Document g2YJbrm0oQbVV5Enyvv46Drda
MICROBICS MICROTOXC TOXICITY TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, oras a component of FC-203CE. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 519. The testsample isFC203CE. Currentinformationindicatesitisa mixtureof 1.84% PFOS, 30% diethyleneglycolbutylether,60.23% water,2.56% Sultone foamer, 3.1% sodium octylsulfate2,.1% polyoxyethylenemonooctylphenyl ether,0.12% sodium laurylsulfatea,nd 0.05% tolyltriazole.
The followingsummary applies to a mixture with incompletely characteilzed concentrations of impurities.Data may not accurately retlectthe toxicityof the tluorochemical component of the testsample.
METHOD:
Method: MicrobicsMicrotoxS Procedure Test type: Acute static GLP: No Year Completed: 1989 Species: Photobacteriumphosphoreum Analyticalmonitoring: pH and osmoticcondition. Exposure period: 30-minutes Statisticamlethods: EC50 valuescalculatebdy statisticlailnearegression program providedforMicrotoxV Test organism source: MicrobicsR'eagent. Test conditions:
Diluent: Microbics2% NaCi solution Test temperature: Not noted. Stock and testsolutionpreparation:A primary2000 mg/L stocksolution was prepared inMilliporMeilli-QTw"ater. The pH ofthe stocksolutionwas 6.8 and an osmoticadjustmentwas made using 200 mg NaCl dissolvedin10 mL stocksolution.Appearance oftestsolutionswas noted as "completely miscible,no colorapparent".Alltestsolutionswere made by proportional dilutions. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blank control Element basis: Percentlightloss
RESULTS
Nominal concentrations: Blank control,125, 250, 500, and 1000 mg/L
Elementvalue:
5-minutEeC5o= 370 (360- 380)mg/L 15-minuteEC5o = 260 (250 - 270) mg/L 30-minuteECro = 230 (220- 240) mg/L
Element values based on nominal concentrations.
Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsubstance 30-minute EC50 forPhotobacterium phosphoreum was determined to be 230 nig/Lwitha 95% confidenceintervaolf220 to 240 mg/L.
Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
Reliability:Klimischranking2. Testingmeets allcriterifaorqualitytesting. However, sample puritywas not properlycharacterizedand the study lacks analyticaclonfirmationofthe amount offluorochemicalproportioninthe solution.
REFERENCES
This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number G 1358-1,1989.
OTHER
Last changed: 6/26/00.
ENVIRONMENTAL LABORATORY MICROBICS MICROTOX R SYSTEM
RAW DATA
LR
06
TEST SUBSTANCE: TEST DATE ANALYST:
A 03 C E- I A+ S-lq
SAMPLE CUVETTE
FINAL ASSAY CONCENTRATION no ig
-INITIAL---
FINAL READTMS
READING 5-MIN. 15-14IN
1(0)
1(5) 1(15)
30-MIN. 1(30)
Bl
BLANK CONTROL
q c 7y
70
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DUPLICATE
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LR 61359-1
FC-203CE
-MICROTOX(r) DATA SHEET 5-MIN EXFIOSURE 6/16/89 SAS
PAI*R
CONC.
1
125.000
2
125.000
3
250.000
4
250.000
5
51)0.000
6
500.000
7
%1000.000
a
%1000.000.
BLANK Bo/Bt= 95 BLANK RATIO= 0.8526
@Io/It
88.0/ 55.0 96.0/ 59.0 92.()/46.0 97.0/ 49.0 87.(.)/:7.2.0 93.0/ 34.0
94.0/ 24.0 96.0/. 23.0
81
B-OBS
0.364 0.387 0.7o5 0.688 1.318 1.332
2.339 2.559
S-EST
0,375 0.375 0.702 0.702 1.313 1.313
2.456 2.456
EC 50
37o.OC)l
R =0.99915
SLOPE
:358.3E)7 TO 381.992 1. IC)45 INTERCEPT
370 (3tiop-.3SON@
+5.9135
RES. 1 0.054 2 0.052 3 0.035 4 0.035 5 o.035
6 Ci.035 7 0.051 .el 0. 01@J5
PROB. 98 90 40 .41 41 41 as 100
----------------v --------------1>
?*mnt &I
57 57
70 7oq .......
10 -8-1<
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2 ---------------- --------------- ----------------
4
5 LOG CONC.
6
7
@LIST
2RUN
L0AD
C 0N T 6
L F, T 1
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L.R 61358-1
FC-203CE
MICROTOX(T
DATA SHEET
15-MIN EXPOSURE
6/16/89 SAB
PAIR.#
1 2
3
4 5 6 7 a
CONC.
milk 125.000 125.000 250.000 250.000 500.000,
500-000 %1000.000 %looo.000
BLANt-l" Bo/Bta 95 BLAN@: RATID= 0.7789
IQ/It
88.0/ 45.0 96.0/ 49.0 92.0/ 37.0 97.0/ 39.0 87.0/ 25.0 93.0/ 26. C)
94.0/ 18.0 96.0/ 17.0
74
B-OBS
0.523 0.526 0.937 0.937 1.711 1.786
3.068 3.399
G-IEST
o.sig
0.519 0.953 0.953 1.749 1.749
3.210 10
?*Merv+
3 Lt 49 ..42
3
75"
77
EC 50
264.385
254.982 TO 274.134
2.50 7o
R =C).99904 SLOPE
1.1388
INTERCEPT
+5.5774
RES.
PROB.
---------------y---------------y--------
1 ().C)56
91
2 0.056
91'.
7 O.o37
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4
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LR B1358-1
FC-203CE
MICROTOX(R) DATA SHEET
30-MIN EXPOSURE
6/16/89 SAB
PAIR@#
2 4 b
CONC.
Y-45i
125.000 125.00o 250.1)oo 2!50.(@)0() 50(). 5()0.00() % 1000. (")(,)0 % I C)OO (:11,)1)
BLA NK' Bo/Bt= 95 BL.ANK RATIO= 0.7368
ID/It
88.0/ 41.0 96.0/ 45.0 92.0/ -)3.() 97.0/ -@5, 8*01.0/ '-I, . 93. (:)/'-2.
94.01 1 Fi.0 ()6.1)/'14.
70
(B-OBS
0. 5E32 0.572 1.054, 1. C)42 2.U53 2.115
ZS.618 4.053,
G-EST
0.570 0.570 1.078 1.078 2.o37 2.0--7
3.850
PerceilL'l+ot 37
iv to 7
E(','JO
230. 637
R -C).99876 SLOPE
220.720 TO 241.(.)Ol
1.0861
INTERCEPT
30 ;to
+5.4408
tA,%
RES.
F'ROB.
1 0.063
91
2 0.064
9:@
0 . 0 4."-z
41
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42
t@ 0.042
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42
0. @.)62
86
2 ----------------V ---------------V ----------------
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li-IST 2RUN
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C0NT 6
FF
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y
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---------------- ------------------------------
4
5 LOG CONC.
6
7
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