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Study Sponsor: Issue Date: Document Reference No.: ATOCHEM 4-Jun-92 AR226-2972 Acute Toxicity of Daphnia Magna Author(s): J.C. Bautonnet A.M. Petit English Translation of Report Summary for DuPont Chemical Solutions Enterprise 27-Jan-OS Company Sanitized. Does no! contain TSCA CBI r ATOCHEM Levallois Applications Center Analysis - Environmental Services Eco-toxicology Laboratory TEST REPORT INHIBITION OF DAPHNIA MAGMA MOBILITY according to standard ISO 6341 Substance studied: Name: Batch reference: CAL sample reference: Person responsible for test : Person carrying out test: Date of definitive test: Date report written: J. C. BOUTONNET A. M. PETIT 1/6/92 4/6/92 Results Application of standard test ISO 6341 (French edition 1989) to the substance referenced above has given the following results : (calculation by linear regression, limit values at 95 %) The CEfl) 50 - 24 h is : CEfl) 50 > CEfn50< r = 8.94 6.57 12.17 0.987 mq/l ma/l ma/l The CEfl) 50 - 48 h is : CEfl) 50 > CE(I) 50 < r = 3.78 3.00 4.76 0.994 ma/l ma/l ma/l Additional results: Minimum concentration corresponding to 100 % immobilization in 24 h Minimum concentration corresponding to 100 % immobilization in 48 h > 75 mg/l 75 ma/l Maximum concentration corresponding to 0 % immobilization in 24 h Maximum concentration corresponding to 0 % immobilization in 48 h 0.5 me < 0.5 ma/l Company Sanitized. Does not contain TSCA CBsl Substance studied: Validity clauses: Reference substance (potassium dichromate): The CEfl) 50 - 24 h is : CE(n 50 > CEfn50< r = . 0.92 0.83 1.02 0.997 (normal values : 0.9 to 2.0 mg/l) Dissolved oxygen content in the lowest substance concentration Giving 100 % immobilization at the end of the test: 7.90 I mg/l (must be > 2 mg/l) Percent immobilization in the control containers: 10 % (must be ^10%) Preparation of mother and test solutions : Weigh : 30 mg in 100 ml of dilution medium (3 g/l) Dilution :1/30:10 mg/l Biological information: The test was carried out daphnias with an age less than 24 h (selected by sieving). The growth (culture) was effected in ASTM medium; the food was algae collected in the exponential growth phase (Selenastrum capricornutum). ma/l ma/l ma/l Other pertinent information for carrying out the test: Company Sanitized. Does not contain TSCA CBI NOTE A L'ATTENTION DE Copies : Elf Atochem SJL Centre d'AppIication de Levallois Ie, SJuin 1992 ,,,.' ?tt!SB ae^l^- W-i" .<'tii.. i'.l/""" M. CORPART Mme BAKES Labo Ecotox OBJET : Rapport d'essai -joint Ie rapport d'essai de la toxicite aigue vis a vis de la daphnie, JC. BOUTONNET Company Sanitized. Does not contain TSCA C8I ATOCHEM Centre d'Appiieation de Levaflois Service Anafyse/Environnement Laboratoire d'Ecotoxicologie RAPPORT D'ESSAI INHIBITION DE LA MOBILITE DE DAPHNIA MAGNA selon la norme (SO 6341 Substance etudiee Norn: Reference ou lot: Numero d'enregistrement CAL: Responsable de I'essai: J.C. BOUTONNET Realisation technique: A.M. PETIT Date essai definitif: Date rapport: 1 /6/S2 4/6/92 Resultats L'application de la norme ISO 6341 (edition francaise 1989) a la substance ci-dessus referencee a donne les resultats suivants: (calcul: regression lin^aire, valeurs limites a 95%) La CE(l50-24h est de: CE(I)50 > CE.(1)5Q < r = 8,94 6,57 12.17 0,987 mg/1 mg/1 mg/1 La CE(l)50-48h est de: CE(1)50 > CE(1)50 < r = 3,78 3,00 4,76 0,994 ms/l mg/1 mg/1 Resultats complementaires: Concentration mini correspondant a 100 % d'immo. en 24 h: Concentration mini correspondant a 100 % d'immo. en 48 h: Concentration maxi correspondant a 0 % d'immo. en 24 h: Concentration maxi correspondant a 0 % d'immo. en 48 h: Company Sanitized. Does not contain TSCA CBI Page 1/2 Substance etudiee Clauses de validity: Substance de reference (bichromate de potassium): La CE(l)50-24h est de: CE(1)50 > CE(1)50 < r = 0.92 0,83 1,02 0,997 mg/1 mg/1 mg/1 (vaieurs normales : 0,9 a 2,0 mg/1) Teneur en oxygene dissous dans la plus faible concentration de substance donnaht 100 % d'immobilisation en fin d'essai: 7,90] mg/1 (doit etre > 2 mg/1) Pourcentage d'immobilisation dans les recipients temoins: | 10] % (doit etre < ou = a 10 %) Preparation des solutions meres et solutions d'essai: Pesee:30mg dans 100ml de milieu de dilutions(3g/l). Dilution: 1/30 :10mg/l Informations bioloaiaue's: L'essai a ete realise sur des daphnies d'age inferieur a 24 h (selectionnees par tamisage). L'elevage est realise dans du miKeu ASTM; la nourriture y est consiituee d'algues recoltees en phase exponentielle de croissance (Selenastrum capricornutum). ___________ Autres informations relatives au deroulement de I'essai: Company Sanitized. Does not contain TSCA C5! Page 2/2 Company Sanitized. Does not co"'n Tec-a ^ra