Document g0wdGZmdKQ9Z6ZpgNzYyX0j3

ACUTE TOXICITY TO FISH (BLUEGILL) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium saltC,AS # 2795-39-3) Remarks: The 3M productionlotnurt@ibwears notnoted. The test sample isFC-600. Currentinformatioinndicatesitisa mixtureof 1.0% PFOS, 81.20% water,12.00% diethyleneglycolbutylether,1.00% sodium octylsulfate2,.00% propane Sultonefoamer, 1.00% sodium decylsulfate, 0.85% xanthangum, 0.1% N-(3-chloroallhyelx)aminium chloride0,.80% starch,and 0.05 % benzotriazole. The following summary applies toa mixture with incompletely characterized concentration of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the test sample. METHOD: Method: Not noted. Test type: Staticacute GLP: No Year Completed: 1975 Species:Lepomis macrochirus Supplier:Not noted. Analyticalmonitoring:Temperature,pH, DO Exposure period:96-hours Statisticamlethods: Probitanalysis. Test fishage: Juveniles Average Length and weight: Length= 1 inch Weight = 0.5grams Loading: Not noted. Pretreatment:Not noted. Test conditions: Dilutionwater: Carbon-filterewdater,CityofSt.Paul,MN Dilutionwater chemistry: Not noted. Lighting:Not noted Stock and testsolutionpreparation: Test solutionswere createdby directweights additions. Concentrations dosing rate:Once Stabilityof the testchemical solution:Not noted Exposure vessels: Not noted. Number of replicates:One Number of fishper replicate:20 Number ofconcentrations5: plusa bl,3nckontrol Water chemistryduringthe study: pH range (0- 96 hours): 7.1- 7.2(controelxposures) 7.2- 7.3 (2,000mg/L exposure)* Dissolved Oxygen range (0- 96 hours): 5.6- 7.0 mg/L (controelxposures) 4.5- 6.0 mg/L (2,000nig/Lexposure)* Temperature (0- 96 hours): 720F Values forthe 2,000 mg/L exposure (thirdhighestconcentration) were used because totalmortalitoyccurred inthe highest concentrationstested. RESULTS Nominal concentrations: Blank control,1,000, 1,500,2,000, 3,000, and 4,000 mg/L Element value: 96-hour LC50 = 1,500 (1,282- 1,755) mg/L Element value based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas described inthe Test Substance Remarks field.The value reportedappliesto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-600 96-hour LC5c)forLepomis macrochirus was determined to be 1,500 mg/L witha 95% confidenceintervalof 1,282 to 1,755 mg/L. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking3. The study lacks documentation on methodology. The sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmationof the amount of fluorochemical proportioninthe solutions. REFERENCES This studywas conducted by 3M Company, Environmental Laboratory,St. Paul, MN, 1975. OTHER Last changed: 6/28/00 lp 740101 EWIRONMEMAL ENGINEERING LABORATORY AIQUATIC TOXICITY WMX MUT Type Test 96- HoUr s-LatieMat*rial Test*d--Fc -go6 Test Organian Date Started 9--71- AvS. Voight Date CoWl*tod ASvig. 9* TW, (7 - Tim Started Dilution Water ea@nh :ad jqm Pitte - Platted LCS- I@v I - I-- - ,(P-Ct>PL/S Conc. 24 hrs % D.0 Survival ph__pps Control /0 48 hrs % D.0 Survival P-H pm 100 '7.2 72 hrs % D.0 --Survival9H gm 100 -r1. 60 96 brs D.0 Survival pH ppo 00 '7/, 5.6 /oo -7z-.-.65 -/00 /00 7.3 6@ 74 AverageTen@@,,rl Coments: -/00 72 6Htt t '70. 5,91. 14o i 6.4 !@@0 -50 7,3 5,E; @4 0 4.1; 0 7:? e4,o 0 72- - ZL'l -7..l4@c- (,7 I-CSOCalculation 100 4 'T tt IT t u 10 0 10 20 30 40 so 60 70 so 90 100 Survival ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY - PROBIT ANALYSIS WORK SHEET Dtte Started: Time Stai-ted: --2 ?j0 C) Material Tested: (PM) Test Organism jFc-,6oo T)rpe Test: (Contintmas-itMw)(Static) Avg. Wt. t@, (jgram) Avg. Size Exposuie Period: ?,K-Hour - Date Completed: Diluent:(carbon-filt.-St.PC.ity Wtr. Analysis by: (1) K No. of Doses Plotted 12 3 4 Conc. jI ,Soo Observed Mortality -,o@- F-o) -7c> /00 (2) Expected Mortality 1.3 - 5o (3) Corrected Values 0E ObservedExpected -- 7 @j (inch) Contribution to chi(from Nomograph #1) 6 7 K Animals/Dose Total Animals = 2 chi (P-.05) for (K--2) -,2C) Total contribution to chi (chi2)= contrib. to chi x Tot. Animals degree of freedom (fromtable 2) 30 4.6o is less than therefore, the data are not significantly heterogenous. 95% Confidence Limits for Tr L C 64 1s-0 iz i LC so Slope function (S) -LI@-Il/- /LLCc 84 so + LCso /LC 16 02,@?71 LC 16 + t5c,,/v-sc\,Al@ +@,Ai;z 2 Total number of animals used between 16% and 84% expected effects (N')- _A 2.77/A, fLcso = (S) 1-kA m (from NomolZraph #2) LC5o/fLCso - lower limit LC so X fLC.50 = upper limit Soc, k-ki u \--)A /, --)-AL M-i-L@ Cl LC so (95% confidenc e limits to L-) 5 S-) (1)Do not list more than two consecutive 0% effects or more than two consecutive 100% effects. (2) Expected value for any dose should be greater than 0.01% or less than 99.99%. (3)Corrected value for each 0% or 100% effect (from Table 1). There is a 95% chance that the true value of LC lies within these limits. so 7i 7 1; t LQ 7@- 4.L.i@i 17 T 41 T ri 14 4 -14 J4 loft co 0on 0 0 0V) aC4 La