Document g2kday5qbdr48nGyEakxw5OkG

M &Z6 - ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE___________________________________________ Identity: A mixture containing perfluorcoctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 628. The test sample is FC-600 (US) (cc8212-19). Current information indicates it is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. METHOD:___________________________________________________ Method: Not noted. Test type: Acute static (Range-finding) GLP: No Year Completed: 1983 Species: Pimephales promelas Supplier: Commercial supplier in Brady, Nebraska Analytical monitoring: Temperature, pH, DO Exposure period: 96-hours Statistical methods: Not applicable. No mortality at highest concentration tested. Test fish age: Juvenile Average Length and weight: Length = 3.6 cm Weight = 0.39 grams Loading: 0.65 g/L Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water, St. Paul, MN. Dilution water chemistry: pH: 8.2 Dissolved Oxygen: 9.6 mg/L Temperature range: 18C Lighting: Not noted 002024 Stock and test solution preparation: Test solutions were created by direct weights additions. Concentrations dosing rate: Once Stability of the test chemical solution: Not noted Exposure vessels: Wide-mouth one gallon glass jars containing 3 liters of test solution. Number of replicates: One Number of fish per replicate: 5 Number of concentrations: Four plus two blank controls Water chemistry during the study: pH range (0 - 96 hours): 8.0 - 8.2 (control exposures) 7.7 - 8.2 (1,000 mg/L exposure) Dissolved Oxygen range (0 - 96 hours): 5.8 - 9.6 mg/L (control exposures) _ 0.9 - 9.6 mg/L (1,000 mg/L exposure) Temperature range (0 - 96 hours): 18 - 21C RESULTS____________________________________________________ Nominal concentrations: 2 blank controls, 1, 10, 100, and 1,000 mg/L Element value: 96-hour LC50 = >1,000 mg/L Element value based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The FC-600 96-hour LC5o for Pimephales promelas was determined to be >1,000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking 2. The study meets all the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solutions. 002025 REFERENCES This study was conducted by 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 8875, 1983. OTHER_____________________________________________________ Last changed: 6/28/00 002026 best copy available Type Test ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET EXPLORATORY (RANGE-FINDING TEST) Material Testedj __ LR r & A ^ Test Organism^g^^//(//iftSource Date Rec^./^y^Sj&Batch N o . / ^ 5 Av8 ` Wt* grams Avg. Sizg ^ ^ C M No. Organisms per dose 5 Test Solution Volume 3 Ltters/All-Glass Tank Tank Dimensions:WIDE-MOUTH 1 GALLON GLASS JAR Time Started Date Started ^ J p > ^ 9 ^ Date Completed _ Dilution Water Carbon-filtered well water (Temp. / f t C.. D.O. ^///? ppm, pH f t . ? \ Investigator J W ) / (Full Signattuurre)) /7 LC5 0 Confidwlie Limits) J^lIOQQ Wg/f Init. ik Cone. >* sTotal -------------2^ 1 Hour Amount Total D.O. Added No. Dead pH ppm 46 fours -- 73m ours '""96 Hounrs Total D.O. Total D.O. Total D.O. No.Dead pH ppm No.Dead ppm No.Dead >H ppm r Control L H1 5 ... \oz2. _ - S O rS m o ., 5 0 Q . In n o w fU //fi fif, W-, FX if4 _____ fiO w 3 ,1 X)... f i r l?<fa 0 <0 Si. (04 n 0 (r,<S 0 0 . Si0 *}<i 0 -. -Ko 0 ... 1if) OR 0 v; R f faj (fi *<4 i"*htT hl *4 h(O 0 1if) /r, ft fa? s i OiP> 1 RP) ffr3 , 1 Test Te*>. C. /? ' I n t M a U t Date c o m e t s :* i^ m u q x jfA g ... a <?\ Z fifr 'Ox's ^//A Effects other than Mortality ^50 Tank No. Symptoms or Reactions Control T rr 0=^-1 ftr D - 3M Comments ' 24-Hr. A /fa /J tr. A/uM eC.S U uJxr A /ja J C Total No. Affected 48 Hr. /A ^ jo? 72-Hr. /A //A rl M mz _ A Joi/ ' ^ /M .' . 1 >. . 96-Hr. :___ --------- Um k //( ? ?' -- Q Q j2 0 **:^