Document g2r6vwgp7J2kExLJwedRx5ZbJ

BIODEGRADATION (BOD/COD) TEST SUBSTANCE ___________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of L-4647. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The test sample is 1.356% solution of L-4647. Current information indicates it is a mixture of 6.6% PFOS, 20% polyethylene glycol, 20% hexahydric alcohol, 39% sodium decyl sulfate, 13.2% Hydroxy foamer, 1% sodium ethylhexyl sulfate, 0.1% methylhydroquinone, and 0.1% biphenylol, sodium salt, tetrahydrate. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the biodegradability o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: BOD protocol 3/10/80. Type: Aerobic GLP: No Year completed: 1980 Contact time: 20 days Inoculum: Chemolite sewage. RESULTS ________ _________________________________________ Chemical Oxygen Demand (COD): Biochemical Oxygen Demand (BOD): 5-Day: 10-Day: 20-Day: 17,000mg/L 16,000 mg/L (duplicate) 7 ,7 0 0 mg/L 8 ,6 0 0 mg/L 11,000mg/L C O N C L U S IO N S ___________________________________________________ No conclusions given. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________ ;____________________________ 003024 Reliability: Klimisch ranking 3. Study lacks description and records of the method followed. The inoculum was not properly characterized. There is no characterization of the test substance purity, and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 5623S, 1980. O T H E R __________________________________________________________ Last changed: 6/26/00 003025 Form 10620-5-PWO cc: LAB REQ UEST BOOK - 42-5W PRODUCT ECOLOGICAL ASSESSMENT m Actioiraoc lvirqnrnentiri Laboratory 3D Work Sheet - Samples BEST COPY AVAILABLE -- Lab R eq. No, ................................ ...................... ........... 5 __________________ BOD Run N o .f O at* Startad < f/2 S /V - f CJ - Day BOD Readings '-- Data .tfz 3 o r S C <mnu* Lafcortitory * o tk S h M t - S a m p l BEST COPY AVAILABLE 7 .manual Laboratory t *-' norK antot oaropioe b e s t c o p y a v a il a b l e Lab B iq . N o . S V 2J < * y. OataStartad / n sm BOO Run N o .| ^ - f (J Day BOO Reading Date S - / S - 8 X1 , M tW odutioft > In M W tw i& ki * > (I --- ' r % n 7270 to ivo "*1 //OO /<& L?0 *2/00&\ V 1 t-V W ^ o f Sample diluted to / O O ml with 01 water I k 7 <? (Dilution factor - 1 1f undiluted! DuOalPlI eSrsiii 3.60 /.*,o ToajM. SPSS s.v o 7 ,6 0 TeBM|lle> BOOOay ffffC Awi|i 00 Daw * 1. Cone.,- Concentration o f ample (m l/l oi(m gflpln BOO bottle. 2. Sample Sir ml or f o f ample or dllutod ample added to BOO Bottlo. Comment hjjAiAA, ( 5 IOC* IC LX Po.mterse rwo i V . . > J-- 0 0 3 0 2 9 /a S:-; , ?6mgSj& U&esm&y '#0mflhapt*lMla, rob,ndfondM BEST COPY AVAILABLE 4 M IM % Kn V ) Request No. t 1i Typ# Soarem BOO Run No. % O at* Started ^ M ad Concentration ^ < h m /j/.s /-_______ lonseedad Dilution - Water* - Day 80D Readings - . y /s o /k j B o ttle No. In itia l O lO . A ,) . .m A jr , f & 5 Seeded (M ution Water Blank (optional) N a n tD A . (A a l f.a s a 1. S " g oo D .O .O p M on (A j * A j) .( S ' o: 0 . 50 Average 0 .0 . D e ple tion ( i-* a l 0 ,5 3 Bottle No. 3 InWfaclDt)X. Pree(eCntj)D.O. 9 ,o o sr.VJr r .z S 9 .a & _____ 2>'5s D.O(C.D-|e-Cple2t)ion o sx 0 .5 0 0 .5 0 . ... ADv(eCer^apgl-eeCtioD2n).O. 0 .5 8 .Seed Controls Bottle No. f' Initial D.O. IB, Preeent D.O. (Bal 0.0. Depletion (Bi -B2) 7?9~ A /43 _ _____.--(j2,, _____ ,2 0 y sr -5 0 % 30 O 2/ > 1 ___________________________ 7 .9 0 7 . 2J7 9 .7 5 7, 3 5 7 ,6 0 S .G Z 0 ,7 0 0 .9 5 0 .9 0 /. 6 0 i Glucose Glutamic dd Standard Bottle No. SampleSite InitMDX). Preeent D.O. (D2) /< r / <?0 f,9 5 \ . 5: 2 0 7f 7A . ? -9 0 3 ,iO Potassium Hydrogen PhtHeiate Standard (optional) Bottle No. SampleSite ' Initial D.O. * ID,) Preeent D.O. ID3) Total D.O. DIDep, le-t0io3n) 2.ZS 3. 15 5 50 Total D.O. Depletion (0, 02l Depletion Due To Standard A 71 9.. - / f.9 7 . S .^ SOD Average 3 Dev BOO /7/ /9 3 5^ T-jfiG - 2 / 3 -> Depletion Due ToStandard * Day BOD Average ~ Oey BOD fcj Foim 19795 PWO 003030 .teJLstoretory .S h e e t B lanks, and Standi* Bottla No. MW AA (At) Praaant 0.0. (A,) m j7 9 0 T sa _____ / > 5 & 50 cw tt. l aadad M u tto n -Wafer Hank (cpdansl) % 9SS -o o ~ n7W OX). Captation Al Ajl 0 .7 5 0 .5 0 0 .6 0 Avaraga 0.0. Oaplation (Si jl 0 .(^ Bottle No. XJ 3- ____i. 2.1...____ U M S.O . *1 Praaant 0X>. ,1 9 no u. 1/y X .? 5 iT v o 9 . r t O _______ 7 Z J 5 DO. Pplstlss 1 Cjl 0 ,6 0 . CW S O '. 6 ? __________ AvaragaOX). Oaplation *1 e2) 0 .5 7 Scad ControU ' BottleNo. A-/. ; <7 3______ k ____ _ InMal 0.0. (B,l ZQ <10 0 ! 2 J D _______ S' 2 .3 0 7. 9 0 7 2S' PraaantOX). a* 7 .7 5 < .2 0 05" 7 . .7 e r OA.OaMaa 19> /. 0 0 / .s o / .z z 2 .1 0 Glucose - Glutamic Acid Standard Bottla No. ------- ppo-- / SampfcSim 3 ,0 5 ,0 ,MtMD.O. tOgl 9 .5 Praaant 0.0. (0,1 125 4 .4 5 '7 -- 7 . 0 ? Q _ .4 0 - Potassium Hydrogen Ptithalati Standard (optional) l BottlaNo. SamptaSlaa InMal 04). 10,1 Plaint D.O. (0,1 Total 0.0. Oaplation (Os 0,1 2 Co5 50 .5 0 Total 0.0. 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