Document gD50KB2Jw0RwV3EgwRw5bX0DL
Visto Chemical Company
lake Charles Chemical Plant Old Spanish Trail, P.O. Box 727
VSfestlake, Louisiana 70669 Phone (318) 494-5140
CERTIFIED--RETURN RECEIPT REQUESTED #P241 411 248
LJU /3
1990
Ms. Evelyn Rosoborough (6W-PS) Permit Branch Water Management Division EPA Region 6 1445 Ross Avenue Dallas, Texas 75202-2733
Re:
Section 308 Letter of June 9, 1989 Requiring Chronic Biotoxicity Testing NPDES Permit No. LA000336
VIS1A
Dear Ms. Rosoborough:
Please find enclosed the summary reports, Table
and the formal reports for the monthly sampling
initiated May- 1-4-, 1990. / A-
I data, for May-
r
If you have any questions concerning this data, please
contact Paul Fetzer at (318)494-5067.
Sincerely,
M. G. Hayes Director of Environmental Control Lake Charles Chemical Complex
CUH 000<>.1.0361
PERMITTEE: PERMIT NO: OUTFALL NO:
ENCLOSURE 1 (PAGE 5 OF 11)
TABLE 1 BIOMONITORING REPORTING CERIODAPHNIA DUBIA SURVIVAL AND REPRODUCTION
Date composite collected:
#1: FROM 0-700 c - iv - o #2: FROM 0 7o 0 6 ' JZk - t*90 #3: FROM o9y0 (, - / Y - f5 ?o
Date test initiated: C - /7 - t 7 *T 0
Dilution water used: Receiving water
^
TO ^?oo
o
TO <s 9 o <y d> ' * 5 - / 5 9 0
TO
Reconstituted water
NUMBER OF YOUNG PRODUCED PER.FEMALE 0 7 DAYS
Percent effluent (%)
REP OX 52% 59% 67% 76% 86%
A
/j
B
73
c
33
D /?
E
F *f 37
G
7/ H
I
J
-GY3* CVl/-^
JZ 2**
0
O O G
0
0
0
c3
O O
G
0 0
O O a
0 0 0
0 0
G G0 O a0 O 00
O0 OO
O0 aO
a
0
0
0
0 cJ <D
0a
0a e> 0
O
O O
7 ((
S'
//
//
S'
//
0 6> S X-
0
3 S 7
y
o 3
<7
7
7 33
.?/
* coefficient of variation = standard deviation x 100/mean
CUH 000010362
PERMITTEE: PERMIT NO.OUTFALL NO.-
ENCLOSURE 1 (PAGE 6 OF 11)
TABLE 1
CERIODAPHNIA DUBIA SURVIVAL AND REPRODUCTION TEST
1. Dunnett's Procedure or Steel's Many-One Rank Test as appropriate:
Is the mean number of young produced per female significantly less (p=0.05) than the control's mean number of young female for the % effluent corresponding to (significant non-lethal effects):
a.) LOW FLOW OR CRITICAL DILUTION ( 76 %):
^ YES
_______ NO
PERCENT SURVIVAL
Percent Effluent
Time of Reading
0% 52%
59% 67% 76% 86%
2c,/0
24h 48h 7 day CV%*
f !_>
/v U
/oc/ fa o
fv 0
9o -
/ O <u 70 ' /o
/o o VO a
7 0
0
/<! 0 o o
/ o
/1 o
/t 0
90
ft, 0
* coefficient of variation = standard deviation X 100/mean
2. Fisher's Exact Test:
Is the mean survival at 7 days significantly less (p=0.05) than the mean control survival for the % effluent corresponding to (lethality):
a.) LOW FLOW OR CRITICAL DILUTION ( 76 %):
^ YES ______ NO
3. Enter percent effluent corresponding to each NOEL below and circle lowest
number:
a.) NOEL survival = Z,
% effluent
b.) NOEL reproduction = < /J
% effluent
4. If you answered NO to l.a) and 2.a) enter [P] otherwise enter tni_____ e______ "
CUH 000010363
PERMITTEE:
PERMIT NO: OUTFALL NO:
ENCLOSURE 1 (PAGE 7 OF 11)
TABLE 1
BIOMONITORING REPORTING
FATHEAD MINNOW LARVAE GROWTH AND SURVIVAL (Pimephsles promelas)
Date composite collected:
#1: FROM 07oo 6-/o - 9 0
#2: FROM o ?o ci A-o -
#3: FROM
C-/y- 90
TO 07Cio 6 -U -70
TO O 700
/* - ? 0
TO 0 700 6 - / s- - 9 6
Date test initiated:
6 - /a - 9 o
Dilution water used: Receiving water
Reconstituted water
DATA TABLE FOR GROWTH OF FATHEAD MINNOWS
Effluent Cone {%)
0% $2 i 59% 67% 76% 86% /3 %
%
Average Dry Weight
in milligrams in
replicate chambers
AB
C
D
MEAN DRY WEIGHT
(mg)
cv%*
Q.Yo o,</Y 6 . V/ o.J9 C.tfl.
<0-Z( 0 . i 2- <5. /'Cs CI. 3- ? O-Xo
0 0 . to 0-/0
0 { O
a
o . ol Q.tX
--
o.o r 0-/0 0-/o (3./
o.3Z 0 Vo
-- ,-- -- "5-3? Z" O. J 26, 3^
O.o J
O.OY --
o-5^
5" 3J
33
-- V //
* coefficient of variation = standard deviation x 100/mean
1. Dunnett's Procedure:
Is the mean dry weight (growth) at 7 days effluent significantly less (p-0.05) than the control's mean dry weight (growth) for the % effluent corresponding to (significant non-lethal effects):
a.) LOW FLOW OR CRITICAL DILUTION (76%):
j/ YES ______ NO
CWH 000010364
PERMITTEE: PERMIT NO: OUTFALL NO:
ENCLOSURE 1 (PAGE 8 OF 11)
Effluent Cone.(X)
0% 52% 59% 67% 76% 86% /5
TABLE 1
BIOMONITORING REPORTING FATHEAD MINNOW GROWTH AND SURVIVAL TEST
DATA TABLE FOR FATHEAD MINNOWS SURVIVAL
Percent Survival in Replicate Chambers AB C 0
MEAN PERCENT SURVIVAL
24h 48h 7 days
/W / LrXO /OX)
70 5o S'0
50 2o
70
O 90 4Q
3 <o
JO
O so 50
*so
7^0
0
c^o 70
/<m
O OtO
o
O
/CTO
Jroo
9 5-
-
?o
/H)
/hO
fW
/VO
95
S3
7a
fr /tr-o / rO
/" CRD y"" 33
7J /y o S5
cv%*
G /7 <>
3 Z. 7/
2. Dunnett's Procedure or Steel's Many-One Rank Test as appropriate:
Is the mean survival at 7 days significantly less (p=0.05) than the mean control survival for-the % effluent corresponding to:
a.) LOW FLOW OR CRITICAL DILUTION (76%):
^ YES
NO
3. Enter percent effluent corresponding to each NOEL below and circle lowest
number:
a.) NOEL survival = 4/3
% effluent
b.) NOEL reproduction - % effluent
4. If you answered NO to l.a) and 2.a) enter [P] otherwise enter
[F]:
CUH 000010365
PERMITTEE: PERMIT NO: OUTFALL NO:
ENCLOSURE 1 (PAGE 8 OF 11)
TABLE 1
BIOMONITORING REPORTING FATHEAD MINNOW GROWTH AND SURVIVAL TEST
DATA TABLE FOR FATHEAD MINNOWS SURVIVAL
Effluent Cone (%)
Percent Survival in
Replicate Chambers
AB c D
MEAN PERCENT SURVIVAL
24 h 48h 7 days CV%*
0%
/oo
f 0 /Oo Poo
/t?o
^00
52% 40 Po
40
/0 0
^/u '=
59%
Jo 3 0
30
/0
/* 0 0
3l b
67%
$0
30
"/o Cl
a 3 S'
76%
2. to 30
/3
Jo
^ a ci
/od J /
86%
P-D O
/o r 0
/ 0O
/dt)
/O
11> 7 0 X 0 APp 8* So 70
/* 0
/O 0
1 /o / <J 0
#0 ?S
* coefficient of variation = standard deviation x 100/mean
3 jr
"// V //
2. Dunnett's Procedure or Steel's Many-One Rank Test as appropriate:
Is the mean survival at 7 days significantly less (p=0.05) than the mean control survival for the % effluent corresponding to:
a.) LOW FLOW OR CRITICAL DILUTION (76%):
^ YES ______ NO
3. Enter percent effluent corresponding to each NOEL below and circle lowest
number:
a.) NOEL survival = S J
% effluent
b.) NOEL reproduction =
% effluent
4. If you answered NO to l.a) and 2.a) enter [P] otherwise enter
[F] _
( P
o/*4-~
t P& ^ bec-i-fV /l. 3./
f<^-t CUH 000010366
31
ANALYTICAL PROCEDURES
Refer to Tables 1, 2, 3, 4, 5, and 6 for the EPA-approved analytical and sample preservation procedures to be followed during this project.
DATA REDUCTION, VALIDATION AND REPORTING
For data reduction, validation and reporting, the contracted laboratories will be asked to supply a brief description for each major measurement parameter. The description should include: data reduction scheme planned for collected data; principle criteria to be used in validating data integrity from collection through final reporting; methods used to identify and treat outliers; and, the data flow reporting scheme from collection of raw data through storage of validated concentrations.
Quality control samples routinely included during analytical activities will be
used to set up a system of data validation, based on control limits established
for each analyte such that these limits will reflect the
confidence limit to
be obtained by using the results of all the quality control samples of that
analyte. All quality control samples are compared to the upper control limits
(UCL) and flagged if they exceed the limits.
If any quality control samples exceed the control limits, all data from the analytical run are examined in detail. If more than one quality control sample is out of limits or if any unusual instrument response is observed on the strip chart, all samples are reanalyzed. If only one out of three or four quality control samples is out of limits and all other parameters indicate that the analytical run is normal, the data are released.
CUH 000010367