Document gE8R4mBDGXb60pV8VqEapzrL3
MICROBICS MICROTOXID TOXICITY TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFX-3106. (IOctanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 015G. The testsample is FX-3106, PO# S5233-004, EAI 85548, Sumitomo 3M Japan. Current informationindicatesitisa mixtureof 2.31% PFOS, 2.6% diethyleneglycol butylether,83.38% water,10% ethyleneglycol,1.36% Sultonefoamer, 0.2% xanthan gum, 0.1% 2-biphenylols,odium saltt,etrahydratea,nd 0.05% benzotdazole.
The followingsummary applies toa mixture with incompletely characterized concentrations of impurities.Dativmay not accurately retlectthe toxicityof the tluoruchemical component of the testsample.
METHOD:
Method: MicrobicsMicrotoxD "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1985 Species: Photobacteiiumphosphoreum Analyticalmonitoring: pH and osmoticconditon. Exposure period: 15-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program, e.g.,probit. Test organism source: Microbics'Reagent. Test conditions:
Diluent: MilliporMeilli-QT'Awater Test temperature: Not noted. Stock and testsolutionpreparation: Not given. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESULTS Nominal concentrations: Blank control,10,100, 500,and 1000 mg/L
Element value:
5-minute EC5o = >1000 mg/L 15-minute EC5o = >1000 mg/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 15-minute EC5o forPhotobacteriumphosphoreum was determined tobe >1000 mg/L.
Submitter: 3M Company, EnvironnvntalLaboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
Reliability:Klimischranking3. Study lacksdescriptionand recordsofthe method followed.There isno characterizationfthetestsubstance purity, and the study lacksanalyticaclonfirmationofthe amount offluorochemical proportioninthe solution.
REFERENCES
This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number C2282, 1985.
OTHER
Last changed: 6/26/00.
TEST SUBSTANCE:
3'ic6 PO
TEST DATE:
/0-Pg-
ANALYST:-
ENVIRONMENTAL LABORATORY
R BECKMAN'S MICROTOX SYSTEM
R
MICROTOX
RAW DATA
.213-.oo4 EAX -855*42
LR ia;tg3A4 Z2@29xn tLc+615&
SAMPLE CUVETTE
Bi
ci
B2
C2
B3 C3
B4 C4
B5 C5
FINAL ASSAY CONCENTRATION
BUNK COMMOL DUPLICATE
u p @c
loo v@181k
bv P Ic'a--te
16U
c
I
INITIAL READING
1(0)
90
5-MIN.
1(5)
9-3
FINAL RP)TDINGS
15-mill.
1(15)
30-MIN
1(30)
80
87
9
7S
24
-79
7,,@-
72
7R
6q
:9(a
7 t9 7 '57
SO
',17 71 S7
4 Ct St)
57
BECKMANOS MICROTOXR SYSTEM
MICROTOX
R DATA
REDUCTION
BLANK RATIOS*
FX- 310b lPe)-l.S:@.133-oEo-s.t+.r
Final Initial Stimi'ioolo3Ai .Topor\ (Lo-io,5-C-r)
BLANK CUVETTE 81 ci
*The blank ratios
5-MIN. LANK RA
93/,Io = o .q aJL 9;/S7 --0. 1-31
CCII
for BI and Cl should agree
I5-MIN.
BSCI O-OL-8
@1&7=0.647 1 with 0.02.
30-1I1N. RLANK RATTC DTFFPRFNCF
MEAN BLANK RATIOS
BI BK RATIO + Cl BK RATIO 2
5-MIN,
--9-R7
15-MTN-
0-943
NORMALIZED LIGHT LOSS (PEFFECT) - Mean Blank Ratio x Initial Sample Reading --I Final Sample Reading
SAMPLE CUVETTE
B2 0 C2
3
3
4
4
Vy@
5
5 t, c@o r"
5-MIN.r -0.018
0.62.4
0. co 11 0.022, o. i a &t
o. 13L
0. @30 1 0. a7.3
15-MIN. r -0-011
-0. c I @k 0.0 a. coal 0. 15;L
6 -lal6
o. gq Cl o -a 164
30--MIN. V-
Z LIGHT LOSS
AMPLE CUVETTE
25M P' 2
3 44[
4L 5 5 /L-)Co
2.18
T6 - r)a
t' X 100 I+r
5-MIN. Z
0.4 2.,g
1 19,
EFFECTIVE CONCENTRATIONS
(EC's) MG/L
15-MIN. %,4
9 1.'3
13 It
30--MIN. % A
EC@S
EC20
C50 (95 c80
5-MIN.
779
nfid. Limits)
1766 857-
3@ q 1)
15-MIN.
30--MIN.