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ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component ofFX-3103. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted.The testsample isFX-3103, 3%. Currentinformationindicatesitisa mixtureof0.96% PFOS, 20% diethyleneglycolbutylether,50.56% water,15% ethyleneglycol4,.32% sodium octylsulfate6,.0% polyoxyethylenemonooctylphenylether,2.5% propane sultonefoamer,0.5% sodium nitrate0,.06% sodium laurylsulfate, and 0.1% benzotriazole. The followingsummary applies toa mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorachemical component of the testsample. METHOD Method: OECD 209 Test type: Staticacute GLP: No Year Completed: 1985 Analyticalmonitoring: Dissolvedoxygen concentrations Statisticamlethods: Resultswere determinedby calculationfthe% inhibitoninrespiratiornate. Test organism source: Activatedsludge mixed liquorcollectedfrom the Metro Waste Treatment Plant,St.Paul,MN. Test condition: Dilutionwater: Not given. Synthetic sewage: per OECD 209 Reference and testsolutionpreparation: Not given. Test vessels: Not given. Number of concentrations: fiveplusblankcontrol Temperature: Not given. Total suspended solidsand pH on day of testing: Not given. Element Basis: Respirationinhibitioans determined by oxygen consumption. RESULTS Nominal concentrations: 2 Blank controls,100, 180, 320, 560, and 1000 mg/L Elementvalues: 30-minuteEC50=>1000mg/L 3-hourEC50 >1000 mg/L Element valuesbased on nominal concentrations. Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 3-hourEC50 foractivatedsludgerespiratioinnhibitiownas determinedto be >1000 nig/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Study lacksdescriptioannd recordsofthe method followed.The inoculumwas notproperlycharacterizedT.here isno characterizafioonfthe testsubstance puritya,nd the studylacksanalytical confirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES Thisstudy was conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Cl 111-1,1985. OTHER Last changed: 6/26/00 b;U 7@7 ill@@ r>"C/ oily q p 21J7 Ll T39n SOAT bW Q= u .1 -017 r Irz .1 !ii- .v M ICFPBW%I -Tc>ylCA-ty Ac:nVAIMbS"At)kS-+iC.SPr;gkTOM lt444%Bk-nCW-rSSrCAkZUUVCWS-OECDZCA 06/ Z, cwteet4 Com;suttqpncy-t4a@A-M (*it Wl -rE.',c''r,SAAZS-q*4M C Coqrfptam Ce CZWTfpj avd WIVL-) An TEs-r -1 c@@wr-h nic lugm 100 -Ito q Z-60 OL4q loc),w:S(@ Lvp--h m IL-I .1.0000 SA A A- U o(oc) ,t@q00 At.tzvvpzitjrTcy);-rresr 670 Cc*x@@f-. Kim