Document emjkz9O2ykJkJQ2jvqXVZonEE

-4 5 R C V 12 79 < M I> ESTABLISHED IBOi E I. du P o n t de N e m o u r s & C o m p a n y IN C O RPO R A TED W il m in g t o n , De l a w a r e 19898 POLYM ER PRODUCTS DEPARTMENT EXPERIMENTAL STATION PERSONAL AND CONFIDENTIAL cc: A. J. Dahl B. W. Karrh L. J. Papa Pral File I.C. - 353 - NI 1400 - 269 A/ZZZB- IS T 1 May 20, 1981 TO: DR. J. M. HEDGES - PPD, Circleville, Ohio FROM: S . S . STAFFORD ANALYSIS OF BLOOD SAMPLES FOR PERFLU0R00CTAN0ATE (Job No. 810-633; PRAL Nos. 81-1936-1950; Notebook Nos. E22514.E26238) As requested in your letter of 5/1/81 to L. J. Papa, the 16 blood samples submitted then have been analyzed' for perfluorooctanoate (Cg). Results and sample identification are given in the attached table. As noted there, the analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 10% relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), -with a detection limit of 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment jah Key Words: P erflu orooctan oic Acid Perfluorooctanoate Blood Analysis GC Thi-rr-'s- a world o! Ilu'-os we rp domo somethina about 000083 EXP000022 EID713836 TABLE I CONCENTRATION OF PERFLllOROOCTANOATE IK BLOOD ( a ) Sample PRAL No. 81-1936 81-1937 81-1938 81-1939 81-1940 81-1941 81-1942 81-1943 81-1944 81-1945 81-1946 81-1947 81-1948 81-1949 81-1950 81-1951 Date Sampled 5/1/81 5/1/81 5/1/81 5/1/81 5/1/81 5/1/81 5/5/81 5/4/81 5/4/81 5/4/81 5/4/81 5/4/81 5/4/81 5/5/81 5/5/81 5/5/81 P.R.No. 1485 3518 2430 3032 5656 6828 3322 0275 2718 3766 4748 5646 7350 1505. 5645 7452 Name GC Analysis Date Analyzed 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/12/81 5/13/81 5/13/81 5/13/81 5/13/81 5/13/81 5/13/81 [Cr ], DR F/g bl 0.027 n.d. 0.017 0.015 n.d. n.d. 0.030 0.027 0.025 n.d. 0.017 0.026 0.D2B 0.008 0.053 0.017 (a) Analysis as described in Lab Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.688 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 pgF/g. The detection limit is A 0.004 (JgF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [Cg] < 0.004 ppm, which cannot be distinguished from reagent background. ^ REDACTED* EXP000023 EID713837 000084