Document gDoRnkM0XqRr5ZzB2dgDqYMdG

TRADE SECRET AR226-3179 DuPont-6818 S tudy Title H-24616: Static, Acute, 96-Hour Screening Test to Pimephales prom elas Laboratory Project ID: DuPont-6818 A u t h o r : Daniel J. Peterson, B.S. S t u d y C o m p l e t e d o n : July 11,2001 P e r f o r m in g L a b o r a t o r y : E.I. du Pont de Nemours and Company Haskell Laboratory for Health and Environmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 Company Sanitized. Does net contain TSCA CBI - 1- H-24616: Static, Acute, 96-Hour Screening Test to Pimephalespromelas DuPont-6818 C E R T IF IC A T IO N We, the undersigned, declare that this report provides an accurate evaluation o f data obtained from this study. W ork Completed by: c'f)aniel J. Peterson, B.S. Associate Scientist / / - J~u f y Date Issued by Study D irector: . Robert A. Hoke, PhD . Senior Research Ecotoxicologist / / x / Ct/y c2O O / Date Ccmoany Sanitized. Does not contain JSCA CBi H-24616: Static, Acute, 96-Hour Screening Test to Pimephalespromelas STUDY INFORMATION Substance T e s t e d : ^ |^ P H ^ ^ S v n o n v m s/C o d e ^ ^ ^ H ^ ^ ^ ^ ^ ^ ^ ^ Haskell Number: 24616 Purity; Known Impurities: Physical Characteristics: Ooaaue tan liauid Snonsor: E. L du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. Study Initiated/Comnleted: June 25.2001 / July 11.2001 In-Life Initiated/Completed: June 25.2001 / June 29.2001 DuPont-6818 Company Sanitized. Does not contain TSCA CBI -3 - H-24616: Static, Acute, 96-Hour Screening Test to Pimephalespromelas DuPont-6818 SUMMARY The acute toxicity o f H-24616 to the fathead minnow, Pimephales promelas, was determined in an unaerated, 96-hour, static test. The study was conducted with 4 concentrations o f H-24616 and a dilution water control at a mean temperature o f 20.7C (range o f 20.7-20.8C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control and 1,10, and 100 mg/L test concentrations wore clear and colorless at test start. The 1000 mg/L concentration was cloudy at test start. All water quality parameters were within acceptable limits during the exposure. Exposure o f fathead minnows to the water control and nominal H-24616 concentrations o f 1,10,100, and 1000 mg/L resulted in 0 ,0 ,0 ,0 , and 100% mortality, respectively, at the end of 96 hours. No mortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 100 mg/L. The lowest nominal concentration causing 100% mortality at test end was 1000 mg/L. Nominal H-24616 concentrations were used for calculation o f LC50 values. The results are summarized as follows: Nominal concentrations o f H-24616, mg/L 96-hour LC50 (95% confidence interval) for H-24616, based on nominal concentrations, mg/La dilution water control, 1,10,100, and 1000 316 (100 to 1000) H-24616 exhibited low concern for aquatic hazardb in an unaerated, 96-hour, static, acute test using the fathead minnow, Pimephales promelas. * Stephan, C.E. (1997). Methods for calculating an LCsa- Aquatic Toxicology and Hazard Evaluation, Proceedings o fthe First Annual Symposium on Aquatic Toxicology, ASTM Special Publication 634 (F I. Mayer and J.L. Hamelink, Eds.), PP 65-84. Philadelphia. b Smrchek, J., Clements, R_, Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation of data. Environmental Toxicology and RiskAssessment, ASTM ST P 1179. (W.G. Tandis, J.S. Hughes, and M A Lewis, Eds.), pp 22-39. American Society for Jesting and Materials, Philadelphia, PA. Company Sanitized. Does not contain TSCA CB1 -4 - H-24616: Static, Acute, 96-Hour Screening Test to Pimephalespromelas DuPont-6818 TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal H-24616 Concentration (mg/L) Test Initiation (0 hours) Dissolved Oxygen (mg/L) pH Test Completion (96 hours) Dissolved Oxygen (mg/L) pH W ater Control 9.0 7.4 7.7 7.4 1 9.0 7.3 6.9 7.3 10 9.0 7.3 7.2 7.3 100 9.0 7.2 7.3 7.3 1000 8.9 7.2 7.9s 7.2a a Measurement taken at 24 hours due to total mortality TABLE2 MORTALITY OF Pimephalespromelas IN AN UNAERATED, STATIC, ACUTE, 96-HOUR TEST WITH H-24616 Nominal H-24616 Concentration (mg/L) Water Control 1 10 100 1000 24 Hours 0/10 0/10 0/10 0/10 10/10 Number Dead / Number at Study Start 48 Hours 72 Hours 0/10 0/10 0/10 0/10 10/10 0/10 0/10 0/10 0/10 10/10 96 Hours 0/10 0/10 0/10 0/10 10/10 Company Sanitized. Does not contain TSCA CBI -5 -