Document evJgRyRr6xDdz74n4nZKqbmLE
ARR26 - 0770
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
IESTSUBSTANCE~~~
Identity: Mixture containing perfiuorooctanesulfonate which may also be OrecftearnreesdutlofoansicPFacOiSd,) (FCC-A9S5#, 2o7r9a5s-3a9-c3o)m.ponent of FC-203FC. (1-
Remarks: The 3M production lot number was not noted. The test sample
is FC-203FC. Current information indicates it is a mixtureof 0.85% PFOS,
17% diethylene glycol butyl ether, 74.46% water, 2.34% acrylic foamer, 1.7%
alkyl ether sulfate, sodium molybdate,
20..5855%%stordiietuhmanoocltyalmisnuel,faaten,d00..1024%%stoodlyilutrmianziotlrei.te,
0.09%
The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical componentofthe test sample.
METHOD
eee ere errr
Method: OECD Guideline 209
Test type: Static acute
GLP: No
Year Completed: 1997
Analytical monitoring: Dissolved oxygen concentrations, temperature, and
PH.
Statistical methods: Results were determined by calculation of the %
inhibition in respiration rate.
Test organism source: Activated sludge mixed liquor collected from the
Metro Wastewater Treatment Plant, St. Paul, MN.
Test condition:
Dilution water: Carbon-fitered well water (3M well #2, St. Paul, MN)
Synthetic sewage: per OECD method
rReeffeerreenncceesuabnsdtatnecset,s3o,l5u-tdiiocnhlporreoppahrenaotli,onw:asApsrteopcakrseodlubtyiodnisosfotlhveing
500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM
water, then brought to the point of incipient precipitation with 1N HSO4
and diluted to 1Lwith Mill-QTM water. The pH of the reference solution
was measured to be 7.7. Test substance was addeddirectlyto the
test vessels.
NTeusmtbveerssoeflsc:oncNeonttgriavteino.ns: five plus blank control
_
Temperature: 19.1- 203C
Total suspended solids and pH on day of testing: 4.53 g/L (TSS in
final: 1.81 g/L), initial pH 8.0 and final pH 8.5
Element Basis: Respiration inhibition as determined by oxygen
consumption.
001348
Remarks: Values corrected to 20C for calculations.
R0 esw0 vs 00000000000 Nominal concentrations: Blank control, 10, 50, 100, 500, and 1000 mglL. The blank control and 1000 mg/L were duplicated.
Element values: 30-minute EC50 = >1000 mg/L. 3-hour EC50 = >1000 mg/L.
Element values based on nominal concentrations.
Remarks: Testing was conducted on the mixture as described in the Test
Substance Remarks field. The values reported apply to that mixture and not
the fluorochemical proportion alone.
-
Co0 nclu0 sions0000000 The FC-203FC 3-hour ECs for activated sludge respiration inhibition was determined to be >1000 mg/L. This concentration inhibited respiration by ~13%.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
pataQuauwY
0000
Reliability: Kiimisch ranking = 2. This study meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmationofthe amount of fluorochemical proportion in the solution.
REFERENCES
000000
`This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number R1335, 1997.
oTheR Last changed: 6/26/00
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001349
ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR
wr _R133S Date 3-24-97 Analyst TCS)
Page t_of
Type Test OECD ACTIVATED SLOOGE RESPIRATION Tl1i Tie on TEST Ha
ACTIVATEDSLUDGEMIXED LIQUOR
Date Obtained 3-34-97
Date Used 3-24-97
Mixed Liquor Suspended Solids (MLSS) 4.63 gL. Adjusted to __-- g/L.
MLSS in final test solution _1.{__ giL
i
Initial pH _T.D_ (1st test soln. prepared)
Final pH _$:5__ (last soln. prepared)':
Comments
--
TESTSUBSTANCE
Sample Description _FC-- 203FC AFFF
inital PH of highest conc. tested (tecomglL) = 1:5 Comments
Z Adjusted to pH _--_
'BEFERENCE SUBSTANCE Sample Description 3,5 Q1chloro Qheno
Initial pH of stock solution (SCD_ mgt) = 7.7
Comments
Adjusted to pH __--
EXPOSURECONDITIONS Test Duration Dhowes
Test Temperature Range: 1%} Comments
~~~
=0.3
0000000
_
SP
001350
ENVIRONMENTAL LABORATORY
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OECD ACTIVATED SLUDGE RESPIRATIONINHIBITIONTEST #209
TESTSOLUTIONS PREPARATION
Test Substance: FC-203 FC AFFF
Sample Description: Golden liquid
Anatyat: TS
~ owe: 3-24-97
|
TestConc. mgadded
--mol__ perS00ml
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001351
ENVIRONMENTAL LABOORRATORY
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OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
=
RAW DATA
Test Substance: [FC-208FC RAFF,
Source of Activated Sludge Mixed Liquor: Meteo Lsde (Ogfer Tread meet PlawdSt. fae
Date Received: _3 = 249-91
Date Used: 3-34-97 MISS
Analyst: Tus
,
30-MINUTE EXPOSURE
Test Subsiancs|
Concentration(rglc) 0.5
DISSOLVED OXYGEN CONCENTRATIONS, mglL
~
TIMEMINOTES
ol
Fomp
1.5
25 )30)35 [40 [45 [5.055]
BKCONTROL|
SO.
70 S70o0o0 BKCON/TR0O0LI0
wu [2Jason [cs[sas Tunfsa ing
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I[ese1 l ei4 [so4 ]sal |lu@s3]3 [uas4[[. v1ao]]g aaao[] e3a]a3 5=]a[2 4=20[19.09
3-HOUR EXPOSURE [-- TestSubstance Concentration (ogi)
DISSOLVED OXYGEN CONCENTRATIONS, mg/L. TE --
TIME - MINUTES
1.0 | 1520 )|25)3.0|35 4.0 45) 50(
:
TY sm--.
Temp
55
c
he[olales]eilealsaTuas ual sq [0a BKCoNTROLT
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bal 6al59 sss ys] H2]37(33029/26 [202
50 168]S[ea5g 54 sold. 42[38134[29(199
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#om Tau[vefra [raled calss [sulsol4e[ual rae
pH of activated sludge mixed liquor: Initial _%.
Final_&
PH of highest concentration tested: Initial13
Final_.5
A
Test temperature: (93 ~ 30.72
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001352
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ENVIRNOMENTAL LABORATORY re R13a 3
OECD ACTIVATTEEDSTSLSUODLGUTEIROENSSPIPRRAETPIAORNAITNIHOINBITION TE#S20T9
Tost substance: 3,5 Dichlore phenol sample Dascrpion: Aldrich (red label) lot * 09615 Ke DE Anas: {eS
3 Date: 3-34-97
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001354
: LR R325 ENVIRONMENTAL LABORATCRY 2a ATA PGory OECD ACTIVATED SLUDGE RESPIRATICN INHIBITICN "EST #209
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Test Substance: 3,5: Qichlone phenol
Source of Activated Siudgo Mixed Liquor:Metrolongte(aeterTreplment Plawck St{.
Date Received: _3 - 2427
Date Used: _3-2499 =
Analyst: __T&S
-
30-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mglL
lug[agTra ToyTegJos [eo [safs, [an]an [ioe BoNeLT Test Substance
Concanatien (yl)
TIME-MINUTES
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Temp
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DISSOLVED OXYGEN CONCENTRATIONS, mg.
3
Test Substance
Concentration (mpl)
1.5
TIME - MINUTES
2.53035 4.0 45| 5.0
Temp
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