhoouurr EEXCC,.((ceglrlodwtehnrsai=t=t<ey269)2)4.3(2(C7L1.-3no0t7c)almcgu/lLable)mg/L 7722--hhoouurr EEbxCCioo((garroeawutnhdreatrec)u=rv2e2)1=(<1692.03 +(25C2I).mng/oLc.alculable) mg/L 7722--hhoouurr EEBCC,.(c(ealrledaeunsnidteyr)c=ur2v4e2) =(2204-06 (27261)-0m27L7) mg/L 7722--hhoouurr EECCana(c(eglrlodwetnhsiattye))==321975(3(03868 --332065))mmgg/LL 7722--hhoouurrEErBCCa (agrreoawtuhndreartceu=)rv3e3)5=(332138-(3350)-7m32L5) mg/L 9966--hhoouurr EECBC,ce(lrleadeunnsidteyr)c=ur<v62e.)3 (=C<.612..3 (nCot1.canlcoutlcaabllceu)lmagb/lLe.) mg/L 9966-hhoouur EErCC,0((cgerlodwtehnrsai=t=t2ye264)3)3((321079-.229995))mmgg//LL 16 ENVAMRDQOO2)I FINAL 96choue EBC (rca undecurse) = 252 (220-285)mol. 96-hour ErCyo (growth rat=e30)5 (295 - 316) mg/L. 7999662--hhhooo6uuurrrENEEXhCCOBaNnCE(cg(Cerrlloc(wcadetuelhnnddsese)ic=eut-3ry25sa)3n2)r5e(33u31min10d9-pe3rG2,0t8(hC8)e1.3umg2nv/6tLe.):cmg<a6r2.3em)el 9762--hhoouurr NNOOAAEECC ((gerlowdtehnsiatey)):: 210560mmgg.. 9966--hhoouurr NNOOAAEECC ((grreoswtuhndraet)he2c0u6vmeyg: 62.3 mg/L. AM clement values baseodn mean messed concentrations hSitenaintbisiatiliocynazlveamdleutebshyowdTeser:earcCelilenltuesrdtpeoenldsaitsiciosnn,gssi"enTcghaeTuSOnAdXSeSSTtAhyeTsgVreorfowsrtihoWnicn3ud.ro5vwetsoTMvs.asltRuiemsea,teaghr6e.o1E2w.CT.aehsEeCvaandl.nudpewrEceerCnet a9an6lduhcoLsueravsnedwne9er5et%lesco,onrafesipdelecntciueveliyft.os.TnhoeCremlealsldimetneysniattnidesg,rhooaumrpoasgsewnueenrideteyr(ohhefnevCgaorrmoipwaatnrhceeeduw1ri0entahenthdceogSnhraoopriwrwoirnWtigelDkateor7n2anatdt' Le. Resultsof he isin lysis wid 10 determine the NOREC vues. dhAiengthae.yrmtpiienarilfnogrMhmeeatnhccooednoclelinoutgiryda:_tiAocnnharolofymstahetesosgorftatpeshsutybsstowalinutctheioinmnsatswhser6esppeesrcofolotmroemnresdi,catt edWeatiemcdteieoannIdneo(rHmtPatLpoCrnoAlmSi)Ln.de.ntWwphieencsgk pirenerdscipevoinndtsueralefcioorsvoempreeyrroiffclumcooarmtopoocnaefnnocrtutsil.ffiocTnathsieioenLsOwaGanQsal(yuizsmceddi,coofnNcquuorarntteinettalmtyipodnu)rwiwanagsssm4aam3pd9leem/faoln.Qailnnyttwsyessotd1ny0.8t%h.ThSbeaamsmphelaeonsf HSCoaoumlrplslcertsadnagakedetferasotm7i92n4hto.ruor0sn r1haa0nd1ge%dmoeffarsonumrme9id.n5.vathoeF0so6rf%toemofb9ni6o.mt2i1snala.n1Mt0e6sa%,suorhfeednomameilansoauls.rfeod Maesaepsuirfenod thvheaelnsuaeam6polre fake 17 hourswas95 2% ofnominal and oth sample akeat96 hour, 96.85 ofmina. Summary of analytical chemistry daa: [Nominal Test | Measured Valuaets 0,72, snd96-| Mean | Percent of nL Concentration, hours, Respectively, mg/L ConMceeansautrieodn,| Nominal TNegatsiSveiaContrl A<T00 "ao3g Tor|| $2 [0| wTTwmia sesin is ] oe] eT261 Sa amoww Tae oiio]] 7 hioti) | Notanalyeed. 341.56 ww ENVAMRD(2002)17FINAL [BiMoelaognicMaelaosbusreerdv|ationMseaafnteNru9m6b-ehrour|s:Percent Inhibition via| Percent Ihibivion via| Percent Concentration, | 0 ofmCeLll per Density Arca Under the Curve| IGnrhoibwitthioRnavtiea 65223 Y 236.L 667ee ferr aw ed35] 2.366.667 2+ 27 Ti 150 206 [233"1 37 Tae J 266. 1.330.000 338 a TYsre "Indicateas significant difference from the negative control using Dunnet' est(p< 0.05) Control response: satisfactory Othbesefrlavsaktsiionnst:heAnfelgarl9iv6ehcouorsnotforexoapnolysurees,t ttrheraetmweenrtegrnooups.ignIsnoafdadgigtrieognatthieornewoerraedhneorennocteiocefatbhlee cahlgaanege(s0 einxpcoeslurceoalofreworcemlolsrpinhotlheog3y38wmhge/nLctormeaptamreendt g(r0outpheapnpeegaarteivdesmcaonltlroinl,coamlptahroiugshonatto7t2heacnodntr9o6l hours of RHoeuvresr.sibiTlhietytorefauGmreontwtghrouInphiwbaistiodni:lutTehdeto33a5cmongc/eLnttrraetaitomnenotf tghreoutpestwassubmstaaxnicmealtlhaytiwnohiublidtednoatfeinrhi9b6i-t roowwtth awnads efoxupnodseod bfoera7lgidsatyast.icBased on the growth observed in the recovery phase, the effect on algal CONCLUSIONS pTehlelicpulootsaasswiausmdepteerfrlmuionreodocutsainnegstuhlrfeoenactaelcu9l6a-tihoonurmetEhoCds. anBd.y9ce5l%decnosintfyi,deitncweasi2n6te3rv(a2l17f-or29N9a)vmicLul.a b96y-ahroeuaruNndOeArEthCe gwraoswtdhetceurrmvienietdwabsy 2D5u2nn(ett2's-22p5r0o)cemdpu/reLa(pnd<b0y.0g5r)owothrbaete153005mg2/9L 5u-si3n1g6)cemlgl/Ld.ensiTthye. <a6d2h.e3remngce/Lwewrheennoutseidnignaarneyaoufntdheretthesetcsuorlvuetiaonnsdo2r06thmegc/onLtrboylsg.roTwhtihs rtaetset. sNubostsaingncseowfcaesldleatgegrrmeignaetdio0n boer agit. Submitter: 311 Company, Environmental Laboratory, P.O, Box 33331,St. Paul, Minnesota, 133 DATA QUALITY Reliability: Klimisch rankin=g | REFERENCES "LTahbisRsetquudeyswtansucmobnedrucUt2e7d23a,t Wildife International Lid,Easton, MID at the requestofthe 3M Company, omer Last changed: 6/19/01 iss ENVAMRDROG)I FINAL (RSohbeulsettoSnteumdaycoRsetpaourtm)Reference No. 39 - PFOS: A 96-hour toxicity test with the marine diatom TEST SUBSTANCE I1d1e:n2t2i.t3y3:.4P:r4f,l5i.o5r.o6o.6c1t.a7n.e8s,u8lf-ohaetpetamdaeycafallsoorob-e proetfaesrseidutmosaasl,PCFAOSS#or27F9C5--9359..3)(I-Octanesufoni acid, Rdeetmearrmkinse:dtSoabmepl8e6f.ro9mb%y3MLCp/rMoSd,uct'iHoonHMltR,nu"mFb-eNrM2R17.andTchleemteensttasluabnsatlaynsceesitsecahnwihqiuteespowder, Purity METHOD "MTeestth:oAdc:utOePsTarSc850.5400 GYLeaPr:cVoemspleted: 2001 SSpoeucricees:: OSrkigeilnatlyonfrcoomsTtahteumCulture Collection of Algae and Protozoa, Dunsaffnage marine Laboratory AatnaOlbyatnicAalrgmyoln,iStcoortilnagn:d,PaFnOdSmmaienatsaiunreedadtin0c.u7l2t,ur9e6m-heoudrisuam Wilde Inerationl Ld. Eaton, MD. EElxepmoesnutrebpaesrsi:odR:ep9o6r-theodurtshee ways: numberof cell, area under th growth curv andgrowth ate SEtnadrtddaatte:e:91239/.0000 "ATneasltyotircgaalnimsomnsitloarbionrga:tToersy:ccuolntcuernet:raAtligoansemceusltsuurreesdhaatd0,be7e2,n aanctdiv9e6l-yhogurroswing in saltwater algal culure Smeaegdenit-gfroardeatclhaesmtictawlos twoeerkevserpsreioorsm0ostiesst-piunriitftiieodn.welSltwoactkern.utrSioelntutsioolnustiwoenrsewtehreenpdrielpuaterdedibyar3tdifdiicnagl Saltwater to prepare final growth media. "Test Cond`iAtlgialonssa:ltwater medivm ho -- 1 Concentration [Mnr CLa0e | TT2 c o1 mi 6 | |em mero| ZnSO TH,0 CuSOSHO| 236 2 | gt | CoHCBOL,o0 |606| ye2 NaEKDPTOA.2,0. S30e[mt 0 N"aTShiia0mi,n9e00 20020s mmeel Hydrochloride [Bon "T700 ugk5| BI2 [esTwp 1% ENVIMRD(2002)17FINAL Dviolluutmieosn rweaatgeernts-ogruardcee:chTehmeicsatloscktonuWtirlidelntifseolIunttieronnastiwoenrale Lptrde.pawreeldl bwyataedrdipnurgiftiheedabppyrorpervieartsee ososlmuotsiiosn.s tTohaertailfgiaclalmesadlituwamtewraast p3r0eppaprtedsabliyniatyd.dinTgheappprHoproifattheevmoleudmieusmowfatshe8.s1toacnkdnuittriweanst sTteesrtiliszoeldubtiyonflprraetpiaorna(t0io2n0:mA) psriinogrle0tuesse.solution (3.46 me/L) was prepared for this study in atligmaledsawlittwhataermamgendeituimc. sirThpleatseolfuotrioanppwraoxsimsaotneilcyate4d3 fhoorursa.pprTohxiemaftineallyte3s0t smoilnuuttieons aapnpdeawraesd cElxepaorsnudrecolvoerslseeslss.: Sterile 250 mL glass Erlenmeyer flasks plugged with foam stoppers cAognittaaitniionng: 1S0h0amkeLnocfoensttinsouloutuisoanl.yt 100 sm NInuitmibalearlogfalreepelliclaotaedsi:ngs:ix.7.7 X 10"cellsmL cNounmcebnetrratoifoncoenscteendtrations: one plus a negative contol plus an abiotic control at the highest `Water chemist8ry0:-p8H4ra(cnognetr(o0l-e9xp6oshuoruer)s) "Test temperatu5r0e-r8an4ge(3(200 mg/Lexposure) 96 hours) Light levels: (020296 h2o1ur4s)C Photaperiod: 31848-0ho-u4r71ig0htluaxnfdro1m0 choooulr-swdhairtke fluorescent lighting oMbeitahinoeddoaftc-ahlocuurlsa,ti7n2g-hmoeurasnamneda9s6u-rheodurcsoncentrations: arithmetic mean obtained using results RESULTS > - `thNeomhiinghaelstcocnocnecnetnrtartaitoinosno:NfegPaRtOiSveactoanitnraobll,e 3in.t4h6ismgs/alLtwpaltuesr3a.l4g6almmge/diLa.abiotic contol. This is apparerily EMleeamseunrtedvaclounece(n9t5ra%ticoonnsf:id<eLnOceQ,int3e.r2v0alm)g:/L; abiotic contro=l 3.18 mg/L c7a2lcaunldab9l6e-)hour EC via cll density, ares under the curve and growth ate: > 3.20 mg/L (C.L. not 24,45, 72, Caleulible) and 96-hEoCguviracell density, area unthedcurevearndgrowthrae: >3.20 mL. (C.L. not c7a2lcaunldab9l6e-)hour ECoviacell density, area under the curve and growth rate: > 320 mg/L (CL. not 9762--hhoouurr NNOOAAEECC(c(ceellllddeennssiittyy,, aarreeaa uunnddeerr tthhee ccuurrvve,, ggrroowwtthh raattee)):: 33.2200 mmgg//LL Al element values based on mean measured concentrations Sitnahtiibsittiicoanlvamleutehsodwesr:e Ccealllculdaetnseidtiuessi,ngar"eTaheunSdAerS tShyestgermowftorh Wciunrdvoewsv"al,ueRse,legarsoew6t.h12.raTtehseaEndC,peErcCean,t adenndsitFieCs., avraelauseusndaenrdt9he5g%rocwotnhficduernvceealnidmigtsrocwothldrantostatbe72caalncdul9a6tehdouurssinwgersetteivsailcuaaltemdetfhorodnso.rmalCiotly cusoimnpgartheedSthoatphieroc-oWntirlokl'sustienstgaAnNdOfoVrAequaanlditayo2f-vsaarmpilaencLetsuts.ing an F-test. The treatment groups were then 190 ENVIMRDQO)I FINAL Results ofthe satisial analyses were sed to determine the NOAEC values. AhHionguahrytpSieacmsapotlmeMasentwcheeordelolicoedgmyrc:ihurAgonemadaltyaospgeprsraoopxfhiyemsaitttehsloymlua1ts0isomnssipmweiesrccesopameercafpeorpemeevcdiamstoarnWliy(fH2P0e0L0CeA1rSpa)mt.iproTionrhst7oL3iac.amnduysi.ng paWrhioesmninoefdneittnedpirevmaiikdnuiranelgspmioehnesreicfoocncceponemirpafotvnireonontosco:tfantTcheseultLisoOnGtseu(bwisatmasincuoesfd.q1uatNnhoteitateiemtonpsswlamwisaos0n.sm4,5d0tehm"0fs.qamue tinheiosnpyo Tm1eh0ae3s%um.reeSadanmvppalelerucseenfctoorlrleehccetoevdsearamytploteefstmtanakitentiiaotfonr7t2ihfaihdcoautmisoensswasasenad3ly2vz.ae2ld%uecosofnrncooummrien0an6ll..y2 Td1huen8gm5e5ss%asoemfdpnovnmaianlaevl.eforTwthhdee SSaammppllee atkakeenatat7926hohuorusrswawsa9s7.91152o%fnoofminnomailnaal.d orFohretsheamaplieoakceonato9l, htohuersm,ea86s.5uredofvnoonf,o the SuNmommairnayloTfeasnaltyti|cal | Comenaion| MecahseumriesdtVrayludetsa:0 72, nd 9.| hous, Respectively mph. | MeMweuarned || PNeorcmeinntalof mL Concpemaytion, NegaSiniesCon] 3A26I.S31L90.3005 Tat bio) | Notanaljed, 336.500 3t0og |_o_z|] Sov] Biological abseryations afer 96hours |[MCeonacnemnMetetraatsiuorne,d|| MoefaCnemNlLulmabrer||nbiDPbeeirnciseotnntyv| is| nAirbPcieartUcoennndtveira|| IGnrPhoiewbrtuchieonRntavtiea LNegate Conol3aGer |~ he Cu~ re aT ese Contra response: stisfctory niOhebegsaeTtriavvsaektsicooinnnst:tohlAe fgtrrroeua9pt.6mehnTothuergrsreooufewp.exrpeoHsnuoorween,voettrihceehrarbwlseerewchenaronegsesisgingsinnofoefalgardemhgoearrtpeihnoconeloo1groyahdWebheeernesnccocomhfpatamrbheedrltigon tth0he negative conto. rResoerwsiibnilhtey HoifghGerstowcotnhceTntariabtiiionons:teAdi(r39260-mhgou)r.oTfheexepfots,e,4tchoevrerwayspnhoesiwgansifnicsantcoinnidseiino.n of coxcLusions 1 ScAoasasalitnumgemlde.iac.o"nTcheTisnhtemrea9ta6in-ohnmoeouarfspEuorCteadssacinoudnmc9ep5ner%faltcuooonnr,todo3ec2nt0caenmersyua.folnwtaefsowhaeSskheeixglhacelosttneecdomnccfeoontntroaaxtniic,ointy2attdoacnSeakrbmllieenoienndhebimye erNleOlsAduEennCsdietwrya.hseadrestueurrnemdie4nrneddheEbrygorAwotNwhtOahVtecA,urNavo,naiaingd2n-bayomfgparlloelwt4Sh 5rBt10FwbaesF3fo.au30nAdimdehtloeo.rbeesnn-ateu3wl2et0redmroLen.idng Tcehaln9yd6eofhnoytu.hr et solutions or te conto. ENVAMRDQOO)IFINAL `Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331,St.Paul,Minnesots, $5133 DATA QUALITY Reliability: Klinisch rankin=g g er REFERENCES "LhaibsRsetquudeystwausmbcoenrduUc2t7ed23at WiKIe Totemationl Ld, Easton, MDa the request of the 3M Company. otHER Last changed: 719001 10 ENVAMRD(2002)17FINAL (RoFbEuTsAtX)Study Report Reference No. 40 - PFOS: A frog embryo teratogenesis assay ~ Xenopus TEST SUBSTANCE aIcdiedn.ti1t1y:22P.e3r.f3lu4o4r.o5o5c,t6a.ne6s1u.l7f,o8n8at5e-;hempatyadaelcsaofbleuorreof-e,repdottoaasssiPumROsSa,, UC2A7S23#2o7r9F5C.-3995..3)(1-Octanesulfonic PRuermiatyrkdset:erSmaimnpeldetoobbetai8n6ed.f9bro%ym L3CM/MpSr,od'ucHt-iHoMnRl,ot"nFu-mNbeMrR2a1n7.d eTlehmeentteatlssunbasltyasnecseteicshaniwqhuietse. powder. METHOD _ MTeestthotdyp:e:AStSaTtiMc rEe1n4e3w9a.l91 Y(GeLaPr:cIonmfpilfeetpehda:se2-00n1o tock solution preparation and messurement oftest concentration-s yes Numberofstudies: 3 Stayt Swdy2 Swdyd EStnadrtddaattee:: 5S1I90s0 5S0260000 5522620000 A(nSatluydtyi2caalnmdon3istetoupricnogn:cuPrFrOenStmlyewitah casotm0muaonndrs9t6oe-chkosduorlsutions) S`Sopuercciees:: XBerneoepduisnglaecvoilsonies at the University of Maryland Wye Research and Education Center "(TUesMtDo/rWgRaEnCi)s,msQluaebeonrsattoowrny,cMulatruyrlea:nd.Mating pais were bred in the dark in 23.5 + 05C UMDWREC ononna-dcohtlroroipniante(dHCweGl)l iwnattheerdoartsa~l 7l0ymdpahysacinotefrtvahles mbayleisnjaencdtifnegma4l0e0s,arnedsp8ec0t0ive1.lyU.. Aomfplheuxmuasnocchcoruironeidc A6gehoautrsteasftteirniitnijaetcitoinn:gEHmCbGr;yeosg;gndoerpmoaslitsion oa8ccbulgarsetduel9-t102 nhoorurmsalfosltalgoewi1n1ggHaCstGrulianjection. PLroeatdrienagt:me2n5te:mbErmyborsy/o1s0 dmeLj.elled in a 2% L cysteine solution, then rinsed and re-suspended in FETAX Esolleumtieonntpbraisoirs0: mionrttraoldiutcyt,imonaltfoortemsattcihoanmsbe(rvsi.th ala of Bante et al, 1991), growth Exposure period: 96-hours "Test CondDiitliuotnison(awlalt3er:stuAdSieTsM): (1998) FETAX solution TLeisgthttelemvpeelrs:at6u0r-e5:5 2f4o.o0t c+a0n.d2leCfluorescent lights SPthooctkopaenrdiotde:s1t2s-ohloutuirolnigphrtepa1r2a-thioounrd:aArkprimary stock solution was prepared in FETAX medium s(osluuptpiloinedwbays UmMix-eWdRbEyC)sonbiycaWtiilondiafnedIsntteimnagt.ionaAlf,teLrid.miaxtin4g8,mtghePpFrOiSmLa.ry sTthoeckpsroilmuatriyonstwoacsk cproonpcoernttiroantailolnysdoilluuttieodnswwietrheFdEelTiAveXremdetdoiUuMm-1W0 RprEeCparperiotrhe( sitxhetsettarctoonfeceantcrhatsitoundsy.. Thesi test SRteofcekreanncde rseufbesrteannccee:su6b-satmainnocneiscooltuitniaomnipdreeparation: as outlined in the ASTM (1998) protocol Exposure vessels: Covered 60 mm glass Petr dishes containing 10 mL test solution 103 ENVAMRD(002)17FINAL NNuummbbeerroofferempblricyaotsesp:ecronrterpolliscate4:, t2r5eatments 2 Ncounmcebnetrratoifoncotenscteendt,rpaltuisontsw:osrexfeprleunsceasunbesgtaatinvcee ccoonncternotlraptliuosns.an abiotic control at the highest RSteanbeilwiatlyoffrtehqeuteenscty:cehveemriyca2l4shooluurtsions: Extremely stable Water chePmHirsatnrgyed(ur0i-n9g6ahlolu3rsst)udies: 770.71.76.7 ((c2o4ntmrgo/lLexnpoomsiunrael)exposure) D7.3isso8l.4vedmoxLyg(ecnonrtaonlgeex(p0os-u9reh)ours) Methodof7.c0alc8u.l5atmign/gLm(e2a4nmgme/aLsnuormeidnaclonecxepnotsruartei)ons: arithmetic mean RESULTS limi `Nmogm/iLnaabliotPiFc OcoSntcroolncentrations: Negative contol, 1.82, 3.07, 5.19, 864, 14.4 and 24.0 mg/L plus 24.0 NMoemainnamlea-saumriendonPiFcoOtSincoanmciednetrcaotnicoennst:rations: 5.5 and 2500 mg/L Sabtiuodtiy1c :con<tLro0lQ-, 200, 283, 23.7 mg/L 4.73, 7.90, 147, 24.6 mL; aSbtiuodtiyc2c:on<tLroOlQ~,23.19.91,mg3./0L4, 482, 7.97, 133, 23.0 mglL; Stabuidotyic3c:on<tLro0lQ=.241.913,m3g2L7, 525,826,140, 23.9 mg/L; PFOSSucdlyem|ent v9al6uHesraLnCda9|5% c9o6nfHirdEenCcse i|nterMvailsn,immugm/cLonc to] Teraogenic } Number InhibMitCGIr)owth | Index (TT) T | BEis3y 146) Notcalculable TT i Tess200)] me29a) s 757 - To S3 I B33 2 26 [x All clement values based on mean measuredconcentrations hStoautrisLtiCc.alfomremtohrotdasl:itTyhaendTr9i6m-hmoeudr SEpCesarmfoarn-maKlafrobremratsitoantsis.ticTalheprMocCeIduGrewawsasdeutseerdmitnoeddebtyerBmoinnfeertrhoen9i6'-s T-Test. All statistical tests were performed using Toxstat (WEST and Gulley, 1994). A minimum pECrobably level of 0.05 was used. The teratogenic index (TT) was calculatedby dividing the LCx by the AMnDalyutsiicnagl Mheigthhopdeorlfoogrym:anAcneallyisqeusiodfctherstomsaotlougtiroanpshwyerweitphermafsosrmseapedtctWriolmdeltirfeicIndteetrencattiioonnal (LHidP,LCE/aMsSo)n., Wprhoemninednettepremaiknirnegsptohnesefcoonrcpeenrtfrlautoironoocotfantehseultfeosrtatseubwsatasncuseedi.n tNhoe attetsetmpstolwutaisonms,adtehetosqaumaentiafnydonmotshte 194 ENVAM/RD(00)1 FINAL bTahseismoefainndipveirdcueanltirseocmoevreircycoomfpomnaternitxs..frTthiefiLcaOiQon(sliamniatolyfzqeudanctointcautrirone)ntwlaysd0u.r2i4n0gmsga/mLplien tahnedsleyssiusdiwcass. s9t7u.d2y.%aSnadmipnlethsecsolelceocntdedaandetshtirdntsttuidoisn, hfaodmme95a.8surteod 1v1al7u%esoffrnoommi1n1a2l.toM1e4as1u%roefdnvoamliunesalfsoianmthpelrets htaekesnecaton9d6-ahnodurtshirradnsgteuddiefs.omT$h4e.7s1a0mp98l.6e%s froofm ntohmianbaioltiicn t2h4e.0frmsgt/sLturdcyaamnedn8t0.7gro1u.p1w1a2s%coofmpnaormaibnlael 1i0n samples from he 24.0 mL. treatment group with he embryos present. Summary ofanalytical chemistry data: Stud1y ConcNeonmtriantailonTe,stm_|g/L| MehaosuurrseRdeVspecativlaelty0u,amnegd/9Ls6-|| MCeonacnenVtireaatsiuorne,d|| PNeorcmeinntalof |NegativeConwol|"AW<LOQ | clog | | 258,142 1 2.00 Tie so 8.64. lad 240. [240G@biotic) seam an oi | [i=o7r 0 [o s a| 18.5, 108 269.223 a6 T1103 [TM notanalyzed,337 |237 | 986 | StudyNo2minal Test | MeasuredValues a030d96-| Mean Measured| Percent of Concentration, mg/L. Respectoivuerlsy, mL Concemntrgation,| Nominal Negative Conwol | AN<LOQ |<0Q | | 191 105. 3.04 99.0 [sv TT sasas Tas |oe | set 1"soy: 1907 Tw | [a1 esr T133 9a 1| 240 1 23.0,23.1 963 [240Gbiotic)|"notanalyzed 39 | 239 | 996 Sudy3 ConcNeonmtirantailonT,esmtg/L| MeasuredValouuerssa030 96-| MCeonacnenMtersastuiorne,d|| PNeormceinntalof Respectively, mg/L mpl [NegativeConwol| AW<L0Q | <toQq | | [ie Tim tes a | e6 | [er 5.19 1s5o 45.5a 05 | 320 | wer | 8.64. 8.43,8.09 [a 240 777 e25s0.i20s7 240 abiotic) otanalyzed, 24.1 a290 1 o9r6a | 241 00 195 Test3 Ere EEE Negative Control1].0 |4 Se. IT 3 182 o 180 To[60 | [ToorTaoTis T00 T74 094 0 | 864 25 --3 [CaTs T7765 1 30 | 57 |aa |""3 | rer re epee em eee Lot ootLor} [Negative Control |__859 |__888 |oar| [as T 1 s sds 1 ei | [oor T7880 1 857 oa | [ssiee1 en ssi 1"e8nw_[|w92s8 ]| [a0 |m:Gootal5r.08tali7t.3y9)% _,|___ E7.5T0% Iph ENVAMRD(2002)1 FINAL Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesoia, 55133 DATA QUALITY. RAelltihaobuilgihtyt:heKsleimwiesrcehwrealnlkcoinndg=uc2ted studies, the inife phases were not conducted in acsordance with Good Laboratory Practices REFERENCES WThReEsCe)stuindiQeuseweenrsetocwonn,duMcatreydlaantdtahnedUnaitveWrisiltdy iofIMntaermyaltainodnaWlyLed.ReEsaesatrocnh, ManDd aEtdutchaerteiqouneCsetnotfetrh(eU3MMCompany. Lab Request number U2723 OTHER Last changed: 7/19/01 17 ENVAMRD(002)17FINAL tRhorbouusgthSbtiuodcyonRceepnotrrattiRoenfetresetncweithNot.he41Bl-uePgeirfll(uLoerpooocmtiasnemsauclrfoocnhaitreu,s)Potassium sat (PFOS): A flow- TEST SUBSTANCE I1d1e2nt.i2t.y3:3,4P:e4rf,l5u5o6r6o,oc1t.a7n.e8s.u8lfSohneapttea;dmecaayflavlosorob,eproetfaersrseidumtosaalst,PCFAOSS#o2r7F9C5-.9359.-3)(1-Octancsulfonic acid, dReemtaerkrsm:itnoSebamdepl8e6.f9r%omby3LMC/pMrSod,uc'tHi-oHnMfRo,t n"uFm-bNerM2R17a.nd cTlheemetnetsatlsaunbasltyasnecsetieschaniwqhuiets.e powder. Purity METHOD: - "MTeytpheo:d/Flgouwi-dtehlrionueghfoelxlpoowsedu:reUwSitEhPlAowO-PhPrToSug8h50d.e1pu7r3a0tiaonndpOhaEsCe.D 305 YGeLaPr:(2V0I0N0): Yes ``SSpuepcpileise:r:BlOuseagiglelC(aLtefpsohmeriiscsm,acInrc.o,cOisrausg)e Beach, Missouri raLnegnget2h0a3n-d3w3ei2ght at test termination: Mean length = 62 mm, range 56-66 mm Mean weight = 2.70 g, oLfoathdeinsgt:udy0and.gvfiosl4hu/mLeio8dfawya(tbearsetdhoatnpainsisteiadltlhoraoduignhgotfes9t0chfiasmhbeprerinta2n4k-,huosuirn)g. mean fish weighta the end AFniaslhyatgiec:alApmopnriotxoirminagt:elC7yonmcoennttrhastiaoneosfnPiFtiOaStiionnwater and fish. NPruemtbreeartomfencto:nceNnotnreations: Two plus a negative contol UTpesttakcoenpceernitorda:tions (me6a2-ndmayesas(u0r.e0d86):mgNe/gLateixvpeocsounrter)ol,0.056and0.87 mg/L m3o5r-adlaiytsy)(0.87 mL exposure-- his exposure ended after 35-days due to fish Depuration period:~~SN6o-ndeay(s0.(008.708m6gm/L/eLxepxopsousreu)re "Test condiDtiilountsi:on water: Moderately hard well water Dilution wSapetceirficchecmoinsdturcyt:ance: 313 (310 -315 ymhosiem) AHlakradlnienistsy::113708((112756-- 113728)mg/L) MPeHa:su8r1ed(8du0r-in8g2t)he 4 week period immediatly preceding the est, iStLoc.k aSntodcktesstolustoilountsiosnirperedpawirtahtiaonnselTecwtroicsttoopck-dsoowlnutmionis xwe0ereirdprienpatrheedsoaltub1i0liazantdion10o0f tmhge atepsptrosuxbismtaatnecel.y wAefetkelrymiinxtienrvga,lsthdeursitnogcktsheapuppetaarkeedpchalseea.r anSdtoccoklsorilensjse.ctedStionctkosthweerdeilpureerpamriexdinagt mchLa/mmbienrusteat10a sactheieoveft3h.5emdLeismiirendutteetwchoenrceentthreaytiwoensr.e mAillxefdinawlitthestdisloultuitoinonwsaatpepeataraedatceleoafr a3n5d0 cDoillourtleesrsf.low rate: Appros. 6.3 volume additions per 24-hours 108 ENVAMRD(2002)17FINAL ENxupmobseurreofversespellisc:at1e0s4:LsNtoanienleosnseseveeslsaelqpuaerricaofniclelnedtrwaittihoanpproximately 80Lsolution. D`iNeutm:beFrlaokfeifsohodp,eZrievgelseserlB:ro9t0hers, Inc, Gardners, PA `Water chemistry ranges during etshe.sCtoundvye:r | 006mg | 05m 2688--8260 ||6 2178 -20- | 68 4-86 3 | pH: 79-82 79-82 PLhiogthotpienrtieonsdi:ty1:6h2o7u8rsluxlaightsuarnfdacehooufrtshedanergkawtiivtehcaon3t0r-omlivneustseeltraatnesisttionnipteriioodn. Collection of points during tthiess6u2e-dsaaympulpetsa:keFpihsahsew.erTehceoylwleecrteeeduftrhoamnitzeesdt,chblaomtbteedrsdrby,y wreaingdhoemd saenldecmteiaosnurated.12 tFiimseh "tThheen frriancsteidonwsitwhedrieluitnidoinviwdautaelrl,ybwleoitgehdedd.ry aTghaeinhaeandd,difisnssecatnedd ivnisoceerdaibwleeraendconnosni-dcedriebdletoisbseuenofrna-cdtiiobnsl.e tissue. The remaining issue, including skin was considered o be edible tissue Sntoanteidsitbiclaelpamretst.hodSst:eadWyh-sotlaetefbiishococnocnecnetntrraattiioonnsBCwFerevalcuaelsculcaatlecdulabtaesdedfroonm tthhee stiusmsueocfontcheenterdaitbiloensanadt apparent steady-state divided by the mean water concentration. Tissue concentrations were considered (0 dbieffateraepnpa(rpen>t0s.t0e5)a.dy-sTtiastseueifc3onocrenmtorrateiocnosnsweecruetiveevasleutasteodf ftoirssnuoercmoanlcietnytraantdiohnsomWoegreeneniottysoigfnivfairciaanntcley dsautiangwtahsetSrhaanpsifroor-mWeidlki'nsatnesatttaenmdptBa1r0ectotr'rsecttetth, rdeastpae.ctiMveelay.n tIifstshuee cdoantcaendtirdatniootnsmeweetrethethaesnscuommppiaornesd, using ANOVA and Dunnett's test. tThheeekdiinbeltei,cnboinoecdoinbcleentarnadtownhoflacetofris(hBeCxFpKo)s,eduptioak0e.0r8t6e m(ek/)LanPdFOdeSpuursaitnigonBrIaOtF(AkyC) cwoemrpeuctaelrcusloafttewdarfeo.r tBiImOe-FcAoCnceisntaranotnolnidnaetaar. paTrhaemseetrearteesctoinmsattaenrtosuwteinreewthhiecnhusesdtitmoactesaatle ccaonBustCaFlntKsaf(rBtoCmFaeK s=etKo/fKseq1u2)e,ntial RESULTS _ NMoemainnamleacsonucreendtrcaotnicoennst:raNteigoantsi:ve<c0o.0n5t,r0o.l0806.1 aanndd 01.0.m87gm/LL Bioconcentration factors (BF): 0.086 mg/L apparent steady-state BCE Edible 484 856 0.87 mg/L. (study ended prior to achieving steady-state)BCF: Edible Whole Fish 136 386. 199 ENVAMRDQOO2)I FINAL BIOFAC Estimates (sing 1.086 mL. exposure) [Bem 1is66 |a2 | 361 | ane PVRaOlSuaeCrs oomnncd ienTnishsauttesorsfcBlhsuaeagmitlplElxieposoeero. n00.s086 mgt. aha po nie C0T 4-hours) 1070.31667,,007.12565,.00.610440,,0.0188026T 166085.172,207|0.21933,60.,31513,4.0.122869,.01.5363 3 7 102,106, 119,152 321,4.04,4.18,438 7.33,7.66,8.73, 11.4. F 14 273,232,353,326 202.169.260.246 I 21 | 28 49.4,30.7, 653, 57.9 35.3,292,45.4,44.1 --=35. 276.255.212.076 | 667110,675313,652404..7599.61 | 456031,.543984,. 4460.65,546039 29 | 333.362,39.0,306 62 |DepurtionbayT_T1 [=14 17204,57.914.,881587,.914135 a C36 17211,|35777 .806 3.8, 54.83 44| 249 1.6,. 6653 .6581 .62.2 1 | PVaFlOuSeCroencfenrtormatiinodnisviidnuaTl ishsaotsefxBuchlueseagsmlplEexppeorsieodd.10 037 mg. DoUptake: mals `EdibleTissue, x `Non-edible Tissue, mg 0(d-hours) 3 468.659.556.564 |111,142,133,121 | 800.109.102.047 | [ 7 749,770,853, 89.8 14 21 141,157, 180, 158 Sampli28ng of Th snp10a2,fe1r31U,pt1a0k7,e D13a3y 38 dusTo meri Test orgaNneigsamtimvoertcaolnittryo:l: None 0.086 my. exporares Odnaeinfhtheduepdskaerph4as9ed(y62sdandyoornedseaper)o59ndpohasseo(f3e5 xdapysa)n in the upp0t8ha7kaemaatplw.hhipcohanstouisermedee:uallnM,fograhtlhietHdyheiphsardnoconhtpriansdeiDd oa9r haynddbceonntisnaumepdletdhrough Day3 ofthe uptake Analytical methodology: Analyses of test soins and fh tissues were performed at Willie 20 ENVAMRDEOO2)ITFINAL Isnpteecmtartoimoentarli,c Ldiedt,etiWona.teTissasmupelessamwpelerse wdeilruetehdomaongdenainzaelydz,edexbtayctHePd,LCitweitdh asinndglaenaqluyazdeedubpyoeHmPaLsCs Sibtthantrciepleinqutahdersuapmoplleesm,atshsesspaemcteroamnedtrmiocstdetpercotmioinn.entWpheeank, rdeestpeornmsienifnogr ptheeflcoornocoencttraantciaounolffontthee wtaesst 0i.e0d5 mNLo.afottemwpattewaisn mthaidsesttuodyq.uanFtoirfyisosnuethseambpalseiss,otfheinLdOivQidwuasiscoamleeruilcactdomapnanaenntisn.divTidhuealLbOaQsswfaosr ctoacchonSsatmanptlewesiignhcte, ach entire submited sample, of difering weight, was extracted without an adjustment RAneacloyvteircaylwraessuletxscewlelreentnoitn cboortrhewcatetderfranpdroicehduriaslsureesc,ovrearnyg.ing from 84.9 10 122% of fortification levels conctusions ParRsOiSnebiooncoDncaeynt4r9atoerd itnhetfhieshtiesxspuoesseodf1b0lu0e.g0i8l6l msugnfaisLh.durAinlglotuhigshsDudayy.49,Ap6paraenndt62stetasduy-estraetseidwuaess `iwmeer.e nAoppsatrateinstticsatlelaydys-isgtnaitfeicBanCtlFyviafleueesntf,orPedEiOblSe,connocne-ndtirbatlieonasndapwpheoalreedisohbetisssiuelsiwnecrreeacsailncguldautreidng0thbies 84. 1124, and $50, respectively. ePdFiOblSe adnedpuwrhaotlede fsilsohwliys.sueTshweerBeIO14F6A,C13e3satinmdat1e5s2 dfoaryt,heretsipmeect1i0vere.ach 50% clearance for edible, non DATA QUALITY Reliabiliy: Kiimisch ranking = 1 REFERENCES TLhaibsRseucdueystwansumcboenrduUct2e7d23a Wildife erations, Lid, Eason, MD at the requestofthe 3M Company. orr Last changed: 71901 wn ENVIMRD2002)17FINAL SRtoabtuicstAcSuttuedyToRxeicpiotrytTReesftewrietnhcethNeo.Ra4i2n-boPewrTfrluoourtoo(cOtnacnoershuylfnocnhautse,myPkoitsass)siinumfrseaslhtw(aPtFeOrS): 96-Hour TESTSUBSTANCE ~ A Identity: Remarks: PFeCr.f9l5u.o(r1o-oOccttaanenseusluflofnoantiec; macaidy.a1ls,o1b2e.2re3f,e3r,e4d40.a5s.5P.F6O,S86.o7r8.81 Tehpetatdeestcasfulbusotraon-c,e pisotaawshsiituempsaolwtd,eCr.ASSa#m2p7l9e5.w3a9s.3ta)ken from 3teMstinlgo.t nPuurmibteyrd2e1t7e.rmSianmepdlteobweas8s6t.o9r%edbuyndLeCr/MamSb,ie|nHt-cHonMdRit,io1n9sFpr-iNorMR and elemental analyses techniques. METHODee ------------------------------ "MTeytpheod: OStPatPiTcSac8u5t0e.1075 and OECD 203 GDaLt:e completed:Yes Study completed 2001, report completed 2002 SSuppepcileise:r OTnhcoomrahsynFcishhusCommypkiasnsy, Anderson, CA EAxnaploystuircealpmeroinoidt:oring: P96O-hSoumresasured at 0, 45, 96-hours Statistical methods: LanCaSly0sivsa,lmueosvicnalgualvaetreda,gewmhenepotsosribhbilneoobmyidaprlopbirtobability with nonlinear Test fish age: jiunvteenripolleastion using the computer softwareofCE. Stephan. LoAavdeirangge:Total Length a0n2d3wgeifghits:h3.6 (3.4-4.0) em, 034 (025.047) PrTeetstrecaontdmietinotns: None DDiilluuttiioonn wwaatteerr:chemistr0y.4(5duurmingfitltheeredd-mwoedeekrapteerliyohdard well water ihamrmdendeisast:ely preceding t1h3e0t(e1st2)8:-132) mg/L as CaCO3 alkalinity: 177 (176-178) mg/L as CaCO3 pTiotc: 8No3t8g2iv-en84) SCotnodcukcatinvdittyes:t3s1o1lu(t1io0n-3p1re5p)arpamthioons:iePmrimary stock prepared in iuion water at 150 mg/L and mised for <23 Aofutresrpmriixoin0g,usperimary stock solution wasproportionally diluted with dilution water to prepare the five test conceniratons. CStoanbcielnittyraotftihonesdteossticnhgemriactael: soOluntcieo.ns: Extremely stable Exposure vessels a2p5prLopxoilmyaettehlyyle1n5eLoaqfuaersitascoolnuttaiionn;inwgater depth approx 17.5 cm. NNuummbbeerroofffriesphlipcaetresr:eplicate: wteon m ENV/IMRDQ00F2I)NA1L `NWateur cohmefmciosnbtcreynte druartiir nognst:he stfuidyv;eplus anegativecontrol Dissolved oxygen range (0-969ho2urs)10.8:9.m4g-/L10.(50mgm/gL/.L(ecoxnptorsoulreex)posure) PH range 8(.01-89.64hocuornst)rol exposure) "Test temperature range8(208-.496 h(o5u0rsm) L exposure) 112.11 8 - 11229.6CC ((c5o0ntmroLl exepxopsousruer)e) - Meotfchalcuolatding mean measured concentrations: arithmetic mean RESULTS -- Nominal concentrations:(Bakbioctoinct)roml,L3.1, 63, 13,25, 50 (exposure), 50 MEleeamseunrtedvacluoen:cen2t4r-ahtoiuornsL:CS<0L0=Q>,$3.00, 6m3y,(1C31,.25n,ot50c,al5c2ulmabgl/eL) 4782--hhoouurr LLCCSS00 ==> > $300 mmgg//LL ((CCLLLL.. nnoott ccaallccuullaabbllee)) A9l6l-hcoluermeLnCtSv0al=ue2s2b(a1s5ed- 2o7n)mmega/nLmeasured concentrations Statistical EvaluEalteimoenntofvMaolruetsalaintdy:confidence limits for 24,48, and 72-hours could not bcaelccuallactuelattheed9d6u-eho0urlaLCkSo0f.mortality. Probit Analysis was used fo Analytical Methodology: uAsnianlgysehisghofpteersftorsomlauntcieonsliqwueirdechpreormfaortmoegdraapthyWiwlidtihfemaIsnstemsapteicotnraolmetLriidc. sdeutbescttainocne i(nHPthLeC/teMsSt)s.oluWtihoensn, hdeetesrammienianngd mthoestcopnrcoemnitnreanttiopneaokfrtehsepotnesset forperfluorooctanesulfonate was used. Ncoompaotnteenmtpst. wTahse LmaOdQe(ltiomitqoufanqiufayntiotnatitohne) wbaassis0.o2f00imndgi/vLiduianlthiissosmuedryi.c Tduhreinmgeasanmpplereceanntalyrseicsovwerays o1f01.matSraixmpfloerstifcioclalteicotnsedanataltyezsetdicnoantciuorrnenhtaldy bmieoatsicurseadmpvlaelsuestafkernomat934.82-hfoour1s03r%angoefdnofmrionaml.93.M6eatsour1e0d3%valoufesnofmoirnatlh,e wvahliuleesafboirotbiicotsicamspalmepslersantgaekdenfartom96-1h0o5urtos. r1a0ng6e%d offronmom9i1n.a4l.fo M1ea0s5u%reodf nominal, while the abiotic samples were 102%ofnominal, 0 ENVAMRD(2002)17FINAL `Summary of analytical chemistry data: CNoonmceinntarlatTieosnt, ML a`8Measured Duplicate Values a0, |MeMaesaunred and 96 hours, Respectively, | Concentration, NPoermciennatlof [NegativeComal [ALO [dog [= [63 315,302,290301283.297 [30 1622,621,616,643,615.660 [63 lor | [100 | 1235 [ 25.03 ,25.2 7,24.2 3,251 .B7,L21 522.676,22 [13723 1[0i0o 497.98 SLLSS. 96508 [0 Tio | [s1o)GbSiamoptliees)(ta0ken at 48 and 1965-3h.o1u.r5s2o.n6l.y509.51.0 [T 2 e Biological observations after 96-hours: mFioshritnathleoinrteogivateirsvesciognntsroofl atonxdictithye.3.A0llasnudr6v.i3vimngg/fLishexipnotshuere13caonncden2t5ramtgi/onLaepxppeoasruerdesnoarpmpaelarweidthnonromal `with no overt signsof toxicity afer 6-hours. Cumulative percent mortality: TMeesatnConMee,aswmrpedl | [NegComol To To -- "To -- To | AeI Tleff I e My e jer-- 1 Er rh ETT cr TTT TeThee ae Lowest concentration causing 100% mortality: 50 mg/L. Mortality of controls: None CONCLUSIONS : a "wTihtehpo9ta5s%sicuomnpfeirdfelnucoeroiontcetravnaelsuolffo1n8a-t2e796mg-/hLo.urTLhCeS9O6-fhoorurrainnobmoorwttarloiuttywaansddnetoeerfmfeicntesdctooncbeent2a2timo/nl.was 63mg. Submitter: 3M, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 0 ENVAMRD(2002)1 FINAL DAAeee Reliability: Kiimisch rarin=g | RRC er ---- -------------------- "This studywas conduatcWtiedde Intemational Ld. Easton, MD at the request of 3M. ope LN) Last changed: 12402 20s ENVAMRD(2002)17FINAL `RSoebmui-sSttaSttiucdAycRuetpeoTrotxiRceifteyrTeensctewNiot.h4th3-e SPehreMeuposrhoeoacdtaMniesnunlofwon(aCtyep,rPiontoadsosnivumasarlti(PeFigOnSas)a:tl9tuw6a-stHeo)rur A eee tcetae remem ----------------s Identity FPCe-r9f5l.uo(r1o-oOcctuaannescusluflofnoantiec;acmiad,y 1al1s.o2b2e.r3e3f4e.re4d5,05a.s66P.R1O.S78o.r85 heptadecafluoro-, potassium salt, CAS # 2795.30.3) Remarks: 3ThMeltoetsntusmubbsetran2c1e7.isSaawmhpilteewpaoswdsetro.reSdaumnpdleerwamsbiteanktencofnrdoimtions prior to atensdtincgl.emPeunrtiatly daneatleyrsmeisnetdecthonbiqeue$s6.,9% by LC/MS, 1 H-HMR, 19 F-NMR METHOD. ete lO EE MTeytpheod: OSPtaPtiTcSre8n5e0w.a1l075 GDLaPt.e completed: Yes Study completed 2001, report completed 2002 SSuppepcliieesr: Cyprinodon varicgarus `Aquatic BioSystems, Inc, Fort Collins, CO rAneanleywtailcaatl2m4o,n4i8t,orainndg:72PhoFuOrSs amnedasautrteesdtatteirnmiitniaattiioonn, (p9r6io-rhotuorasn)d after SEtxaptiosstuircaelpmeertihodo:ds9:6-Thhoeurusse ofa single est concentration (a water S"Toelsutbifliisthy)agper:ecJluuvdeendiltehse statistical calculation ofLCSO values. LAovaedriangge:T0o.t2a9lgLeGnsgt.h and weight: 3.0 (2.4.3.5) cm, 0.44 (021-066). Pretreatment: None. Test conditions: Dilution water: oNfaatpuprraolxsiemaawtaetlery,2l0epraretds paenrdtdhioluustaedntd wiatshalwienliltywater Diimlmuetdiioantwealtyeprreccheemdiisntgrtyhe(dtuersi):ng the 4-weck period Saline: pi 8220(208-1-2803))partspr thousand PSrtiomcakraynsdtotcekstprsoelpuatrieodninprmeeptahraantoiloant: 40 mg/L, sonicated for approxim20amtineulteys snd inverted 0 mpriexpaprreiotrh0e aunsee.teAsfttceorncmeinxtirnagt,iopnr.imEaarcyhstsoocluktisoonluwtaiosnswirsedprwoporitisotntaailnllyhesdislsutteld wwihtihsdkilfuotriaopnpwraotxeirma(t0ely one minute. All test solutions appeared clear and colorless. SSoallvveenntt concentrMaettihoann(atlreatment and solvent control groups): 0.5 mU/L 206 ENVIMRDR00F2IN)AIL `Concenteations dosing rate: Daly sc renewal Stability of th test chemical solutions: Extenely sable aEpxpproosxuirmeatveelsysel1s7.: 2c5mL. polyethylene aquaria containing approximately SLoftest solution; water depth NNuummbbeerr ooffrfeipslhicpaetresr:eptlhircea(:bitoetnic), two (abiotic) Numberof concentrations: Oneplus negative ansolvdent control, and an abiotic souion. Water cDhiesmsiosltvreyddouxryignegntrhaensgteudy: (0-96 hours): 211.678-777..664 mmmeggl//LLL (((sn1oe5lgvmaetenitvleccoeonxntptoorsloulerxeep)xopsousruer)e) PH range 709--8936 ho(unregsa)tive contol exposure) 7983 solvent control exposure) Test temperature range (70.-9986.3ho(u15r)mg/Lexposure) 21.9 22.6C (negative control exposure) 22.1 - 22.9C (solvent control exposure) ~ Method ofcalculating mean m2e2.a2s-2u3r.1edC con(ce1n5trmagt/iLoensx:poasriutrhem)etic mean RESULTS Nominal concentrations: Blcontol,solvent contol, 20 mL. (bio), 20 mg/L (sbitic) Measured concentrations: <L0Q, <LOQ, 15, 13 mg/L Flementvalue: h24o-huourr LLCCSSO0 == 1>155 mmepl ((CCLL.. nnoott ccaallclualabbllee)) 7962--hhoouurr LLCC==SS>>001155 mmye ((CCLL..nnoottccaalleeubbllee)) Al clement values based on mean measured concentrations SEtlaetmisetnitcavlalEuveasluaantdicoonnfoifdMeonrctealiintteyr:val could no be calculated due 0 lack ofmoray. A`nAanlystyiscalofMettshtodsoolluotgiyo:ns were performed at Wildife erations! Ld. using high performance liquid chheromtaetxogrsaupbhsytanwcieth imnassthsepetcesotmestoiluctidoentse,ctitohne (HsPaLmCe/MaSn)d. Wmhosetn dpertoemmiinnenitng pteheakconrceesnptornastieonfoorf pCeormfplounoernotosc.taTnehseulLfoOnGate(mwiatsoufsedq.uanNtiotaatieomn)ptwawsas5.m00admeelt.o qiunanhtiisfystoundy.thTehbeasmiesaonfpienrdcievnitduraelcoovmeerryiocf meaetlisfaotrttiefsitcaitniiotnisatainoanlyhzaeddmceoanscuurrerdnivlayludeusrifnrgosmam7p8l.e taona8l2ys.i0s%waosf n9o9m.i2naSla.mpNleeswcsoalmlepclteesd cinolexlpaorsduraet Tours 24,45 and 72 had measured concentrations fom74.910 1.2 and 869 0.92, and 2. 0 8.1% of wm rR TEENIE [[msaeeae m JT aw: INTar te pe To oe _--,--_,--_ je hv-- 15 |WRN |NOAA | 0 T i ENVAMRDQ002)1 FINAL coxcLusions MARIA _ bmTsheeLrpvoettdhaeseslfiifmueicmttospefcfsooinlcuueobnritlorioattcyiaoinnewhualssfsn<ta1ut5desm.9T6Lh-heom0u6rg-LhoC(uS1rOniofsohrmosouhtereoapsy3fhecawodinmseiedmsrniaoiwsonwcawsoaodlte51to63errnmmiaenredid,0ibe 15 Submitter: 3, Environmental Laborstory, P.O. Box 3331, Pau, Misnesots, 55133 pommel 14 WI WL Reliab: Kimisch aking= REFERENCES_ - i his study was conducted at Wilde nermationl Lid. aston, MD at th requestof3, gree Last changed: 12402 et mw ENVMRD(2002)17FINAL 1R2o)bususltfoSntiucdayciRdep[oTrhitsNtuemtbwears 4p4.erfBoiorcmoendceusnitnrgatPieornfelsutoroofoScatlatne(Nsau,lfKo,niLcia)coifd,peproftlausosriouamlksyall((CTeosst substance number K-1520)] in carp TESTSUBSTANCE 1 `ISduepnptliiteyr::KK-i1s5h2i0d.a CPheermfilucoarlooCoc.t,anLeis.ulfonic acid, poassium salt. Lot ursber AS76268 R1e0m0a%rks: Test substance number K-1520. The test substanceis awhite powder. Purity determined tobe Meno ert bee --e---- MFiesthhoBdogduyi"dsetliipunleatfeodlilnowtehde:"MTeestthoMdetfhorodTefsotrinNgewtheChDeemgirceaeloSfuAbcsctuamncuel"atJiuolny o1f3,Ch1e97m4i,caRlevSiusbesdtaOnccteosbeirn 8A.ffa1i9r9s5.BuNroe.au5,, MiPnliasnntiyngofanHdealCtohoradnidnatWielofnarBeu:reaaun,d NEon.vi3r9o2n,menBtaaslicAgIennducsyt;rieNso.B6u1r5e,auP,harMmianciesuttriycaolf JIuntneern1a4t,io1n9a9l6T)"raidnethaendOEInCduDstGruyi,deJlaipnaens),foarnTd BeiocsoonftcCehnietmaitnciaolngs. Flow-through Fish test ( Guideline 305, GTyLpPe:(FVlIoN)w:-tYhersough system eSpaerc:iesO:ctCoabmepr 13,2000- February (Cyprinus carpio) 16, 2001 iSu.ppFliuekru:okFauk$u3c4k-a00k1e2n,yJaabpeang)awa fisherman's cooperative association (Address: 193-1 Yamauchi, Yame- Length Manedanweliegnghttha=t t6e.s4t-t9e.r6mination: Loading:Me2ananwdei2g0htug=/LNoreswpeeicgthitverleycorded FAniaslhyAtgicea:lYmeoanriltionrgiinsgh: High-performance liquid chromatography-Mass spectrometry Pretreatment were removed. ThTehefisfhiswherweercehecrekaerdedvisfuoarlly dinaytshe irnecaeifvlinogw atnhdortohuogshe dseymsotnesmtraftoilnlgowainngy aabnnoerxmtaelrinatly daicscilnifemcatiizoni.ng Aafqtuearriruema.rinAg,ftetrhethfeishsecwoenrde emxetderincaalteddisitnofeectliiomni,natteheyparwaesriteesacacnidmattirzaends.ferreTdhefo.fiasnh dfoermo1n5stdraaytsinign aanfyloawbutihorrmoaulgihtysydsutrienmgatthtiesmppeerriaotdurweesreofre25mo+v2edCa.ndThtheefriesmhawienrdeerotrfatnshfeerfiesdhtwoetreestrteaanrkesd aTnhde rfesahrewdearetshteasrvaemdeftoerm4perhaotuurrsebienftohreelsaomwpltihnrgo.ugh system for othe 27days. "NTuesmtbceorncoefntcroantcieonnt:ra(timoenasn:mTewasourpeldu)s:a Nneeggaattiivveeccoonnttrrooll, 2 and 20 ug/L DUepptuarkaetpieorniopde:ri5o8d:da3y7s.days Test cDoinlduittiioonnsw:ater: Groundwater fiom the premisesof Kurume Laboratory DiluStpieocnifwiactceorncdhuecmtiasntcrye:: Not recorded AHlakradlnienistsy:: 161.11gL PDiHs:so7l.v6e1d0O7x8ygen: 79 108.1 210 ENVIMRDQOD)I FINAL Stock andTetmepsesroaltuutiroen:p2r5ep0a1r0at2i5o.n5:CBascd on preliminary test ests for the96hour LCS0 value and wwaniaatslhystoeitnc-aal<sxdacethbelacantngikoendesltw.imiaLte,vttoteeplsrtee|cpwoanrarcsee1n2t06rauatgni/odLnsa1.no6dfmtLghe/evLeeslsto2cswukbss2toalauuntcliseoln.ws.erTehdeeceisdtesduabsstfaonlcleowwsa.sTdhiesscoolnvterdol tDeisltuwtaetrefrlwoewrreatseu:pp2lmieLdiminfostock solution and 1600 mL/min fordilution water; 2307 lesday for NExupmobseurreofVersespellisc:ate1s0:0 Ntorretank DNiuetm:bNeirpopfofnisFhorpmeurlvaesFseeel:d 4M0fg. Co, Lid. Waterchemistry ranges during the study: DIissolved Oxygen: | 8Ne0g-.5.Conmtroyl TemperaureC: 251-254C LT u80g-81o mg ouwl| 255 258C 250. 354C l" o 776-78 [76-78 76-78 LPihgohttopienrtieonsdi:tyA:rAirftiicfailcalligihgthotfowfhiwtheitfelufolrueosrceesnctelnatmlpamp(.14 hrs. day) e`Cxoplolseucrtei.onFooufrtiissshuewesrameptlaekse:n Aonuatlaytsicsacohfstaemstplfiinsgh twiamsepaenrdfodrimvieddedsiixntiomtswoagtrcouapcsh,laenvedlthinendubroatthiowneroef atahnneadletyxhzpeeendribimonetdhnitvwiadeluracelolmayp.nlaeltyAiznoenad.lyinsSdsiioxvifdfiuscahlolnwyt.erroBleetfciaaskuhesnweaotshuespaettrocfraoecrdhmseasdammpbplelefiontrgakttehinemeoeuxatpnefdrrdioimmveinadtneaedlfiiststhaorwttiahnsrgteea0n0gdrsoamufapleslr, fotergtuhmeenmte,ahseuardemveinstcoerfa pexicdepctonltievnet,, laivgerroaunpdofwremoainfiisnhgwamasteemrplwoeyreed.weTihgehtfeidshswepearreatseelyp.araTtehdeitneogupmaretnst; ocorngsainssteedxcoefptthaelismkeinntaerxycecpatnahle.ad, cals, fin, alimentary canal or ils. The viscera consisted of ternal aStpaptairsetnitcaslcmaedyt-hsoidast:e dSitveiaddeyd-sbtyatheeiomceonacnenwtaatteiocnonBceCnFtravtailoune.s caTlicusluaetecdofncoemntrtahetitoinssswuecroencceonntsriadteiroends 1a0t dbiefeartenatp.parent steady-sateif3 or mare consecutive sets of tissue concentrations were not significantly RESULTS $ ow MNoemainnamleacsonucreendtrcaotnicoennst:raNteigoantsi:v2eacnondt2o0l,ug. Bioconcentration factors (BCP): CPoFnOcS. Conc[eAnterratio|nAien rte?st water: [out bn[isi Ibns |bns (ug Tom Tie Tie A|bfwesraT lows lows ADaxes |oTD Tliosss [T17ieTiTsiss [20Ti0es CPoFneOS BCFsof Carp ExposedAteor22lan|d A2f0r 25ug/L: As wl{[o2s00 [a[Da0s L3|0u|0 TDoses | Dua | 7D2o0 m [up To T520 Ta Tee Tee [wo [ 3 Ir = iilAot So i [Ber Tao 000[1400 |Ti00 [3200 [2700 |2500 |i90|0 380 [32|0 ll Ei Dt ErmioT r----roCommemm] [=r rir |Concentration (ng/g) | 3490 |4750 | 5600 [4730 [9900 | 7410|9190 | 7650 18|11390 0 | (eer T2800]2400T2900T2400 5100J3800[4700[3000[930 [720 | CURLER bb ee Tp LL ENVAMRD(002)17FINAL DetAenrnmeinxi3n.g EffectApCpolnicceanttiroantoifonEsqufoirliPbFrOiuSmSPaalrttsiitnioSniilngaMnoddSeeldsitmoent h"Tihgehlriegvhiteedw othfethaebsceonmcpeletoef dseattaofdecsccortiobxiicnigtythteesttorxeipcoirttysopfrePseFnOteSd sianlssecttoiosned2.i2m.e1n,t2.a3n.1dsaonidld2.w3.e2llihnags toorxgiacintiysmosf PanFdOStesmaletssr,iaalndptlhanrte.i Sigennceeratlelsytsawciotrhrelaaqtuiaotnicbeitnwveeerntbtroaxtiecsityantodaqpluaanttisc ohragvaenidsemmsonlsitvrinagteidn ttohecownastiedrecrotlhuempnosasnibdiltiotxyiociftdyetroisveidnigmeefnftetancdonscoeinlt-rdawteilolnngfoorrgPaFniOsSms.in Tshoelreanids 5sedaicmeonntsetoqufiellnctaheendeaetda q`uaiplsibbarsieudmopnatdiatoanithnag malordeealdsy exist for aquatic organisms. One approach 10 deriving such data is 10 use Equilibrium partitioning models aeused in two ways: + cTooncecnatlrcautliaotne soilisediment pore water concentration from overall soiisediment Tocalculte PNEC,fisom eNEgCo. iIsn nbeoetdhedc.aseFso,rtmhaeneyqusiulbisbtrainucmesc,onKs,tsaonitlfsoerdidimsetnrtibiustiroenplbaectewdebeyn Kw.a.t,erwhaincdhsiosilesqeudiivmaelnetn,tK0soaislsusmeidinmgenttha,t aonnlaynitohneiocrsguarnfiacctcaontm,posnuecnhtassoPfFsOoSil,otrisevdeirmyenltikaelry tihmaptoritnatnertacitnioanffweicttihngththeineoqrugialniibcrisuumb.stHraotweewvielrl,alfsoor `bTeheirmepfoorretaKn,t v(aSlauleslofuoreatnaalp,pr2o0p0r0i)a,teassohlasorbseeednimdeenmtonwsoturlatdehdavine taorbeeceunsted.stuItdyshowiutlhd aPlsOoSbe(nEoltleedfstohna)t asadlsionriptytioafnfebcethsatvhieouSroloufbiPliFtOyS.of achieved (Ellefson), and as a FPuRrOthSe;rmtohree,hiigthisiounniccerctoanitnenotveorf winhtaetrsttiitimael-swcaatleer consequence the extent of adsorption may be ecqouuillidbrailusmo dependent wafofuecltd upon tbhee the concentrationofPFOS in the aqueous phase `Wchoimlpsatrtfmoerntallis ssuubbsjteacntcetso euxntcreartpaoilnattyi,onthoaft uPnNceErCtaifntryomisaqcuoamtpicoudnadteadtowhtehne ttheeresmiordiael oofr uspetdaikmeenits mdiefcfheraennitsfmoroofrguapntiaskmesopfressuernftacitnandtisffearreentcoemnpvlierxo.nmeTnhtearlefcoormepatrhtemeunstes.ofTehqeuimloibdreioufm tpoaxritictiaocntimonodaenldsthteo 01ain PNEC,u 05 PNECvimer 5 subject to considerable doubr, `nScohorommupalldlicmaatenityohnolidanoboefrxeattxrotarrpyaoplsoaoltiailtngioorfnrssoemdoitmheaenpsteplroeryasgualcntosirrstemoctPtieoNtnEtCrhestvualtltauskeesbsesncptopromoeparcaicvaoatuienltatbotlhetehetoshreegnacnotimhcpearmerattmitseenratscf.ounrtTtehhneetr ofofrthbeotmhattrhiex.tyFpoer osfubisntoarngcaensicthmatataidxs,ortbheprceofmerpeonstiitailloyn toofitnhoergaaqnuiceomuasttpehraistewandotbheeucnoenlcceesnstdarrayti1o0ncoorfretchte substance. This is unlikelytobe straightforward. pArospeeasrtcihsoofftshuebsotpaenncelsitweirtathurseimwialasrccahrermieidstoruytainndoprrdoeprerttoieasssteossPFtOhSe.enPvuibrloinsmheendtaplapfeartse aarnedrbeleehvaavnitoutro pthaepecrosnswiedreeratlioocnatoefd eaqnudiltihberiaubmstpraarcttsioanriengsuamsmaarriesleedvanbtelmowo.delI fiosrbPeFyOonSdenthveirsocnompeentaolf vestigate the papers in detail but it can be noted that the technical quality of the work, as tfhaies. with wSoervkera1l0 all open dJiotenraotturpe,rehclausdaelrteheadpyobsseiebnilpteyoefraepvpileywiendg(aelqtuhioluigbhrinuomt psaurbtjietcitontiongGLtoPPaFudOiS).buTthedofihnidgihnlgisgohfhtehesteecphanpiecrss! ifficules that mibeegncouhnterted. ns ENVAMRD(2002)1 FINAL Conclusion tItheiosrcyocnacnlnuodtedbethaaptpolincdthteobdaestiesromifnteheeitphreersecnotnlcyeanvtarialtaibolne dinatiantferosrtiPtiFalOSwattheartoefqusiillibarnidumsepdaritmietnitosnionfg PNECvalues for soil and sediments for the following reasons: thcoencennattruarteioonf; the adsorption process cannot be assumed to be linearly dependent upon tihnoergaadnsiocrpctoimopnoniesntli;kaelnydto be highly dependent upon soil composition, particularly the the rate at which equilibrium mibegachhietvedi unknown REFERENCES `Sgaelolloougimc, sMa.mJp,lesDuwdiatsh, vMa.rJy.i,ngMcmGiinlelr,alWo.gBic.alaannddMcuhrepmhiyc,alS.pMro.per(t2i0e0s0.).EnSvuirrfoanc.taTnotxiscoorlp.tiCohnetmo. so1i9l(1a0)n,d 2436-2042. SLoaiblorAadtsoorryptRieopno/rDtesNoorsptEi0o0n-S1t3ud11yofPotassium Perfluorooctanesulfonate (PFOS), Mark E. Elefson, 3M 2 ENVAM/RD(002)1 FINAL Review ofopen literature Report title Terrestrial isk Reviews investigationsoffactors | DeWolfe, | ChemospheJroeu,rnvaoll 36, no aalslsyelsbsemneznetnsesfuorlfloinnseatre ||tearfrfeescttriinagl efnavtiorfonLmAeSnt.inRtehfeersto |FWeaetzle,; Tom 6.pp 1319-1333, 199% a(LmAeSn)deidn ssouidlgse- cpraelcciipuitma/tmianggneeffseicutmofions in | Sadodribteidonftoormpriismnaortyesdoarpstbieoinn.g. | adivfafielraebnltetfootrhme(cfoomrmwehriccihaldlayta were. available). oErfgfeacntico-fcsaerdbiomneonntthe || sInevdeismteingtatoirognaonfitchceaerbfofnelcetvoefsls on ||CDayneor,S.MC.L; || Evonlvir1o5,n.mo.To8x,icpopl,14C1h1e-m. ttooxHiycuitlyelolfaaasztuercfaa.ctant || athzetescoaropftiaonniaonndictsouxrifiatcytatnotsH,yalella|ADelclarvalho, | 1417, 1996 | sSuplefcoinfaitcea)l.lySAtuBdSiesaliknydlicbaetnezdetnheat | 1h0ighhiegrhelrevaeplpsaorfeonrtgasonripcticoanrbon led ccooenfcfeinctireanttsi.oAn whaisghreerqusierdeidmoenctliit cthaerbtoonxilcevreelssp.onse at higher organic. tArnainosnpoirctscuhrafraaccttaenrtistics|| aIlnkvyelsteitghaetriosnuslfsahteoawneddathlatifaorran || ABlalrrreyd;, 4So1i5l4S2c5i., 1vo9l96161, 0.7, pp. inunsaturated soil | asulrkfyalcbteannzteninetseurlafcotniaotnes,wseorle. BGrloewnn,0. croenvderistiibolnesawnedrethqatuiecqkuliyliabchriieuvmed. as ENVIMRDQOO)I FINAL Amex. Summaryof the Lowest Acceptable Effect Concentrations EFardapsoien[ r Toh |s Pinpw haes roe ms [roNoomg | | emisewe | scion | (Fathead iow) FCrroimar || P(iFnaceshdaisrono)ms OECD02104K00OPFTS| 42.day NORCwpn Foarvc.emarrhneat |Dopehmeenre eonvyeGannact|| MOpsidosphsibr)i en Cmehricceo ||D(aWpahrmaPmeona Frewarn vcSheiinactnoes || (MMiiddoshbi)i i ohnai cnpitesiosn |||S(uhlownpPaercsscapkriciamunt Shotiem ESwraiitarhesiorane coSutrsem mtSiiimccnlatisines||| (SaanopanPsiersutsmobcboeptrmiecraineusm axons swFaenissntgohvitgher |Lemna iba Choibtioannisal || X(eAneohloweced fo) Sencionpmdent Seowraggaenvs em ASTM 8jt1hOFCD| 45how EC 7 OPPSROIS | hori 5 AS1T98M1 S98e1 rOtFaC | dy NOC 7 OPPS$501350 | Tomy NOE OBFOCSDWI00IE1S.AOESPPTTMS |||9SS6e8hh0rEEECCaCuamaainnr,nre | ia7) ShNOoE rcc | 44 TThhohow C EECaaprn,ns | 1uo2n0 72bout NORE ct OFFSS054 |[r9e6hsiECanel 5a11 |OFGACSODTO2M0T1EAIURSSEEZPA ||| 1M14a3d8y)NEEOCCRaaanncy E5iA orTssosm | TCs or e9Not6mihcEC,cotnrceeionnto | 7ii5y7 kin neh Oreo 216 J | aEr r | ae iORAL sT T ||hKehere -- c EmL Ea iLe e t Se |s ileer) 4e5l TnDEiLe| Pila, 3i1 ENVIMRDQO0)I FINAL Annex 5. Robust Summaries for Physical Chemical Properties and Environmental Fate Studies VAPOR PRESSURE "Title: Impinger Studiesof Volatility ofFC-95 and FC-143 "TEST SUBSTANCE I1d1e2n.ti2t,y3:.3P,e4r,fl4u,o5r,o5oc.t6a,n6e,s7ul.f7o,na8t,e8;,m8a-hyepatlasdobeceafrleuofroe-r, rp0oeatdasssPiRuOmSsaolr,FCCA-9S5.# (217-9O5c.t39a-n3e)sulfoni acid, 1, Remarks: The 3M production lot number was not noted. The test sample is a white powder of uncharacterized purity. METHOD - Method: Internal GLP: No `ear study performed: 1993 `Rmeemtahordkosl:ogicaDletcaoinlsceronustliwnietdh tihnistshteudry.esulItns asdedcittiioonn., thAesdodceusmcerinbtedsubbmeiltotwe,d tihsearecoamrbeinaatinounmboefrtwoof r`eapdodrittsi.on, tThheefifrsitstrerpeoprotrctoncotnatianinpeodrtPiaorntsoft1 wanodveIIr,sinonds.aAssecneointdherrepoortfth cofnrtstairneepdortExvpeerrsiiomnesntis#da3t.ed,Iint piSpumnkFnCo-w1n43whi(cPhFOsA)thewfaisnalpvreerpsaiorne.d iPnarwtatIePrroocredwuartee:riAsopmrioxptaunroelofso1l0utpiopnms FoCf-95th(ePFpOoSla)r-aorngdan1i0c Cfouomrpoocuhnedmsicallistceodnteinnttbhye LtCa-btlheerbmeolsopw.ray Amlaisqsuostpsectorfomceatcrhy bsootlhutbieofnorweearned aanfatlrybzuebdbliinngi2p8li0calitteersfoofr aiwrertehrkoeupgth itnheamn. icTehbeatfhlodwurriantgeobfubtbhelianigr.pasTshiengcotnhcreonturgahttih onsstboecfkorseolauntidonasftwerasbu|bbLliiminng. weTrheecsoomlputairoends aCofnttraaidnjiunsgtidnrgy ftoesrtvmoaltuemrieallosatnddutrhienng tbhurbobulginhg.glaPsasrwtoIolPartocreodoumret:empAeirratwuarsep0asasecdhatihnrooufgihmpainngapeprasr.atuAs 5a0n:y5v0olpartoiplainzeodk:PwFaOtSe.r soAllultiiomnpicnognetrasinwienrge5i0n0ipcepwmataemr,moEnxpieurmimaecenttat#e3wParsocuesdeudrien:thTehiemspianmgeersstutdoycuastcihn Part Il was conducted in duplicate at 90C. RESULTS `Vapor PressureValue: Pat Il 8.7 x 10 tor, Experiment #3-12x10"tore Temperature C: 90C in experiment #3 Decomposition: not sated us ENVAMRD(2002)17FINAL Part I Analysis before and after passing260 litersofair through various stock solutions, Results: Solution Original PFOS Conc., [1350000ppppmmTAemtmroanbuituymlaacmemtoartfeumydronide | 110000. ppm | 9% PFOS retained [og 7 g] 550030 ppppm m NL-aAulrkyyllpdyirmiedtinhiyulmbecnhzlyolr-ide |ammonium chloride 100 100 9 100 5br0o0mpidpemCetyltrimethy ammonium 00 9% c5h0l3orpipdem Tallowirmethy ammonium 100 5 c5h0l0orpipdemDicocodimethy ammonium 100 wa [Wa0Wat0teerp/r|p-PmProrApoamnpomaonnoilGu(m3O0:a5c0Oe)tatT e in water I~op 0100 |p90%] 500oppanpoml A(m50m:o50n)ium aceate in waterI~ 030 propanol (50:50) Tor 5p0r0oppanpoml A(5m0m:5o0)nium aceaie in wate1r- 40 % 5p0ro0ppanpoml A(m50m:5o0)nium acetate n wate1r- 050 5% 5r0o0ppanpoml A(5m0m:5o0)rium acetate in water 20 % uRDnre.lmiakEerdlkwysi:hnaTtTuhcetksheeesrefoinffdlitohnuegrsoCcahhpeepmmeiiacsratlr1syswDueegprgaeerstramteemnsomtvaaelld ltbohsyesUbonufibvPbeFlrOisSni.gtyaiHorfowtOehkvrleoaruhg,hoimnwacatoiemndrmiecdnauttesesdttaohtaetthdeitir1i2s/v7av9pe3or,ry dpwrreoerpsleseutcrseo.sn,cewnhtiracthioanreovferthyeloswu.rfacTtuacnktesritnhofuogahmt amnodreloiskselfyrommecthhaenbiusbmbslefdosloolsutifornosmatshefosaomtioofnmpihcaros:e Part I1- Analysisofimpinger ammonium acetate solutions. aRinemsypuilnttegsset:rsmaNitoserbtieealsltowmtarttahenersipdaoelrttewecadtsioffnroomumindtt,thobTseohlpeirdcesaslteconutlaaiitneadenimtdhaetxrhitemheuimfnitropstrteohsreesuareemcomwnoadnsii8mu.p1minxagc1ee.0tatTteohries.soliuntdiiocnasteisn tthhaet seRrhetomouanlrcdokuusss:elyctahIlenlvetahdleu"remeip0on.ri6tm2,m5:utgnh,ne"nmovtaaxp0oi.r6m2pu5rm:egs/svmuaLrp.eorinIptnrheasdsdtiuetrxiteobnce,alltcohuwela"btomitoahnxsciqsmueaucttmii"oonnvsacpoonartnadpir7nesserurroersc.alcEuqluataetdiownas.| cpDrro.ensdsiEutdriweoinensquTdiuolcinkboertrioupfmroatvhisedeCahtfeaimiimnsetdervbyiedDteewnpecaeernttthmhaetnttshoealtidntuahmenbdUentrihveiesrfslrioetwayisoonngfaObglakesl.ahTohmearesaits ensotheavtitdheenceexptehraitmevnatpaolr 29 ENVAM/RD(2002)17FINAL Experiment # 3- Measuring Vapor Pressure at 90C mReismnuiltmusu:mmvTahpbeoercrkpeurpseoerstsutcrhoeenfcoirltmhupdiensgetetrshtattmraaPtienFsrOimaSlaywhaassnopaturmhpeaoavrsetuercdaabtuolgbehetvaa1pl.2orofp1rt0eh"sesoufrrleeuoaratto9c0hheiCns.mitceaItmlpwetarhsaattucrohena.sdidbeTerheeedn Volatlized. oE Resma0h r.k6s3:5ofuTg,thheerTeahnaaelryectoircneacalesnomtnersaasttiuoornecmorneasnnitgdseerifntrthohsmi4istmqupudieyns,tgbieuotntarhbaelienqur|aesniutlnitf.EixcpaNteioroinmneoitnmattii#onnt3hwwaeassanma0al0yd7sei-s0ai0bnP7oa5urtythg1e.1 bVeilonwetrhecqounacnetinftircaattiioonnsirmaintgreedpofrrtoemd i0n-0P.r1t8 Iu.g in impinger tran 2. These concentrations re nearof CONCLUSIONS DNaouvreerlsyblleowcovnolcaltuisliitoynsoracanvebreymlaodwevabpaosrepdreosnsutrhes usntuddeyr. amTbhieengtenceorndailtioobnsse.rvation is that this compound Remarks: none, DATA QUALITY. SeRebmlsimtaaibnincfeiit.niegsT:hveolraKetiiilmsiitnsyocoihfnftroharnmkatitenisgtons3ou.bnsttTahnheceevr.aelidiTishtyinsooftsihtnuefdoyarnmalaaltcyiksosinscmoheantrahtcohtdee.rivzaalitdiiotnyooffthteheputreisttymoefthtohde tfeotr REFERENCE. 3M Environmental Laboratory. 1993. 3M Lab Request Nunber 3306, 3M Company, St. Paul, MN. m ENVIMRDQ00) FINAL WATER SOLUBILITY STUDY "Tiles Solubility of PFOS in water T TEST SUBSTANCE e 1Id:e2n:t2it.y3:,3P,e4r,f4lu,o5r.o5o,ct6a,n6e.s7ul,f7o,ra8t,e8;,mSa-hyepatlasdoebcaeflrueofreor.e,dp0otaasssPiRuOmSsalotr, FCCA-9S5.# 2(719.5O-c3t0a0n3e)sulfoic acid, 1, bFReCe9m9a57r..k9as%n:dbyTaLshieOgMtneSestd,s3u"btHess-ttN,anMccRoen,tios"laF,-wahNniMdtRerepafonewdrdeeenrlc.eemneTnuthmaeblesaranmaTlpCylseRisw0at0es0c1hrn7ei0cqr4uy6ess.t,allTihzeedpruroimtyawaprsodduectteiromninleod of METHOD ell NN - Method:Basedon OECD 105, OPPTS 830.7840. GLP: Yes Year study performed: 2001 eRaxnecdmeassrshkaaskme:onuanWttao2t2fe5rtresoptumrscuaetbs3its0anAcCSfeoTwrMit2h4T,ytph4ee,aIpopwrrao7tpe2rri,haotMueirlwslaiftpoeolrrleo.inwecTdehnbetyrdi2eff4ui-gnehiottuiurvbesesto.efstTeqhcuoeinlsitibusrbtaeetsdiownoefr1tpl2va4co.icn3g5aa.ln FTioqluliodwcihnrgoemqautioligbrraapthioyn,itshammpalesss swpeercetrcoemnettrriifcugdeedteacntdiotnhe(LsCuMpSer)n.atant was analysedby high performance -e RESU. LTS Value (mg/L) at temperature C: 680 mg/L at 2425C Descriptionofsolubility: Slightly soluble PH value and concentrationat temperature C: not sated PKa value at 25C: not stated sRoelmubairlkitsy:coTncheent2r4a-t,io4n8s-., and 72-hour solubility concenwetreravearatgeidtooobntasin the overall mean CONCLUSIONS "The overall mean solubility concentrationofthe test substance in pure water was 630 mg. Remarks: none A DATA QUALITY eet Reliabilities; Klimisch ranking | ill LIE om ENVAMRD(002)17FINAL foREFERENCE NElulmefbieorn,EM0.0-12701061.c. SolubilityofPFOS in Water. 3M Company, 3M Environmental Laboratory, Project =m ENVIMRDQO)I FINAL WATER SOLUBILITY STUDY ile: Soubily of FOS in Natural Seawater an an Aqueous Solution of3.5% Scum Ciride TN EST SUBSTANCE e e1n2t.i2t:y3:3,P4e,l4o.r5o,o5s,a6n,e6s.u7l,f7o,n5a,t5;,mSahyepsstoabdeecraelfecrreodp0oastPaRsOSsaohr CFCA-9S5.# 3(19-O3ct0a0nsesulfoni acid, 1. RFCe.m9a5r,kasn:d aTshseigfnsetdsautbesstt,anccoentisola,wahnidcepordee. uTmhbeesramTpCleRw0a0s01ec7y0s0t6aliTzoetdofoym sperodpuctmionotof be 97.9% by LC/MS, "H-NMR, ""F-NMR and elemental analysistechniques. b> SH LRA Method: Based on OECD 105, OPPTS 830.7540 GLP: Yes Year study performed: 2001 iRwfhoeaelmtlaesorpwiekedrscm:ebanyttTar2hni4etfuhdgoveeuatrfsusibeoansfn.iacleTqyszlhteldcbtouanbbisyeiossnhtwieegadrthoef2ppv-eol2rrfe4con.neindaannaFcnoedlxlcsolehiwaqsiuksniegdnameSouwui1onb5mtr0omapoftgomteensi,1tp3sshu0ymbsptCwlafinsochea3wm4is.tsh1chmeocpmoormsoneppraesres decom (LMS) Ra esuLTyS tl Water Sources: Natural Seawater = Ocean Scientific, lot # LN 58, salinity = 3.5% `SodiumChloride = EM Science, 99% pure, mixed with ASTM Type 1 water to achieve salinity of 3.5% mVayluae (2mg2/0L)Cat temperature C: NaturalSeawater: 12.4 mg/Lat 22:3.2 5%N3aC lSoC luti; on: 20.0 Description of solubility: Sighly soluble PH value and concentration at temperature C:notsed pKa value at 25C: not sted lssRooelllmuufbabiirltlikhttesyy:roecfpoTlntihhcceeaetr2seioa4rdniiao4uynms,secshfalonfrodroti7hdte2eh-tnchalodtcuaururyaasltoilwsuoeabnaiswlbia1tetyce5arc%,uos.nccTwheephte3i4o-tnhhesowucreoorviacliuaeecsnrtawgoiefsdi(or0tboacniondwaetshdecoeveuriaalelcsmoeoaern coNcLusions teeta (lL 5T.h5e%onNeaCall smoelautniosn lit uwats 2c0on0cemnetrla.tionPRofOtSheSatlesbtilstubysdtaenccreeaisnenawtiutranscaetveisnwbaise12e4emln.ofInhe m ENVAMRD(2002)17FINAL Remarks: none DDAaTA QUALITY-- Reliabliies: Klimisch ranking | Remarks: none REFERENCE CFhllloerfisdoen., M3.M2C0o01map.anyS,olu3bMiliEtnyvoirfoPnFmeOnStailnLNaabtourraatlorSye,awLaatberPraojnedctanNuAmqbueerou0s0-S1ol7u1t6i.on of3.5% Sodium 2 ENVAMRD(2002)17FINAL SOLUBILITY STUDY Title: Solubility ofPFOS in Octanol TTeEST SUBSTAeNCeE I1d:e2n:ti2t.y3:,3P,e4r,f4l,u5r,oo5c,t6a,n6e.s7u,lf7o,n3a,t8;,m&a-hyepatlasdoecbaelrleuofreor-e,dp0otaasssPiFuOmSsalotr, FCCA-9S5.# 2(719.5O.c3t9a-n3c)sulfonic acd, 1, bFReCe-m99a75r.,k9as%n:dbyaTsLhseOiMgtneSset,ds1u"btHes-sttNa,nMccRoen,tios"lFa,-wahNniMdtRerepafonewdrdeeenrlc.eemneTnuhtmaeblesaranmTaplClyseRisw0a0tse0c1rh7er-ci0rq4yu6se,tsallTihzeedpfurrotmyasapsrodduectteiornmilonf soof tz AAI A -_ Method: Based on OECD 105, OPPTS $30.7840 GLP: Yes ear study performed: 2001 SRcermeaernkTses:t:OcLtoatnoJlUOSoBu7r3c8e0:4,Al9d9r+i9c%h TTDhhefeeitndueifbtievnseitwTieevsrete: tseLhtoatckoe0nn6sai2ts3t~8e1CdI5,o0f9r9ppl.ma9c4iat%ng3H~0P0L.C0C10fGorrga2dtee4st ,su4obs8t7a,2ncheowuirtshfo~l1l0owmeLdboyct2a4nohouirscoefnterqifuuigjebrtuabieos.n ap8er2f2o2r3maCn.ce lFioqluliodwcihnrgoemqautioligbrraatpihoyn,wistahmmplaessswseprecetcreonmeitrrfiucgdeedtcatnionth(eLsCupSe)r.natant was analysed by high a RESULTS -- Value (mg/L) at temperature C: 824 hhoouurrss:: 3$56.97 mmgg/lLL. 72 hours: 55.4 mglL Mean solubility ofPFOS in octanol = 56.0mg/L Description of solubility: not sated PH value and concentration at temperature C: not sated Ka vaalt2u5eC:notstated Rsoelmubairlkitsy:con`cTehnetr2a4t,i4o8n, and 72-hour solubility concentrations were averaged to obtain the overall mean 2s ENVIMRD(2002)17FINAL BoCONCLUSIONS "The overall mean solubility concentrationof the fest substance in pure octanol was 36.0 mg/L. bwSieutcbhamuisstoetlueobrifslitthyReesdmciraferfeikncsiu:nlgy rTieynspuiolcbtatslaoliyfn,i<n1tg0hetmuCbLoinl.gucmoHmoEpwlaeutvtiieborin,eMtwehitethhsohodacktiesanfrolelacskaonmmdmeettnhhdoedepdowsafssoirbwluesieenxwpdiltohisnisoutnbsshtasasnoacdres's pos by possible leaksofaflammable solvent in an incubator. DATA QUALITY Reliabilitis: Klimisch ranking | Remarks: none BeREEFrEREtNCEerorrrecassesmetetasemtteemeermemesemetmeteseoeenmetmta LElalbeofriaotno,ryM,Proj2e0c0t16N.umbSeolrubEiOl0i-t1y71o6f.PFOS in Octanol. 3M Company, 3M Environmental Laboratory, 2 ENVAMRD(2002)17FINAL SOIL ADSORPTION "Tiles PilotStudyon Soil Adsorption ITEeST SeUBSrTANa CE , I1d1e.n2t.i2t.y3:3,P4e:r4f5l.u5o6r6o.o1c.t7a.n8e.s8ulSf-ohneaptteamdeacayfallusoroob-e, proetfaesrseidumtosalals, CPARSOS#o2r79F5C--9359..)(1-Octanesulfonicacid, RToetmnaurmkbse:r wTahsensoatmnpolteed.waTshreadtieotlasbueblsetdanicseotwoapseaawndhiltabpelingopoosfwiutnocndhanroatecstpeercriifzieedd)p.rTyh.e 3M production METHOD re CwMhheirtlohemoaldtgougoirdaeopflhoiyrknaePlltiaetfreonnslafltoiogvwreedsde:tfeorTmshtieundiynisgntgutdthyeheseaoxidlpslomororpbetidiloitntydheeosfaruPpsFciOoSn.opfrTohpaieusrttoimreeastdohifoogdhreawpathesysdseouvfbesltToahpniecned. bLyaysevr GLP (VIN: No Year study performed: 1975 Statistical methods: None Temperature: Notindicated Remarks: Lite detail was available regarding the study method. RESULTS bReesvuislutasl:izTedh.e study used TLC greenhouse sol plates. The radiolabeled spot due to PFOS was (oo fant 10 coNcLUsIoNs eee No conclusion could be reached in thisstudy. lH] AAY LLSS -- sRfpoeerlciirafabidiciloiatscyyt:niivhieKtsyiinasinwsdaccshhearmlaisncokainlnogrta3pd.riooTvchihedesemedi.csaTtluhdepiuerasintalylaycotkifecdatlhsumftefetischiosednuotblsdoteganyaclelarwceekgeraderdvniaonltgidpamrteoitvohinoddeaodnl.dogTaya.smemIasenottsohpoeo.f identifying and quantifying potential degradation products. R REFERR ENCES BMoiycdh.iSg.aAn.Sta1t99e3.UniRverseityvo.fiTechenicwal Notebook. Soil ThinLayer Chromatography. Number45277, p30. `MTeencdhenli.calAN.ote1b97o6o.k.SOoicltobTehri1n3,La1y9e7r8.ChNruommbaetrog4r52a7p7h,yp--3F0C..9Pr5o,jecFtCN-u14m3b,erF9M9-73402621.260E0x,cerpt from 3M wm ENVIMRDQOZ)I FINAL BIODEGRADATION STUDY Title: DecrminationofMethylene Blu Activ Subsiance-FC95 TEST SUBSTANCE Len1t2i2t3y3:44Pe.s5f5l6o6r.o7o.c.u8n.es5u,f5onetpstamdacyaalluorbes-repfaesresdio3n3aP,ROCSAoSr4F2C7-9955.3(013-)Ocanesalfonie acd, pRuemsa:rks: The 3M production ot was 158. Th est substance wawhsic powderofuncharacterized eA Methodiguideine followed: EPA Method25. Test Type: Methylene Blue Active Subsance GLP (VN: No Yearstudyperformed: 1959 Contact tim unis: Not indicated Inoculum: Not indicated ResuuTs Degradation 4 ste ime: Not ndicsed Ress: [3 LbReGqiuseisot1Namber | MBAS Result Kinetic (or samp, postive and negative contol: Not indicted Breakdown products (eso): No indicated Remarks: None concuusions Reviewer's remarks: The st indicated tha th est sample exhibited igh activity inthis MBAS say. DATA QUALITY Reliability: Klimisch ranking 3. This study lcked information regarding methodology and esing nm ENVAIMRD(2002)17FINAL dtpehasercaFrmiCep-tte9ir5osn.wafTsreossmtenTstwuibinsnt2aCsnioctleyutpTiueorsnttaiynngdwitwthahaistnth"tlhiqeaucittdeus"at,l sbMuatBmpAFlCSe-9wm5aasywanbsoetahissugfhofelirdcitemhnaattnleytrhicahalvraaTlchutieesrsimezeaedy.d iTnhdiecastaemspolre REFERENCE eee ee DMientneerampionlaitsi,onMoifnnMeestohytlae--naet and 1828, BtlhueereAqcuteisvteoSfutbhseta3nMce-CoFmC-p9a5n.y,19839M. PLaacbe RAenqauleytsitcanlumabnedrsT:wiGn15C1i3ty, T6e1st8i2n5g.. 2 ENVAMRD(2002)17FINAL SOIL ADSORPTION "Tite: Soil Adsorpion/Desorption StudyofPotassium Perfluorooctane Sulfonste (PROS) eaTEST SUBSTANCE 1e:n1t2i2t.y3:34,P4er.f5l5u,o6r,oo6c1t.a7n8es.u8l-fhoenpatteasdemcaayfallusoorbo-eproetfaesrseidumtosalas, CPARSOS#o3r79F5C.-3995..3)(1-Octanesulfonicacid, tFReCec.mh9na5i.rqkuesTs:h.eTphueritteystwsausbsdteatnecremwinaedsa twohbiete9p7o.w9d%erb.yTLhCe/sMaSm,ple'Hw.aNsMrRe,cry"sFta-lNliMzRed,farnodm ealpermoednutcatlioannaolytsotsf METHOD iL I Method guideline followed: OECD 106 a GLP (VIN): Yes Year study performed: 2001 S1t0a6tiusstiicnaglMimcertohsoodfst:ExcStealtistical analysis and plotting of the data was done according to OECD Method `Temperature; Roomtemperature (19-30C) spSortleoulctikimoinananrdoyfeesxutpnerrsaiodmlieulntati.boenlFepodrr_eppetarhrefaltduieoofrinon:oicttivaTenheeesxutpleefsrtoinmcaeotnnetc,entteorsattaisoonflsiuntaailnodnsfecsowtnedriestuibomsnatsdaenwceebryecddoienltcueetrnitmntiginoeand.stinocokf approximately 0.5 mL in aqueous 0.01 M CaCl 20 ssruniy i [wow|woes|wis|mor | om |] Sore || aNorhmanod|roraio,n.| LNoarbtoorn,,| aNbornmoarn.,||LNoorrnaiso||DoJneT. Deeripton | 160,06 | ic0.06" | died06"| ied,0.0 |drew 04"| cverned, id deep deep deep deep 1 deep sterilized %Sand |1 " 6%| %Sil[ t 22%Tse| ae% 58% |a | 39% | awe |Notanalyzed |Notanalyzed [2% Clay 62% 11% 20% | 19% |Notanalyzed| Btntro cr,te ope cy ste rE BE ENVAMRDIDITFINAL ResuLTs LL ag Adsorption Kinetics of PEOS, 1:5Sol:Souton Rat, 48hou Tim Poin i[l SIE Ieeragne DisDtirsbitbruitbyiuction| OrPeemrciecnCtaargbeoonfin|Chohon nngnoeorDrmmaelagilassed Clay 0.0183 Coecient, Ko. 26 Jes=" SCeldaye Llooaamm | 0g.u00i9s7e2 1 p2s6 p IS--T --] | RiverSediment A Domestic Sludge |<0020 "7T fs--fr Notavailsble | Notcalculable | HaAnldomPaoRtfuOieSxcprswoasasusdrsneoorabdeneddtettchhteeeetdessittnsstuuhbbessettxaatnnrccaeectsc.eoonTnchgeeln.adtaTtiahoneingddiidcaemtoetddvteahrmtyosSngsonsiohtcdanmvelcryyohsmsoensg1 wdhsomenpivoeno(e-96%) Apparent DesorptSiaoin KTiynpeticsofPROS, 1:5SDeosolrSpotilounoCnoefRaftiicoi,en4t5,-KhoourLTsime Point [CC ClayLoam | DFoimveesrtSCileacdySihmuent 0.000158 0"000001020307 Q0pTuehrieicokddsl.yet;TrashfuetaebistvdtheeeartnScschwtiobalsumep:iaommdloipyupnoldtiaenysoeotfdrebteetehdtestfsmrusobosusmtbttaihdnaeceness.coosrlDpceioesondoncraiepmniet3oann9o%ltnofdaigdredio4mdta5ttvhieacwguessSoduTeorihnpngmetashsenl4e5m-nheomyurccso y Adsorption Isotherm 1 SoilType: Log K*y [Ca~ vClLaoyam] 1152750125 R|iSvaenDdroymSeesLdtioimcaemnt| 20261 l= 1.246 (Oundle adsorpionsoefiien = Regression constant, Un 170G0o5t0|aossste | 000095194 00598209 0.0368 1.2581 Regression Constant, n IL1h1o3 1T2o1 0.795 m ENVIMRD(2002)17FINAL [some | worn [wmv| Krom Em Desorption Isotherms oT ShaClyalyoa[ m |018 084 TTTT" am "ool DRoimSvaeensrdtySiecLdSio[ muaemdntos"1s .d4l7 TT oq00039 205 (1)Fecundich desorption coefTicient Regression Regression 1os | 0986 1 aor] | 10s] | t10o2r | o09o84m] 0327 306 tiFnhrdeeiuacnamdtoleuitcnhhtataprdtehsesoerdnpatttiaionnotbihaesioantqehuederomosurswtseholrutteusitsonesduatbtocsqtrueaillnaitboerituhmve.(aTwmhoeooeurndvrtearlousefsotfmecsaatlgcsnuuilbatstuteaddnefcoeweatrdheseosrrlebiggerhdetslosyninoonthnsefonsniealatrtao BCoONCLUSIONS (m1at2r1i1c0e0s)Pteaersstfetudh.aeroKPayFcOivSanlcusweuosluaflrodena>not|t (mbPegFOc.Son)Tshiadepeprteeeasdtretsdoubtbsoetaaqnducasel,oirtobantcsievieoalnydgslmoyrobbteiodl,ealdaoseospfetnrhoetOsEdieClssDordbiGumeiedanetllis,nieuvdoegene hianpepernsosxeixtmtharataneclt2ey4d |whiomtuihrnsa,untweoirtoghfansciuobcnststaoacltnv.teinatTl.haeIdnstcoeristptthsiruobncsa(tsa>,n5ca0e%)edxohsicbciuotreiodrrndglpeosiwonirsmpoootbimioenlni,otyfantihneeqaultililnioebfr0ihusemamapiliseoscsbaeofndsesr vtiiensatteertdah.cetiTmohen.echsBahenacipaseumosoeffPtcFhheeOmSPisFoiOrspSatisaotdnrs.oonrgptaicoind,isitotlhikeerlmy (fFoir-myspes)trionndgicbaotneddsawvietrhysoSitlr,onsglucdhgeem,icaanld/ssdesdiomrebnetm DATA QUALITY a Reliability: Kimisch ranking | REFERENCES ee hi E3lMlfTseocnh,niMcLaEl. Re2p0o0r1t4.. PSrooijlecAtdNsuomrpbteironE/0D0e-s1o3r1p1i,onCoSmtpuldeytoifonPdoutaessfuinucm4,Pe2f0l0u1rooctanesufonstc (PFOS) 5 ENVAMRD00)I FINAL BIOCONCENTRATION (PARTITION COEFFICIENT) c-"aiOctchlto:afntoKhloewwAiqt(SuhoelSouuubbisslieMtqayuteriinncteWsaC)taelrc,ulNaattiuornoalfStehaewant-eOrc,taAnnolAqWuaeteorusPaSrotliuttioinonCoofef3i.c5ie%ntSo(dKiouwm)CholfoPriRdOe,S and TEST SUBaSTCANeCE I1d1e.n2t.i2t.y3:3.P4er4f.l5u.o5r6o6o.c1t.an7e.s8u.l8fo-nhaetpet;admeacyalaulosroo-b,eporteafserseidumtsoalats, PCFAOSS#2o7r95F-C-3995..5)(1-Octanesulfonic acid, bFRyCe.mL9aC5r,/kMasSn:,d gT"shHse-iNgtneMseRtds,uthb"esFti-anNntMceemRwa,laasrnefdaeerwlehenimcteeennptoawldaeunra.loyTm sfihTseCRtsbe-ac0hm0np0ileq1eu7e-ws0,ar4s6r.ecrPyusrtiatlyliwzaesd dfertoemramipnreoddu0ctbieon0l7ot05o,f METHOD OMePtThoSdi8g3u0i.d7e5i5n0eanfdolOlEowCeDd:1_07C.alculated from n-octanol solubility and water solubility according 10 "Test Type: n-OctanolWater partition coeficient (at saturation) GLP (VIN): Yes `ear study performed: 2001 Test temperature: nW-aOtcetransoollubsiolliutbyilviatlyuveaaltue24at-2253-C24C dtRihedemsanhroakkseb:rfilnTagshktehmepehttyhswioocdaplpheaprsreogspueir(dtnai-neocscetoapfnroPloFviaOdnSedddwiiadntetnrht)e OainlPtlooPwcSoantadanectdteOrwmiEitnChaDtPioFgunOiSdoefltaithneetsh.peaTrshtaeimroeenfotcrioeme,ef.tichaisnednstttuhbdeyyy Wptaeatsertrtiniagonnrdefceloeexcfptfreiedcsisetinhntegwpiatarstsitctiaholenculclooagetafefrdiictbiheymnitcdifvvoairldueti,hneg stuhbejescotlubmialtietryiaolfaPt EsaOtSuraitnionnocotnlayn.olThbey nh-eOctsaanlobliWlatyteirn cAAEA AANA eo mThLe cailncuwlaatteerd).log Kow for PFOS was determined to be 1.08 at saurston (1og(56 mg/L inn-octanol650 CONCLUSIONS trent `Rwehmicahrkwso:uldNonoctonTckleulsyieoxnisstcinothbeueednevlriirvodendmerntom this information. I applicd onlyto astrated system, DATAAQUAY LITYee tee | LL Reliability: Klinisch rankin|g Pe ENVIMRD(002)1 7FINAL sRVyiemsmitteaemrds.k.sA:pplTihceastoolnuboifltithyisstvuadileus iweareisckonadsuscetsesdmepnrtophearsy.liHmiotweedvoerr,otvhaalppulaie5caibiolniltyyopflihiessd0ataspuominmteids H REFERENCES y oKOfcottwahneo(lSAoqwluiutbeiholuiStsuybMsiaentqruiWecaentste)r.C,al2Nc0au0tl1ua.rtai3lonMSoefCatovhmeetpeanrn-,yO.cAtnaSnto.AlqPuWaeauot,uesrMiPSnaonrletuisttoiitooann.CooEefnfvf3ii.cr5io%ennmteSno(tdailKumLooaCbfhowiProaRrt)OiodSrey,fParrnsdoajnee. Number E00-1716, 235 ENVAMRD(002)17FINAL BIODEGRADATION STUDY SToiille:BioMdiecgrroabdiaatliMonetabolism (Biodegradation)studiesofPerfluorooctane Sulfonate (PEOS) IL. Aerobic ITEST SUBSATANNCCE hIedpetnatdietcya:fPleurofrloo-r,oopcottaansessiuulmfsoanlatt)eCA(1S-O#c2ta7n9es5u3l9fo3nic acid, 1,122,33,4.45.5,66.77.8,88- Remarks: White powder, 86.9% purity, alsocalledPFOS or FC.95 METHOD _ LMaebtohroatdogruiiesd,elIni.ne followed (experimentalcalculated): Method wasdesigned by Springborn "Test Type (aerobic/anaerobic): Aerobic GLP (VIN: No. ear study performed: 2000 Contact me/units: 20weeks bfIoenrloeocstwultiunhmeO:nWsaeTrthc,ehMaimAn,,ocMaunAldumwaasrsoituverewrcaetbsearnwketrrieenatsBomrieilndstgecpowllaaltneetcrto,eutdMfaAfli;loamanandfhrasoermddiwtmoheeondNtsafrocrroeolswltescitrneedHaafnirsnoomWna,brrMeachAka,ims,ha Mpeisnse aBnidomwaasssrweapsordetteaedrm1i7n.e4dmoenCd/a1y080g3 sboyilbaontdh 6txhe 1f0umcieglaltsi'o,n/reesxpteaccttiiveolny.and standard plate count methods mwRieenimegarhratlkspoo:lrt_uitonSisoo.inla,aAynedanssutetrdeiixetmnretanctmt,isxaatnmudprlereeswagawesnetrperwaeatpeairr.ieedTdbh,ye 2sco.oi0msbmeimdnsiiimneegvneatdms,tiexartniluderempoiwtxatesidnagdtjosuogsielttehedexr1t0rian7cc5tq%u5aolfftarhcyee msatgtu/dekygr..hnoTdlhdieandigjnuccsautpebadactiuitsoyinnutgserimenpagegretahnteturwaeabtowevaresans2u2tnre1ieed3ne8tdC.m,ixaTtnuhrdee.tnhoemSitoenislatlwmaoteisssttccuoornnedcuewcnattseradmtoiinnointthwoeardesadrakpw.pereIoknxlfiyomradmtuaertliiyonn31to-hn3e OlteimsnpteOdsNoslEeuNptittousnmaatsgicttreaesttwioicnnaiwtpiasastcioonnno.ttapiSrnaoivmnipgdle1ed0s. gwTe(erdsertytvweaskeseienglsha)twedsoraieylss4e70d,imm1.4e,n12t.1C,mhi2xe8tm,urg3el5.a,ss4E2va.icah4l9s,twei5tt6h,lasainkldicro6en3ce.eTievEfelndotnae. `nsTyahlmeopnlstefosiclkwleesrroeplruietoxirtorntaocutsaenedadltwyoisitdsho. smeeStahtamhnepolbleisovdiwaegearrcacdadltieliruottneedtdeasstsolsnvyeescntetesmesxsatrrwyaacistniopmnre.etphaEarxnteordlacattasndpcaoasnnecaedlnyttzrehadotuiwognnh oaLfC0.1V2,I06Sm0n dmeet/eLn,miwnheiscahniisngapcporncoexnitmraatteiolnys.twice the water solubility of PFOS. No. day 0 samples were saken 10 e Degraday tion % after time: Not specified llLE L tssesame| Results: Essentially no PFOS metabolism occurred duringth study. 236 ENVIMRD(2002)17FINAL Kinetic (for sample, positive and negative controls: Not specified Breakdown products (yes/no): Nore indicated Remarks: None oCOeNeCLeUSmIeONsS Remarks: PFOS is recalcitrant inthe activated sol/sediment sys, DATA QUALITY n ssRtaeolmcipkalbeissloilwtuyet:iroenKtluaiksmeenidstctoohddreoatsneekrimtnihgnee=ssyt2.satreiSmntsgucdwoyanscneonpttrrecaptoainrodneusdc.taetdtwaiccceortdhiengwattoerGosooldubiLlaibtoyraotfoPryHOPSr.actiNceos.DaTyhe fRsEFERENCE T3hMeCsotmupdaynwya.s cRoenpdourcttceodmaptleStperdin1g0b/o3r1n/00L,aboLraabtoPrriosj,ectInnc.umWbaerreEhOa1m-,04M3a4s,sachusetts, at the request of the w ENVAMRD(2002)17FINAL BIODEGRADATION STUDY STiltuldegseMBiicordoebgiraaldaMteitoanbolism (Biodegradation) studies of Perluorooctane Sulfonate (PFOS) 1 Anaerobic I TEST SUBe STANCE hIdeepnttaidteyc:afPieurofrlou-o,rpoootcatsanscisuumlfsoanla)tCe A(1S-O#c2t7a9n5e-su3l9f-o3nic acd, 1,122,3,3,4.45.5.661.78.85 Remarks: White powder, 869% puity, alsocalled PFOS or FC-95 ee Ll Ul - LMaebtohroatdogruiiesd,elIni.ne followed (experimentalcalculated): Method ws designed by Springborn Test Type (aerobic/anaerobie): Anacrobic GLP (VIN: No ear study performed: 2000 Contact Gmelunits: 56days pIlnaonc.ulum: The inoculum source was an anaerobic digestor at the Rockland, MD wastewater treatment mtRreeedmaiatumrmek.nst,:BTrhiAdegmemiweaxttdeuirra,e wMceAorn)te,aiupnnrisenppgeacrdierfdiieeddunsOdleEurdCgDneitmerixontgreearncatlpm(uefrdrgioeam,1r0aonteadtxircnelgsuadbzeiuorloioxgyiigncedanil.cactoonrtTahsceetrevrmedowmaamssathe)ewat1teenr ticeoesntrcoseonflturmtaeitdoiinounamg.witaastTiahopenpwreoasxtsitmneaomttpepelrryoavt2iu0dr.ee8d.wmayTsw,3enSatnyCd,|l6ao0nadd-imtnLhgesitneersetusmtwabvsoetstcsloeelnssdcuwocantstead3in0iin0ntgmhle1.0d0aarnkma.eLrooIbfnifctoerStmuastgeieotnipoaonnr 3r(5ep.cuer4ig2ve.edd4w9ali,ltahcnodnmipt5or6no,egeannntdsaafferroemsfiilnnlioinctgu)ilowunim.thcocnStrarimomplplebedsorwbeuetsryelontraukdbeabnyesrfr7toomapnstdhse3e6tr.evsetdFboaor5tatneaoslnysfidlsaa,sykssa.n7,aBl1ai4c,qhu2,o1t.fw3ak8n. caronenamcloeyvnzteerddatvfiiroanomLoOfeMa1Sc,h,06b0oTtmthgle/eLsa,tnoadcpkpcrseonotxlruiitmfiauotgneedlu.ysetdwBitoochdtohtehsewtashteueprbeismooadltueabgnirltaidtaayntoidofntPhtReeOsSsto.slyidsNtobemidsoamwyaas0ss spparometppilaoernsedwwaetree5. taken to determine starting concentrations. RESULTS Degradation % after time: Not specified Results: No apparent PFOS biodegradation occurred over the 56-day period. 25 Kinetic for sample, positive and negative controls: Not specified Breakdown products (yes/no): Nore indicated Remarks: None ENVAMRD(2002)17FINAL COeNCeLUSeIO.NS Remarks: PFOS is ecalitant in the anaerobic system. DATAQUALITY. tRlaeoklciekanbsitololidtueytt:ieornKmiuiisnmeeidssc0thardtrioannsgkcitonhngecse=ynstr2t.aetmisSontwsua.dsy pnroetpacroendduacttewdicaecctohredwiantgertosoGlouobidlyL.aboNroatDoray0yPrascatmipcelse,s wTehree foREFERENCE T3hMeCsotmupdaynwya.s cRoenpdourcttceodmaptleStperdin1g0/b3o1m/00L.aboLraabtoPrriosj,ecItnuc.mbWearrcEhOaIm,04M3a4ssachusetts, at the request of the 29 ENVAMRD(002)17FINAL BIODEGRADATION STUDY CTuitllteu:re SMitcurdoybial Metabolism (Biodegradation) studies of Perfluorooctane Sulfonate (PFOS) IV. Pure oTiEST SUBSTANCE eHpdetnatdietcy:aPfelrufolruoo-ropoocttaasnseisuumlfsoanla)tCe A(1S-O#c2t7a9n5e.su3l0f-o3nic acid, 1,1,2,23,3.44,5.5.6,6.7,7,88.8Remarks: White poder, 869% purity, also called PFOS or FC-95 eee ---_ CMleotsheodd/VgiuaildHeelaidnsepafcoel)loawneddS(perxipnergibmoemntLaalbiocraalteourileast,edI)n:c BeTtwtos tsatlu.dy 1ty9p7e4siswceitreed.used (Pure Culture and "Test Type (acrobic/anaerobie): Aerobic GLP (VIN: No ear study performed: 2000 Pure Culture Studies Contact time/units: 7 days Inoculum: FourMCuusmcenpoairrnagctihepracumirenelellacluilcdtceuhsrifensgulrfaiitsoaemovactrhy.eaneAucmhseir(nifmuclnaagtnia,T(AyfTupnCegCiC,Hu1lAt2Tu0rC7eC2C)o$l9l3e4c4t)ion (ATCC) were tested: SPthraenpetroomcyhcaeesgtreicsheursyso(sapctoirnioummyc(eftien,g,AATTCCCC#1#32247732)5) O1RfecPmuFalOrtSukr.se:sTihTntehoen6ot0mesimtnLamlemdteeisdutimc,ownwacisetnhstro2ay4tbiheooanunrwsgaiostfasa-gpgipltruaoctxoiisomenat(oeSnlGyaG2)s0h.aa9nkdemrgt/aeLtb. lveTsahtsee2l5tle0sotapdtimenmgppewrriaaostru61r0emtiwh.aesoof2d6S6adg.e owTfohretkheesubttirlvoietzshesdealsndtsyccpeeaswseapcstoilncloettcetnceohtdnediv.qiauecEeuannctthirlitfheusagtravftelisaotsnkawtreadcseaip7yvee.rdfSoalarlmmnepedlceebssyswaLerrCye/cMtoSam.kpeonnToehnnetdssatyaostckt0esastonlidumt7ai.toinAonwn.eeeldyAlulsy tdhoesewattheerbsioolduebgilriatdyaotfioPnFOeSs,t systems was prepared at concentration of 1,060 mg/L approximately suite Degradation % after time: Not specified wbRiaeossturlnatontses:df,oTramhnaetdiscotlnuodosiefedsPvFwiOiaStl.hstCuPudomisenssiinwbgelheraembeipoletlrrafa,onrsMmfueocdromtraot,icoaonnnfdoifSrPtmrFtehOpiStso(my9c0e%s mdaisdsnboalparnocvei)dbeyaPnhyainnddeircaotcihoen no:f Kinetic (for sample, positive and negative controls): Not specified 210 ENVAMRD002)17FINAL Breakdown products (yes/no): None indicated Remarks: None Closed Vial Headspace Study Contact imelunits: 3 days Inoculum: Phanerochaete chrysosporium (fungi, ATCC #24725) Rrineesmaazanurrieknns.v:irTohTnehmeetnetstetaslctomnccehedanimtrbuaemtriwoaantsw2as5so0y0b.r2epammng/gwrLai.stsT-ughsleeudcioniscneub(ianStcGuiboCan)titmoenme.pdeiruatmSucrraietlew1a/21s20-2am6nL5dC,v1i/aal1s00snaessrhevaedkndetr wpolinuys vcSeoasmmsepplolsne,esnnwtesiraaelt ttiaenktoecniunolitunamcteitoenos.tnvdeHsaesyaedl3s.lpoaacAdenianwlgyadsseitspauiolrsfgewtdehreweibtnrhoottohnxoyatgneded.nceaElnaldschvciotlaelstecwftleeardsekviriaemcmceeoidnviteaedtfeaulplyatcciroeinmspmeasde CpcooennrccfeeonnrttmrreaadttiiboonnysLoCfw/eM1r,Se0.11ta`kmTeghn/eLsi,tnoactpkhpisrsoolxcuiltmoiasotenedulsyveidatltwiscteduodtsyh.ee twhaDtibefirofdsceollgiurbeaisdlaittwiyeornoeftePetFnOcySosu.tnetmeNrseodwainisintparmleapmaiersnesdiueonrcgd acrobicity and on3ldayysofexpwoeresmauintraineed. A RESULTS eee Degradation % after time: Not specified Results: The results indicated nosignificant bitrnstormation ofPROS by Phanaerochate fungi Kinetic (for sample, positive and negative control): Not specified Breakdown products (yes/no): Nore indicated Remarks: None ON ll I Remarks: I did not appear tha the fourspecies werecapableofmetabolizing PFO, DDAATYA QUALITY asRtetulediiarebsislowiletuyrbi:eliKntloyti. mciosTnchdheurcretaenwdkeiirnnega=ncco2o. rindTiathniaeclesmtweoiactkshuGsrooelodutdicooLnnacbueonsrtdartaottoriyodnoPsrsaetctatikcheenss,yInsttehmes cwlaossepdrevpaalresdtuadty.twicTehetohse REFERENCE ee T3hMeCsotmupdaynwya.s cRoenpdourcttceodmpatleStperdin1g1/b3o/0n0.LabLoarbatoPrriojeesc,tInnu.,mbWearrEeOhIa-m0,43M4a,ssachusets, a the request of the 20 ENVAMRD(002)17FINAL BIODEGRADATION STUDY "Titles The 18-day aerobic biodegradation studyofperflucrooctanesulfonyl-based chemistries, feTEST SUBSTANCE aIcdiedn,ti1t1y.:22P3e.r3f.l4u:o4r,o5o.c5t.a6n,e6s1u.lf7o.n5a.t8e8;-hmeapytaadlescoafbleuorerfoe-rrpeodtatsosaisumPsFaOlS,oCrASCHF,27S9O5/.K3"9-.3) (1-Octanesulfonic lRHaePbmoLraCartkoprsuy:r,iftPiheFedOtaSesntdiasaratsiwschlgientcwedapso3wgMdievrse.tnaanTdhateersdto,riicdgoeinnnttaiolfli3,caMatinopdnror#deTufcCetrRe-ln0tc0e0n1(u7Tm-Cb0Re4)r6.wnausmUbnpeoortnnCoArte-ecdTe.CipRtTO2hot-e0tP1hF4e.OtSesw1tia1nssg SsNtuoubdmeyixtpwtleearrneastucigaoglnecosutflsatthteihdsadtaiassnscurImeniptnaegrnicm1y0Cwe0ar%tsipfguiircviaettnye.. ofHoAwneavleysri,s irnetphortssttuhdeypurreiptoyrttobhee p9u7r.i9t%y,isAnlotreedsoatle8i6.th1e Meron -_-- Method/guideline followed: Based on EPA guidelines OPPTS 35.3200 Test Type: Acrobic GLP: No Yearstudy performed: 2001 Contact time: 15 days PsIlonarnote,cduSaltt.um4PauAClc,tfiMovrNa.taepdpTrsohlxeuidmMgaeLtceSolSlylew$catwseedde7ek3tse1r/pmr0ii0norerdtootmobetbhieeng2a2rua8st0eidomnfgo/braLsthiwinhsaesnttudhfyei.rsMteTcthoeelolesWclatuesddtg.eewTwahateesrMaTLlrSleoSaw.temdwean1st s(e2t0e0amnLd)toheftshoeliMdsLuSsSedusfeodr. inoculum. The sete sludge constituted approximately 20% of he vous `3vT5ae.ss3t2aM0de0dd.eiduMpmeesrthlTaietnesortlof(fl1amsimknsLewrpeaelrreslaplertres)pamwreaeddsiuuasmdi.dnegdMaipnmeeirnreaIrltaelrmesoafdlitmsuinmmeerpdaliluusmmesdldiuedufgmien.ewdaFiisnftpEyrPemApla.Gruoeidfdse9l7ti/en0e0d,OsPwhiThgSte fresh medium without sludge (abiotic controls) was prepared 81010, iiRnneoomccauurllukumms),:,PFTaObhSiro)et.eictTychpoeensttreoosfltssvae(msmspeillneses,raswleerrmeeidpir1eu2p5ma,mrieP.dFfNOoaSrl)gt,ehinsaenspdtoudlytye:csatbrlbsaounbnksasttalenuccdeu.glteusrcaeomnpftllraeosslkss((cmmoiimnneteraranalilnmmgee3dd5iiuommnl,.. oafppmreodpirai,tew.ere set in duplicate. Additional quality control samples (blanks) were prepared and amayocd as 2T2.h84e255teCms.uc.onTcheSentearsattmivopensswluesalesiswddenworanegses2po.in4k5de5damwgi0/tyLh.asn6duTLh1e.ofsTaamhp1edl0ea.sy3w0e0mtreesLatgvisetosalstueetldisowanteor~fe20Pp0FrOrSpempaiantreaaetndtheamipnmermaytieeulrddeiinogf prFloealmcloeovdweidinngfarhoafmivoitenhzgeer.itnhtceausttbawtvaoesrssaemlnadinctofnartioenzneetdns uantwtei-rl2e0fiandaCjluusstanemldplatenoapl1ryez%pedaa.rcaettiAiocfnteabrcyid1s8oaldinaddyspt,hhaetsnhee petaxestsstreavdctietohnsro(uSgmPheEr)s wm ENVAMRD(2002)17FINAL mCsehotanhkdaeinntoilvoinwgsoarsSohEuwsPal-ysVcaoAnlCdleCtch1tee8ndepscacpsasrSeadPteEtlhycfraoorturrgiahdngaetl.hyesiSMsePetEhnacsnauortrlreiwdqaguesan1tt0hietenaxttiarvdaecdteedxadats0corttbhseedemapnatliyeteds.cultAurewoflrasmk, snQepugeaanctttiirvtoeam-teiitovenermadoneadtleeycsutiossrinwg(asHsePlcLeoCcntIdeuMdcS-toDen)dmoosnnyisattnoerm.iHnPgL(1`TS0hI0eM)hMfiSgohDqpueawrnatfsiotraomtpaienorncaeteldiquiind eclhercotmroastporgaryapihonwiintihomnas1s cqIunoamnaptdiodfiuitneiddosnanttdhoarePpaRorOretSep,do.ttahsesiaudmdiotrioanmalmocnoimaposaulntsd,sonqluyantthieficeodncaernetrsapteicoinfioefhd ebelfolwu.orocIhnemtihcealcaasneioonfwtoh.e al2c(oNheotlh PerflCuoormopocotuannedsNlafmoneamido) chy] |NEAFcOrSonEym Alcohol | CFS`OChNe(mCic.aHlCFHoLrmCuHlLaOMT Zac0i-dcthy Perluorooctanesulfonamide)acetic |N-EFOSAA | GIF SONCHHCHICOOR) a2c-e(tPiecrafcliudoroociane sulfonamide) M356 CFSONH(CH,COOT) NPe-rEftlhuyolropoecrtfalnueorsouolcftnaantee,sulfonamide potassium salt NPREOFSuOlSfAina ||CCFF ,SSOO,KN" G Perfluorooctancate, ammonium sat Perfluorooctane sulfonamide PFOA GFCOONH," [FosA TGF. s0,N; Ntihnoeocrsuealfmeuermentisciemceosnuafbrisertmcaenodcm.epawraesdustoedt.heHporweevvieorus, wEhteFnOStEhearlecsouhlotlsf3r5o-mdaaynsEtutdFyO,StEhealvciohbaillsytuodfytchoenmdiuccsteepdiast RESULTS - Degradation % after time: 0 rfRoeerastPamlResOnsSt stAelsfuttdegvree,s1s2e8l4sd5aw5yasm,sget/xhcLeelaPlnFeanlOtyStnicwdaalsrranensoguteldtmsefadrseoummroan1bs0lt4yr-a1dt0ee8g%rh.aadtedafbeirotceaxlploysuoreabtoiomiucnlliyc.ipaMlaweastpeuwanteer Breakdown products: No Remarks: None coNce LUse ioNs wNsSauonsimltcaosostsnfedoruFrocmsPtoeRtdRhOeeaSmsrawareankrsoosbdn:ie-cmTobnhiseoidrseaugtrbda.mdiattMiasosnsssutbguadglieaenssoctefeadwPaFKsOlSi1,m0i4s-c1h08d%a.ta Tquhaelirteysurlatnskfirnogmothf2isbsetcuaduysceonthfeirsmtutdhye GLP study, but with the understanding tha good data quality obectrcs pe rs. Ea ENVAMRD(2002)17FINAL eREFe ERENCE PaLacnegeA,naCl.yt2i0c0alla.SerTvhicees1,3I-ndc.ay Macirnonbeiacpobliiosd,egMrNad.at3ioVn sCtoumdpyaonfyp.erfMliunonreoaopcotlainsc.suMlNf.onyl-based chemistries, 24 ENVIMRD(2002)17FINAL STABILITY IN WATER STUDY Title: Hydrolysis ReactionsofPerflucrooctane Sulfonate (PFOS) e TEST e SUBSTANCE seMapdlte,andt1ei-ctpa:efrluPuoeorrrofoolscuuotlrafonooencsatutalenf-copsnouitlcafsaoscniiaudtm-ep-saopltotaa(ssssCiiAuuSmms#saa2lt7t,.95o.Mr3a91.y.31a).l.2s2o.b3.e3.r4e44f.5e.r5,tr66ea.d7s.:7.P8FOS5., PFOS-patassium Remarks: 3M production lot number was 171. Test substance is a light-colored powdeart 25 C. METHOD --~ A Methodguideline followed:Based on OPPS: 35.2110. "Test Types Hydrolysasias functionof pH GLP (VIN): No Year study performed: 2001 {mT1ae:st5ti.ns3a0s,mppi8lk0ee.sp7arm0ep.pla9er0sa),tiw1oa1ns0s).apTpeTrshotxesiomrleausttueilloiynnsgSc0oP0nFswOisgSt.ecdonocSefanmtpr1.l0aemtsiiownebirunfefalehlrieecdsltadqsduuemfoprulosemsslo(lesuattmipodlnuseriasntrg6plnpactHuetsleivoelns adteg$r0adatCiofnorropuetersi.ods of 0 10.49 days. Control samples and blanks addressed potential nowhydrolyie Analytical Procedures: Samples were anslyzed by quantitative HPLOMS. xaRctetamipvaaortlkiaostn:endeTahtrios128s5tkuedCaylibwmyaolsdei.cvoidnidnugcbtyead1 f5act0Cor oinfo10,rwthodifcacheiliistravtaelihdydfroorlyrseiasc.tioRnastwsitdherAirvtehdenatiu5s0heCatwseroef RE-_S-U;-L.T.S Degradation %: Not applicable; no degradation was reported. Hallie (42): > 41 years Breakdown products (yes/no): No stRhteaatmnadtarhrekdsp:sdceuvdTioah-tefiioransntaoloyfrtdialcelarlohbyrsedseruorllvtyestdiicnPdFiacOlaSFtefcneoooncfdeenPgtrrFaaOtdSiaotniisos,ngrpoeofaotPleeFrdOtShovaoer4r1dtehypeeea6rn.doebnsceervoendppHH. lTehveel.medaineaun.d CONCLUSIONS - Thyhderoalnyatliyctihcaallf-riefseousfiPnFdOiScatinnwoatdeergriadgarteiaotnerotfhPFa4On1Soyreadrse,pendence on pH. The study indicates that the 2s ENVAMRD(002)17FINAL a`uStuhbomristtaelrsso nRoetmeadrkhsa:t tThheestauudtyhowrassaswseilg-nceodndaicKtleidm,isbcuhtrnaonkuinndgeorf2GLPf.or the reliabilityof this study. The Reviewer's Remarks: None REFERENCE LHaatbfoirealtdo,rTy.and2P0a0lcae.AnaHlyydtrioclaylsiSservRiecaesc.tioLnasborfeqPueersftlnuourmoboecrtaWn1e7S8ul.fonMaitnene(aPpFoOlSis):. MN3.M Environmental 26 ENVIMRD(2002)17FINAL BIODEGRADATION STUDY "Tile: The 35-day aerobic biodegradation study ofPFOS. TTEEST SUBSTANCE eee LL aIcdiedn,ti1t,y1:.2P2e.r3f3l.u4o,r4o.o5c,t5a.n6e.s6u1l.f7o8n,a8t;hmepatyadaelcsaoflbueorreof-e,rrpeodtaossaisumPsFaOltS, CoArSCHF,2S79O5S."3.3)(1-Octanesulfonic FwReaepsmoratHrPkarsLe:CbapPsuerFidOfoiSnedai.ps uaArwnihtiIytnoetefpr8io6mw.dCe4er%r.t,ifhiTochwaeetveeoorrfi.gAinnTaahlley3slMioswperrreoppdouurrctisttiytohnveallpouutreintwuyamstboeabrseowc9ai7sa.t9ne%od.t wniAotlthled4r.eusauTlnhtdesairPndFhtOihSse laboratory was using during this study. METHOD. a etter 8M3e5t.h5o0d4/5g.uideline followed: Based on EPA Guidelines OPPTS 835.3200, OPPTS 35.3210, OPTS Test Type: Acrobic GLP: No Yearstudyperformed: 2001 Contact time:35days sPIelntaotnlcetudlStus.mlu:PdaguAelc,,tiaMvpNap.treodxTishmleautdsegulesypceo2nl0ldee%cdtsoeodfl9i/tdh1es8w/ve0or0leuafmrleom,owwtehaedsateuorsaestdeiottneobfaposrrienapaparpertohcxeuilmMtaeutrteerlsoyfoW2dasattyhesewaabttieo4rdEeTgCrreaaadntadmteitnohtne study. The mixed liquor suspended solids, MLSS, was nt noted. 3T5e.st32m0e0d.iuFmi:ftyTmeLstofflsaestktslweedrselupdrgeepawraesdaudsdiednagpemrilniteerraolfsmailtnsermaeldisaultmsdmeefdiinuemd,in EPA Guideline OPTS iRneomcaurlukms):, aTbhiroteiec inoculum, PFOS). tycpoenstoroflssa(mmpilneersalwemreedpiruemp,arePdROfSo)r.thiasndtestt:estblsaunbkstsalnucdegescaomnptlreolss ((mmiinneerraall mmeeddiiuumm,, d`Tuepslticvaetses.elsA,ddstietriiloen1q2u5almitLy cNoanltgreolnesapmopllyecsar(bbolnaantkse)cwuletruereprfelpaasrkesdcaonntdaainniangl2y5eadmsL.appmreodpiraia,tew.ere set in SvToehlsuesteitloesns.twceSoranecmepsnlpteirskawetdeior6neutusaLekdeonfwao1sn0,d27a.6y50s52m0,m2gg,/L5,.so7l,Tuth1i4eo,nsaanomdfp3Pl5eFsOSwerine maegitthaatneodlatyi2e0l0dinrgpm8a2t.52852=m3LC. PFTeOsSt p{CT.rheepadrUaepydoznbeyrorseotmleisodtvavpelhsasfseerlosemxwtetrrhaeectpiironnecpu(abSraPteEod)ra.,ntdFeositlmlmvoeewsdisineagltsetlhoyanwpilonatgch,eeidrfnnedaeyadsefrdwe,eerezeestchviatethseswrealsicmmomameiednnittaastienwleeydrefatraodz2jenu0,s+teo7dr ptlo u1g %ofacqeutairctzacwidooalndtothdeenteprapsleudggitnhgr.ougMheathcaonnodlitiwoansedthSeEn Pa-dVdeAdC tCoItShefcecmpStPiEed ccaurlttriudrgee flcaosnkt,aisnhiankgena 7 SR ors Ari 2-(N-ethylPerfluorooctanesulfonamido)acetic CF:SON(CH)NCH,COOH) 2-(Perfluorooctane sulfonamido) idle CF,SONH(CH,COO) [NE]perfloroestanesulfonamide |N-FIFOSA__|CF;SONH(CIH) | a . ENVAMRD(2002)17FINAL uAsesderifeosroftphoesiptrievpearcaotnitornolosfwesraempnloesruinn woitthherthibsisotduedgyr.adHatoiwoenvesrt,utdhse,slaunddgeiunsesdominethoifs stuhodsyewsausdiaclss,o preliminary results demonstrated positive biological activityfordegradation oftest samples. REr FERENCE. er eee d LMainngnee,apoCl.is,20M0N1.b. 3TMheCo3m5p-adnayy.acMriobnincebaipodoelgMirasXd,.ation studyof PFOS. Pace Analytical Services, Inc. 29 ENVAMRD(200FI2N)A1L PHOTODEGRADATION STUDY (TPitRlOe:S)Screening studies on the aqueous photolytic degradation of potassium perfluorooctane sulfonate TEST SUBSTANCE sIadle,ntity1:-pePrefrlfulourooroocotcatnaenscuslufolnfiocn-aptoet-apsostiausmsiumsaslatl,t. oMray1a,l1s2o.b2e.3r3e,f4e4r,e5d,5to66a.s:7,P7.F8O8S$,-hPeFpOtSa-dpecoatfalsusoiruompotassium sal. Rpuermitayrkwsa:s de3tMermpirondeudcttoiobne l8o6t.n4u%mbbyerLC1/71M.S,Th'eH-tHesMtRs,ub"sFt-anNcMeRs,aalnidgheltecmoelnotraledtepcohwnidqeuresa. 25 C. The METHOD - M"ePthhotoodt/rgaunisdfeolrimanteionoffolClohweemdi:calsBaisneWdatoen-r DiOrePcTtSan:d In8di3r5e5ct27Ph0otoalynsdis"OAEugCuDst 20D0r0a.ft Document Test Type: Direct and indirect photolysis GLP (VIN): No Year study performed: 2001 Light Source: Suntest CPS+ or Suntest XLS lamp Light Spectrum (am): 290-800 nm Relative Intensiy based on IntensityofSunlight: 650wi aSqpueecotursusmoolfutsiuobnostfaPnFcOeS(mwaaxslraemcobrddae,dmbeatxweeepsnil1o9n0aanndd1e1p0silaomn. 295): A UV/Vis spectrum of a saturated "(Teexsptosseadm,pulneexpproespeadr,atainodn:contArloilquvoitalss)ofcoPntFaOiSninwgerSemaLdodfedaptporo3prsieaptaeramtaetrsiext.s oTfesVtOviAalsscwreerwe pclaapcevdialisn the photoreactor. Control vials were wrapped in aluminum foil, sealed in a plastic bag, and placed in the photoreactor. Analytical Procedures:Sampleswere analyzedby quantiative LC/MS and GC/MS techniques Rmoelmaarrkesqu:ivTalheentd)u,raFteio0nyovfattheerst(uFdey"waats 6274-X16m7ohloaurrs.excTehsse),tesFtem.eOdyiwaatuesredwwiethreHwayte,r,aHn:dOsc/owmamteerrc(1i:a1l (coAnltdrroilcsh)(kheputmiicnthmaetdearrika)lfoprrehpeareevdalausatiinonOoPfaPnTyS d8e3g5r.a5d2a7t0i.onreAalcltitoensssocicnculruidnedg waitsheoruietsthoefpurneseexnpcoeseodf light. Solvent, matrix, and contol blanks and spikes were ested under each condition. RESULTS ConcentrationofSubstance: Not sated 250 ENVAMRD(2002)17FINAL Temperature EC: 25 +3 Direct photolysis: No decomposition was observed. itIonnddpiirrcoeavctitdtephahstoutftofhlieycsimieinst:nidDamtauatmateoonbevasitirinomenadmteefnrtthoaeml mthhieaniFlemf,u0m,ofhmaaPltEFr-liOfxSe.sdaumTephleteosmi(enwdaiintrhecstatnapdnhdowatirotdlhydoseuivtsiaHatt,i0o2,n5)o+wfCetriheevgperoesdaitoeeadr than 3.7 years. Breakdown products (ves/no): No wtReeesrtmeeda.rankysDoi:rfetNchtoepehpvoritedodleiynctctieecdoddfdeeicgroreamcdptsotsoiiotniionpdnrioordfecutcPtpFshOodSteotlewycastsiesdnooaftboPovbFesOetSrhveweiadlsibmaoisbtsesdeorfovqneudalnuotnisdtoeafrtsitaoannry.toifntghmeatceornidli,tinoonrs CONeCoLUvSyIONNSs Nmineodaiencvaitaednetdnhactseottahfndidanirdrdeicrtdeecovrtipaihntoditioornleycottfipchtohhtaeollfoy-bsisifeseroovffedPPFFPOOFSSOaSwta2sc5oonbcCseenitrrvgaertdeiaoutnnesdretirhnaaannny3o.af7qtyuheeaorsuc.sondFietsiOonVsH:teOst;ed.matTrhiex Submitters' Remarks: The authors assigneda Klimisch ranking of2 for the data qualityofthis study. Reviewer's Remarks: None. REFERENCE - Hsautlffioenladt,e T(.PF2O0S)0.1. 3MScErenevniirnognmseundtiaclsLoanbotrhaetoarqyu.eo3usMpChootmoplaytniyc,deStg. raPdaautl,ioMnoN.fpoRteapsosrituummpbeerlruoWr2o7o7c5t.ane EY ENV/AMRDQO0)I FINAL 'BIOCONCENTRATION IN FISH "blTuietgei:llPe(rLfelpuoomriosocmtaacnreoscuhlifrounsa)t, potassium sat (PROS): A flow-through biosoncentration test with TEST SUBSTANCE e11n2t2i.t3y3:44.Pe5r5f.l6u,or6o1o.c7t8an,c8s-uhlfeopntaatde;ecmaaflyuaolrsoo-,beporteafsesrieudmtsoalta,sCPAFSOS#2o7r95F-C3-99-53.) (Octanesulfonic acid, pRuermitayrkwsa:s dSaemptleefrrtoommbe3iM6n.p9re%odbudyctiLoCn/lMoSt,nu'mHb-eNrMR21,7.F-TNhMeRt,estansdubeslteamnecnetawlasanaalywshiistetepcohnwidqeure.s. The METHODS Method/guideine followed: USEPA OPPTS 850.1730 and OECD 305 "vToelstumteypaed:diFtlioonws-tpherro2u4ghhoeuxrpso)sure with flow-through depuration phase (flow rte: approximately 6 GLP: Yes ear study performed: 2001 S(2p.e0c3i:e3s.:32Blg)uacgigll~seu7nfmiosnhth(sLeoplodmis macrochirus), mean len=6g2tmihm (56-66 mm); mean weight: 2.7&0 `Supplier: Osage Catfisheries, Inc. Osage Beach, Missouri `Concentrations tested: 0,0.086, 0d 087 mg/L. 3U5ptdaakyes dpueeritood:fi6sh2mdoarytasli(ty)0m.g/L0exp8osur6e); 3 days (0.087 mg/L exposure- this exposure ended after Depuration period: 56 days (0.036 mg/L exposure); None (0.87mg/L exposure) AInnatelmyattiicoanlal,moLntidt.oriWnagt:er sAanmapllyessesweorfe dteisltutseodluatnidonasnaalnydzedfibshy HtiPssLueCs wwietrhea pseirnfgloermqeudadartuplWeilmdaisfse swpietchtiroimpeteriqcuadedtreucptiloen.masTsisssupeectsraommpeltersicwedreteechtoimoon.geniWzheed,n edxettrearcmtiedn,indgiltuhteed,coanncdenatrnaatliyoznedofbythHePtLesCt ssuebds.tanNcoe aintttehmeptsawmapslesm,adtehetosaqmueanainfdymoonsttheprboamsiinseonft ipnedaikvidrueaslpoinssoemefroircpceormfplounreonotcst.anesTuhlefoLnaOtQe wwaass c0a.0c5h msagm/pLlefosriwnacteecracinhheintsirsetusduy.bmiFtotredtissasmupelsea,mpolfedsi,fftehreinLgOweQigwhats, cwaalscuelxattreadctoend awnitihnodui!viadnuaaldjbuasstsmefnotr 10 constant weight ARneacloyvteircaylwreassultesxcweellreenntoitn cboortrhecwtaetdefro anpdrofciesdhurtaislsureesc,ovrearnyg.ing from 84.9 to 122% of fortification levels. pCooilnltesctdiuorninogftthiess6u2e-dsaaympulpetsa:ke pFhiasshe.werTehecyolwleecrteedeuftrhoanmizteesdt,cbhloatmtbeedrdsryb,yweriagnhdeodm, saenldecmteiaosnuraed1.2 tFiimseh m ENVAMRD(2002)1 FINAL tT`iwhseseruee.ftrhaTechtnieornirsnesmweaedirwneiitnihgndtdiiivslisudutueiaoilnnlcywlauwtdeeiri,ngghbesdik.itntweTahdsercyhaeogaadni,n,sfianins,dtdoabdneiedssreevdcieitsbecdldeeriainstwoseurcede.ibcloensaindderneodnefodibbee nfornacctdiionbsl.e anStpoapntaeirdseitnbitciaesltpeamaretdsty.-hsotdaSstte:eaddiyWv-ihsdtaealtdeebbyfiitsohhceocnmocneecnaetnnrtawrtaaittoeinornBcsoCnwFceenrvtearlacutaeiloscnu.claaltcTeuidlsasbtueuedsecfdornocoemnntttrhhaeetitsoinusssmuewoecfroentchceeonntesrdiaidtbeilroeendsana1d0t dbieffarteanptp(ar2e0n.t05s)t.eadTyi-ssstuateeciof3ncenotrramtoiornes cwoenrseeecvuatliuvatseedtsfoorfntoirsmsauleitcyonacnednthroatmioognesnewietryeofnvoatrsiiagnncifeicuasnitnlgy tthreanSshfaoprimreod-Winika'ns atetsttemapntd tBoartcloertrte'csttetsh,e rdeastpae.ctivMeleya.n Ititshseudeactoandciedntrnoattmioenes wtehreeastshuemnptcioomnsp,ardeadtauwseirneg ANOVA and Dunnett's test t`BThIheeOeFkdiiAnbCeltei,ics naboinnoeocdniolbnilnceee,anrraanpdtaroawnmhfeoatlceetrorfeis(sthBiCmeaFxtKpe)o,sroeuudtpittnaoe,k0ew.h0rai8t6cehm(keg;s)/t,LiamaPntdFesOdeSrptuuersaictnoignosntBarInaOttesF(AfkrCyo)mcwoermSepetucatoflesceusqlouafettnewdtairfeao.lr time-concentration data. These ate constants werethenused to calculatae BCFK (BFK ~ kk, `"Teexsptoscuornedivetsisoenlss.: OSnteaivnelsesseslspierlcaoqnucaernitara(ti1o0n4 wLa)sfuisleedd. wiTthhealpopardoixnigmaratteelywa$s00.L48sogluftisiho/nL wdearye (u9s0edfiassh apenrdvhessoel.uoTfrhdeasrfkiwshiwtehrae 3f0edmfilnaukteeftooadns(iZtioegnleperriBordo.theTrhs,eIlnic.g,htGairntdennesirtsy,aPsA)2a7n8dlguixvaetn1th6ehsouurrfascoeoflfighhet negative control vessel at est initiation. t"Towpo-dsotwonckmsiolxuteitroonsaiwderinetphreepsoalruebdilaitza1t0ioannodft1h00 tmegst as.u.bLst.ancSet.ockAfseolrutmiioxnisngw,ertehessitroecdkswaipthpeaanreedlecclterairc awnedrecoilnojrelcetsse.d inSttooctkhse wdielrueteprrmeipxariendg acthaapmpbreorxsimaattaelryatweeoefkl3y.5inmtLeirvmailnsudtueriwnhgetrheetuhpetyakweerpehasmei.xedStwoictkhs adiplpuetaironedwatcleerarataanrdateocoflor3l5e0ssm.LmiTnhueteditloutaicohniewvaettehre dweassi.remdotdeesrtaceolncye-nhtarradtiownesl.l Alwlatfeirnaweistthsoslpuetciiofnisc dciosnsdoulcvteadncoexyogfe3n13leuvemlhsosralncgme,dhfarrodnme6s.so4-f8.613m0gm/Ly,,thaelktaelmipneirtyatoufre17r5a,nagnedd pfHroomf21..17. tD0ur2i2.n0g thCe, easndt tthhee PH ranged from 7.910 8.2 Remarks: None RESULTS Bioconcentration factors (BCF): 0.086 mL. apparent steady-state BCF: Edible: 484 Nonedible: 1124 Whale fish: $56 0.87 mg/L(study ended prior to achieving steady-state) BCF: Edible: 136 2 ENVIMRDQUO)ITFINAL Nonedible: 356 Whol fish: 275 BIOFAC Estimates using 0.08 my/L. exposure): Berk: "0Ti%mectloearreaancche: Edible 1866 146 days Nonedible an 133 days Whaleih 3614 152days (PVRalOuSesCoanrecefnrtroam4tiionndsiviinduTailssfusesoatfcBalcuhesgaimlpElexppoesreiodd()0 0.086 mg/L: OUGpithaokwdsea)y_||F0a.1b6l7,e0.s1s5w5,,0m1g4/4k,g ||N0o4n1c5di,i0e5is1s5u,e,0m40g1/47g9,7||W029h5,0o3fi5sl1h,ceo02n3c6,m3i67 [|[omew018meoat [sim mm 0137036.707.200.201 439.550.|53491,740.045159.4738 [21i [10171.4,152007.,113279,.112066 [27130.22.10026..315139..315226 [202.169.260.246 [333,227246,244_[233,184, 198. 185 3s3 [212863..21773.72.3283.920.6251 4691..4,,74530.76.2605.35,95.719 ||936533..523952.445646..444019 E[ i) 42 1323736..326523.,2319.02..320766 | 640.681.544.796 50.1,49.4,40.9,56.3 56 483.389.4038. 1[0112,21,1924,120,57,30961046 970160..792176..169363..773415 | aDaeypuration . 21 83318316, 20 |0 10 481 |9$0828 3..700.%1.661 15.61..8851130| Sao 211.376,329.312 571777..880063..788534..882415 |[S3L1i6..661645..661508..662212 EY ENVAMRD(2002)17FINAL (PVFalOuSesCoanrecernotmra4tiionndsiviinduTailssfuisehsoatfeBalcuhegsialmlpElexppoersieodd.t)o 0.87 mg/L: |Uptake day | Edible tissue, mg/kg] 104 hours) Nonediblisesue, mg/kg 3.52,437.420,406 1 3 416783..615598..515960..526048 ||319131,,42104,243,81.335,11281 920,440,577,468 | 100,102, 102. 120 2[11a 8797..411,1871,6,19004.,71.07233 ||210717,,227087.,224465.,221249. = "Sampling ofish stopped after Uptake day 22889d,3u72to,3mo2r0t,al3i6t1y | Wholefish conc., mg | 2[.87010,.31.0098.,120824.,024879 | 7304.95,,7307.07,.8345.35..3899.15 141, 157, 180, 158 205,267,232. 263. ddReueprmuiarnargtktisho:endpehpTauesrseatst)i.oorngAaptnhia0ss.em0.86mAomtraL0l.i,8t7yomngwe/aLsf,isnmhoodrniteealdiinatyftewhreas4n9efrgdsaattyinsvoetaencddonootnnroedldi(aed9duaryainfndtgercbo5on9tthidnautyehs.de tuhNprtooanukgeehddaianeydd 35 ofthe uptake phase, at which time all fish either ded or had been sampled. Was control response satisfactory: Yes Statistical results: None CONCLUSIONS eet feretenrets eet terete waPteFarOieSnenodbtioosntcaotDnicasteyinct4arl9alytoesrdigtnihinefitfchiaesnhttleiysxsdpuioefssfeeodreftno,bl0Pu.egO0i8lS6lmcsgouannc.feiins/thLr.adtuirAoilnntsghaotpuhpgiesharsDteauddyty.o4b9e,A5spt6pi,lalraiennndctr6e2satsetiaindsgys-udesutrraitenesgiwtdhauises 4ti5m4e..11A2p4p,araenndt8s5t9e,adrye-sspteactteivBelyC. values for edible, nonedible, and whole fish tissues were calculated to be `PaFndOwShodelpeuriasthedtisslsouwelsy.werTehe14B6,IO13F3A,Canedst1i5m2atdeasysf,orrtehspeetctiimveeltyo.reach S0% clearanceforedible, nonedible, Submitters" remarks: Theauthors assigned this studay Klimisch data reliability ranking of| Reviewers! remarks: None e REFERENCE ee ee ee PrfDorljooetwca-trt,hnrKou.,umgbheVrabin4oH5c4ooAvn-ec1ne3,n4t.Rr.a,t3oaMnndtCeos1tm.pwKairntuhye.gbelruS.etg.ilP2la0u0(1l.L,eMpNoP.meirfslmuaocrrooocchtiarnuess)u.lfoWnialtd,lipfoetaIsnstieumamtisaolntal(,PFLOiSm)it:edA. 255 ENVIMRDQ002)17FINAL Annex 6. Robust Summariesof Toxicology and Human Biomonitoring Studies PHARMACOKINETIC STUDIES Titles Absorption ofFC-95-1i4Cn Rats aftear Single OralDose (1979) TEST SUBSTANCE Identity: Potassium perfluorooctylulfonate, CAS 2795.39-3 sRpeemciafrikcsa:ctiFvCi-ty95is-0144C59(c+arb0o.n0-0184ulCaibmelg.alpThhain(-0lasuylefruranadtocmo,luRminkecrhIrsoomtaotpoegIrnavpehnytosrhyoNwuedmbteherF4C42-)9.5-T1h4eC t(oSybnethaetsilesasdtes9c9ri%berdadiinoJcohhemniscoanllayndpuBree.r,Th19e79F)C.-95-14C was found to be suitable for metabolism sudics. METHOD Method/guidelinefollowed: NA Test types invivo Species/straint/yepeeolrl line: rat, Charles River CD. Sex: male Age and body weight range ofanimals used: weeks, bw mean 285g (range 243-315) Nuofmanib malsseex/dr ose: 24 Routeofadministration: oral Vehicle: 0.9% NaCL solution containing 1.2 mg FC-95-14C/2.0 mi Doses: 4.2 mukg average,single dose rEexdcrbeltoioodn rceolultse,s,plbaosdmya,fluuriidnse,,anfedcetsi,sssupelseemno,niditgoersetdivaendtr/aocrt spalumsplceodntdeuntrsin(gessotpuhdayg:us, stomach, small intestine, large intestine, and colon), and carcass Statistical methods used: mean, log mean concentration versus time last squares line. RMaettshwoedrreemcoanrdkist:ioned to individual metal metabolism cages for 24 hours prior to dosing. Rats were awleliogwheedd firememeadcicaetsesltyo bPeufroirnea GberionugndgiCvehnowa asnindglweatoerralbedfoosree oafndFCa-f9t5r-d1o4sCi.ng.ThEeacdhonsoinn-gfassotleudtiroant wwaass p`mreecphaarneidcably ashdadkienrg,~a2n0d0cmegnotrfifFuCgi-n9g5.-T1h4eC tsou0p.er9n%ataNnatClw,ass hreamokfvoierdnonagnedhaulsfehdoufroratdomsoidnegrastoelutsipoene.dTnhea 2ca.rb0onc-e14glcaosnstseynrtionfgtehe(Trdyolsoinn)gfsiotltuetdiownitwhaas sdteatienrlemsisnesdteeblyidnitrucbtatcioounnttiubneg.. TRheecodvoesreywoafstodteallivcearerdbowin1t.4h fsarmopmlessertiosacocfoFunCt-9f5or-1la4bCelslpioksedd,urbilnagntkhebieoxlpoegriicmaelnstaalmpmlaensipwualastiuosnesd. to adjust the recovery of the test 256 ENVIMRDQO)I FINAL GRiramtmoseudpwiseartoefetlhaynreetsertahnresattfisewzrerederdweisttoahcaidifheietchpeyaldrbitnyhiezeerxdsaantnudbgeub.lionoaPdtlowanasmsatad1rw,aa2ws,n6p,frre1op2m,ar2te4hd,e 4pd5er,socm9ep6nt,dlaiynndgbya1o4rc4etnahotrofiuferusagacpthoisotnra.tdoasneIdn. a(cdeadsciohtpoihfoangtuhset,otshprtleoaemsaamctahs,aisnnmdtahlerle2di4ntbealsntodiond4e,8clheaolrulgr,esi(pnoottesaslttidunores,ienae,gnrdooctuoapllsonf)fo,ercaecsna,drbsropenlm-ea1ei4nn,adneadrlioygsefisstc.iavrecarssawterpelosyavSeodntfernotms -R0 ESULs TS lADeefatstatei9lrae5ds%iornfgelsetuhoser:taotdaolscearobfoFnC--1945i-1s4ysCte(mmiecaanlldyoasbes,o4rb2edmgat/k2g4)hionursso.luTtihoen htoaglrofupfsoofetlhirmeienamtaiolneorfattso,taalt carbon-14 from plasma s 7.5 days. "f1Te.hc5ee5sd%iagnoedfsttdihivegeedtsrtoaiscvete aartnad2ct4cophnlotuuesrnstcsoancntodenntt3as.i2nies4d%5o%ant o4tfh8ethhaoevuerdrsoa.sgee.,At3S42o4m5e%hooouffrstt,hhietshde5o%sme.elainkTehlseyumrmeepoarfensteofnettcssalcsaeyrxsbctroeenmt-iic1oa4nlliiyns daoafbtsae.oxcrrTbehetudisoc,na.ratbTolnhe-ea1s4da9ptr5ae%sferonoftmtethihetehFe4rC8-in9h5oth-uer14dpCiogsedtsotdsioevseewtagrsarcotaubtpsoiosfsrurbeetsdsoarfrrieoncmtohsneoslidusittgieeosnnttiavwfeietrrhaatdchmeicno2in4sttehrnoatutsrioapnosa(t0rednsoouslnet sfmaoastmtpaelsdreealatil.aibqluTeohatess mwtaajhsoordtiphfoeirrctutiliotsns0uoefadtshaseuarresa.idnicoTeahclteairrvegietiysvroselocumomveeerehexocdmrwoetagiseonnfaotouefnsdtowtieanlrtehceanrecbcaorecnsa-ss1as4.ryiTan hnuderichnaoermco(a1gs-es3n9de4aittdayeayro)ef "paTenhrdecepsnlptalseoemfnatshweferrdooemscetohmiepna2trh4eedhw.ohuorTlahenoedrmg4ea8annhworauarstipo~o0so.tf2ed%o.dsebTlrhoaetosdcwocenecrleenttaornpaatlliayozsnemsdaoffcootrnocoteatnltacraactraibrobononna1-t41244incaronenddtbe4ln8o,ohnodudrceshlliess s0e.l2e5ctainvde r0e.t3e9n.tiroensopefcctiavrebloy.n-1T4huisn,readt b2l4ooadndcel4l8s. hours afear single oral dose of FC-95-14C. there is no "tcTohhneeceehnrtaarsaltaifton1o,vf2e,resl6u,ism1it2n,iamt2ei4o,fn4o3rf,rho9em6s,epaldnaadstam1aw4awsahsopuldroestttaeedrf.mtiTnrehdesibnlegyalseatnoasrlqaylusaidrsoessoefolipfnelFatCsh-mr9ao5u-sg1ah4mCpt.hleesiTnfhdreiovlimodgugarolofuppmosienaotnsf cfpolrnaocsmemnat2or4fatt0iootna.1l44c`Thhaeouhrraslbfiaoforfeeontrhfea-seseli1indmga4iltneaatofiriaotlns dtfohrseoemeoqpuflaFtaiCso-mn9a:5s-C1p417C9=hiso1ul5ro.sw6.(57.(5-0da.y0s0)3.87)Thuwsh,ereelimCipnatisiopnlfarsomma Metabolites measured: none. E coNcLe UO stoNs ee agree REFERENCE eee LU APabuslo,rpMtNi.onoPfroFjCec-t95No-.148C90i3n10R2a0t0s.afJeohransSoinn,glJeDO,rGailbsDoosne,.SI,RiaknedrOLbaebro,ratRoFr,ieOsc,toInbc.e,rS2u6b,si1d9i7a,ryof 3M, St. 27 ENVAMRD(2002)17FINAL "ITnittlcea:veEnxotuesntDoasnedoRfouFtCe-o9f5E-x1c4rCe(t1i9o7n9a)nd Tissue DistributionofTotal Carbo1n4 in Rats after Single TEST SUBSTANCE Identity: Potassium perfluorooctylsulfonatc, CAS 2795-39-3 sRpeemciafrikcsa:ctiFvCi-ty95is-0144C59(c+a0r.bo0n0-814uClaibmelg,alTphhian-tolasyuelrfuarnadtocmo,luRminkecrhIrsoomtaotpoegIrnavpehnytosrhyoNweudmbteherF4C4.2)9.5-1T4hCe. 1(0Sybnethaetsilesasdtes9c9ri%berdadinioJcohhnesmoincaalndpuBree.t,Th19e79F),C-95-14C was found to be suitable for metabolism studies. eee Methodiguideline followed: NA es ttre Test types invivo Speciesstrain/eel ype olin: rat, CharlesRiverCD. Sex: male `Age and body weight rangeofanimals used: weebkwmsea,n 258 g (range 262-303) Nuofmanimbals/e sex/drose: 6 Routeof administration: iv, via tai vein Vehicle: 0.9% NaCL solution containing 12 mg FC-95-14C/m20l Doses: 4.2 meg average, single dose uErxicner,etfieocens,roluitveesr,pbloadsymaf,lukiiddsn,eya,ndlutnigs,ssupelsemeno,nibtoonreemdaarnrdo/wo,rasdraemnpallse,dkdiunr,itnegstsets,udmyu:scle, ft eye, brain Statistical methods used: mean, standard deviation MRaettshwoedrerecmoanrdkitsi:oned to individual metal metabolism cages for 24 hours prior to dosing. The ras were aanlelsotwheedtifzreede waictchesdsiettohyPluertihnear,GtrhoeunngdivCehnoawsianngdlewaitvedrosbeefuosrienganad3.0afecrc ddoissipnogs.ablEeacphlasaitc wsayrsinwgeeigfhiettde,d wdaiytsh. a 2A6UgSaugdeays172p"osnteeddolse., tUhreinretsanwderfeeceasnewsetrheeticzoeldlcwtietdh adtitinhtyelrveatlhserf,orbelaocohd owfasthedrsaixwnratfsrofmort8h9e descending aorta, animals were sacrificed by exsangination, and tissue samples were collected. RESULTS DBeyta5i9leddaryessulptoss:t dose, mean urinary excretion was 30.2+-1.5% of total C-14 administered. Mean ctoumumleaatsiuvreefeacfatlerex6c4retdiaoysn.wasAC12.d6a+y-1.829%,.meTahne atuitshsouresCn-o1t4tchoancreandtiroaacttiiovnescaobnotveen ionnefecuegs wFaCs-19050.1l4oCw 258 ENVAMRD(2002)17FINAL demiqufusficevreea.nlceseknitinns,Cwb-eo1rn4eecaomsnatrferonlotlowo,wfss:aunbdlcivusetrp,aicn2ee0no.6uh;sapdfloacso(mn0ac.,2enu2tgr./a2gt)ikoiandnsnderyaa,bndg1oi.mn1i;gnaafnlrdofmlutu0n(.g<2,=110.01..00.86Ogtuihggeg)r. tiVsTsehureeyrselsiuwtcaehsCa-3s c1o4ntwaaisnefdouansdubisntawnhtoilalepeeyrece(0.n16tuaggeo/fgd)osaendanto$d9etdeacytsapbolsetCd-o1se4,w2a5s.2f1ou%nadndin2b.r8a1in9.%, Orenslpyectfiivveelry.andThpelalsomwa tlehveeslesoorfgarnasdiwoahcetnivihtoymofgoeunnidzfeodr.kidney, lung, testes, and spleen are due in pat to blood stil contained in CMoelalenctEixocnrePteiroinoodfTota Carbon-14PienrcUernitneDoOsveer Time: 0(D0a5ys) During Pe0r9io1d 01521 512d1 3243 019033 5as6 007813 7675 007756 95.910 00.66s8 1101121 059 038 1213 1514 003359 13.15 15.16 005s1t 11761187 004.38 11982119 008349 2225 006768 22.52297 006688 322.3362 0180s6 3064403 007959 48.4570 006982 S0S45547 006718 671.-6619 017590 705..7580 2T0o8t Tonal 302 Mean ExcretionofTotal Carbon-14 in Feces Over Time 29 ENVAM/RDQ002)17FINAL (CoDlalyesc)tion Period DPuerricnegntPeDroisoed 0050151 00504429 2132 00769459 a3sa o06s5m6 667 00358180 78 89 00442812 91.01101 00337807 2213 0032190 114511s 00228716 1156:1176 00128172 11781189 00116239 a19n21 00330112 52227s 00220682 2232 00222032 36325306 0155320 Tporueld os126 "The radioactive content ofthe feces was 00 low to measure after 64 days. `Metabalites measured: none. CONCLUSIONS agree REFERENCE GIEnixtbtrseanovtne,annoSIdu,sRaDonoudsteeOoboeffr,EFxCRc-Er9e,5t-iDoe1n4caeCn.mdbTeRiirsks2eu8r,eLD1a9ib7so9tr,raitbourtiieosn,oIfnT.,otSaulbsCiadribaornyo-1f43iMn,RaStts. aPafule,raMNSi.nglJeohnson, ID, 0 ENV/IMRD(2002)17FINAL A"Tmitmleo:niChuomle1s4tCy]rPaemifnleu-oErnohoaentacneodaFteecaolr EPloitmaisnsaituimon[o1f4CCPoerrbflouno-r1o4ocintRaantcssualfftoenraAtdem(i1n9i8st4r)ation of TEST SUBSTANCE PIdoetnaisistiyu:m perfluorooctanesulfonate (14C-PFOS) Ammonium perfluorooctanoate (14C-PFO) R1e4Cm-aPrFkOsS:: sp act 0.46 uClim, radiochemical purity >99%, 14C-PFO1:45Cpalacbetl i0n.31PuRCOlSimies.adrjaadcieoncthetmoiscuallfuprurity >98% METHOD AAA A ALE1 Method/guidelinefollowed: NA Test type: invivo, Specics/srain/tycpelorl line: at, Charles River CD. Sex: male Age and body weight rangeofanimals used: 12 weeks, 300-342 g Nuofmanib malsseex/dr ose: 5 Route of administration: iv Vehicle: 0.9% NaCl, 2 mat PDootsaesss:ium[14C]Perfluoroactanesulfonate (PFOS): 3.4 mgkg mean, single dose,0.56mg/m `AmmoniPuFmOS[1c4oCn]tPreorlfalnuirmoaolcst:a3n.o5atmeg/(PkFgO)m:ean13.3mg/kgmean, singe dose, 2.1 mg/ml PFO control animals: 13.5 me/kg mean UErxicnree,tipoanstraou,terse,d bbloodoydfclluild,s,iavnerd tissues monitored and/or sampled during study: Statistical methods used: mean, standard deviation,Students test IRMraesttsphwroiedorrertehomoaurrsekcesedi:viinnigndtihveidufalluosrtoacihnelmeiscsa-sltse.el mTehteabroaldiisomlacbaegleesd acnodmfpaosutenddswitwhefreeeaadcmicneissttesorewdat5er sfoirng2l4e winetrreavdeonsoeuds wdiotshesea(clahtecroalmptoaiulndve.i).FivTewaotsmlfroofmdcoascihnggrsoouluptiwoenrewafsedadcmhionliesstteyrreadmitnoee(adcrhiedrata. ndTegrnourantds w`raiedstmihinnc7ihs6ot2lr0ae)ts,itoynr4ao%mfiniPenF.OfSe.Idn(CoPorundrteirrntoaloLaralabtlsCwoheworwec)ao,dmpmfaiornrii1ss4otnedoraeyfdstarhafedeirroaladadibmoeimlneeitdsrtifrclautroeisrouonloctshfeomPinFcOaalnabnuadtbfswooelru2et1endobaatsyirss,eatftetheder 200 ENVAMRD(2002)17FINAL ri1na4tdeCiro-vlPaalFbseOlfSodraodsimenisdnwiisvetirrdaeutailnoontr.aasdAjitunsttcheaedcshfeogtrriimoneudspi,vuirndatutisallweb14roeddyaanywesesitaghfhtetetsri.ze1dU4rCwi-intPehFaOdnidaefdthemyiclneisessttahremartpialonendsaewnxedsrae2n1gcoudlialnyetscetaeddtbeayrt cdernatwriifnuggatbiloono.d Lfirvoemr wtahes cdoelslcecetneddinasgtahoertwa.holePloarsgmaan aannddstroerdedblfrooozdenceulnltsil waenraelysperepared prompily by RESULTS ee tee lL (DAfe7tt5ea5ril2+e1+d 5dr.ae0sy)uslwtosaf:sc9h.o5l-eostlydrtahmeinmeetarneaptemrencte,nttahgeemoefadnospeerecleinmtaigneatoef v1a4Cf-ecPeFsObSydcoosnetreollimaitnsat(e8d.0vi+a- f0e.8c)e.s cAhfotleerstaydrjaumsitnmee-nttratfeodr),thteheaammoouunnttsooff ccaarrbboonn--1144. reexmcarientiendg itno buerienxecr(et1e8d%arefor19c%ofntorrolcshoalnesdty$ra%minfeo.r t2r1eadtaeydsatrseparnesden7t4s%1f1or%coanntdro4l0ra%ts.ofAtfheerdo1s4eC-fPorFOcShoaldemsitnyirsatmriantieo-nr,eattheedmaenadn clovnetrrcoalrbraotns,1r4espceocnttievnetlya:t Mcheoalnestpylraasmmianeatnrdeatrmeedntbiood cell carbon-14. concentrations are significantly lower ater 21 days of (A4f3t.e2r +145d.a5y)swaofsc9h.o8l-eosltdyrtahmeinmeeatnreaptemrecnetn,tatghee omfeadnospeeerlciemnitnaagteedofvi1a4fCe-cePsFObydocsonetreollimriantast(ed44vi+a f1e.c0e)s cAhfotleerstaydrjaumsitnmee.nttratfeodr),thteheaammoouunnttsooffccarrbboonn--114.4 reexmcarietneidngitno ubreineexcr(e6t7ed%arfeor1c6o%ntfroorlschaonledst4yr1a%minfeo.r t1r4eadtaeydsartespraensden2ts84%%foarncdon8tr%oolftrahts.e dAosferfor14cCho-lPeFsOtyardamimniinset-rraetiaoend,atnhde cmoenatrnolliavetr,carresbpoenct1iv4elcyo.nteMnetasnt tprleaastmmaenatndred blood cell carbon-14 concenirations ae significantly lower afer 14 daysofcholestyramine TCraerabtomne-n1t4GCrooncuepntration (exLpirevsesred asugeqP/glatsismsaue or ml lidR)ed Blood Cells 14CC-hPoFlOeSstyramine ~~ 94%-1L6* 09+0.1 030010 Control 356456 69006 1804 HCChPolFeOstyramine Control 2232621 s1i47e68 4128002740 "Significantly different from control values (p<0.05) d"Tohseinaugtahnodrsthceonaccltudtehatthcathotlheeshtiygrhamcionnecetnrteraattmieonntoefnh1a4ncCe-sPfeFcaoOlrScl1i4miCn-aPtiFoOnoifnlcivaerratb2 otobny3n-weea1erkl4sya1f0e.r fold suggest that there is aconsiderable enterohepati circulationof14C-PFOS and 14C-PFO, Metabolites measured: none CONCLUSIONS agree re.) 0 ENVIMRD(2002)17FINAL REFERENCE J1o4hinnRsaotns,afJt.D.erGiAbdmsionni,sStIr,atainodn oOfbAmemRoErn(i1,9u8m4)(.1C4hCo)lPeersftlyuroarmoionceEtaanonace eordPFoetcaasliEumlatioonFCarbon [14C]Perfluorooctanesulfonate. Fundamental and Applied Toxicology 4, pages 972.976 MS1eN4e.3-a1ls4oCJionhantsosnw.itJ.hDc.,hGoilebsstoynr,amSiIn,earndamObeenr.RPEro(j1e9c8t4)N.o.En8h9a0n0c3e1d02e0li0m,inRaitkieornoLfaboFrCa-t9or5i-e1s4,CInac.ndSt.FCP-au, FS ENVAMRDQ002)1 FINAL tOThrieatleP:r(eGsaevnacgee)anPdhCaornmcaecnotkrianteitoincoSftPudeyfolfuPoFrOoSocintaRantes,sAunlafl(yoPtniFcaOatSle)LaibnoSreartuomr,y RLievpeorr,t,UrDientee,rmainndaFteicoensof Samples RTEST SUBSTANCE Identity: Perfluorooctylsulfonate, potassium salt, CAS 2795-39-3 ll] Remarks: Purity 86.9%, Lot #217 METHOD iA "MTehtishosdt/ugdyuiwdaeslicnoendfuocltleodweidn:compliance with United Sates Food and Drug Administration (FDA) Good Laboratory Practice (GLP) Regulations 21 CER Part 58, with the exceptions noted on page 3 ofreport. Test type: invivo Species/strain/eel type or line: rauSprague-Dawley pregnant Crl-CD(R)BR VAFPlus(R) Sex: FO: female, FI: both Age and body weight range ofanimals used: 60days, 200-225 Numberofanimals/sexdose: FO: 16, F1: male and female pupsfier Routeof administration: oral Vehicle: 0.5% Tween(R) 80in R.O. deionized wate, dosage volume ml/kg pDroisoerst:o c0oh(avbeihtiactlieo)n,,0.a1,nd0c.4o,nt1i.n6u,eadntdhr3o.2ugmhgdlakyg/1d4ayoridnavyol2u0moefporef$summiekde,geostnacteiond.ailyOnbleygitnhneiFngo 4f2emdaalyess were dosed. FExOcurreitnieo,nfercoeust,esse,rbumo,dyfivfelru.idsF,1alnditaisnvsduesesermuronn.itored and/or sampled during study: Statistical methods used: mean and standard deviation b`eMfeotrheodmarteinmgaraknsd: atSgeersutma,tiounridnea,y a(nGdD)fec7,esGsDpec15imaennds GweDre21.collLeicvteerd sfpreocmimaednusltwefermealcoellreatcste(dFfOrdoammFsO) fdraommsfeattutsecrsmoinnatGiDono2f1. thSepestcuidmyen(sGDwe2r1e).sen'tA 0tottalheo3fM$4 EpnovoiireodnmseenrtuamlaLnadbolriavterorsypaecnidmtehnescwonetrreacctollleacbt1es0d be analyzed for PFOS. 24 ENVAMRD(002)17FINAL RESULTS ADvetearialgeed rReessuullttss: for the Analysis ofSerum Samples (ug/mi) Dayodam O0m0g72k3g Ol8m89pke Od0m7gkg Lom16e0ke DDaayyl7sddaamm 000.912266 $78802 L40s9 115564 DaDayy221lFdetmal 000.172154 94.2047 324632 110316 S23m1gske 217055 1i6s5s DAvoesreaGgreouRpe.sults for the AnalyPsiFsOoSfLCiovnce.r(Suagmlpe)les~~(GPDFO2S1)Cone. (ug/g) 0(mgkglday) Female02Ad8u8lt (FO) Fetal0L1iv6e9r (FI) 0014 21027 379036 3126 631470 82607 Average ResultsOmfogrktehe AnalyOsiismogfkUgrineOSdammpglkessfromLFo0mgDkames Dayo <L0Q 0097 032 0959 321m5g3ke DDaayy?Is 0<0L000QS 0000608250 00532068 016102 0136603 Day21 00194 00514 055 271 [x Average ResultsOmfogrktshe AnalyOsilsmogfkFgecesOS4ammpglkesyfromLFOmgDakmes DDaayy?o 00001358s0 00449909 224126 190.139 DaDayy 2I1s 00003322 o0d6l6s2 223939 9194 322m9gke 23005 201 (tInhGeDg2ef1ne.etruastelhs,e. tlehPveerFleOswSaosfwaaPsFdOaolSsseo-wroeebrlseaetmrevduecidnhci rheitaghsheeericnoinntthretohlleedvlaeimlvsseor,fahaPnFweOilSnl tiahnsettshheeerlcuiomvnetrfrooarlndtfheestuedsreausn.mso.fOnTthheseacdrlaiemfvisecelaonondf GPDF2O1S. reImnaitnheedGfDai2r1ly fsetteuasdeys,inthtehelesveelruomfoPfFtOhSe dianmtshefrsoemruGm Dwas-- gGenDera1l5l,ybuctomtphaeralebvleelstodrtohpepeJdevealt observed in the dams, whereas the level ofPFOS in the fetalliverswas well below that een in the dams. ARessuslttatsedReimnatrhkes:report. "It is not possible (0 verify true recovery of endogenous analyte from tissues swpiitkheoustturdaidesi,o-ilnadbieciaetdertehaftertehnecedamtaatearriealq.uaTnthietaotnilveytmoeSas0u%reomregnrteaotfersccuracy availble at this time, matrix Metabolites measured: none 265 ENVIMRD(2002)17FINAL CONCLUSIONS ``aCnodncsleursaioofnsa:l UfnemdaelretrhaetscdoondsietdiownisthofPtOheSprdeusreinntgstthuediiens-,ifPeFpOhSasweaosftohbesesrtvudeyd. iAndtdhietiloinvaelrlsy,,urPiFneO,Sfewcaess observed in fetalliver and serum taken during gestation from the same groupoffemal rats. Agree REFERENCE SAntauldyytiTciael:LaObroarlat(oGrayvaRegpeo)rPthTairlmea:coDkeitneertmiicnSattiuodnyoofftPheFOPSreisnenRcaetsand Concentration of 3PeMrfMleuodriocoacltaDneepsaurltfomneantteS(tPuRdOy:S)T-i6n2S95e.r1u2m,, ALirvgeurs, IUnr-iLnief,eaSntdudFy:ec4es31S3a-m0p1l3e,sFACT TOX-110, 3M Laboratory Request No. U2849, 3M Environmental Laboratory, May 4, 2001. 266 ENVIMRD(002)1 FINAL TOriatle:(Gavage) Phammacokinetic Recovery Study of PFOS in Rats, Analytical Laboratory Report, FDeecteesrmoifnaCtri:oCnDoBfRtheVACFo/nPclenutsratiRoanisofExPpeorsfleudortoooPctFaOnScsuvliafoGnaavteag(ePFOS) in the Serum, Liver, Urine, and TEST SUBSTANCE 1] Identity: Perfluorooctylsulfonate, potassium sal (FC-95), CAS 2795-39-3 Remarks: Pusity 86.9%,Lot #217 METHOD MLTaehbtioshrosadtt/uogdruyyiwdPaeraslcitcnioecnefdo(ulGcltLoePwd)ediR:negcuolmaptliioannsce21wiCtFhRUnPiatsted58S,tawtietshFtohoedexacnedptDirounsg nAdomeidniosntrpaatgieon3(oFfDrAep)orGt.oodIt dpohcassenoctoampppleeatredtheaste tehxecep3tMionsEnwvoiurlodnmesingtnailficaLnatbloyraitmoprayctwtahse rpeseurlftosromredconicnlusaicocnosr.dancTeh.e awniatlhyti3caMl Environmental Technology and Safety Services Standard Operating Procedures. Test ype: invivo Speciesistrtayipeno/relienel: rauSprpareggnauntCerl--CDD(R)aBRVwAlFPelusy& Sex: FO:female,FI: both Age and body weight range ofanimals used: 65 days, 192-231 Nuofmanimbalsie sex/drose: FO: 8,E15 maleand female pups/iter Routeof administration: oral `Vehicle: 0.5% Tween(R) 80 in R.0. defonized water, dosage volume ml/kg Dboesgeisn:nin0g(4v3ehidcalyes),p0r.i1orantodc1o.h6abmigt/atkigon/dunaitynilvcoolnufmiermoedf$evmiidkegnc(e0.o0f0,ma0t.i0n2g,.anOdn0l.y32thmeg/FmOL)f,emoanlcees dwuelrye dosed. EFxOcurreitnie,onfercoeust,esse,rbuom,dyfivfelru.idsF,Ialnidvetriasnsduessemroumn.itored and/or sampledduring study: Statistical methods used: means, standard deviations, and percentages MRaetthdoamdsrweemraerekxsp:osed to PFOS via gavage prior 10 and during mating. Exposure to PFOS washaltedon itnheutfersrto adnady doufrpirnegsluamcteadtiogne.statMiaoln.e raPtusposfwtehreesanomtedsiorucrtcleyaenxdpotsreadintwoePrFeOuS,sdbuotlmya3yshbarveeedbeeresnacnxdpowseerde not administered the test article or considered partofthe test system. UinriitniaetiaonndoffeccaolhasbaimtpaltieosnwteorethceolfloelctleodwifnrgommoFrnOinfge,madlaeyrsas6 f1o0r 7t,he14fotloow1i5n,ganindter2v0alst:o o2n1eodfayprpersiuorme(d0 27 ENVAMRD(2002)1 FINAL gceosllteacttieodn f(rDoGm 6ea1c0h7o,ft1h4e10m1a5te,rnaanlda2t0s 0on2th1),daanydcdoahyabsitoaftlioancawtsoni(niDtLi)ate2d1(1p0r2i.oor Bcolhoaobditsataimopnl)e,sDwGer7e, c1u5llaenddt2o1,fiavendmaDlLe p1u4pasndan2d2.fivDeafye1moalfelapcutpastipoenrwlaiserd,efwihneerdeapsostshiebdlea.y oSferbaitsh.pecOinmenDsLw4e,rleecsosllewcetreed fasrsoimgnpeodolteodthfeisetrudsyamwpelreessoancriDfLice2d1., nOnald DiLver22s,paelclismuernviwvaisngcoGlelneecrtaetdifonroFmOeaacnhdaGneinmealr.atiTohneFlIivaenrifmraolms afeccahl,psueprwumasancodllleicvteredsaamnpdlpeosowleedrepsehilpitpeer.d toBltohoedSspaomnpsloersfwoerraenaclyoslilsc.tedOnanddaypsool1e1d0p4erofItterh.e 4U3r-idnaey, pSurbesmtaatnicnegipnevreihoidc,leF.O female rats received 25% greater dose due to an incorrect calculated amount of tet RESULTS Detailed results: Average Results for tDheGAnalysiDso7fSeraDSGaImSples (GP2F1OS ConDeL.sug/mi)D212 0000mmgekeeFFOI 0N.S1.00 00NS7% 00N7S42 <LNSLQ 00NS52 0N0SH 00N4S92 00..11 mmggkkgg FFIO 9N2S1 7N2S4 SN6s8 2N5S8 LNSe iNSs 0N9Sm 1166mmuekkeg FFIO N6S112N9S 9N0S6 3N9S5 2N0S6 2N1s 1N4S1 Average Results for tDhLe.Analysis ofLiver Samples (PFOS Conc. ug/g) 0000mmggkkggFFl0 0021473 00..11 mmgekgg FFlO 561050 1166 mmaerkkge FFIO 550672 A0v0emrgagkegRFeosults fo0r006th00e8A1n9alysis oofU0cr0ia1n07e0 Samples0D0(o0P6Ft8Ot5S Cone. uD0g0Gi0om6z)i14 "oLLaowge O16lmmgekggFFoO 0209105 00.5038087 000631237 000324301 00000353545 0Av0emreagke RFeosults for0tNh6e0DAnalysis ofFoeNceDers Samples (DNPFaDOsS Cone. uDg/Nga)Dz oNuD O1l6mmggkgFFoO 01.60091 0833999 042984 0210169 00035872 sItnheerguelnemoevreflaslt,hienthtdehraeemwssaesraunamd ddtehoecsrep-eurapessl.eadtewTdihteihnlcterivemeeal.sseoTifnhPetFhOelSelveevlweseolsrfeoPfsFiPmOiFSlOaSrweiirnnetthmheeulclhiivehvorifagetnhhedrersiednratumhmsofalinvdtehrpetuhdpasan,msiwnhaitnlhdee the levelsin the serum were slighty higher inthe pups thn in the dams. 268 ENVAMRD(2002)1 FINAL RAwiestshusoltutattseadRdeiimnoa-trlhakebse:rleepdortr.efe"rIetncies mnaotterpiaols.sibTlhee1o0nlvyermifeyasturrueemreenctoovefryacocfureancdyoagveanioluabsleanaalythties ftirmoem, tmiasstueis spike studies, indicate tha the data are quantitative to 50% or greater Metabolites measured: none CONCLUSOIONNS eee ee eed HL] USGetnnadetereramtetinhotenocfo0nCtdeoisntticoslnyusssotifeomnthae ipreasendtossteuddyw,itphertfhluotresotocstuabnsetsaunlcfeondautreinwgahs eobisne-rfveedphian saell osafmheplestutdyyp,esnodfifln all sample types oftheir offspring (Generation FI). Agree REFERENCE a USRretipunodery,t Taiitnllld::FeDcOeertsaelrom(fiGnCaavrtaiCgoenDo)BfPRthhaerVmACaFocn/ocPkelinuntesrtaitcioRRnoaetfcsoPvEeexrrpyfolsSuetouddrytoooofcPPtFFaOOnSScsviuailnfG(RaoPvanFasaOgtSeAe),nan3lyMtthiecEaSnlevriLuramob,nomrLeaintvtoearsly ILna-bLoirfaetoStruydRye4p1o8r.t0N1o5., MFaAyC4T,T20O0X1-.111, Laboratory Request No. U2094, 3M Ref: No. T-6295.14, Agus 269 ENVAMRDOO)I FINAL Tile: HAIFLIfe Study of PROS in Serum, 2000 TEST SUBSTANCE dentty: PFOS METHOD -- ol ed Study design: PFOS halflife study on rivedworkers fom the Datu, Alabama plant ManufacturingProcessingUse: N/a Hypothesis ested: To determine the halfofPEOS in eid pecfluorochenical production workers. Study period Nov. 1998 t0 2003 Setting: NA "Total population: 27 ees rom the Decatur, Alabama nd Cotisge Grove, Minnesota. plants Subject selection riteria: The participants volunteeredforthis study "Total # ofsubjects in study: 18 males Comparison population: N/A Participation rate: Uskaown aSvuebrjaegcetodfes2c8riypetairosn,:avReertaigreeeismreanfgreodmirneatgierefmrenotmt5o5s-7t4ryoefasrtsu,dwyorwkaesd3i0 mtheopslant for an (ange: 5130 months). Health efects studied: NA Data collection methods: Blood sera samples collected evry 6 months Details on data collection: No information was providedss to how the blood was drawn, stored, tc. Exposure perio Ukaown eDxepsocrsiuprteiogrno/udpesiinceaatteigoonrioefs: N/A Measured or estimated exposures NA Exposure levels: NA Statistical methods: Medians and ranges calculated. Oftohgeirnemaetrheoldaotiloongsihciaplwiansfuosrmeadt0iones:tiHmaatlefltihevssewreurme ucoarlouclhteendicaaslsuemliinmginaatoinoen-h<oamlpafriimnepnarmotdceilp.rinAg m ENVIMRD(2002)17FINAL t`rheeteqiurraeetelsi.aontisolnIonsphtieph=s2-lko,g(02i.n.3c06a3r)9.r3elOanticoensthhiep,ctlhimisnlaotpieoonfctohnsetafnitneisi carleclualtaetdedt,o tthhe healliFm-iinfaetiiosndectoensrtmainntedviuasitnhge r1e3tiredeatwaapsoninottsiwneclruedendotinavtahielaanballeysfeors.anEyigohfictehen spaurbtjieccitpsanatnsdmieftthheerseewraesquairleamckenotffsit {0 the model, that RESULTS etter eeeeet shDoeusclrdibbee rneostueldtt:hatThtehemdeidfifearnensceeriunmsheraumlPfFoOfSPlFevOeSlswbaestw2e7e0ndraeytsi,reewsitwhasrqaunigteloafrg1e3(90.t2o -642.00dpayps). It A``Smltetuahdsoyuurgsehtmretenhnitgstwhmasasyahnjiudgshtweierfaytkhnnaoentsstihenesc:lfuirdsiFt.nogrTtmhhoeosrsteefopoafrr,ttihhceieppaadrnatttisaciidnpiatdnhtesmoatnnoatflityistnihcsel,umditoeddiseilannatnhidendtainhcaaeltyyisowinse,roefthetexhcesleumdcaeondn.yd dalaistmsiaotcaiintaittoehndes rwoeifptohrttht.eheIdnasteaa.rdduimtIitPonFi,sOnSsoathiaenlddfilviiinfdeutahclealdcrauetlpaaotrwiteonrtseh;apthroonweveiivtdheeerdr,iantghtiehsensroteraptoenrmtuemnabtnedirsthnoeoftrmeslouanpttpiohornstsherideptwibrieettdhwweaeannyy tenhxuicsmlbouecdcreudrorfferdyo,emabrtushteestxehpecoaeslxeccdulluaisntiiotonhnesoiffwtothrhekoipsrleasrceeectoiarnneddsmiPenaFtsrOuoSdruelcmeeevsenlbtsiawasansdtohhtiaghlhfe-rriefsteuhlwsae.rtehAelesfxoicr,sl.uldiemIdi.nisatuRineotkninrooeewfsnPwFweOhrSye adocelctfueirrfsmeinvfieardotmuhraitnheeth baondhaydl.f-fleFicifeensa;slyuh,ogwtgehveeeersbf,ftyectetthhedoesfepcromeneltaiimsniuunreaedrmyneornents-suolctwesuerpreaeptoirnotonetadlt,haeklreoenw.-rleevpTehlrereeexsfpoeoarsneu,rtruseeitolnciamtnihnneoathtailobfn:e life is unknown. Research sponsors: 3M Environmental Lab `fCeooxlnplsloaiwnsaettdeinoocnvyienrotfahirsSes-cuuylretraser:ntpreTerhipoeodrs.teasrIens1u0lttwhsahtyarsettuhndeoyrt, ictohsnesieusstateicnlmtaarthwgeiedthdhiaftlfheferieffniercsetwiasntsuthd3eyr0ine4wshyiueocarlrhws.th3syrTenthieorreneeos fiwstehnreoe rettees in the current study didfallwithin the rangeofthe ist study. CONCLUSIONS NA REFERENCE ee eeee elL D1e3t7e5,rm8i(na)tSiuonpoplfeSmeenrtuaml HSaulbm-iLsisvieoson,fSSEeHvQe-r0a3l7l3u/o0r3o7c4h.emicas, June 8, 2000, 3M Company. FYLL0700- m ENVIMRDQOOITFNAL ACUTE TONICITY STUDIES Tie: An Act Inhalation Toc Study ofT-2306 CoC nthe Rat. 1979 TEST SUBSTANCE | entity: Potassium pefluarooctylaloate, CAS Nos 2195.39: Remarks: Dus, PROS (T-2306 CoC). Purity not specified. METeHODn. el Methodguideline followed: Similar 0 OECD 403 GLP: N.10 QUOC indicted Year studyperformed 1979 SoeciesStrain: RatSprague Dawley Sex (ialesfemales both: Bash No.of animalsscxdoses sx/group RouteofAdministration: Inalaon 41R0eGmiagurhtkcse:t0Cgaordnomcuipensni.isrtAeariWotrnhisegohPftRdOu1Ss.8t9,deu2se.t.d86mR4ea5tc5sh,awnei4rs9em,c7wx0ipt5oh,sed1dr3y90a,ir24|a.th0so9,rF.4o5w.T9ha7etemeogtf s1P2rO1oSw16terereasednmpiorninsmtpesrmeo.d r`29ro9eugeattxsp.sotusTdhyteonadttrasyiwioenr.raTohbsfeelrocovwnettdreoolrogfsro12unpHetorss Spwieegringmshipermdieo2r.0t3oA2el6x3opeissraeprdsottyo1col5ssmtnenmr.mthlTshasepsodoutlit ne hDI-aehyoru4,reeDsxapyfoosr7u,r1ae4,nadayDsar.eymTo1n4v.daildItfuaolmrbehpeooredtxphoyastuwrsewleecahriaemmrnbgeecsroh,rddtheyodiunrgolnyspDfuoorymaf0no(uepohotoryurosweaxreepmoxepseosbp,ys|ansdDeeis5h2.. eporsrsboirc apfrtorviddeeadth. Blood samples were collected on Day 14 ho 1 uv ams os RESULTS . UL . LC = 52.48 - 6.4) ml (95% confidence mit) eirenced method of Lichfield and Wilkosan Number of deathsa sch dos evel (by sex) 07.008mgm:g:0/110:0:12.8490m9emlaT:0101:120.06amuthlor: 1i1d0:m4o.8pmrovlide u210m6m.49bmyse)10, sDRrea0myuap2.rwkasAs: aalTtshoetpehirimgtihneoasttneyddoa5selayngirmo(auDlpas,ys4u15)r.vbi9evcemadugsa,endowfbaalsomonedoctshaaumnspielcdeaslinwpterhroebelLheCmesunrcaiatnurgtmeiinxoapntoissoena..ndTTthoereemoi1nsa5tiemdmgostn m ENVAMRD(002)17FINAL werealsonot used in the LC. determination. rmIeonsrptteahceltiit2vy4e.lw0yi.9thAmlgta/s2lt 8de6exapmtohgssu,ratedDegaartyohuspo10,c. caulArtrae4n.d5oi5mn,a2D8d6i,ae7daanybndyd1D.1a08.9y mA6/.tl1.tA8ht9erme7e.wl0a5,soa2nn0ed%d6,e.a41t90h%mo,gc/alcnudtrh0er%reoe dnmwoaDrstaaylit01y.%. do"itTshhteeurrrabtnasansciaenlsa,lldaitnshdcehsgaeernggeerr,oaulyppesolsolhorwocownemdadittesirioignan.lsofAarboonxuoinrdcmtaytlheiincnalnufodgienongbisteeamrlavcairtaeitgoiinoosnn,,werrderedyrmearptaoelrretsieadoarforoobutenhdeJrehssebrfneraeotqehuienongr in the lower exposure groups. to"rbTheseaetrmmvoeesndtt cienolamctomenodtnraolbanbonrroamrsamlaailntidytywtawhesarsevfadorirsyecionmlgoardyaetgirnoeonetsoobffetdhietsrcieoavlteomreanatntidorenfoluanftget.d.hDiTslhcieoverlreo.froarAteir,onnotohnfe tamhneoismhtaulnssgirgwnaaitsfidacilaesndot spmraelmlatiunrteelsyt,inedewcerreeasaeldsoboobdseyrvweedi.ght, discoloration of the lung, and discoloration and distention of the CONCLUSIONS - aLvCaSi0l=abe5.d2at(a 4- 64.4)me, (95% confidence limits). Only conclusion provided: seems reasonable with A REFERENCE rere eee tRhuesRact.h,PrG.oM.j,ecWt.EN.o.Ri7n8c-h7a1r8t5,anBdiCo.lAdy.nBaomziacks.In1c.979. An Acute Inhalation Tonicity StudyofT-2306 CoC in m ENVAMRD(2002)17FINAL "Title: Fluorad FluorochemicalSurfactant FC-95 AcuteOralToxicity (LD)Studyin Rats, 1975. A TEST SUBSTANCEee Identity: Potassium perfluorooctylsulfonate, CAS No.:2795-39-3 Remarks: FC.95, Purity not specified. METHOD - Method/guideline followed: Similar to OECD 401 GLP (VIN): N, 50 QAQC indicated ear study performed: 1975 `Species/Strain:RayCharlesRiver CD. Sex (Malesfemales/both): both Nuofmanimbals'e sex/drose: /sex/dose Vehicle: 20% acetonec/o5m0o%l Routeof Administration: gavage aRsemvaorlkusm:esLeovfel1s0omfil1k0g0,b2o1d5,y46we4i,ghatn.d 10T0h0emgra/tksgwPeFiOghSedwer1e72te2st1e2d. gAlaltdtohsee lbeevgeilnsnwienrgeoafdmithneistsetrueddy `iambmneodrimaatlelsiygnpsridourrtiongdotshienfgouarndhowuerisgahftesrweexrpeosruerceo,rdaenddadtaiDlayyth7eraenafderDfaoyr 141.4Tdahyes.rats wiserreepoarbtsecdrvtheadtfaollr danaiymsatlusddy.ying spontanwceoreugrsoslslyy nectopsiedas, well a al rats that survived t th end ofthe 14- RESULTS LDS0: 251 (199-318) mg/kg, 95% confidence limits)3: referencesforstatistical tables are given. N21u5mbuekreo:f d2/e5atmhalsesa,t e1aSchfdoesemleav4e6ll4(embyessge;:x):15/030mmalks,:5/50/f5emmaalleess., 0/5 females; 1000 mgke: 515 males, 515 females Raneimmaarlkssi:n thAell21r5atsmgi/nktghegr4o6u4p adniedd 1p0re0m0atmugre/lkyg. doIsteapgpreoaurpsssdiigendsotbeofxoirecitthye emnodstoffretqhueensttuldyy.absTchrrveeed isntcaliundeedd:urohgyepnoiataclivrieyg,iond,ecsrteoamsaecdhldiimsbtetnotnieo,n aanndd astiagxnias. congestion. No differences betweensexeswere noted. At necropsy of irtation of observations the glandular included: mucosa, yellow: and lung LLDDSS00 mfeamlaelreatrsa:ts:232371(1(6200-03-3396)9m)gm/kgk(9(59%5c%oncfoindfeindceencleimliitmsi)ts) m ENVAMRD(2002)17FINAL CONCLUSIONSEE None specified beyond LDSO eeeeee errata] REFERENCE eereetaen SDuerafna.ctaWn.tP.FC-D9C5.AcJuetsesupO.ralGT.oxTihcoimtpyso(nL,D)G.StRuodmyigi,n Raantsd. DS.tuPdowyeNllo.. 1193775-.083,FlIunotreamdatFilounaolroRcehsecmairccahl `7a8nd-1D4e3v3el,oBpimoesnetarCcohr,poIrnact)ion. (Includes Acute Oral Toxicity Study in Rats with T-2207 CoC. Project No. us ENVAM/RD(2002)17FINAL SKIN IRRITATION "Titles EyeandSkin Iriation Report onSample T-1117. 1974 TEST SUBSTANCE IRdeemnatirtkys:: PFoCt-a9s5si,uSmapmeprlfleuoTr-o1o1c1t7y.lsuPlfounatnreo,tCsipAeSctifNioye:d. 2795.39-3 METHOD. MeNottheopdHGuoiftdeesltinmeatfeorliallo:weNd:otNosptescpiefciiefdied Test Type: in vivo SSepxec(miaelseissftemaailnesittebyloptleh:):RaNbobtitsspaeclibfiinedo "NTuotmabledorsoe:fanaipmpaelsa/rssext/odbosee:1.06gtroatmal, 0.5 placed on each of2 prepared tes ites (inact-wet, abradedVehicle: Nonweet?)N;otottaslpedcoisfeiednot specified LGernagdtihngofsmcalee: tSeestpamrataetreiaslcoireisnfcoorntearcyttwhietmhaanfiomarlmiactelilo:n a7n2dherdema formation are summed. Reference source not provided. `Rreammar(k0.s5:g)Soifxtaeltbimnaoterraibablitwsahspaldatcheideonhaisr bclirppaeddfeorordmin-ttheawicrtebwaetctkspreapnadrefdlatnekst,siatneds, ftihveentceonvtehsreodfwointeh `egdaeuzmeapwatacsherse.corAdfetderac2c4orhdoiunrgs0a.n2ds7t2anhdoaurrdsiztehdesccaolvee.rings were removed and the degreeoferythema and RESULTS orRessuwletlsl:ingInadetecctaesde.s itis reported the primary skin iitation scores were 0; which indicates no reddening PRermiamraircys:irNroiitantdiiocnastcioorneo:fzreerloiability. No QAIQC. No effects reported. CONCLUSIONS : Ncooncilmuitsaitoinon. Inadequate information is presented in report o evaluate quality ofstudy and validity of REFERENCE -- JN. oAA.10B2i87e1s,eWmAaeRnFdiDeI.nLrs.titHaureriIsn.c. 1974. Eyeand Skin tation Reporton Sample T-1117, Project Ed ENVAMRD@O2)I FINAL EVE IRRITATION "Tite: Eye and Skin Iritation Report on Sample T-1117. 1974 TEST SUBSTANCE - lj RIdeemnatirtkys::PoTt-a1s1s1i7u.m pPeurrfiltuyonrootocstpyelcsiuflifeodnate, CAS No: 2795.39:3 METHOD MeNottheopdHGoufidteelstinmeatefroilallo:weNdo:tsNpoetcisfpieceidfied Test Type: in vivo SSpeexc(imeaslsersaifnemcaellelsitbyopteho)r:liNneo: RsapbebciiftieNdew Zesland White Nuofmanimbalsseex/drose: 6isingledose "Total dose: it{FaCp:p9e5a)rbsy0.13gMr)a,m,tot(aplrdootsoceolnosttastpesecitfhieedr0.1 ml or0.1gand T-1117 i reported obe. solid BLuetnagth|obftribmseertveasttimoatnearinadlait eiancchonstcaocrtinwgiathftarni"maanlycaeclc:umcuolualtdedbed7i2 hsoucrsh,orarresgidueeoftest material was flushed rom the eye." OSbcsoerrivnagtmieotnhpoedriouds:ed:1hrN,o2t4shpbe,ci4f8iehdr,o7r2rehfrerenced - "The reaction tothe test material ws read according 10 the scaleofscoring for damage (0 the come, rs, and the bulbar an palpebral conjunctivae..." hReemtaerstkssu:bsRtaabnbcietwsawserienstpillalcededininocnoelleayres,stohethoetyhecrouelydenwoatsrluebftthuenitrrecayteesd. aOsnecotnetnrtohl.ofa1 girraempo(r0t.e1d)thoatf ptahlpreebarcatliocnon1jutnhcetitveaset maatte1,ri2a4l,w4a5,s arneadd7a2gahionusrts asfctaeletorfeadtamemnatg.eTthoethsecacloemcernit,eriiraisaraendnottheprbeuslebnatredanodr Rreufsehreendcefd.romEtahcehetyei.me Ittheapepyeeasrswetrhea ssccoorreeds,waenryeamcacxuimmualaltead |dihsocuhraragendor24rehsoiudures aoffeersttrmeaattemreinatl twhaesn decreased over the rest ofth study. RESULTS Corrosive: no Irritationhrsscor8e.:00O;n2l4hyrtsot9a.l3s3c;or4e8shpr:ro3vid.e7d.23hr3: ze;ro Tool used 0 asses score: Not specified Descriptionoflesions: none. DReecmraeraksse:s wIenraedenqoutaetdeatde4scrhiptaionndaatnd2d4isscuvsasliuoensiwnerrepeorzte.ro.Scores appear reduced in all rabbits overtime. m ENVAM/RD(002)17FINAL CONCLUSIONS Oprnelsyenctoendcliunsrieopnorptrotoviedveadluiantsetquudayliitsytohfastteusdtysuabnsdtavnalciediistryaotfcoinnclgustiooeny.es. Inadequate information is: REFERENCE J4.10A28B71i,eWsAeRmFaenIndistDei.tLru.t HInacr.ris, 1974. Eye and SkinIrtation Report on Sample T-1117.Project No. us ENVIMRD(002)1 FINAL GENETIC TOXICITY STUDIES S"TiAtlLe:MOMNUETALGLEANMIICCIRTOYSEOVMAELUPLAATTIEONTOESFT,T-12907814 CoC IN THE AMES TEST SUBSTANCE Identity:Potassiumperflurooctylsulfonate, CAS#2795393 Remarks: T-2014 CoC, FC-95, Purity not specified. METHOD. . Method/Guideline followed: Ames, 1975 Test type: Reverse mutation Test system: Bacteria; Yeast Gen Yearstudy performeds 1977 `SSapcecchiaers/oSmtyrcaeisn/ceeerlelvtiyspiealeeeDlAline: Salmonella yphimuriun TAIO0, TALS), TAIS37, TAIS3S, TAOS, `Metabolic activation: 0.1 + 05 mi $9 homogenateofAroclor 1254 induced Sprague Dawley at iver C0.o1ncpegnptlraatt,io1n.s0 tpegsptleadt:e0,.100l.u0gplpalta,te1,.100p0g/pplpatle,e1,0S.00u0g/ppgl/aptlea,te10a0ctpigvpaltaedte, 500 ug/plate nonactivated: Statistical methods used: None SRienmglaerkpsla:teThpeerrecwoenrceentnroatsiiognniwfiacsantropurtionteo;co(l2)vatrheianteigaotn(i1sv).e Fcoonrtrtohle twiamse wthheensoltvheenttesDt MwSasO;dotnhee(p1o9s7i7t)ivae cmoentthraonlessulwfeorneatceh,osqeuinnoalcicnoerdhiyndgrotxoides,traniintobfelionugretneest,ed2-aanndthraacmtiinveat;ioanndco2nd-idtiimoenthyalnnditionscalumdiende. (t3h)y!a IliambiotreadtorryepbeealtiesvteuddythawtasthedroenweawsisthomsetaeivnidTenAcIe0o0fmboutthagweinitchitayndwiwtihtthhoiustseacitni.vaTtihoen dboesceasutseesttehdewitetshtoiuntg gactpivaltionwiwtehreac1t0i0vaptigo/np.latH,ow5e0v0erug,/palatree,viaenwd o10f00thgefpdlataatesahnodws500thaptpwlaes,or1i0g0in0alplypltheo,ughatndt2o00b0e `temsutteadgewniciet1y0rw0haisegwhitahnidn 1t0h0e onxoirmca1l0 vsahreidatainony olfitghheontasspaoys.siTblheemruetpaegaetnwiacsacitnivaidteyq:utahteerebewcearuesenothseigdnosseosf mtouteavgaelnuiacittryeisnuasnwyoerfethaes tfholelrowss:tradionsset-ersteesdp.onTshee otveestr i3tchonScaenctcrhaatrioomnyscweisthwalsowaelssto innecgraetiavsee. eq(u4)alcr0ite3riXa tihnecrseoalsveeenqtuaclon1t0ro3lXfobracTkAgIrSo3uSn,d TfoArITSA3L70OanadndTA2IxS.33SX. bDaocskeg-rroeusnpdonfosreToAvSerS3ancdonDcAe.ntrations with lowest nm ENVAMRD(002)1 FINAL B --E--S-------------------------------- Overall results: positive, negative, ambiguous: Negative aGnednowtiotxhiocutefafcetcitvsat(iuonnconfirmed, dose-response, equi--vwitoh/cwitaholut activation): Negative with Cytotoxic concentration: 1000 g/m both with and without activation Statistical results: No statistics performed. Remarks: None CONCLUSIONS aAusthonre'gsatciovneclounsiroenpseaatreteasctciunrgatbeutbubtecfaorustehewwhartonwgarseatsaoknes.n Tashemufteastgecnhiecmiitcyalinisthneegfaitrsitvetesntotwbaescawuistehiint normal variation ofthe assay. REFERENCE. `SLAimtetaosn Blionmetioc, Innc. eKenlsinglPtloanty,eTMe/asrty.lMFainndial2R0ce7p9o5rrt1.9oS7u5.bsmMittoeudm0t: 3eMaEvCagolmupataeinoyn,noSfaiiTn-t2P0ca1u4l,iCMoiCntinensytohtea ssiol 250 ENVAMRD(2002)17FINAL ATiStlSesAYSAWLIMTOHNEPFLOLSA, ~19E9S9CHERICHIA COLIMAMMALIAN-MICROSOME REVERSE MUTATION TEST SUBSTANCE Identity: Potassium perfluorooctylsulfonate, CAS#2795-39-3 Remarks: FC-95, T-6295, purity not specified METHOD. nl Method!Guideline followed: Ames eal, 1975; Green and Mariel, 1976; Maron and Ames, 1983 "Test ype: Reverse mutation Test system: Bacterial Gury Year study performed: 1999 ESpsecchieersi/cShtiraacionl/leWelPl2tyuprefAeel lin: Salmonella yphimuriu TAIS3S, TAI00, TA9S, TAIS3T Metabolic activation: 0.1 ml $9 liver homogenate from Aroclor 1254 induced Sprague-Dawley rats wwCegollnppcllaeatnee.t,raa3tn3id.o35n,su0g0tf0epsultgae/tdpe:lapSt.laettwey,gp/hp1il0ma0utreipuwpmil:thte3a3c,.ti3v3aw3tg3i/3opnlptagenp,dl0a1.t0e30,3p3g1wp,y0l/0ap0tla,wtge3,i3p31a.t0pe0e/papgnlpdtlea5,t,e01,0,0030.0p33eppgwlpeapltlaeatt,wei,3t.h13o03u.0t0 wEitchoalnd3w3i3thpoguptlaatctei,v1at0i0onugplate, 3333 pe/plate, 1,000 pgplat, 3.330 plate, and 5,000 plate both Statistical methods used: None cRoenmtarro:ksh:eTphoesrietiwveerceonntoroslisgnwiefirceansttapirnotaocnodladcetviivaattiioonns.co(n1d)iTtihoenrespweecrifeic3 aplnadteisncpleurdteedstbceonnzcoefnatlrpaytrieonnc,an2d cnointttrofolluowraesneD,M2S-Oa;min(2o)antthhersaoclevneen,t wsaosdiDuMmSaOz:id(e3,)tIh CR-as1s9a1y wansd n4o-nrietpreoaqtueid.nol(i4)neF-oNr-otxhiedet.estTahreticlveehtibclee cmoenasnidreerveedrtpaonstistipveeripnlastteraoivnserTtAhOaSto,fTtAhIe0a0pparnodprWiaPt2euvverhAic,lethcronetohla.d Ttohebeinatcrleeaasset haa2d-ftooldbeinaccrceoasmepainniheed nbydabedoastefraesstpaon3soeltdo iinnccrreeaassienignctohnecemnetarnatiroenvserotftanhsepetesptlaartteicolev.erFtohratsotrfaitnhs TapApLrSop3rSiaatnedveThAicIlSe3c7ontthoelr.e "The increasehad tobe accompanied by adose response to increasing concentrationsofthe test article RESULTS Overall results: positive, negative, ambiguous: Negative Genotoxic effects (unconfirmed, dose-response, equivocal-- with/without activation): POS was not 3 ENVAMRD(2002)17FINAL zenotoxic when testedeitherwith or without metabolic activation eCyyttoottooxxiicictycownacsenetvriadteinocne:dCbytyoatosxliicghittyrweaduscntiootnedinatthSe0b0ac0tegr/ipallaltaewnw,ithout metabolic activation. This Statistical results: Resuls were not evaluated statistically tRheemvaerhkiscl:eTchoentrreolwsereno tstspecific confounding factors. Mutation frequencies were within the ange of CONCLUSIONS Author's conclusions are that PROS is negative inthis assay. This is accurate REFERENCE PMeRcOcSh.i,CoM.vSa.nc1e99L9a.boSraaltmoornieelslaInc~. Es(cChoevraincchei)aVCioelnin/aM,amVimragliinaian-2M2i1c8r2osFoimnealReRveeprosret MCuotvaatnicoen ASstsuadyy wNio.th 20784.0-409. Submite0d:3M Corporate Toxicology St. Paul, Minnesota 55144-1000 m ENVAMRD(2002)17FINAL `PTRitOlSe:, C19H9R9OMOSOMAL ABERRATION IN HUMAN WHOLE BLOOD LYMPHOCYTES WITH TEST SUBSTANCE Identity: Potassium perfluorooctylsulfonate, CAS #2795-39-3 Remarks: FC-95, purity not specified. METHOD . Method Guideline followed: Galloway, 1994 "Test type: In vitro cytogenetics Test system: Human eels in culture cry Year study performed: 1999 `Species/Strain/eell-typeleel line: Human lymphocytes iMseotcaibtoicliaccida.ctivation: Aroclor 1254 induced rat liver $9 homogenate, 15.0 iL, plus NADP and gC/onmcle,nt2r9a9tiuongs t/e3s4tme9dp:lg12,,.5 u4g4/9mlp,g2m4.,93p9g/9mlu,g/4m9l7withmolu,t 9ac9t.iv3atuigo/n,ml,149pgm, 199 ug/ml, 249. 12:5 with iagct/imvla.ti2o4n.9 ug/l, 49.7 g/m, 99.3 ugh, 149 gin, 199 gi, 249 pgm, 349 wg, 449 g/m Statistical methods used:Cochran-Armitage tet for linear trend; Fisher's Exact Test eRaecmharrekpsl:icaTtheerweaswecroensindoerseigdniafnicianntdeppreontdoecnotl udneivti.atTihones.ne(g1a)tiEvaechconctornoclenftorrattihoennwoanascttevsatteidoninarsespalyicawtaes; w"aDsMSDOMaSt O10pullumsi,thwehi$c9hmwiaxs; tthheehipgohsietsitvceonccoennttrroaltiwoansumsidtoimnytchienesCt cfuolrtutrhese;nionntahcetiavcattiivoantioanssaasysaaynidt Ccuylctluorpehsowseprheameixdpeosfeodrtothcehaecmtiicvaaltifoonr a3sshaoyu.rsTahnedehacrovnecsetnetdra2t2iohnosurosfhctaec.hOpnoesihtiuvnedrceodntmroeltapwhearseestefstreod.m euasecdh;rempiltioctaitce ionfdtehxe wuasscaebvlalturaetaetdmebnyt caunlatluyrsesinagndthethenusmolbveerntofanmditoonteicdoceslelosftinheat ploesaisttiv1e0c0o0ntcroellswepreer scutlutduyres;uc(2h)atshethsioslwvehnetreorthtehree caheemniecgaaltiwvaesreDsMulStsOa;ft(e3r)3thhrouerswaisncnuobaftoilolnowwiutph ast2ud2yhdoounrehaalrvtehsotugihmien a rseeccoonmdmensdtueddy. w(i4t)hTahecotensttinaurtoiucsleewxopuosludrehaovfe b2e2enhocuornssifdoerretdheponsoitniavcetiivfattehderepohratidonbeoefn athesigansisfaiycanits tiensctreaarstei(cp<w0a.s01c)oninsitdheernedumnbeegarotifvceelbles cawuisteh tchherreomwoassomnaol saibgenrirfaitcianotnsinatcroenaeseofobmsoerrevecdonicnentthreatniuonmsb.erThoef cells with chromosomal aberrations at any concentration ested. x ENVIMRD(002)17FINAL Ey eeeee emer fected Overall results: positive, negative, ambiguous: Negative Gwietnhotaonxdiwcitefhfoeucttsac(tuivnactoionnf,irmed, dose-response, equi--vwitoh/cwitaholut activation): Negative both CFiyrsttoctyotxoitcoxciocncceonntcernattriaotnison2s9t9esutge/dmal weivtihdoeuntcemdebtyaboalriecduaccttiivoantiionnmaitnodti1c99ingde/xm.l with activation were the Statistical results: Negative. Rreesmtaerdkwsi:thMi12t.8otpigc/imnld,ex26w.a9sugr/emdlu,ce4d9.378%ug,/m8l%, 9195.%3,gi15n%,, 11429%,ug/1m9l%,,24249%i,m6i9,%2a9n9du9g2%,in14c9ulmturdes a2n9d9 4g4m9 ,gma!ndw3i4t9hopugt/malc.tivation. Aberrations were analysed from cultures tested 199 gm, 249pgml, 4Wit9h.m7eptaibno,li1c99acgt/imvalt,ion2,4m9iutogt/imcl,inadnedx w2a9s9 wrgeidmulc.edAbbyer1ra2t%i,on4s19%w,er7e1a%n,alaynsded53f%roimn ccuullttuurreess ttreesatteedd wwiitthh 9f9r.o3mgcu/ltmulres1t4r9eagtemd ,with192999ugp/gm/lm,l.and 299 pg/ml. Only 27 and 4 metaphases were availablefor analysis CONCLUSIONS nn sAtuethdo.r's conclusions re that PROS does nok case mutation in human lymphocytes; this is correct as REFERENCE MLaabroir,atoHr.ie1s99I9nc.. C(hCroovmaoncseo)maFlinaAlbeRrerpaotrti.onsCoivnanHcuemaStnudWyhoNol.:e B2l0o7o8d4-L0-y4m4p9h.ocSyutbemsitweidthto:PF3OSM.CoCropvoarnactee Toxicology, S. Paul Minnesota 55144-1000. EY ENVAMRD(2002)1 FINAL "Titles Unscheduled DNA Synthesis in Rat Lives Primary Cell Cultures with PFOS, 1999 TEST SUBSTANCE entity: Potassium perfluorooctylsulfonate, CAS #2795393 Remarks: T-6295, purity not specified. METHOD - i Method/Guideline followed: Williams, 1977; Williams, 1980; Butterworth etal. 1987 "Test type: Unscheduled DNA Synthesis in Mammalian Cells in Culture Test system: Primary cells in culture cue: ear study performed: 1999 Species/Strain/cel-typeleel line: Primary hepatocytes from Fischer 344 ratmale at. Metabolic activation: None `1C0opngcienmtir,at2i.o8npsg/tmeslt,ed5:pg15/mclo,n1c0en.t0rautgi/omnlsbaentdw2e5e.n00i.g0/2m5l,ucgh/omsleanndfor40e0va0kga/tmioln. bSasei0d.5upxuogn/,cly,toonicity. Statistical methods used: None iRnecmuabratkesd:foTrhe19r.e6-w2e0.r0ehnoour,sitghniefnictahntaspsraoytowcaols tdeervmiiatniaotnesd.an(1d)H-Trtihpylimciadtienceualtdudreeds toonthecocvuelrtsulriepssfowrer3e0 emmiunlusteisonafatnedr swohriecdh fthoercedlasyswaetr2e-fixCed,afderriewdhoivcehrtnhieghetm,ulcsoivoenrssliwpesrewedreevemloopuendt,edfixoendsainddess,taidniepdp.ed15i0n Sceolllvsenpterfdorosteheweasrsearyewadas(5D0MfSrOom;c(a3c)h tchoevreewrsalispn) oandfotlhleowm-euapnrneepteantucslteudayr;gr(a4i)ntchoupnotsidteitveermcionnetdr.ol (w2)asTh2eCAoAuFn:t (05)atfolreaasttr5eagtrmaeintn 0perbenuccolnesuisdearbeodvepostihteivceo,nchuerrreenmtsvtehibcelaencoinntcrroelasvealiune,thaenmdleoarnannetinncurcelaesaerignrahien `1u0m%bearboovfe ntuhcelpeerwcietnhtafgievesoerenmionrtehenevtehgircalienscosnutcrholtchualttutrhees.peTrhceentpoasgietiovfe tchoensteronluscalteisfiinedtebstotchulotfutrhesesies RESULTS Overall results: positive, negative, ambiguous: Negative Genotoxic effects (unconfirmed, dose-response, equivoca-l with/without activation): Negative CCeyltlotmooxripchocolnocgeyntwraastisouint:abElxecefsorsiavnealcyystiostoatxiacnidtybaetlaonwd25a.t0 u$0g./0mlu,g/ml; weak cytotoxicity at 25.0 ug/l. 285 ENVIMRDRO0F2IN)ALI Statistical results: Th results were not cvahated saistclly. Remarks: There were notest.specific confounding for. concLusions "Theauthor concludes that POS is negative in this assay. This i sccurte REFERENCE CLaibfoornaetoMr.iAe.s n1c9.99.ViUennsncah,edVuAled22D18N2AFSiynanlthReespiosrti.n Corporate Toxicology St. Paul, MN 55144-1000 RCaotvLanicve PSruidmyarNyosCell20Cu7l8t0ur0e4s7w.ithSuPbFmOiS,teCdo1v0an3cMe 256 ENVAMRD(2002)17FINAL "Titles MUTAGENICITY TEST ON T-6295 IN AN IN 1V0 MOUSE MICRONUCLEUS ASSAY, 1996. TEST SUBSTANCE Hdenity: Potassium perfluorooetylsulforate, CAS #2795.39-3 Remarks: T6295, FC-95, purity not specificd. METHOD MethodGuideline followed: Heddle, 1953 Test type: Micronucleus GL: ear study performed: 1996 Species/Strain: Mouse; CrlCD-1*(ICR)BR Sex: Males & Females No. animalysex/dose: Sisexdose `Vehicle if used): Deionized water Routeof administration: Oral Doses: 237.5 mek, 450 mgrkg, 950 mek Frequency of treatment: Single dose Statistical methods used: Analysisofvariance; Dunnet's cst oRfemdaorsiknsg:mTahleesr;e wweerieghntorsainggneififcoarntthperomtaolceosl dweavsiat2i0o.n9s.3(71.)0Angi;mafolrs fweemraele9swiet ewkassan2d3.|12da9y2o5l;da(2)sttahret ovreahlicdloesew;as(5)deailolntirzeeadtmweantt;gro(3u)psthweetreestslaamstpelde7d2atho2u4r,s;48(4a)ntdhe72teshtoumrast;er(i6a)ltwhaesveahidclmeiconntirosal stwa4essriHnegOld;e `tChoentprooslistiwveerecosntarmopllewdasat 8204 hmoyures onclyyc.loCponhtorsoplhagmrioduepsdicsosnsoilsvteeddion fSwamtearleasnadndad$mifneimsatleersedcabcyh. g(a7v)agNe.o aclniinmiaclasl. e(x9a)miMniactrioonnusclewierweermeadeev.al(u8a)teNdoinnethcerobposnieesmoarrtoothwerofgrtorsesateedxaamniinmaaltsi.onsFrewqeureencmyadoef PoCnEsthevsse N1C00E0Sewryatshrdoectyteersm.inAedpboysistcioverinwgasthjeundugmedbebryoafnPiCnEcreaansed NinCEmiScrionntuhceleoapttiecd fpioelldyschwrhoimleatsiccoreirnygthtrhoecyftiress jouvdegrleedvbeylasobssigenrivfeicdanitnrtehdeucvteihoincliencPoCntErIolNsCiEn eraittihoesrsienxeoitrheartsaenxyathaarnvyeshtartviemse.t tBimoen.e(m10a)rrTohwe tMo.xTi.cDit.y wwaass. hcihgohseesnt odnostehetebstaesdi.s of2 preliminary dose selection assays bothof which showed significant toxicity at he x ENVAMRD(002)17FINAL RESULTS Effect on mitotic index or PCE/NCE ratio by dose level and sex: PCENCE Ratio 2375 mek 24 hours:males0.57 +0.11;fema0.l52es 0.10 48 hours: males 0.45 +0.04; females 0.80 = 0.10 45072mhoeukres: males 039 + 0.11fem0a.4l2+0e.1s4. 24 hours:males 0.75 +0.1;females 0.59 +0.08 4% hours: males 0.71 + 0.0females 037 0.07 72 hours: males 0.29 + 0.06; females 0.40 0.12 950 mgrkg 24 hours: males 0.56 0.13; females 0.59 + 0.08 48 hours: males 0.54 + 0.08; females 0.44 0.11 72 hours: males0.17 + 0,05, females 0.17 0.05 Genotoxic effects unconfirmed, dose-response, equivocal): Negative S"TthaetiPstCicEalNrCeEsulrtast:o was reduced in 237.5 mg/kg males at 45 and 72 hours; in 450 mg/kg males at 72 hours feamnadleisn a4t5072mph/okugrs.feTmhaelreeswaats48nohsoautrisstacnadllyinsi9g5n0ifimcga/ntkgincmraelaesse aitn 4th8eannudmb7e2rohfoumriscraonnducilne9a5te0duPkCeE.s OofvemrPtCheEcoinntbrootlhs minalaensyatnrdeafmeemnaltegsraounpd.rTedhuecepodstihteivPeCcEon:tNroClEinradtuicoeidnafesmiaglniefsicoannltyiantc2re4ashoeurisn,the number (R1e)mAanrikmsa:ls were examined approximately 1-2 hours before sampling fo signsof toxicity and mortality. Afenmiamlaelssinintthhee925307.m5g/mkgg/kdogsgergoruopuprebmeagianneddyhienaglaibyoutthe2o2ughhoouurtsthafetrrteraetamtemnetnt.perAilosd.o (at2)22Bohtohurmsal2emsaalneds imnintuhtees9.50Atmagb/okugtd4o6sehogurrosupafwteenttreiattmoencton|vuflesimoanls fwrhoemntthheei4r5c0agmeg/wkagsdoopseengerdoubputanrdecmovoerreedmailneas afnedw ffermoamletshef9r5o0m mthge/k9g50domsekgroduopsewagsrofuopunwderdeeadf.ouAnldldseuardviavnidngatanaibmoaults7a1ppheoaurresdanfoerrmtarleaattmtehnatt, pooninet.ma(l3e) wNaoteorthceornscluimnpictailonsiwgnesrewenroet nreoptoerdteod. r(e6p)orTtheedr.e(w4)asBondoyinwceriegahste cinhatnhgeepserwceernet nooftmirecproorntuedc.le(a5t)edFoPoCdEsanadt any dose level testoreadt any time period sampled. CONCLUSIONS "aTchceuraauttehosrecsosnmcelundtes that PFOS is negative in the mouse bone marrow micronucleus assay. This is an REFERENCE _ IMnucr.i,(CHH.V)1,996V.ieMnuntaa,geVniirgciintiyaT2e2s1t82o.n TF-i6na2l95Reipnoratn,InCHViVvoSMtouudyseNoM.icr1o7n4u0c3l-e0u-s45A5s.saSy.ubCmoitrtneidngtoHa3zeMltSot.n Paul,Minnesota SS1.44-1000. EY ENVAMRD(2002)17FINAL "Tile: IN VITRO MICROBIOLOGICAL MUTAGENICITY ASSAYS OF 3M COMPANY. COMPOUNDS T-2247 CoC AND T-2248 CoC, 1978 TEST SUBSTANCE Ipdeernftliutoyr:oocTt-a2n2c4s7ulCfoonCa;teLi-n4w2a9t9e,raT5-022%4b8yCwoeCi;gh2t2s.o5l%utoiofan orfteahcetidoineptrhoaduncotloafmmeothnyilumansdalmeotfhyl Nme-tmheatchryyllpaetrefsluaonrdoo2c2i.a5n%cosufltfhoenapmyirdiodcitnhiaunmoclh-lboarsiedde gsahlutaorfyalna:mide, Remarks: T2247 CoC, T-2248 CoC, purty not specified MT... cr ------ec tesa M19e7t3hod Guideline followed: Amesetal, 1975; Zimmermann and Schwaier, 1967; Brusick and Mayer, "Test type: Reverse Mutation; Recombination Test system: Salmonella typhimurium; Saccharomyces cerevisiae Gen Year study performed: 1978 SSpaecccihcasr/oSmtryaciens/cceelrelvtiyspiealeeeDl3line Salmonella typhimurium TAIS3S, TA1S37, TAIS3S, TA9S, TAIO0; `Metabolic activation: 0.5 ml of 10% 59 iver homogenate fom Aroclor 1254 induced rats. `1C0o0n0cepnptrlatei,onSs0t0e0stpedg:plPaltatee incorporation assay: 10 g/plte, 0gplate,100 pg/plate, S00pgplae, Dessicator method: 0.1 midesicator. 0.5 midessicator, 1.0 mldessicator, 5.0 mldessicator `eYaeasstt rreepceoamtbiansastaiyona:t 10..01%%,,20..05%%,,41..00%%,,55..00%% Statistical methods used: None. cReermevairskisae: aTshsearyewweerree pneorfsoigrnmiefdicawnitthprbotootcholchdeemviiactailosns.and(1).wiTthheopnleatpelaitnecopreprorattesitoncoanscseanytraantdiont;hethS.e d"eTsAsi9c5ataonrdasTsAaIy0wDasfopretrhfeortemse.d HwoiwtehvTe-r,224g7ivCenoCtheuscionmgptlewxoitplyoatfesthpeer dceosnsciecnattroartaisosnabyuatnudsetdheonimliytsattriaoinnss iacntviovlavteidonincosnedtiitnigonitsuap,ndthiisnciluadcecdepstoadbilu;m2)avitdhee,po9s-iatmiivneocaocnrtirodlnse,we2r-enicrhoofsleunoraencecsondrd2t-ioanntthgherasmtiaiinne afnodr tdhiecppolxatyebuitnacnorepofroarttihoen aS.sscaeyr;ev1i,s1i-adeicahslsaoyr.oetThhyelenneegfoativtehecdoenstrioclcagtorrouapssfayowaillthasTs-a2y2s47waCsowCataern,d (13.)23T,h4epalsastaey iwnacsorrpuonraotniloynoanscsea.y(w4i)tFhobrotthheadgeesnitcscaatnordatshseaye,alsatteasswsaeyrewipthreT-p2a2r4ae8sdwfeorrethreepsetaantdeadrdthaessdaeysibcuctatnoor fweastscpheermfiocramledwabsotahdwdietdhtaontdhewiatgharo.utTmheetasbtoaliincsatcetsitveadtiwone.rePlS.tetsywpihtihmouurtiiudmsTwAerSe aplnadceTdAsi1d0e0.byThseideteisnt 2 ENVAMRD(2002)17FINAL awapserpfloarcaeteddisnhethleficnentaeOrolftanddesaitctcaacthoerd. toAthkenobwonttvoomlouftmeheofshTe-l2.24I7n dweacsreaasdidnegdotrodear,gl5a.s0smPle,tr1.d0imslh,t0h.a5t mpolsiatnidve0.c1onmtrloolfctheesmticchaelmwicaasl1w,e1r-deicahdldoerodetthoytlheened.esBicoctahtowr.erTehterenaetgeadtinvethceosnatmoel cmhaenmniecralaas Ts-2w2a4t7e.r;Tthhee dsteisriecreawtiotrhwvaasnesseawlaesd aplnadcepdlaicnetdhoenbaasemoafgnceatcihc dteismiecrcaptloartteoienns4urreooamdemqauiantteaidinsepderasti3o7noCf.thAe cmhaegmniectailc Pilnactuebsatwederaet 3i7ncCubaftoerdanfoarddi8tihoonuarls,42rheomuorvsebdefofrreomretvheertdaensticwceartersc,ounttheedi.r lids replaced and they were ANNAC AACA and nd nna Overall results: positive, negative, ambiguous: All estswere negative. wGietnhotaonxdiwcietfhfoeucttsac(tuinvcatoinofnirmed, dose-response, equivocal - with/without activation): Negative both CInyttohteopxliactecionnccoernptorraattiioonn:asTs-a2y2,4T7 w-as2nwo2atsc4y(toox8tioxict.o strain TALS3S at1000 plate and to al other stains autgpSl0a0t0epfloraatlleotwhheernsttreasitnesdwwhietnhotuetstaecdtiwviatthiomnetIabwoalsicoaxcitcivaatti1o0n.00 g/plat to sirsin TA1S37 and at S000 T-22458 was slightly toxic 0. cerevisiae D3 at % concentration without metabolic activation, Statistical results; No statistical results were determined. bReemsaormkess:liIgnhtthiendfiircsattaisosnaoyfwmiutthaTge-n2i2c4i8taynadtSt.hecehriegvhiessitaecoDn3cenwtirtahtoiuotnmteetsatebdo,li5c%a.ctTihveatiaosnsatyhewreassereepmeesdtetdo amtut1a%ge,ni2c%,dos4e%,resapnodns5e% ancdotnhceenttersattiingonlsabwoirtahtorayndconwciltuhoduetd tahcattivTat-i2o2n.48Tdhiedrenotwacasusneoreicndoimcbaitniaotnioonfina cerevisiae D3. There were no test-specific confounding factors in any aspect ofthe test, CONCLUSIONS Nii) "TTAhIe0t0e.stiTngAlIaSbo7r,atoTrAyIcSo3nSc,ludaenddtThAt9T8-22w4h7enandteTst-e2d24i8n waerpelantoenmiunctoargpeonriactiofonr Sa.sstayyphwiimtuhriaunmdTAwiItSh3oSu,t `imneatadbeosliiccaatcotrivaastisoany;ftohatvoTl2at2i4le7cdhiedminoctalinadnudcethmatutnaetiitohnericnhSe.mticyaplhiimnudruicuemd TreAcSoSmbainndatTioAn10in0S.whceernevtiessitaeed: "DT3h.ese conclusions are accurate, REFERENCE SCiOmMmoPnO, UVN.FD.S19T7-82.24I7N CVoITCRAONMDICTR-2O2B4I8OCLoOC.GISCRIALIntMeUrnTatAiGonEalN,IFCinIalTYReApoSrStAPYrSepOarFed3fMorC3OMMPANY. Company. St. Pau, Minnesota 55101. 20 ENVAMRDOOD)I FINAL REPEAT DOSE STUDIES "Title: First ninety-day thesus morikey toxicity study, 1979 TEST SUBSTANCE Identity: Potassium perfluorooctylsulfoCnAatSe#, 2798.39-3 Remarks: FC-95, punortsptecifyied. METHOD Method/guidelinefollowed: None Study duration: 90 days GLP (VIN): No Vearstudy performed: 1978 Species/strain: Rhesus monkey Sex: Males and females Numberof animals per dose group: 2/sex/group Routeofadministration: Gavage Doses tested and frequency: 0, 10, 30, 100, 300 mke/day Post-observation period: None Statistical methods used: None Rineimtiaartikosn:andDistthiellfeedmawlaetserwewiagsheudse2d.7f5o-r4t.h1e0 vkee.hiclTeheconmtoronlk.eyTshweermealoebssewreviegdhedadil3y.0f5o-r3.g8e0nekrgaactlisntiucdayl ursiinganlsysainsdwebroedycownediugchttesd waterthee rbeecogrdiend nowifeentkhlgy.studHye.maTthoelosgtiucdaylwaasndtecrlmiinnicaatledcahfetmeirs2t0rydaaynsaldyuseestoatnhde bdreaaitnh wofertehewmeoingkheedy.s. TAhtetnheycrroopisdypatrhaethhyeraotidivweerr,eadwreeinaglhse,dsapfete,fpiixattuiiotna.ry, Tkiisdsnueeyss,wteersetesproevasreirevsedanidn cbounftfreorledannedutarall t1r0at%edfogrrmoaulpisn wtheereeyeesxawmeirneedprmeiscerrovsecdopiincaRlulys:seald'refinxaalti,vea.ortTah,ebrfaoilnl,oweisnogphoargguasn,scfyreosm, glailvleb,laldudnegr,, shkeianr,t m(ewsietnhtecroirconalryympvhessneoldse);, rdeutordoepnhuarmy,ngielaeluml,yjmepjhunnuomd,e,cemcaumm,macrolyong,larnedc,tunme,rvKeidn(ewyist,h cmoursdc.le)pi,tusiptlaereyn,, sptaonmcraecahs,, tpreossetsaieo/vuatreersu,s,tbhyornoeidf,bopearmatahryrrooiwd,(stbhjyumnucst,iont)r,acshaelai,vatroynsgilla,ndt,onlguumeb,arursipnianrayl bladder and vagina. m ENVAMRD(2002)1 FINAL RESULTS . ~ NO(dA oseanEdeffL ect): None LOAEL (dose and effect): None Toxic responselefects by dose level: All ofthe monkeys in the trated groups died. Statistical results: None `Rmegm/akrg/kdsa:y gTrohuepmdoinedkebyestwienenthdeay3s003-m5,g/tkhge/mdoanykgeryosupindtiheed3b0emtgw/ekeng/ddaayysgr2o-4u,p tdhieedmobreitkweeyesn idnaytshe7-11000, tahnedgtrhoeumposnskheoywsedinstihmeil1a0r msgig/nksog/fdatyoxgirciotuypidniceldudbientgwdeeecnredaasyesd1a1c-ti2v0iotyf,weemsetsmiesntw.ithThseommeondkiaerytshefa,robmoadlyl ioffftehneinmgo,nkgeenyesrailn btohdey 1t0r0emabnlidng3,00twmityckhgin/gd,awyeaglroeuspss, hcaodnvaulyseilolnsowainsdh-pbrrooswtnatdoinsc.olAoranteicornoopfsty,hesevfievrearl; haidsrteonlaolgiccoretxeaxmiwnaastinoontesdhionwleldntroematiecdrgorsocuoppsi.cNloesiootnhs.e lCeosnigoensstwieonr,e hnoetmeod.rrhage and lipid depletion ofhe CONCLUSIONS Remarks: Authors conclusions sated above i results. Reviewer agrees. REFERENCE Gtooxlidceinttyhsatlu,dy1. ,StuD.dCy.NoJ.ess1u3p7,.0R8.7G., IGnetielmaatniodJna5l. RMeeslevairnc.h a1n9d79D.evNeilnoepym-ednatyCsourbpaocruatteiotnh,esMuastmtaownakne,yMI. m ENVIMRDQO0)I FINAL "Title: Secondninety-dayrhesusmonkey toxicity study, 1978 TEST SUBSTANCE entity: Potassium pesfluorooetylsulfonate, CAS # 2795-39-3 Remarks: FC, puriy not specified. METHOD Method/guideline followed: None Study duration: 90 days GLP (VIN): No earstudyperformed: 1975 Specicsistrain: Rhesus monkey Sex: Malesand females. Numberof animalsperdose group: 2/sexgroup Routeof administration: Gavage Doses tested and frequency: 0,05, 1.5, 4.5 mg/kg/day Post-observation period: None. `Sctaotmipsatriecadlbmyeatnhaoldyssisusoefd:variBaoncdey(woinse,-whaeymactlaoslsoigfiiccaatli,onb)i,oBcahretmilceatl' atentd aunridnatlhyesiaspparnodprioartgeantewsits swienrge Dunner' mliple comparison ables tojudge significanceofdifferences. Rineimtiaartikosn:andDistthiellfeedmwaalteesrwweiagshuesded2.f7o-r3.t7h5e kvge.hicTlheecomnotrnokle.ysThweermealoebssewreviegdhdeadil2y.5fo5r3g5e5nerkaglatclisntiucdaly suriignnaslyasnisdwbeordeycownediugchttesd waterthee breecgoirndiendgwoefetkhley.studHyemaantdoalfoegric3a0lanandd90cdlianyicsaolfcthreeamtimsetnrty. anAatlynseecsroapnsdy thtyheroihedapra,ratlhivyerro,idadwreenraelsw,eisgphleedena,fpeitfuiixtaatriyo,n.kiTdinsesyuse,s wteesrteepsorveasreirevsedainndbubfrfaeirnedwneeruteralwe1ig0h%edf.or|malTihne: wtheereeyeexsawmienreedprmeiscreorsvceodpiicnalRluys:seald'rsenafilxsa,tiavoe.rta,Thbaeinf,olelsoowipnhgagoursg,aneysesf,rogamllcbolnatdrdoelr,anhdeaarltl (twrietahtecdogrroonaurpys vneosdseelsr)e.trdoupohdaerynnugme,alilleyummp,hjneojduen,umm,acmemcaurmy, gcloalnodn,, nreercvteum(,wiktihdnmeuyssc,lef)i,vesrp,leuenng,,pasnkcirne,asm,epsreonstteartieculteyrmupsh, bthoynreoifdb,onpearamtahryrrooiwd,(stihbymjuunsc,titorna)c,heas,altiovnasriyl,gtloanngdu,e,luurmibnaarrysbpilnaadldecroradn,d vpiatguiintaa.ry, stomach, esteslovaries, RESULTS NOAEL (dose and effect): None. ENVAMRDQOO)I FINAL LinOacAtiEvLity(:dsolsieghtanrdedeufcfteicotn):in0s.e5mrgu/mkagl/kdaaliyn:e phSoofstphsattoaolsse, diarhea, anorexia, emesis, occasional decreases s"Tiogxnisc(raensorpeoxnisae,lefefmeecstiss,bybladcoksestloeovle,l:de4h.y5drmagtkiogn/)dasyign-if4i/c4anmtonrekdeuycstiodnicdinbseetrweuemncwhoeleekstser5o-l7,, cmlairnikceadl daitfrfoupsheyoipfisderdeopulseatlivoenolianrtcheelasd.renals, moderate diffuse atrophy ofpancreatic acinar cell, moderate diffuse p1h.5osmphga/tgaksiedaaycti--vitcylinaincdalsseirgunms p(ostoatsssituomols(,fedmialaersh)e,a)r,edruecdeudcesderbuomdychowleeisgthetr,olre(1d/u2cefdemsaleersu)m, arlekdaulciende i0.5norgmakniicgpdhaoysph-actlein(i1c1a2lfseimganlse,s)(.Sof stools, diarhea), sot stool, diarrhea, anorexia, emesis, occasional decreases in activity; light reduction in serum alkaline phosphatase. nStuamtbisetriocfalanriesmualtlss: The statistical results are presented, but should be viewed with caution due 10 the small 41.55 mmuukkgddaayy-~sigsniigfniicfainctaentducretdiuocntiionnseirnumsecrhoulmestaelrkoalline phosphatase activity and serum potassium (females) Ranedma7rokfs:theAlsltudmyo.nkBeeygsininnitnhge o4n.5tmhegkfigr/stdoarysgercoounpdddiaeydoofr twheerestsuadcyr,iftihceesde imnoxntkreeymsisexbheitbiwteeednswigenesko5f ghaasdtrdoeinctreesatsiendalacttraicvtittyoxaincditjyusitncplruidoirngoadneoaretxhias,hoemweesdism,arblkaecdk tsotosoelvaenred rdieghiyddirtya,ticoonn.vulAsllioonsf,thgeenmeornalkiezyesd sbtouddyy t1re0m2b7linkgganatd wpreoeskira5t.on.AftTehre3m0eadanysboodfy twreeiagthmtendte,crtehaerseedwfarsoma 3s.i4g4nifkigcaanttthreedbuectgiionnniinng soefrtuhme wcheorleestceormoplaarnadblae50am%ornegductthieoncoinntrsoelruamnadlktarleianteedphmoosnpkhaetyass.e actHiivsittoyl.ogAitc neexcarmoipnsya,timoenanshoorwgeadn wseeivgehrtals tOrneiatmmeanlte raenldatetdwolesfieomnas.lesAhlladthmeodmearlaeteanddifffuesmealaetsrohpahdy omfartkheedpadnicfrfeuasteicliepxidocdreipnleetcieolnlsiwnitthhedeadcrreenaaslesd cseerloussizaelvaenodlalroscseollfszchyamroacgteenrizgerdanbulyesd.ecrTeawsoedmeaellessiaznedanodnelofsesomfalecyhtaopdlamsomdiecragtraenudliefsf,use atrophy of the Astluldym,onthkeeymsoniknetyhse h1a.5dmdge/crkega/sdeadyagcrtiovuipty.surSviigvnesdoufngtialsttrhoeinetnedsotifnatlhetrsatcutdtyo.xicDiutyriwnegrethneoftiersdtowcecaeskioonfaltlhye dstuurdiyn.gatnhoeresxtiuad,y daenhdydirnactliuodnedorblgaecnkertaololb,oddiyarterheema,blmiungcowuesreinnottheed.stooAlltahnodubglhosotdaytissttiocaollastigtnhiefiecnadncoeftwhaes n29o3akchgiaetvetdh,e tehnedmoeftahnebsotdudyy,weaingdhtthoefmtehaenmbaloedsydwreoipghptedoftfrhoemf3e.m1a5leksgdartopthpeedbefgrionmni3n.g22okf th0e st2u.7d5ykt1.0 ONenceroopfstyherefveemaalleedsnohatdrevaetrmyenltorwelsaeterdumlescihoonsl.estrol and another had a reduction i inorganic phosphate. tAolnlicmiotnykweeyrseinnottheed0o.c5camsgi/oknga/lldyaydugrrionugptshuersvtiuvdeyd aunntdilitnhceluedneddodfitahtehesat,udys.oftSsitgonlss,ofsngaosrterxoiiantaesntdineamletsriasc.t Orceclaatseidonlaesliloyn,s.decreased activity was noted in three of the monkeys. Necropsy revealed no treatment CONCLUSIONS Remarks: Authors conclusions sated above in results. Reviewer agrees. EY ENVIMRDQOO) FINAL REFERENCE. Gtooxlidceinttyhsatlu,dy.E.LS,tuDd.yC.No.Jes1s3u7p-,09R2.,G.IntGeemialtiaonndal1R.5e.seMsethcrhinagn.d D1e9v7e8.loNpimneentty-Cdoaryporsautbiaocnu,teMarthteaswuasn,moMnI.key 5 ENVAMRD(2002)17FINAL "Title: Ninety day study in at, 1978. `TEST SUBSTANCE Identity: Potassium perfluorooctylsulfoCnAatSe#, 2795-393 Remarks: FC-95, punortspeicifiyed. METHOD. `Method/guidelne followed: None Study duration: 90 days GLP (VIN): No. ear study performed: 1978 Speciesistrain: CD rat Sex:Malesand females Numofbaneimarls per dose group: Ssex/group. Routeof administration: Dict DEqousievsalteenstte0d0a,nd2,f6r,eq18u,en6c0,y:2000,m30g,/1d00a, y300, 1000, 3000 ppm Post-observationperiod: None. `Sctaotmipsatriecadlbmyeatnhaoldyssisusoefdv:ariBaondcey(woinse,-whaeymactlaoslsoigfiiccaatli,onb)i,ocBhaemricea'l atensdt aunridnatlhyesiaspparnodprioartgeantewsits uwseinrge. Dunnett's multiple comparison tables tojudge significanceofdifferences. aRneimmaarlkss:werTeheobmsaelrevsedwediagihleyd f1or9g6en-egr2ala3nc2dlintihcealfesmiaglness awnedighbeoddy16we5ig-h2tgs0a6wersetudryecionritdieadtiown.eekTlhy.e aHnedmaatfotleorg3i0caalnadnd90cldianiycsalocfhtermeiasttmernyt.analAytsensecarnodpusryintahleyshiesatw,erlevceo,ndaudcrteneadlsa,t tshpelebeeng,ipnintiunitgaoryft,hkeidsnteuydsy, ptersetseesrlvoevdariinesbaunffdebrreadinnewuterrael w1e0ig%hefdo.rmaTlhine;tthhyeroeiydejspawreatrheyrporiedsewrevreed wieniRguhsesdella'fserfifxiaxtaitvieo.n. TThiessfuoelslowweirneg oesrogpahnasgufs,romeyecso,ntgraolllbalnadddear,l hreeasrtte(dwigtrhoucposronwaerryeveesxsaelmsi)n,edduomdicernousmco,pilcaeluly,: jeajdruennuaml,s,ceacoruam,, cborlaoinn,, rneecrtvuem,(wKiitdhnemyuss,cllei)v,er,pluenegn,, spkiann,crmeeasse,ntperorsitcatlelyumtperhusn,odbeo,nree/tbroonpehamryanrgreoawl lymph node, mammary (sb junction), salivary land. gland, ulruimnbaarry bspliandadlercoarndd, vpiatguiintaa.ry, stomach, testes/ovaries, thyroid, parathyroid, thymus, traches, tonsil, tongue, 26 ENVAMRD(2002)17FINAL RESULTS NOAEL (dose and effect): None LliOveAr wEeLig(htdso.se and effect:30ppm (2 mg/kg/day)basedona significant increase in relative and absolute "aTtso.xidcierdesbpeotnwseeleenffdcatyssb8y-1d3o.s3e0l0eveplp:m3--00S0/Sppmmal--e 1r0a/s10diaetdsbdeitewdebeentwdaeyesn d1a3y.s257;-55./510f0e0maplpemra--ts10d/i1e0d bmeatrwreoewn) daanydsse15c-o2n5d.arAyt(s3p0l0e,en,10m0e0seantnedri3c00l0ymppphmno--dehsi)stlolyomgpihcoildesioorngsanisn, tshteomparcihm,ariynte(stthinyemsu,s,mubsocnlee amnedanskinb.od1y00wepipghmts-- 2w/e5remalreesduacnedd 2by f1e6m.a7le%s(dmiadlesd)urianngdw1ee6k3%5an(fdemaaltehsi)rdamtalsetuddiyedtedrumriinnagtiwoen,ekfo1o1d, ceomnosgulmopbtiinon(msailgneisf&icanftelmyalree)d,uceeudk,oesyitgneif(imcaalnets)r,eaduncdtiroenticiunlohceytmeat(ofcermiatles()macloeusn)t,s,ersyitghnriofcicyatnet (imncarlesa)s.e i1n00ab0saonludte30(0f0empalesp-) salnmidghrtetlaotimvaer(kmeadlefsoc&alfnemealec)olrfihveeorpawsteoicgiyhttesas.nd relative kidney weight. At 100, 300, l3i0veprpwmeig-hStig(nfiefmiaclaenst).reduction in food consumption (males), significant increase in absolute and relative AmiCdazlolnadlosreeglieovnesl,s -- very sight to sight especialy in males. cytoplasmic hypertcophy of hepatocytes in the centrilobular ro Statistical results: 100 ppm ~significant reduction in food consumption 2R5emaanrdkdsa:ysAl1l8o-f28thfeorratthseinmatlhees30a0n,d 1f0e0ma0leasn,dr3e0sp0e0ctpipvemlyg.roinuptshedi3ed0.0 pDpeamthgroocucpu.rreAdt b1e0t0w0eepnpmd,aydsea1t3h`aorcocuuprsresdhboewtewdeesnigdnasoysf8t-o1x4i,ciatnydiantcl3u0d0i0ngppemma,citahteirona,scdoinevdublesitownesenfodlalyoswi7n-g8ohfantdrleiantgm,enhtu.ncThheed braacsk,inrleld smaitmeurila,lraerdouucnedd atchteiveiyteysa,nydemllooiwsmraetdermiaatlerriaoluanrdoutnhde tahneogmeonuittahlorrengoisoen., increased sensitivity to extemal "aTnhdretehematlweos faenmdaltewsodfieemdadluersiinngtwheee1k0$0 panpdmtghreotuhpirddiemdalperidoirtedosdcuhriendgulweedeksac1r1ifoicfe.theTwstoudoy.f tAhet msatluedsy treesrpmeicntaitvieolny,. meAavnerbaogdeyfowoedighctosnswuemrpetiroendudcuerdinbgyth1e6.en7t%iraendswd1y6.3p%eriiondth(egrmataildeaya)ndwafsemsailgenifgircoaunptsl,y rceodmupcaerdabfloerammaolnegs tahnedcofnetmraolleasndat101000pppmpmg.roupAsf.terCl3in0icdaalycsheomfisttrreyatamneanlty,sehsematatoonleogmiocntvhalsuhesowweedre scirgenaitfiinciannetpihnocsrpehaoskeiniansemeaannd gallkuacloisnee ipnhomsaplheast,asbelovaoldueusrefaornfietmraolgeesn. vaAlfuteesr 9in0 mdaaylsesofantdreaftemmeanltesa,t a1n0d0 pcpomu.ntt:hehtewtohseuervsiuvrivnigvimnaglefsemhaaldessihgnaidfisciagnntilfyicraendtulycerdederuyctehdrohceymteo,glhoebmionglaonbdinr,ethiecmualtooccytreitcoaunndtsl,euaksocwyetlel SalsigShltigthotlmyodleorwaetreeirnyctrheraosceystei,n hpelmaastmoacrgihtiaamndicloexuaklocaycteetccoaunndts.pyrTuvwiootrfantshaemisnuarsveivaicntigviftieemsa.leUsrisnhaolywseids sSiegnsifsicwanetrley cionmcpreaarssebdleinatmheonmgaltersataendd aanbsdoclountteroalndgreoluaptsival3iv0eranwdei9gh0tdsaywse.reRseilgantiifviecanlitvleyriwnecirgehastedwaisn the females. Relative kidney weights were significantly increased in both sexes. Awlelreatrsediunctehdeb3y08p.p7mangdro8u%p sinurtvhievmedaluensiantdhefeemnadolef,trheespsteucdtyi.velAy.t sAtvuedryatgeermfionoadticoonn,smuemaptnibonoddyuwreiinghtthse 27 ENVAMRDQOODITFINAL ewenreecsotmupdayrapbelreioadm(ognrgattdhaeyc)onwtaroslsaingdnif3i0capntplmy grreoduupceadt f3o0r atnhed m9a0ldeasysa.t 3O0nepymf.e_malHeemsahtoowleodgicslviaglhuleys c9l0evadtaeyds,glouncoesemlaelveel asnhdowoende mmaoldeersahtoewedincsrleiagshetslyinincgrleaacsoesde,alkballoionde phuroesaphantiatsreogeenveanatd30y-dagyhs.uamAlt rmaalnesspehpatddassiegnaicftiicvaintty.deTchreeafseemsaliensahbasdolsuitgeniafincdanrteilnicvreeasaedsriennaablsowleuigthetasn,dabrseollautitvee tlhivyerroiwdepiaghrtast.hyrTohied u`wnecilgehatrasnidncaebssiomliluatrpchtaunagreys wwerieghntotTnhoetbeiodnlohgiigchaelrsdiogsniefgircoaunpcse.of he changes in male organ Weighs is Adetgrneeecsroopfsyd,isctorleoartamteinotn ealnadtoerdegnrloasrsgelmeesnitonosftwheeeivpersesaenntd diinscaolllortarteiaotendogfrtohuepgslaannddulairncmluudceodsavaorfyitnhge Sctyotmoapclha.smicHihstyopleorgtircopehxyamoifnahteipoantocalystoesshanodwefdocallesinoencsroisnisalwlavseaotbesdergvreodupns. thCe elnvter:ilthoebuinltcoridmeindczeonaanld pTeolmatvseouspesve,riutcyawtemreentarrealtatredinhtihsteolmoagliecs.lesIinonasdwdeorne,noestpeedciialltyheamproinmgarayts(tihnymtuhse,30bo0ne10m0a0rraondw)3a0n0d0 {Seycmounsd,arythi(ssplceoenns,ismteesdenotferdiecplleytmiopnh ninodtehe) nluymmpbheoridanodrgasnizse, sotfohmaechl,ynmtpehsoiindgsf,omlucsecsleaanndd isnkint.heTbnotnhee smiazreraonwdbnyupmocbeelrluolfryimtyphweasidnoftoeldl.iclTesheansdplceeelnlwaansdslisgihmtiylaar dveoplietdionwiwtahscnoorteesdpionntdheinmgesdeenctreeraisceliynmtphhe Hnoedmeso.rrhaMguecowsearle nhyopteerdkeirnattohseissaanndduolra pacoarnitohnosiosf twhasstoobmsaechr.vedDeincretahesefaotrreospthoymaicnhheandh.eimguhctosaanld daxhiecpkindeesrmoaflthhyepevriklelrwateoesisnaoendodnatchaentshmoaslilsiwnatssinne.dAitnrtohpehsyino.fthe skletal muscle was ted, as well coNcLUSIONS Remarks: Authors conclusions stated sbov in results, Reviewer ages. REFERENCE GStouldydNeon. ,13E7L.-08D5.,C.InJeemsastuipo,nRa.lG.ReGsiealrachndanJd.DeMsicnlogp.men1t97C8.orNprianttiyon-,dyMastutbaawcaunt,erMait.oxiiy study. 205 ENVIM/RD(2002)1 FINAL "iTnilCey:no4m-oWlegeuksCMaoprsiukleyes.ToxUincaiutdyiStteuddDyrwaift,h1P9e9r5f.luaroctane Sulfonic Acid Potassium Salt (PFOS: T-6298) ITpEsST SpUSBTSTAANNCCEE Identity: Potassium Perflurooctylsulfonate, CAS No.: 2195-39-3 Remarks: T-6295, purity not specifid. METHOD Methodguideline followed: Range findin-g Unaudited Draft report, sections missing Study duration: 28 days GmiLssPin(gVIN): Y, report contains GLP statement, but a submitted, unaudited draft report with sections ear study performed: 1995 `Species/Strain: MonkeyCynomolgus Sex: both Number of animals per dose: p2epresresxe,x,200mmgg/kkgg//ddaayy; 3persex, 0.02 mg/kg/day: Routeof administration: Capsule in stomach Doses tested and frequency: 0 mg/kg/day; 0.02 mg/kg/day2;.0mglkg/day Postobservation period: none Statistical methods used: none rReecmoarrdekds:twiMcoenkweeeyksly.wereHeombasteorlvoegdicaatl laansdt cdlaiinliycalforchegemniesrtarly calninailcyaslessiwgensreancdondbuocdtyedweoinghtssamwpelrees ccsooalnmlcpeelcnettserdaftobireofncolsirnweiectrahleectbhaeekgmeiinnsntoirnnygdwaeoyrfset+ch7oe,l2ls,etc3ut,deyd7,aotn14ds,atyaund7dy d29a(.ybsaIs2en,laid7n,deiatvniadolnu1,e4s.s)aaBmlnpoldoedsdasfyarmo2p9m.ledsaAyfdodr-i7tsieaornnudamldPabylFoO2oS9d wtheyrreoxiann.alyTzheed sftourdlyevaenlismoaflesstwreardeiotle,rmeisntraotneed, asstsicohe,dutlheydroaitd3s0tidmauylsa.tinAgt hnoercmroonep,sayrisoadmoptlheyroofniivneer, waansd cpoalnlcercetaesd wferroem ceoalclhecatneidmaflorfporrolpiafiemriattiooynl cCeollAnuocxliedaarseanatcitgiveintyevaanlaulaytsieosn.. SAamspalmepsleofoTfvefri,vertewstaess, aalnsdo rcoelalteecdteadrofurposmweearceheaxnaimmianlefdormiPcrFoOscSopciocnaclelnyt:raatdiroennaalnsa,lyesyies,. KiTdhneey,foilvlrow,ilnugngo,rgsapnlseefn,ropmanccornetarso,lfaenmodraalll bone marrow, testes, and thymus. 20 ENVIMRDQOO)I FINAL e RESULTS e ------------ NO(dA oseaE ndeffLct): None determined. LOAEL (dose and effet): None determined STtoaxtiisctircael rsespulotsn: sNoenlbe,yedhfoigsehe-cldeotvsesel0:0Nofneewdaenteirmmailn(es1dmale, 1 female Summary Hormone Analyses Data in Males on Females on Days 7 and 29 Toe| Se Foro TH Twine | Thm [[C Co oos rs[77TTossoteet0a0[|T m1l7amR r0r[|C o0vE 0b0u:oN 0o000o0E||T7EraTsra7e00||MioTmnnsipSeTsnTests ||ES4maiSostsi]| [[E FCo o[hhieesT[3H9TsewasieooT h|wan rere1r[C ooS obiwoooT1T 3o0-o05||iewwI soesmt--onT1ioso] ers|| [55C0R2 er I EE Femles [7Tab1202 24|7 P 5040s1l 0857|CC 0C0100B 00 |E2551E331E23056 |E TTotRbT osorns--E|"RE3EeTtEn] [E E ouF r r [E3A0N[snr7J a5nt| svv:0S90N1[ooCboToo0{E 21J4|Tnse7si3saSn sos oi | | CONCLUSIONS otEhsneterhafdieigmohla,dloeesseitnraotnnhie,mahtlhisygrhao-idhdosesetnigmdruoluaoptf)itnhagenhdsoturbdamyso.enleiSni(eTnlSeeHv)teh,lestnahruyermobvseairrni,saboalfntede,sirtieoddonatnohiymcralloesnairanrieef hlseemvaselellsh(wooernremeomnoaewleeJrvncdi]n changes are treatment elated. Remarks: None REFERENCE C(`ToPhvOoaSmnf:coerTd-L,6ab2PoJ0r.5a)t1o9ir9n5iC.eys4nIn-oc.Wm.eoMleagkduCisaspMosonun,lkeeWyIT.so.nicUintayudSittueddy Dwriatfht.PSeftluudoyrNooc.tTa-n6e2S9u5l.6f,onfiorA3cMi,d P.otaPsasuliuMmNSa,ltby 00 ENVIMRD(2002)17FINAL "TT6i2e9:5)2i6n-CWyeneokmCoalpgsuusleMoTnoxkiecyist,y 2St0u0d2y with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; TEST SUBSTANCE Identity: Potassium perfluorooctylsulfonate, CAS#2795.39-3 Remarks: FC-95, T6295, purty not specified METHOD Method/guideline followed: No guideline followed. Study duration: 78 weeks, 26-week treatment period, followed by 52 weeks recovery. GLP (VIN:Y `Vearstudy performed: 2000 Species/strain: Cynomolgus monkeys Sex: Males and females Number of animals per dose group: 6 aanniimmaallss/sseexx/dfoosegrgoruopup2.fo groups 1,3, and 4; Route of administration: Oral capsule Doses tested and frequency: 0mglkg/day,0.03 mg/kg/day, 0.15 mykgidaoyr, 0.75 mg/kg/day Postobservation period: 52 weeks SAtNatCisOtVicAal,mceotvharoidtse-uasdedj:ustLeedvemneea'nss,te5s%fotrwvoa-rtiialnecdephroomboagbeilnietiytyle:velA.NOVA, Duets test oRnecmearkdsai:lyAnfiormalasbnworemraeliotbiseesrvaenddtwsiicgensdaiolfy ftooxricmiotryt:aliftoyodandcomnosruimbptuinodniywaans.d waesrseesseexdamqiunaelidtaattivleelays.t `wOepihgthhtaldmatiac weexraeminreactoirodnesdwweereekldyonbeefboerfeorteheisntiatritatoifontroefattmreenatt,meonntDaanydsd--u1rianngdwe| eaknsd2w6eeaknldy 5t2h.ereBaofedry tBhleoostdaratnodfutrrienaetsmeanmtplaensdwaetrsepeccoilflieecdteidntfeorrvacllsinidcuarlinhgemtarteoaltomgeyn,t calnidnicraelcocvheermyi.stBrly,ooadndsaumrpilnealsyswiesrbeefaolrseo tStaukdeyn afnord hseonrtmotone3Mdetfeorrmiannaatliyosniss.of SPaFmOpSleslevoelfs.serTuhme wfeorlleowcionlgleoctregdanast wvearreiowuseitgihmeedpaotinstcshedduurliendgahned u(2n),schlievdeur,leodvasracyri(f2i)c,esp;apnacirreeads,ortegsatniss (w2e)r,eawnedigthhyerdoisdep(a2r)atewliyt:h apdarreantahlyro(2i)d,. bTrahne,feoplildoiwdiynmgisti(s2s)u,eskiwdenreey ceoslolpehcatgeudsf,oreyheisst(o2p)a,thfoelomguyr: wiatdhrebnoanlse (m2a),rroorwa,,gbarlalibnl,adcdeerc,umh,eacre,rviixl,eucmo,lojne,judnuuomd,enkuind,neeypsid(2i)d,ymliessio(n2)s,, lsiavleirv,arlyunggl,anmda(mmamnadriybulgalran(d2,),messceinattiecrince.vel,ymspehminnaoldev,esoivclaery(2)(,2)s,keplaentcarlemauss,clpietu(itthairgyh,),psrkoisnt,atsep,inraelctcuomr,d (wcietrhvipaalr,atthhyorroaicdi,c,tarancdhelau,mbuarri)n,arsyplbleaedndesrt,eruntuermusw,ithanbdonveagmianar.rowL,isvteomsapcehc,imteesntsis f(2r)o,mtthhyem0u.s,15tshynrdoid0.(725) 0 ENVIMRD(2002)17FINAL mg/day recovery annals were collected via biopsy and analyzed for PFOS levels, tSheerpurmesaenndceliovferPFsOpSe.cimSeenrsumcowllaesctheadrvfersotmedtefsrtoamnbilmoaolds twheartewasesntceontrtihfeug3eMd wLiatbhionraotnoeryhoaunrdofancaolllyezcetdiofnor SLeitvetro smpaeinctiamiennsspweecriemeflnasshatfr-o6z0entoin8l0iquCidunnitirogsehni.pBpoedthtolitvheera3nMdLsaeb.rumSasmapmlpelseswewreereshsitpopreedd ifnrozfeencasnedr oconvderryedictehefrino-mliCfeovpahnasceeoLfabtohreatsotrudiye.s Oton3eMrepecreiiovdeidcaaltly3MfrosmpeAcuigmuesnts w1e9r9e8 stthorroeudghinMfarrecehze20at00eitwhheirch55C +10:20Cor -20C + 10C. cDoulrliecntgedt.hOeFftihrest s2e6ruwmeeskpsecoifmetnhse, s1t5u1dywearetoftarlomofGr5o5u0ps1e,r9u9m fsrpoemciGmreonuspa2n,d15320flriovmerGsrpoeucpim3enasndwe14r8e ffrroomm GGrroouupp 44..EIinghtthelirveercosvaemrpyleGsrowueprse,c7o2llescetreudmfraonmd G4roliuvper1,sampflreosm Gwreroeupco2l,le1c2tefdofmroGmroGurpou3pan1d5 7124 Serum an4dliversamples from Grou3pand 80serum an4d lver samfpromlGreousp 4. LLiivveerr asnadmpsleersuwmesraemphloemsogweenriezeedxtrparcitored0useixntgraacntifono.npSaaimrpilnereexatgreancttsawnedrmeetahnyall-yzeerdtbuustiynlgchtihgehr-p(rMeBsEs)ur,e lrieqsupiodnse_cmohdreo.maPtRoOgSrlaepvehlys.w-eercetqruotsaapnnrdeatmyibytmeaaxststeemsadplecsttraonmdeatrrdycal(ibHrPatLioCn-. ESMSMS)in the. multiple LwievreersspaimkpeldewsitwheTreHPhFomOoSgeannidzeexdtrianctweadteurs.inAgnanalfioqnu-optaiorfinegacehxtrlaicvteirohnopmroogceednuartee. aAnndfaolnl-spacirruimngsraemapgleenst wdraysoanddaendi1t0rotgheenseavmapploersataonrdatnhdetihoennpwaierrsewreerceonpsatrittiuttieodnend i1n.t0o MmILBoEf.meTthheaenxotlracatnsdwpearseseedvatphorroautgehdaun0t.i2l jm nylon filer "cThhaeraactnearliyssteisc woefrtehepelrufoorromcehdembiycamlonoiftoirnitnergesotneusoirngmoHrPeLCp/roEdSu/cMtSi/oMnSs.selMeoclteedcuflraormfaonsi4n9g9l,e hpreimparriymafroyn qfuoanntfiortatPiFvOeSan(aCliyFsye5S0;) analysis, was fragmented to produce on 99 (FSO). lon 99 was monitored for RESULTS NOAEL (dose and effect)0:.15 mg/kg/day. LOAEL (dose and effec): 0.75 mg/kg/day. Death, liver effects, effect on cholesterol "bToodxyicwreeisgphotnpseerlceefnftcatgsesb,ylidvoesre10lebverla:inDweeaitghhtatr0a.ti7o5s mign/fkegm/adlaeys:aitn0c.r7e5asemdg/akbgs/odlauyt;e laibvseorluwteeigahntd, rleilvaetrivteo hJiivgehrdweenisigthyt;liMpaolperosteainndcfheomlaelstersolin,ltihveer0o7r5gamng/wkeigg/hdtasy. dose-group had lower total cholesterol and Tower Smtga/tkigst/idcaayl rfeesmuallte:rTeshtemedniftfegrreonucpesinwawseisgthattisatticatlhley esnigdnioffictanrte;attmehntefbfeecttweoenntotthael ccohnotlreosltearonld atnhde 0h.i7g5h 0d.e7ns5itmyylkigplopdraoyteidnosceholgersoteurpolthien tlhiever0.o7r5gamng/kwge/igdhotsse garnodupthweaosrgstaant-itsoticbaloldyysiwgeniifgihctantp;ericnemntaalgeessinwetrhee osrtagtains-tibcraalilyn wseigingihfticraanttoawnedreinal fesmiagnliefsicatnhte. liver weights, the organ-to-body weight percentage and the 0 ENVAMRD(2002)17FINAL Remarks w`tMreaeelaketssmoewnfetitggrrheoeaudtpms3e.n3tr-e,3sp.me4akctlgievsaelnywd.eifgFehememadallee3s.s7w,weei3.ig8gh,heed3d.52,.38.a-12,n.d93.3k1,g3 a3k.1ethafenordbetg2h.ie8nn0ki,en0go.f0of3rt,thh0ee.1s05t,uda0yn..0d3A,0t.07.t5h1e5meagnnd/d do0fa.27y%6 mf`wegemia/glhdet abteyrtewattermeeenantttmhegenrctooungptrsooluwpaassndrsettsarpteeiacsttteiidvceaallnyl.iymsaTilghsneiwfiedcriafnetf.eiroeAnltcoentgheberetaewbnevdeinooufst.htehecornetcroovlerayndpetrhieod,0.7d5iffmerge/ncdesaiyn "agfTutwmeosr.dmoasfleiewns,gformnoucmDoatihyde,105.l57i5q(uWimdeg/eakkngd/23d)a.bylaCglcrikno-iuccopallodirsdeidgnnosftencsoeutsr,evdilvieonwttoifstohoaednismccaholendsiuunlmcepldutdiseoadcn:r,ifciochneys.ptroiaOccntteevdiapynu,ipmiallla,bdopriaeeldde reseensvlpeairrregaetadicounft,iveedrienhwfyaldasrmamdtaeittoienoc,nt.eadnOdbnyrdpeaaclyupmat1bi7eo9nn,t.thpeoCsaisuteiscoeono.nfddmeIanalteahddwiawtasisosna,dcerttihefericmaeindniemidnalatomwboaersipbcuuolnlmddonctaoonrdytihtenioentc.oruocsChtl.sinwiicAtanhl satiagxnisa.nCotaeudseoinfcdluedaetdh fwoaws nfootoddecteornmsiunmepdt.ion, excessive salivation, lsbored respiration, hypoactiviy and tMhaele0s.1a5ndanfdem0a.l7e5s imngt/hekg0/.d7a5y mggr/okugp/sdahyaddosleo-wgerrouhpighhaddelnoswietyr tloitpaolpcrhootleeisntecrhoolleasntdermolalceusrainndg ftermeaaltemsenitn eHchrooowlueepvstewerer,orleown6ol8rys%etnaheenddewf4fi9etc%httliiomnew.etrh,eByr0es.dp7ae5yctm1iv8ge2l,kyg,metaghrnaotutophtaalnwcalhseovleesltssatteiirnsotlitchafelolrcyomnsatilrgoenlisfaiancnaidnmta.fles.maTlhTeseheieneftffhfeeecctthioognnh hdtioogslhe dleinpsopirtoyteliinpolpervoetlesinwewraes79gr%eaatnerd t6h2a%n lthoawterseiennmawlietsh acnhdolefsetmearlole.s, rOesnpecdtaiyvel1y8,2,frtohme tmheeahnighhidgohsedegnrsoiutpy btihlainrutbhienycownecreentirnatmiaolnse aanndd hfiegmhaelresceornutmroblilaenaicmialdsc.oncMeanltersatiinontshethhainghmadloesseingreoituhperaltshoe choandteloowoerrotthoelr htriegahtdmeennstitgryoulpisp.oprTotheeinefcfheoclteosntrtooltawlacshorleesvteerrsoeldwwasirtev9ehwrseieedknwsiotfhriencSovweereyk.sofrecovery and th effect on vEasrtiraatdiioonl nvallyuetshewedraey l1o5w2ervailnuemawlaess gsiigvneinfic0a.n7t.5 mEgslrkogn/edavyalounesdwaeyrse62g,ene9r1a,llayndhi1g5he2rbiyn tbheecaturseeatoedf sfiegmnailfeiscanotnlyddaiyfsfer3e7n.62Trainldodo9t1hybryoniangeainvalbueecsauwseereofnovatraibaltyiolnowinerthoenddaatyasn9o1nesnodf t1h5e2seinvamlauleseswearned `sferexomeuaslp,esswweegrireveednniof0tf.e1cr5leenaatrnrldyo0dm.o7st5eh-ormseegol/aftdecadonyat.nrodlTsdhiebdruenotwtheerasepepdoeitafhrfeertroeinncbseetsanrwceeelrasteeidnnotw(0hciotcnhsheishatdoemrnitmnoionsvteerravttaiilomuneeosorfinbterhteewsetteeesndt mcaotmeprliailc,ateAsppthaereinntterdpirfefteartieonnceosf tihnethhoermsoexnuealdasm,aturity of both males and females used in the study tAot-btoerdmyinwaelisgahcripfeircec,enfteamgaelse,sainndthlei0v.er7-5to-mbgr/aking/wdeaiyghdtosrea-tgiorso.upInhamadleisn,creliavseerd-1a0bsboolduytwleiivgehrtwepiegrhcte,ntliavgeers. hwiegrhe-dionsceremaasleedsinantdheinhiognhe-dhoisgeh-gdroosuepfecmoamlep.arOeFd tthoetthweocomnatrloelss.not"sMuorvvievdi"ngluinvteirlstwheersechoebdsuelrevdedteirnmitnwaol ssaucrrviifviece,toonsechheaddulaed"msoatcrliefdic"e)anhdadlarcgeentlriivleor.bulTahrreoerofdif4fuhsieghh-edpoasteocmelalluelsar(ihnycpleurditnrgopthhyostehatthatwadsidanlosto ofbesmcarlveesdanidn2allohfi4ghm-adloesseinfetmhaelehsi.gh-Cdeonsteriglroobuupl.ar or diffuse hepatocellular vacuolation occurred in 2of 4 sNaocriPfiFcOeSmareylabteedrelveesrsiiobnlse.were observed at recovery sacrifice indicating tha the effects seen at terminal 103 a CL F J fC emli E en e Er fo Loses TFemales [Males [Females |Moles __ | Females |Males |Fomaies 4 4la Rie x ER rake i ENVAMRD(002)17FINAL P13R27O.0Sva4lu.e6so6naannd9RS.D+b7a2s.i1s35iwenrema2l2e.s2:a+n0d.0f2e6m9aliensGirnoGurpou| pma2l;e3s3a.1nd=161,98.5=i0n.m0a1l7e8sinanfdem2a1l.e4s+in1G4r9ouipn tfheemalliveesriwneGrreo7u1p.03-,+a3n3d.46i.0n3ma+l2e3s.9anidn m2a1l.e4s+an10d.850in0f+em1a3.l6eisninfGermaoluesp4.inAGtrWoeuepk4.8A0,tvWaelueeks 7w9erveal1u4es9i+n 1.38 in Group 3 males and 23.5 + 4.98 in Group3 females. CONCLUSIONS -- on aPnFdOSeffiescttoixnigclitvoercywneaimgohltgaonuds cmaounskienygsheapta0t.oc7e5llmugl/akrgh/ydapyercoaupshiyngadnedatvha,cuaolltaertaitoinonisnibnottohtatlecshtoeldestmearloels, `raencdovfeermyalpeesr.ioHdowever, the effects on cholesterol and th liver appear to be reversible after a 52 week REFERENCE S`aTlhtom(fPoFrdO,S;PJT. 62(29050)0)i.n 2C6y-nWoemeoklgCuaspsMuolnekeTyoxsi.ciUtnyauSdtiutdeydwDirtahftPeFrifnlaulorRoeopcotratnePrSeuplafroendicfoArci3dM,PoSttasPsaiuulm, Minnesotaby Covance Laboratories, Inc. Madison Wisconsin 53704-2595. April 12, 2000. S02 pp. PPSeerarecfsaleru,nocreocAatMna.dncCsounAlcmfesonnltiyrctacAtaicloindoLPfaobPtorarfsalstuioourrmyooScaRtteapn(oeTrs-tu6l2f9of5n)raoitmneC(ytPhnFeoOmSo2)l6gi-nuWsLeieMvkoernkaCenadypssSueolrneutmhTeSoaxDimectpieltreym.inS3attMuidoMynedoifwcitathlhe LDaebpoarrattmoernytRSetquudey:stTN-o6.29U52.277;9.Covance In-Life Study:#6329-223. Analytical Study; FACT TOX-030: 3M 0s ENVAMRD(002)1 FINAL DEVELOPMENTAL TOXICITY STUDIES "Title: Oral Teratology Study ofFC-95 in Rats - Experiment No. 06S0TRO008 "TEST SUBSTANCE Identity: Potassium Perfluorooctylsulfonate, CAS No. 2798.39-3 Remarks: FC.95, Lot 640. METHOD - `Maeptpehaords/0Gubiedesliiminlearfionldleoswiegdn(tioc.O,EOCEDC4D14,414, ete): Actual guideline followed was not specified but G19L6P6 (("YG/uNi)d:eliTnhees pfrorocReedpurroeduccotmipolnieSstuwdiitehs ftohre SgaefneetryaEvraelcuoamtmieonndoaftDiroungssoffotrheHuFmDaAn iUssseu"e)d, inThJeansutaurdyy, wLaabsorcaotnodryu'csteSdtanadcacrodrdOipnegra1t0intghePro1c9e7du8reGs.ood Laboratory Practice regulations and Safety Evaluation `Vear study performed: 1950 Species/Strain: Sprague-Dawley rats Numberof animals per dose: 22 Routeof administration: Gavage `DaodsmiinnigsterreegdiPmoetnas(siisutm aPlelrfwliutorhooucntiytls)s:ulfoFnoaturincgrooumpsoilofby2g2avtaigmee-omnagteesdtatSipornadgauyes-6D-a1w5l.eyDorsatess wweerree `adjusted according to the mst recent recorded body weight. Doses0:, 1.5, and 10 mkeday Sntuamtbisetriscatlabmlee.thTohdessutsateids:ticTalhemaenitmhalosodwbislel buesaesdsfiogrnaendalcyasgiessofatheccorddaitnagatroea:cDoumnpnuteetr'-sgetneesrtaftoerddraamndaonmd pcourppowreaiglhuttsea,;nCuhmibseqruaorfeffeotruspeesr,cennutmabbenorrmoaflirteiseosr.ption sits, number of implantation sites and number of RPoetmasasriukms-PeDfeltuaoirloaonctdyldsiuslcfuosnsaatneywsaisgnaidfmiicnainsttperreodtoicnolcopmaroafmlebtyergsavaangde tdoevfioautriognrso:upsof 22timemated gSepsrtaagtiuoen-D(aGwolretyerr,as19w80e)i.ghiPnugrin1a75L-a2b61ogr,ataotrydoCshesowofa0n,d 1w,a5t,eranwder1e0amvgaiklga/bldeayadPlFiObiStuom.n dTahyes a6n.i1m5alosf wtherroeudghosdeadya2c0coorfgdeisntgattoio3ncofnosrtaabnntodromsaelvcolilnuimcaelosfignsmig ofbody weight and observed daily from day 3 sBaocdriyfiwceeidgohtnsdwaeyre20rebcyorcdeerdviocnaldadiysslo3c,a6ti,o9n, a1n2d, 1t5h,e aonvdari2e0s,ofutgeersitaatnidoncoanntdentthse rwaetsr.e eAxllamainniemdalfsorwetrhee onfumibmeprlaonftactoiropnorsaitlesu.tea,Fnetuusemsobwfevreieabrlweeiagnhdednona-nvdiabsleexefdetuasneds, snuubmjbecetredoftroeseoxrtpteiroanlsigtrso,ssandnecnruompbsye.r Aabpnporromxailmiatiteeslybyonfer-et-hhiarndd osfectthieonifnegtubsyesthweeWrielsfoinxetdechinniqBuoeu.inT'hse sroelmuatiinoninganfdetuesxesamwienreedsufbojrecvtiesdce(r0a.l4 306 ENVAMRD(2002)1 FINAL skeletal examination using alizarin red. ---- er -------------- tNoxOiAciEtyLwa(sdoisnedicaantded.efNfeocsti)~gnsmoaftemranatlemaandtodxiecvietlyowpemreentraelp:ortAedNatOdAoEseLs ooff$5mmgg/k/gk/gd/adyayanfdorbemlaotwe.maAl aNtOalAlEdoLsefso.r developmental toxicity could nt be established: signsofdevelopmental ticity were evident tLoxOiAciEtyLwa(dsosiendiacnadtedefbfeacste)d~onmasitgenrinfiaclanatnrdedduectvieolnospminenmteaaln: boAdyLOweAiEgLhsodfur1i0ngmgg/ekstga/tdiaoyn fdoarym1a2t-e2r0naalt tbhaesehdigohn-daobsneorgmraoluiptioefso1f0tmhge/klge/ndsaoy.tfhAeyLeO.AEL of | mg/kg/day for developmental toxicity was indicated "1T2o:x2i0c artetshpeonhsieglhf-fdeocstesgrboyupdoosfe1l0evmegl/k-gm/adtaeyr.nal: Significant reductions in mean body weights during GD "vTaorxiiactiornesspaonndsealbenfofremcatlsitbieysodfosheelleevnesl-odtfheveelyoepwmeernetoabls:ervUneudsuianlallydohisgehgrionucpisd.ences of developmental tSthaatnisctoinctarlolrses(uDlutnsn:etAtt's1t0esmtgp/k<g/0d.0a5y),. meMainanmaltieerrnadlatbaoadnydwpeiugphwtesiwgehrtse wstearteistnioctalsliygsniigfniicfaincalnytldyiffleorweentr dfirfofmerecnotntfrroolms c(onDtruonlse(sChi-esqsutarpe<0p.<005.)0.5)N.umber of fetuses with gross findings were not significantly sNquumarbeerp<a0n.0d5)p.ercent of fetuses with skeleton findings were no significantly different from controls (ChilNeunmsbaeberrraantdiopnesrwceenrteosfigfneitfuisceasntwliythhiginhteerrntahlanficnodnitnrgosls- (-Cdheiv-esqluoaprmeenpt<a0l.05l)e.ns abnormalities with secondary oRfesmiganrikfiscantAdrdeidtuicotinoanlsiinnfmoermaantiboondytoweaidgehqtusatdeulriynagssGeDss 1t2h-e20daatta:theSihgingsoh-fdomsaetegrrmoaulptoofxi1c0itmygckoens/idsatye.d `Nmog/kogt/hdearysciognnssisotfedomaftreemdaulctitoonnsiciintythweemreeanrenpourmtebde.rofiDmepvlealnotpamteinotnalsitteosx,icciotrypoervaidluetneta,artesdoorspetsionofsit1e0s asingdniftihceanmt.eanInnuamdbdeitrisono,fuvniuasbluealmlayleh,igfhemailnec,idaenndcestotoafl fuentousssesi,febdu,t tashseymdeitfrfiecraeln,cesbiwpaerrteiten,otanstdatimsitsiscailnlgy cstoenmteoblrafeetuwseerseatobtsheervseadmeinraaltle daonsde tghreoruepfsor;ehwoewreeverno,ttchoensseidskeerleedtaltovbareiattrieoantsmewnetrreelaatlesdo. obTsehrevemdositn tnhoetaebylee.swihginochfdweavselnootpmseenetnailntcooxnitcriotly.obTsherevperdopinoratlliodnosoef fgertouuspess wcointshitshteedloefnsaabbnnoorrmmalailtiiteysionfotheonlebnoestohf ltheensaesrewaaosfstihgenifeimcbarnytloynahlighleenrsinnuhceleuhsi,ghaldtohsoeugghrouap.varAileltyeoyfemaobrnpohromlaloigtiiceaslaappppeeaarreadntcoesbewelorcealpirzeesdentto t`whiethpirnitmhaartylolceantsiofni.berAscfcoorrmdiinnggt(h0etehmebaruytohnoarl,lheisnanbucnloerumsa.liStyecaopnpdeaarryedletnosbfeibaenr admeevsetlonpmdeenvteplroopgmreesnseodf naonrdmdailslcyusesxicoenptseicmtmieondicaotnecllyudseusrrtohuantdtihneg gtrhoesasbfnionrdmiangl oemfabryleonnsalclenfutclweauss. anAanriafmcetndcrmeeanttedtobythferereeshualntds sweecrteiodnuieng1a0ntdhethmeanreanrgeanodffgrreoqsusenecnysionbswehrivcahtitohnes laennds tlheeftdiafrfteifraecntcewsaasmcorneagtetdhebydofsreeeg-rhoaunpd isneccitdieonnciensg a(Wnedtztehle l1i8m9i3t)aotfiosnismiilnahrerdeenstiginn wvaissunaoltizaibnlge tthoereepmebatrytohnisalfinnduicnlge.us. Additionally, 4 subsequent study 07 ENVAMRD(2002)17FINAL CONCLUSIONS Ghiavveenbetehenedxupelafnoaatnioanrttihfaatctthoefseecyteiaobnnionrmgaalnitditehsatretphosretefdiantdidnogssceoasusldonwoasbe| rmegp/ekatge/ddianyaisnetchiosnsdtusdtyudmyaoyf similar design, i is enirly plausible tha th lens defect observed in ths study isnot treatmentorlated. REFERENCE 0Go6rSt0eTrR,O0E0.GS., D1e98c0e.mbSearf,et1y9E8v0a.lu"aOtriaolnTLearbaotroaltoogryySatnuddyRiokfeFrCL-a9b5oriantoRraitess",.Inc. Experiment Number 308 ENVIMRDR00F2IN)AL1 "Title: Rat Tctology Say T-3351 Final Rep-oPrrojetct No. 154-160 TEST SUBSTANCE entity: PossumPerluoroocy sulfone, CAS No. 2795-39-3 Remarks: T-3351, Lot No, $0275 hi METHOD aMpepteahrosdtGouibdeesliimnielarfionldloewseidg(tio ,OEOCEDC41D4.414, ete): Actual guideline followed was not specified but gaGecLnceoPrrad(liVnrIgeNq)1u:0irtehQmeueasnltinstdyaofArtsdhseuoGrpaoenrocatdeiniLgnasbppoerrcoatctieoodrnuysrePosrfaocttfhiecthesetruOedfgyfuilacunetdoonrfseQvtuihaaetlwiwtoyefrAtshssuefriandanlcoernpaoDnredtcaewcmecbroeerrdci2on3ng,d6u1c0t7te8h,d bythe Food and Drug Administration fo compliance on and feJune 20, 197. Year study performed: 1953 SpesesStrain:Sprague.Davey rats Numberofanimals per dose: 25 Route ofadministestion: Gavage aPDcoocstoiarnsdgiinurgmeogtPhiemrefnumolorisostocarltelcyewsniuttlhroenucanotiretd)ei:dnbooFdomuyrweogiirlgouhbpytsgoafv2a5geproengngaensttaStpiroangudeaDysav6.e15y. rtDsowseesrewaedrmeinaisdtuesreedd Doses: 0,1, 5, and 10 mkgiday "wcSetoarmteipasotnuiacnladyl-smreeedttbheyoddosgnreuo-suwepady:mceSlatanastiicfsiotcmiapctaairominestoanhnasol.dyssiIussoehfdefvoavrairaainnaacnlecyse(issNeoOfrtVeheAp)rdaotvMa:eedaDn6uebnaelnhb'oomsdoygeeswnteefoiougshc,opnhetesoledvrns a`wyerecslatastsiscailyfaionfaclcyoavsaterdiiaaonscnefol(loAwNs:COBVaAr)t's test fo homogeneity of variances was pesfomed by one. 1aHForAwienNlcClesOm,oVdAAifNiwOcaaVstAiso,ingnaoinffidtchaAnetN,TuCckoOenVytrKAorwvaee.mrereeshtvomaneeunsstttygdrsoaiugpnhicfeoicma5pn%atrdiaisnfoefn-rsaeiwnleceerdeepatnr.aolayTseiestdtsyusfioonrwgolhmeoCGgaoemnnertsyaonodf apcrnoodmbaptbroialulintr-yerseevoertlpe.idonPsge,rrcoaeunnpdt tmehteeaalunvmicbboeimlrphayor,fipsdeoernacsdenfoteftfutstheeslwaoebsroev(edaenadadilaaynsdweerrbeeysonervoiannhgpaetaaersdaedma)e,hpneeersw5enatyotnAwyoN.-OivlVeArd n`aonwtdeer,thaenthaTeleyrrsepessdutlrtuass-ioJnfogFnihcskehheeCrco'ecsl"eresaxtnac-ftAo"rrmttiretesntadg.eerTtehesevt iaftolreurleiwndcaasratursteehneddo5nisentpthraeolpeeoxr,pieo0rnism.eenvteIall.fusi1itg.anisfTiiogcnarnimtfoirlcaoengntydrdeventsda `"wenausen"ttoebstsewrevreed,eovafltuhaeartetewhadstawsoi-gnlidfi.can5t%reenedlwith severe departure from ,th rests of Fisher's 0 ENVAMRD(2002)17FINAL RPoetmasasriukms-peDreftlaiulroaonctdyldsiuslcfuosnsaatenywsaisgnaidfmiicnanitstperroetdocionlcpoamraomiletbeyrsgaavnadgedetvoiaftoiuornsg:roups of 25 pregnant Sm1a9p8tr3ia)ng.gueo-SreDxauunwatlilellyytrwmaotastwuaerteedkoSsspehrsaadogfucc0l,-apDs1a,edv5.e,yanMradatts,i10nognmewgap/sedrcaosneyfxiPrpFmereOdScabgoyen,dgaweiseltryaetvipaoagniirndeaadlysuenx(taiGlmDi)cnoa6nt-fi1io5rnmsa(tWfeiotorznetloh,ef pwraessednecseigannatdevdiaabsilditayo0yfosf gpeesrtmaotiront.he PpurreisneancReodoefnat cLoapbuolraattoorryy pCluhg.owTSh0e01daayndoftacponwfaitremrawteiorneoafvamialtaibnlge atod tlhiebitmuoms.t Aredceonstelyvorleucomredoedf3bomdUykgwoeifgbhotdmyeawseuirgehmtewnatss.adDmianmisstewreerdeaonbddseorsveesdwtewrieceaddjaiulsytefdoarcsciogrndsinogf camsooprnhtsayulxmiiptayttiiaoonnndwamneodrreitbhureencdofierttdyuesadensodnwoenGrceDe dd6ae,illi8yv,feor1re2d,clb1i6yn,iccaaelnsdsair2ga0n.sn osAfenctitoximioacniltsyo.nweGIrneDdisva2ic0d.ruiaflicAbeoddgroyonswseGiDgnhetc2sr0oapbnsydy CfwoOao;sd paenrdfoprlmaecdemoenntallofdaimmsp.lanTthaetiuotnersuistefs,ronmuemabcehrfaenmdaleofwalisveexacinsdedd,eawdeigfehteudseas,ndneuxmabmeirneodf feoarrltyheannudmblaetre `rweesroerpetxioanmsi,naenddfaonrtyheabnnorumalmiotfbicsoreapnodrratheluntewa.eigEhaecdh afgeamianleawfaestehxeacmoinnteedntbsywgerroessrenmeocvreopds.y. ThEeacohvafreiteuss Bwoausins'esxeds,oluwteiiognheadn,daenxdameixnaemdinefdor evxitsecmearlally.abnAopmmparloixtiimeasteblyy tohnee-tWhiilrsdonofttehcehnfieqtuues,eswiwtehrepafritxiecdulainr iantcelnutdieodn teovtahleuaetyieosn, opfalatthee, saknudllb,rlaonn.gTbhoenerse,mavierntienbgrafletcuoselsumwne,rerisbubcjaegcet,edetxot.remsikteileest,alaenxdampiencattoriaoln tahnadt pelvic girdles using alizarin red;bonealignment and degreeofossification were also evaluated. RESULTS NmgO/AdEaLy.(doTsheeaNnOdAeEffLectf)or-demvaetleorpnmaelntaalndtoxdiecviteyloispm|emngt/aklg:/daTyh.e NOAEL for maternal toxicity is | LmyO/kAgE/dLay(,bdoasseedaondn celfifneiccta)l~simgnastoefrntoaxlicaitnyd, ddeecvreelaospemseinntablo:dy wTehieghLtOiAndELfoofdorcomnasteurmnpatliotno,xidceictryeaisses in uterine weights, and a increased incidence in gastrointestinal lesions iTnhcereaLsOesAiEnLextfeorrnadlenvedlovpimsecnertaallantooxmiacliityesiasnd vmagrikagt/idonasy, based on decreased fetal body weight and "aTnodxicforoedspcoonnsseluemfpfteicotns astrointestinal lesions, baty and 5dosaendlev1e0l two deaths m- gmlaktgeiranya;l: deCclrienaisceasl at 10 my/kg/day. siingnwseorfinteoxiwceiitgyh,tsd,ecrienacsreesasiend biondcyidweencieghtisn "eTxotxeircnalreasnpdonvissecleeraelctasnombaylideossaenledveslkel-edteavlevlaorpiamteinotnsalat: 10Demcgr/ekagsdeady.feta weight a S and 10 mg/kg/day; Sftoaltlioswtiicnaglmaretseurlntasl: enSdtpaotiisnttisc:allymesaignn_ifbiocdanyt wdeiifgfhertengcaiens,bmeetawenentotcaolntfroooldscaonndsutmrpetaitoedn,wearned nmaeteadn fgorravtihde outfedrienaedwefiegthuts.es,Noannpdarfaetmaeltrlioscsadnidanlotyosfintidhisecatmeeastnatiisntciicdalensciegnoiffitceancree;sohropwteivone,r,ottahlerreeswoarpstiaonssi,gnniufmicbaenrt cJionmeparonternetndwatoswcaorndtsriabnuteidncbryeatsheed hiingchi-ddeonscee girnoutph.eseStdataitsaticwailtlhy sriegsnpiefcitcatnot tcronetartoml.ent.Trhlaetepdriimncarreyastersenidn the incidences ofvisceral anomalies and skeletal variants were also observed. 310 ENVAMRD@OO)ITFINAL 1wR0aesbmeaorbtskrescrav--temdAednadttietthlitoen5da,lancidonnf1so0irsmmteagdtkiogofndha(uy0ncadhdoeesdqeupgarotoesultpyrs,absoaetnshosrdexutirhaie,ndgbaaltnoaod:dfyEovlviladogewinnicanelg odtfirsemcaahtamtreegnreta,alunidtcorxciioncniestiyS,daeitrnhestd ` c8alo6on-pse1ic6die.ar,eardnodtuog0hb2ea0itrwoeesart,meaanlnstdoelboalbtoseoeddryvseicdrnueast.mtehSaeing5nibfaoindcdaynt1w0ediemgchgetkagsdaeainiysn wmdeeorsaeen ggbrroeodauytpesr.WetihgTahhntescaeonnrrseodlruscittniuogrnisGngwDetrh6ee. s(prGooDsupt1se.7x:p2To0hsieunemhepeearlniogmdrg(avkGigDddt1a6ry-i20nd)e.oswSegiirggnohiuftpi,cianannttdhdeoenc1r0GeIamDsge7sl-ki1g6dmnaeyadnd0ot-s2te0lgifnrooboudtphcwohansesusmipgatnniidfoinc1a0wnmetilrykolebodsweeorryvewddhoeconne acilonlcmrrpeeaasrsteeeddd igwnricotiuhdpecson,nctweriotlisnh.gsaiTsgtnhrieofiimnctaeenasttnidnteaeclrrmlieenasaisloaenbssto.d5ayNnwodeisg1ih0gtnmsifkmigcian/nutdsydh.ifeferHrieagnchde-sdwowsieerneaenoimbWaseleisrghvateksdowineexrphrbleiogweneadrn1c11y sartoeusp,s.nuTmbweorodfamcsoriporhael1ut0eam,kagn/ddnauymbdeorsangdropulpacweemreentfooufndimdplaentoaantiGdoDn s1i7t.s among treated and contre! svStieagstinossttiiocoafnlsld,yesvoietglanloipfrmiecesanontiralplyiodntiosfx,fiecuriemtnybt eifnrrcoolmufdecdeodandtaroldfseo.tsues-erSsei,lganatinfedidceatnartlenddoecstr,oewaaashrehdsoiuangnhh,iennacnorenafsaoedfl tiwhneecsiiegdheftnfsccekfosorfwbeoarttehe S5ni2ag%nl,isfi5ca4ann%dt,fieanmcnardelae6ss0esw%eirnfeoiron1bc,so5em,prlvaeentddeicn1l0hosemuerakengofddhaey10,smrkgeulsklpgew/ctediraveeyloydb,ossceerovmgerpdoauripes,dthoeThl4eo45vp4earcinnedncNtoinotgfrhomldsa.oleeSfrtuaostusepisscawlbalusy tc2ho0enthaiinignhtihndegomsfeeitdugdsreoossupweiotgfhro1uv0pi.smcegrkSatlgaitaidnsoytmi.caallilTeyhse,ssidgeneilifaniyccelaudntdoesidsniecfxriteceaarstneiasoln,naanndthdevissikcenelcreiatdlaelnacnveoasrmiaailtniieotsnhseownfeturhmeebecorlbesofefrpvaHeldeatreis,n ' orsbiusbbeccraugvteea,dncvaoeurhsteiebsrdadelomsace,ollauvnmedn.,crpyepltviocrcghisrmdlaesawnedlliamsbsd.elSakyesleitnalskvealreitaaltioosnssifiinctahteioinbosf athnedsskuellrpcechtorwaelrgeiradllseo, concLusions ad"TebhvneeoldroepmvmeaelinoitpsamleinntteoaxylieceidyteeyveasbltnouodprymmeaenlvtietwnieestrhhonautaghlwletryheehssoeuundgyihntdtGoebsoiegrncnaanm(de19nd8to0s)rewesleiwreedren.obtuttohetbhseesnarmvaeet.desinsFutignhgdeipnsgersde4osf beingartifacts ofsectioning REFERENCE "WRetaztelT,eraLtTo.lo1g9y83S.tudHya,zTe3h3o5n1L,abForiantorRieepsoArmterica, In. Project Nsber: 4-160, December 19, 1985 an ENVAMRD(002)1 FINAL T3iVtTle-:62O9R5.A1L0,(ASRTGOUMSACRHESTUEBAER)CHDELVAEBLOORPAMTEORNITEASLSTTOXUIDCYITNYUSMTBEURD:Y O62F95P.1F0O,S1I9N99.RABBITS "TEST SUBSTANCE Identity: Potassium Perfluorooctylsulfonate (PFOS), CAS No. 2795-39-3 Rfielemdaralkosn:g wiFthOfSinal--rLepootrt2.17N,ot9e8:.4S%ampeurleot(2sSuMsDedAnianlyttwioc-ayleaRrerqautesPtF5O3S03c0a)rciAnnoagleyntiicciatlyDsotcuudyme(n-t6a2t9i5o,n Covance 6329-153), METHOD. . `MHeatrhmoodnizGautiidoenli(nIeCHf)olHloawremdon(iizeedOETrCipDart4i1t4e,Geutied):eliTnheeornequDierteemcetnitosn ooftfhTeoxIinctietrynattioonRaelpCroondfuecrteinocnefoonr Mbaesdiiscifnoralstpurdoydudcetssi,gnst(aUg.eSs.CFoaonddaDnodftDrhueg.reApdrmoidnuicsttirvaetiporno,ce1s9s94.in aFendoenr-arlodReegnitstsepre,ciSeesptweemrbeerus2e2d,a1s19t4h,e Vol. 59, No. 183). GreLguPlat(iVoInNs)o:fthTehUe.S.stFuodyodwaansd DcroungduAcdtmeidnisintractoimopnli(aFnDcAe),wtihtehJathpeaneGsoeoMdiniLsatbroyroaftHoreyalPtrhacatnidceWe(lGfLaPr)e `(GMLHPWre)gualnadtiotnhse tEhuartoapfefeacnteEdctohneoqmuiaclitCyoommuinntiegtryity(oEfECt)h.e stTuhdeyr.e were no significant deviations from the Yearstudy performed: 1999 `Species/Strain: New Zealand White rabbits SNtuumdybetorodeftearnmiimnaeltshepecrondcoesnet:rat2i2on(saoddfitPiFoOnSal.oitnalmaotfer1n9alralbibviesr,an3-d5speerrumd)ose, were included in a satellite Routeofadministration: Gavage DadomsiinnigsterreegdimPeotnas(issitumalPlerwfiltuohrouoncittysl)s:ulfFoonuarteg(rPouFpOsS)ofin202.5p%reTgwneanetn-N8e0wbyZgeaalvaangde oWnhigteestartaibobnitdsaywser7e20. A dose volume of5mikg was administered, adjusted daily on th basis of individual body weights Dinostehse:co0n,t0.o1l,,100.1,, 12..05,,2a.5nadnd3.375.7m5gkmgg//dadyay(Ingrtohuepss,arecsplecsttiuvdelyy)the numberofdocs was 3,5, 3,3 and ' "STthaetiastniicmaallsmewtilhlodbesaussseidg:nedto individual housing on the basi ofcomputer-generaed random units. The fVitatreirawnacse tTheestuniftoorfmHeoamsougreenmeeintty. oCflitnhiecalBionbosmeiravlatiDoinstarnibdutoitohne.r pCroonptoirntuioonusdadtaatwaer(ce.agn,almyasteedrnuaslinbgotdhye wpeericgehntts,rebsoodrybedweciognhctepcthuasnesg,esf,afleebdodcyonwseuimgphtsi,onfetvaalluaensomaanldy ldiattearaanvderfaegtaelsosfsoirfipceartcieonntsimtealdeataf)etwuseerse, aonfalVyazreidanucseingwaBsarsliegtn'ifsicTaenstt, oDfuHnonemtotg'esneTietsyt owfaVsaruisaendcestoaniddetnhteifAynatlhyesisstoaftiVsatriciaalncsei.gnIifFictahneceAnaolfystihse iInndciavsiedsu,alingrwouhpisc.h IKFtruhsekaAln-aWailylsissoTfesVtawrasiswataatnissctnioceatllayppsriogpnrifiiactaen,tt(heK<r0.u0s5k)a,l-DWuanlnli'ssTMeestthwoadsoufseMd.uliple Comparisons was used to identify the statistical significanceof the individual groups. Count data obtained mn ENVIMRDQOO2)! FINAL at Caesareanesectioning were evaluated using the procedures described for the Kruskal-Wallis Test NReemwarZekasla--ndDeWthaiitlearnadbbditissc(uosbsaianneydsifgrnoimfiCcoanvtanpcreotRoecsoelaprcahraPmreotduecrtss,anIndc.)d,ev2i2atpioensg:roTuipm,edw-eprreeggniavnetn * ddoosseesovofl0u,me0.1o,f51.0m, U2.5 kgorw3a.s75admmyiknigs/tdeareyd,PFaOdSusitnd0.d5ai%lyTwoenetnh:e$0babsiysgoafvaigndeivoindugaelstbaotidoyn wdeaiygsht7s-.20.ThAe adfotees dwoesriengobdsuerrivnegdthtewitcreeadtamielnytfpoerrivoiadbialnidtyo, naneddcallinyicdaulrionbgsetrhveatpioosnts-weesrtemreencopredreidod1(ihco.ugresptraitoiotnodaanyds 2r0e.c2o9r)d.ed dMaaitleyrmtahlroubgohdoyutwethieghsttsudyw.ereOnregceosrtdateidonondagyes2t9a,titohne ddoaeyss w0eraendeu6t-h2a9n;izefdo;oda gcornossumnpetciroopnsywaosf twhaesthroercaocridce,d.abdTohmienaulteraindwepreelviecxavmisicneerda wfaorsncuonmdbuecrteadndanddistthreibnuutimobneroofficmoplrapnotraatiloin,c liinveacanhdodveaardy afbentousremsa.litaineds.carAllyl afentduselsatweeerseorepxtaomnis.nedTfohevifsecteursaelsawnedreskewleetiaglhesdb,rosmexaeidteasndndextahmeinbreadinfoofroenxet-ehmaallf oinftthhee sfaetteulsleistewsetruedyf.rteheehadnodcscrwoesr-eseccuttihoanneidzeadnodnexgeasmtianteiodnidnasyis2.1, blood samples were collected, and he lFievteursewsasanwdepilgahceedntaaendwesreectipoonoelde.d pTehrelitfeert.useAsllwsearmeplreesmowveerde saenndt etxtahmeinSepdonfsoore(x3teMm)alforabannaolrymsaisl.iicAst his ime, only the liver and serum analyses have been Reported (3M Environmental Laboratory, 2001). RESULTS NmyOkAedEaLy.(doTsheeaNnOdAeEffLectf)or demvaetleoprmneanltaalndtoxdiecivteylospm1.0emngt/alk:g/Tdhaey:NOAEL for matemal toxicity is 0.1 mLaOkAa/EdLay(.dobsaeseadnodnefafbeocrtt)io~ns,maitnecrindaenlceasnodfsdceavnetlopfemceenst,aaln:d dTehcereaLsOesAEinLbfoodrymwaetiegmhatlgtaoixnisciatnydisfo1o.d0 cwoenisguhmtptainodn.incrTehaseedLiOncAiEdeLncfeosrindefveetalloapmteenattaolns.toxicity is 2.5 mg/kg/day. based on reductions in body m"Tgoxkiecdraeyspaonndsealbeoevcetsanbdycodnossiestledeolf -themaftoelrlnoawlin:g: Maabtoermtiaolnstoaxtic2i.t5ymwga/skge/vdiadyenatndatsdboovse loecvceulrsrionfg 1o.n0 aGnDd 2o2d-2y8;wienicgrhetasegdaiinncatiddeoncseesofofsca1n.t0 fmegc/eksga/t1da.y0magnkdgsdbaoyvea;ndreadbuocvtei;onsrediunctaibosnosluintmaenadnbroeldatyiwveeifgohotd consumption at 2.5 mg/kg/day and above. "2.T5oximcekreusipdoanysealenfdecatbsovbeyadnodsecolnesvieslte~ddoefvetlhoepfmoelnltoawiln:g:Devreeldoupctmieonntsalinfomxecaintyfewtaals beoviddyenwteiagthdtoases2o.5f mekeday and above: delayed ossification at 2.5 mg/day and above, Statistical results: oMfatsearnnatlfdeatcae:s aStta3t.i7s5ticmalglkygsiidgnaiyficraenatchinecdresaasteisstiincaalbosritginoinfsiwceanrcee (opbSOsOe1)r.vaetMdat3.e75rnmagl/kbg/oddayy.weTicgihdegnacienss mweearseurreedduciendteorrvablsoddyurwienigghtdolsoisnsegs othcecsue.rrerdediuncttihoens10w,er2.e5,saingdni3f.i7c5anmt(g/kpg</0d.a0y5 doorsa0g0e1g)roiunpsthaet m3o7s5t mrekdguc/tdioanys odrosbeodgyrowuepigohnt GloDssses1(0p-=103,.0S13o-r16,0.0a1n)do1c6c-u19r.edDoinsathgeed1e.0p.e2n.d5e,natn,ds3ig.n7i5fimcganktglbdoadyy dwoesiagghet graoiunpssocfcourrtrheedeinntitrheed2os5agaendpe3r.i7o5d mcaylkugl/adtaeyd dasosGagDe7g-r2o1u).psDfoosratghee-dteiperndpeenrtiordedoufctgieosntsatiinonbo(dGyDwe0i-g2h9t) a ENVAMRDQOFOI)NAIL anngdigfiodratyhedogseasgtaetigornoupp)e.rioAdvearfaegretbhoedyinwaetiigohnsowferdeossiinggnif(iGcaDntl7y-r2e%dusciegdnif(i0ca.n0t5aotrp00.001) oinn GthDe 12.75. r2e4diunctehde(30..750m5gorKgD/d1a)y idnotshaege2.g5ronupd. 3.A7b5smolaukteeadnadyedlotsvageefgoroodupcsonfsourmtphieiionrvaldueossawgeereprsiogndif(icGaDntl7y- . 21, and th ire peiod afte th nationofdosage(GD 7-29). rFeespaecdtaiav:ely)Feitnalthebo2d.7y5wneidgh3t.s7o5tdaols,agmelgeroaunpds.femSaiglnei)fiwcearnet dseiglnaiyfsic(an0t.ly05redauncded0.(0<10.i05laenrdapn<d0f1oa,l verges for ossification were seen at both 2.5 nd 3.75 mg/day dosage groups. RMaetmearrnkasl:toxicity was evident at doss of 1.0 mg/day and above. Onedoe inthe 2.5 mg/kg/day group aconndsisdienreeddoterseaitnmtehnetr3e.7a5tdmg/bkyg/tdheaystbuodyradu.thorAsl.l aTbhoertrieonwsasocacusrigendifoincangtesitnciroenasedaiynst2h2e:2in8caidnedncweeroef S2c5antmgfkceeisdianythgero3u.p7s5, mregsp/ecdtaivyelyg.rouM.eanScamnattefrnoasl bwoedrye awelisghntotgeadinisn owneereansidgntihfeiceandtolcysrnetduhceed10in atnhde 5a.s7o5cmagl/cuklga/tdeadyfogrotuhprocnamgeesnttipoenridoady(sJa1y0s-173-,211)3,.p1o6,st-1r6c-1a9umaenndt p2e1r.2i4o.d (dMaeyasn21b-o2d9)yawnedidguhragtaiionnsowfetrhee tSetcuadtyme(ndtaypser7i-o2d9)i.n thTehe1r.e0, w2a.sandsi3g.n7i5ficmayntkgreddaucytigornoupins.meMaenanmabteordnyalwebiogdhy gwaenigfhtothgaeinendtuirriensgtutdhey Spiegrniiofdiwcaanstlyasroedsuicgendifiicatnthly2.r5edmucge/ddinatyheg2r.o5upmgo/nkgg/esdtaaytigornoudpa.ysMe16a1n9,foo19d-2c1onasnudmp2t1i.o2n4,(askewdealy)aswfaosr mihge/kegn/tidraey sgtruoduyp poenrigoedsa(tdaoynsd7a-y2s9).13-1M6e,a1n6-1f9o,od19c2o1nsaunmdpt2i1o.n24,waass wsieglniafiscatnhtelyentrierdeuctreedatimnentthepe3ri7o5d (19G.D567.-2819)),an1d9t(h8e6e.n4t%i)r.e p1e7ri(o7d7.a3f%e)r,haendi21n(9i5.to4%fi)dorosaabnbgies(iGnI)ca7c.h29)d.osaPgreeggnroaunpc.y oCcecausraerdainns2e0ct(io0n%i)n.g odobssaegrevgartouiposon.ns GD 20 wer basd on 20, 18, 19,16, and 12 pregnant rabbits ncachofthe ive respective I3nMtheEnsvtierlolnmeensttuadly oLfadbooerastobryan(2g00e1)d. onAglestsateirounmdaaynd2litv1heerlsv,umeplaensd isnecrluumdiangnthaoswelefryormsepueonrtrseadtebdy pColnatcreonltsa)thiassduedsewteecrteanboletplreveeslesnotfedPFinOhS;e trheeptv.alues ae presented below. Data fom analysis of fal and ADvoesrcapgreoCuopncentratiPonROoSfPcEonOeS. in RabbitLPiFveOrSacnodncS.erumby Dose Group: ehpdn) erg scum gm) 0oi0 012519 02076530 2isn s15n 2is3s 8 57s a6 589 QPuRaOlSitiantcirveeas,edi.ncrTeahseinegeclosncoefPntFraOtiSonasreofmuPcFhOhSigwheerreinfhouendlvinrstahmapliensthoefsleirvuerm.and serum as doses of "wTahsesneotvaalvuaeislasbhloeu,lid bie vniotewpeodsswiibtlhe 0cauvteiorni.fy rI ewarsecsoavteerdyotfhatenbdeocgaeusneoursadainoa-lyltaebefleomrefseureensc.e"maMtaetrriialx CSopinkcee.ntsreactoivoenrsymainydibceatbeisastehdehiagchc.uraTchye aofnyqucaonntciltuatsiioonn pforebsecid+/-in3t0h%e.abItoristasreoporntotsedthatthat"PlFiOveSr sie ENVAMRD(002)1 FINAL was observed in the iver and serumofall rabbits dosed with the test article." Dreesvoerlpotpomnesn,tlailvet/odxeiacditfyetwuasses,eviideerntsaizedaonsdesseoxfra2t.5iomgw/ekrge/dcoamypaanrdabalbeovaem.onTghetrneautmebdearndofcocnotrrpoolrgarouutpess., Mmegakngdfeatyal bgordouypsw.eighTthe(rmealw,asfemalasloe aandsisgneixfeiscacnotmbriednuecdt)iownasinsitghneifiocsasinftilcyartieodnucoefd itnhethset2e.r5nuamnd(i3.t7e5r lavietreargeasv)eriangetsh)e,2m.5etaancdar3.p7a5s m(gl/tkrg/advaeyraggreosu)psa,nadndpuabissig(niiftiecranatnrdedfuectatlioanveirnatghees)osisniftihceat3io.n75ofmgth/ekgh/ydoaiyd gCroonuspi.derOetdheurnrfeeltaatlegdrtoosstrexetaetrmneanlt, bsoefctauissesuteheanidncsikdeelnecteasl awletreeratniaotnsdo(smaaglef-odremapteinodnesntanadndlvaorriawteirones)wiwtehrien historical control range. CONCLUSIONS - Conclusions are summarized above and this reviewer agrees. REFERENCE : `ChNiusmtbiearn:,4M1.8S-.0,12H,obJeanrumaarny, 1A9M99.., a"nOdralYor(kS,toRmGa.ch 1T9u9b9.e) DAervgeulsopRmeesnetaarlchToLxaibcoirtaytorSiteusd,yIoncf. PPFroOtSocoiln Rabbits". 3"DMetEenrvmiirnoantmieonntoafltLhaeboCroantcoernyt.ra2t0i0o1n.ofAnPaoltyatsisciaulmLaPbeorrfalutoorroyocRteapnoersta,lFfoAnCatTe T(OPXF-O0S9)9i,nFReabbrbuiatryLi9v,e2r00a1n.d Serum Samples." sis ENVIMRDODITFINAL REPRODUCTIVE TOXICITY STUDIES RPTiEEtlRSe:IENACAROTCMAHBLLIIAPNBOESODTRONARATATOALRLI(EGRSAEVPSARTGOUEDD)UYCFNTEURITMOIBNLEITRTOY:X,I6DC29EI5TV5YE,LS1OT99PU.MDEYNOTFAPLFAOSNDIN RATS- ARGUS. m TEST SUBSTg ANCE e G @e--e ---- . ee- e @ , entity: Possum Perfluroosylsulfonte, CAS No. 2765.39: p{Reuemrmptearyraktdusec:ei.tyT,Phresepiateersentdtasruaisnlpdee,cnsoimFopCnas9s5iwetro(enasoof2rt1he7ed),5twraoasoimrletceem+ipveoerndatFouenrewMiotvaheyrhnie2g0h,Stpo1n9Is9n8of,roramnadtiosnoreesdsiatngrothme METHOD -- DHMraeurtgmhooAnddiGmzueiididseTtlriapniaerointfe(oFlGDluAoiw)deedwleinr(eeiss,eadgOesECA2C.3DFb.as41iA4f,mroedttihf)ei:dcatyiTohndiosfetsiteudyreqwuaisremdeenstisgnoefdhoe UeS.vaplound IsCnH Typeofstudy (onegeneration, vo-generateotn),: Two-gencraion reproductive oxy (rGMeLHgPWu)l(oVaInnNod:fhtehe"TEhUue.rSo.sptFeuadonyoEwscanosdnoDcmroiuncdguCAcotdmemmduinisnistciyoom(pnElEiC(a)F.nDcAe)T,hwetirteehJwatephreaeneGnsooeoMsdiigLnnaiibfotircaaatfodrHeycvPtlronianscdereo(isGnLtPeh) fGLrPomrehegiinospnecstihonastdfuriinedtethcoa nductl oofir sydkyyoafhveebsetnu.docQuumaelintmyedAsorane Unik ndings se . Year study performed 1969 SpeciesSrain: Sprague Davey as Sex alesemalestboth: Both Nuofmanimbalspeerdrose: 35 Route of administration: Govage bFDeoFfsOoirSnegbsyarcegrgiafivimacgeeen(a(foiprsrtsoixaxlilwmwaeittslhypun2ri2tsid)1:oa0ysaFntidovtedaulg)rrioTnugepsmaaltoifnrg3.5srTwaesareeuratnseextdpetmrhardloousgrehtosgurctoouempsintwoaecned.sadpmnirnoiistrsoeod and laciion. Doss: 0.0.1.0, 16, nd 3.2myheldny NParfemwaeteikngfeoxrpFo1.sure period for mlesfemales (P and F1,ifappropriate Sx weeks for P; Nin anda * aBStilanytoizmsiteiadclaluDsimsietnrtgihbBoutdaisorn.uesesCdo:TnetPtironpooofurstHiodomnotgdae(tnoaediwyteryweoafenVailayrzgibeadonhdutystwiaennsgidgtA,hhnetaVlcayhrsaitnaegneocsfe VTaenrsdatnffoeordeHco(omAoNngGseUnuAem)i.pty)offwtiihne a6 ENVAMRD(2002)1 FINAL tiAhneNdiOKvriVduusAaklawlga-rsWouasplislg.insifITifectsahtnetw(AapsN<Ost0Va.tA0i5s)t,wicaaDslulnnynosetitgatnp'ipsfrioTcpearsnittatpwea,s<tuh0s.0eK5dr)u,tsokDaiuldne-nnW'taislflyMitsehtTeheossttdatwoiasftsiMcuuaslletdsi.ipglneIinfCicocamasnpecaseriwoshfoetnrhsee was usetdo identify the statistical significanceofthe individual groups. Isefevcahtleiuoranteienwgne,ercneratoguprrseaaylteddraetltaihvaefnroyr7,t5ph%reetpwiueepa,snFiwinshghiercreh'fslweEexx/rapechytsstiTicelaslltbodwmeavoserluosfpeodmu.enndtFadilesahdde.art'as DaEanxtadactpoobTstetaswitenaewdnaisantgalCbseeoshauasrveeodrasntl.o edavtaaluiantveodlbvyintghdeisKcrruestkeald-aWtaal(lnisumTbeestrofcorpora lutea, numberofpups perlite, tals to a criterion) were Remarks- Detail and discuss any significant protocol parameters and deviations: FOGeneration: dvPaaairlleuynetsdaulwreairnneigmrgaeelcsstoar(tdFieOodn),wweaeenrkdeloyonbdsulearrcivtaentditohtnewidtcareyesadtam1i,elny4t,fp7oe,rrci1lo0i,dni1ic4n,amaasnilgdensar.ttss;Bacoardnifydicwweeeeiingkhlftyesmdaaulnredinrfgaosm.oadtciEonangcshaunmddpotstiahogenen o`fgrmouapticonngsitsitsedgorfoutpwowassetsdoosfefdeumntaill graetss.tatOionnedsaeyt c(oGnDs)ist1e0doanfdthdelfirvsetretdenvifaemCaaleesarraetasnw-istehctcoonnifnigr.matTihoen wtrheaemsadieanvmaislnugawtfeeerdmeaaletevsalelcauosatmtpterwdiisfcoeerddctalihilnyisceafolcrosnsidigznsesetadnwudhriipncughpdpaevriltaiubvrieliritetidyonnataaunbridratllh.ye.ngPtDhuuporfionbgsgeesttrhavetait2oi1no,-ndsaanyddulratichnteganttichoaencp2he1rl-idoeadr,y oulnanclftoyaltdoiinonngl,apceetryaiteoidoonpiednnacilynugd2,1e.dacpoOuhsnytsilicaccasttaastriitoglnnesrd,easbypoo4d,nyslewiteaeinrgdshtaiws,erreniugrrhasininndggomrbleefyhleaxcv.uilolrPe,dupsituolrffcaoocunesrirmriiagclhtetiioanngnwdarefsfolueervx,aflepumaiatnleaed npeupcsr.opsTiheed arfeemraitnhiengendpuopfsdowseirneg saatcrtihfeicteidmeaonfd pnaretcurroiptsiioend.(actTthieonFdOayma1l).e prostate, and seminal vesicles were weighed. Evaluations of sperm number, Trahtes tweesrtees,seapcirdifiidcyemdidaensd, motility, and morphology swaecrreifincoetdionnclGudDed1i0n atnhdpnreoctroocposli.ed.ThePrFeOggneannecryatsiioantufsewmaalsecsotnhfitrdmeeldi,vetrheedobvyarCieascswaerrceanesxcacmtiinoendingforwehree fnourmbvieabrilaintyd. diTshtreibFuOtigoneonefrcaotriponorfaemlautleeas, tihmaptladnetlaivteiroendsintaetsurwaelrley dweetreermsianceridf,icaenddoenmblarcytoatsiownerdeayex(aLmDi)ne31d cawonaldslenrceetcceordrodpfesrdio.emd.ThmOeavlalierviererastfsrwtoehmraetcahecaxhdampmaiarnteeendtdaalasnadatbfworvaoesm ra$enmdfoetvmheaedl,enruawtmesbioeghrneadLnaDdndd2i1satnfraoilrbyuzptehidao.nrmoaBfclooikmoipdnleastnaitmcaptlaienoasnlywsseiircse:s Vives from the pups from the liters ofthese five dams were aso collected for analysis. EGleneration; oSnilnycethFeI 0g.e1nearnadti0o.n4pmugp/kvgia/bdialiytydowsaasgesiggnriofuipcsanwtleyrerecdaurcreidedinintthoet1h.e6saencdon3d.2gemnge/raktgi/odna.y dTowseangteys.fgrwoeupFsl, p`zmeegnre/srkeagxt/idpoaenyrbrleaitgtsienrpneiirnngsceaoxcnhpLedDrosd2ao2gseaengdgrrocououpnptwiwaneusrientgesattdhemdrioniuingshatetprhaeesdsdiaPvyRebOaeSvfoobirdyeasngacacreviafpgiacere.aadtiAgdtmo.2se4sOdanoyfsL0o,Df0a2.g81e,,,afonendmeal0re.as4t pwreerpeuieavlalsueaptaerdatfioonr.theOangeeorfatvapgeirnsaelxppaetrenlciyttearndweorne LevDal3u4a,temdaline aratwsatweer-rfeilelveadluMaotmedazfeootnheLaDge70o.f wATeshrseeiFgcInomngesenintdeertraoetidcootnhoabmbeiatlaaettiroGantDswwi0ethraienndseaaacsrcsihifigdcnoeesddaagofeegirnrdmoiauvtpiibdnuegag,lannheocournsoipnsLgiDefdo9r0a.ntdhFeervmeaamllaueiasntdewedirtahsoftdeehvsiecdredinobcseeidnoigf pmteahrteiio1nd0g `generation. All FI generation females were allowed to deliver naturally. Dams that delivered liters were 3 ENVAMRD(2002)17FINAL aLcDr2i1fcaesdparnedvinocusclryodpessciroeindbeLdDor21t.heAFlIl gFe2negreanteiroantipounpsp.ups were sacrificed, necropsied, and examined on ui Lo gmNeaOnleeAraEatLniodn(fdmeoamslaeelaepnapdraerneetfnaftelaclta)nai-nfmiaomlarslsFcOo=,ul0Fd.11,noatmngbd/ekFgd2deta(eyar,smiatnpheepdrlosopiwrneicseatttedr:oesateTmhetneesttNerdOe.lAatETedLhesfioNgrnOstAhoeEfFLt0oxbgieonieysrhaewteipro1en gmoegbn/sekergra/vtdeiadoyn.aotf0Tf.sh1peraniNdn=OgA004E.1Lmmggf/kokreg//tddhaaeyy.;F1thgeenNerOaAtiEonLoffofrsptrhiengFI= g0.e4nermagt/ikogn/dfaeym.aleThpeareNntOalEaLnimfaolreth--e 0ro cmLoaOnlsAeuEmapLnido(nfd.eomsaelTeahnpedarLeefnOfteAaclEt)Lan--fifmooarrltsFhOe=,F0IF.I4,gmeanynekrdga/tFdi2aony(,amsablaaepsperadonpiormniaalrtseed)u=:ct0i.To1hnsemgiLnkOgbAiodEdayLywfeboiragsthehtde goFanOiniggeannniedfriafctomionodtn pomrfuegfdp/sukpcvgrit/aiibndoiganlsyit=ybi,an1sg.e6radobmswgoot/nlhkurgte/edduafcyot,oiodbnascsoeinndsbouonmdpsytiigwoneniif;gichtathnetanrNdedOfuAocotEdiLocnosnfiosnrutmthpheieonnuF.ImbegTerhnoecfrLaitOmipAolnEanLtfeafmtoairloent.hseiatFneIis,mgaeetnerer--astii0eo..n4 significant reductionsainndpusuprvgirvaolw.thThe LOAEL forthe F2 generation offpring - 0.4 melke/day, based a " fT`TooooxxdiicccocrnfefsscuptmospntsiineofFnfOeactgtetnsheerbay1tid6oonsaenadnliem3va.el2ls:-meprkaeyrdudecanttyioandlso:siangbeotghrbooudpsywdeuirgihnttghaeipnsr-amnadtiinngabpseorliuotde. anFdolrleolawtiinvge fTmaeetrpimrniognd.aulcfbiooovddtyocwioecniisgtuhymtipsnttwiheoerneFwOalagsseonsseiirgganntiiiffoiicncaamnntatlllyyesrerwdeeudrcueecdesdeineintnhaetht1.6ehe0h.4i.gh3ea.sn2tdmdgo1/s.6ek.gg/mrdogauhypgdoodsfey2g3rdomouspsae.ge Sdgiargyonueapnoedf rAienpcoslriutgedndei.dficsaiTnghtneifiionccnarlneytasrfeienddiunicnttgihsoenrsneipunomrtbhteerdaibonsfotmlhaeultFeOswediwgiahtmhtssoborfcoctuwhrneresldevmieinnaattlhe3ve.0s.24i,cmlge1s.k6g,(/waidntadhyf3ld.u2oimsd)egagknrgdo/utehpaeywperdeeostssuargeee. w`erioguhpts aanndd ifnocoldudceodnlsoucmalpiizoedn gestation and lactation. alvoapleuseiaodbuserrivnedprdeu-rmiantgintghegepsrtea-tmioant,inagndpelraicoidioann;d cnodntrienduuinegsotnhsroinugpheocust aTFboIsxoiflecumtaeelfeffseo:toosdbiscneorFnvIsautgmiepontnesiroaantitoohnne0pa.no4ismtamwlges:s/nidFnIagmydaaldyeosss;a1gt-he8egoorcnocluuyrprriiennpcgolruattdeetddh,eefr0fe.ed1cutcsatniwdoenr0se.i4simbggan/idkfyigc/wadneatiygrheddosuscoetniJodennascylis|n opnosdtawyesan1i-n8gp,ossitgwniefaincianntgoansdssduirninbgoldayctwaetiiognh.t on LDs 1-4, and significant reductions in food consump i Toxic responseieffects by dose level offspring (FI/F2): m(Tog1x/6ikcga/neddfafy3e.dc2tomsigenlgktrghodeuapFy,I).wgietTnhheroeavterierodn2uc6pt%uipoonsfstciohnnespipusuptpesdvfioaobfuinlridetddyuebcaeedgdabpneuttpowveaipanbpieLlaiDrty2o-an4t. LthDIen t3twhpeoo3sh.ti2eghlmelgsi/tnkggdo/1sdeasgyrdoou1pss6e gVirinoacubripelais4tey5s%awnedorfeltahcoetbaspteuiropnvseidwnedirinceetsfhoewuennrduedmebsaiegdrnoiofnifcaLdnDatIlmy;srnweoidtuphcuepsdsiilsnulrbtvohimevespduspbasemyeaotnddotshLaDg3e.21.gmrogSuuptsgis/(tdviaciyaatbdyiosysiegemgfrniocuupn. s0i%gniaftic3a.n2tmreydkucdtiaoyns ainndpu6p6b%odats 1w.e6imghetsgwedraey;allsaoctoabtsieonrvienddeatx th94t.w6o %hiagthe1st6 dmogs/akgg/edgaryo)u.ps.SuGiesstcaailoyn sis ENVAMRD(2002)17FINAL islnietnetgshtehfo3wl.la2oswmesgdi/bgknygif/aicdcoaanyntcldoyomsireetdalunecvteeldreaadtsusc3o.t2ciioanmteigdn/lwdiitateyhr.sriezdeuSwcitaginsoinfoisbcsaiennrtvpreuedpduatvcit3ai.bo2inlmitigynkgathsnedaynm.uamtbeOretnrhaelorfcaaidrmvepelriasnnecthasutiiigeondne cJiatntnerisbawlizthatpiuopnosftphaut pwertheatnowterneursstiinlglboorrnwohrofohuandnd odeeavdi.denceofmilk in the stomach, as well as maternal mb{aTesoaixnsi)cpoeufbpfseebcrtovsdeyidnwatethie0g.h1Ft2smwggee/nkreger/oadbtaisyoenrovpneudpLsDonc4oLnasDnissdt7e7-.d14o.Aftr0e.d4ucmtgi/onksg/idnaym,eastnatpisutpicbaloldyysiwgeniigfhictasnt(ornecatpieornlseirn mFStOkategiesdtnicacrayaltiraoennsdulmthasil:gehera.nimaAlbss:oluSitgeniafnidcanrtelarteidvuectfiooonds c(opns<um0p.t0i5oonrvpal<ue0s.0w1)erien sbiogndiyfiwcaenitglhytrgedauicnedat(p0.<5 w0ne0urm5ebeocrronposfi<mdea0rl.e0ed1)rattoisn ibtnehetthr1e.e63a.ta2mnedmngt3/.kr2egl/madgtae/ydkgdb/oedscaaayguesdeogsrtaohgueepigwnricotuihpdse.bnrceosAwnwseilirgvneeirdf.iocsaTnahtgeeingdcreropeseasnsdeleen(stpi,o<ns0o.Sf0i1gtn)hiefiinlciatvnheter wdrioetsdhaugcfetliugiordnoau(pnpsd.<thS0ei.gp0nr5oisfatinacdtaepntwr<eerd0eu.co0t1bi)soenirnsvt(eedn<mini0nt.ah0le5b3o.or2dpmyg<wke0.ie0g1h)dtaisyndwtoehsreaeagobebsosglerurotvueepdweiingthhtes o1.6fthaendse3m.i2nmalg'vkesgidcaleys boFboOsdeygrevnweeerdiagtihintotnhgeafie0nm4sa,laen1.da6n,ifmaoanoldds:3c.o2nSsimguynmkipfgti/icdoannatyi(dnpocsr<aeagse0es.s0g5(ropour<psp.0.<0Si0go.nr0i1pf)icw<anet0r.er0e1od)buscietnriovlonecsdaliininzbetdohdeaylo1wp.ee6iciagahntdwae3nr.2de dmugr/ikngg/dlaacytatdioosn.a"geSidgunriifnigcapntrermeadtuicntgioannsd(pge<sta0t.i0o1n) were observed ai 3.2 mg/kg/day. ainndgetshteantioinn 0le4ngtmhg,/kigmp/ldaanytadtoisonagseitgsr,ouapndanTderabsoivzee dmFgIa/mkgsegwn/iedrtaahytsitdoionlsloabfgofresnpgrpriuonpugps:s,.wPhuiSpligevniSiaifbgiinlciiaftniytcwainantscrreseaidgsunecistfii(ocpnasn<t(lp0y.<0r5e0d.ou0rc5peod<(p 0<.<0010.).001w5)eworeerproeb<soe0br.s0ve1er)dvieindntithnheeth1.6neuvmaibanebdirl3io.t2fy ri1ne6ddeun,cdedl3ap.c2utapmtigbo/onkdgiyn/ddweaexyi,gdhaotnsdawgaaevsegreravogiudepessn.tforinscuarcihvidnogsapguepsgraotu3p.,2wmigt/hkgst/adtaiyst.icalAsdiogsnaifgiecadnecpee(ndpe<nt0.p01a)ttienmtohef cF1oI-4nsgaue1mn0ep.rt4aitomingo/naktga0/d.du1altya:nadanin0md.a4lssim:ggn/iMkfiagc/ladenasty;r-efdSueicgmtnaiiolfniesc-sa(npsti=grnei0df.ui0cc5at)nitoinn(spfo=(opd0.<c0o50n).s0bu5omdpoytriwopeni<gatht00..l04o1s)msgoi/nnkgal/badscoatalytuiotoenn ddfaaoyyossd. F128gpeonsetrwaetainoinngo.ffspring: lactation day 4 and 7. but Pwueprebocdomypwaeriagbhltes ttoecnodnetdrotlos bbey rleadcutcateidonindatyhe 140..1 Pmugp/kbgo/ddyaywediogshatgse ignrtouhp 0o.n4 smigg/nkigfi/cdaanyt controls. rdeodsuacgteiongsro(upp<te0n.0d5edantod bpe<re0d.u1c,edr,estpheoctuigvhelnyo)t osniglnaicftiactainotnlyd,ayosn 7laacntadtio1n4, daasysco4m-p2a1,rewdittho RmRoeerpmtoaarlrtikteysd,:aenfdfIencntotsheefFifneOccltugsedneoednramrteiadountctmoiiaorlnnosegnrianatsn,byootthfherhebeowdefyerretiwlneiiotygthprtaeraagtmameietnnestr.sraeneldvaatleiudnactaleibdnsioicnlaulatnesyigadnnosdseofrgerltaooxtuiipcvietteys,ftoeonddo wfceoooindgshuctmospntwsieuormneptaatilotsnhoews1iag6sniasfinigdcnain3ft.il2cyamnrgte/ldykugcr/eedddauycineddtohsieangt1eh.e6gra0o.nu4dpas3n.dd2urm1i.yn6/gkmetghi/edkapgyr/edd-aomyastediognsrgaoguppeesr.giroodu.BposF.doylTlweoerwimiginhngtasml,atbbioonddgyy. `dwroeosiuagphgteoggfarionu3.sp2,.magbSsi/ogdlnusateyofaanrndedprrieolndacutlciutvdieevdfeostoiodgxnicicofiintcysaunimtnprtteihdeouncFtwOioengrseenieunrnatathfeifoesncbtsmeoadllubetyse wtwreeeiragethmtessneotefnattahttehteshee0m.ih1niagmlhge/vsketsgi/dcdolsaeeys 319 ENVAMRD(2002)17FINAL m(wgit/hdfaluyid)doasnedgrthoeupprwoastsatael.so rAeposritgedn.ificant increase in the number of males with brown liver at 3.2 pfIaonrrtahCmeaeetFseOarrsge,eannte-hrseaetcnituoimnobnfieenrgms,aolftehceroartresp,doniroddaenoaltutthaeapspaewnaedrre1irmepbpleoarnattenadtyiaoetnfsfa,encyotsrdooisnnetclhseetverlon,uusmTbcnyecdrlainomgfs, vsimaaacbrtliiefnigoceradnnodonnfGvriDislbl1ly0 ``ewgmerbiorguyhptsosaa.nnddTihbneocdloyundlweyediflgiohnctdailngigazseidnarelaponopdretcefidoaiodductrohiennsgFuOpmrpdeta-immoasntiovncagcl,uugeresestdoaibtsieonrt,vheeadn0d.d4u,lraic1tn.a6g,titaohnne:d pa3.rn2 ed-rmmaegtd/iukngcg/odpnaesryiidondobsoaadmgyie continuing theoughou gestation and lactation (aRdbeievlleiartyyssi0bilnestudrhefelaacye3.s2rmiiggnh/trkegwf/ladesxaysaindgdonsipfehicygasrniotculaypldwdeeelrvaeeyleaodbpsimcnerntvhteedw1oe.nr6eLaIonDbds13e,.r2avfemedgr/iwknhgti/hcdehayFtIhdeogrseeangwereeargteirnoonousposufrfovsnpirvLiinbngs.g p3Tu-hp1es0 atrbheiemlait1iy.n6i1n0mggs/ufkrogfra/cfduearytrhidgehrotsoabisgneerdvogasrteoiougnpr).ohuapdsByt<het0h.ea4biemlngidt/ykogtf/odtashyue.rafbicSseicmrrivilgahattr.ornesTppheoreinrosede,swehwroeewreenvoesre,deenallfaloyssuporibvsinevarinvugendfpouilpndsitnhigen Tcanrhdoesetsyiemaleolopdfeondsieenvgeg.rlooupApmlse,tnhbotyuogtfhhetthheeenraedcoowuefsrttiehcetsrotabnassreierenvtartedfileoelnxapyaesnrdisoetdenhrewasibptiohnlsytehse0sieanistpiugipngsshtowfewreperhyessiibmcoiatllhardse1i0vgnecilfooinpctamrneotnllty 01r.e04duumpcge/odfk0gi./n1dtahmyegg/1rk.o6gu/pm.dea/ykAgat/ntddhaeoyednondasaotgfrlealnagscriytoeauntpti.odne(NlLaoyD2(e1fo)fne,LctaDsllo1in6vtoenhlpeyus)psrienifntlheaexlleasdbiowlseiertyegtrooobuaspresrrv(i0eg,hd0.t1,iwn0at.sh4es,cleoanwnidndot1sh.ee6 mikg/day) had pupil constriction response. do"Toshsecemggrsrootuupp,ssi.gwniitTfhihcevanertredfu2icn6tdi%ionnogfstrheinpeoprputueppdsviifnaobtiuhlneidtoydfebfasepdgraibnnegttwoweaaespnpteLhaDaro2of-n4r.eLdDIunct4ehedpop3s.ut2pcmagvliiaknbigliiitnyatadhteotsh1.6ee tmgwr/oouehpigd4h5ae5sy%t roiefndatethxleywparuepsdsuawlcseeodresiinfgontiuhfneidsceadndetoalsydarogeneduLgcrDeod:up(sn9o4(.p06u%%p)satisnu3.rt2vhiemvge1d/.k6bgde/oydsoaanygdeaLngdDro6u1.6p.%AsaItna a1.dr6edsiumtlgit/o,kntg,h/gdeasvyit)aa,btiiloiTnthyleeinnlgdatechxtawwtioaossn Pisimugppnliabfniotcdaaytnitlowyneisrgiehtdteuscfewodelrlieonwatelhdseobhyiogbahs-ecdroovnsecedomagitrtoatunhpte traenwddoucththiiegorhneesiatnlsdiooeswraagsseizager.soiugSpntsia.ftiicsatOnittchaelelrdyuascditgvineiorfnsieciansnittghnresednunucmttibhoeenrs5.io2nf cpmauenpknsieb/atdlhaiatzyatwdieoorsneeoflneopvteulpnsuasrtsshoiactnigawteoerrde wwsithtiohllbrhoeardnducontrioofnoesuvniidndedpneucaped.voifaTbhimeliiltpkyeraicnnednttmhaaegteesomtfoamlmaacclhae,repauispnscwlewuladlesdacsloimtmtpeaartsreawmbialhle across all dosage groups. oSnilnycethFeI0g1enaenrdat0i.o4nmpgu/pkgv/iadbaiylidtyoswaagse gsirgonuipfsicwanetrleycraerdiuecdedinitnotthhee 1s.e6coannddg3e.n2ermagtikogn.day dosages groups, bwCloeidrnyeicrawelepoiorgbtsheetdrsvabanotddioynnoswesitinagtthihstetigFcaaIlilngyse,sniegarnvaietfriiaocgannetmddaialfyferoraftesnpcarepesppwueteairraeeldrseuepnporarertmaeatdrikofanob;rlaev.nayluoNeftsohfteorrefaoltlelmaoemwniitnnrgge,lptaserhdaomrdtee-tateterhrssm rwreealttaeetnirtvimeoawnze.eilgophnetgrs-ftfoeorrrmmtahrnecteernipgthaitroonaedoirfgrme;stpmesoatntissi,engsienohmiribnifaetlritoivnleisatiyclepevasar,laurmiaegttheetdrsb;ynpeecrrfoosrcmoapincceexinaminpaatssiiovnes;voaibsdoulnucteeoofr einpiadbisdoylmuitse,ooordprcoosntastuem;ptainodnteornmipnoasltwbeoadnyinwgeidgahtyss.1-8Tohcecounrliynrgepatorttheed0e1ffeacntds0w.e4remgs/ikggni/fdiacyandtorseedulcetvielosn.s. BY) ENVIMRD(2002)1 7FINAL mCblogidn/yicdawlaeioygbhsdteorsovanagteiLoDgnssrfoou1-rp4,itnhacenldFudIesdig,gennrieferidacutacnittoinornfesedmuinacltbeiosodnyweiwrneeifgolhoitdkeswcoiosnnesduuamnyrpet|miaporonksatobwnleead.naiynsOgb,s1e-s8rigvpnaiotfsiitocwnaesntaantliontshgeesa0n.id4 depavuraralimuneagtteelrdasc:btaytipoven.arlfuoerTsmhaefnrocerewlienerameipnnagos,isvsaehtoiarsvtto-iitcdelarlnmycersieogtrneinwftaiicotanen,rtmladozinefg-fptreeerrnfmocrermeartneepcnoteripotneadroaffdoirrgeamsnpymoantosifentgihneafnifdboilifoleno.rwiinyg parameters; gestation index; pregnancy rates; and necroscopic examinations wcEoevniitgdrheotlnscle(eoovnfelaseapbteyrmeLintDte-rr14eb.luastAset)d0o.ebf4sfeemrcgvt/sekdgi/antdta0hy.e1,Fms2gat/gikesgnie/crdaaaltyliyoonsnipgLunDipfsi4ccanonntdsirs7e.tdeudBctooidfoynrsewdeiunicgtmhietoasnsnweipnruepmecboaomndpyapruwapebilbgeohdttsyo cwaolentrthreooluosg)bh.stehrCvleiddniifcofanelreaLnnDcdse nw7ea-c1st4o.nsoctoMpseitcaatnoisbtbsicoeadlrlyvyawsteiiigngoihnftsitscohafeontnF(2L4D6g.2e51negrcaiotnnito0in.n4pumeudpgsktwgoe(rrdeaemyauindnroesmleaocwgkerarobulteph.avne.Nc5oo0nottgohlei,rn toxicologicaly significant effects were reported Lwievreeraannadlyszeerda fsoarmtphleesprceoslelnecceteodffPrRoOmS.theTihnietiFalOproepsuulltastifoonr tohfedoFsOeadniamnailmsalwser(eF:O) an theroffspring (F1) (Dmogskcggirdoauyp) AvinersaegreumPF(uOgSmlco)nc. Avienriavgeer (PuFgO/gS)conc. oo: mfaelmea0l.e0204.40307 0 mfaelmea1l0e.5528 0a fmeamlael4e5.1489 16: mfaemlaele15822 32 mfaelmael2e7N3R* *samples not received femamlael0e.06.61571 fmeamlaele54.149.5 mfeamlaele1756.5.0 mfaelmeal3e21384 mfaelmaele13N6R0 `tahQneudaslletivraeutrmisv,.layP,nFdtOhmSealcFeoOsncrheeanstudlrtagstreiiaontndliyicnaictnreceraaeslaesdaetdwsiPt(hFiOdnocSsleuc.doinnPcgeFncOtorSnatrtcoiolonsnc)senhitnardsatedireoutnemsctawanebdrleelihlvieevgrehlewsrheonfntPchFeomOlpiSvaerirnetdsheawnriutihmn ofcoewnmecaerlnetsPorFaftOtiSohnecsosniacnmepneotrdoaoltseiedonglsriovutephr.asnaPamdopuollletessdoflfrvoetmhresFFIuOmpaglneeinmsearflasrtoismohntohoreflyFtlhaaefnesiramambleistdshoascwereirfgrieocue0pd..0s5ho1Tr1ht,ley6.aa1vf9ee,rra5gb7ei.t6P,hFahOnaSdd q7tuh0ae.n4tPiutFgaOtgiSoinncitohn+ece0n3.t00r%.a0t,.i1ot,nhe0.ipn4u,ritathnyedolf1i.6 vtehremyaa/nnakdlgy/tsdieacrayulmdroessfeehrogeurnlocduepssb,uebrsvetsiapenewcceetidviselwuyi.ntkhnTochwaeunst,ieoanq.nudanttihTtehareteivwaeecrcveualrsuaeecvsyerfaoolfr uncorrected dilution rors. coNcLUSIONS ee eectseterna `Conclusions stated above and tisrevieweragrees. = ENVAMRD(2002)17FINAL REFERENCE = CNhurmibsteira:n, 4M18..S0,08H,obSepromnasno,r Developmental andPerinatal AStMud,y aNnudmbYeorr:k,62R9G5.9, 1J9u9n9eb.10,Ar1g9u99s,ReCsoemabricnheLdabOorraaltor(iGeasv,agInec). Postnatal Reproduction Toxicity StudyofPFOS in Rats FPerrottiotctoyl, a3LniMvdeErCnoovnficrSeonpntrmraeagtnuiteoa-nlDaLoawfbloPeroyattRaoasrtsys.iuEmx1p9Poe9sr9ef.lduAtonoraoPloyFcttOiacSnaclvsiuLalafGboaonvraaattgeoer.(yCARAesSpoaNrmutemnobdneertdh:eAp2D7re9ti5e.r13m9,9i-n23a0)t0i0io,nn otfhethSeePrruemseanncde 2 ENVIMRD(2002)17FINAL CROSS-FOSTERING/DEVELOPMENTAL TOXICITY STUDY. LTiAtlBeO: ROARTAOLRI(EGSAVSATGUED)YCNROUSMSB-EFROS4T18E.R0I14N,G3SMTTU-D62Y95O.F13P,FJOUSLYIN1R99A0.TS - ARGUS RESEARCH I TEST SUBSTAANNCE Tdentity: Potassium Perfluoroactysulfonate, CAS No. 2795-39.3 stRteermempnaegrtrahkt,sur:ceo.mpTohPsreietpitaeosrnte,daarsntiudcslppeue,rnisFtiCyo-onf9st5hw(eeLroteetsst2a1tr7t)oilaweraisrseooordnmecieetievmewpdietrohanttuhrOeec.stpoobInensrfoor2r1m,ati1o9n98r,egaanrddisntgortehde eatntritoyo.m MAA0N 0 nAn RATA tIMhneettehrUnoSaSdt./iGonuFaioldoedCloiannnfederfDeonrlculeogweoAdndm(iHinai,rsmtOornaEitzCiaotDnio(4n1F:4D,AGe)uti)dw:eelrieFnoeursoetndhe adcsertoetschste-ifoobnsatseosrfinotgfoxstithcuiedtyys:tutTodh.yerderpeerqeoundiur,cetmfieconn.tstfohoefr UimneS-dliiFfcciDnpAahlaGspLeroPadunFcditnsta,hleFReaundlaeelryat2li1cRaCelgFipRshtae5sr,,e Sowefipttthheimesbxsecterup,dtyi2.o2n,sT:1h9Te94wA,oBVsaSelp.afri5na9a.tleNesopt.ourd1ty33dd.iorcesFcotnroortsthweheaPrveeFOaaSssSicbgitnoeeamdneatlnoytstiohsef `C7oG9m2L,pPlriraaetgnhucelera.ttihoanTnsh;eFaDnQaAlAy2Ut1icsaCtlFatmReemtPehnaotrdi5nw8.atsheDnooAstBefSuclolfnyifnaivlmalmiradetapitooerdnt.ainNnadolitycasateleslsrwceowrmepdlanitoaatnwccoeenrdewuitcvehtreiEdfPiaeAcdbco4r0ditCnhFegRg10rPotauhrpet leader or designe. CrGeoLgmuPmluant(iiYot/nyNs.):ofTthhTeehreeU.Sws.etruFedDynAo,wadteshveicaJtoainpodnausncctfseredoMmiinntihsectoGrmypLloifarnHecegeaullatwthiitoahnnsdtthhWeaetlGfaaofrfoeed,ctaeLndadbthoterhaeqtuoFarulyirtoyPpreoaarcntnitEcgecroin(toGymLiPoc)f the are dsotucduym.entQeuda.lity assurance Unit findings derived from the inspections during the conduc of ths stad Year study performed: 1999 Species/strain: Sprague-Dawrlaetys Numberof animalsperdose: 25 Routeofadministration: Gavage ib`nDetogosidinanngyin2rg1eg4oi2fmdelanaycst(aiptsirtoinao.lltowimtahtiunnigtst)o:unTrweosgtro(ubprseoefd2er5) mfaelmeasl,earantds wcoenrteinaudimnignitshtreourgehdoPutFOgeSstbaytigoanvnagde Doses: 0, 1.6 mg/day Statistical methods used: aApvperorpargieatse.and percentages were calculated. Litter values were used where Remark-s Detail and discuss any significant protocol parameters and deviations: Two groups of25 23 ENVAMRD(2002)17FINAL ibfneetgmoianldneaiySnpg2r14a2goufdea-lyaDscatawptrliieoonyr. rtaotAsmawdteoirsneegavdtoomliununimserteeoraefdd0(mbarLnekeddge1r.)w6ammsaglae/dsm,dianainysdtePcroFendOt,Sinauidnijnu0gs.tt5eh%droTduawgiehleoynuot-n8g0etshbteyatbgiaaosvnassgeno,df rwieencdrioevridrdeeudcaolornbdceoeddyd| uwhreoiigunhrgt.ptrhiePoararcteconltiaamnladtfiaofnteerpedmrowiseoiadrnegalnoddbusreteihnregvsnetddhaeitlwtyircedeaudtramiienlngty tpfheoerriotvdri.aebaiulMmiaetntytearnpnedarlcilobidnoidacynaldwoeabtisgesrahcvsraitwfiieocrnee:s caflnoidondioccnaodnsaisgyunsmsp1.tainod4,na7lw,saos1f0o,arlasdnodure1c4rorooadfefldgtaecostinattcaietooni.donunr,iDlnuegrnigtnthghe poaacfrcptlauirrmtiatuirtoiinto,inonfp,eemlraiitloeedrsiawzneedrs,ethacenonndtdipaniuulpaylvldiyuarbeiilvniagtlyugaeattstebaditriftoohnr vdMiiasotcxeeirrdnaealwaasbspehhypaxeviriafotoriromwneados;nardneaycoygrrd2oe2sdsodflaellsayicotntashtirwooenurgaehnodpurtaeslgearrcovasetsdonfneo.rcrfoAulptlsurymeaoatfneartlhmyesailst.haotrsaIcniwcae,drdesithsidaoocnmr,iinftiahcele,dnuabmnybdecpraerlabvnoidcn `cdiresoxisarsmi-ibfnuoetsdoenrtioonfgcoipnmrfpolicarenmdtuatrtheieoanpnrdietswecesnrcweeelsraaecbrsrieefncioccreeddeodof.n ilmRapaclttaasntittoahnattidodaniyds2in5to,st.edxealDmivaiennresda wflioitrthegrrnowsoesrseluersnivooitnvsii,nngcalnupddueptdhseiwnuteterhreie sacrificedafter the last pup was found dead, misosrpirensugme,d cannibalized. pfFlooualrcleogdwriownuigptshcoocimftph1le2er-t1ai3ocndoanormfoslop-arrtopurruipPtsFioOnaS,stflriotlatlteorewssd:wderAae)mcifomonrmterrdoeliaarditnaeglm.ys Trwheiitmshovclreiotdsesrf-srffoosrmtoemtrhiPenFgrOprSre-ostcpeeeacdttuirevederddeasamumslst,edia.cn.idn PtinrFeOauStte-edrtoedaaetxmepsdosdwuiartmehsolnwilityte:hrsBl)ftrrcoosmntfrProFolOmSdc-aotmnrtseraowtlietddhamdlsai,mtse.,s.,if.pr.oo.smtbnocatothnatlionlexudptaoemsrsuo,raeinr.dl..yp,onsetg-antaitvael ceoxnptrooslu;reC:)aPnRdODS). adOnunormidanalgiyelsa1.cotfaltPaiucotpnafbtoiorodvnyia(wbsieiliictghyh).,tscPawucephrsepwurepercewoarodsbesdienrodvinevdiddauayanslcle1y,dw4a,eiil7gy, hf1eo4dr,caalnindndice2aa1lcohsfilglinatscetraatnwidaonsg,reaovsnasdluetaxhtteeendmaatlwsipcahecyhsdfiaccialely slOaanccrtaidtfaiioycne,d4ablolefcpaluuapcssteatwioeofrn,emocsraaiccbrhuifncidrcioetsdysv-wifaeosrdteeeceeadpxiatlmiaitteniroendwaafsnodrcueglxrloaesmdsitnloee'dsSiofmnosarlgaernsodsasnfodlres5itohnefse.mcaaluPessue.psofOfonduenaddtahydeo2ar1dtooheff ctmoiolmrleiepbcotuienndtds2c-o(6nodrihtoituohrne.s FpOTohsgteednoleusrniagntsig,o;nl,ifvoebrrl,otoahedn,dFmIainlygke,gnreaornsadstiloleinsvieoorfnfsssapwmrepirnlege,scobwlleloerocedtecdwoalflsreocmctoelsdleelocentcetdeLdDopnu1pL4s:Damti1lv4ak)riwaoanusds msparatemesprclnreavsledorfafosrbalponoodsds,pibulmpeislf(kubtl(uorioendcalanuandldiynslgiisv.etrheLsiavmmeiplrlskea-ssmepwclereertseiwnpegoroeglleadenpvdeaslr)u,laitatenedrd)vilaaitvvcealrreicwtoerurosneimmcioeclprlooeicsntcetodsp.y.fforroIamnnaasdledylisettcistooend,f PFOS concentration. Only the analysis ofthe sera samples are available at this te RESULTS M1Te6oameng/mrkaegts/peodmnaasylefwbeeofrdfeeycrtwesediubgcyhetddcochsoaemnlpgeeavsreleid-thtmoatcteorrnetnaratolel:dsgdrMuoeruaipnnwemparrteeemcranotamilpnagbroaadnbyldewceotoingtthihntousaienngdsetbehnordoiynugcwhoeoniutgtrhotglesgatanaiitnmisoanl.ast irdmueprdliuancngetdalaticintoatntriesoani.ttesd,Daduneriliimnvagelrstehadesspiczroeesmm,paaatrniedndglipoveerciioontdte,rroalssni.dzeoRwneedriunectotoibgosenessrtavitenidogniensattnartdeiaoltnaecdlteaatnnigiotmnha,,lsft.oheodavceornasguempntuimobnerwaosf o"fTroospxtirecreesdruegmsreopduopncsa.snenliObenacltilzsaecdtb.aytidoPnousdpeaymloserv2te-al4l,-itadypepwvraeoslxoiapmlmasetoneotlbaysle:1r9v%PeoudfptinmhaegrrtpoauulpptsyAiwnaatgsroourbapsteeCrovwfeed9r%ein.etiwtIhoneraodffdoittuhineodnc,rdoetsahsde- 24 ENVAMRD(2002)1 FINAL aanlnusdmobr1ee.rd1u%oc,fedrleisivpeneptchtuiepvsseelotyn.wodgRareyoduu4pcsto.filoancPstuapitnimoponru,tpanlbiuotmdybiyerwsgerioogfuhptsssuBrwveiavrinendgoDdbpsuuerprsivenpdegrilniacegtrra,otiuopansnddAafyiasvne2d-l4iCtweroenrseidzaeasy w1|.e6ro%ef l(lanaccettgaaattitioionvn.edcaoPnyutrp4o-l2b)1o,,dwypituwhpetibhgoehdtrysedwiuencitggirhotonssupgirnDeagtrweoseturpeisncgAo,rmopCua,praCab.nldeTDwoowcelorinetterrroseldsiundcguerrdoiunwpgheAtnhaatcnodsmaapmnaeercpidetreitorod.ognrogFruropuopBms d7i%donfotthenuprusep.s fMoiulnkdadneaaldysainsodfntehcerosptiocmdacihnsgorfopuupspsA,fCo,unadndno,mirleskpeicntitvheelys.tomachsof $7%, 100%, and fErleocmtrdoanmmsictrreoastceodpwiicthex1a.m6imnga/tkiogn/doafythPeFOlSi.ver revealed an increas in the number of peroxisomes in pups aeSstntadtaitpsietorincc/ealnlatcartgeaestsui.lotns:SatanTtdihsetciocmmaelptashrioegdndiofbfiycsatndacoiesstewicaaaslndanonbtaylaysscsiresossscseo-dnf.soisstTteherdeedodfgartcoaaulpwc.eulraetLiipotrneoersfenvataevldeureasacgcweoserr(deimneugastneodd+aw5hy.e0r.oe)f appropriate. The data aretoo cumbersome to cite here. seRchehrmeoadmuorlrkehsins--oarcreAihdfeidacie,t.isocSnaialglynsitaniolf,foracmblariatnsiicioaonln ttooonxiatcdhieetqyuhaeotabeds,leyrnveaecsdks,eisnasiltthheaendddoaartmas:fodrueArlliilnmbgm,attmhicesmsasiltnug,draytbsrpoeskrueironvdisvne(ddieo(r0 simnicsealtihgenyedalisnocioscorcsu,rraenddinltohcealcioznetdroalloapneicmiaal,s.amAolnlgproetghnearn)t awneirmealnsodtelciovnesriedderievde tooffsbpertinrge.atmentrelied Frinaotliplousopwsai.nndgbuttcherwolesarcsetfatoniosottnecironindosneinxgdewLreerDde 1tc,oomlbipevaertalribetlaeetrmaseminzoternseglwtaeerldegrscoiounmpcspe.atrhaSebiylgenaslobseoftwcolceicenunircratelrdetaotixenidcgiartnoyduwpecorBnetr(oonbles.geartSvieevxde cfoonltlroowli)nag etxhaemisnaamteiorantoe.f pNuopsliugnngsi.ficant differences were observed between group B and the other groups w1P0eR5rO.eS34bceuolgno/cmweln.tthreaSteliiromunistmioPnfFtdheOetescceotrinuocnmeo.ntfrSuanettirrouenmastPienFdtOdhSeampcsuopnrscaenfngrterodamtfuirnotonrsmeabitneeldtohwdeaptmhusep,slfimofisrttoeomrfedtdrewetiaettcehtdiuodnntarm(es0a.,t0e5fdousdtgaemhrse),d wdiatmhsurnainrgeeatdefdrdoamms59,.r2aungg/emdlftroom15477.u6gu/mlg. fostered with treated dams, ranged from Se0ru5m9.2PFuOgS/mclo.nPceRnOtrSatcioonncsenintrtahetipounpsinftrhoemsuenrturmeoatfedtdraantse,d below the limit of detection to 35.7 ug/ml. Serum PFOS cuomnlce.ntrTahteisoensdaitnathiendpiucaptsefthraotmetrxatpedod1sa0musP,rFOfeoSstcearnedowcictuhr btraetahteidn dutaemrso,arnadngveiadmifrlokmfr7o9m.5turematled td0an9n6..9 O CONCLUSIONS e ell J Pntheuegpasstiafvmreeocmolncotowrnotm)ro.orltMadolraittmayslirttahyattra(wt1ee.sr1ei%n)ctoahssesrp-efumopassienfrirenodgmwticwtoohntgrProFoluOpSds-,atmrhsaotwceredovsesdr-af(moisset.e(rpceoodnsttwrnioatlthadlacomenxstprwooisltuhdraeimtosnels(y1)f.r6ho%am:d drPeaFsmOpsSe,c-ttirive.e.al.yt.cbdotdUhanmdisen,ruittche.eroicnoanundtdietripooonsestxnpoaoftsautlrheeexospntolusydy;u,rea)tnh,disPhalFidOmSi-htietgdrheedaraetdamodsrpaaplmeissttywittroahtielnsidtieacrtast9ef.r6tohma%tPrFaeOnddSucer1d9e.s2pt5ue%pd, d`scuuorrnvijinuvgnaclltaiciotsnatmwiaoiintnhlloyinnea,uttrehersrouoleutxgphoofmsiuilrnkeurtmoearmyoeaexlxpspooossceuodnrtedraim0bsu,tdePoFtcoOsSrneodatuncdaedtphpaptutpepooshsuaartvvnriaevtaala.lnyeaIxdnpvocesorunstrereaasfvtfi,eacetxmpoionlskuprueip.n vfieambailliety.ratsAddeixtpioosnaeldlyd,urainnaglystihse oifn-PiRfeOSphcaosneceontfratthieonstsudhyo.wedAdtdhiattioPnFalOlSy,waPsFOobSsewravesdoibnstehrevesdesinofFseOra 52s ENVIMRD(2002)1 FINAL esanmcprlaetsiontapkuenpsferxopmosFe1d vgiaenlearcattaitioonn,pbuuptsnoftreoxmpofseemdalien erartsoexposed fo the test substance, and in FI These ae the conclusionsofthestudy authors and tis reviewer agrees. D REFERENCE ovel, nC1eh9.r9i9s.tPiOraronat,locM(o.SGl.a,vNauHgmeob)beeCrrrmo4as1ns8,-.F0A1o4s.,tMe,SipnoagnnsSdotruY:doyr3ko,Mf RPC.FoGrO.p1So9r9ia9ntcRe.atTso,TxeisctoilnoggyFa,ciSlittuy:dyANrugmubseRres1e.a6r2c9h5L1a5b,orJautloyrie3s3,, Sa3npMdraEgCnuovenic-reDonantwmrleaentytiaoRlnatLosafbEorxPapetroofsrleyu.dortoo1o9cP9t9oa.tnaessAsunilauflmoyntPiaectraefll(uLPoarFbooOorSca)ttaon(rcyCsuARlSefpoonNrauttmeobnevirta:hGea2Dv7ea5tg9ee.r,3m9iL.na3ab)toiroianntooftrhytehReeSpPeorrreutsmeNnooc.ef si2g7n7i9ng, 108 (R2e/q11u/e0s0t)o.r SPraomjpelcteanNoa.lys3iMs cToomxpl6e2t9i5o.1n3.JuneSt2u8d,y19i9n9i.tiaStitoundydaItdee:ntJiufniecat1i0o,n N19u9m9b.er:ComFpAlCetTioTnoaxt 526 ENVAMRD(2002)1 FINAL REPEAT DOSE DATA "PToittlaesssi1u0m4S-aWlete(kPRDOieSt;arTy-6C2h9r5o)niicn TRoaxtiscity and Carcinogenicity Study with Perluorooctane Sulfonic Acid TTSTESTSSUUpBSSTTAANNCCEE Hdenity: Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) iRmepmurairtkises: oTfthheetettestsusbusbtsatnacnec,eLwoetrNeon.ot21i7n,diicaateldighitntchoelomraedi,n fbreeofoldfowyihnegsptouwddyerre,poartn,di METHOD. eeeect Method/guideine followed: Guideline number not stad 86.9% pure. The LL) Study duration: Twoyears GLP (VIN): Ves earstudyperformed: 19982000 Species/strain: Sprague-Dawley rat [CF:COBS" CD(SD)BR) Sex: Male and female Nlouwm,bmeirdoe,faanndimmailds-pheigrhdodosseeggrroouupp:s cTohnetaiconnetdr6ol0arnadthsi.gh-Adohsiegghr-oduopsse rcoenctoavienreydg7r0ourpacto'ntsaianneddt4h0.e Routeof administration: Dict Doses testedand frequency: C(oGnrtoruopl3()G:r2oupppm1:): m0idp-phmi;ghldoows-edo(sGero(uGpro4)u:p 2)p:p0m.5;phpigmh-dmoised(dGorsoe:up 5) 20 ppm. Posttreatment observation period: Aafehrig$h2-dwoeseekrseocfotvreeraytmgernotu,p (Group 6) was observedfor52 weeks hoSfteattevirasortgiieacnnaeclietmy[eotAfhNovOdaVrsiAa]unsceewdaa:tsPL<ue0sv.ee0dn5e,'tostraatnensastlfyowzraemastbiododonynsewweetroieghtuetssste,dfbotorodsvytaarbwiielaiinzgcehetthhecohmvaoangrgeieannsec,iet.yf.oOondcIn-cvotnahsyeuamcnpaatslieyosnoi,sf u`wcsaoesndtdifnoounroeupsaoincrlwitinhsieecahcloopmmapotaghreoinlscoogonyussvbaoelrtuewtser,eanpnastlfrmoairttmeoedyd]adCnatdoa.AcoInoftxtoihdleagsAreoNauOcptsVi.vAitGirweoasusapnSdcigonomirpfgiaacrnaintsw,ieioDgnuhnwtneedrtoetta'.esvatlAeusNattOewdVaaAst the 5.0%, two-tailed probability level, wRwaeersmearrrekemcsoo:vredTedhdefaarsnoimtmhaeitlysswcweaergreeeoabonsbdesreverexvdae.mditAnwleidcl;eeaasdtbaniolonyrcm(aa. lprmi.foiranndtidongtpsraemoa)rtfmaeonnrtmioanrndtdiacalwtieiteoyknlaoynfdtnhmeoorrremiaahbleurnw,daiCsto;crhefciaonnrddiiemndag.ls Body weight data were collected weekly through Week 17, once every 4 weeks thereafer, and at Week Fy ENVAMRD(2002)17FINAL ct1hh0ee5r.meiasfFttreoyro.,d DucruoirnniasnluygmsipWst,eieoaknnsddau4tr,ain1ew4,ecrh2ee7m,ciosaltnlredyctt5ee3ds,tswbefleorkooldmya1fn0odranutirhimenaelfisrw'setsree16icnowlGelreeockutsepdsanf1odrthohrneocmueagtheovl5.eorgByy,l4ocoldwienwiecakassl cd1ou0l6r.liencBgtleWodoedfeokrficl1hm0os5lew(setWreeereoalklsao1n0dp3retfrpoiargrlTeydecemfroiardseandiienmtGaerlrmsoiuantpatt3ih)oenatsnedrfmrtiohnemalarlealcnodavneriremycaolsvsaecrrpiyrfiisocarecrt(iofGirtchoee.uptFe6im)viednuaarlniinsmgaaclWrsiefsiekcxe sminaemaGpsrluoerusepmsewnet1rsethbcryoolpulrgeohclti$efderwaeftroireon PscaeFclrOliSfniuccaelndeaaldryusraiinsnt,iggmeWineto(ecPkhCoNn4A;dr)il,iavlaernsdscwdieevrtieeyr,mcionlhaleetpciattoeondceolaflnpudalalwrmeiitpgrohoyelldi.-fCero[aAtTihooennidTravoseteer ahicemtpmiavntiotoyhc.eilslItnuolacadrhdeipmtriioolsnit,freyrlaiavtteiroWnseareamtkpel5em3se]awasenurdreepmcaeollnmltietc[tobeyydl-fPCoCroANPAoFxOiaSdtaWsaeneaedlkeystie1sr4m(iaWnnaedteikbosny (1bW4r,eoem3ok3,d4ea1o0nx3yd,ur11i40d)5i,naend(B1r0d6U)), RESULTS iShnuecrrrveeiavswaelassrianatesssui:rgvniivfailcainnt miindc-rheaisgehd(t5r.e0ndppimn)suarnvdivhaligthh-adtooscec(u2r0r.e0d pinpmt)hegmroaulpss tahsatcowmapsardeude ttoo tshiagtnioffictahnet cisnuornvttihvoeallm.igdrN-ooudpos.siegnN(iof2in.c0eanptopftmr)tehngedroowtushpenraontrdeesntoitendisnugrrtvhoieuvpamslidiin-nhfitegmhhael(em5sa..l0eTpshpemrre)evweaaanlsdedahisaginhgy-ndifosisiceganni(tf2id0c.ea0cnrtpeoadsrief)fiegnrresonuucrpevssivaail3n compared 10 that ofthe control Neoplaste effects: aTnhdemreasmulmtasroyf tghleansdtsu.dy show that POS is carcinogenic, inducing tumors of the liver, and of the thyroid 2s ENVAMRDQU) FINAL a -- TY-- Toos t Te S |Te] ioc | te | adie | aw |o7e|wrme |] Pvhoecne| || Cocntoomsm G5sa8o |log | | a032 am | | ws3ww db | | 5 wo | do52b | d% e | as7s || driy | aB7iae 20 er won | ain | 6| Fone dem | dh | dom i HSeoame||| Coantm | oto o0)t | 2a0e of | | 2d0sm em | | o0sh0 || at | 5 So wo SgWee dhee sTfoaedraion || win ai6o | do _| el0oo | db oosw | | ie dw3l | , d7ise CoGntde | Sims vaty | acSoi | | oeJmw || ooSnwh | | ed7dw CPanwitoner| Combine lw3e || aaiSl | OwFveree || a Sew | awIsbT ||I d|Ws) | dsien | daae || asxe ddeon +S + Sinic cpaoiina ocvwraeendoaFv|t s2re0thd0e)Gcoentmrol|(P< Gi 0.05. | dba | dem # Recovery group: aller 5 weeksofemer. dcRAoLeimmfepaafrrrkweesid:fntooomafnttihmhealscstoundtgirioevlses,.nmteFhaonocdobnoctdrnoysluwmemipagihrotsnloffeotxrchemepatsleufsrriatnnhdegfhmeimagahll-sedsoaswnedamsfeslmsmelenswwhieinrceh4nhootdsnsigdiafitpcaeondssly ToTSfligeOonriUmafticeaagnbrtylocyuoth.melphotawhireeerrgdefho1w0o-edrtdehcgaootnrnoososfuusetmih.pgntiicfooinncatnodtlu.rdienTcghreewraesefeeosrsei,n3 shuaoprupaegalhrsin4t.hnryFHiaigcheeetrrdfmeoanlteve3e0msd oSeoluteBhosuynedn Nomncoplasc effects: NOAEL LOAEL dose an eect: (daond sstee: 02.popormms(F(lelema)ele 5pom Female Hcepoooonipciy.epacohacryacitcerigserdambsy, scigoalnoincrheeaspewsociynccepniigombeuntlarotbyspeosomphey,s croelopuor 2 ENVIMRDGOO FNAL 0iBane5ccp.urse2ota.los5ecass,ol0ininrat2rwh0aecspeipnnnmcioi)tod:eebdnhucoleiwnreovfmpeacrl,yeetieasonhdlpoeerpwoefaeiscmoaealneiuiosrdboseblogsgavimnvdeaedni0oiSnnbdeowradoos2ct0oeoopopnkm(3.4gppA)fhspeieglnmienfeicasniteneseeeosmestin opfcbeoomsmmaieldienerrrfvesdertrhoeesb.elofIweaocmbtesnveterrcelld(t-NeddOv.AeErLsT)-heefrTottrorPleR,vOeSba(1aLsdOcoAonEsnLi)dtehresepdce1otnh.inoe0pd5clphomeengephoesn e orseere o yoo ResofuStalisitcl Ansys ofNoweopLesuiosns tinMiisce Rats, Go | Com7et | LoT w | ni3a tiGen | Groupss iver Vacuolaton, Hpsoseoi NidzomICrreiotair Recmery | "vas 0/65 [oss T6iss 0.0060+++ [p-ivvaelrueRsyTpa0ru0op0hy0,: HTeNpAato1l.C16o9 t|s0024+%+|00otse+| tsar | ivvaelrue-s.Gralar Cytopla2e5,5Eosi[na4pi5lcs, CeTr2i200s50e05++%Ta120/05006+5++[340 | 0000-++ [Toes Toss loss Toss" Trae Tom Toor [pvaluesT0000 TNA NA IBA [oto [Na] [oes Toss Toss oss NA 6/65 0139+% ie [5/65 Taiss Teiss [si55 Tiaies Jamo | p-values eT mvt 0 ENVIMRDGOFIGNA)L ResultsofSisticalAnalysesofNonnsopasieLesions in he Female Rts on ComCm lEow TaEGvidi ero es metoWewiec wNNs wR s Wass ive bres peony, sooToceair, CCoEnl|aBnsone tgues r biveersrtCeam,E osvhei,SE ncomin maenee cr ign, we Hepo W a,Cw oN non wM s Mus tehonTeya| "sRE wotn | mSeo | osmose| w3o | aon | | tuoes | ee o30o0sn oon. aerNs esviddeTeaEteo, a gs aw ssess bcaseesDegen, CuoGtewese W ws Wows dase omiseos || aa04 | owe du| y:m etres perp. HeSWpoaae,Perpe er mes aSse | awds | we bieersnts,McetoaWgEmT,ePignered, Na deeesoe | me| m" e m ENVAMRD(2002)17FINAL Lpis<to0f0s5ta:tistically different non-neoplastic effects (increased compared with controls, Maies (0.5ppm: Males 2Lipvpemr:- Degeneration, Cystic LLiivveerr-- DHeygpeenretrraotpihoyn,,HCeypsattioccellular Centrilobular Males (5Lpipvme)s-: Degeneration, Cystic LLiivveer-r- VHaycpueorltartoipohny,,HHeeppaattoocceelllluullaarrMCiendtziloonbu/lCaerntilobular Males 2L0ipvpem-r):Degeneration, Cystic LLiivveerr-- HVaycpueorltartoipohny,,HHeeppaattoocceelllluullaarrMCiednztroinlaolbu/lCaerntilobular LLiivveer-r- PGriagnmuelnatrHCeyptaotopclealslmu,laEro,siCneonprhiilloibc,ulCaerntrilobular FemalesL0i.v5ep-rpmN)e:crosis, Individual Hepatocyte Females N(2onpepm: Females N5onppem): LLiivveer-r- GHryapneurltraorpChyyt,opHelpaastmo,ceElolsuilnaorphCielnitcr,iCleonbturlialrobular FemalesLi2v0erp-pmI)n:filrate, Macrophage, Pigmented LLiivvee-r-r HGyrpaenrutlraorpChyyt,opHelpaastmo,ceElolsuilnaorphCielnitci,lCoebnutlrairlobular LLiivvee-rr- PIingfmleantte,HeMpaatcorcoeplhlauglaer,,PCiegnmterinltoebdular LLiivveer-r- NIencfriotasties,, LIynmdipvhiodhuiasltiHoecpyattoiccyte. Liver- Hypertrophy, Hepatocellular, Perportal Remarks: o"bTebhseierrecyvaeettmdiecnitnd.ermgeiledan-teedrdoa.steioH(no2wopefvptemhr)e,lmiAavselireginsriabfse.ilciaTenhvteerdienftcoorrebeae,sedthuieen Nh10eOpoAaltdEoaLcgeeflooufrlatthhceeenmatanrliielmoabrualtlaairnsdhcyoipsnesnriotdtreocrpoehndysitwodaesbraeeds0.to5o ppm. agree). The author concluded that the NOAEL for both male and female rts are 2 ppm (which we do not io"Trnhcberyreeabswerasosmionndoceealelxffypeurcrotliiodfinenrhaeet(ipoaBntridacUsp)maaeltamsiwuteroeeykdl-3bC.yoAprooxliifdearsaetiavceticvietly.nuTchleeraer wanetriegeanls(onPoCsNtAa)tisattiwcaelleyk4ssignainfdica1n9t, m [l=] TomTo [omJorfry TThomes [aces5 | vegeo50| vee150|oe150] eenssom] 0 [ona[055000663|Tors02m7 | sesame | zen Sorin east,fma) Traeaslls | ewsomanl|l mIalin| easelsl| merald E E EE m O T ui e c Lr rT ntee e9514870 a En Ed i ERC E I e Ea iREen hoy 0923.41.77 604180 =m Westies FE TeTa 0185 +0184 1294681 BIe6l4 tE I 2o5p INNES S stIe EIEA EA 3 ENVIMRD(2002)17FINAL s(TiPhgeCnriNfeAic)waanostrinbnoycrbeefrafoseemcsotdioenoncseyhleuprpairtodilicinfepera(alBtmriidotnUo)ay.sl-mCeoaAsuorxeiddabsyepraoctliivfietrya.tiTvheecrlel wneucrleeaarlaontniogesntatistically 5. ptPhoFetOacsSosnitwuoamlssaaonlbims(aePlrsFv.eOdS Ti-n62t9h5e).seTrruamcealnedvellsivoerfPofOSratswedroeseodftewnitdhetepcetrefdluionrothoectsaenreumsualnfodnilcivearciodf REFERENCE eee eee 3AVMco.ild(u2mP0eo0st2a)1s.-sIiX1u0,m44-0SaW6le8tep(kaPgDFeiOscS,t;aJraTyn-uC6ah2rr9yo5n2),ici2n0T0oR2xa.itcs3.iMtFy,ianSan.ldPRCaeaupro,critMn,iongne3MneiscoTit-tay6.2S9t5ad(yCowivtahncPeersftluudoyronooc:tan6e32S9u-l1f8o3n)i,c Sl2ie0fa0ec2tairm,e AdiMct,aryTehomxfoprdot,oPsp.oJtu,asarsniduemButpcerafhloufofr,ooJc.tL.aneTseurlmfionnaatle.obsTeorSxvicactoiilo/nsTion xSpiracgouel-Doagwl6ie6ys(1tr-a9,s) a1f8t5e,r as ENVAMRD(2002)17FINAL EPIDEMIOLOGIC DATA. "Title: Identificationof Fuorochemicals in Sera of American Red Cross Adult Blood Donors: TEST SUBSTANCE Identity: PROS and 6otherfluorochemicals Remarks: The results reported are preliminary. The fnal report is expected November 2001 Ne ree LL Study design: Cross-sectional ManufacturingProcessingUse: NIA 6HyrpeogtihonessiosfttheestUedS:. To determine the levels ofPFOS in the serumofAmerican Red Cross blood banks in Study period: 2000 Setting: N/A A"Tnogteallesp,opCuAla;tiMoinn:neSapeorluim'SptooiPcadulf,rMoNm ;6 CAhRarClotbtleo,odNCb;anBkosstinonv,arMiAou;s Pgoerotgarnadp,hiOcRr.egainodnsHiangetrhestUoSw:n, LMoDs. Subject selection criteria: Unknown "Total#ofsubjiencsttudsy: 652donors,age 20-69 years Comparison population: N/A Participation rate: N/A Subject description: Noinfomation was provided on he individ rom hm the sea spl wre Health effects studied:PFOSlevels in blood Data collection methods: cBhlrooomdatsoergaraspahmypleelsecwterorsepraanyaltyazneddeumsimnagshsigshp-epcrtersosmuerteryli(quHiPdLC/ESMSMS) Details on data collection: No information was provided as to how the blood was drawn, stored, ctc. Exposure period: Unknown--PFOS serum levels used as surogateforexposure. Description/delineationofexposure groups/eategories: N/A Measured or estimated exposure: N/A 536 ENVAMRD(2002)17FINAL Exposure levels: N/A Statistical methods: Acaarnlidctuthlhmaeteietrdi.rcesmCpeeeancnttrsiav,leirntatenengrdeasec,nticgoyneoatmneedtmrdsiiswctirmilbeuatbniesondoaofnntdehi9en5td%haetcafobinnyfaiadrgeeenp,ocregt.einndtAeerr,rvaelllosacbawitelirioyen assessment is also being analyzed. Other methodological information: N/A =RE. SULTS sDresacirfiebdebryeasgues,:geTnhdeer,meaannd gseeorgurmapPhFiOcSlolceavteilonwawisll43b.e7fpobr.thcTohmienrgainngethweaisn4a.2r7eptort1656 pb. Aralyses pSinrteultdihymeisngtaerrneyenrrgaetlphorspt.oapnudTlahwteieoasnka.nmepslseNeossi:zoethserreTldahetseicsvreeilpydtaistvmaealalienfcorromsas-tsieocntiaobnoalutdatthaeusseudbjteoctdsetiesrmaivnaeilPabFlOe Sinlevtehlss Blood donors cannotbeconsidered representativeof the general population ofthe US, Research sponsors: 3M Medical Department, Corporate Occupational Medicine `pCooonlseidstbelnocoyd osfamrpelseusl,tsa:ltMhoeuagnh tPhFeyOSareleovenltsh rehpiogrhteerd einndtohifstseturdaynagree similar to those reported ear in CONCLUSIONS - NA Re EFERENCE ee ll Oflusoerno.cheGmWi,calBsuirnisse,raoJMf,AmLeurnidcbaenrgR.edJKC,rosHsanasduelnt,blKoJo,d dMoannodrse.l,InJtHe,rimZorbepeolr,t. LJRu.ne 2I5d,e2n0i0fi1cation of wm ENVIMRD(2002)17FINAL EPIDEMIOLOGIC DATA E"TmiplleosyeAens,Ep1i9d9e3m-i1o9l9o5gic Analysis ofEpisodesofCareof 3M Decatur Chemical and Film Plant TEST SUBSTANCE erredbergmretttoe ltt LLL Identity: POSF-based chemicalsusedat the Decatur plant Remarks: Episodes ofare analyarseneost ofin used in occupational epidemiologic suis. METHOD Study design: Episodeofcare comparison puMrpaontouecfftaitcvhteeurcfiihnlegmm/iPpclraalonsct,esapsneidrnfgto/hrUemsaecn:hceTemhicceahle3miMpclaanDltes.c,atauTnrhd,eAf3lluamobraaojmeolaraspptlroamonedtrusbc.etgagnroPpeurprfsolduiounrcottohcietioanncchse1u9ml6i1fc.oanly!11plfialsnutmoardiadere.e p(rPoOdSuFc)tisontohfeamavajroiretsyuolffopneartfelufolruionraotcehdeammiicdaels,maanlucfoahcotlu,readcraytlaDteesc,ataunrdaontdherisfuusoerdocahetmhiedparlepcuorlsyomrerteo the wHiytphotlhoengs-itsertme,stheidg:h eTxopoussuereeptiosoPdOeSsF-ofbacsareed pmreotdhuocdtoiloongayt tashea3sMcrfeaecnilfiotry imnorDbeicdaitutry,oAultscboamrems. associated DwSeterucedeymabnpeaerlryi3zo1ed,d:1f9orE95pw.iosrokdeerssoaftctahreepleaxnptewrhieoncweeorfe6e5m2plocyheemdfiocarlatemlpalstoy1eeyseaarnbdet6w5e9enfiJlamnupalranytI.em1p9l9o3yaeens Setting: 3M plant in Decatur, Alabama, hToetatlotpaolpwuolratkieornp:opu1l3a1t1iownorwkaesrsnowerreepocrltiedg.ibl forthe cohort (at least | yearofemployment at the plant). HS1iu9rb9ejd3e,actntdesDemelmcec.itin3o1an,o1tcr9red9it8die.r,diadE:upriAislnolgdewtsohoerfksetcruasdryeewmpeeprrlieoody.leimdiHtaoetwdtehvteoertDh,eecieractDouerrcdpasltauonfretmtfipomlreooatyfeleeemassptloon| yyMmeeeadnritbcfeaotrrweeeelmnopnlJgoanty,eere1ms, odifscaabrileitfyoorthwatheompclhooysees.HMO coverage were not in the database and would not be included i the episodes. ecCxoopmmoppsaaurrreeids0o0nPeaOpcSohpFoutlhMaeutro.irooncE:hmepmliCochyaeelmeipccraooldmupcaatrniiodns.ofnilGgmrroopuulpapsntAw:eermeaplldleocfyhieenmeeisdcaawlecrpleacnatneosfmloyprtzleoedydieseipesopatanrenandttgeially wiaonrmkdppltlahacneten ccehhmeepmmliioccyaaellesppllaealnnittgieabmnlpedflooalyrleeftishlemwcipothlhoarnhtt.igehmGpfrlloouyuoerpoecsBh:ewmhiacollalwcoehrxekpmoeiscduarleexspcllaucnsotimvpeeamlrypelidonytetoheesthfweirlhmjopwblaoncrto.kuendtGersrooplualpnysCi1inh tahlle the sfitlumdyploannst.et GcroomupaprDe:d 0altlhaenitjowborckoeurntserwpiatrhtshiignhthflufoirlomcplhaenmti,cal exposure for at east 10 years prior to the Participation rate: 97% ofDecaturemployees were eligibleforparicipation in the study. 8 ENVIMRD(002) 7FINAL ttShhueebjcfehcmetmidpcleaaslnc)rp.ilpatnTithomen:pmle8oa2yne%oeasgfethhwdeasewmo4pr5l.ko1eiydeeotsnh yincitnhheetmhcieochcaohlretpmliwacenartleapnmldaanl4te5a.(65n3ida0n itsnhiemtihfleiacrmhpepelmraicnctea.nltapSglieaxntotyfHapnemdrc5ep5nlt8anoitnf whiogrhkeerxspohsaudrewojorbks,ed exclusively in the film plant. Seventy-six percent ofthe chemical plan workers hod dcHeiersatolaritdnhereespfifiesncovtdoselssvtiounfdgicetadhr:ee tMwhoeyrrrbeoiididctoyng.sliadnBedaresadenddaoplnirpiaiondri.mmeatlTadhbaeotlyaisnimnd,cluegdpaesidtd:reomiinlotielesotrgiinacanldstdubidlsiaoedrsddoeerrnscPaoFfn1cOehAre.aenTdnudePsnEnOaSnn,: biliary tract, and reproductive disorders. vsDeaarstviascecesod,ll0aecntdpiopnrremsectroahipcotodvimsop:nriedThrhuedegnssCileuivnseiecdgalrionCuaptrihenegGdorifoaauglpnlossviiesspi,itsstoirdneepaatotmfieecnantrteaansndodftomwwaapnraaetgdeeenmvtee,nltoppreoodfcebmdyourrIeen,gtaehnnacn,ill4Ian0cr.0y d`bciyosnesdaeisaterisocnhoiornngcotbnhdaeictnkiwodnaser.draeTcnhodredf.soorfwTtahwreadrieinndcetoxidmereeccfooonrrsdttrchuoecnthsseiasalttshoefcpeliiastiohmdeserreopcfcrooarcrdesedutarhreaotcuonarddeetsrheeilnaidtniedcdeaxt0i-vcelthiogifabdliesfearacesocetooor-rd face encountoerar pharmacy recordfor delineating drug, JEaxn.po1s, ur1e 9petro9iDoed3c:. T3h1e, e1p9i98s.odesofcarethatwereincluded inthe study were those experienced between wpDoehtsoecnrthiiaapldtiwhooinrgdkheplleiaxncpeeoasteuixropenosjouofrbeses,x:paonwsdourrtkeheorgssreowuWphhso/ocwawteoergreokreeimdepsla:toylWeeodasrtskoel1re0slyyweieanrretshpeilncajhcoeebmdsiicnwatilotohgrrhofiuigplhsm ppaolctacenontrts,diailhnogfstoeor fluorochemical exposure. `Measured or estimated exposure: estimated basedonjob history information. dEexspcorisputrieon levels: Not measured. Employees were placed ino exposure categories based on job oSetpbaistseiorsdvteeiscdoal0fcmeaexrtpeheocadtmesod:negpAifsiorldimsekspolrafanttcoeamerpplfioosyoedecesh.eomfiTccahalreepel(xapRnetRcEetpmeCpdl)nouypmreobevseircdoeofdmcptpahireseodedscttsioomtahfteceaoorbfseeirfsvokedbbotetothewxtehpeeencfttiehmde aaindnjdtuhscetheeUd.mSir.catailBoepwclaaausnstceealmtcphullocayhteeedmeisacnwadlascaoncmadplcafuirllemadtpeltdaonfthreocomuhnhoaredtajstuhbsatcdeldasiliimsgskhtdlaayttoado.ifffeItrnheenmto3asMgtecmaaasnnedusf,gaetcnhtdeuersrisnskgirrpcaottpiuoursleaswt,eiraonen comparable. Therefore, 95% confidence intervals were only calculated for the unadjusted rik ratios eOfpatlihsseeordpeoosmifettichavoreedaonlcdoouglfidaclasrleepnrieengsfaeotnirtvmeaa)tn.iyonaI:nndaadIltdliistnhicooinudlednttybpceeassneaosn,tdepdrceovtuhaanlttensoftrfcoeamspeisas,noadenepdsi/odreomfmiicosalcrolcgaismcsaifypieerddsipcfefacestreisvbey(,botathnhe ssofeorfavtinwcaeerspeiusssuoecddhe,amaanadybivapirryopcoaesdmsuoirbnelgse astnohdafttpw2raerdseicfrfpierprtoeignortnasmdsim.aagynTonshoeets bcmleianyriecpabolertafelsedsfxiiogbinletidthyetooefpttihhseoedsea.amlgeAorlepioit,shomhdeem. aeynCdegpriotifaneitrn depending on the software program. RESULTS Describe results: The only increased risk of episodes for the conditions of a priori interest were for 3 ENVAMRD(002)17FINAL oCnveheoripalhlascimnascnlcoufedetcdhacetaenmgcaoelrreyofrfoerptarhloeldumcatclioevmepraesrpyrisostdoeunmctgairnvoedupssfy.osrtTetmh)he. orviTeshkrearlraletiwcoaawtseaagsnorgyrionecfartceaeanssecntdehriseskagonfd erbpoeinfsiooegdmnepsgulroooyrwpethehe.s (iwsiRtkRhEotRfhCeeph=iisg6oh.ed7se,ts9ai5nn%dloClnoIgn-g=teis1mte,ex-hp4io5sg5uh)re.exspItotssohuforlueolredbomcephleonmyoiteceeadslstahla(stRotRwhEeaPscCorne=fpiod1r.et6n,ecd9e5fio%nrtemCr1vaa=ll i1rs.e2vper+ordy3u.cw1t)i.idvee.Infcocrarnemcaaesjresed errpeeippsrroooddduueoccttfiipvvreeocscatananctceeerrcssaniancnedcrhtewhmaeiscsaoulbbs-peclraavtnteegdeomirnpyolfoifylpmereopslsat(na1tt.e8emecpxalpnoceecyrte.eeds),F(i3ov.f4e weehxppiiecschotde4de)sw.eorfcTeahrpsreofsitwnaedtrieencgaonibcseeirmrspo.oerdtOafnnoet bHeocwaeuvseer,antheexcuepsdsatieon fptrhoesastteudcyandciedrnmotorcaolniftiyrmwathsisofbisnedrivnegd in the Cotage Grove plant mortality study: g2Tr9ho,eurpe95(w%RaRsECalDnC=i=n1c.r17e8a=,se95d.52)r%.isCk1oMof=set1p.i2os-fo3d.te0hs)e afeonprdisntoehdoeepslloanwsgem-rsteeorafmttrehimebpugtlaaosbtylremoeitnnot,esbtheiinngiahglnerxcapootlsounirniectghperoolhyuippsg.h(ReRxSpEiompsiCulra~er numbers ofepisodes were reported in fim and chemical plant employees. ocnabtnshceerervee(nd1t.i5(r0ee.9xcpoehecoxtrpeted,c)to.endl)By,ot1bhoetpohicscioundrertehodeficcnhaferiemliwmcaapsllarnpetlapneotmr.ptleodyTefhoeersr.eivewOransclayna2cegcrrae(as0.te6esor ferxicspikechotofesdil)soawonefdrtIhuerfioinrvaerbyrlawtderdarecert eeimpnpifelscootydieeoesnssr.eipnorcItheedims.idciafflicpullatnttoemdprlaowyeaensy, bcuotnchleusyiownesreabmoousttltyhedsueeotboserrevcautriroinnsg,epgiisvoednesthoefcsamraelblynutmhbeesrameo.f =1`0Ch1se.em2eikcac5l.a5r)pe.lafnotEridegimhsptolereodnyereeseopsifsitonhdetehsoebifhliicagarhyreetxrapwcoetsruterheao,nbtslheoeirnrvgce-odtuenirtnmecerhpmeapmrlitocsyamlinepntlhtaengtfrieolmumpppllwoaeynrteee(sR2RaEndPiC1m.4<sin2m.6of,irle9m5lp%ilkaeCnl1ty cwhoorlkeercsy.sitiTsheansdubc-hcoalteclgiotrhiiacssisthwatitihnfclhureonnciecd occurred in chemical plant employees. otrhisunosbpseecrivfaiteidonchwoleerceysteiptiiss.odesMoosftchoolfeltihtehioasbisserwvietdh caacsuetse rdRioiausbpkest.reast,iCohosynpodefirtleiipopinissdoedwmehisaio,cfhacnwaderreoetfhoenrorteennidddoeocnctrriiifnnieeeddoiarsopnrruidioerirsts,obnuwathliwdchihiscoirhdnecerlxsuc,dleuwddeersdeubtn-hocetatmceulgleolvraihteyespdootifhnettshhiyesrcoiiondmtpdhaiersei9as5so0en%, Pcaonncfriedaesn,ceysiinste,rvaalndfolrowtehre uhriignharyexrpaotsuirnef,ecltioonngs-term employment group. included: disorders of the AeSpltitushdooyduegsshtrotefhnegcetaphrisesosadonfedtowfacwreaea.rkenedTsehsseeigsn:reasluSlleoseweo"dfoftothrheirasdismrteeutcdhtyodcosolhmoopguailrcdialsoonnlioynffobrwemoarutksieeordns"fwosiretchthiySopinomtilfhoaerrsidlseimmgioetgnaretriaaoptnhisionco.sf ddbiiufstfeiacdsuileftfei{rn0ecniitndteeenxrcppero;estutrbheeesrce,afuotsrheer,aetlhaairrsgeepmaRraRanmEyePtlCeirmmicataaytninnooontts nbteeocetishnsitaserridpleryseitigenndd.iciantEeapnihysiogdohetrshiesrokfomcafanirnneecria.dreencTreehopeofrdtdeaidsa,eaamsroect pwIanatsaedrndlsii.tmiiotneI,dnmdtia0vni6yduoyaeflasrtsh.meayrAislbkser,actotihuoesntfweoidrlcimpzoiarsteoodnetshoafonfhoecnaalcrteeh hicnaadrtehveesredyravtwiaicbdeasesemcoabnyefciardeuefsnleeccettihlnyotcearclvaalnmse.bdeiTcchaaleteapgnroaarlciyizsceeed tuinmdees.r lNaorgteraloremspmlalolyeeresdwieseraeseicnlcalsusidfeidcaitniotnhse.daEtpaibsaosdee,sosfcuaarcethmohaseyoinnclloundge-ttehremsdaimsaebiliintdyi.vidual several Research sponsors: 3M Company 340 ENVIMRD(2002)1 7FINAL Consistencyofresults: Noothermorbidity studies have been conducted on fluorochernicals COeNCvLUSeIONS wThhiosasrteudeympslhoouyleddoinnljyobbsewuisthdhfoighheyxppotohseusriestgoePneOrSaFt-iboansreedgafrlduirnogcwhoermikcearlsse.mployeda the Desstur plant REFERENCE - p2O0lis0es1n,osGWo,f cBaurreleowf,3MMM,DecHaotcukrincgh.emBiBc,alSkarnadt,fJiCl,m pBulrarnits,emJpMl,oyMeaensd,el1,99J3H-.199A8n.epFiidneamlioRleopogritc.anaMlaysyis1o8,f Ww ENVIMRD(002)1 FINAL EPIDEMIOLOGIC DATA "Tite: Idenificationof Fuorochemicals in SeaofChien in the United States TEST Se UBSTANCE eee tl 11 Identity: PROS and 6other luorochemicals Remarks: The results reported are preliminary. The final report is expected November 2001. METHOD. - Study design: Cross-section. ManufacturingProcessing Use: NIA eHhyiplodtrhenes0isprtoesvtiedde@ mTooredestpeercimfiinceutnhdeerssetraunmdincgonocfetnhteradtiisotnrsiboutfiosneolefcttheedseflcuoormopcohuenmidcsalins cihniladresna,mple of Swteurdeyanpaelryizoedd:inChSiplrdinsger1a99s9.amples were collected from January 1994 to March 1995. Th sera samples Setting: NIA "Total population: Not reported iDSnuefbpejacertcitotmnessnetilneoccfhtiiloPdenrdeinca.rtirtiec`srT.ihae:cThihTlehdyerenwseewrreaeresoabrmetpsaliiednesendtwseforrfeom2p3raosvtlaiatdreegsedinctlotihn3eicMaUlS.bryiaTlthheeosneUnGcihrvioelrudsrpietnAyproseftsreMeniptnteondceoswcoicttaahl signs and symptomsofacute-onset pharyngitis.Al ofthe children had positive throat cultures atthe intial "Total#ofsubjects in study: n= 599 children, age 2-12 years `Comparison population: N/A Participation rate: N/A t`aSkuebnj.ect description: No information was provided on the hildeen from whom the sera samples were. Health effects studied PFOS serum levels in blood, as wel as 6 ther luorochemicals. cDhartoamatcooglrlaepcthiyo/nelemceitrhsoprdasy: tandBelmoomdasssesrpaectsraommpeltersy from equal numbersof male and female children residing (HwPerLeC/EcSolMlSeMctSe)d, in 23 states. uTsihneg sahmipglhe-sprweessruerecollleicqtueidd Details on data collection: No information was provided as to how the blood was drawn, stored, tc Exposure period: NA a ENVIMRDGOG2) FINAL cDheislcdrreinpt2ion1e2lyieunresaiaodn.ofexposure roupscatsgores: Bloodsersmpes were collected from Measured or estimated exposure: NA Exposure levels NA Scitnaattleirlsatecitdcia.oln cmCemteshnowdaisl:ltbeenAdderonnceeytiaetnhd mdseiaslnrsi,bpuroiaon.ngeosfA, tcaheobdmyeyrbysmeagaoen,sgbaenndder9sh5l%ooccaboteinnofnigdanenncehsneenraecwoerne Other methodslogica information: NA wReessuC LTs LL L tDsestciriiebdebryesaugle,t:genTdheer,maenadngePoRgOrSaphsiecroaalieovenl wwiallsb4e3.F5opcbo.miTnhgenrtanhgeeoways r6e7ce-.515 ppb. Ansyses "SUTSth.usdaychsmiltdrpeennls:gitezhNsiosaaenatdteiwvreealdkenscesrsmispaetlsi:veTihnefsoermdaatitonaraebocurtostshsecstuibojneacltsdaavsaetd ato ebstempeePlFOoSslewvelsmin Research sponsors: 3M Medial Depimen, Corporate Occupational Mine Consistencyofresus: To dae mo tee dt ave been collected on POS serum eels i hire. L cA oncc Lusions o SA Na A Oolrsoecn.hemGiWc.alBsurirniss,rIofMc,hiLludnrdebnergs,h JKU,nitHeadnsSteant,es.KJr, iMamndReelp,ortJ.H,J Zobe3l,, 2L0R0.. Kdentfication of ss ENVAMRD(002)1 FINAL EPIDEMIOLOGIC DATA "Tiles Morality StudyofWorkersEmployedatthe 3M Decatur Facility J TEST SUBSTTaANNCCEe entity: Several perfluorooctanesufony! luoride-based fluorochemicals, including PFOS Remarks: This study is an update ofthe study published by Mandel and Johnson, 1995, METHOD -- Study design: Retrospective cohort morality study. ttm) puMrapontuoeffctaitcvhteeurfciihnlegmm/iPpclraaolnsct,esaspniednrfgto/hrUemsaecn:hceemTihcceahle3mipMclaanDltes.c,atauTnrhd,eAf3lluamobarajomloaarsptploramonetdrusbc.etgagnroPpeurrpofsdluuiocnrtoitoohcneiaincnchse1u9ml6i1fc.oanly!Itplfiaslnutmoraaiddreee p(rPoOdSuFc)tiiosntohfeamavajroiretsyuolffpoenratfelufrliunoarotcehdemaimciadlesm,aanlucfoahcotlus,readcraytlaDteesc,ataunrdaontdhrisfulsueodroachetmhiecparlepcoulrysmoerrsto, the oHfyepmoptlhoesyiesesteosfttehd:e 3TMo dfaectilrimtyinien Dwehceatthuerr,oAclcaubpaatmiaonal exposure to luorochemicals is related to morality 3S1t196u,1d1.y99Tp7ehraesioctdoh:heoirTtahswetadssattufedoyloflepoomwppeuldloatythimroeonnutgwh,orDkece.d 3at1,t1h9e9p7l,antCufrorreanttllyeaestmp|loyeyaerdswionrcekeirtsbewgearne parsosdiugcnteidonDeicn Setting: 3M plant in Decatur, Alabama o"Tnoetayleapr.opuEliagthitoyn-:fou3r5p1e2rcweonrtkoefrtshweecroehoirdtentwiafisedm.aleO.f these workers, 2083 worked at the plantfor at least fSoulbljoewcetdstelhercotuigohn Dcerci.ter3i1a,: A19l97w.orkCuerrrsenetmlpyleomypeldoaytetdhewoDrekceartsurwpelraentasfosriagneedaDses| y3e1a,r,19T9h7eacsohthoeritr waasts date of employment. MCoormtpalairtiysroenferpeonpcuelartaitosn:froImn 7SMrRegioannaallycseosu,ntiMeisnnweesroetaalpsoopuulsaetdiotno dreulaethourtatlesargfeorvawrhiiatteisonswebraeseudseod.n regional morality reporting differences. Participation rate: Death certificates were obtained for 96% (n= 139)ofthe cohort who were deceased. e`Saurbsj.ecatnddetshcerimpetaionn:nu8m4be%orofftyheeaermspwloorykeeeds iant tthheecpolhaonrttwwaesre14.m9a.le.ThTehneummebaenroafgpeeratsofno-lyleowa-ruspawtafsol5l1o.1w yuopunwgaesr5t0h9a7n0.thTohteherre2wgerroeup1s45budtewatohrskeidde3ntyiefaierds linonthgeeacrtotohre.plaTnthehahnigthh eoxtphoesrur2eexgpraosuuprewagsroulipgshtly Health effects studied: Mortality yDeaatraecmopllleocytmieonntmewtehroedsab:strAacrteevdie1w0:roefcoermdplthoeyeweorwkoerrsk"hnisatmoer,y SreScNo,rd3s Mofiadnenytiefmicpaltoiyoneenuwmibtehra,t dlaaestof| 34 ENVIMRD(002)1 7FINAL c`bwimrotprhkl,eorsya.nmdenDditastcisrneofpfoarwnmocaitreiksonwhiiatsnthodrtyhv.eeroi`rfTiyhgiisontachloehrcoordhtaotrawt.awseTirhneekreeNdsaottolivroenedacloarnDddesadtefahrtohImsntdbheeexfoowrreaigsi1n9sa7el9awcreocbhroeerdtvfteoorriufapileddatonef ttthhheee Social Security Death Index. A licensed nosologist coded th death certifictoaItCeDs . Exposure period: The potential exposure period was from the plant opening in 1961 to Dec. 31, 1997, btDhaeesseccdrohiooprntti:oobni6d5heiilsnitontrehyaetihiniofgnohrmoeafxtpieooxnsp:uorsheuirgghreoeugxprp,oou2spu7sriiecn,attlheoegwooreiwxepseo:xsuproWeso,urrakenedgrrsnoouwpeexrapenosdpurl$ea3.ceindTthihenetroneo3wne-erxxeppoo1ss4ue5rddeeggrarotuohpu.pisn Measured orestimated exposure; estimated based onjob history information. dEexspcroisputiroen:lelveolws:expNoosutreme(ans=u2re8d9.), hiEgmhpelxopyoeseusrewner=e p75l2a)c,ednoinn-teoxp3oseexdpo(su-re8113c)a,tegories based on job gSuetsnaidtneigrs,ttihceaalnPdmCertaLchieof-edsspT:eacbiflSietcaAnSndaaMlrRydssiizseudsSiMynosgrtaesltmianstdoyafrtRdwaatlriioefsed(teavSbelMleoRpm)eedtahnbodyds9N.5IO%TShcHoe.nfeixTdpeiencscteepdrinontgeurrmvabamlescrwooefmredpedasetrhiasvgee1d.0 etuhsseteidcmoaamstreredesfbpeyornemdnuiclneigdpalctaay,uisneg-stpheeciafgiec, gmeonrdaelri,tyrarce,fearnednccealraetneds.ar pMeorriaoldittayburlaatteesdfpoerrswohni-tyeeaMrisnnofesfootlalnosw uwperteo Other methodological information: RESULTS -- - `nDgoernso-ucpmr.ailbiWeghnreaesnnutltthcsea:uesne1tsi4.r5e dSceoaMhtohrRst wwtehaarste awinedaerlneytziaefbdi,eodvSenM1thR(cwaconehcroeerrt:nooft6lt5heevoafetsteohdpehsfaeogrduemsa,otshltsiovewfr,etrhbeereiacsnattn,h ceurrhiitnygaphreyesxoaprnogdsaunfrsoe,r bhcalitagedhgdeoerrxi.peoassnuadrnedsjkwioenbr)(eewnxeocterpsttaalbtsriosetaliscetavlcalaytncesedirg)n.wihfieOcnnanltty,he2excocorehpo3trdtfeoawrtahbssadlwdiemeriretecrdaenptcooerrtaendyfoermeploayoefectehvheesre ecmapulsoeyspeedciifnic5 rWbeloamrdakdieenrresdcawwnhcheoernwttehharenedtaehtemapglweoneeyrreeadlanpiaonlpyuhzlieagdthiuoesnxipnoogfAsculorauebntjayombdasea(wteShrMerRatgs=r.e1a2tT.eh7rr7,eteh9am5na%l1eC21etm=ipml2e.os6y3emeo-sr3e7i.n3l5ti)hk.eelcyTohhtioosrdteifdefieeocdft ofpelfaabsnltta5dfodyreermaorcrsane(cetSrhM,anRan2=d0 ay2le4la.ro4s9f.,th3Ienodtbehsaethrpsrveeovdcicoduuersartcehodsh,ionr0t.e1mm2porletoxapyleeicettysedsw)t.hudoyA,hlal|dbolwfaodrtdkheeerdmcaihnnacdheirwgohdreekaxtephdowsaautrsehreoepboDsretcfeaodrt.uart T`(e9wx5po%osduCer1aet=ghrso0u.wp3e.7re~Thr1ee1p.o1Sr0Mt).eRd Fffioovrrewlcoiavreskrcesrocsafncwceihrr.orhwoeOsrnieseeowmfaptslhoeyinelvdtehrienwlceiortwheeerrxeippgoosrhtuoerrde ilgnorwtohuiepsxpcaoonhsdourrtoe,nje2obiisnnwttahhese hh3ii.gg0hh8 eexxppeocstuerde gmorrotuapl.i|ty ienxptehreileoncweeixpoasnuyroeftghreosuep,garonudps2.inthe non-exposed. The observed did hot exceed the "SrTethpueordreytiwsnetgrrteewnisgcmteahlsalsanmnuadmnbwyeeradskeanotehfsssd;eesha:othwseAvienrl,magatenhreyccooohfhootrrhttewicaastfaeifgroolrlyileyosowuefdnorginmaantlhdiessthueapndndautemesbpoeefrcoitahfleldyoeraiftgohirnsfaliesmssattliueldlsy,simntahaullsi categorie, thus limthieptoweironftghe study. Death certificates were locatedfor 96of%the cohort, but sas ENVAMRD(2002)17FINAL teshxuepbo-6scnuoorhteoorcbsaatwieganoserdiveecsroybualssdmeagdlrle.oantljyBoiibomldpoeagsiccctraitlphtemieroanesssuuwrseeromfeetnuhtseseadnoafalsfylasuesosurrosmciohngecametietchafelosrnuwemxepbroeesrunrooeft.daevaTathihelsacbiaent;meogtoshretireeosffoatrrehe.e meDxeopcroaestuursrep.eecmifpBilacosyieendestohnisintuhep19d9rae5ts.eulttohsfeotfgtheehomesettubrdiyio;cmohmnoeiwtaeonvreiron,fg PtchoFenOrdeSucitisnedstfiilollnmaepmlppaolntteonyteeimeaplslofioynreaemsrisawcnaldsaowmi0f.sc1aa3mt6ipolpneomoo.ff wHoorwkeevresr,weirnetehixspostsueddy,fofoithmeprlcahnemeimcpallsoyeinesthaerweocroknpsliacdee.red non-exposed. In addition 0 flurocheminle. Research sponsors: Universityof Minnesota `oCnoonnsliyste1 ndceyatoh.f rTehsruletesadIdnittohenaflirdseastthudsyh,atvheerneowwabseaennerxepcoerstseod.fbladder cancer; however, this was based CONCLUSIONS lWuoorrkoecrhsemeimcpallosyaerde aatt itnhcereDaesceadtruirskpolfdaenawthhofraormebleamdpdleoryceadncienr.jobs with high exposure to POSE-based REFERENCE MARleipenoxnraetn.sdoetr.D,ivBiHs.ionAporfilEn2v6i,r2o0n0m1.entaMloraanldityOcsctuupdaytoiofnwalorkHeearlsthe,mpSlchooyoel aotfthPeub3lMic DHeecaalttuhr, fUacniilvietyr.sitFyinoalf oTHERem erect set es te "This study isthe second updateofthe mortality study. 6 ENVIMRDQOO)I FINAL EPIDEMIOLOGIC DATA "Tiles. IdentificationofFuorochemicals in Human Sera. 1. American Red Cross Adult Blood Donors TEST SUBSTANCE Identity: PFOS and6other fuorochermicals Remarks: This is. final report. The preliminary report was dated June 25, 2001 METHOD . <1 Study desiga: Cross-sectional Manufacturing/Processing/Uses NA Hyrpeogtihoenssoifs theestUeSdS:, To determine the levels ofPFOS in the serumofAmerican Red Cross blood banks in Study period: 2000 Setting: NIA sTaomtpallespopfuiloamtiaodnu:lt 6donAoRrsC. Charlot. NC: Boston, MA; blTohoed 6barnekgsioinnsvainrcilouudsedg:eogrLaopshiAcngreelgeiso,nsCAin; Portland, OR, and Hagerstown, MD. thMeinUneSapporloivviSdee.d P6a4u5l,scMrNu:m `Subject selection criteria: Unknown Total#ofsubjects in study: 645 donors, ge 20-69 years `Comparison population: N/A Participation rate: N/A Subject description: The only demographic factors known were age, gender, and location. cHheeamlitchalesffweecrtes:stPudFiOeAd:, PLFevOeSlAsoAf, 7MSf7l0v,orMo5ch3e6m,icPaFlsOSinA,huPmFaHSn,blood serum, including PFO. The other Dyeaatraacgoellienctteirovnalme(2t0h-o2d9s,:30-E3a9c,hebtlc)ofoodrbaarckhwsaesx.requested to provide approximately 10 samples per 10- eaDhetonamiaaltsolgroy0anpprzhoydv/eaeitlddaeecatcrnooleslseptcritamiyoant:teoafndtheBmlomreoaldisabisslcpitreyact_ofrstoahmmeeptlarenysal(yHswPeeLsrCec/onEdaSunMcatSleyMdzS.e)d.Thues2ia4nngaslaytmhipiclgahelpslraewbseswruearsespblilltiiqanun1id0dd tthhee sitdeundtyittyo omfitnhiemsiezespelixtpesraimpmleenst.alTehreosreanalyses were performed concurrently with all other analyses of Exposure period: Unknown-PFOS serum levels usas suemogdate for exposure wr ENVIMRDQO)I FINAL 6wDee0rs6ec9ri120p5t2oirsormnoeoulprinwsehuabetjireocnttshorffeorewxbeoprtoehsuomrwaeelrgsroaunpdsf/eamtesgeosieins;cach33a2gemaelveedornyors(,30.33193, 0fe.m3a9l,e edoynocrsn. eTthe.e Measured or estimated exposures N/A Exposure levels: NA Statistical methods: A reliably sessment was done. Geometric means, 95% confidence nena of awdtdihesjertuersigibpeeurodtemisoeoenntrstr,ei1dcw.0e-rymeBaeoarconataslsg,tuerlatirpeandnea.grneva,allCyssei,innsttgeeawrnlqaduseatrred,tniodaleennndc(ryt0aenacgened,cedrriaaaitwbimuoeciuanomnnutloseafemtri.tuvmeeMPd9uFt0lOs%iSbapyaielrgbcoelernsetieorlenr,edcgaaocnehdanfn6rdaeoeqvpusueepnmscn.y A elabily assessment was iso done fohre chemical snl Other methodological information: NA RESULTS bDeestcwreiebnethreespullits:samTphleesr(ers=ult9s offortPhReOeSl(aaiwteyll3an6alPyFsiOsAiannddiPcRtISt)ha.t there was 4 strong colon ePsROS i the only chemical that met the reria fr lognormal distribution based on the Shapiro Wik PTThhFeeOSrgaenolegmveeetlwrsiatcshma<neLafneLmOasleQersu.(4m.3TPhFpepObSg)eeo1mveet1r6fi5co6ampleba.anctfMoiarolanelssl ahmnaaddleigsennwidafesira3w7natsy3p4hp.is9gh(pe9rp5b(%(pC9<TS.0C53H8),.5g3e34o.0m.3e3-t)3r6mi.c5dmppuwboo)e.n. 3N1u.o3rapchpemifocralalconfceemnatlreastio(n9s5.% C1, 300 34.3). Age was no an porn: predictor of sd sem TCWhhheeinoltohse,triNPfCFcOd(SSbLgyepogpmebeotg.rriarcpahnmigcee:alnos1ca9at:nod3n r1ta6hn6eg.e0hs)igbahynedsltohcgeaetliooownmeeswtterf:mreoamLnBoosfsotAronPgneFl0Oe5Ss.,0wp4ab0s,4nproathgnee:(s6a.m64p-3le82s70f53o0)m1 (2M72i0.6s10n),)ca3p0TldhieHSactgu.meurPlsaauttlo,iwvn3e3(.96190.p5%)b.we(Proe7rthl-iagn0hd7es.atn0df1oBrPoCosrtthoannrdw.ler2re7m0eapci1h05n3),l(Mo6wi.enr0n(a41p96o.3l40i)nsdSHa.gPer4as57.tro(wa1n,p7e3,5d.L3)ope,pAn0gl6es (aT2dh9ue.s0irpeesdpublft.soLf1o0os-myAenaagreablogeoet(sn3tt5ae.pr0aaplnb,a)l,ygseiMnsid,nernd,oenaaepnodtlotihcsa.ilcPtualruaaltce(t34mi.oapnnrpsmersuamnfdPeHFauOgdSersvinatlosuweinsl(3fo4o9rupexnyceh.6foHorosBwteionosnsrs oihbe.me for Charlde was much ower (39.0 poi) and he can for Portand wis alg Highs (5.5 ccBoonooftisntdreafnpceiainlndailiytetsoeonsff2fo0c070.e.P00FpOpppS.b.caAlTchuheleamtleeoaadwnemseotfatnotlhoeefra9tnh9ce%e9io5et%ralannecaelayizneidtl(iw3m0ait%s)o,f1(55he7.aopnblywowiirtthhPaRannOSwupeaperer 997055.%%7 ob wih anupper95% confidence init of74. pb. "The highest serum PEOS messurement nti sp was 165 ppfom male blood dor, 67 yeas old us ENVAMRD(2002)17FINAL PfmoarrlotemlsanPrdoe.rptrlTaenshdee.ntnienTgxhCte8hhnaiergxlhteoshttig(sheners=ut)mdHPoanFgoOerSrsevvtaeollwunews(a3(s-r2a)3n,2ge9Lo1pspbA3--ngf2er92lo6emspa(pnbm=)a1lw)eaerndedonmoMeria,nsnu6er2aepdyoeliainrss4'.ofledmPaaalullesso(anfnord)om saPinRgdnOiSfMic7aann0dt Pp(rrFedO=iAct2ow0r)e.sreofsBtPortFohnOgSlPyFincOoaSrrmAeulAlattiaevndadr(irMa=bSl7e.063m,)odPaedFlj.OusSthPeadFdOfoSarAlaAogweew,ragsceontrdhreeerls,artoiaonnngdewrtihteohifrPtiFhmOerSaAcitnAidoe(npr,e=nwdee4rn2te) avsasroicabilaetsi.on bAegteweeanndPFgOenSdaenrdwPeFrOeA.not significant predictors in models that examined the significant Spotpuudlyatisotnreonftgthhes UaSn,d weaknesses: Blood donors cannot be considered representative of the general Research sponsors: 3M Medical Department, Corporate Occupational Medicine `pCooonlseidstbelnocoyd soafmprleesusl.ts: Mean PFOS levels reported in his study are similar to those reported in other CONCLUSIONS ul NA REFERENCE 2Ou0lo0sr2e.on.cheGmWi,calBsurirnish,umJaMn,seLrua.ndb1.ergA,merJiK,canHaRnesdenC,roKsJs,adMualtndbello,odJdHo,norZso.belF,inLalR.reporItd.entFiefbircautairoyn 25o.f 349 ENVIMRD(2002)17FINAL EPIDEMIOLOGIC DATA "TTihloeu:ghtISdudes,nSteaitolef,FiWlaucsohraionctghteiomniocanls in Human Sera. IL. Elderly Participantsofthe Adult Changs in TEST SUBSTANCE Identity: PFOS and6ther luorochemicals Remarks: The resuls reported are final METHOD Study design: Cross-sectional. ManufacturingProcessingUses NIA seHloydupepor.ltyhepseirssotnsesttoepdrovTiodedeatmeormriensepetchiefisceurnudmercsotnacnednitnrgaotfitonhseodfissterliebcutteidonfolftorhoecsheemciocmaplosunindsa isnamtphlseagoef Study period: 9292000 Setting: NIA "stTuodtya.l population: 238 serum samples from elderly adult donors from the Adult Changes in Thought cSpohulablsajbeeocrtoaftsievtlehelicsytbiceoontmwcmeruientneirttihyae-sbUa.DsooenfdoWrpasrsowshepreiecnt6ig5vte-a9on6cndoyheoGarrrtosuospltHdu.edaylSutobhfjCeodcoetpmseewrneatrtieaivieadne(ndHtiMfnOioe)rd.mdaulErliiangggiibanleng iecnnodrniovdliuldcmuteaenldts were those with no known historyofneuropsychiatric disease or dementi. Total ofsubjects in study: 235 `Comparison population: N/A Participation rate: N/A SaudbjreecstiddeedscirnitphteioSena:ttl2e38aardeualftosr 531.83 ymeaalress,,ma1l2e0sf5e0m.a2leyse.arsT.he mean age was 76 years. Female subjects Health effects studied PFOS serum levels in blood, as wel as 6 other luorochemicals. eDlaetcatrcooslplreacytitoanndmeemthmoadsss:spBelcotordomseetrrays(aHmPplLeCs/EwSerMeSMcoSl)l.cted using high-pressure liquid chromatography! Exposure period: N/A 350 ENVAMRD(2002)17FINAL 8De5s4c1ri0p9t6ioyne/adresl(inne=at2i4)o,n of exposure groupscategories: 65-75 years (i= 121), 75+t 55 years (n= 03), Measured or estimated exposure: N/A Exposurelevels: N/A cSiatnlatcteuirlsaatctitecidao.ln tmeCemetsnh.tordaAsl:treelniAdarbeiintlchiytmyeataniscd smdeiesatnsrsi,sbwumtraiasoennagnleosstf,otgdheoeonemd.eattraicbymaegaen,sgeanndder9,5l%occaotinofnidaenndcethinitrerrveaslpsecwteirvee Other methodological information: N/A RESULTS I TpDbhe.secgreiTobhmeeertreersiwuclatsmse:naonsoigfnPifFicOaSntf(orpa<ll s05a)mpdliefsfewraesnce31i.n0gpepobme(t9r5ic% mCle,an2s8.8f:o3r3m.4a)l.esTahnedrfaenmgaelwesas I3n.4si-mp1l7e5 p"lTibhn.eeamr arengreosfstihoen9an5a%lytsoelse,raanpceewlaimsitnefgoratPivFeOlyS(wpas< 8045.1) apspsbocwiiatthedanwiutphpePrF9O5S%incomnefindebnuctenloitmiitnowfom1e04n.,0 eSPltFdeuOrdlSyy.lsetvVreeelnrsgyitnhislthaeenddSecswcaeraitkptnireevsgesioeinsn.f:orTmToahteidsoaented,aabttoahuetayrteahrceerostsuhsbe-jseoecnctltsiyiosndaaaltvaadialatavabalieul.sabelTdehtecohdasreuabtcjetercemtrisinzeoinnPlgyFsOcehSraurlmaecvtPeelRrsiOziSen levels in the elderly Research sponsors: 3M Medical Department, Corporate Occupational Medicine `Consistency of result:Todate, no other data have been collected on PFOS serum levels in elderly. CONCLUSIONS - PFOS levels in these Seattle residenatres similar to those found i adults and children in the U.S. E REFERENCE e Oestlnusideyfn..icSaeGtatWito,lneo,BfWuarslihusio,nrgoItcoMhn,.emLiuFainlndasbleirRnegph,ourmJt.Ka,n3sHVea1ran.Csoemn1,p. anKEJyl,d,erMMlaeynddipecalar,ltDiJeHpc,airptZomoaefbnnetttlh,,se ALRd.ult CFhebarnugaersy in25T,ho2u0g02h.t 3s ENVAMRD(2002)1TFINAL EPIDEMIOLOGIC DATA STtieeep:tocIocdcieClninitcalioTfrfiFaliluoIcnrvoaecshtteigmaiitciolonsinnHumanSera. 11. PediatricParticipants in a Group A TEST SUBSTANCE eee Identity: PFOS and6other fuorochermicals eee ee Remarks: The results reported sre final ee Studydesign: Cross-sectional. Manufacturing/Processing/Use: NA cHhyiplodtrhenestiosptrestoedva:imTodoreedestpeercimfiinceunthdeerssetranudmincgonocfetnhteradtiiosnstorfisbeoulfetcttiheeodsneflcuoomrpocohuenmdiscaisn cihniladrsena.mple of wSetruedyanpaelryizoedd:inCShpirlidngser1a99s9.amples were collected from January 1994 to March 1995. The sera samples Setting: N/A "Total population: Not reported iDSnuefbpejacertcitotmnessnetilneocchftiiloPdenrdeinca.rtiritceTsrh.iea:cThihTlehdyerenwseewrreaeresoabrmetpsaliiednesendtwseforrfeom2p3raosvtlaiatdreegsedinctlotihn3eicMaUlS.btyriTatlhheeosneUncGihrvioelrudsrpietnAyporsfetsreMenpittneondceoswcoicttahla signs and symptomsofacute-onset pharyngitis. Al ofthe children had positive throat culures atthe intial Total#ofsubjects in study: n=599 children, age 212 years Comparison population: N/A Participation rate: NIA Subject description: 299 male children, 300 female children from 23statesand the Districtof Columbia. Health effects studied: PFOS serum levels in bloaoswdel,las othe fluorochemicals, dcDheartgoarmaectoeoClsglrepactprihioyon/temoleettchhteorrodessqp:ureasBytlftooaonddasneeamrlaymssaiassmspslpeesctwreormeectorlyl(cHtePdLCu/siEnSgMhSiMgSh-)p.resSseurrae wlieqrueidfrozen at 20 Exposure period: NA ss ENVIMRD(002)I FINAL cDheislcdrriepnti2on-/d12elyienaerastiolodn.of exposure groups/eategories: Blood serasampleswere collected from Measured or estimated exposure: N/A Exposure levels: N/A cSitanaltteeirusaltcuitecidao.ln tmeCemetsnh.tordaAsl:tlieanAbdrieilntichtymyeataincsd smdeiesatnsrsi,sbwumtraiasoennganelosstf,otgdheoeonemd.eattraicbymaegaen,sgeanndder9,5l%occaotinofnidaenndcethinetierrveaslpsecwteirvee Other methodological information: N/A RESULTS eet LC LE 6DTeh7sectgroeiob5me1e5tr.re0iscuppsmb:e.an MoaflPeFOchSilfdorreanlhofatd hseigpnairftiiccainptalnyts(pw<a0s13)7.h5igphpebr(g9e5o%meCt1r,ic33.m3e:3a6n.5)P.FOTShelervaenlgse twhaasn `ffsoecrmoalmPeeFst:OriSc4m0w.ea1asnp8p{8be.an5ndpeddpb3t5o.wr2iitsphepfboa,nreruaepscppheecrtaig9vee5lgy%.rocuopInnffibrdooeomntcsaetgrealip2mia(tn2a8lo.yfs6e9sp7,p.b0t)hpetphbmr.oeuagWhhoafetgnhee9st9r(a45t2i%.f8iteodploebryyanwacgheee,lrietmhiiett a`wnraaaslpyhshieicgsah.lesaptgr,eesawennadtsattnihooetnnsoifsgtonairgftiecdPantFtoOlydSe(clrpe<evaesl0se5f)gorarasadsuocachlilaysttea1dt0ew3ib2ty.h8gPepRnpOdbSe.ratwAe1lr2tehysoeiuamrgsih.latrhIeandcasrtiomswpetlhereelsnitnoactteasr,prrheogovrwieesdvseeidor.n itis diff1i0icntuerlprtet thse data given the limited sample izefocach genderTocaton subgroup. dSUatStu.adayvehaisiltldrarebenln.egtVchhesarraayncldtietrwlieezadikennsgecsrseispretusim:vePTFihnOefSosremlaedtvaietolansaiarnbecohcuirtlodstrshe-nss,uecbtjieocnatlsdiastaaviulsaebdlet.o dTeotdeartmei,nehPeyFOaSrelteevelosnliyn Research sponsors: 3M Medial Department, Corporate Occupations! Medicine Consistencyofresus: To date, nootherdatahave been collected on PROS serum level in children. LNCoONrCLoUvSIOONNSs NA REFERENCE. OAlusoerno,cheGmWi,calBsurinisse,raJoMf, chiLludnrdebnerig,theJKU,nitHeadnsSetant,e.KJI,nteMrainmdeRelp,ortJ.H,JuZnoebe2l5,, 2L0R0.1 Identification of 3 ENVMRD(2002)17FINAL EPIDEMIOLOGIC DATA a(TinPtdFleOf:Ae)maAline Creremolapstsli-oosyneectetoipocanlraitnliiccaainlpaalcnythsseoimsifstothrfey,s2et0rh0yu0rmoiApdnetrhfwolerurmopornoaoenc,dtahnDeeemscauatltofulorongafytl,eora(onPcdFhOeuSmr)iincaaallnyds.misePderiercsfaulllutossrufororvceotimalnlmoaanalcteee program TEST SUBSTANCE ee J Identity: PFOS, PFOA Remarks: METHOD erent LU Study design: crosssectional mMaanPnuRufOfaSac,tcutruinpge/sPlruoocreososciannge/sUuslef:ony FfalcoilriitdesproidnuctDse.caTtuhre,seAfllaubcraomcahemaincdalsAcnatnwemrepb,olBiezlegiinumthewbhoidcyh thHhoyerpmomotenhdaeilcsailpsasrutaremvseettieledlr:asnocefToevoxlpaurmnoitvneiaedtreioenamsnpolfaogyAgenreetsgwaeitnrepelaaanntadilDoyenscitsaotosuferretuhmmeplPhoFeyOmeaSetsoalinnodg2y0P,0F0O,cAli.nilcaolvechaemmiesatssur,edanjdn Study period: March 1, 2000. Enddatewas not reported. Setting: Occupational. 3M plants located in Anterp, Belgium and Decatur, Alabama. a"Troetaawleproepuelliagtiibolne:fo3r4i0ncAlnutsiwoenrpietmhpelsouyreveeisllaanncde.S00Decaturemployees working in the chemical plant Subject selection criteria: Voluntaryparticipation in medical surveillance program in Y2000. e"mTpoltoa#yleoefss(u2b1j5ecmtasleinasntdud4y8:fe2m5a5leA).ntwerp employees (206 male and 49 female) and 263 Decatur Comparison population: N/A pDPleaacrnattitcuwirhpcoamtipwolenoryereaeetlesi:gwieb7lre5e%pearnotgfiacegipmeapdtleidon.yepre7osd3ua%ctottfihoenthAaecnttipvawirettiriepcs.ippaltOainnntlgyaAnn1dt2w%5e0ro%pf tomhfaeltpehaeretmeipmclippolayoteyieenegssAannaotdwte7hre5p%Doeffceamtatulhree employees were engaged in production activites compared to 63% ofthe Decatur female employees. dySaouiublnjygeeccrtontdsheuasmncrpiDipeotcniaotnou:fr amlMacaollheeole,AmnphtlawodyeerTeposw,eemrhpamldoeyalenoewsaelrhkaaBldMinlleso,wpehworsopPrhkFaeOtdaSsfeae,nwdGerGPTyFe,OarAAs,STlhe,vaedlAs,LhiTwgehaernredssewilgifng-ilrfyeiepceoarrnittdeledy evmapllsoaynedveshsi.gDheecrattoutarlfbeimlailreusbi.n and HDL values. Comparable resultswere observed for Antwerp female Health effects studied: To determine ifthere were differences in the folowing parameters based on as ENVAMRD(2002)17FINAL caPhmFeiOmnioSstItrraPinFesOsfeAr(aaslleekv,aellist:noteal phhaeonsmdpahtdaoittlacoscgety, bi(glhiaermummbaaitn.o,cigbtll,uotoadhmeymuloregaltorabnniistnfr,eorgaesRneB,,Ccsar,sepaatWritnRaitCnees.,,agmlpiulncaototeslreea,tnscfcheoarulanestse),,rolcs.lliannliiconawle dsratetinimsoui,ltaayntdinligfpeoheporrtohmtyeorinonexsi,,nes)he,irguhm dtehnysriotxyinel,ipfoepreottehiynrs,oxianne,d sterriuglmycterriiidoedso),thyarnodn.inet,hyrtohiydr.oidhohrmoornmeosn.e (bitnhdirnog cmDheaeatmsauirsceiomylelneactntsdioohnefmmhaeettiohglhotod,gsy:wetiesgMthset,d,itchaaynrldoiqbdulehosootdriomnponrnaeeisrseum,reea,wsouurrrekinmaelhnyitss,itsoray(nDdequcpeaustltuimroonnonanalriyry)e,,foabnncldtoioosdntatsneedsrtasar.dsacVmlaipnlliuecesasl, used for reference ranges were not provide. ppDarerottvaiiiclidpseadniotsn. hdiTasthraeepodcroatlt.laeDcwateitroaneo: snelbfTl-hroeeopdorsctioetdel.-lscpetQcouinfeis(ctaimwoonounrnakti,rehticcs.to)onrtnyoetnq,puredosevtsiidioegndnn.,aiarUdermiinnwaialssytsriaastdimwoinan,siesottnce.lryewsdesreoessnaeoldtl RfoBrCsD.ecatur employees via standard urine microstick analysis which testedforurine glucose, albumin, and TdSeH,tefrerebmTy4icannldoenedTd3enwezryemeddetoenromriinmemdunboyasismamy.unoFcThIemwialsucminaomletcribucylmausalsatyit.pleyiTdn4g Ta4ndanTdHTBHRER.were (Stpheeerrafisuaeomxrptolroaeccsttsawneerseuwleefxrotenraamctieddaolauscoseitnagt)aanumsliioynngx-dphaiigrhi-npfgroeresxsturraePctEliHioqSnu.ipdroccherPdouFrmOea.StoAgAIrn,apadhdyitPeilFoeOncSttrAoo,sPprFaOyaAtnadannddemPMFmSOaSSsG,s sPpFeOctSroamnedtrPyFaOnAd weevraleuaabteodvevetrhesuLsLaOnQe.xtracted curve from 3 human serum matrix. All sera values for Exposure period: Unknown. PFOS/PFOA serum levels indicate exposure. wgDeeenlsdlcerrai.5ptiboyn/sdeerluinmeaPtFioOnSofdisetxrpiobustuirone,gprrooudpuscitciaotnegostraiteuss:(pWroodrukcetrisonwevsr.e nsotna-tpfrioedducbtyiopnlawntorkleorcsat)i,onansds MA=net2aw1ne5r)spe,wreutrhmee Pm0Re.Oa9Sn aPlneFvdOelSs1.fl4oe0rvepallplomfe,mmaprllesoepyeepcretoisvdepulacyrt.tiiocniLpeeamvtpeilnlgsoyiaen metsohinwsgasstpur1do.yd16autcptApinomtnwaeenmrdppl1(.o16y3e=ep2s0p6w)eatraenDdehciDagethceuarr.turA(t1 MLeevaenls PaFmOonAg lpervoedlsuctfioonalelmpelmoplyoeyeesewserweerheigh1e0r.3 `employes was 1.28 ppm and 2.34 pm at Decatur aAntdAn1t.w9e0rpp,pmtheatmeAantnwPerFpOAanldevDeelcoaftumr,lerepspreocdtuicvteliyo.n Measured or estimated exposures Serum PFOS and PFOA levelswere u(s0esetimdate exposure. oeSfatavctahirsithaiencmcaalet,moaleontgdhiocmdausll:tiavnDadersicacrblilipneitrciaevlgerescsihsmeipmolineswraeynrdetseutstrsaeaitnfdodidetvahanylaruloayitsdeesa,hsosProecmaiorasntoienonacssosrbareye.tlwaetieFonnorPcRoseOifrSiactiafenindetdsP,FamaOanlAaylsyacsnsid,s ecplomanpntsl.iodyaeenredesdywipneertheoefjdoaibnva.ildyesdesinitnoclquduead:rtasgeo,fBhMeIi,r aslecrouhomlPcFonOsSumdpitstiroinb,uticoing.aretPtoetuesnet,iayleacrosnfwoournkdeidngatfesietthoerrs Multivariable regression models were fited with PFOSIPFOA analyzed as continuous variables. Natural ass ENVIM/RD(2002)1 FINAL log and ttroaennshfaornmcaetimoondselofitt. heSdAeSpewnadsenutsevdartioaablneaslywzeertehepdearaf.ormed, when necessary, to normalize variables Other methodological information: a ADenstcwreirbpe Therefore, raensdultDse:catur employees univariate analyses were wienirteialdliyffsetrraetnitfieidn bsyevleorcaaltiwonayasnd(stehcen "tShuobsjeecatnadleysscersipwteiroen"starbaotviefi)e.d by sender eTmhpelroeyfoerees,. tahnedPpOrSoduvcatliuoens status. in the They quartiles waerreedifpflearceendt fionrtomaqlueartpirloedsucdteiopne,ndnionng-proondupcrtoidounc,tainond status. female AThne emeran PFOS level stratified by production for all status, employees at the mean was t1h.i1s6pplapnmt wfoarspr0o.d9u6ctpipomn e(mrpanlgoeye0e.s04an-d60..2442 ppppmm).forWnhoenn w`parsod0u.c1t3iopnpemm.ployees. The mean for female employees 0M:a2l9epppromdurcatnigoen, e0m.p0l4o-ye0e.s41weprpem,plaQc2ed(mientaon,th0e.5f8olplpowmi;ngraqnugaerti0l.e4s1 range 0.79.-- 1.66ppm).Q4 (mean, 2.61 ppm; range,1.67 6.24 ppm). b~as0e.d78onppPm)F,OSQ3le(vemles:an QI (mean, 1.18 ppm: h1Iin.g7mh6aesl--te6qp.ura2or4dtiuplcpetmiw)oenraeenmdspilgtnhoieyfeilecosawnet(snlty=(wpa15<s0)0,0.52t)h9eoplhdpiegmrhea(snrtdaqnwugaoerrt0ki.le0ed4mme-o0ar.ne4s1yeearprupsmm)a.tPFAOnPtSrwoelderuvpcetltiwhoaannsee2mm.pp6l1looypyepeeemss(iirnnatnthhgeee. pclrooowmdepusactrteiquodanrtbieylmep.qluoaTyrehtieelseownolefyresdieofrbfsueemrrevnPeceFdOifSnorcldtiihnsyitrcroaiilbducth(ieoTnmS,iHs,tnroTie4s,sigffnorirefepicrTao4nd,tucT(t3pi,onT<0Hw5Bo)Rrk,edirFfsTfIew)raeosnrceifnsorBaUhmNeo.mnagtoWlmhoaeglnye (HCT. HGB, RBC, WBC, platelets). The same held true for non-production employees (n = 56). For all quartile. females. feTmhaylreoiedmpalnodyeheesm,atBoUloNgywraesssuisgnwifeirceanntolty sdiigfnfiefriecnatnt(lhyigdhiefrf)erebnettwbeeetnwee1"n qaunayrotifltehaendqu3artialnesd 4 for DTehceatmueran PFOS level stratified by production for all status, etmhpelmoeyaeneswaatsth1i.s63plpanptmwfaosr 1.40 ppm production (range 0.11 employes - 10.06 and 0.73 ppm). ppm When for no. pprroodduuccttiioonn jeombpsl(ony=es1.61T)haenmde6a3n9f%oorfffeemmaallee eemmppllooyyeeeess wwaosr0k.e9d3inpppmr.od7uc5t%ioonfjmobas.leemplowyorekeedisn r0Maa5nl5geepp1pr.o3md2-ur2cat.nig2oen9, ep0m.p11lpo~yQme40e.(s7)m5weepa,rpnme,)p,3l.aQ2c22edp(pmimne;taona,tnhg1ee.0,f1o2lp.l3po1wmi;~ngr1a0qn.ug0ae6rtp0ipl.me7s)6.b~as1e.d30onppPm)F,OSQ3le(vemles:an Q1I.74(mpepamn;, tWhheeqnuamrtailleespwraodsiucntiAoLnTwo(rhkiegrhseswteqruearptlilaeceddififnetroenqtufarrtoimleasl,lt3heoothnelrys)s.ignifica(npt < .05) difference between FNToI).sihgneimfaitcoanltogdyif(fHeCreTn,ceHsGbBe,twReBeCn,quWaBrtCil,esplwateerleetso)b,soerruvreidnafloyrsitshy(raolibdumi(nT,SHb,loTod4,, sufgraere).T4, T3, THBR, 356 ENVIMRD(002)I FINAL `dFcioofrufnetrfeewnmhcaeelrseeatmehmoepntlghoiyrqdeuaeqrsutairl(tebisoletfhowrapdsreomsdiouggncritafipiohcniacntsal,nydltohnywroeorri-dpt,rhaohndeumtcahttei1ool"nogqyeua,mrpotuliorleyi,enebasu)tl,ythstesh4eerxecweawpyetrnefootr.nmoeasnignpilfaitcealnett lpWirhnoeidcunacltriecoshunelmtwsioswrtekrreyertsaensitanslyatznhededlhbiivygehrneestnuzPyFmmoeOfSacnbqmdupableriotlyiilerreuebsifnowtrehAsotL,hTta,hdevGraeGlTwu,esaasanhdigbtshotteahroleprleeirvfvceeerrneeptnaacngeeelroafntmghaeanlfeotrhDeheecopaattthuiecrr pq(eQur1ac)relnastn.adges2M8ofs%ort ionntoattlahbellievheiwrgehrpeeasnttehlex(prwoehssiuulcrthes ifgnorcrolAuudpLeTs(Qaw4lh)kealrhieande8p%vhaolosufpehseamtapablsooevy,eeAestShTei,nrtAehfLeeTrl,eonwcGeeOstTr.aenxgapeno,dsuwtrhoeitlagleraohnuedp edHmoipwrletovyeberiel,isreua(bcaihnl)ishuwobeu-rgpehop1tuhl8ea%tniaonnwdahs3a5do%an,ldyrifef5se4pr)ce,tnitivnsleyDr.eucmaTtPhuiOrsSftreqemunaadlretwsi,alseodrnisoitnreibvauintdiyeonno.tfinTthhDeeerceAafntoturwreenrtophne-yspmrcpoaldnouncyotetiebosen directly compared, Analyses combining employees from both plants ta`rnWidhgelynncoecnrl-iidpnerisco,adluaclctkhaielominin)setpr(nhyors=epshua4lt2tas1s)oef,aantloldtamlpallbaieclieerdmubpiilnno,tyoaeneqdusarAftrLiloTemswbeo(tnrhe=psliag1nn0ti5sfiwcpaeenrrtelyqcu(oaprmtb<ilien).e,05d)m(hebiaogtnhherpvraiolnduuetchsetif4oonr adaiunsagtrretilb0cu.t0(e4dme0aa.mno4nP2gFpOtphSme).lqeuvae1rtltisl2he.os6u9lw,dhiablneegntehoits1e.dw6a9ths-atn1to0h.eth0n6upcmabpseemrofotfrhaADnnectinawteturhrpeepfmripsoltdouy(cemteeisoa.nn eTPmhFpelOoShyieglheoesvsetern0.ue2m7ebveperonmlo,yf lDiegchattluyryeomunpgleory,eehsawdaasloiwnerthBeMI4, aqnuadrtwiloer,kedInfeawdedirtyieoanr,stovhearnalelmpthleoyeemepsloinyetehse oitnhtrhequ1arqtusartic were "saliTkghanylirifoniiecdapnrthelosysulplthsoawtfeaorrset(hpains<ds0Ga5mG)eTignwreQoru4ep stiihngadnniicfaQitcIea.dnttlhIyanthifTge3hmearwlea(spe<mspi0gl5noi)yfeinceasdntcltyootmahbliigbnhieelridr(ufbpoirn<bsog0tn5hi)fpaclnaandnttslTyH(noBwR=er9w7a)is.n aAQlnl4otwftehtarhnpemfinewmQoaIlr.eksedwMooartskttehdeofDientchnaeotnuD-repcrpaloatdnuut,crtifoenmajloebs.empTlhoeyreefeosrew,orQk4edwasin9p2r%odfuecmtailonejporbosduwchtiiloen wmoorskterosfatnhde aTmlehkaeasluicrnoeemsb,pihhnoeespdlheapvtleaalsnset,indcAarSaeTas,weedrAefLrTao,nma1lGyGzTe1,d0 Qfa4on.rdeImtnoptQlaIlo,yle4iev%serowfphatnoheel.heamdplFvooaryleuemesaslahebaodevmvepalltuoheyeseerssebfoefvroeerntchaellrroaefnfegrteehnefcsoeer upaamnnbgelee,rfs1o4ro9fA%foeLfmTathlaenedeem6mpp%llooyfyoeereesGsGhwTaid,thvwavhlaiulleuesesa1ba2ob%voevwetahtsehrreerpfeoefrreterenedcnecferorarnaQgn4egeifnwoarQsIbovtaehsrycoofsmmtpahaelrse(endte~tstos5,)3.3%ForintOot4a,l iTvheer ahwleseroeaacboopnovdseuicttdieavdte.aswigTenhrieefriecnaonwttaas(dpjau=sptoes.di0t5i)fvoaersspsioogtcneiinafttiiicaoalnntcb(oepntfw=oeuen.nd04e)rPsaF;sOstoAhceiraaetnfidoornec,hboemlteuwslteieevrnoalrP.iFsObWSehearnengdrbecoshtsohiloePnsFtsrOnoaSllyaasnnedds waPsiFstohOcAiHatDweeLdrewailitfnhcoluPugFdheOdSPiF(npOthA=e.0mw1oa)dsealnn,edgnaPetiiFtvhOeelAry w(aepsrs=eocsi0ia0a2tt)es.dicwaWilthlhey nHsiDbgonLitfh(icpwaen=rt e.a04e)p.f=.i0Tn5r.tihgelPyFmcoOedrSeildwe,asPswFenOroeAspisogesnimitfaiiivceanlneyt `ssGiiggGnnTii.ffiiAccaaSnnTtt (aopsrs=otcoti0aa2l)t.iboilnTisortuwbailenr.oergAoabnssieicgrnfvilefudiocraiwnniteth((pTP=OFFO).S02w,)apPsoFshiOitAgih,vleyoarssioTgcnOiiafFticiaionnntrwfeaolrsattioioblnsyetcroevreaidldkefasolri(nTpeO=pFh0o0as0pn9hd)a.iAaLsTNe..o Asigpnoifsiicavnet s(ipgni<=fic0a0n0t1()p i=n0t4h)e amsoodceil.atioBnMbIe,twceiegnarTet3esainddayP,ROalScowhaosliodbasyerwveedr.e aPllsaontsilgonciaftiicoanntw.as(hIinghtlhye as ENVAMRD(2002)17FINAL aHulnotiwvheaovrueigarht,ethfaeonradlicyfasfceehsr,epnlcAeansntww(eeirnredpivneiomdtupaslltoalytyi)esetTisc3ahlvlayadlsuihegisngihifenirccraenmates]aendTHbTyE3Rqu,laervatesillsweeltalhsasnPFtDOheSecaotstuehrreruemtmhplylervooeyiledsehsinocormveemarsaeeldl.,. wawneedrreeFTpnoIos.titwsiievgrneeilfyinacosatsnstoicgiinnaitfteihdeca(rnetpgl=ryaess0ss4i,ooc0ni1aa,ntaealdnysdwcis0.t0h4M,PoFrseOtsSop,efctPthiFveOeAlty,h)oywrriotiThdOFTh.3ormoHnoewse,veirn,clPudFiOnSg,TSPHF,OAT4o,nTdHTBORF. wAStneutldwlyesrpisntprPoepFnugOltSahtssioeanrsnudmwlewerevealkssin,genPsisFfeiOcsSa:nstleyrCrudomisfsle-ersveeenclttsiaionrneacldeercdaleiisningidnne,gmtovhgoerlrauepnbthyairmcyaakpniadnrgtcilctiipmhaeatarildoencr,hoethmdeietsDeteccteaatsnuurelfsfaon1edt. iosfnuolcyhneoasniesPpmreeFasseuanOr,reemppleAranentsta,epnoatpuicnletrthtiaoinnpslpaconasnn,tnoitn tbiemecowmapsarceoldlebcetceadufsoerqcaucahrttess,aortehdeirffpeerrefnltfuoorrienatcedhcshuebrgmrioeuapl.y Research sponsors: 3M is`neCcothniesominsaattleonlmcoeygdiicocafallreassunurdlvtesci:llilnaiTnchacelesdceahteraem,siuislnttrsthyaatrpeaalrlsaoomfmettehewerhsmatocfocotnnhcseliusAdtneetntwtheatwriptthhorrtehwDoeescreaeotnfuorthwseoirgkn1ei9fr9is5c.aantnNdaobn1o9dr9em7callicinrteoiseisnsddciahftofaleersdetinedtronflortloemrveehvlaesatwlacaosnlloyebcsstieegrdnvieifndic2aan0st0P0cFhaOanSndgseteshreruienmfohlreeepvaectlasincinnoocrtrelbaisepeidcd.omclpTianhrieecadhl.ocrhmAeomnileosntdgraiyttuavdacilonulaellse;catnheadolywnseivse1ro9,f95tthhweeasrsee were many limitations o the study. CONCLUSIONS Jp"iTrphooedpuracoutttiehioonnrs,saocnrodhnecnplaoutndi-ecpdreontdhzuaycttmietoshnetsheeatmdpaalrteaoycceoonensstisitndeou.net n(wo0ittshuhtgaogvxeeisctosltiohgganiticfaiAlcnaftnitwnedircnhpgasnaignnedslaDbieoncraattsuoerrryuMamunoircmohcaohl.eersmtiecraoll REFERENCE oOpelmrsofelnnueoGr,Woo.chteBamnuaertslouellwfoogMnyaMta,en(dBPuuFrrrOiiSns)alJyaMsn,idsMpraeenrsdfulletulsorJfoHor.cotmaOnmocaalteet(ao1PnF1dbO,fA2e0e)0mr1ai.lnereeAlamctpriolosontsy-oesceelciptnaiiroctnaiacllipcaahnneatmlsiyssotifrsyot,fhetshey2rr0ou0im0d DAeaptawretrmepnta.nd Decatur flvorochemical medical surveillance. program. Final report. 3M Medical 355 ENVAMRD(2002)1 FINAL EPIDEMIOLOGIC DATA T(PiaPtrFlteOiscAi)paAnLtesvLeoofltnsghieitnu1dR9ie9nl4aa/lt9i5oA,nna1tl9oy9s7L,iispaindodfa2n0Sd0e0rHueFmplautoPirecorcfClhlueiomnriioccoaaclltaCMnheeedsmiuiclsaftlornySautreTvees(itlPlRFaenOsc)eulPsarnofdgrroPamemrMfalluoeroEomcptalnooynetse T TEST SUBSTANCE ee Identity: PFOS, PFOA Remarks: TO ee ll Study design: longitudinal Manufacturing/Processing/Use: 3M Decatur, Alabama plant and Antwerp, Belgium plant. tHoypcohtanhgeessisintecsltiendi:calTcohedmeitsetrrmyinaenwdhleitpihdereocscuspatinioenmaplloeyxepeossoufre2o3Mflfoarcoiclhiteemsicals over time is related S1t99u4d/y95p,er1i9o9d7:, aTnhder2e00w0e.re 3 time periods during which medial surveillance took plaatctehe plant--s Setting: 3M plants in Decatur, Alabama and Antwerp, Belgium "Total population: periods. 175 male cmployces participated in 2000 anda esst one oftheothersampling `Subject selection criteria: Employees participated voluntarily. Comparison population: n/a Pp1aa9rr9tt4ii/cc9iip5paatateniddoin2n0r20a0t00e0:.(n 1=20466/%51,p7Aa5rnt(ti6wc1ei%pra)ptep4da5r,itiDcaeilcpaa3tteusdrami2np0l)1,i9na9g4n/dp9e53r,i9o%d1s1(0(a/n1=7=5649(1,,63AA%nn)ttwwpeearrrtppic32i40p,,atDDeeedcciaanttuu1rr9923709,)),ai3nnd71%1979p57n and 2000. `Sgceunobenjrsaeulc,mtptdAieonsnctrwoiefprtapilocnom:haollMearleeepmoperlmtopeydleoteyhseaenswDeverceaotsulirgnumiafnilcettaonetpemlapyreltoiyrycoeiueepnsagd.teer,Ain nhbaitdomualnoiwmteaorlreiBneMgmIposflfoeyareneeddsataaltshhoeighphlaeadnrtsld.oawielTryn mDeecaantuarlkmaallieneempphloosypeheast.ase and triglyceride values and higher total bilirubin and HDL values than the HafefaelctthedebfyfePctFsOsStuadnidedP:FOTAo ldeevteelsr.mine whether workers! lipid and hepatic clinical chemistry results are dtDreaantnsasifteycroallsilepeoc(ptGrioGotnTe,imnesIUt(/hLHo)Dd,sLa:,spmagrC/tladitnlie)c.aalmtriincgohlteyrmcaiensrstirfdieesersa(smeagn(/dAdS)hT,e,maalItkUoa/llLio)n,geyaplchaonolisnlpeehcaattmaeisdne--oct(hrIoaUln/esLsi)te,rraogslea((mmAgLT/l,du,ItUah/iLmg)yh. total and direct bilirubin (mg/d). Demographic data collected via questionnaire. 350 ENVAMRD(002)17FINAL aDdemtianiilsstroantidona,taccc.olalnedctbiolno:od cDoeltlaeicltsioonnmdaaecoltwleecrtheinonootmpertodhvoiddssedi.ncluding questionnaire conten, design, wePitFtrOhaSbuettahynydllamaPmcFeotOnaiAtuemmaetnotdhioodtnhs-epaoiaefbswatinrtaahlcytsPiioFsnOSdpirfaofndedurcetPd FswOlaiAsghtilntyhtehnceacsahenraulymey.azre.dThueIsniinogn1-9p9hai4ig9rhs5-,pweertrfheeortmhmaeenntcehe.ondtrhaqucusiieeddd sccehhrrraoommsaaattmoopgrlrgearspahp-hwtyeh/remeramseosxstsprpraeaccyttermdoamseustsriyns,pgeucastnironmgieotsnre-ylp.eacitreiIdnngf1on9ex9tm7ro,ancittthieoornsinepgrruoimcnedstuahrmeep.nleegsatHwiievgr.hei-opanenramflooydrzeme.adncbeIyn 2li0q0u0i,d liquid ecxhtrroamcatteodgrcauprhvye/erloemctarohsupmraayntsaenrduemmmmaatsixs,spectrometry was used. The samples were evaluated versus an Exposureperiod: Unknown. PFOS, PFOA levels measured in blood serum. ssDuuerbsvpceoripiluplltaainctoein,o/ndBselciAon,netaBat,iinoaennddotfhCo.esxepeAomspuwlraoesyegceroosmuppwsrh/ioesaeptdaergtooifrciietphsae:teedmTpihnleo1y9eg9re4os/u9pw5shoaonfdepam2rp0t0li0co,iypeaatenedsd Cwiencroaenltba3rionkyeeedanrtshionsotefo Who participated in 1997 and 2000. a`mMbeiaesnutreedxpoorsuersetidmaattaeadreexavpaoisluabrlee:. PFOS and PFOA levels were measured in workers' blood serum. No. Exposure levels: AntwerpMean PROS levDelescatur 1199997495 114827 ppppmm 216825 ppppmm 2000 116 ppm 167 ppm 1199997495 2000 AntwerpMean PFOA levDeelcsatur 1154 0pSppmp.m 1.90 4p1pppmm 1.43 ppm 183 ppm ruStesagitrniegsstsSiiAcoSanl.mmoedRteehslotsrdwisec:treedrbeumpiaelaxttiebmdyumimenatlsriuokdreuelcsiihnoiogndcaolerlspctoiormavatatirenisgaoetfshveaanrrdaianendscoteimnpgsutbahjeecrctoveaafrfwmicaetnercfeeitcesotdmreupc(ut0rturaeesd.m.ixCoeAvddajrmuiosadtteeelds included inthe model were age, BMI, numberofalcoholic drinks er day, and cigarettes smoked per day spOautrrhtveiecirilplmaaetntechdeoidenoxltahomegi2sc0ia0nl0ceisnu1fr9ov9re4mi/la9l5ta.inocne:TyheeAarretafoltoasrlo,opfatrh1tisi7c5ipprmaoatvleieddieemdpalatonlyeeoaspetpso(or1tnu0n0pirteAyvnittoowuesurnfpdueaornrtodackh7e5emaDieclcaoanltguimrte)uddiwicnhaaoll assessment, 360 ENVAMRD(2002)17FINAL RESULTS WPhFeOnSmreesaunltsserum PFOS levels were compared by sursillance year, PFOS levels have been decreasing in (thheospeartwihciopanptasrtiincimpeatdeidcailns2urvoerilmloarncee minedbioctahlpleaxntasm.s Wbehtewneetnhe1d9a9a5 waenrde2a0n0a0l)y,zeAdntbwyetrhpe a3nsdubDceochaotrutrs eenmtpryl.oyees in cach of the 3 subeohorts had lower mean serum PFOS levels in 2000 than at thei year of eWmhpelonyeaensa,lytzheedreuswiansg nmoixaesdsocmioadtieoln mbuelttwievearniaPblFeOSregarnedssisoenruamndchocloemsbtirnoilngorAntrtiwgelyrcperiadneds iDn'ecmataulr pianrHtiDciLpanatlskaolvienre pthiomes.phaTthaesree, GweGrTe,aAlsSoT,noAsLiTgn,iftioctaalntbialsisroucbiina,tiaonnds dbiertecwteebniliPruFbiOnS, and changes over time WPhFeOnA mreesaunltsserum PFOA levels were compared by surveillance year, PFOA levels in the employees dp1ea9rc9tt4ie/ca9isp5eadtainsndlgigi1hn9t9lm7yedbaienctdawltehesenunrvi1en9ic9lr7leaaansnceedd a2bt0e0tt0hw.eeeAnAnLt1wt9eh9er7pDaepncldaant2tu00ri0np.clraneWtah,seePdnFbOtehAtewdesaeetnrauw1me9rl9e4ev1ae9nl5saladynezdcerde1ba9sy9e7pdlabanendttwtaehneednn mnthoeeacn3onsPsuiFbscOtoeAbntolrcethvsealn(sghteoessneadcewrdhostosopisanurcbrteicacosihep.oatretdaint2heoArnmtowreerpmepdliancta.l eHxoawmesvebre,twaemeonng19t9h5e a3nDdec20a0t0u)r,stuhbrceohwoerrte, eWmhpelnoyeaensa,lytzheerde uwsaisnga smtiatxiesdticamloldyeslignmiuflitcianvtarpioasblteivereagsrseoscsiiaotnionanbdetcwoemebninPiFnOgAAanntdwesreprumancdholDeesctaetruolr a(sps=oc0i0at0i8o)nsanwderteiglliymcietreiddesto(pth=e00A0n2w)eorvperetmipmleo.yeWeshe(npa=na005l)ybayznpd,leadinn apanrdtiaclusloarb,ytshuebc2o1horAtn,owtheerspe ePmFpOlAoyeaensd wthrioglpyacretriidceispatweadsinalasllo 3sustuirsvieciallllayncseigyneiafricsan(tp (=p =001)0.2) Hfoorwesvuebrg,rotuhep aBsso(ceimaptiloonyebeestwweheon PpaFrtOiAcipaantdedtriinglbycieormiodneistaomrionnggiDnec1a9t9u4r/9w5orkaenrds.2000). There was not a significant association between p`Thhoesrpehatwaesree, GnGoT,sigAnSiTfi,caAnLtT,astsootcailabtiiiornasbibne,tawnedendiPrtFObiAliruabnidn, changes over time in HDL, alkaline `WTeohmtpeallnoyOeraegnsaa,nliytczheeFrdeuouwrsaiinnasge s(mtTiatOxiFes)tdicraemlslouydltsesilgnimfuilcianvtarpioasbiltievereagsrsoecsisaitoinonanbdetwceoemnbiTnOiFnganAdntsweerrupm cahonldestDeercoaltu(pr s=ign0i0f7ic)anatn.d Tthriigslayscseroicdieasti(opn w=as00m8o)reovceornstiismte.entfHoorweAvnetrw,ertpheminptleoryaceteisonthtaenrmDewciatthurtiemmepl(oyyeeaerss.) was nor Stud1y stArveenrgtyhssmaanlldnwuemabkenersosfeesm:plSotyuedeyslipmairttaitciiopnasteidncilnuadle t3shetufodlylopweirnigo:ds (only 24%,1= 41) 32.. cdiofufledrennottlaanbaslwyezreefuesmealdeeaecmhplyoeyaerefsodruaenaltyossimsaallndnduimfbeerresnt analytical techniques for PFOS. 4. lPaFboOrSatloervyelasniimnaelmsployees are decreasing over ime and are blow those levels causing effects in 65.. mseorruemAPntFwOeSrpleevemlpslwoeyreeesaptphraonDxeimcaatteulrypa0r.t5ipcippmatlinogweirntihniAsnsttwuedryp(5em7p%lov3ye4e3s%t)han Decatur 87.. tPhFerOeAarleevseelvserfallucctounastiisntgendtirdeicftfieornendceepsebnedtiwnegeonnthteheApnltawnte)rp and Decatur male population (eg. st ENVAMRD(2002)17FINAL cshateimsiiscatlylyprsoifginliefsi)cant differences in BMI, age, consumptionofalcohol, and differences in 9. cloomwplaerveeldsowfitPhFtOhoSseanthdatPcFaOusAemeefafescutrseidnilnacboarcahtoprryoagnriammalysear among these employeeass 10. blood sampling was conductedonlyoncepersampling period at a certain point n ime. Researchsponsors: 3M `thCeonlsaiststseinnccyeoPfFrOesSulitssb:eiTnhgisphiasstehdeofurt.st longitudinal analyosfithse surveillance data and wil probably be "yTphoelipposiidteirvmeiaasesfofceicattioobnsebrevtewdeeinn ProFdOenAtsan(dansdenroutmocbhsoelresvteedroiln apnridmattreisg)l.ycerIindeasddiistinoont,ctohnissisetfefnetctwhiatsh ntohte batetehneoDbesceartvuerdoart A3nMt'wserCpotpalagnte. Grove facility where PFOA serum levels in workers are much higher than CONCLUSIONS _ : SAiglnoinfgiictaundtincahlanagneasl,ysicsonosviesrtaentsixw-iytehartpoexriciooldoogfic1al75daAtnat,weorfpliapnidd Doercahteupratmiaclecleinmipclaloyecheesmidsidtrnyotvaslhuoews aGhsosloecsitaetreodlwaintdh PwiFgOlySc.eriAdesp.ositWihveensttainsatlyczaeldy bsiygnpilfainctantanadssaolcsioatbioynswuabcsohoobrste,rvtehdesbeeatswseoecniatPiFonOsAwearnde sluirmvietieldltaoncteheyeAarnst.werp employees and, in particular, the 21 Antwerp employees who participated in all 3 REFERENCE OPlesrefnl,uorGoWoc.t,anesBlufrolneawt,e (MPMFO,S)BaunrdrisP,erfJluMo,roocMiaanndoaetle,(PLHF.OA) LAeveLlonignitRuedliantailon AtnoaLliypsiidsanodfHeSpeatriucm: CFlliunoircoalcheCmhiecmailstMreydicTaesltSuRrevseuilltlsancferoPmrogMraalme. E3mMplFoiynealeRPeaproircti.paOncttsoboefr t11h,e2010919.4/95, 1997, and 2000 0