Document gDgqaz1ZVJMZD5jXYbLGvwrJe
AR226-2531
TO: B. WONG PPD Parkersburg, WV
.
TABLE IX
.
Comparison of Cg/GC and Wlckbnld Torch Analyses or Rat Blood Samples W
PEAL So80-6/461 80-67462 80-67752 80-67756 80-67481 80-67460 80-67484
Sacrifice-Tube 1-6 1-7 2-18 3-18 -16 1-2
Test blank
GC Analysis, C? 3, Wg P/g bleed
Vickbold Torch Analysis,
Total Fluoride, Mg F/g blood
UncorTectad
x 170.83(W
7.7 6.9 8.3
13.1
. 10.5
12.7
11.4
9.5 ' 11.4
8-6 9.0 10.8
1.3 1- J.
' 1.5 *r
2.2 1.3
<0.007 (none detected above reagent background)
0* 76
0.92
(a) Analyses or. durlicate sornioni- or the lyorthilirec bines saap >. Cl analyses
as descritte in Table 111, 11 '
'5- Si . "iokbolc Tir".
E, Kisse, Jackson lab. II -il/C-l-"' '31, Notebook -eierecre 59
(b) Kecoverv or rerilucroccianci^ aedo m r*ie Aickbo_r Tc-ir: currently `'832, as d a t s m n e d in recent v-ork by E. Kiss
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TABLE I I I
(a b
Concentration o f 1'e rflu o fo o ctan o ate in Rat Blood S an p lea, GC BcCernltiatlon
S a c r ific e l. 0 Days
2, 14 Bays
3, 28 Days
, 4, 42 Days
lit T fcon troll
Tulie i
iC n ]. PK F / g
Croup I I (1
__ ,
Tube i tC n ], Mg F /g
1
0 .6 8
6
7.7
2 1*1 3 21.
7 13. 8 17.
4 3.3 9 20.
5
0 .5 3
10
9 .4
Ave,
5 ,3 1.4 e x c .l '
13 .
. ? rouP. I 11
Tube 1
IC ft], Mg F f g
U 53
12 28 13 37
14 61 15 57
47
3 0.30 4 0.43 5 0,1.2
Ave.
3 4 5
Ava
0 .2 8
0.086 o .n . o .n
0.10
8
9 10
0 .9 7 1 .5 0.84
1 .2
13
14 15
4 .2 4 .6 2 .6
3 .0
1 0.010 2 0.037 \ 0,058 4 0.038 5 0.017
i1
Group IV (80 m g/3)
Tube i
*7 8
16 17 18 19 20
133 124
89 86 no
108
18 19 20
18 19 20
j 16 I 17
18 19
11 10
8 .5
10
8 .6 5 .8 6 .9
7.1
1-3 3 .6 1 .2 1.1
1 .8
5, 84 Days
J 2 3 4
Ave.
0 .0 2 9 < .o o ; (c) 0,021 <.CfO? <c)
0 .0 ]5
1
1
\ 13
: 14 i1 15 I
0 .1 4 i-3 1 .1
0 ,8 4
I1 lszsoozra
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TABLE III (con't)
(a) Procedure as described in the method "Detarndtiation of Perfluorooctanoic Acid in Blood Gas, Chromatographic Kachod", 1/2/81, using Che capillary column GC analysis and perxluoro-n-octanoic acid as calibration standard. Lower Un i t for quantitaclon 0.007 ppm F. Estimated uncartrinity +10Z relative standard deviation over most of the range, somewhat higher at Che lowest concentrations*
(b) For the six "test blank" samples listed as analyzed in Table I, fCsl <0.007 ppta F (none detected above reagent background). (PRA1 Nos. 80-60368-69, 80-60372-75)
(c) Values of 0.005 (sample 5-2) and 0.003 (sample 5-4) were used tc calculate the average, but quantitation cannot really be done at these levels, and it is questionable whether 5-4 contained any Cg above background.
(
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TABLE IV Percent Straight-Chain Perfiporooctanoatc In Rat: Blood Samples (a )
Sacrifice
Croup I (control) Group II (1 isg/m^) Group III (8 tag/m^) Gtoup IV (80 tmt/nP) Tube # Percent Tube t Percent Tube # Percent Tube P Percent
1, 1 85 6 82 11 89 16 88
0 Days
2
84
7 80
12 92
17 88
3 96 8 85 13 90 18 87
4 91 9 88 14 85 19 84
5 86 10 81 15 86 20 86
2, 3 14 Says 4
;s
93 92 91 .
.
!.
!
i 18 19 20
93 92 91
3, IS Days
3 4
5
not - 8
cal-
9
enlaced (b) 10
93 : 13 91 . 14 97 15
96 ! 18 . 94
95 : 19
92
96 : 20
93
4, 42 Gaya
1 2
3 4 5
5, 84 Days
1 2
'3 4
not
cal-
culated(b)
\1 not. calculated(b)
!
_
*_ ' ..
-
! 16 17
. 18 - 19
94 96 95 94
l
! 13 'V97
, 14
94
15 93
(a) Calculated as described in the method, for peak #3 in the group of five Cg isomer peaks seen in Figure 3b
The same calculation for spiked blood standards CVLQ ppm F) gave average values
of 74* straight-chain for FC-143, 94X for perfluoro-n-Octanoic acid.
.
(b) A calculation of branched iaomer concentrations was cot atcampted for samples with {Cgl^O.l ppm, due to the small sire of the secondardy peaks and interference
from the reagents.
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Figure 1 - Comparison of Cg/GC and Wickbold Torch Analyses of fisc Blood Samples
Data from Table II, using Torch values corrected for 33% recovery of perfluOrooctanolc acid Linear Least Squares Fit: Torch 0.90 * 0.9S (CC)
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Gvj ppm Cg as F
04:4
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PIjur 2 - Concentration of Cg In Rat Blood SjubrIm yj - F.accv*ry Tl#a
Data from Tabi I I I , Avarage Value* by GC
..
A - Group IV lo g c - 1 .9 - 0.039 t (to 42 day ota?)
Group I I I lo g C - 1.7 - 0.039 t
.,
O - Croup I I lo g C * 1.1 - 0.038 t
'
X - Croup I
log C * 0.078 - 0.038 6(to 43day*o n ly, ta a p la 3-20 excluded/
^ ^ m * * * * ? * TM * - ........RJ2005291
Recovery Tim a, Day ,
045
' _
- <5 -
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