Document gDke7bp46875GLdQNL63ygBDV

Cin c in n a ti, Ohio June 20, 1955 Dr* 0 B* Willoughby Chemloal Department Experimental Station B* I. duPont do Wamours and Co* Wilmington, Delaware Dear Dr* Willoughby I fool that I owe you an apology for not batring at least acknowledged tha receipt of your letter of January 22nd long before thle* I hare your last letter written May 29th* During the past two weeks I hare been so busy with calculations it was impossible for me to write s suitable answer to your letter* It was very con siderate of you to forward the details of the bismuth separation prior to the publication of your paper* In looking through my file I find that I have lost the only copy of my letter to you dated Jaraxary 7th* If you still have this letter, would it be convenient for you to send mm a copy? My thanks in advance* , You ask if we are etlll interested in the collaborative work* In spite of our apparent disinterest I must say that It is only the press of many other things which prevents us from working on this problem* We hope that you will continue to hold a place for the samples we hope to send you as soon as a favorable opportunity presents it self* We have reoently received two reprints of articles appearing In Angew* Chera* during this year* It is my impression that these articles may Interest you, so that I send the titles in case you have not encountered them In your search of the literature* *Determination, of Minute Quantities of Lead by Electrolysis0 Angew* Chera* Vol* IpB pg,,2*}9 1955 Dr* Albert Peeke, and Dr* Herbert Muller of the Institute of Hygiene (Director-Prof. Dr. P* Schmidt) Martin Luther Universlty Halle - Wittenberg This paper is an answer to a criticism of the method by B* Behrens who particularly attacked the electrolysis step as yielding incomplete separation* - 2 Dr. wlllou bby **?Bting by Mean of a Radio-aotivity Method, the Quantitative Analysis of Minute Quantities of Lead as Carried out by P* Schmidt and Co.Worker* Angew. Che. Vol. 1*B pg2ol, 1955 Dr. vr. Measerscbmldt and Dr. 0. Partler of the Institute of Physios (Dlreotor* Prof. Dr. 0 Hoffmann) Martin Luther University Halle * Wittenberg This paper endeavors to show the completeness of the anodie deposition of lead during electrolysis by measuring the Oamma ray emanation of the daughter eubstance Thorton C" obtci ned from Thorium B the radio aetlvo Isotope of lead. They stress the advantage of the measurement of the Qsnng ray emanation over that of the Beta ray emanation. Their eonoluslon le that aa little aa 0 .0 3 mgs* Is ooapletely deposited during electrolysis. We have occupied a portion of our time In trying to establish the magnitude of the very small blanks encountered. In the preparation of smaples for spectrographto analysis. At this writing we do not eare to make a state ment as to the results obtained. Mr. Cholak has tested the graphite electrodes you so kindly sent and found then very similar to sene special graphite electrodes we have obtained from Motional Carbon Company at exactly the same cost. Tbs graphite electrodes used routinely fail to show either the sensitive lead or bismuth lines. Their oost in thousand lota 5/I0 * * 12" is 7 cents each. The bismuth nitrate we have used in prepara tion of bismuth solutions does not oont&ln sufficient lead to give a speotrographle test. At present the bismuth In ternal standard la a concentration of 1 mg. per 100 o.o. of solution as 'randy for the arc. The sail amount of lead capable of giving the line used in quantitation is 0.000002 mgs. on the arc. Hiring quantitation 0.002 mgs. of bismuth Is present on the arc. This amount of bismuth Is equivalent to approximately .001*7 mgs Bl(K0a)..5%0. We ean calculate that the lead concentration of the bismuth salt must be over O.OOl^e before the lead line will appear. Concerning the use of blood findings as a diagnostic mofisuro* I most repeat that the experience we have had does not permit us to rely solely on a blood analysis. I do not think our data sufficient to answer your question concerning the lead concentrations in blood when the patient has an acute attack of plumblorn. The picture In the case of chronic plumblam is certainly nothing so definite that one can rely on blood findings alone. Usually when the blood lead exceeds 0 .15 mgs./100 o.o. the urinary lead Is suffi ciently above the normal range and clinical symptoms appear which make it possible to diagnose the case. c' 5 Dr W illo u gh b y Th o r* lo no dots a v a i l a b l e on th e r e l a t i o n betw een lo a d in tho b lo o d and le a d In th e bone un der va rio u s c o n d i t i o n s Yo u a r e o f c o u r s e f a m i l i a r w i t h Dr Au b *s t h e o r y co n ce rn in g th e n o b llla a tia n and d e p o s itio n o f le a d b u t ho h a s n o t p re s e n te d a n y d a ta w h ich w ould re w e al s u ch r e la t i o n * Mr Ch o l a k an d I w e re v e r y much i n t e r e s t e d i n y o u r e x a m i n a t i o n o f t h e d a t a a n d y o u r r e s u l t i n g o c e e n e n t. We agre e th at d i s t i l l e d w ate r adds wary l i t t l e lead our s p e c tro g r aphis a n a lys e s show our do uble d i s t i ll e d w ater to o o n taln l o s e th a n 0 .0 0 2 m g a / l i t e r We d o n o t t h i n k t h a t th o p o s i t i v e fin d in g s in sam ples 2 *3 ,5 and 7 a re duo to co n tam in atio n Wo h a v e n e w e r e n c o u n t e r e d a b l o o d s a m p le w hich d id n o t o o n taln le ad Q u a lita tiv e an alys e s on sam ples d e p o s ite d d i r e c t l y on e le c tr o d e s and in vo lv in g no tre atm e n t a l w a y s s ho w l e a d We w i s h t o d o f u r t h e r w o rk o n t h i s b e f o r e we o f f e r a n y p o s i t i v e e x p la n a tio n At t h i s tim e we h ave fo u bd one fa o to r abo u t th e q u a n tita tio n which eo u ld e a s i ly a c c o u n t f o r t h e v a r i a t i o n fro m .00I4. t o 006 b u t n o t f o r t h e 0 00 8 found in sam ple 5* I do n o t know i f we h a ve co m p lie d w i t h yo u r r e q u e s t f o r s r e p r i n t o f U r Ch o l a k *s p a p e r We a r e s e n d i n g one under separate cover Th an k yo u f o r y o u r c o n tin u e d i n t e r e s t e n d h e arty co o peratio n . Sin ce re ly, KTi l e F r e d e r i c k 'fham&xm