Document em46xy6Gp8qgkKjJaGjENEyN9

BACK TO MAIN ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE_________________________________________ Identity: Perfluorooctanoic acid, tetrabutylammonium salt; may also be referred to as PFOA tetrabutylammonium salt, tetrabutylammonium perfluorooctanoate, or as a major component of L-13492. (Octanoic acid, pentadecafluoro-, tetrabutylammonium salt, CAS # 95658-53-0) Remarks: The 3M production lot number was 2327 The test sample is referred to by the testing laboratory as L-13492. The T.R. Wilbury study number is 840-TH. The 3M Environmental Laboratory Request Number is N2332. The purity of the sample was not sufficiently characterized, although current information indicates it is a solution of 44.9% tetrabutylammonium perfluorooctanoate, 27.9% water, and 27.2% isopropanol The following summary applies to the test sample as a mixture of the test substance in water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD Method: U S. EPA-TSCA Guideline 797.1300 Test type: Acute static GLP: Yes Year Completed: 1995 Species: Daphnia magna Supplier: Obtained from cultures maintained by T.R. Wilbury Laboratories Inc, Marblehead, MA from an original culture from Aquatic Research Organisms, Hampton, NH. Analytical monitoring: DO, conductivity, pH, and temperature were monitored daily. Exposure period: 48-hours Test organism age: < 24-hours Statistical methods: LC50 and EC50 values calculated, when possible, by probit analysis, moving average method or binomial probability with non linear interpolation using the computer software of C.E. Stephan. Test conditions Dilution water: Deionized water adjusted to a hardness of 160-180 mg/L as CaCO3/L Dilution water chemistry: Not given. BACK TO MAIN Lighting: Cool-white fluorescent bulbs with an intensity of 20 ft-c. Photoperiod of 16-hours light, 8-hours dark with a 15 minute transition period. Stock and test solutions preparation: A 100 mg/L primary stock solution was prepared in dilution water. After mixing, the primary stock was proportionally diluted with dilution water to prepare the test concentrations. No insoluble material was noted during the test. Exposure vessels: 300 mL glass beakers containing 250 mL of test solution. The approximate depth of test solution was 9 cm. The vessels were loosely covered during the test. Number of replicates: 2 Number of daphnids per replicate: 10 Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 48 hours): 8.5 - 8.7 mg/L (control exposure) 8.5 - 8.7 mg/L (100 mg/L exposure conc.) Conductivity range (0 - 48 hours) 620 - 630 gmhos/cm (control exposure) 620 - 640 gmhos/cm (100 mg/L exposure conc.) pH range (0 - 48 hours) 8 .4 - 8.6 (control exposure) 8.5 - 8.6 (100 mg/L exposure conc.) Test temperature range (0 - 48 hours) 20.5 - 20.7C (control exposure) 20.5 - 20.6C (100 mg/L exposure conc.) Element basis: mortality and immobilization RESULTS Nominal concentrations: Blank control, 13, 22, 36, 60, 100 mg/L. Element value: 24-hour EC50 = 89 (60 - >100) mg/L 24-hour LC50 = >100 mg/L (CI not calculable) 48-hour EC50 = 34 (30 - 39) mg/L 48-hour LC50 = 77 (60 - 100) mg/L 48-hour NOEC = 13 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical component alone. BACK TO MAIN Cumulative percent immobilization (includes mortality): Nominal Test Conc., mg/L 24-hours 48-hours Control 13 22 36 60 100 0 0 5 0 0 70 0 0 5 55 100 100 Control response: satisfactory CONCLUSIONS The test substance 48-hour LC50 for Daphnia magna was determined to be 77 mg/L with a 95% confidence interval of 60 - 100 mg/L. The test substance 48-hour EC50 for Daphnia magna was determined to be 34 mg/L with a 95% confidence interval of 30 - 39 mg/L. The test substance 48-hour no observed effect concentration (NOEC) was 13 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. REFERENCES This study was conducted at T.R. Wilbury Laboratories, Inc., Marblehead, MA, at the request of the 3M Company, Lab Request number N2332, 1995. OTHER Last changed: 2/26/01