Document evnKpj7e1ryQ4vkaD9M8R2NZp

ERI Analytical______ P. O. Box 2024, Tyier, Texas 75710 2026 Republic Drive, Suite A, Tyler, Texas 75701 Ph: (903) 534-5001 Fax: (903) 534-8701 www.ericonsuiting .com Date: September 24, 2008 File No: ERI AS # 0808198B Case Name-Jobn A. Phillips #449-86-9741 Specimen: A package containing five blocks [SS08-23762 3 I, J, K; S085083, CB08207] and twenty-one slides [S08-5083 LO, 5, 8, 39,40, 51, 54, 59, 60, 61, 62, 67, 71, 72, 92, 93, 99; CB08-207, B08-207, S08-5083 1,2] was received on 8/27/08 The material was sent by: Ms. Chelsea Tran Paralegal Williams, Kherkher, Hart, Boundas, LLP 8441 Gulf Freeway, Suite 600 Houston, Texas 77017-5051 Date received: See above. Date analyzed: 09/08/08 (Light Microscopy for Ferruginous Bodies-RFD) 09/16/08 Sample B- (ATEM- J3 Resources) Morphology of Specimen: Authorization was received from Ms. Tran in a communication dated August 26, 2008 for digestion ofparaffin embedded tissue that would permit determination ofthe presence of ferruginous bodies (FB) and uncoated asbestos fibers. Approximately one half of the lung parenchymal tissue on blocks SS0823762 31, 3J, and 3K were dissected as representative samples from right lung. As much paraffin as possible was cut from the edge of the block face adjacent to the tissue after which the tissue from the representative right lung samples underwent deparaffinization. The procedure for deparaffinization was as follows: the tissue was put on a hot plate to melt some of the paraffin, after which the sample was put through 6 changes of xylenes and 6 changes of ethanol. The digest pool from the deparaffmized pieces of tissue representing the right lung (ERI A.S. No. 0808198B) contained 0.6398 gm deparaffmized wet weight of tissue. The respective samples of tissue from right lung were carried through the modified bleach digestion procedure and aliquots ofthe digestate were collected on either 0.22 pm pored mixed cellulose ester filters for analysis by light microscopy for the presence of ferruginous bodies or on 0.2 pm pored polycarbonate filters and prepared for analysis as to the presence of uncoated asbestos fibers and ferruginous bodies by ATEM. ERI Analytical Light Microscopy: For this evaluation one fourth of the mixed cellulose ester filter from the representative sample was mounted on a glass slide, cleared (made transparent) using acetone vapor and then scanned by light microscopy at 200-400x in an Olympus light microscope. Filter 0808198B dl F4 representing the digestate samples from the blocks oftissue from the right lung was scanned by light microscopy and represented by 0.19686 grams deparaffinized wet weight of tissue. The area scanned contained forty-six classical ferruginous bodies. The forty-six classical ferruginous bodies were equivalent to 934.7 ferruginous bodies/gm deparaffinized wet weight oftissue. The limit of detection was 20.3 ferruginous bodies/gm deparaffinized wet weight of tissue. ATEM Morphology: For electron microscopy analysis a strip was cut from the carbon coated polycarbonate filter representing each of the areas sampled. These strips were mounted on 100 mesh copper grids and the filter matrix dissolved by the use of chloroform. This resulted in the production of a carbon extraction replica containing the entrapped fibers and other particulates. Scans were made at 15,000x with counts and analysis including all fibers greater than or equal to 0.5 pm in length and with an aspect ratio of greater than 5:1. A scan at lower magnification (2,000x) was made of additional grid squares in an attempt to find ferruginous bodies. The cores of any ferruginous bodies found in the area scanned were analyzed as were any uncoated asbestos fibers (>3pm). Filter 0808198B dl FI represented a 0.0787gm deparaffinized wet weight aliquot of the digestate from the samples of the right lung. An area ofthe prepared grids from this sample consisting of 1.1745 mm2 was scanned at 15,000x. There were sixteen crocidolite asbestos fibers found in area scanned at 15,000x. The sixteen asbestos fibers were equivalent to 66,644.8 fibers/gm deparaffinized wet weight oftissue. There were no ferruginous bodies found in the area scanned at 15,000x. The limit of detection at 15,000x was 4165.3 fibers/gm deparaffinized wet weight of tissue. An additional scan at 2,000x was carried out on sixty grid squares on three grids for the presence of ferruginous bodies and uncoated asbestos fibers (>3pm). There was one crocidolite cored ferruginous body and four crocidolite asbestos fibers (>3pm) found in the area scanned at 2,000x. . Background 469: There were no asbestos fibers equal to or greater than 0.5pm found in a scan of 1.1745 mm2 made at 15,000x. There were no asbestos fibers or ferruginous bodies found in a scan 2.349 mm2 at 2,000x. Limit of detection: Limit of detection is defined as that concentration below which a single fiber or ferruginous body would not likely be detected. Light microscopy analysis for the presence of ferruginous bodies performed by Ronald F. Dodson, Ph.D. Analytical Transmission Electron Microscopy performed by J3 Resources Final Report Approved: ERI Analytical Tracy Foster, B.S. Director: Analytical Laboratory -ERI Consulting Dodson Environmental Consulting, Inc. Senior Consultant ERI Consulting, Inc.