Document em0B2zyDa5XvmDGzMR6MrzzQe

AR 826 -- 0829 ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was not noted. The test sample is FC-600 (SCAS Treated Effluent*). Current information indicates FC-600 is a mixture of 1.0% PFOS, 81.20% water, 12.00% diethylene glycol butyl ether, 1.00% sodium octyl sulfate, 2.00% propane Sultone foamer, 1.00% sodium decyl sulfate, 0.85% xanthan gum, 0.1% N-(3chloroallyl) hexaminium chloride, 0.80% starch, and 0.05 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentration of impurities that was treated in a SCAS reactor. Data may not accurately reflect the toxicity of the fluorochemical componentofthe test sample. This study is the second of 3 daphnia studies conducted to determine the toxicity of FC-600 after being treated through a SCAS reactor. This study used the supernatant ofa mixed microbial culture that initially contained FC-600 at 667 mg/L*. The effluent was collectedafter 4 hours of treatment in a SCAS reactor. The inoculum used came from the Metro Wastewater Treatment Plant, St. Paul, MN. * The study references a "1,000 mg/L" concentration. This value is not accurate as it does not include dilution by the sewage mixed liquor. METHOD: Method: Not noted. Type: Static acute N GLP: No Year completed: 1977 Species: Daphnia magna Supplier: Not noted. Analytical monitoring: Dissolved oxygen Exposure period: 48-hours Test organism age: Not noted. `Statistical method: ECs calculated by graphing data and by probit - analysis. Test conditions: Dilution water: Carbon-filtered well water, aerated. Dilution water chemistry: Dissolved oxygen: 9.4 mg/L 002008 SLitgohctkinagn:dNotetstnostoedl.ution preparation: Volume/volume addition Exposure vessels: Not noted. Number of replicates: two NNuummbbeerr ooff dcoanpchennitdrsatpieornsr:epsliixca(tceo:ntr1o0l was written up as a separate sWtautdeyr) chemistry during the study: Dissolved oxygen (final): 5.6 mg/L (control) 0.6 mg/L (100% effluent exposure) RESULTS Nominal concentrations: 5.6%, 10%, 18%, 32%, 56%, and 100% Element values: 48-hour ECso = 12.6 (10-15) % Element values based on nominal concentrations dReesmcarrikbesd: iTnetshteinTgeswtasSucbosntdaunccteedReomnatrhkesSfCielAd.S tTrheeatveadlmuiextruerpeoratse.d applies to that mixture and not the fluorochemical proportion alone. CONCLUSIONS The SCAS treated FC-600 effluent 48-hour ECs, was determined to be 12.6% with a 95% confidence interval of 10 to 15%. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking = 3. This study lacks descriptionofthe . methodology used, reportingoftest conditions and information on the amount of test substance remaining after SCAS treatment. The sample purity was not propery characterized and the study lacks analytical confirmation of the amountoffluorochemical proportion in the solution. REFERENCES `This study was conducted by 3M Company, Environmental Laboratory, St. ~ * Paul, MN, 1977. OTHER Last changed: 6/16/00 002009 Ct, 740101 ENVIROANQUMAETNITCALTOENNTGCITNREYERIWoNnGk.LSArBaOReAtTORY ? sei, TypeTest 489 Hours --TOLERANCE STudy ? Test Organisa_rasmentumn Material Tested FC00Gitined) ~ p00 Avg. Weight sive Date Started 8/8/77 Tine Stared /5:30.m Plotted 165, 137 Date Completed. [2/22 Dilution Water_Carbon Filtered - ell Water (AERATED m 9.4 ms2) oe, Bo\nes no Survival pn pp 2 hes bo Survival ph ppm Geow s vo Survival pH ppm 96 hes v0 Survivel pi ppm wo Gb]wo[17WmJi] Joes] NS ow f; e Bono [so 1o T [Tes[Tlo au AJ] NVI] #os lo [TToo ThaT /IN EES SLo [1To[1 To TPA7TR '; Av`eroagme eTgeemn[pe0srDaturrre [GO0P|O74 F FEE9| 2 4 sl ose 0 whe D0. Omgcurcutstion Rt 100 EEE TT TI EE TT Tage > 2 . E S ER E E H TFTEH FH H HHH HL Hi I LE i FRseE--En RHrT Ee TEEETe AolaPEa TH g A l . E EE T EEEH Te i NET ETT = LES BE \ so T FaEieTihE BEST COPY AVAILABLE T6TwS eNS % Survi be AS Sine 0[o5:0 dio ais dee ey a, 002010 Se TaEA i. Fouar Be S SNEFRRCdRaE,EA iHSaERRIERRi EN,EhCFaePgRE AeC n e Na RGSORcBL EiAeoESneohaieSns ESUEER EET htvi Eele SO CONE SG BNLEeRmPE E EpRNaRRoNrG H oeTd TnLOa CEGn E GRR e T e EcTalER 05C 75 g EL r )T oo |EC OLY B MEEE o mee0a ea kTRhe n enT dl e Si aed oe \ Be TONEn R h TeTPn s (Ure Drone ne Bh aa Se Saba : 2 H: aBi sBaLit n ADSa I CoEe0a5cCeoonn clTOTRR ET R i DRIERN5A005S Sham hnksBANEFE NOI 0 DPRTECL on mE GSCHOREaE leC e le E 31 Eo Ne seasls NEE S Tieso sient, 0i.71a52n00 eHEe lee EAMoErEN are ae L e o e A i Te s EEl bO iao WEANRNEEL COE eR CRyen ve SBR Cam CS Sea SAWRREEG00 le ie nh LE e AREA 3REERe ReoAS REC Cat E a LE e nee ee aL BEST COPY AVAILABLE 002011