Document g5yogM610pKD27Bgd4nn0MVa

FILE NAME: Chemical Abstracts (CHAB) DATE: 1958 DOC#: CHAB039 OCUMENT DESCRIPTION: Abstract Originally Published in 1955 by Policard 1958 , ,, ,v 12049 ; 'v ` ' IL--Biological Chemistry, B-- Methods 12050 (Nat]. Inst. Public Health, Utrecht, Netb.). Rec. trav. raphy is run 13-20 hrs. at - 8 with 85% HCOjH/AcOH/- chim. 74, 889-96(1955)(in English).--A method is described HjO (50/50/5, by vol.) as solvent. The sepd. compds. are whereby 20 mg. glycyrrhetinic acid (I)/24 hrs. urine can be identified.with Cu(OAc) and IFe(CN), or with Hg(OAc), detd. It is based on the fact that a soln. of I in 96% EtOH and diphenylcarbazone. Application of :he method to the has a max. absorption at 248 m/i. With a column of j isolation of the higher fatty acids of blood serum is described. Floridine earth I can be removed quantitatively from urine Morton Pader and freed from interfering substances. When up to 2.5 Isolation and identification of 1?- hydroxyestrone from the I was given orally in 24 hrs. it could not be detected in the urine of pregnant women. K. H. Loke, G. F. Marrian, urine. M. H. Neustadt W. S. Johnson, W. L. Meyer, and D. D. Cameron (Univ. Microscopic analysis of mineral dust in lung sections.' -Edinburgh, Scot.). Biochim. el Biophys. Acta 28, 214 A. Policard. Compl. rend, congr. soe. Savantes Paris el (1958)(in English); cf. C.A. 52, 2220.--The identification dipts., Sect. sci. 1955, 295-301.--Particles of soot, silica, of the Kober chromogen KC-6A as 18-hydroxyestrone was mica, kaolin, asbestos, and Fe oxide can be characterized confirmed by chem. comparison with synthetically prepd. by the use of microincineration technique and a polarizing y material. Morton Pader microscope. Braham Norwick Description of a dialyzer. Ren J. Girerd (Frank W. Clinical use of the simplified Porter-Silber method for the Homer Ltd., Montreal, Can.). Rev. can. biol. 16, 418-20 determination of the 17-hydroxycorticosteroids in plasma. (1957)(in English).--The unit is composed essentially of a Endre G6th, L. Lengyel, and C. Sively (Med. Dept. Margit coiled cellophane tube through which water flows and which, Hosp., Budapest). Acta Med. Acad. Sci. Hung. 11, 227-- - i n turn, is immersed in the liquid to be dialyzed. The lat 36(1958)(in English).--Minor modifications of the Porter ter is stirred with an elec, motor and stirrer placed in the Silber method ( C.A . 44, 9504c) are reported for the clinical axis of the coils. Though completely stainless, the unit is detn. of free 17-hydroxy corticosteroids in plasma. The best stored by rinsing, inverting, and allowing to dry. It normal range was found to be 11-26 -y/100 ml. *N. S. O. , was used mainly for protein prepns. and was found to be Colorimetric determination of fluorine in animal tissues very fast in operation due to the combination of a large with thorium-neothorin. Koichi Emi and Tadashi Hayami membrane surface and const, movement of the 2 liquids (Okayama Univ.). Nippon Kagaku Zasshi 78, 1532-4 always presenting max. difference in ionic strength. (1957) .--A 10 g. dry sample of animal tissue was mixed with _ A. Papineau-Couturc 1.5-2.0 g. of CaO (C.A. 52, 3007b) and ignited at 700-750. - Urinary osmolar concentration in the hydropnie state for 0.5-2 hrs. The F in the residue was distd. as fluosilicic as a measure of renal tubular function: a test for early renal acid and detd. photometrically with Th-neothorin (C.A. impairment. Martin N. Frank, Leonard S. Dreifus, Fred 51, 12747/i). The recovery of 1 mg. F through the entire Rarick, and Samuel Bellet (Univ. of Pennsylvania, Phila procedure was 98.9%. H. Onishi delphia). Am. J. Med. Sci. 233, 121-5(1357).--'The max. Enzymic determination of myoinositol. Arthur Weiss * urinary total solute concn., detd. during ihe hydropnie bach (Natl. Insts. of Health, Bethesda, Md.). Biochim. el state by the method of f.p. depression, showed .10 correlation Biophys. Acta 27, 608-11 (1958)(in English).--An inositol with urinary sp. gr. The lack of correlation was attributed dehyd" yrenase obtained from Aerobacler aerogenes grown on to the fact that various solutes, protein, sugar, temp., a medium comprising myoinositol (I) is used for the estn. _ pH, and other factors contribute differently to the sp. gr. and of y amts, of I. The method is based on the reduction o f" the osmotic pressure. Among 32 normal subjects, 27 pa diphosphopyridine nucleatide (DPN) by I in the presence tients with essential hypertension, 13 with diabetes meliitus, of an ext. of the cells. Under the assay conditions, max. and 7 with compensated rheumatic heart disease, those ca reduction of DPN by I was proportional to the I concn. pable of elaborating urinary concns. of 800 tm'liosmols/l. or At I concns. higher than however, this proportion 5 more were considered to have adequate renal function, those ality tended to fall off. This could be accounted for, in with 600-800 good function, those with 400-600 fair func part, by the accentuation of a DPNH oxidase system pres tion, and those with 400 or less, severe renal disease. Pa ent in the bacterial ext. A no. of cyclic ales, and sugars tients with essential hypertension and diabetes meliitus tested did not interfere with the assay. Morton Pader showed a decrease in concentrating function, as measured Isolation of cynodontin (l,4,5,8-tetrahydroxy-2-methyl-' " by this method, before alteration of other urinary function anthraqtiinone) from a saltant of Helminthosporium vic- tests or clinical suspicion of urinary disease. The test may toriae. Ross B. Pringle (Rockefeller Inst. Med. Research, serve as a graded quant, method of evaluating renal tubu New York, N .Y .). Biochim. el Biophys. Acla 2 8 ,198(1958) lar function, and in following the course of renal disease, (in English); cf. Phytopathology 47, 369(1957).--The pig f especially in patients with minimal tubular impairment. ment of washed, dried mycelium of the red-colored variant Marion Horn Peskin of H. victories was extd. with CHC1|. Red-bronze crys Sulfonamide blood-level determination in diabetics. tals, m. 274.5-5.0, were obtained from the ext. These were identified as l,4,5,8-tetrahydroxy-2-methy!anthraquinone, as detd. by analysis, chem. behavior, and lack of- - depression of the m.p. of the natural material when mixed with the synthetic compd. Morton Pader Preliminary fractionation of parathyroid extract with am monium sulfate. Samuel Friedman and Paul L. Munson (Harvard Univ.). Biochim. et Biophys. Acla 28, 204-5 ' (1958) (in English).--The hormonal activity of a hot, dil. acid ext. of bovine parathyroid tissue was coned, by a method involving step-wise pptn. with (NID^SO*. A con Arno Hanusch and Dictfried Jorke (Univ., Jena, Ger.). rsll. Forsch. 11, 451-61(1957).--Measurements of sulfon- amide and sugar blood levels in diabetics after oral adminis tration of l-sulfanilyl-3-butylurea (I) indicate that a sulfon amide threshold blood level exists, at which level the dia betic metabolism is kept in balance. This threshold varies according to renal function. The oral maintenance dose of I is the dose needed to hold the sulfonamide blood level within the threshold limits. The relation between oral dose of I and blood sulfonamide level does not appear to be a function of age. L. A. Pursglovc siderable portion of protein with low activity could be re-- Trichloroacetic acid and tolylsulfonic acids in deprotein- moved from the ext., resulting in material whose specific ation of human blood serum. Dimitine A. Iscescu, Petra activity was increased 3-4 fold. (The fractions were tested Ionescu, and Maria Macavei. Comun. acad. rep. populate for biol. activity by detn. of the scrum Ca 6 hrs. after sub Romtne 7, 845-51(1957).--Cl>CCO:H (I) and tolylsulfonic cutaneous injection into acutely parathyroidectomized acid (H) can vary the degree of the HjSO< digestion of pro- rats.) The fraction pptg. at 0.3-0.4 satn. was significantly 1 tein-free filtrate thus changing the analysis of the nonpro more potent than others obtained at different satns. Grad tein N. II, Se, CuSO<, and HjPO, enhance the digestion, ual addn. of alkali to a soln. of this fraction resulted in a yielding high values of nonprotein N. I and K*SO hinder ppt. which started to form at pH 5 and remained largely the digestion especially at 20% concn., thus yielding low undissolved to pH 10. At pH 6.5, the specific activity of. _ values of nonprotein N. Krikor L. Reizian the ppt. was significantly greater than that of the sol. por Trichloroacetic acid, metaphosphoric acid, and phospho- tion. The fraction was also studied by paper electrophore tungstic acid influence on digestion after deproteination of sis and sedimentation techniques. Morton Pader human blood serum. Dlmitrie A. Isacescu and Maria Two-dimensional paper chromatography of higher fatty . Macavei. Comun. acad. rep. populare Romtne 7, 941-7 acids. <t. Michalcc (Karlova Univ., Prague). Biochim. 1 (1957).--The chein. nature and the quantities used of the et Biophys. Acla 28, 212-13(1958)(in English).--In the 1st deproteinization agents in soln. influence the HsSO, di run, ascending chromatography is carried out with 93% gestion of the deprotcinized serum, thus changing the analy AcOH as mobile phase, developing 5 hrs. at 20. The sis of the total N level. ClaCCOjH and nhosphotungstic chromatogram is dried at 80-90. The 2nd chromatog acid hinder the digestion, leading to low N values, while