Document emZbDJG2dDmOy5VBOLdaRNorG

ACUTE TOXICITY TO AQUATIC PLANTS (ALGAE) TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be referred to as PFOSAA, FC-128 or as the major component of FC-127, FC-129, FC-109, FC-109-X or R1904. (Glycine, Nethyl-N-[(heptadecafluorooctyl)sulfonyl]-, potassium salt, CAS # 2991-51-7) Remarks: The test sample is FC-129 referred to by the test laboratory as R1904. Current information indicates it is a mixture of 42% test substance, 32% water, 14% 2-butoxyethanol, 4% ethanol, and 8% C4-C; fluoroalkyl carboxylate compounds. Test material was from 3M production lot 930014, mfg. date 3/97. The following summary is abbreviated because the test sample is a `mixture of the test substance Data may not accurately relat in a water/o e toxicity of rganic solvent s the test sample oluti with on. that of the test substance. METHOD: Methods: OECD 201 Test type: Static acute GLP: No Year Completed: 1997 Species: Selenastrum capricornutum Analytical monitoring: Temperature, pH, and conductivity RESULTS Nominal concentrations: Bk control, 129.6, 216, 360, 600, 1000 mg/L (4 replicates per concentrations, 6 per blank controls) Element values: 9r6%eCtonf(imdegn)ce [57r 3 h-- -- otmwe -- sC =o, --] [T[ ohoo wCh [ ou tesa | r|E8 aC ssisao ,| | 000400 - rent ren tng 55% Confidence Average Specific| [2&4 hour8 EC,| |ho585u 05 | | rEs7arC a7seose0y| | Growth Rate 72howrECs, | 610 |store| [[9--6S6hhoowurNEOCEC | 261064 | | NostsApopsleiscabl| e] Remarks: Testing was conducted on the mixture of the test substance as described in the test substance remarks field. The values reported apply to that mixture and not the test substance. No attempt was made to determine the impact of the presence of the organic solvents or what portion of the toxicity can be contributed to the Perfluorooctanesulfonylamido(ethylacetate. CONCLUSIONS The FC-129 96-hour ECs; for Selenastrum capricomutum was determined to be 298 mg/L (C.1.: 278-319 mg/L) by Cell Count and 604 mg/L (C.I.: 550-664 mg/L) by Average Specific Growth Rate. The 96-hour no observed effect concentration for both Cell Count and Average Specific Growth Rate was 216 mg/L. Effect determined to be algistatic based on recovery of cells previously exposed to the 1000 mg/L concentration. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmationoftest substance concentrations. There is a lack of characterization of the test sample. REFERENCES `This study was conducted at AScl Corporation of Duluth, Minnesota at the request of the 3M Company. OTHER Last changed: 5/17/00 000401 oT EAn5v6i1AoCSnonTpcoSarntiyTonoToinAgS0eD1Di0uv0ili6ao8sn STUDY TITLE GROWTH INHIBITION OF R1904 FOR `GREEN ALGA (Selenastrum capricornutum) DATA STANDARD `OECD GUIDELINE 201 STUDY COMPLETED June 18, 1997 TESTING FACILITY AScl Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDYIDENTIFICATIONNUMBERS AScl Study ID# 5010-068 3M Company Lab Request R1904 SShpoomner:Sl3yMY C1o8mpRa1n3y08 . 1 000402 EASmlCaoorpnoSryiToan1/nAgS3eD0D1i5uo.9sn8 1.0 GENERAL INFORMATION Study Title Sponsor `Sponsor's Representative EE `Testing Facility { principal Investigator [Poicctbieor Definitive Test Dates Data Validation and Report Review Retention of Raw Data and Final Report | Location of Raw Data and Final Report Growth Inhibition of R1904 for Green Alga | (Selenastrum capricornutum). OECD Guideline 201. 3M Environmental Technology and Safety | Services, Building 2-3E-09, 935 BushAvenue, St. Paul, MN 55133; Tel No. (612) 778-7452. aSBnuudssahSnaAfBveeetanycuhSe,e,r3vSitMc.esEP,anuvBliu,riolMndmiNenng$t25a-1l335T3-e;0c9h,no9l3o5gy Tel No. (612) 778-7452. AScl Corporation/AScI-Duluth Environmental "Testing Division, 4444 AirparkBoulevard, [Lm[ [ oecaT ssoi o sdo m ton g] u] e|| Duluth, MN 55811; Tel. No. (218) 722-4040. `Alan Mozol WX J Report A Signature; Two years from the date study is completed. Testing Division, 4444 Airpark Boulevard, | AScl Corporation/AScI-Duluth Environmental Duluth, MN 55811; Tel. No. (218) 722-4040. [ Shona So-- y IF 1304 . 2 000403 EAniSroCnonrcpoourlioTnu/AnSgelDiDvuilsuotnh "ASE Sudy IDF 5010.68 2.0 OBJECTIVE(S) To determine the 3-d and 4-d effect concentrations (ECy''s and ECsy's) and the 4-d no f| o(bSseelrevnaabslteruemffceacptrciocnocrenuttruamt)iounn(deNrOEstCa)tiotfestthecotnedsittsiuonbss.tance, R1904, for green alga This study was conducted according to OECD Guideline 201 (OECD 1993). I 3.0 TEST METHODS { Test Substance | [ Test Organisms ft Algal Medium Exposure Chambers | | Pstraobpleer,tiaensd: cRom1p9l0e4t,elaymbsoelrublliequiind,wastpeerc.ific gravity ca. 1.3 (water = 1), Test concentrations: 0 (algal medium control), 129.6, 216, 360, 600, and 1000 mg/L. A 2000 mg/L stock solution was prepared by mass addition to amlagdale mbeydvioulmumfeolaldodwietdiobnyomfetchheansitcoaclk tsotiarlrignagl. meEdaicuhmt.est concentration was. | Source: ATCC 22662 rIneoccoumlmuemnpdreedpamraetdiioun:m fAolrg4aldastyoscbkewfaorsestuebsctuilntiutriaetdioinn. OECD oUbsteaiinnatnesti:nitSiatloccekllcudletnusrietywaosf d1i.l0utXed1a0n*dceslpliskpeedrinmtlo (t1e.st0sXolu1t0i%onascttuoal concentration). | Prepararion: Deionized water was spiked with nutrient stocks (including Na,EDTA) to OECD recommended concentrations. The most recent chemical analysis for deionized water is in Raw DataPackage. 0C.ha2r2acytmerifsitlitcrsat:ionAtprtieosrt time, to use the medium in the test. had a pHof 7.86. Sterilized via Type: sealed Sterilized with foam via autoclave stoppers. 250-ml borosilicate-glass Erlenmeyer flasks Use in test: 100 ml of algal medium o test solution/chamber. Six control replicates and four employed. During replicates per the test, kept test substance concentration were. stoppered, except when experimental observations were made. SSppoonnssoorr.Su3dMy CIoDm#pRainSy04 - 3 000404 Observations Endpoint Calculations EAnvSioACnSomrTepnoSanltioyTni/DAn4Sge3tD0Di1vu0l0ti6ot8n Duration: 4 days. Daily photoperiod: Continuous at 740 ft-candles using cool-white fluorescent lamps. Test chambers held on shaker table set at 100 pm. Temperature: 25.0 + 2C. | Biological: Daily cell counts using direct microscopic enumeration via Improved Neubauer hemacytometer. aWnadtetremcpheermaitsutrrey,: ((21))dAaitlyt-estteimnpietriaattiuorne.andAltlerdmeitneartmiionna-tiPoHn,s cwoenrdeucmtiavdiety, | according to APHA methods (1995). I-, 2-, 3-, and 4-d ECsy's, 3- and 4-d EC,'s, and 4-day NOEC's based on both cell counts and average specific growth rates (x). Statisticalanalyses performed (NOECs) aunsidngEPTAriPmrmoebidt Sspoefatrwamraen-(KEaCrjob'esr).Method (ECsq's), Toxstat SShpootnS sor: So3tMr CN ompany . ` 000405 EAnSvcilroCnomrcpootrauiToens/tiAnSgekD-iDviusliaohn 'ASel Sudy IDI 5010068 4.0 RESULTS TinesTtabSluebsst1a,nc2e, Taonxdic3i)ty (Pertinent daaaxe | 1Cel1l-dCoEuCnst;1d58E6Camgg:/L43(1524m-g6/5L6,).(385-450, 1Ce2lldCoEuCnt;2-8d5E9Cmsg:/L39(5777m-g9/50L).(356-439). 1Ce3lldCoEuCnt:3-2d3E4Cymgg;/L1(11397m-g2/68L).(94-130). 1Ce3lldCoECuyngs;3-6d1E0Cmyg:/L24(5851m-g6/76L).(225-272). 1Cel4ldCEoCywg;4-248E7Cymgg:/L1(62348m-g3/2L2.).(54229. | I 1Ce4lldCoECuyn;t;4-6d0E4Cgmyg:/L29(855m0-g6/4L).(278-319). | 4Cel4ldCoNuOnEtC4:-d2N1O6EmCg:/L.216 mg/L. l Test Conditions| Effect was recoveryof dceetllesrmpirneevdiotuoslbye eaxlgpiossteatdictobatsheed on 1000 mg/L test substance concentration. Temperature (C): 24.826.0. Light intensityfi-cdls: 740. PH: 6789.46. { i} _| Specific conducrivi os/cm): 123-189. SSppoonnssoorr:Su3dMy CIoDmApRaInSyO4 : s 000406 EAavlir"CoAoumrSepnoSarulaidTosnIi/FnASge0D1D0iu0o6hn8 5.0 TEST QA/QC QAQC Cries EN Algal counts in control must increase by at | During the first 3d of the test control algal [ elexapsotsuarfea.ctor of 16 during the first 3 d of the counts increased by a factor of 348. Test duration must be 4 d. The test duration was 4 d. f 6.0 REFERENCES WAamteerricaanndPWuabsltiecwHaetaelrt.hAAPssHoAc,iatWiaosnhi(nAgPtHoAn),.D.1C99.5. Standard Methods for the Examination of Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for `Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Hamilton, M.A., R.C. Russo, and R.V. Thurston, 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in `Toxicity Bioassays. Environ. Sci. Technol. 11(7): 714-719; Correction 12 (4): 417 (1978). U.S. Environmental Protection Agency (EPA). 1988. Computer Program Probit. Biological Methods Branch, EMSL, U.S. EPA, Cincinnati, OH 45268. SSpponosonr:Sa3udMyICompRa1n5y04 .6 000407 TABLE 1. tcEoor rmsino IEnChi'bistiEoCnsTye'sSt, w9it5h%RC1o9n0f4idence Intervals, and 4-d NOEC''S from Algal Growth | mma es | cams [mwem e me T ] ew ooemm om w ea T ee m Tm] [m | 6 ooe man] me B erR RTL eR s] LRAERRY| [ pCooemmes- [o woe1e em4eEeCes,, T I|m T E w E --a r [ssw ] ] ee | 1 [ovo [36 iBa emtemit one] Fr----, , 000408 EAnSvCilroCnomernptoarluiToens/tiAnSgelD-iDvuilstohn `ASI Sudy TDF 010.068 TABLE 2. cCealplrsicpoerrnmutlumanEdxpPoesrceednttoagRe1D9i0f4ference from Mean Control Value for Selenastrum | 8 |e [RTS Tesh tCono o | pe AB mns cb 55 EF 107 Mean 850) AB 55 bc 77 i Mean 7.3 (17) xB 107 | Dc 65 I Mean. 7.8 (11) AB 53 bc s3 Mean 43651) f AB 33 Mebcan 35 43(60) { AB 21 { Dc 21 Mesa 15 (89) rr eT -- y a 5a st 614 $580) So 250 48.8 (-9) i3s7 " 41.3 (23) z2 2652(51) P2)% F1esd 20.8 (61) 30062 34386 336960 348.00) 2=64 303108 291.0 (-16) 22004 71948 219.0 (37) 77 98% 8.07) 7 320 885) +2 23 28.99) 700s4 sIz | 672276 { 110) E50T6 a71 583.0 (-9) ! sstm | E50d4 563.8 (-12) 115643 116655 156.8 (76) E2l 230 32.8 (95) 23 34 30100 | * Parenthtic Values = %Differencefrom Control Mean Value *0-dMeanAlgalCount = 1X.10 04 SSppoonnssoorr:Su3dyCIoDmFpRa1ny04 . 8 000494 TABLE 3. UC rmom w= EAmSierl"oCAooSmrCpeoSctrtuadiTyoen1/0nA2Sge5lD0-i1Dv0iu0sai6to8hn VAavleureagfeorSpSeecliefniacsGtrruomwtchapRraitceosrn(u4t)uamnEdxpPoesrceednttoagRe1D9i0f4ference from MeanControl A emon EE TTR memes] [= mo [oe[ee|oe | ENE f 1495 (3) I Les an [I =[me [ore L465 (29) i 1 [oe[|{ |[we [oe[we [oe | "k= CellsoeInrCmoiin, bt Parcathetic Values = % Difference from Control Mean Value #04 Mean Ala Count = 10 X 10 SSppoonnssoorr:SdIMy CDoFmpRa1n3y04 --- . 5 000414 Co