Document gE0qzbyx9rLyqRVqErxdp73oV

ARZ2G -00%6 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G. DAPHNIA) TEST SUBSTANCE Identity: PFeCr-f9l5u.oro(o1c-tOacnteasnuelsfuolnfaotnei;c macaiyd,a1l,s1o,b2,e2r3e,f3e.r4r,e4d,5t,o5a6,s6,P7F,O7,S8,o8r,8heptadecafluoro-, potassium salt, CAS # 2795-393) Remarks field: The test from 3M lot number 217. substance is Sample was a white stored powder. Sample was taken under ambient conditions pNrMioRr taontdesetilnegm.enPturailtyandaeltyesremsinteedchtnoiqbuees9.0.49% by LC/MS, 'H-HMR, 'F- METHOD _ Method: OECD 202 and OPPTS 850.1010 Test type: Static acute GLP: Yes Year Completed: Study completed Species: Daphnia magna 1999. Report completed 2000 Analytical monitoring: PFOS measured at 0, 24, 48-hours Santaaltyissitsi,camlomveitnhgoadvse:raEgCesomevatlhuoeds calculated, or binomial when possible, probability with by probit non-linear interpolation using the Test daphnid source: computer software of C.E. Stephan Obtained from cultures maintained by Wildlife wInatservneartiifoineadlbLytdt.,heEAasctaodn,emMyD.of NIdaetnutriaflicaStciioennocfest,hePhoirilgaidneallphbirao,odPAs.tock Test daphnid age at study initiation: < 24-hours Test conditions Dilution water: 0.45 um filtered well water Diimlmuetdiioantwaetleyrprcehceemdiisntgryth(edutersitn)g: the 4-week period hardness: alkalinity: 113728 ((112786--113768)) mmgg//LLaass CCaaCCOO,3 PH: 83(8.2-8.3) ToC: <1.0mglL Conductivity: 313 (310-315) ymhosicm CalMg ratio: 35/13.5 NalK ratio: 21.3/6.62 3Li5g9htluixn.g:PChooltoopretroinoedo5f01f6l-uhooruersscelnitghtl,ig8ht-sh,oiunrtsendsairtky awpitphroaxi3m0a-tely minute transition period. 000682 wSatoscpkraepnadretedstinsdoilluuttiioonnwsaptrerepaatr9a1timogn/:L.A Iptrwiamsarymisxteodckfosrol~u1t9i.o5n phroouprosrtpiroinoarltloy udsileu.teAdftweirthmidxiiluntgi,onthweatperrimtaorpyrsetpoacrkewtahesfour additional test concentrations. Al test solutions appeared clear and colorless. tEexstpossoluurtieonv.esTshelesa:p2p5r0oxmimLatpleasdteipctbheoafketersst csoonltuatiionninwga2s460.4mLcmo.f Number of replicates: two Number of daphnids per replicate: ten Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 -- 48 hours): 86-89 mg (control exposure) 869.1 mg/L (91 mg/L exposure) PH range (0 - 48 hours) 82-85 (control exposure) 85-86 (91 mg/L exposure) Test temperature range (0 - 48 hours) 19.5-20.2C (control exposure) 19.3-20.1C (91 mg/L exposure) Element basis: mortality and immobilization Method of calculating mean measured concentrations: arithmetic mean RESULTS MNoemaisnuarledcocnocnecnetnrtartaitoinosn:s:Bk<LcoOnQt,rol1,1,122,0,203,3,335,6,559,1 9m1gm/gL/L Element value: 24-hour EC = 82 (81-83) mg/L 24-hour ECso = >31 mg/L (C.I. not calculable) 24-hour EC = >91 mg/L (C.1. not calculable) 48-hour EC1o = 53 (<11->91) mg/L 48-hour ECso = 61 (33-91) mg/L 48-hour EC = 63 (<11->91) mg/L All element values based on mean measured concentrations wStearteisctailccaullEavtaedluwahtieonn:poTshsieblEeCbsy vparlobuiets aannadlys9i5s,%tchoenmfoidveinncgeaivneterravgaels cmoemtphuotdeorrsboifntowmairaelopfrCob.aEb.ilSitteypwhiatnh. nTonh-eliEnCea1ro ianntderEpoCloaotivoanluuseisngwetrhee calculated were less twhhanentwpooscsoinbcleentursaitnigontshewiBtrhucpear-tViealrsmtoerteaglimteytohroidmmboebcilaiutsye. there 000683 Analytical Methodology: Analyses of test solutions were performed at Wildife International Ltd. using high performance liquid chromatography with mass spectrometric detection (HPLC/MS). When determining the concentration of the test substance in the test solutions, the same and most prominent peak response for perfluorooctanesulfonate was used. No attempt was made to quantify on the basis of individual isomeric components. The LOQ (limit of quantitation) was 4.58 mg/L in this study. The mean percent recovery of matrix fortifications analyzed concurrently during sample analysis was 96.2. Samples collected at test initiation had measured values from 85.5 to 112% of nominal. Measured values for samples taken at 24-hours ranged from 92.2 to 115% of nominal. Measured values for samples taken at 48-hours ranged from 91.6 to 106% of nominal. Summary of analytical chemistry data: Concapnvyaion, [and 45ers, Respacively mol|CaMncoasnrraeidon,| Nomina [ipmecm | meaco | aco |.| [2[oswsnsusooue[uwe|| [2 [oewmen|cn| ewws| [on [sosssm|mm| mnwe| [w [cosvomeom|mw u|| Lo lowoo|mom[mwe] Biological observations after 48-hours: Daphnids in the negative control, the 11 and the 20 mg/L treatments appeared healthy and normal throughout the test with no mortality, immobility or overt clinical signs of toxicity. Five percent moriality was observed at 48-hours in the negative control. The effects noted in this study were mortality; no immobilization was noted at any test concentration. a eeTe Cumulative percent mortality: TostConc.mol| 2hows| 4shous [geo | 0 | 5 | [=[oTo | [Ce[oo [ Lo[ow |w | 000684 Control response: satisfactory CONCLUSIONS The potassium perfluorooctanesulfonate 48-hour ECSO for Daphnia magna was determined to be 61 mg/L with a 95% confidence interval of 33.91 mg/L. The 48-hour noimmobilization and no observed effect concentration was 33 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 1 REFERENCES _ This study was conducted at Wildiffe International, Ltd. Easton, MD at the request of the 3M Company. OTHER Last changed: 5/3/00 000685