Document g2zYvy3z2360d0JaO8j505089

AR226 3278 60 DR UNOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE Earthworm (Eisenia fetida), Acute Toxicity Test in Artificial Soil ac. to OECD Guideline 207forTesting of Chemicals. (adopted April 04, 1984) (omy Sponsor DAKCIaNOrUuSTnRTIES LTD -- DupoKT Author Martina Noack Tost Facility DFRO.RUA.NNOGAECWKA-NLDATBEORBAIOTLOORGIIUEM K0a-th3o1-1P5a7ulSuasr-sStte.dt1 Laboratory Poploct ID SPtroujdeycet.oN.o. Study Plan-No. Page 10133 Date 2003.04-14 Company Sanilized. Doesnotcontain TSCA CAI = =o 3 Page 20133 Statement of GLP Compliance C me yy Som 100780 on he Nose, Sty Over frfo "or | IO...0 -Tipmpany S`saannittized. Doe" _ 3 so ie cud Toston A Statement of the Quality Assurance Unit 0 ---- 1] OECD No. 20 ort, 1980orTsing Crm ( cr --] wanna ~~ The study was verified as follows: rr TTT soso| morro Cn pSorvivoons TFooregrss mTotptneaeuvtscaluslocaomrityeee----ow--t--tty,Avo mts, Toisecrof so wh synnth ecofoGo erat --- DR.UNOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE -- fr Page 40133 `according to OECD Guideli"ne 207 ily Test in Ar # Sptruodyj-eNcot.o Personnel Involved `Stugy Director: (MBairotliongiastN)oack Technical Sta. Kirsten McVean PGewternadoMlaiknusKrastzig Barbara Wienker --_ Quality Assurance Unit: (GBuidolrougnistM)hrmann-Kalabokidis Deputy: Susanne Becker (Biologist) Hoad of Test Facility: Dr. Udo Noack (Biologist) [Company Sanltized. Does not contain TSCA C5 Table of Contents ~ Le List of Tables and Figures Kompeny Saniiized. 2023 not contain TSCA cm -- Li ) ge 60133 Table 10: Live WeightonDay 0 (100mg/kg) 24 | Figur2e: ConcentRrelaatiotnshiipoaftner1-4DEayfsoffEe xpocsutre 18 _ t Re.po OR.Ui NOACK-LABGRATORIUMe FOR ANGEWAN7DTE BIOLDr OGoIE rPageeaoTeonr T accorting 1o OECDGuideline 2Y 07 ] S1steoc.. 1 Summary G7T0uh.iedPeaOlcOiuInteGeOaNTfo).feo2cn0t7 aloDRrUmNEOAiGeK-nLeABOiRASTeOvRiIgMnyOwRorAeNdGeEtWrAmNiOnTeEdBaoeLeooGrIiEng1to3O1157 on Sarstedt, Germany from 2002-12113 12002-1227 ' daTiphpveliisdetddundnytawaoasutchorneepdluticceattteesds,aurnw.doeFrroerstetyastteiescdtcoponerdrgiatcniooinncssemnsatvrweairttaihoandn.urisaontliivoeinndtoufcao1nw4tereoiylgeha.onfdThs0.e3otnet1t.ei0n.m60w.as aii oi as described by to gukdelne. The study was conducted with the nominal conceniratins: 10-32 - 100 - 320 - 1000 mg/kg *). 240dt vn i mgt o dr whost rm oTfNexohprossiLugiCrnsei-f.civcaTaoalnhutieemcoworantcsaelingttryreaawttaiesorntshe1ae0nn00iihnketnhgeomcionrnaeclseunelttrseadttiioannmas cm1oe0nac-ne3nt2mr0oatmrigot/nkaogoflfa1f4t0e50r0%1m4aadfaekyrs1do4rfydwayasht ") wi 2] FEieel N/2A 0mNa al NMam |N9B 1: 7 7L gl1ve l 1 e 1E a g : . Como 11l1lE2e 1B|g azo wE l IAe GBTIA I e 1G R 11 R 11] ||] PP EE Figure 1: Course of Lva Waghts Tasman rat) * tse sca aoc NOVA. Dire' os, 4001) ~ `Tompany Saniizsd. Dodditotcoontain TRA CRE CA e TE. [mg/kg] [[T ooomntim | T |] | f| Ea [Fo |=]m =[wo r orr [or] `Company Sanitized. Does not contain TSCA CBI _ DRUNOAGKLABORATORIUM FUR ANGEWANDTE BIOLOGIE Report Pog 90133 according to OSEonCiDaGoulicdae),lineA2c0c7o To Vad SPtruodjyeNcot.o Tho no observed effect concentration (NOEC) ootwheesrtsoybmspetrovmesd eofffsecatnctoanceenftcreatiwoans(fLoOuEnCd)twoaes 5120M0ImGikoraestp(eceTrab1l4e d1)a.myosrtoaflelyx,poisuvreew.eiTghhet and 21R0e20s6u18l--t1s12-o(1f14t9h5se7h)roewafeneddretOnhEcteCtDtheesNtom.ewti2th0ho7d2(.i1C8h8se4ln)osri(otPaiavrceteta1a0n)md.ivdTaehlepdeL arcfcoororms fdetidnhgefftrooemtfh2ee0re0gn1uc-ie1de1fl-ii3on0mos2fo.DIN ISO Chioroacetamide was in the ange 15- 60 maiko. TiVnahletihdeaivtreyorfiaegtreaonilcoaesstoreefsqtubaiinroedmdbtahsystfohiefnatlghudeiadwieolnriinmvesewiteerstothefwuaclsofnitwlri.otlhPignerrtochuopnrtdemdquoirnraoeltdietvxyaclieuncotdhoef20c=o%n1.l0ro%l, group ES i zd. 5 ot DOSSTM contain' TSCACR! --~ mmm oR NOKGKLASORATORIN FOR ANGEWe ANTE Se oto0iE ~~ Sca " rin g is OEECrD Geurbaan c10o7 or WOON,7 4 aggee tout 33 Soeo 2 Characterisation Data of the Test Item resriren [--] ton umber oor cas Ju Gromit rractrcaion [4 oes) y) ~~ waters Appearance Yellowish solid wax 1T3S4T aa12152 dcoerrees.G,410280 v1p.o. n1e8?0uGPuopra,.2200002)0 taity ovate FR-- vo! gr wg! C ) S cant caT 17gon N Ss conde nites tres snr h recommend onstors. Recommended store Room tempera 20C), rotted fom moss, Storage st asin fakty Roan ampere, rotted fom mots an gh. Raton Aes 1 ha aan eins, a -a_neniisntgoinypromoter Name, bonand colo, The ettomand h formation concentstomwer roiod spor. --_ CompanySanitzsd. Dosenotcy?niain TSCACBY eT m RAITS, 3 Method ~ u rcore TSTO7S vor son ~ C[ o Tm o TTooovrem TToorn|Teo r Toowmes]] omens meagan wa oo | ww| | wo Ce TelsToeTole] Tr rt --~ , uny SanitiDozeesdno.t -_ DRUNOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE --~ ios Page o 1 130133 accordinvgmto O(EsConDiaGfuliide)l.inAec2ut0e7 Toxicity Test in Atfcil Sol J SPtruodjye-cNt TEST METHOD Roplicates Fpoorurcornetpoilcatlreesatwmeernot.used per concentration of the test fom and hNeumebaerrthawnodrmasge of `1T0heayduhlatdeabretehnwaocrcmlsim(awtiithsecdltoelatufmi)ciwaelrseoiulswedopdearyrsepplriciaoter fest 0st6ar0. gAt.start ofthe test, thoy had an individual ive weight of0.37 Test medium cAtofnisciisatledsoifltwheasfoulsleodwiansg dceosmcproinbeendtsb:y th test guideline. It -2100.5 %%kapoleiant(aKiaoldiniietde caonndtefnitne8ly4gr8o7u%nd)) 06.95%%qcuaalrtizumsacnadrb(osnaantdewi(tChaCpOasr)tce size of 50-200 ym: > 50 %) p~ m`aDlesmtiunreeracioznetdenwtatoefr2w7a%s oafdtdheedd(r1y0w8a0igmhLi)(taobogiuvte2a5n%ovoefratlhle dmaext.ermwianteedr ctahpeacdiatyy)beoffotrheeaspopll.icTahieondoeffintihteivteesmtoiistteumrbeycodnrtyionrgt,a vSaalmupeloefa5t.1405waCs ainndthreo-rweecioghmimnegn,dweadsr2a6ng%e. oTfh6e.0m+ea0s.u5red pH. Test container hhGloeasltseossdtistsuhhbeesystwr(aavtroeelfucrmooevme1rd.eryd5i)nwgi,wtohrpeerufsoerdataesd tpelsatstviecsfsielmst.oDpurreivnegnt Application technique wsTteeairstgt.htHToohmf twchoaenscsoeilnnltcroartpioornasteodfitnhteo ttehesatiefmiiaarlosroella2t4edhoprtihore (d0rytest Feeding -- seTtahhneedr..teAsTthrelettmehsetwsaolslevmweensittgohhcaekddsoovluoutltaiatoninzdewddaiossustmooilfxvteehddeiwniqtfuheatr1t0-z.m6seatqhnuydol,r-tbtzhuotyl tarheematsoaiidl.txqTouiaeerntszaurrsemanadohwooaufmsoqngucaearnttefozuolsulaysnmddiiasxtedrddibeiudnttiwoonatsohftthaheresitaeasmltefsfoiorml winit `each test concentration, solvent contol and control reatmant After 24 h the test substrate was added to the ost vessels No food was added tothe tost vessels during the toxicity tos, Company Sanitized.Dossnotcotain TSCA CB -_-- DR.UNOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE ort Page 14.0133 ~ `a;ccording to OECD Guidsfia)i,ne 207Acute Toxicity Test in Acie Sore7d SPtruojdeyc-tN-oN.of 2 Temperature Photoperiod Light intensity 18-22C 18h 400-800 1x TOFYPMEEAANSDURFERMEEQNUTESNCAYND OBSERVATIONS bTehfeormeaaxdiamptuamtiwoantoefrthhoeldeianrgtchawpoarcmisty(oafcttuhael s1o0il6w%a,sadcectoerrdimnignetdo 1SO DIS 1128-2 / Annex C). Al the start and at the end of the tdeestte,rpmHinaenddinmoeivsetruyretrceoanttmeenntt oafntdhecotnetsrtolsuubssitnrgatmeixweedresamples. of al ropiicates. Tthheedaliyvoefweaipgphlticoaftitohne aenadrtohnwodramys1w4.as measured individually on --~ Meoarrttalhiwtoy,rmbsehwaevrieourrecaonrddemdor7phaonldog1i4cadlaycshaanftgeersapopflitchaetion. TEST DESCRIPTION AconpcreenlitmriantairoynsNO(N1-0-G1LP0-0tes1t0w00asmgp/ekrgf).ormEfefdecwtisthon3mortality and `tChoentmreolanreplliviecawteeisgwhteroef ttehseteedarutnhdweorrmthsewsearmeeovcaolnudaitteido.ns as the. tost groups. pBearsfeodrmoendtwhiethrefsiuvletscoonfctehnotrpartailoinmsinfarroymte1s0t,ttoh1e0m0a0imngs/tkugdywiwtahs.a geometric factor of V10. Preparations: wAattifecrtiaol asobioluwta2s5p%roodfutcheedmaanxdimmouismtweanteedrwhiotlhddienmgicnaepraacliitzyedof the artifical soil. t was adjusted o a pH of 5.94 Day 2: Emoairstthewnoerdmstowaebroeutpl3a5ce%d oofntthheesaaritlifdicriyalwseoiighse(paadraapttealtyion). Day-1: "tTohret-tmesetthiyt-ebmuwtay-sethheearteadndtom5i0xedC,wiwtehig1h0egd qouuta,rtdzisssaondl.vedAfitner the solvent was quartz sand was volatized out of mixed with the atrhtefqiiuaalrtszoisland, the troated Start The treated test substrate was added to the test vessels. ~ Fang Senltized', DesHoottoofcontain TSCA CB! ee rrr DRUNOAGKLABORATORIUM e FOR ANGEWANDTE BOLOGIE A Revpert J Pag9e 150133 according to OECD Guideline 20A7 CE 4 Seveaen PiEnHadiravtinwdduoamrlalmlyssaiwnudraerpciwoanactseehneotdnwetnrheedesdcuehrtofearrcimeniaontfeedtdhvaitpnesgwtaptmeorol,edwdeuisgahnmopados cofoveearcehdrwoiahmpernotraatnedopnltasoticgrfoump. The tos vessels wore oay TErheasrapoeacrttetros,moIwrvetoraoallryttaawkneodnrmamuaetrpowhfeorlteaesritecepaslltavaceenddssoSenOltRaaenVdsOouLblsreefrnovaaefndcgeweei,h test subtle and tat vessels wore covered wih paforated in. Day 14 TSrheesapienrcdfitodtmuwrletoirlvtartywaiakengedhntmaouortfpo1hfo0ltohsoguirtcveiasvltovaresnsdwseablesshamaveniadosuuorrabelssos.rnsaendogewirnt.lh caondnrmlosiswteurree cmoossnurtoefdeeuvnseirtnyg epsotleHdosarmopsltemsens and of ns --- VALIDITY CRITERIA Tenhdeomfatrhteittoyt.nthe contol rou should not exceed 10% at the SThheouatvenroatgeexocesesdo2f0b%io.mass of the worms i the conto groups CsAtLaCiUsLTAITcIsOANNSD NOE, ive weights dASnioagtnloiyfmsiiicsnaneotdfiVifanreciroasmnnpccaeersainsidonntwhwoairtMmhtafhlevicwoeCniogthmetpaarrfoitusrpo4nusMidneagyssOhmwaeorfoe y oNDoUhrmEaNlToiStrym.aalnWldyhEeaqnnudulErqViuaanrligaVnaacreOinaTonecsWetaTwyoesrAtenwdeaorntesa<rso0tf..0V5a,-rviTaaoneeceeesstarfoere nwoererAeNcOaVrAedanodtDuUsAinNgETSTg'matsesitawtas 1.2.003.0(51T0h9e3-s19a67c) lSreee LnCeur-vveaslufeorsmnardiaclotnyidonce aGaolnncavueoasntaounnsosinnfgosrothfewasrferSenicme ienmewye.r3o0c0a0r(esdoo,ut voiraers os --_ Company Sanized. DosS notcontain TSCA!cat --_ S re OR HR OAATROTRFAT raTvoEOANERTEsIc, Me ojo eport Scting 1 O20D Gut-- e 37 --nN SeiePa16gofe33 CProrcnotatreeussaercaciotrdning0tSoWreweaie sc(1o5ro8r0),roCsaelotsoenofshe ThotGtuartlscrnhcos gfomecontol cu ot dotorminns TONEOF THESTUDY ToEEvaGTuOnNSeFsROM Th hy as cri ut om 2002.12.13 020021227. ne ToEEvSaTouNsopFoyROM ane Pp [-- Tahcrrepersi sadvgpaorcopy (5g) and am Arcs pTrhsialtnletidend5gwsistbortnod nmehirvsoonfoStt np ity or SiS nPtirravraaeroasrnpiopey varetrassdee1yo2ernaty1 sos pram om oatee n ams V MrioiSalvmakDa51a7ntndodois ggSatpDooswet!ann SAC -- TTDA RLNOAGW KLABORAm TORIUMe FOR ANGEe WANDTEe BIOLOGIEee -- Scokrormdng to OED Guide-- line 207 Page rors SProorjeic.to 4Results 41 Wortaty The percentage marti ater 7 and 14 days of a rplcates is given n Table 4 Talo 2 CEarrewpoersmMtoerrany in 4) aftr 7 and 4 Day ofExposure Nomina J wor | [2 Tony [a | 4 mw ~ savCeonwtcon||sst0 |[o0 e[]oooo e|a|o|To|ooT]e o fo lololololololololae 2 fo wo loffooflaolollooffool|ao]|oolloollso 2 wo f|oooof]oo|foo|loof]ww|o|fwo|lso|ln]]aws W = mean ave TeNxhopeossLiugCnreief-.icvTaanhltoumehoriwtgaahslsittnyltowsdatoseldosrecmoinnncaiebnnitt.hreattieosnteodf c1o0n0c0enmtraatkiorness1ul0ed3i2n0 mmagrtfyerof144 .days of .`Company sanDotsseatc.oontainTSCA cai -_-- DR.UNOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE Ciot Page `according to OECD GuideliJPACULSne 207Toxicity TestinATGHTSOM SPtruodyj-eNcot.o 1801 33 7 : 6 = . wn} - 2 |E oS s 1E 0 E 15 n 20 E e 25 n 30 35 ages. Figur2e: Concentration-EHfect Relationship after 14 Daysof Exposure `ECaomgpapnyanySantizgd. Does not contconat in TSCA CBI om SRSusOn--es s----Io --c--one oe i smcms macrt ear th dcottos n 0055 pi Company Sanitized. Dos5s5ffiottcontainTSCACBI ( ws| ecco sowm-- umeemorsacos `according to OECD Guideline 207 Study-No. ~ on | |p |e | em ew |. [ow ow | ow [| Cow Tose | os | w ] company Sez.Don et in TOGA - Cre raw gTrveorees sste 1 co ofprotveves AA NAN onEeS -: _ [Socmoe T|oaoenaJ[ovoemwJJooannor[[ooourmrTJToosomossamooen]||rw raa|wee]]] [ o [To emJT o[ooammJ[[ooomwo[[ooooo mwrfT[ooommmssaaonnm||| araw wriw||5ev] a]] [ E m TomToe wTomTomo son| onw |v] --_ "igimpiny Saniized, Dossnot contain TSCA --_ -- T DRUNOACKLAT BORATORIUM FOR ANGyEWA]NDR TE BIOLOGIE Page 220133 _ s ac>cording to OTr ECSDe GTuOTIidem liRneu2l0e7 Tone i Tost ASAd IN SPturdyoNy co 5 Validity Criteria TTCohhneedimatovireotrnaaslglefyorliontshtsehoevfcaboilntoirmodaolsfiostthnyuotheesitdcownnetarreoelaxgscrefoeouldplo1dw0si:d%noattetxhceoeondd2o0f%theatttohte and of the test, 6 Discussion and Conclusions (MToratballoit2y)was seen at th tot concentatons of 320 and 1000 molkg aftr 14 days of exposure 0cT.oh0me01p)ia.vreePwdaetithgoohlctooglwircaoasll sgstryaotmiupspttiodcuamlrsliynwsgeirtgehiefsi1ec4eanndtaayytsccroonfeceaexnspteorsadtuiraotencs(oA>nNc1Oe0nV0tAr,magtD/iUkognNN(=ET1aT0bT0l Mm5e)athkod,whpen< cNooncteensttrtateimonrsel=at3e2d mmgo/rktgi.y, pathological symploms and reduction in Ive weight wore seen at --_ TmFohirentaallliotwtye,hsetbHooidbgysheewrsetviegtdhetsetfaefndedcctooctnohcneecrnetsnrtyartmaiptolinoonwmist(hLwOnaEosCef)fofwuenatds(tfoNoObuEnedC3)t2oomfbagth1,e0t0esmtof/kesm with respect to aEfnfdecvtasdofPtahretre1f0)e.rance em 2-Chioroacetamide on earthworms show the method s sensitive 7 Literature / References (1) B16B2A6 (i1n9K6a4)n-sVteircfhaehrmeBnsovdoerns"ch(iLaCg:50.,To1x4izTiatgates)t,esBtraaumnsRcehgweonrwdurm Eisenia fetida (Savigny) (2) VBERBEG,USTG.A,VTFoisucrhenr,VEWR.LA(Ged,itJoornsa,.1952) Methoden der Wasseruntersuchungen Ba. (3) DIN ISO 1126-1 (APR 1897), WIRKLNGEN VON SCHADSTOFFEN AUF REGENWORMER. 4) AOEoCrD,G1u9i8de4line 207fo Testing of Chamicais: Earthworm, Acue Toxicy Test adopted (5) Weer, ., Grund dor bioogischen Statist, 9. Aug (1986). --~ iy SannDiozs ed.sin TSCAcat campany 8 Live Weight Data of the Earthworms [[=JoJsoeeJoosuora[osstefaourroJosseeofoosntofossst[oosse[[aossee[[oossse]]ssoarr [[rraeoorr[ovoms|] [+TouJosr[asoourfase oso [oss[ar]ose [ass] 510[oases] eeleelis fo oleslaloslonleeloefelon] ~ Elameee FTE] Frheahfontos easafoafoniceoTefloesa[oto] [Jososs ass ossooroso[ose[oss aur[ose] va ro wiz eos] [+ Jose]osoose [oaross [ossass[oss ass[ost|sve[v0]ose[ous] [200 Jao]osz] WEIGHT OF EARTHWORM No. [- [=] FEfoaeatoeafofreomtonotoar Tfolioneo [+Jessfousose Tossosm [oor[aes[oss[awe[os1]wz vo]o%s[00s] [Joseost [ox owe oseozods[oso]aso[oss]son[ro ast[aor] [200 [soos] ~ Sewpany sanitized. Coes not contain TSCA CRAY fron ortratrratoulss | [+Jos]oossee[oss [oso[ost oss] [2[esr[osTosTosoToso ass[aso[ose[05s |455[10050 [052] 530[10]ose [oe| [00s] [[2+TJoososJJoossso[oossss[[0a4s3o[oosss[oosues[[o0s4ss[[0o5s5s[[0o4s3s[[0o4sn| 551267[[100[aossse[[oaovrs]] [2Je0 o os7e] ~ eha =] LE [+[ese]o[s070[[05a5[soe2[ceo]055[aso]oso] [= JossJorom[057[oo[ous[our[ass[os[ase] 522[10]saJoos] [2[ose]osz[oss[ost ods[osv[wen[aanams05s|516[v0]asa[002] [ToseJossossos2[our[os[ose oss[ans [051]507[10osJoos] [207Teo[osz] nSLL LE Table 11: Live Weight on Day 0 (320 mglkg) [] WEIGHT g OF EARTHWORM NO. [T=] [Doso a [os ssosoe oss oss wz[oan [osvaov]o] [= [2 Jouroso[oso oso[os[oss[oaross [oso[osor[os1 [ost[ose[ous[oss [054 [047 [051|516 | 0]0s2[oor] aso] 516[10]ose[00s] [+Toseossoar ossossose[our[oss[oss [os | 50 [10[ost[00s| [205 Jeo]ose] = S-- =i [] WEIGHT 5 OF EARTHWORM NO [-~[[=] [fess[esJeoesfoersfrTaolmonotmo[sotcoauofev]efraoo a [ro]oT] [2 [> Jose oss Joseoss Jostossfost astfoss[ose oss [ost [ose[ass ass[oso] a0[v0 [oso as[ass [ods]so [vo aso[ase] aso [ae [Jew]owTomomowowososm [osnou|ssoTroove [oon [ros [eoTose] [~] WEIGHT g OF EARTHWORM No, [T=] ~ [[JJeosseslofossmtofosureo[osue [[oososf[aosae[[oooatr[[ouoruorsseo[oas|55 [[rwoa]osst o[owss] [L2e JtebsteJlowerloearrl[oereolmesalsee[aoalre[oezoloesetl[weaaa]t3fe0]losoeone]] Flim TTT [JoseJouross[oseToss [oss [2 JoseJoseos [ost [oso[os [os [ass[oss ase[sr [052 [oss]soz[0[me [oar| woo[0]ose wr] ome] [[=ToJoseeto[osstoTiosm[Toosmsoosme o[omsso[osw[oasse[oossst [[oouss]] 571s0[Tsroo[oosset [[aouss]] ] primera EEE --[ - -- --_-- --] im A, SEER [~] WEIGHT g OF EARTHWORM NO. [=] ealoeTen] [oxiosos Tosi]vo [feomi] [Joao[ose [ossow[ust[ore[oseuse[sa Eor[misfmaseoe] [] WEIGHT g OF EARTHWORMNO [= [x] ~ [E=RJfEeecm[[C osaToasy sr[[o[ostso[ouwwJ[0uasars[[oow2 s[oasost[EaoXszeefEoaEsse]Ro2wE[EiwooEewtNooEarr]] (TatTossow[aorom[ase[ow[ass[ose a[osrsT[iooloesss]Tear] EEE [=] WEIGHT g OF EARTHWORM No, [-][[=] IC E20 yeEE Ey RP EX Closeesa[oscoss[os [our[oar [ouroa][ovissTTiooloouw]Tour ~ van Sule Docs ho conn TSCA GUL ~ FlerJTe [+ Jess fosTosaossoer [ous ass[oar as [oa]soo[70 ame[oor] [[>2J[oesatt]foosss[oosso[[ooiooToss[[ooss[[aasnse[[ooosrs[[aomas[[0o3a77]|5w5o0z[[v100[aasmoe[oowost| [+ JosJossoseeros[oseavroar[ous[oi] 90 [10ome[oe] [sr[eo ose] FlerPTeT 2 [JoseJos osrToss fosa[oar[~~~ 25|o [om[oor] [2Joseoa oso[os[oe [ozs||| | 2s|&[035 |aus] [2 Jossossozs[asifost|117 |+[0 ose[am] [JesJomomfosoTom] T=]|vas[s[ows]om] -- [20[zz[oss] ---- T ee ORNCAS CRASOFORRaiACETWAOOTT ERLUOMO ~ EE ----] .... a 9 Physical Data brie [[[oSeCmeaom{ a[S |oeseynmn0TTT Tseseena T ||n wsemrw aoi T| aa ma wie]a r]] ~ [o Como[|e oenTTosve |a oeee Ta oais] . CmToo se |wrTa] "Timp Senitized. Doss nol ~ e ORU.r NOACK-LABO-- RATORIUMr -- FUR ANGEWANDTE BI0Lr OGIE eet --_ [ ] Page290133, `according to OECD GuideliPneRE207 PoucityTest n AficiSaolild SPrtoujdoycNt-oN,o. 10 Data of the Reference Item 2e2a-0rC0th1hi-wo1or2ra1ma4sc..etTTahmheidsreteufidseyruresone.cdevatasessRtrewfaesErepnocerYfaormmedinatthethActuatts fToaxciictiytyfrToemst20in01A.r1i1.a3l0 tSooil with cTohnecernetfrearteinocnes:am was applled anco at es tart The study ws conducted wit the nominal 15-30-60 matkg The mortally fo the eferenco tom was determined af7tanrd 14 days in prcont (Table 22). Tabl 22: Reference Ham: Mortal of the Erthworms ator 7 and 14 Days --~ Ta wa tes [aam s[l a mw Cw oa]o |o0 o | 0][aso |o]ooo loo|]wo ]o|aos 20 50 | 100 | 100 | fs | 100 | so 100| |e 100 | [a2] 100 | 10000 100| 100 | |e | 100| 60 |oas 100 | s00 eTxhpeosluowreestwatsesdteedtacramnicneendirdaiiroenctrersoutmintgheinte1s0t0resmuosrftoable 6(104dmoGsye.) afer 14 days of ciTnohtneecrevmnatlarcnaatnilgoeintnhwaglafscooinmncet2nh1te.r8arte1ico2on5m.(m21e4nmdd/eLkdCgersa)enswgteaimsoafdtteehdteebrgymuipinrdeolbdiintteoasnba(elDy2Is3Ni.s5,15ms0ool1hk1ag2t6w6ti-ht1eh;maOocEaoCnnfDliedt2eh0na7cl)e (Tab 23 aFinndalolyirtheffceocntcsenwtarastifoonunwdihonboae1ff5ecmtg/(kNgOEaCf)trof14thdeayresfoefrexnpcoesufrlaem.with respect to mortally --~ ~ 1] 3 --_-- DR.UNOACK-LABORATORIUM FOR ANGEWANOTE BIeOLOeGIE Page 0013 accroirdniwnogr10 O(EElCseDn GuideliJOP08)ne 2Ac0ut7e Toxcty Rest ARNESON SPtruodjyeoc.t Taba 23: LC-Valuos afer 14d for 2-Chioroacatamide Lo-vaives| Goncentation | Confidence marvel mgt] more) [ee[we T7-- | me TTzema| Lee TT we = not dtemines Trohaecieiaornsthtwoomremcshainnitchealcosnttirmoullausndantdestneggraotuipve1p5hmogloftkagcischorweaecdtinoonrsmaafltbrephaacviionugrtwhitehm noonrmtahel substrate surface. Obvious pathological symptoms were observed at 30 mks. cTohnelwroolrgmrosuphsa.dTahnerienfdiovrieduaalllwwaeigihgthsotfwe0r.o90intoth0.6o0 recogamtmteesntdsatdartrainngael.l TcohnacemnetraantiIovneawnedights r= `across replicates for each concentration and control were calculated (Table 24). dDuurein0gtthheeftaecstt, tthheatwtehiegrhetwoafsthneoefaoratdhiwnogramsaldloicnrtehaesetdostinptehreiotdr.eTahtmeenpteracnedntcoonslsrolingIrvoeups wc3oe0nimcgahantitkcrgaatm(i>eon2t0o30%8)%ma(lTkinga.btlheeTh2co0on.ltoosls,otfoi6ve%wenitghhet wcaonscseinginriafilcoannt1n5 mhge/kegstaendd cuopncteon3t7ra%tioin the Tablo 26: Maan LossofLive Wolght Conciamngthagtion| Toguvtosma)rt [oom 100 | Tiesgtoarn)a 1 ous |-- 5[ao FsToe Coo Tow [om 9 | Tf -- po 5Tns7L Se rooerotaan ntosoeno>s2300%0%) scAce! --~ `Compan'sanitizedDoes not Tce [ o -- -- JRa Themes wi ate 4 oy. corcentions 5 an 30mg es emcompares oe Ee10 TH vesGomaoe oe moss Tai [[SCooar[[owmeJ|ooumr aoasr[[oowme oomm| |] ] [Co[oeJom[om|=Tow| u----] - 7ToWistar x ~ --OR UNOAGK-LABORATORIUM FOR ANGEWANDTEeBIOeLOGIE ~ Rezo according to OTEeaCnDia Gfuoiiddae)l,inAec2y0l7 Toxicly Test m Aficio S09] Page zor PSrtoujdeycNtoN.o,--] 11 Certificate of Test Item Analysis BE = Clariant --_ = bam ~ -- riT DRUNh OAGKLASORATORIUMe FOR ANGEWAN7DTE BIOLe OGIE ram accoring 1sOECD GuideliCneO20R7 OT Test in Arial So Spreoj.. 12 GLP Certificate of DR.U.NOAGK-LASORATORIUM LPBeschu einiguncn/s Sttamtoamnetsnrtm r uof OPLry ctCompliance S Emaa gE en RS R EpR s sn mn ornXroaRnLgaASOTTORyIN aa C3bS prTr.isske y ewcnaomaTORY en, Sm Se, --~ LE-- me---- 1araame eeamtaigcSosntavtvaecnrsraerae ipooSpnarteestre dOSrosSsiI en oAEE reer gvnreersin arn dnChonan 2 At5o C Senr otpw 3te 0ooS tet sgJn.o 1,201 ES EEmErme IetEr eRSr SoSraeEs s p SE o en ri s EEsmmmimms fm Eiirem > i4f o2o .5By), Ammasnns yes Froarl PY in TSCACa --_ company S27tized.Doss notete