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Industria1l81B 0 FI -RTOENO STTAGLEaboRrOatAoDries, Inc.
NORTHBROOK, ILLNGIS 40062
Tagen Cseeed 2080
REPORT TO MINNESOTA MINING AND MANUFACTURING COMPANY
ACUTE ORTAWLOTMOXAITCEIRTIYALSSTUDIES ON IBT NO. A4dl4
SMaudbejeAcvtatoiParboileebcyiv3eMOrodrerInIsnpPecatiiomneavrn.d3CM.opyNoi.ngC2a.s0C4o-n6f3id0e9ntial Information:
105533..000011
EExxhhiibbiitt
en | 1053
State of Minnesota v. 3M Co.,
[tieoi Court File No. 27-CV-10-28862
emnorresses
ndustial BVO TEST Labosatosics, Joc.
REPORT TO MINNESOTA MINING AND MANUFACTURING COMPANY
ACUTE ORAL TOXICITY STUDIES ON TWO MATERIALS IBT NO. Addl4
I Introduction Samples of two materials identified as L-1931 (20470-23F) and L-1932
(20430-39D) respectively, were received from Minnesota Mining and Manufacturing Company for the purpose of conducting acute oral toxicity studies employing albino rats as test animals. IL Procedure
The procedure followed in the evaluation of each test material was the same and is described below.
Healthy, young albino rats of the Sprague-Dawley strain with a body weight range from 150 to 200 grams were used as test animals. The rats were divided into groups of four animale each (two male and two female) for dosing purposes.
All animals used were kept under observation for five days prior to experimental use, during which period they were checked for gencral physical health and suitability as test animals. The animals were housed
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3MAO1789596
SdutBIrOi-TaESlTLaboratories, Jc
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in stock cages and permitted a standard laboratory rat diet# plus water
2d libitum until 16 hours immediately prior to oral intubation.
On the morning of the first test day, after a 16-hour fast (water
permitted), the selected dose groups of four rate each (two male and two female) were intubated with previously calculated doses of the test material in the form of an aqueous suspension, All doses were
administered directly into the stomachs of the rats using a hypodermic syringe equipped with a ball-pointed intubating needle.
Following oral administration of the test material, the rats were housed individually in observation cages ( 10" x 8 x 8%) and observed for the succeeding 30 days. All mortalities and/or reactions displayed were recorded.
Arrangements were made to autopsy any animal which might succumb during the study as well as all surviving animals at the end of the 30 days.
At the end of the observation period, all data were collected and arrangements were made to calculate the acute oral mean lethal dose (LD50) of the test material using the techniques of Weil#, Thompsont#, and Thompson and Weilesrs,
* #
Wayne Weil,
CLaarbroBlloS.x,
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CIalllicnuoliast.ion
of
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EBfifoemcettirviecsD,osSeep(tL.D51g95o2x. EDgq) and Instructions in Their Use,
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Thompson, to Estimate
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aNnodv.Int1e9r4p7.olation
Thompson, William R. and Weil, Carrol S. ; On the Construction
of Tables for Moving Average Interpolation. Biometrics, March 1952,
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MA1789507
Industrial BAO TEST LaboratoriIensc,.
3
II. Results A. Mortality The mortality data are presented in Tables I and IL
TABLE 1
TEST MATERIAL: L-1931
Acute Oral Toxicity - Albino Rats
Mortality Data
Doser Number
(g/kg)
Dead
Number Tested
14
1
4
2.0
1
4
3.0
2
4
4.6
4
4
EE
AStcauntdearOdraDlevLiDastgion= 2of.6LDg/gkgg= # 0. 6 g/kg
PerGent Dead 25 25 50 100
* Tsuhsepetnessitomna.terial was administered as a 25 per cent (w/v) aqueous
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IMA01T83598
Industial BAO ~TEST LaloraloicIsc,.
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TABLE Il
TEST MATERIAL: L-1932 Acute Oral Toxicity - Albino Rats
Mortality Data
B(oew/ge)r
NuDemabder| NuTemsbteedr
PerDeGaedmt
0.6
0
1
0.9
2
4
50
14
4
4
100
2.0
4
+
100
r-- er--e ------------------------------t
ASctuatnedaOrrdaDlevLiDagtgion= 0of.9LDg/skgg= +0. 1 g/kg + The test material was administered as a 10 per cent (w/v) aqueous
suspension.
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B. Reactions Summaries of the untoward behavioral reactions and time of death
following oral administration of the respective test materials are presented
in Tables 111 and IV. Necropsy of animals which died during the study as well as those
sacrificed at the end of the 30-day observation period revealed no significant gross pathologic alterations in the tissues and organs examined.
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Iv. Summary Acute oral toxicity studies employing albino rats as test animals
were conducted on two materials identified as L-1931 (20470-23P) and L-1932 (20430-39D) respectively. The test materials were administered in the form of aqueous suspensions. The acute oral mean lethal dose values (LDso), expressed in terms of undiluted test
material, were 2.6 0.6 g/kg for L-1931 and 0.9 0, 1 g/kg for
L-1932 respectively.
Respectfully submitted, INDUSTRIAL BIO-TEST LABORATORIES, INC.
Report prepared by: DepGaarraltdmSecnhtoaelniDgi,reBc.tSo.r Acute Toxicity Department
Report approved by: ODtiirsecEt.orFaacher, Ph.D.
0) 6.6 atau"
Pr. Ce.sidCeanltandra, Ph.D., M.D.
nSiecptember 21, 1966
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105533..0009
MA01789603