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ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: rPeefrefrlrueodrotoocatsanPesFuOfSfoAnAy,laFmiCd-o1(2e8thoyrl)aascetthaetem;ajmoarycaolmspoobneent of FC-127, FC-129, FC-109, or FC-109-X. (Glycine, N-ethyl-N[(heptadecafiuorooctyl)sulfonyl}-, potassium salt, CAS # 2991517) Remarks: information The test sample is FC-127 (F-6390, Lot 1). Current indicates itis a mixture of 40-44% test substance, 32% water, 14% isopropanol, 4% ethanol, and 6-10% C.-C fluoroalkyl carboxylate compounds. The following mixtureofthe stuesmtmsaurbystiasnacbebrinevaiawtateedrb/eocrgaaunsiec the test sample is solvent solution. a Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD: Methods: EPA protocol. Test type: Static acute GLP: No Year Completed: 1981 Species: Pimephales promelas Analytical monitoring: Temperature, pH, DO, and conductivity RESULTS Nominal Element vcaolnucee:ntrat9i6o-nhso:urBLkCcsont=ro8l6, 365(07,968-7805,0)11m5g0/,L.1550, 2100 mg/L Element value based on nominal concentrations. hRoeumrasrk(ssu:rfTahceerfeilwmafsormaatsliiognh)t.Tseesptairnagtiwoansinctoensdtuccotnecdenotnratthieonmsixatfuterreo2f4 the vteasltuseurbesptoarntcede aapspldieesscrtiobtehdatinmitxhteutreestansdubnsottatnhceetreesmtasrukbsstfainelcde.. ThNeo Saotltveemnpttswoarswhmaatdeportotidoentoefrtmhienetotxhiceitiympcaacntboef ctohnetprribeusteendcteootfhtehe organic Perfluorooctanesulfonylamido(ethyljacetate. CONCLUSIONS 000225 The 863 FC-127 96-hour mg/L with a 85% LcConsfifodrenPciemeinpthearlvaelsopfr7o9m6e-l8a5s0wmags/Ldebtyermmoivniendgto be average angle method. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY tReesltiianbgi,libtuyt: lKaickismiasncahlyrtainckailncgon2f.irTmeasttiionngomfeteetstssalulbcsrtiatenrciaefcoorncqueanltirtaytions. There is a lack of characterization of the test sample. REFERENCES This study Paul, MN, LwaabsRceoqnudeuscttenduamtb3erM7C0o0m9p.any, Environmental Laboratory, St. OTHER Last changed: 5/18/00 000226 . BESTCOPY AVAILABLE +, lS yw ENVIRONMENTAL LABORATORY ta07 Type TostK lostV , UT SATE OMar- orint TestedS227 F=(p390 Lov Test OrganisabATHEAD MIMNOWSource.KURIZS,BugsyRec'd [28/8 paten No SF ato we _OLZ0S prem Ave. S158B.000 ho. Orpsnisms par dose _s Test SolutionVolume3Liters/All-Gless Tank Tank Dimsmsions:WIDE-MOUTH | GALLON GLASS J Time StartedJ0IQ0AM Date sured_Z/20Q/8/ Datecomivees Z24/B1 Dilution Water_Carbem-filteredwellwater (Temp. 12 -- 0.0. 10,0 por, W&2ZJ TE TE er ee ET, mime (Pull Signati ZY ogy (95% Conflomes umes__________ lem [|= 0 Rilzsll [Bll bdo] 0 kia Clam l----1 RAzdl 0R2LAI ORACHA O 4, Lio =o Oo BAZAN OQ RZ CO kiL NC346 (Zon =nll 0 gard 0 RZWAl 0 RAZA O A235 [3Tsao lisooll o Razell o RAZZ 0 RdLIl 44 [# Tono (=0ooll-0 BARA Va RAS 275 0.51 2514) La1zooo enn ll 248 RsIgA] 3/5REVAL XE.3 5/5 40 Cr rT vr Ltncoe 1S221K 0s722068052a=B17S 22 coments: * INDIANALOFAGHTS. TEST WATER 1S ACLEPR, ASMREER Ahb AGUID&, AERAS RDR ISPR ENSED IN (HATEEREA, T AMBIENSTUREE00Fu E f ES hm CT IRC |Ppa LC | 7TI | | 5 | gL 3 A n1 n 43 un 1 nr ---- 1n -- u 1 susnrsuraadl 1 WS 1 lsualr la s Nm Za Hc AnI e2 ants (0 Ner CE IWCe RRESA E IMR 0; CoNEACTATION RETWEEN Zu Aww Tove. ee eseee SN LKONMENTAL LABORATORY JESTCOPY AVAILABLE >5Fivimrve (Fiti-scate seen 1x 7092 ype testuQroE,SSfAeariuateriar restFed-f1p73Qtot | Tet ComnianM EATUEAD U KueTzG Eluegsaafece Recta28/m5 aven +Sg Avg.Wt.0,25| grams Avg. Size, KO om Mo. Organisms per dose Z0)loading Factor_(J. 5 a1 Fish Disease Treatment: AS td YZ7 SCLAEC 20 /8/a/ Test SolutionVolume/(/7Liters/All-Glass Tark Tank Dimensions: 35x20 cm and 25 cn deep Time Started_J):SY)Hfvatestarts DateCompleted 7451/8] Dilution Water Carbon-filtered 5 (Temp. Cy B.2mw B.S a-- AY ic, on ccs3 mpl C I E ra EolleeA Pe ePT ~~ en urs 48 fours 2 far 56 fours Blanlmad | kasd= Ba 5d kbo d7d Bdo AR ssplaiond = BAS 5 lhol[I BAY Bd Blaokacd Biliol1 Ran Z0 lod20H] I 11 11T 111 11 -- lel Le 1 20|7 Zo.| 27 foit :Tiatae--lTs=S% 27 SI2 RT=o8 CBI TES 702 Tt20500 TM 4 DUAL IEG TayWATER) TofC Ca re LOS I FRc . QALEX, AT AISET Seow TEWPFRAIUET SAT Sperry FTER IH econdary H FE Ehn n Mortality) ng . LS Symptomsor Reactions mealy TERS ood BR ISudetwe [Rae TRNr TA |4p L| Blourenowg TAA TA. TA|Ad| Blowehomg [Ana TA TAS TTA RES [AT meLI AA N ImmG ---- . LV LKUNMENTAL LASORATORY BESTCOPY AVAILABLE oirininve(FUii-S8AEa"Tesh 1a os _ ve rA eo loR E mteriat restosFTol2 Ea39000t/ re Oxon MU FouroEeluazgene rec'al5)fjm2ae8ch 1_S-8)/ wg.we25)|gr, ams Avg. Sa Organisms per' doseZ()loading Factor_(3), - "ish Disease Treatment: -- = [SE rbpra Pape re TR rest Solution Volume JlrsSoiG1ans Tark Tank Dimensions: 35x20 om and 25 cm deep tinestarted J) 1AA)fMfvate starts 127/61 DateCompleted ZL5/8/ SAlution Water Carbon-filtered per (tere. 20) *c., 0.0.89pm ow 8,5 tvestigator_ om 77H)Td ie 0 Ho=arce Limits) (Fall Signature) MBNEQ BR oo Jmicior] rotar SR Four_sTRORIT ANA48L ouYrSs Tos 56 Tours lone --10 lod7A | aloe]V Rilbozl| Edis 1 TTI 111 111 10 nie ES OL PRE i em tia Tatl s--sFSF 77 BO pestras2ELLE Zo Z2r er a anus) (UFO TESTS MAT, ERA a AL LEAR. 28 ee hau. EAST DEEENMSED IN LATTES St AMBIENT ROOM TEMPT<R3ReAT<UfoR dpdEld ee ETTT boafr enemy OSEA TAN Ta Th 1Ae c.e| INWTE A A TAOT NT G