Document g2rk7DMyQDz23vxmerRpoR1pN

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA) TEST SUBSTANCE Identity:Perfluorodecanesulfonataem,monium salt;may alsobe referred toas PFDS, FC-120,or3M Sample J2905-1.(1-Decanesulfonic acid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10heneicosafluoroa-m,monium saltC,AS # 67906-42-7) Remarks: Test sample isa mixtureofthetestsubstance inwaterand butylcellosolv(eapproximatel2y5% testsubstance,37.5% butyl cellosolvaend 37.5% water).Allvaluesreportedrelatetothismixture. No calculationwsere made to adjustfortheactualconcentratioonfthe testsubstance inthetestsample. Test sample was from 3M Lot164. METHOD Method: OECD 202. Test type: Staticacute 'GLP: No Year Completed: 1992 Species:Daphnia magna Analyticalmonitoring:pH, dissolvedoxygen,conductivittye,mperature. Test substance concentratiownas notmonitored. Statisticamlethods: ECso valuescalculatedusingBinomiaI/nonlinear interpolation Test daphnid source: In-houseculturesE,nvironSystems Division, Resource Analysts,IncorporatedH,ampton, NH Test daphnid age at study initiation<:24-hours Test conditions Dilutionwater:Aerated,filterendaturalwellwater Dilutionwater chemistry (from a representativesample): hardness: 166 mg/L as CaCO3 pH: 7.2 Conductivity: 540 pmhos/cm Stock and testsolutionspreparation:A primarystock solution was preparedindilutiownaterto yielda testsample concentration of1000 mg/L. Alltestsolutionwsere made by dilutintghe appropriateamount of stocksolutiownithdilutiownater. Exposure vessels:250 mL glassbeakers containing200 mL of testsolution. Number of replicates4: Number of daphnids per replicate5: Number of concentrations:fiveplusa negativecontrol Waterchemistrdyuringthestudy: Dissolveodxygenrange(0-48hours): 8.7- 9.3mg/L(contreoxlposure) 8.8- 9.1mg/L (100mg/Lexposure) pH range(0-4h8ours): 7.2- 8.1(contreoxlposure) 7.2- 8.2(100mg/Lexposure) Testtemperaturreange(0- 48 hours) 19.4- 20.80C Elementbasism:ortaliatnydimmobilization -RESULTS NominalconcentrationBsk:contro1l5,,25,40,60,100mg/L Elementvalue: 24-houErC5o=> 100mg/L 48-houErC5o=44 (25- 60)mg/L StatistiEcvaalluatioTnh:e EC50valuaend95% confidenicnetervwaals calculatuesdingthebinomial/Iinnoenarinterpolamteitohnod. MortaliotfycontrolNso:ne Remarks:Valuesreporteadreforthetesstample(mixtureN)o. calculatiwoenrsemade toadjusftortheconcentratoifotnhetest substancienthetesstample. Cumulativpeercenmtortaliatnyd immobilization: Nominal Test Conc., MgA- 24-hours 48-hours Neg. Control 0 0 15 5 15 25 6 20 40 1 10 35 60 0 60 100 25 1 90 CONCLUSIONS The testsample 48-hourEC5o forDaphnia magna was determinedto be 44 mg/L witha 95% confidenceintervaolf25 to 60 mg/L. Testingwas conducted on the"as-soldm"ixtureofthesubjectmaterialin water(-25% subjectmaterial)T.he valuesreportedapplytothatmixture -andnotdirectltyothe subjectmaterial. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St.Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. This study,whilewellconducted,lacks analyticadlata for:determinationofthe testsubstance concentrationinthe testsolutionsa;nd determinatioonfthe sample purity.Because a mixture was tested,caremust be takenwhen interpretitnhge results.The solvent inthemixturecouldhave enhanced or restrictebdiologicaulptakeand/or toxicitoyfthe fluorochemicaclomponent. REFERENCES Thisstudywas conducted by EnviroSystemsDivisionResource Analysts, IncorporatedH,ampton, New Hampshire on behalfofthe3M Company. 'OTHER Last changed: 5/18/00 Study Title Static Acute Toxicity of FC-120 to the Daphnid, Daphnia magna Authors Timothy J. Ward Robert L. Boeri Study Completed February, 1992 Performing Laboratory EnviroSystems DivLsiofi Resource Analysts, Inco rporat ed one Lafayette Road Hampton, New Hampshire 03842 Page I of 14 1. SUMMARY The acute toxicity of FC-120 to the daphnid, Daphnia magna, is described in this f inal report. The test was conducted for 3H Company for 48 hours during February 5 to 7, 1992, at the EnviroSystems Division of Resource Analysts, Inc. in Hampton, New Hampshire. It was conducted by Jeanne Magazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Timothy Ward. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 IOC. The dilution water was filtered natural well water -collected at Hampton, New Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of FC-120 were: 0 mg/L (control), 15, 25, 40, 60, and 100 mg/L. Nominal concentrations were used for all calculations. Daphnids used in the test were roduced from an in-house culture and were less than 24 hours old at' the start of the test. After 48 hours of. exposure the control daphnids had an average wet weight (blotted dry) of 0.0002 g, resulting in a loading rate of 0.005 g/L. All daphnids were in good condition at the beginning of the study. Exposure of daphnids to the test substance resulted in a 48 hour EC50 of 44 mg/L FC-120, with a 95% confidence interval of 25 to 60 mg/L. Six of the surviving daphnids exposed to 60 mg/L and all of the surviving daphnids exposed to 100 mg/L were immobilized at 48 hours. No other sublethal effects were observed in any test vessel. Page 2 of 14 II. TABLE OF CONTENTS SECTION: PAGE I. Summary 2 ii. Table of Contents 3 III. Index of Tables and Figures 4 IV. Introduction 5 V. Methods and Materials 5 Vi. Results Vii. References Appendix A. Water Quality Data from Toxicity Test 12 Page 3 of 14 Ill. INDEX OF TABLES AND FIGURES Table 1. Chemical characterization of a representative Sample of natural well water used as dilution water for toxicity test Table 2. Survival and sublethal effect data from toxicity test Table 3. Median effective concentrations from toxicity test (EC50s) Table A.l. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity test PAGE 6 9 10 13 Page 4 of 14 IV. INTRODUCTION This study was sponsored by 3H Company, St. Paul, Minnesota. The objective - of the study was to letermine the acute toxicity of FC-120 to the daphnid, a freshwater invertebrate. The report contains sections that describe the methods and materials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. V. METHODS AND MATERIALS TEST SUBSTANCE: FC-120 (EnviroSystems Sample Number 5103E) was delivered to EnviroSystems on November 13, 1991. It was contained in a 250 ml plastic bottld that was labelled with the following information: "j2904-I, FC-120, Lot 164, 2-Butoxy Ethanol." FC-120 was supplied by 3M Company; 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve EnviroSystems for a minimum of 10 years. sample will be retained at DILUTION WATER: Water used for acclimation of test organisms and for all toxicity testing was collected from wells at EnviroSystems in Hampton, New Hampshire. Water was stored in 500-gallon polyethylene tanks where it was aerated. Results of chemical analysis of a representative sample of water are presented in Table 1. TEST ORGANISM: Juvenile daphnids employed as test organisms were from a single source and were identified 'using an appropriate taxonomic key. Daphnide used in the test were produced from an in-house culture and were less than 24 hours old at the start of the test. the conclusion of the toxicity test. Control daphnids were weighed at Prior to testing, daphnids were maintained in 100% dilution water under static conditions in 4 liter glass jars. During acclimation daphnids were not treated for disease and they were free of apparent sickness, injuries, and abnormalities at the beginning of the test. During the acclimation period 24 hours prior to the test initiation the temperature was 20.3*C. Daphnids were fed yeast, trout chow, and/or the freshwater or twice daily before the test. alga Selenastrum capricornutuin once Page 5 of 14 Table 1. Chemical characterization of a representative sample of natural well water used as dilution water for toxicity test Parameter pH Conductivity Hardness Organochlorine pesticides Organophosphorus pesticides ---- Polychlorinated biphenyle Unit of Heasurement Reporting Limit pH units umhoo/cm mg/L as CACO 3 -- ug/L 0.5 ug/L 2 ug/L 1 measured Value 7.2 540 166 ND ND ND Notes: 1. ND- not detected at or above the reporting limit. 2. pH, hardness, and conductivity were measured in dilution water collected from the control test vessel prior to introduction of organisms and pesticide and PCB data is collected during routine biannual water quality testing. Page 6 of 14 TOXICITY TESTING: A screening test with the test substance was conducted during January 21 to 23, 1992. Nominal concentrations of test substance were 1, 10, 100, and 1,000 mg/L. After 48 hours of exposure there was 0% survival at 100 and 1,000 mg/L and 80% survival or greater at all lower tested concentrations. A definitive toxicity test was attempted from January 29 to 31, 1992. Nominal concentrations of test substance were 150, 250, 400, 600, and 1,000 mg/L. This test was repeated because less than 50% survival occurred at all tested concentrations. The definitive toxicity test was performed during February 5 to 7, 1992. The test was conducted at a target temperature of 20 t IOC with five concentrations of test substance and a dilution water control. A 1,000 mg/L stock solution was made by combining 0.2S g of the test substance and dilution water in a 250 ml volumetric flask and adjusting the final volume to 250 mls with dilution water. Nominal concentrations of the test material were: 0 mg/L (control), 15, 25, 40, 60, and 100 mg/L. Twenty daphnids were equally distributed among four replicates of each treatment. The test was performed in 250 ml glass beakers that contained 200 ml of test solution (water depth was approximately 6 cm). Test vessels were randomly arranged in an incubator during the 48 hour test (a randim numbers table was used to select the location of each vessel). A 16 hour light and 8 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of 20 uEs-im-2. Aeration was not required to maintain dissolved oxygen concentrations above acceptable levels. Daphnids were not fed during the test. The number of surviving organisms and the occurrence of imobilization (inability to swim within 15 seconds after gentle agitation) or other sublethal effects (loss of equilibrium, erratic swimming, loss of reflex, excitability, discoloration, or change in behavior) were determined visually,and recorded initially and after 24 and 48 hours. Dissolved oxygen (YSI Model 57 meter), pH (Beckman model pHI 12 meter), conductivity (Cole Parmer number 1481-60), and temperature (ASTH mercury thermometer) were measured and recorded daily in each test chamber that contained live animals. STATISTICAL METHODS: Results of the toxicity test were interpreted by standard statistical techniques (Stephan, 1983). The 24 and 48 hour EC50 values were calculated by the binomial, moving average, or probit method. All calculations were performed by the author using nominal concentrations of the test substance. Page 7 of 14 VI. RESULTS All test vessels containing FC-120 were clear and remained clear throughout the test. Biological and water quality data generated by the acute toxicity test are presented in Table 2 and Appendix A, respectively. One hundred percent survival occurred in the control exposure. Control daphnids had an average wet weight (blotted dry) of 0.0002 g at the end of the test. Loading rate durLDg the toxicity test was approximately 0.005 g/L. Exposure of daphnids to the test substance resulted in a 48 hour EC50 of 44 mg/L FC-120, with a 95% confidence interval of 25 to 60 mg/L. Six of the surviving daphnids exposed to 60 mg/L and all of the surviving daphnids exposed to 200 mg/L were innobilized at 48 hours. No other sublethal effects were observed in any test vessel during the test. Page 8 of 14 Table 2. Survival data from toxicity test Nominal Concentration (mg/L) rep. Number Alive ---- 0 hr 24 hr 48 hr Number Affected Ohr 24hr 48hr 0 (control) 1 2 3 4 is 1 2 3 4 25 1 2 3 4 40 1 2 3 4 60 1 2 3 4 100 1 2 3 4 5 5 s 5 5 s 5 5 s 5 5 5 5 5 5 5 5 4 5 4 3 5 5 5 s 5 3 5 s 5 5 4 3 5 5 5 5 4 4 5 4 3 5 5 2 s s 4 5 5 2 5 s 1 5 s 3 5 5 2 5 3 0 5 4 0 5 3 1 5 5 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 2 0 0 2 0 0 0 0 0 0 0 0 1 0 0 1 Note: Affected organism were I biiized (unable to swim within 15 seconds after gentle agitation). 9 of 14 Table 3. Median effective concentrations (ECSOO) from toxicity test Exposure period EC50 24 hours 48 hours >100 mg/L 44 mg/L 95 percent confidence limits 25 60 mg/L Calculation method Binomial/Nonlinear Interpolation Page 10 of 14 VII. RLPP'V@RENCES OECD. 1984-., Guidelines for Testing of Chemicals. Section 2: Effects on Biotic systems. Method 202, Daphnid op., Acute Immobilization Test and Reproduction Test. Adopted April 4, 1984. Stephan, C.E. 1983. Computer Program for the Calculation U.S. EPA. Duluth, MN. Personal Communication. of LCSO Values. Page 11 of 14 -M ---1 0- Appendix A. WATER OUALITY DATA FROH ToxrciTy TEST 4 4 Page 12 of 14 .--. .-- @. ly -@@ Table A.l. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity test Nominal concentration (mg/L) Rep. Conductivity (umho/cm) 0 24 48 hr hr hr 0 (control) 1 540 580 600 2 550 580 590 3 570 580 590 4 570 580 580 is 1 580 580 590 2 580 570 580 3 580 570 580 4 580 S70 590 25 1 580 580 590 2 580 580 580 3 580 580 580 4 580 580 580 40 1 580 580 590 2 580 580 580 3 580 580 580 4 580 580 590 60 1 580 580 590 2 580 580 590 3 580 570 580 4 580 580 4 590 100 1 580 590 590 2 580 580 580 3 580 580 580 4 580 580 590 0 hr 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 7.2 pH 24 hr 8.0 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.2 8.2 8.2 8.2 8.2 8.2 r-.2 9.2 8.2 8.2 8.2 8.2 48 hr 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 Page 13 of 14 Table A.l. Continued. Nominal concentration (mg/L) Rep. Temperature (OC) 0 24 48 hr hr hr 0 (control) 1 2 3 4 15 1 2 3 4 25 1 2 3 4 40 1 2 3 4 60 1 2 3 4 100 1 2 3 4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.5 19.4 19.4 19.6 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 20.5 20.3 20.3 20.2 20.4 20.3 20.3 20.4 20.3 20.3 20.2 20.3 20.3 20.3 20.2 20.4 20.3 20.3 20.3 20.2 20.4 20.3 20.3 20.3 20.6 20.6 20.4 20.3 20.3 20.3 20.4 20.4 20.8 20.8 20.6 20.4 20.4 20.4 20.4 20.4 20.8 20.Si 20.a,' 20.7 20.4 20.4 20.4 20.4 Dissolved oxygen (mg/L) 0 24 48 hr hr hr 8.7 8.9 9.3 8.8 9.0 9.2 8.8 9.1 9.3 8.8 9.0 9.2 8.9 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.1 9.0 9.0 9.1 8.9 9.0 9.1 8.9 9.0 9.1 9.0 8.9 9.1 8.9 9.0 9.1 8.9 8.9 9.1 8.9 8.8 9.1 8.9 8.8 9.1 8.9 9.0 9.1 9.0 8.8 9.1 9.0 8.9 9.1 8.8 8.8 9.1 9.0 9.0 9.1 9.0 9.0 9.1 9.0 8.8 9.1 8.9 8.8 9.1 Page 14 of 14